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4ce4

39S large subunit of the porcine mitochondrial ribosome

Method: ELECTRON MICROSCOPY
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1. Protein Identity and Related Structures Protein Identity & Related Structures

MRPL27

OrganismNot specified

UniProt F1RT79

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 36 RNA 2 PDB declaration: 38-meric（38） Consistent with all polymer counts Chain 0； UniProt 1–148 Not recorded MRPL28 × 1 （F1RGV8） MRPL47 × 1 MRPL30 × 1 （F1STE5） MRPL32 × 1 （I3LTC4） MRPL33 × 1 MRPL34 × 1 MRPL35 × 1 （F1SVC5） MRPL36 × 1 （I3LDE8） 16S Ribosomal RNA × 1 UNASSIGNED RNA × 1 MRPL2 × 1 （F1RRN2） MRPL3 × 1 （F1RX24） MRPL4 × 1 （F1S3J8） MRPL9 × 1 （I3LR45） MRPL13 × 1 （F1S286） MRPL14 × 1 （F1RQV7） MRPL15 × 1 （F1RSH0） MRPL16 × 1 （F1RI89） MRPL17 × 1 （F1RMQ4） MRPL18 × 1 （F1SB64） MRPL19 × 1 （I3LNJ0） MRPL20 × 1 （F1RJD0） MRPL21 × 1 （F1RY70） MRPL22 × 1 MRPL23 × 1 MRPL24 × 1 （F1RHJ1） MRPL38 × 1 （F1RW03） MRPL39 × 1 （F1SHP6） MRPL44 × 1 （F1SR64） MRPL45 × 1 MRPL49 × 1 MRPL52 × 1 ICT1 × 1 UNASSIGNED HELICES × 2 THIOREDOXIN FOLD × 1 UNASSIGNED HELICES × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY cryo-EM buffer：20 MM HEPES-KOH PH 7.9, 100 MM KCL, 1 MM DTT, 20 MM MGCL2；pH 7.9；20 MM HEPES-KOH PH 7.9, 100 MM KCL, 1 MM DTT, 20 MM MGCL2 cryo-EM vitrification conditions：Cryogen ETHANE；MANUAL BLOTTING FOLLOWED BY MANUAL PLUNGING INTO LIQUID ETHANE Resolution 4.90 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4CE4 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4V19 Structure of the large subunit of the mammalian mitoribosome, part 1 of 2 Deposited 2014-09-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 28 PDB declaration: 32-meric
Chain 0 1–148（148 aa）
Not recorded ZN ZINC ION × 2 MG MAGNESIUM ION × 169 ELECTRON MICROSCOPY
cryo-EM buffer 20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT；pH 7.4；20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；MIXTURE OF LIQUID ETHANE AND PROPANE
Resolution 3.40 Å
5AJ4 Structure of the 55S mammalian mitoribosome. Deposited 2015-02-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 83 PDB declaration: 89-meric
Chain B0 1–148（148 aa）
Not recorded MG MAGNESIUM ION × 351 ZN ZINC ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer 20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT；pH 7.4；20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；MIXTURE OF LIQUID ETHANE AND PROPANE
Resolution 3.80 Å
6GAW Unique features of mammalian mitochondrial translation initiation revealed by cryo-EM. This file contains the complete 55S ribosome. Deposited 2018-04-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 90 PDB declaration: 95-meric
Chain B0 1–148（148 aa）
Not recorded MG MAGNESIUM ION × 326 ZN ZINC ION × 6 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 3 GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 1 NA SODIUM ION × 1 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6GB2 Unique features of mammalian mitochondrial translation initiation revealed by cryo-EM. This file contains the 39S ribosomal subunit. Deposited 2018-04-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 59 PDB declaration: 62-meric
Chain B0 1–148（148 aa）
Not recorded MG MAGNESIUM ION × 216 ZN ZINC ION × 3 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 2 GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 1 NA SODIUM ION × 1 FME N-FORMYLMETHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6YDP 55S mammalian mitochondrial ribosome with mtEFG1 and P site fMet-tRNAMet (POST) Deposited 2020-03-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 89 PDB declaration: 94-meric
Chain B0 1–148（148 aa）
Not recorded MG MAGNESIUM ION × 326 ZN ZINC ION × 6 SPM SPERMINE × 3 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.00 Å
6YDW 55S mammalian mitochondrial ribosome with mtEFG1 and two tRNAMet (TI-POST) Deposited 2020-03-21 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 89 PDB declaration: 95-meric
Chain B0 1–148（148 aa）
Not recorded MG MAGNESIUM ION × 325 ZN ZINC ION × 6 SPM SPERMINE × 3 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.20 Å
7NQH 55S mammalian mitochondrial ribosome with mtRF1a and P-site tRNAMet Deposited 2021-03-01 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 93-meric
Chain B0 1–148（148 aa）
Not recorded MG MAGNESIUM ION × 323 ZN ZINC ION × 6 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.50 Å
7NQL 55S mammalian mitochondrial ribosome with ICT1 and P site tRNAMet Deposited 2021-03-01 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 93-meric
Chain B0 1–148（148 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 335 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.40 Å
7NSH 39S mammalian mitochondrial large ribosomal subunit with mtRRF (post) and mtEFG2 Deposited 2021-03-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 59 PDB declaration: 61-meric
Chain B0 1–148（148 aa）
Not recorded MG MAGNESIUM ION × 221 ZN ZINC ION × 3 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 2 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.20 Å
7NSI 55S mammalian mitochondrial ribosome with mtRRF (pre) and tRNA(P/E) Deposited 2021-03-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 93-meric
Chain B0 1–148（148 aa）
Not recorded MG MAGNESIUM ION × 327 ZN ZINC ION × 6 SPM SPERMINE × 3 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.60 Å
7NSJ 55S mammalian mitochondrial ribosome with tRNA(P/P) and tRNA(E*) Deposited 2021-03-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 87 PDB declaration: 93-meric
Chain B0 1–148（148 aa）
Not recorded MG MAGNESIUM ION × 324 ZN ZINC ION × 6 SPM SPERMINE × 3 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.90 Å

11 other PDB entries and 11 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name F1RT79_PIG
Isoform —
PDB entities 1
Chains and sequence ranges Author chain 0； PDBConstruct 1–148； UniProt 1–148

MRPL28

OrganismNot specified

UniProt F1RGV8

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 36 RNA 2 PDB declaration: 38-meric（38） Consistent with all polymer counts Chain 1； UniProt 1–256 Not recorded MRPL27 × 1 （F1RT79） MRPL47 × 1 MRPL30 × 1 （F1STE5） MRPL32 × 1 （I3LTC4） MRPL33 × 1 MRPL34 × 1 MRPL35 × 1 （F1SVC5） MRPL36 × 1 （I3LDE8） 16S Ribosomal RNA × 1 UNASSIGNED RNA × 1 MRPL2 × 1 （F1RRN2） MRPL3 × 1 （F1RX24） MRPL4 × 1 （F1S3J8） MRPL9 × 1 （I3LR45） MRPL13 × 1 （F1S286） MRPL14 × 1 （F1RQV7） MRPL15 × 1 （F1RSH0） MRPL16 × 1 （F1RI89） MRPL17 × 1 （F1RMQ4） MRPL18 × 1 （F1SB64） MRPL19 × 1 （I3LNJ0） MRPL20 × 1 （F1RJD0） MRPL21 × 1 （F1RY70） MRPL22 × 1 MRPL23 × 1 MRPL24 × 1 （F1RHJ1） MRPL38 × 1 （F1RW03） MRPL39 × 1 （F1SHP6） MRPL44 × 1 （F1SR64） MRPL45 × 1 MRPL49 × 1 MRPL52 × 1 ICT1 × 1 UNASSIGNED HELICES × 2 THIOREDOXIN FOLD × 1 UNASSIGNED HELICES × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY cryo-EM buffer：20 MM HEPES-KOH PH 7.9, 100 MM KCL, 1 MM DTT, 20 MM MGCL2；pH 7.9；20 MM HEPES-KOH PH 7.9, 100 MM KCL, 1 MM DTT, 20 MM MGCL2 cryo-EM vitrification conditions：Cryogen ETHANE；MANUAL BLOTTING FOLLOWED BY MANUAL PLUNGING INTO LIQUID ETHANE Resolution 4.90 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4CE4 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4V19 Structure of the large subunit of the mammalian mitoribosome, part 1 of 2 Deposited 2014-09-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 28 PDB declaration: 32-meric
Chain 1 1–256（256 aa）
Not recorded ZN ZINC ION × 2 MG MAGNESIUM ION × 169 ELECTRON MICROSCOPY
cryo-EM buffer 20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT；pH 7.4；20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；MIXTURE OF LIQUID ETHANE AND PROPANE
Resolution 3.40 Å
5AJ4 Structure of the 55S mammalian mitoribosome. Deposited 2015-02-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 83 PDB declaration: 89-meric
Chain B1 1–256（256 aa）
Not recorded MG MAGNESIUM ION × 351 ZN ZINC ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer 20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT；pH 7.4；20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；MIXTURE OF LIQUID ETHANE AND PROPANE
Resolution 3.80 Å

2 other PDB entries and 2 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name F1RGV8_PIG
Isoform —
PDB entities 2
Chains and sequence ranges Author chain 1； PDBConstruct 1–256； UniProt 1–256

MRPL30

OrganismNot specified

UniProt F1STE5

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 36 RNA 2 PDB declaration: 38-meric（38） Consistent with all polymer counts Chain 3； UniProt 1–161 Not recorded MRPL27 × 1 （F1RT79） MRPL28 × 1 （F1RGV8） MRPL47 × 1 MRPL32 × 1 （I3LTC4） MRPL33 × 1 MRPL34 × 1 MRPL35 × 1 （F1SVC5） MRPL36 × 1 （I3LDE8） 16S Ribosomal RNA × 1 UNASSIGNED RNA × 1 MRPL2 × 1 （F1RRN2） MRPL3 × 1 （F1RX24） MRPL4 × 1 （F1S3J8） MRPL9 × 1 （I3LR45） MRPL13 × 1 （F1S286） MRPL14 × 1 （F1RQV7） MRPL15 × 1 （F1RSH0） MRPL16 × 1 （F1RI89） MRPL17 × 1 （F1RMQ4） MRPL18 × 1 （F1SB64） MRPL19 × 1 （I3LNJ0） MRPL20 × 1 （F1RJD0） MRPL21 × 1 （F1RY70） MRPL22 × 1 MRPL23 × 1 MRPL24 × 1 （F1RHJ1） MRPL38 × 1 （F1RW03） MRPL39 × 1 （F1SHP6） MRPL44 × 1 （F1SR64） MRPL45 × 1 MRPL49 × 1 MRPL52 × 1 ICT1 × 1 UNASSIGNED HELICES × 2 THIOREDOXIN FOLD × 1 UNASSIGNED HELICES × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY cryo-EM buffer：20 MM HEPES-KOH PH 7.9, 100 MM KCL, 1 MM DTT, 20 MM MGCL2；pH 7.9；20 MM HEPES-KOH PH 7.9, 100 MM KCL, 1 MM DTT, 20 MM MGCL2 cryo-EM vitrification conditions：Cryogen ETHANE；MANUAL BLOTTING FOLLOWED BY MANUAL PLUNGING INTO LIQUID ETHANE Resolution 4.90 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4CE4 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4V19 Structure of the large subunit of the mammalian mitoribosome, part 1 of 2 Deposited 2014-09-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 28 PDB declaration: 32-meric
Chain 3 1–161（161 aa）
Not recorded ZN ZINC ION × 2 MG MAGNESIUM ION × 169 ELECTRON MICROSCOPY
cryo-EM buffer 20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT；pH 7.4；20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；MIXTURE OF LIQUID ETHANE AND PROPANE
Resolution 3.40 Å
5AJ4 Structure of the 55S mammalian mitoribosome. Deposited 2015-02-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 83 PDB declaration: 89-meric
Chain B3 1–161（161 aa）
Not recorded MG MAGNESIUM ION × 351 ZN ZINC ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer 20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT；pH 7.4；20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；MIXTURE OF LIQUID ETHANE AND PROPANE
Resolution 3.80 Å
6GAW Unique features of mammalian mitochondrial translation initiation revealed by cryo-EM. This file contains the complete 55S ribosome. Deposited 2018-04-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 90 PDB declaration: 95-meric
Chain B3 1–161（161 aa）
Not recorded MG MAGNESIUM ION × 326 ZN ZINC ION × 6 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 3 GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 1 NA SODIUM ION × 1 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6GB2 Unique features of mammalian mitochondrial translation initiation revealed by cryo-EM. This file contains the 39S ribosomal subunit. Deposited 2018-04-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 59 PDB declaration: 62-meric
Chain B3 1–161（161 aa）
Not recorded MG MAGNESIUM ION × 216 ZN ZINC ION × 3 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 2 GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 1 NA SODIUM ION × 1 FME N-FORMYLMETHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6YDP 55S mammalian mitochondrial ribosome with mtEFG1 and P site fMet-tRNAMet (POST) Deposited 2020-03-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 89 PDB declaration: 94-meric
Chain B3 1–161（161 aa）
Not recorded MG MAGNESIUM ION × 326 ZN ZINC ION × 6 SPM SPERMINE × 3 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.00 Å
6YDW 55S mammalian mitochondrial ribosome with mtEFG1 and two tRNAMet (TI-POST) Deposited 2020-03-21 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 89 PDB declaration: 95-meric
Chain B3 1–161（161 aa）
Not recorded MG MAGNESIUM ION × 325 ZN ZINC ION × 6 SPM SPERMINE × 3 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.20 Å
7NQH 55S mammalian mitochondrial ribosome with mtRF1a and P-site tRNAMet Deposited 2021-03-01 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 93-meric
Chain B3 1–161（161 aa）
Not recorded MG MAGNESIUM ION × 323 ZN ZINC ION × 6 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.50 Å
7NQL 55S mammalian mitochondrial ribosome with ICT1 and P site tRNAMet Deposited 2021-03-01 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 93-meric
Chain B3 1–161（161 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 335 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.40 Å
7NSH 39S mammalian mitochondrial large ribosomal subunit with mtRRF (post) and mtEFG2 Deposited 2021-03-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 59 PDB declaration: 61-meric
Chain B3 1–161（161 aa）
Not recorded MG MAGNESIUM ION × 221 ZN ZINC ION × 3 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 2 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.20 Å
7NSI 55S mammalian mitochondrial ribosome with mtRRF (pre) and tRNA(P/E) Deposited 2021-03-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 93-meric
Chain B3 1–161（161 aa）
Not recorded MG MAGNESIUM ION × 327 ZN ZINC ION × 6 SPM SPERMINE × 3 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.60 Å
7NSJ 55S mammalian mitochondrial ribosome with tRNA(P/P) and tRNA(E*) Deposited 2021-03-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 87 PDB declaration: 93-meric
Chain B3 1–161（161 aa）
Not recorded MG MAGNESIUM ION × 324 ZN ZINC ION × 6 SPM SPERMINE × 3 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.90 Å

11 other PDB entries and 11 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name F1STE5_PIG
Isoform —
PDB entities 4
Chains and sequence ranges Author chain 3； PDBConstruct 1–161； UniProt 1–161

MRPL32

OrganismNot specified

UniProt I3LTC4

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 36 RNA 2 PDB declaration: 38-meric（38） Consistent with all polymer counts Chain 5； UniProt 1–126 Not recorded MRPL27 × 1 （F1RT79） MRPL28 × 1 （F1RGV8） MRPL47 × 1 MRPL30 × 1 （F1STE5） MRPL33 × 1 MRPL34 × 1 MRPL35 × 1 （F1SVC5） MRPL36 × 1 （I3LDE8） 16S Ribosomal RNA × 1 UNASSIGNED RNA × 1 MRPL2 × 1 （F1RRN2） MRPL3 × 1 （F1RX24） MRPL4 × 1 （F1S3J8） MRPL9 × 1 （I3LR45） MRPL13 × 1 （F1S286） MRPL14 × 1 （F1RQV7） MRPL15 × 1 （F1RSH0） MRPL16 × 1 （F1RI89） MRPL17 × 1 （F1RMQ4） MRPL18 × 1 （F1SB64） MRPL19 × 1 （I3LNJ0） MRPL20 × 1 （F1RJD0） MRPL21 × 1 （F1RY70） MRPL22 × 1 MRPL23 × 1 MRPL24 × 1 （F1RHJ1） MRPL38 × 1 （F1RW03） MRPL39 × 1 （F1SHP6） MRPL44 × 1 （F1SR64） MRPL45 × 1 MRPL49 × 1 MRPL52 × 1 ICT1 × 1 UNASSIGNED HELICES × 2 THIOREDOXIN FOLD × 1 UNASSIGNED HELICES × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY cryo-EM buffer：20 MM HEPES-KOH PH 7.9, 100 MM KCL, 1 MM DTT, 20 MM MGCL2；pH 7.9；20 MM HEPES-KOH PH 7.9, 100 MM KCL, 1 MM DTT, 20 MM MGCL2 cryo-EM vitrification conditions：Cryogen ETHANE；MANUAL BLOTTING FOLLOWED BY MANUAL PLUNGING INTO LIQUID ETHANE Resolution 4.90 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4CE4 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4V19 Structure of the large subunit of the mammalian mitoribosome, part 1 of 2 Deposited 2014-09-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 28 PDB declaration: 32-meric
Chain 5 1–188（188 aa）
Not recorded ZN ZINC ION × 2 MG MAGNESIUM ION × 169 ELECTRON MICROSCOPY
cryo-EM buffer 20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT；pH 7.4；20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；MIXTURE OF LIQUID ETHANE AND PROPANE
Resolution 3.40 Å
5AJ4 Structure of the 55S mammalian mitoribosome. Deposited 2015-02-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 83 PDB declaration: 89-meric
Chain B5 1–188（188 aa）
Not recorded MG MAGNESIUM ION × 351 ZN ZINC ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer 20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT；pH 7.4；20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；MIXTURE OF LIQUID ETHANE AND PROPANE
Resolution 3.80 Å
6GAW Unique features of mammalian mitochondrial translation initiation revealed by cryo-EM. This file contains the complete 55S ribosome. Deposited 2018-04-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 90 PDB declaration: 95-meric
Chain B5 20–207（188 aa）
Not recorded MG MAGNESIUM ION × 326 ZN ZINC ION × 6 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 3 GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 1 NA SODIUM ION × 1 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6YDP 55S mammalian mitochondrial ribosome with mtEFG1 and P site fMet-tRNAMet (POST) Deposited 2020-03-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 89 PDB declaration: 94-meric
Chain B5 20–207（188 aa）
Not recorded MG MAGNESIUM ION × 326 ZN ZINC ION × 6 SPM SPERMINE × 3 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.00 Å
6YDW 55S mammalian mitochondrial ribosome with mtEFG1 and two tRNAMet (TI-POST) Deposited 2020-03-21 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 89 PDB declaration: 95-meric
Chain B5 20–207（188 aa）
Not recorded MG MAGNESIUM ION × 325 ZN ZINC ION × 6 SPM SPERMINE × 3 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.20 Å
7NQH 55S mammalian mitochondrial ribosome with mtRF1a and P-site tRNAMet Deposited 2021-03-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 93-meric
Chain B5 20–207（188 aa）
Not recorded MG MAGNESIUM ION × 323 ZN ZINC ION × 6 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.50 Å
7NQL 55S mammalian mitochondrial ribosome with ICT1 and P site tRNAMet Deposited 2021-03-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 93-meric
Chain B5 20–207（188 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 335 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.40 Å
7NSH 39S mammalian mitochondrial large ribosomal subunit with mtRRF (post) and mtEFG2 Deposited 2021-03-07 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 59 PDB declaration: 61-meric
Chain B5 20–207（188 aa）
Not recorded MG MAGNESIUM ION × 221 ZN ZINC ION × 3 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 2 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.20 Å
7NSI 55S mammalian mitochondrial ribosome with mtRRF (pre) and tRNA(P/E) Deposited 2021-03-07 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 93-meric
Chain B5 20–207（188 aa）
Not recorded MG MAGNESIUM ION × 327 ZN ZINC ION × 6 SPM SPERMINE × 3 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.60 Å
7NSJ 55S mammalian mitochondrial ribosome with tRNA(P/P) and tRNA(E*) Deposited 2021-03-07 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 87 PDB declaration: 93-meric
Chain B5 20–207（188 aa）
Not recorded MG MAGNESIUM ION × 324 ZN ZINC ION × 6 SPM SPERMINE × 3 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.90 Å
8OIN 55S mammalian mitochondrial ribosome with mtRF1 and P-site tRNA Deposited 2023-03-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 94-meric
Chain BH 1–207（207 aa）
Not recorded MG MAGNESIUM ION × 332 K POTASSIUM ION × 41 PHE PHENYLALANINE × 1 ZN ZINC ION × 3 FES FE2/S2 (INORGANIC) CLUSTER × 3 SPM SPERMINE × 1 SPD SPERMIDINE × 1 FME N-FORMYLMETHIONINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20mM HEPES-KOH pH=7.5, 100mM KCl, 20mM MgCl2, ~1% sucrose (from mtRF1 storage buffer)
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.60 Å
8OIQ 39S mammalian mitochondrial large ribosomal subunit with mtRF1 and P-site tRNA Deposited 2023-03-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 58 PDB declaration: 62-meric
Chain BH 1–207（207 aa）
Not recorded MG MAGNESIUM ION × 205 K POTASSIUM ION × 30 PHE PHENYLALANINE × 1 ZN ZINC ION × 2 FES FE2/S2 (INORGANIC) CLUSTER × 1 FME N-FORMYLMETHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20mM HEPES-KOH pH=7.5, 100mM KCl, 20mM MgCl2, ~1% sucrose (from mtRF1 storage buffer)
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.50 Å

12 other PDB entries and 12 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name I3LTC4_PIG
Isoform —
PDB entities 5
Chains and sequence ranges Author chain 5； PDBConstruct 1–126； UniProt 1–126

MRPL35

OrganismNot specified

UniProt F1SVC5

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 36 RNA 2 PDB declaration: 38-meric（38） Consistent with all polymer counts Chain 8； UniProt 1–188 Not recorded MRPL27 × 1 （F1RT79） MRPL28 × 1 （F1RGV8） MRPL47 × 1 MRPL30 × 1 （F1STE5） MRPL32 × 1 （I3LTC4） MRPL33 × 1 MRPL34 × 1 MRPL36 × 1 （I3LDE8） 16S Ribosomal RNA × 1 UNASSIGNED RNA × 1 MRPL2 × 1 （F1RRN2） MRPL3 × 1 （F1RX24） MRPL4 × 1 （F1S3J8） MRPL9 × 1 （I3LR45） MRPL13 × 1 （F1S286） MRPL14 × 1 （F1RQV7） MRPL15 × 1 （F1RSH0） MRPL16 × 1 （F1RI89） MRPL17 × 1 （F1RMQ4） MRPL18 × 1 （F1SB64） MRPL19 × 1 （I3LNJ0） MRPL20 × 1 （F1RJD0） MRPL21 × 1 （F1RY70） MRPL22 × 1 MRPL23 × 1 MRPL24 × 1 （F1RHJ1） MRPL38 × 1 （F1RW03） MRPL39 × 1 （F1SHP6） MRPL44 × 1 （F1SR64） MRPL45 × 1 MRPL49 × 1 MRPL52 × 1 ICT1 × 1 UNASSIGNED HELICES × 2 THIOREDOXIN FOLD × 1 UNASSIGNED HELICES × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY cryo-EM buffer：20 MM HEPES-KOH PH 7.9, 100 MM KCL, 1 MM DTT, 20 MM MGCL2；pH 7.9；20 MM HEPES-KOH PH 7.9, 100 MM KCL, 1 MM DTT, 20 MM MGCL2 cryo-EM vitrification conditions：Cryogen ETHANE；MANUAL BLOTTING FOLLOWED BY MANUAL PLUNGING INTO LIQUID ETHANE Resolution 4.90 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4CE4 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4V19 Structure of the large subunit of the mammalian mitoribosome, part 1 of 2 Deposited 2014-09-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 28 PDB declaration: 32-meric
Chain 8 1–188（188 aa）
Not recorded ZN ZINC ION × 2 MG MAGNESIUM ION × 169 ELECTRON MICROSCOPY
cryo-EM buffer 20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT；pH 7.4；20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；MIXTURE OF LIQUID ETHANE AND PROPANE
Resolution 3.40 Å
5AJ4 Structure of the 55S mammalian mitoribosome. Deposited 2015-02-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 83 PDB declaration: 89-meric
Chain B8 1–188（188 aa）
Not recorded MG MAGNESIUM ION × 351 ZN ZINC ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer 20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT；pH 7.4；20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；MIXTURE OF LIQUID ETHANE AND PROPANE
Resolution 3.80 Å
6GAW Unique features of mammalian mitochondrial translation initiation revealed by cryo-EM. This file contains the complete 55S ribosome. Deposited 2018-04-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 90 PDB declaration: 95-meric
Chain B8 1–188（188 aa）
Not recorded MG MAGNESIUM ION × 326 ZN ZINC ION × 6 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 3 GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 1 NA SODIUM ION × 1 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6GB2 Unique features of mammalian mitochondrial translation initiation revealed by cryo-EM. This file contains the 39S ribosomal subunit. Deposited 2018-04-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 59 PDB declaration: 62-meric
Chain B8 1–188（188 aa）
Not recorded MG MAGNESIUM ION × 216 ZN ZINC ION × 3 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 2 GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 1 NA SODIUM ION × 1 FME N-FORMYLMETHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6YDP 55S mammalian mitochondrial ribosome with mtEFG1 and P site fMet-tRNAMet (POST) Deposited 2020-03-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 89 PDB declaration: 94-meric
Chain B8 1–188（188 aa）
Not recorded MG MAGNESIUM ION × 326 ZN ZINC ION × 6 SPM SPERMINE × 3 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.00 Å
6YDW 55S mammalian mitochondrial ribosome with mtEFG1 and two tRNAMet (TI-POST) Deposited 2020-03-21 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 89 PDB declaration: 95-meric
Chain B8 1–188（188 aa）
Not recorded MG MAGNESIUM ION × 325 ZN ZINC ION × 6 SPM SPERMINE × 3 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.20 Å
7NQH 55S mammalian mitochondrial ribosome with mtRF1a and P-site tRNAMet Deposited 2021-03-01 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 93-meric
Chain B8 1–188（188 aa）
Not recorded MG MAGNESIUM ION × 323 ZN ZINC ION × 6 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.50 Å
7NQL 55S mammalian mitochondrial ribosome with ICT1 and P site tRNAMet Deposited 2021-03-01 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 93-meric
Chain B8 1–188（188 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 335 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.40 Å
7NSH 39S mammalian mitochondrial large ribosomal subunit with mtRRF (post) and mtEFG2 Deposited 2021-03-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 59 PDB declaration: 61-meric
Chain B8 1–188（188 aa）
Not recorded MG MAGNESIUM ION × 221 ZN ZINC ION × 3 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 2 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.20 Å
7NSI 55S mammalian mitochondrial ribosome with mtRRF (pre) and tRNA(P/E) Deposited 2021-03-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 93-meric
Chain B8 1–188（188 aa）
Not recorded MG MAGNESIUM ION × 327 ZN ZINC ION × 6 SPM SPERMINE × 3 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.60 Å
7NSJ 55S mammalian mitochondrial ribosome with tRNA(P/P) and tRNA(E*) Deposited 2021-03-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 87 PDB declaration: 93-meric
Chain B8 1–188（188 aa）
Not recorded MG MAGNESIUM ION × 324 ZN ZINC ION × 6 SPM SPERMINE × 3 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.90 Å

11 other PDB entries and 11 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name F1SVC5_PIG
Isoform —
PDB entities 8
Chains and sequence ranges Author chain 8； PDBConstruct 1–188； UniProt 1–188

MRPL36

OrganismNot specified

UniProt I3LDE8

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 36 RNA 2 PDB declaration: 38-meric（38） Consistent with all polymer counts Chain 9； UniProt 1–100 Not recorded MRPL27 × 1 （F1RT79） MRPL28 × 1 （F1RGV8） MRPL47 × 1 MRPL30 × 1 （F1STE5） MRPL32 × 1 （I3LTC4） MRPL33 × 1 MRPL34 × 1 MRPL35 × 1 （F1SVC5） 16S Ribosomal RNA × 1 UNASSIGNED RNA × 1 MRPL2 × 1 （F1RRN2） MRPL3 × 1 （F1RX24） MRPL4 × 1 （F1S3J8） MRPL9 × 1 （I3LR45） MRPL13 × 1 （F1S286） MRPL14 × 1 （F1RQV7） MRPL15 × 1 （F1RSH0） MRPL16 × 1 （F1RI89） MRPL17 × 1 （F1RMQ4） MRPL18 × 1 （F1SB64） MRPL19 × 1 （I3LNJ0） MRPL20 × 1 （F1RJD0） MRPL21 × 1 （F1RY70） MRPL22 × 1 MRPL23 × 1 MRPL24 × 1 （F1RHJ1） MRPL38 × 1 （F1RW03） MRPL39 × 1 （F1SHP6） MRPL44 × 1 （F1SR64） MRPL45 × 1 MRPL49 × 1 MRPL52 × 1 ICT1 × 1 UNASSIGNED HELICES × 2 THIOREDOXIN FOLD × 1 UNASSIGNED HELICES × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY cryo-EM buffer：20 MM HEPES-KOH PH 7.9, 100 MM KCL, 1 MM DTT, 20 MM MGCL2；pH 7.9；20 MM HEPES-KOH PH 7.9, 100 MM KCL, 1 MM DTT, 20 MM MGCL2 cryo-EM vitrification conditions：Cryogen ETHANE；MANUAL BLOTTING FOLLOWED BY MANUAL PLUNGING INTO LIQUID ETHANE Resolution 4.90 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4CE4 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4V19 Structure of the large subunit of the mammalian mitoribosome, part 1 of 2 Deposited 2014-09-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 28 PDB declaration: 32-meric
Chain 9 1–100（100 aa）
Not recorded ZN ZINC ION × 2 MG MAGNESIUM ION × 169 ELECTRON MICROSCOPY
cryo-EM buffer 20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT；pH 7.4；20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；MIXTURE OF LIQUID ETHANE AND PROPANE
Resolution 3.40 Å
5AJ4 Structure of the 55S mammalian mitoribosome. Deposited 2015-02-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 83 PDB declaration: 89-meric
Chain B9 1–100（100 aa）
Not recorded MG MAGNESIUM ION × 351 ZN ZINC ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer 20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT；pH 7.4；20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；MIXTURE OF LIQUID ETHANE AND PROPANE
Resolution 3.80 Å

2 other PDB entries and 2 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name I3LDE8_PIG
Isoform —
PDB entities 9
Chains and sequence ranges Author chain 9； PDBConstruct 1–100； UniProt 1–100

MRPL2

OrganismNot specified

UniProt F1RRN2

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 36 RNA 2 PDB declaration: 38-meric（38） Consistent with all polymer counts Chain D； UniProt 1–306 Not recorded MRPL27 × 1 （F1RT79） MRPL28 × 1 （F1RGV8） MRPL47 × 1 MRPL30 × 1 （F1STE5） MRPL32 × 1 （I3LTC4） MRPL33 × 1 MRPL34 × 1 MRPL35 × 1 （F1SVC5） MRPL36 × 1 （I3LDE8） 16S Ribosomal RNA × 1 UNASSIGNED RNA × 1 MRPL3 × 1 （F1RX24） MRPL4 × 1 （F1S3J8） MRPL9 × 1 （I3LR45） MRPL13 × 1 （F1S286） MRPL14 × 1 （F1RQV7） MRPL15 × 1 （F1RSH0） MRPL16 × 1 （F1RI89） MRPL17 × 1 （F1RMQ4） MRPL18 × 1 （F1SB64） MRPL19 × 1 （I3LNJ0） MRPL20 × 1 （F1RJD0） MRPL21 × 1 （F1RY70） MRPL22 × 1 MRPL23 × 1 MRPL24 × 1 （F1RHJ1） MRPL38 × 1 （F1RW03） MRPL39 × 1 （F1SHP6） MRPL44 × 1 （F1SR64） MRPL45 × 1 MRPL49 × 1 MRPL52 × 1 ICT1 × 1 UNASSIGNED HELICES × 2 THIOREDOXIN FOLD × 1 UNASSIGNED HELICES × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY cryo-EM buffer：20 MM HEPES-KOH PH 7.9, 100 MM KCL, 1 MM DTT, 20 MM MGCL2；pH 7.9；20 MM HEPES-KOH PH 7.9, 100 MM KCL, 1 MM DTT, 20 MM MGCL2 cryo-EM vitrification conditions：Cryogen ETHANE；MANUAL BLOTTING FOLLOWED BY MANUAL PLUNGING INTO LIQUID ETHANE Resolution 4.90 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4CE4 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4V19 Structure of the large subunit of the mammalian mitoribosome, part 1 of 2 Deposited 2014-09-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 28 PDB declaration: 32-meric
Chain D 1–306（306 aa）
Not recorded ZN ZINC ION × 2 MG MAGNESIUM ION × 169 ELECTRON MICROSCOPY
cryo-EM buffer 20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT；pH 7.4；20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；MIXTURE OF LIQUID ETHANE AND PROPANE
Resolution 3.40 Å
5AJ4 Structure of the 55S mammalian mitoribosome. Deposited 2015-02-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 83 PDB declaration: 89-meric
Chain BD 1–306（306 aa）
Not recorded MG MAGNESIUM ION × 351 ZN ZINC ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer 20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT；pH 7.4；20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；MIXTURE OF LIQUID ETHANE AND PROPANE
Resolution 3.80 Å
6GAW Unique features of mammalian mitochondrial translation initiation revealed by cryo-EM. This file contains the complete 55S ribosome. Deposited 2018-04-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 90 PDB declaration: 95-meric
Chain BD 1–306（306 aa）
Not recorded MG MAGNESIUM ION × 326 ZN ZINC ION × 6 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 3 GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 1 NA SODIUM ION × 1 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6GB2 Unique features of mammalian mitochondrial translation initiation revealed by cryo-EM. This file contains the 39S ribosomal subunit. Deposited 2018-04-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 59 PDB declaration: 62-meric
Chain BD 1–306（306 aa）
Not recorded MG MAGNESIUM ION × 216 ZN ZINC ION × 3 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 2 GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 1 NA SODIUM ION × 1 FME N-FORMYLMETHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6YDP 55S mammalian mitochondrial ribosome with mtEFG1 and P site fMet-tRNAMet (POST) Deposited 2020-03-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 89 PDB declaration: 94-meric
Chain BD 1–306（306 aa）
Not recorded MG MAGNESIUM ION × 326 ZN ZINC ION × 6 SPM SPERMINE × 3 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.00 Å
6YDW 55S mammalian mitochondrial ribosome with mtEFG1 and two tRNAMet (TI-POST) Deposited 2020-03-21 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 89 PDB declaration: 95-meric
Chain BD 1–306（306 aa）
Not recorded MG MAGNESIUM ION × 325 ZN ZINC ION × 6 SPM SPERMINE × 3 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.20 Å
7NQH 55S mammalian mitochondrial ribosome with mtRF1a and P-site tRNAMet Deposited 2021-03-01 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 93-meric
Chain BD 1–306（306 aa）
Not recorded MG MAGNESIUM ION × 323 ZN ZINC ION × 6 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.50 Å
7NQL 55S mammalian mitochondrial ribosome with ICT1 and P site tRNAMet Deposited 2021-03-01 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 93-meric
Chain BD 1–306（306 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 335 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.40 Å
7NSH 39S mammalian mitochondrial large ribosomal subunit with mtRRF (post) and mtEFG2 Deposited 2021-03-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 59 PDB declaration: 61-meric
Chain BD 1–306（306 aa）
Not recorded MG MAGNESIUM ION × 221 ZN ZINC ION × 3 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 2 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.20 Å
7NSJ 55S mammalian mitochondrial ribosome with tRNA(P/P) and tRNA(E*) Deposited 2021-03-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 87 PDB declaration: 93-meric
Chain BD 1–306（306 aa）
Not recorded MG MAGNESIUM ION × 324 ZN ZINC ION × 6 SPM SPERMINE × 3 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.90 Å

10 other PDB entries and 10 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name F1RRN2_PIG
Isoform —
PDB entities 12
Chains and sequence ranges Author chain D； PDBConstruct 1–306； UniProt 1–306

MRPL3

OrganismNot specified

UniProt F1RX24

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 36 RNA 2 PDB declaration: 38-meric（38） Consistent with all polymer counts Chain E； UniProt 1–399 Not recorded MRPL27 × 1 （F1RT79） MRPL28 × 1 （F1RGV8） MRPL47 × 1 MRPL30 × 1 （F1STE5） MRPL32 × 1 （I3LTC4） MRPL33 × 1 MRPL34 × 1 MRPL35 × 1 （F1SVC5） MRPL36 × 1 （I3LDE8） 16S Ribosomal RNA × 1 UNASSIGNED RNA × 1 MRPL2 × 1 （F1RRN2） MRPL4 × 1 （F1S3J8） MRPL9 × 1 （I3LR45） MRPL13 × 1 （F1S286） MRPL14 × 1 （F1RQV7） MRPL15 × 1 （F1RSH0） MRPL16 × 1 （F1RI89） MRPL17 × 1 （F1RMQ4） MRPL18 × 1 （F1SB64） MRPL19 × 1 （I3LNJ0） MRPL20 × 1 （F1RJD0） MRPL21 × 1 （F1RY70） MRPL22 × 1 MRPL23 × 1 MRPL24 × 1 （F1RHJ1） MRPL38 × 1 （F1RW03） MRPL39 × 1 （F1SHP6） MRPL44 × 1 （F1SR64） MRPL45 × 1 MRPL49 × 1 MRPL52 × 1 ICT1 × 1 UNASSIGNED HELICES × 2 THIOREDOXIN FOLD × 1 UNASSIGNED HELICES × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY cryo-EM buffer：20 MM HEPES-KOH PH 7.9, 100 MM KCL, 1 MM DTT, 20 MM MGCL2；pH 7.9；20 MM HEPES-KOH PH 7.9, 100 MM KCL, 1 MM DTT, 20 MM MGCL2 cryo-EM vitrification conditions：Cryogen ETHANE；MANUAL BLOTTING FOLLOWED BY MANUAL PLUNGING INTO LIQUID ETHANE Resolution 4.90 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

No other PDB entry for the same UniProt protein was found.

View Construct and Data Evidence
UniProt name F1RX24_PIG
Isoform —
PDB entities 13
Chains and sequence ranges Author chain E； PDBConstruct 1–399； UniProt 1–399

MRPL4

OrganismNot specified

UniProt F1S3J8

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 36 RNA 2 PDB declaration: 38-meric（38） Consistent with all polymer counts Chain F； UniProt 78–273 Not recorded MRPL27 × 1 （F1RT79） MRPL28 × 1 （F1RGV8） MRPL47 × 1 MRPL30 × 1 （F1STE5） MRPL32 × 1 （I3LTC4） MRPL33 × 1 MRPL34 × 1 MRPL35 × 1 （F1SVC5） MRPL36 × 1 （I3LDE8） 16S Ribosomal RNA × 1 UNASSIGNED RNA × 1 MRPL2 × 1 （F1RRN2） MRPL3 × 1 （F1RX24） MRPL9 × 1 （I3LR45） MRPL13 × 1 （F1S286） MRPL14 × 1 （F1RQV7） MRPL15 × 1 （F1RSH0） MRPL16 × 1 （F1RI89） MRPL17 × 1 （F1RMQ4） MRPL18 × 1 （F1SB64） MRPL19 × 1 （I3LNJ0） MRPL20 × 1 （F1RJD0） MRPL21 × 1 （F1RY70） MRPL22 × 1 MRPL23 × 1 MRPL24 × 1 （F1RHJ1） MRPL38 × 1 （F1RW03） MRPL39 × 1 （F1SHP6） MRPL44 × 1 （F1SR64） MRPL45 × 1 MRPL49 × 1 MRPL52 × 1 ICT1 × 1 UNASSIGNED HELICES × 2 THIOREDOXIN FOLD × 1 UNASSIGNED HELICES × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY cryo-EM buffer：20 MM HEPES-KOH PH 7.9, 100 MM KCL, 1 MM DTT, 20 MM MGCL2；pH 7.9；20 MM HEPES-KOH PH 7.9, 100 MM KCL, 1 MM DTT, 20 MM MGCL2 cryo-EM vitrification conditions：Cryogen ETHANE；MANUAL BLOTTING FOLLOWED BY MANUAL PLUNGING INTO LIQUID ETHANE Resolution 4.90 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4CE4 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4V19 Structure of the large subunit of the mammalian mitoribosome, part 1 of 2 Deposited 2014-09-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 28 PDB declaration: 32-meric
Chain F 1–294（294 aa）
Not recorded ZN ZINC ION × 2 MG MAGNESIUM ION × 169 ELECTRON MICROSCOPY
cryo-EM buffer 20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT；pH 7.4；20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；MIXTURE OF LIQUID ETHANE AND PROPANE
Resolution 3.40 Å
5AJ4 Structure of the 55S mammalian mitoribosome. Deposited 2015-02-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 83 PDB declaration: 89-meric
Chain BF 1–294（294 aa）
Not recorded MG MAGNESIUM ION × 351 ZN ZINC ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer 20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT；pH 7.4；20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；MIXTURE OF LIQUID ETHANE AND PROPANE
Resolution 3.80 Å

2 other PDB entries and 2 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name F1S3J8_PIG
Isoform —
PDB entities 14
Chains and sequence ranges Author chain F； PDBConstruct 20–215； UniProt 78–273

MRPL9

OrganismNot specified

UniProt I3LR45

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 36 RNA 2 PDB declaration: 38-meric（38） Consistent with all polymer counts Chain I； UniProt 1–268 Not recorded MRPL27 × 1 （F1RT79） MRPL28 × 1 （F1RGV8） MRPL47 × 1 MRPL30 × 1 （F1STE5） MRPL32 × 1 （I3LTC4） MRPL33 × 1 MRPL34 × 1 MRPL35 × 1 （F1SVC5） MRPL36 × 1 （I3LDE8） 16S Ribosomal RNA × 1 UNASSIGNED RNA × 1 MRPL2 × 1 （F1RRN2） MRPL3 × 1 （F1RX24） MRPL4 × 1 （F1S3J8） MRPL13 × 1 （F1S286） MRPL14 × 1 （F1RQV7） MRPL15 × 1 （F1RSH0） MRPL16 × 1 （F1RI89） MRPL17 × 1 （F1RMQ4） MRPL18 × 1 （F1SB64） MRPL19 × 1 （I3LNJ0） MRPL20 × 1 （F1RJD0） MRPL21 × 1 （F1RY70） MRPL22 × 1 MRPL23 × 1 MRPL24 × 1 （F1RHJ1） MRPL38 × 1 （F1RW03） MRPL39 × 1 （F1SHP6） MRPL44 × 1 （F1SR64） MRPL45 × 1 MRPL49 × 1 MRPL52 × 1 ICT1 × 1 UNASSIGNED HELICES × 2 THIOREDOXIN FOLD × 1 UNASSIGNED HELICES × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY cryo-EM buffer：20 MM HEPES-KOH PH 7.9, 100 MM KCL, 1 MM DTT, 20 MM MGCL2；pH 7.9；20 MM HEPES-KOH PH 7.9, 100 MM KCL, 1 MM DTT, 20 MM MGCL2 cryo-EM vitrification conditions：Cryogen ETHANE；MANUAL BLOTTING FOLLOWED BY MANUAL PLUNGING INTO LIQUID ETHANE Resolution 4.90 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4CE4 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4V19 Structure of the large subunit of the mammalian mitoribosome, part 1 of 2 Deposited 2014-09-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 28 PDB declaration: 32-meric
Chain I 1–268（268 aa）
Not recorded ZN ZINC ION × 2 MG MAGNESIUM ION × 169 ELECTRON MICROSCOPY
cryo-EM buffer 20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT；pH 7.4；20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；MIXTURE OF LIQUID ETHANE AND PROPANE
Resolution 3.40 Å
5AJ4 Structure of the 55S mammalian mitoribosome. Deposited 2015-02-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 83 PDB declaration: 89-meric
Chain BI 1–268（268 aa）
Not recorded MG MAGNESIUM ION × 351 ZN ZINC ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer 20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT；pH 7.4；20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；MIXTURE OF LIQUID ETHANE AND PROPANE
Resolution 3.80 Å
6GAW Unique features of mammalian mitochondrial translation initiation revealed by cryo-EM. This file contains the complete 55S ribosome. Deposited 2018-04-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 90 PDB declaration: 95-meric
Chain BI 1–268（268 aa）
Not recorded MG MAGNESIUM ION × 326 ZN ZINC ION × 6 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 3 GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 1 NA SODIUM ION × 1 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6GB2 Unique features of mammalian mitochondrial translation initiation revealed by cryo-EM. This file contains the 39S ribosomal subunit. Deposited 2018-04-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 59 PDB declaration: 62-meric
Chain BI 1–268（268 aa）
Not recorded MG MAGNESIUM ION × 216 ZN ZINC ION × 3 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 2 GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 1 NA SODIUM ION × 1 FME N-FORMYLMETHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6YDP 55S mammalian mitochondrial ribosome with mtEFG1 and P site fMet-tRNAMet (POST) Deposited 2020-03-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 89 PDB declaration: 94-meric
Chain BI 1–268（268 aa）
Not recorded MG MAGNESIUM ION × 326 ZN ZINC ION × 6 SPM SPERMINE × 3 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.00 Å
6YDW 55S mammalian mitochondrial ribosome with mtEFG1 and two tRNAMet (TI-POST) Deposited 2020-03-21 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 89 PDB declaration: 95-meric
Chain BI 1–268（268 aa）
Not recorded MG MAGNESIUM ION × 325 ZN ZINC ION × 6 SPM SPERMINE × 3 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.20 Å
7NQH 55S mammalian mitochondrial ribosome with mtRF1a and P-site tRNAMet Deposited 2021-03-01 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 93-meric
Chain BI 1–268（268 aa）
Not recorded MG MAGNESIUM ION × 323 ZN ZINC ION × 6 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.50 Å
7NQL 55S mammalian mitochondrial ribosome with ICT1 and P site tRNAMet Deposited 2021-03-01 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 93-meric
Chain BI 1–268（268 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 335 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.40 Å
7NSH 39S mammalian mitochondrial large ribosomal subunit with mtRRF (post) and mtEFG2 Deposited 2021-03-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 59 PDB declaration: 61-meric
Chain BI 1–268（268 aa）
Not recorded MG MAGNESIUM ION × 221 ZN ZINC ION × 3 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 2 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.20 Å
7NSI 55S mammalian mitochondrial ribosome with mtRRF (pre) and tRNA(P/E) Deposited 2021-03-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 93-meric
Chain BI 1–268（268 aa）
Not recorded MG MAGNESIUM ION × 327 ZN ZINC ION × 6 SPM SPERMINE × 3 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.60 Å
7NSJ 55S mammalian mitochondrial ribosome with tRNA(P/P) and tRNA(E*) Deposited 2021-03-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 87 PDB declaration: 93-meric
Chain BI 1–268（268 aa）
Not recorded MG MAGNESIUM ION × 324 ZN ZINC ION × 6 SPM SPERMINE × 3 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.90 Å
8OIN 55S mammalian mitochondrial ribosome with mtRF1 and P-site tRNA Deposited 2023-03-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 94-meric
Chain BO 1–268（268 aa）
Not recorded MG MAGNESIUM ION × 332 K POTASSIUM ION × 41 PHE PHENYLALANINE × 1 ZN ZINC ION × 3 FES FE2/S2 (INORGANIC) CLUSTER × 3 SPM SPERMINE × 1 SPD SPERMIDINE × 1 FME N-FORMYLMETHIONINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20mM HEPES-KOH pH=7.5, 100mM KCl, 20mM MgCl2, ~1% sucrose (from mtRF1 storage buffer)
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.60 Å
8OIQ 39S mammalian mitochondrial large ribosomal subunit with mtRF1 and P-site tRNA Deposited 2023-03-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 58 PDB declaration: 62-meric
Chain BO 1–268（268 aa）
Not recorded MG MAGNESIUM ION × 205 K POTASSIUM ION × 30 PHE PHENYLALANINE × 1 ZN ZINC ION × 2 FES FE2/S2 (INORGANIC) CLUSTER × 1 FME N-FORMYLMETHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20mM HEPES-KOH pH=7.5, 100mM KCl, 20mM MgCl2, ~1% sucrose (from mtRF1 storage buffer)
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.50 Å

13 other PDB entries and 13 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name I3LR45_PIG
Isoform —
PDB entities 15
Chains and sequence ranges Author chain I； PDBConstruct 1–268； UniProt 1–268

MRPL13

OrganismNot specified

UniProt F1S286

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 36 RNA 2 PDB declaration: 38-meric（38） Consistent with all polymer counts Chain N； UniProt 1–178 Not recorded MRPL27 × 1 （F1RT79） MRPL28 × 1 （F1RGV8） MRPL47 × 1 MRPL30 × 1 （F1STE5） MRPL32 × 1 （I3LTC4） MRPL33 × 1 MRPL34 × 1 MRPL35 × 1 （F1SVC5） MRPL36 × 1 （I3LDE8） 16S Ribosomal RNA × 1 UNASSIGNED RNA × 1 MRPL2 × 1 （F1RRN2） MRPL3 × 1 （F1RX24） MRPL4 × 1 （F1S3J8） MRPL9 × 1 （I3LR45） MRPL14 × 1 （F1RQV7） MRPL15 × 1 （F1RSH0） MRPL16 × 1 （F1RI89） MRPL17 × 1 （F1RMQ4） MRPL18 × 1 （F1SB64） MRPL19 × 1 （I3LNJ0） MRPL20 × 1 （F1RJD0） MRPL21 × 1 （F1RY70） MRPL22 × 1 MRPL23 × 1 MRPL24 × 1 （F1RHJ1） MRPL38 × 1 （F1RW03） MRPL39 × 1 （F1SHP6） MRPL44 × 1 （F1SR64） MRPL45 × 1 MRPL49 × 1 MRPL52 × 1 ICT1 × 1 UNASSIGNED HELICES × 2 THIOREDOXIN FOLD × 1 UNASSIGNED HELICES × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY cryo-EM buffer：20 MM HEPES-KOH PH 7.9, 100 MM KCL, 1 MM DTT, 20 MM MGCL2；pH 7.9；20 MM HEPES-KOH PH 7.9, 100 MM KCL, 1 MM DTT, 20 MM MGCL2 cryo-EM vitrification conditions：Cryogen ETHANE；MANUAL BLOTTING FOLLOWED BY MANUAL PLUNGING INTO LIQUID ETHANE Resolution 4.90 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4CE4 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4V19 Structure of the large subunit of the mammalian mitoribosome, part 1 of 2 Deposited 2014-09-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 28 PDB declaration: 32-meric
Chain N 1–178（178 aa）
Not recorded ZN ZINC ION × 2 MG MAGNESIUM ION × 169 ELECTRON MICROSCOPY
cryo-EM buffer 20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT；pH 7.4；20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；MIXTURE OF LIQUID ETHANE AND PROPANE
Resolution 3.40 Å
5AJ4 Structure of the 55S mammalian mitoribosome. Deposited 2015-02-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 83 PDB declaration: 89-meric
Chain BN 1–178（178 aa）
Not recorded MG MAGNESIUM ION × 351 ZN ZINC ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer 20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT；pH 7.4；20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；MIXTURE OF LIQUID ETHANE AND PROPANE
Resolution 3.80 Å

2 other PDB entries and 2 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name F1S286_PIG
Isoform —
PDB entities 16
Chains and sequence ranges Author chain N； PDBConstruct 1–178； UniProt 1–178

MRPL14

OrganismNot specified

UniProt F1RQV7

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 36 RNA 2 PDB declaration: 38-meric（38） Consistent with all polymer counts Chain O； UniProt 1–145 Not recorded MRPL27 × 1 （F1RT79） MRPL28 × 1 （F1RGV8） MRPL47 × 1 MRPL30 × 1 （F1STE5） MRPL32 × 1 （I3LTC4） MRPL33 × 1 MRPL34 × 1 MRPL35 × 1 （F1SVC5） MRPL36 × 1 （I3LDE8） 16S Ribosomal RNA × 1 UNASSIGNED RNA × 1 MRPL2 × 1 （F1RRN2） MRPL3 × 1 （F1RX24） MRPL4 × 1 （F1S3J8） MRPL9 × 1 （I3LR45） MRPL13 × 1 （F1S286） MRPL15 × 1 （F1RSH0） MRPL16 × 1 （F1RI89） MRPL17 × 1 （F1RMQ4） MRPL18 × 1 （F1SB64） MRPL19 × 1 （I3LNJ0） MRPL20 × 1 （F1RJD0） MRPL21 × 1 （F1RY70） MRPL22 × 1 MRPL23 × 1 MRPL24 × 1 （F1RHJ1） MRPL38 × 1 （F1RW03） MRPL39 × 1 （F1SHP6） MRPL44 × 1 （F1SR64） MRPL45 × 1 MRPL49 × 1 MRPL52 × 1 ICT1 × 1 UNASSIGNED HELICES × 2 THIOREDOXIN FOLD × 1 UNASSIGNED HELICES × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY cryo-EM buffer：20 MM HEPES-KOH PH 7.9, 100 MM KCL, 1 MM DTT, 20 MM MGCL2；pH 7.9；20 MM HEPES-KOH PH 7.9, 100 MM KCL, 1 MM DTT, 20 MM MGCL2 cryo-EM vitrification conditions：Cryogen ETHANE；MANUAL BLOTTING FOLLOWED BY MANUAL PLUNGING INTO LIQUID ETHANE Resolution 4.90 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4CE4 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4V19 Structure of the large subunit of the mammalian mitoribosome, part 1 of 2 Deposited 2014-09-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 28 PDB declaration: 32-meric
Chain O 1–145（145 aa）
Not recorded ZN ZINC ION × 2 MG MAGNESIUM ION × 169 ELECTRON MICROSCOPY
cryo-EM buffer 20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT；pH 7.4；20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；MIXTURE OF LIQUID ETHANE AND PROPANE
Resolution 3.40 Å
5AJ4 Structure of the 55S mammalian mitoribosome. Deposited 2015-02-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 83 PDB declaration: 89-meric
Chain BO 1–145（145 aa）
Not recorded MG MAGNESIUM ION × 351 ZN ZINC ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer 20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT；pH 7.4；20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；MIXTURE OF LIQUID ETHANE AND PROPANE
Resolution 3.80 Å

2 other PDB entries and 2 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name F1RQV7_PIG
Isoform —
PDB entities 17
Chains and sequence ranges Author chain O； PDBConstruct 1–145； UniProt 1–145

MRPL15

OrganismNot specified

UniProt F1RSH0

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 36 RNA 2 PDB declaration: 38-meric（38） Consistent with all polymer counts Chain P； UniProt 22–296 Not recorded MRPL27 × 1 （F1RT79） MRPL28 × 1 （F1RGV8） MRPL47 × 1 MRPL30 × 1 （F1STE5） MRPL32 × 1 （I3LTC4） MRPL33 × 1 MRPL34 × 1 MRPL35 × 1 （F1SVC5） MRPL36 × 1 （I3LDE8） 16S Ribosomal RNA × 1 UNASSIGNED RNA × 1 MRPL2 × 1 （F1RRN2） MRPL3 × 1 （F1RX24） MRPL4 × 1 （F1S3J8） MRPL9 × 1 （I3LR45） MRPL13 × 1 （F1S286） MRPL14 × 1 （F1RQV7） MRPL16 × 1 （F1RI89） MRPL17 × 1 （F1RMQ4） MRPL18 × 1 （F1SB64） MRPL19 × 1 （I3LNJ0） MRPL20 × 1 （F1RJD0） MRPL21 × 1 （F1RY70） MRPL22 × 1 MRPL23 × 1 MRPL24 × 1 （F1RHJ1） MRPL38 × 1 （F1RW03） MRPL39 × 1 （F1SHP6） MRPL44 × 1 （F1SR64） MRPL45 × 1 MRPL49 × 1 MRPL52 × 1 ICT1 × 1 UNASSIGNED HELICES × 2 THIOREDOXIN FOLD × 1 UNASSIGNED HELICES × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY cryo-EM buffer：20 MM HEPES-KOH PH 7.9, 100 MM KCL, 1 MM DTT, 20 MM MGCL2；pH 7.9；20 MM HEPES-KOH PH 7.9, 100 MM KCL, 1 MM DTT, 20 MM MGCL2 cryo-EM vitrification conditions：Cryogen ETHANE；MANUAL BLOTTING FOLLOWED BY MANUAL PLUNGING INTO LIQUID ETHANE Resolution 4.90 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4CE4 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4V19 Structure of the large subunit of the mammalian mitoribosome, part 1 of 2 Deposited 2014-09-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 28 PDB declaration: 32-meric
Chain P 1–296（296 aa）
Not recorded ZN ZINC ION × 2 MG MAGNESIUM ION × 169 ELECTRON MICROSCOPY
cryo-EM buffer 20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT；pH 7.4；20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；MIXTURE OF LIQUID ETHANE AND PROPANE
Resolution 3.40 Å
5AJ4 Structure of the 55S mammalian mitoribosome. Deposited 2015-02-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 83 PDB declaration: 89-meric
Chain BP 1–296（296 aa）
Not recorded MG MAGNESIUM ION × 351 ZN ZINC ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer 20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT；pH 7.4；20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；MIXTURE OF LIQUID ETHANE AND PROPANE
Resolution 3.80 Å
6GAW Unique features of mammalian mitochondrial translation initiation revealed by cryo-EM. This file contains the complete 55S ribosome. Deposited 2018-04-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 90 PDB declaration: 95-meric
Chain BP 1–296（296 aa）
Not recorded MG MAGNESIUM ION × 326 ZN ZINC ION × 6 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 3 GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 1 NA SODIUM ION × 1 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6GB2 Unique features of mammalian mitochondrial translation initiation revealed by cryo-EM. This file contains the 39S ribosomal subunit. Deposited 2018-04-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 59 PDB declaration: 62-meric
Chain BP 1–296（296 aa）
Not recorded MG MAGNESIUM ION × 216 ZN ZINC ION × 3 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 2 GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 1 NA SODIUM ION × 1 FME N-FORMYLMETHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6YDP 55S mammalian mitochondrial ribosome with mtEFG1 and P site fMet-tRNAMet (POST) Deposited 2020-03-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 89 PDB declaration: 94-meric
Chain BP 1–296（296 aa）
Not recorded MG MAGNESIUM ION × 326 ZN ZINC ION × 6 SPM SPERMINE × 3 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.00 Å
6YDW 55S mammalian mitochondrial ribosome with mtEFG1 and two tRNAMet (TI-POST) Deposited 2020-03-21 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 89 PDB declaration: 95-meric
Chain BP 1–296（296 aa）
Not recorded MG MAGNESIUM ION × 325 ZN ZINC ION × 6 SPM SPERMINE × 3 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.20 Å
7NQH 55S mammalian mitochondrial ribosome with mtRF1a and P-site tRNAMet Deposited 2021-03-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 93-meric
Chain BP 1–296（296 aa）
Not recorded MG MAGNESIUM ION × 323 ZN ZINC ION × 6 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.50 Å
7NQL 55S mammalian mitochondrial ribosome with ICT1 and P site tRNAMet Deposited 2021-03-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 93-meric
Chain BP 1–296（296 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 335 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.40 Å
7NSH 39S mammalian mitochondrial large ribosomal subunit with mtRRF (post) and mtEFG2 Deposited 2021-03-07 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 59 PDB declaration: 61-meric
Chain BP 1–296（296 aa）
Not recorded MG MAGNESIUM ION × 221 ZN ZINC ION × 3 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 2 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.20 Å
7NSJ 55S mammalian mitochondrial ribosome with tRNA(P/P) and tRNA(E*) Deposited 2021-03-07 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 87 PDB declaration: 93-meric
Chain BP 1–296（296 aa）
Not recorded MG MAGNESIUM ION × 324 ZN ZINC ION × 6 SPM SPERMINE × 3 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.90 Å

10 other PDB entries and 10 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name F1RSH0_PIG
Isoform —
PDB entities 18
Chains and sequence ranges Author chain P； PDBConstruct 14–288； UniProt 22–296

MRPL16

OrganismNot specified

UniProt F1RI89

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 36 RNA 2 PDB declaration: 38-meric（38） Consistent with all polymer counts Chain Q； UniProt 1–191 Not recorded MRPL27 × 1 （F1RT79） MRPL28 × 1 （F1RGV8） MRPL47 × 1 MRPL30 × 1 （F1STE5） MRPL32 × 1 （I3LTC4） MRPL33 × 1 MRPL34 × 1 MRPL35 × 1 （F1SVC5） MRPL36 × 1 （I3LDE8） 16S Ribosomal RNA × 1 UNASSIGNED RNA × 1 MRPL2 × 1 （F1RRN2） MRPL3 × 1 （F1RX24） MRPL4 × 1 （F1S3J8） MRPL9 × 1 （I3LR45） MRPL13 × 1 （F1S286） MRPL14 × 1 （F1RQV7） MRPL15 × 1 （F1RSH0） MRPL17 × 1 （F1RMQ4） MRPL18 × 1 （F1SB64） MRPL19 × 1 （I3LNJ0） MRPL20 × 1 （F1RJD0） MRPL21 × 1 （F1RY70） MRPL22 × 1 MRPL23 × 1 MRPL24 × 1 （F1RHJ1） MRPL38 × 1 （F1RW03） MRPL39 × 1 （F1SHP6） MRPL44 × 1 （F1SR64） MRPL45 × 1 MRPL49 × 1 MRPL52 × 1 ICT1 × 1 UNASSIGNED HELICES × 2 THIOREDOXIN FOLD × 1 UNASSIGNED HELICES × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY cryo-EM buffer：20 MM HEPES-KOH PH 7.9, 100 MM KCL, 1 MM DTT, 20 MM MGCL2；pH 7.9；20 MM HEPES-KOH PH 7.9, 100 MM KCL, 1 MM DTT, 20 MM MGCL2 cryo-EM vitrification conditions：Cryogen ETHANE；MANUAL BLOTTING FOLLOWED BY MANUAL PLUNGING INTO LIQUID ETHANE Resolution 4.90 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4CE4 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4V19 Structure of the large subunit of the mammalian mitoribosome, part 1 of 2 Deposited 2014-09-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 28 PDB declaration: 32-meric
Chain Q 1–251（251 aa）
Not recorded ZN ZINC ION × 2 MG MAGNESIUM ION × 169 ELECTRON MICROSCOPY
cryo-EM buffer 20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT；pH 7.4；20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；MIXTURE OF LIQUID ETHANE AND PROPANE
Resolution 3.40 Å
5AJ4 Structure of the 55S mammalian mitoribosome. Deposited 2015-02-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 83 PDB declaration: 89-meric
Chain BQ 1–251（251 aa）
Not recorded MG MAGNESIUM ION × 351 ZN ZINC ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer 20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT；pH 7.4；20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；MIXTURE OF LIQUID ETHANE AND PROPANE
Resolution 3.80 Å
6GAW Unique features of mammalian mitochondrial translation initiation revealed by cryo-EM. This file contains the complete 55S ribosome. Deposited 2018-04-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 90 PDB declaration: 95-meric
Chain BQ 1–251（251 aa）
Not recorded MG MAGNESIUM ION × 326 ZN ZINC ION × 6 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 3 GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 1 NA SODIUM ION × 1 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6GB2 Unique features of mammalian mitochondrial translation initiation revealed by cryo-EM. This file contains the 39S ribosomal subunit. Deposited 2018-04-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 59 PDB declaration: 62-meric
Chain BQ 1–251（251 aa）
Not recorded MG MAGNESIUM ION × 216 ZN ZINC ION × 3 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 2 GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 1 NA SODIUM ION × 1 FME N-FORMYLMETHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6YDP 55S mammalian mitochondrial ribosome with mtEFG1 and P site fMet-tRNAMet (POST) Deposited 2020-03-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 89 PDB declaration: 94-meric
Chain BQ 1–251（251 aa）
Not recorded MG MAGNESIUM ION × 326 ZN ZINC ION × 6 SPM SPERMINE × 3 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.00 Å
6YDW 55S mammalian mitochondrial ribosome with mtEFG1 and two tRNAMet (TI-POST) Deposited 2020-03-21 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 89 PDB declaration: 95-meric
Chain BQ 1–251（251 aa）
Not recorded MG MAGNESIUM ION × 325 ZN ZINC ION × 6 SPM SPERMINE × 3 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.20 Å
7NQH 55S mammalian mitochondrial ribosome with mtRF1a and P-site tRNAMet Deposited 2021-03-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 93-meric
Chain BQ 1–251（251 aa）
Not recorded MG MAGNESIUM ION × 323 ZN ZINC ION × 6 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.50 Å
7NQL 55S mammalian mitochondrial ribosome with ICT1 and P site tRNAMet Deposited 2021-03-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 93-meric
Chain BQ 1–251（251 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 335 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.40 Å
7NSH 39S mammalian mitochondrial large ribosomal subunit with mtRRF (post) and mtEFG2 Deposited 2021-03-07 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 59 PDB declaration: 61-meric
Chain BQ 1–251（251 aa）
Not recorded MG MAGNESIUM ION × 221 ZN ZINC ION × 3 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 2 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.20 Å
7NSJ 55S mammalian mitochondrial ribosome with tRNA(P/P) and tRNA(E*) Deposited 2021-03-07 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 87 PDB declaration: 93-meric
Chain BQ 1–251（251 aa）
Not recorded MG MAGNESIUM ION × 324 ZN ZINC ION × 6 SPM SPERMINE × 3 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.90 Å
8OIN 55S mammalian mitochondrial ribosome with mtRF1 and P-site tRNA Deposited 2023-03-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 94-meric
Chain BU 1–251（251 aa）
Not recorded MG MAGNESIUM ION × 332 K POTASSIUM ION × 41 PHE PHENYLALANINE × 1 ZN ZINC ION × 3 FES FE2/S2 (INORGANIC) CLUSTER × 3 SPM SPERMINE × 1 SPD SPERMIDINE × 1 FME N-FORMYLMETHIONINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20mM HEPES-KOH pH=7.5, 100mM KCl, 20mM MgCl2, ~1% sucrose (from mtRF1 storage buffer)
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.60 Å
8OIQ 39S mammalian mitochondrial large ribosomal subunit with mtRF1 and P-site tRNA Deposited 2023-03-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 58 PDB declaration: 62-meric
Chain BU 1–251（251 aa）
Not recorded MG MAGNESIUM ION × 205 K POTASSIUM ION × 30 PHE PHENYLALANINE × 1 ZN ZINC ION × 2 FES FE2/S2 (INORGANIC) CLUSTER × 1 FME N-FORMYLMETHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20mM HEPES-KOH pH=7.5, 100mM KCl, 20mM MgCl2, ~1% sucrose (from mtRF1 storage buffer)
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.50 Å

12 other PDB entries and 12 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name F1RI89_PIG
Isoform —
PDB entities 19
Chains and sequence ranges Author chain Q； PDBConstruct 1–191； UniProt 1–191

MRPL17

OrganismNot specified

UniProt F1RMQ4

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 36 RNA 2 PDB declaration: 38-meric（38） Consistent with all polymer counts Chain R； UniProt 1–169 Not recorded MRPL27 × 1 （F1RT79） MRPL28 × 1 （F1RGV8） MRPL47 × 1 MRPL30 × 1 （F1STE5） MRPL32 × 1 （I3LTC4） MRPL33 × 1 MRPL34 × 1 MRPL35 × 1 （F1SVC5） MRPL36 × 1 （I3LDE8） 16S Ribosomal RNA × 1 UNASSIGNED RNA × 1 MRPL2 × 1 （F1RRN2） MRPL3 × 1 （F1RX24） MRPL4 × 1 （F1S3J8） MRPL9 × 1 （I3LR45） MRPL13 × 1 （F1S286） MRPL14 × 1 （F1RQV7） MRPL15 × 1 （F1RSH0） MRPL16 × 1 （F1RI89） MRPL18 × 1 （F1SB64） MRPL19 × 1 （I3LNJ0） MRPL20 × 1 （F1RJD0） MRPL21 × 1 （F1RY70） MRPL22 × 1 MRPL23 × 1 MRPL24 × 1 （F1RHJ1） MRPL38 × 1 （F1RW03） MRPL39 × 1 （F1SHP6） MRPL44 × 1 （F1SR64） MRPL45 × 1 MRPL49 × 1 MRPL52 × 1 ICT1 × 1 UNASSIGNED HELICES × 2 THIOREDOXIN FOLD × 1 UNASSIGNED HELICES × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY cryo-EM buffer：20 MM HEPES-KOH PH 7.9, 100 MM KCL, 1 MM DTT, 20 MM MGCL2；pH 7.9；20 MM HEPES-KOH PH 7.9, 100 MM KCL, 1 MM DTT, 20 MM MGCL2 cryo-EM vitrification conditions：Cryogen ETHANE；MANUAL BLOTTING FOLLOWED BY MANUAL PLUNGING INTO LIQUID ETHANE Resolution 4.90 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4CE4 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4V19 Structure of the large subunit of the mammalian mitoribosome, part 1 of 2 Deposited 2014-09-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 28 PDB declaration: 32-meric
Chain R 1–169（169 aa）
Not recorded ZN ZINC ION × 2 MG MAGNESIUM ION × 169 ELECTRON MICROSCOPY
cryo-EM buffer 20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT；pH 7.4；20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；MIXTURE OF LIQUID ETHANE AND PROPANE
Resolution 3.40 Å
5AJ4 Structure of the 55S mammalian mitoribosome. Deposited 2015-02-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 83 PDB declaration: 89-meric
Chain BR 1–169（169 aa）
Not recorded MG MAGNESIUM ION × 351 ZN ZINC ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer 20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT；pH 7.4；20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；MIXTURE OF LIQUID ETHANE AND PROPANE
Resolution 3.80 Å
6GAW Unique features of mammalian mitochondrial translation initiation revealed by cryo-EM. This file contains the complete 55S ribosome. Deposited 2018-04-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 90 PDB declaration: 95-meric
Chain BR 1–169（169 aa）
Not recorded MG MAGNESIUM ION × 326 ZN ZINC ION × 6 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 3 GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 1 NA SODIUM ION × 1 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6GB2 Unique features of mammalian mitochondrial translation initiation revealed by cryo-EM. This file contains the 39S ribosomal subunit. Deposited 2018-04-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 59 PDB declaration: 62-meric
Chain BR 1–169（169 aa）
Not recorded MG MAGNESIUM ION × 216 ZN ZINC ION × 3 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 2 GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 1 NA SODIUM ION × 1 FME N-FORMYLMETHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6YDP 55S mammalian mitochondrial ribosome with mtEFG1 and P site fMet-tRNAMet (POST) Deposited 2020-03-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 89 PDB declaration: 94-meric
Chain BR 1–169（169 aa）
Not recorded MG MAGNESIUM ION × 326 ZN ZINC ION × 6 SPM SPERMINE × 3 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.00 Å
6YDW 55S mammalian mitochondrial ribosome with mtEFG1 and two tRNAMet (TI-POST) Deposited 2020-03-21 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 89 PDB declaration: 95-meric
Chain BR 1–169（169 aa）
Not recorded MG MAGNESIUM ION × 325 ZN ZINC ION × 6 SPM SPERMINE × 3 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.20 Å
7NQH 55S mammalian mitochondrial ribosome with mtRF1a and P-site tRNAMet Deposited 2021-03-01 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 93-meric
Chain BR 1–169（169 aa）
Not recorded MG MAGNESIUM ION × 323 ZN ZINC ION × 6 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.50 Å
7NQL 55S mammalian mitochondrial ribosome with ICT1 and P site tRNAMet Deposited 2021-03-01 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 93-meric
Chain BR 1–169（169 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 335 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.40 Å
7NSH 39S mammalian mitochondrial large ribosomal subunit with mtRRF (post) and mtEFG2 Deposited 2021-03-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 59 PDB declaration: 61-meric
Chain BR 1–169（169 aa）
Not recorded MG MAGNESIUM ION × 221 ZN ZINC ION × 3 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 2 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.20 Å
7NSI 55S mammalian mitochondrial ribosome with mtRRF (pre) and tRNA(P/E) Deposited 2021-03-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 93-meric
Chain BR 1–169（169 aa）
Not recorded MG MAGNESIUM ION × 327 ZN ZINC ION × 6 SPM SPERMINE × 3 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.60 Å
7NSJ 55S mammalian mitochondrial ribosome with tRNA(P/P) and tRNA(E*) Deposited 2021-03-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 87 PDB declaration: 93-meric
Chain BR 1–169（169 aa）
Not recorded MG MAGNESIUM ION × 324 ZN ZINC ION × 6 SPM SPERMINE × 3 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.90 Å
8OIN 55S mammalian mitochondrial ribosome with mtRF1 and P-site tRNA Deposited 2023-03-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 94-meric
Chain BV 1–169（169 aa）
Not recorded MG MAGNESIUM ION × 332 K POTASSIUM ION × 41 PHE PHENYLALANINE × 1 ZN ZINC ION × 3 FES FE2/S2 (INORGANIC) CLUSTER × 3 SPM SPERMINE × 1 SPD SPERMIDINE × 1 FME N-FORMYLMETHIONINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20mM HEPES-KOH pH=7.5, 100mM KCl, 20mM MgCl2, ~1% sucrose (from mtRF1 storage buffer)
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.60 Å
8OIQ 39S mammalian mitochondrial large ribosomal subunit with mtRF1 and P-site tRNA Deposited 2023-03-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 58 PDB declaration: 62-meric
Chain BV 1–169（169 aa）
Not recorded MG MAGNESIUM ION × 205 K POTASSIUM ION × 30 PHE PHENYLALANINE × 1 ZN ZINC ION × 2 FES FE2/S2 (INORGANIC) CLUSTER × 1 FME N-FORMYLMETHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20mM HEPES-KOH pH=7.5, 100mM KCl, 20mM MgCl2, ~1% sucrose (from mtRF1 storage buffer)
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.50 Å

13 other PDB entries and 13 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name F1RMQ4_PIG
Isoform —
PDB entities 20
Chains and sequence ranges Author chain R； PDBConstruct 1–169； UniProt 1–169

MRPL18

OrganismNot specified

UniProt F1SB64

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 36 RNA 2 PDB declaration: 38-meric（38） Consistent with all polymer counts Chain S； UniProt 1–180 Not recorded MRPL27 × 1 （F1RT79） MRPL28 × 1 （F1RGV8） MRPL47 × 1 MRPL30 × 1 （F1STE5） MRPL32 × 1 （I3LTC4） MRPL33 × 1 MRPL34 × 1 MRPL35 × 1 （F1SVC5） MRPL36 × 1 （I3LDE8） 16S Ribosomal RNA × 1 UNASSIGNED RNA × 1 MRPL2 × 1 （F1RRN2） MRPL3 × 1 （F1RX24） MRPL4 × 1 （F1S3J8） MRPL9 × 1 （I3LR45） MRPL13 × 1 （F1S286） MRPL14 × 1 （F1RQV7） MRPL15 × 1 （F1RSH0） MRPL16 × 1 （F1RI89） MRPL17 × 1 （F1RMQ4） MRPL19 × 1 （I3LNJ0） MRPL20 × 1 （F1RJD0） MRPL21 × 1 （F1RY70） MRPL22 × 1 MRPL23 × 1 MRPL24 × 1 （F1RHJ1） MRPL38 × 1 （F1RW03） MRPL39 × 1 （F1SHP6） MRPL44 × 1 （F1SR64） MRPL45 × 1 MRPL49 × 1 MRPL52 × 1 ICT1 × 1 UNASSIGNED HELICES × 2 THIOREDOXIN FOLD × 1 UNASSIGNED HELICES × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY cryo-EM buffer：20 MM HEPES-KOH PH 7.9, 100 MM KCL, 1 MM DTT, 20 MM MGCL2；pH 7.9；20 MM HEPES-KOH PH 7.9, 100 MM KCL, 1 MM DTT, 20 MM MGCL2 cryo-EM vitrification conditions：Cryogen ETHANE；MANUAL BLOTTING FOLLOWED BY MANUAL PLUNGING INTO LIQUID ETHANE Resolution 4.90 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4CE4 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4V19 Structure of the large subunit of the mammalian mitoribosome, part 1 of 2 Deposited 2014-09-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 28 PDB declaration: 32-meric
Chain S 1–180（180 aa）
Not recorded ZN ZINC ION × 2 MG MAGNESIUM ION × 169 ELECTRON MICROSCOPY
cryo-EM buffer 20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT；pH 7.4；20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；MIXTURE OF LIQUID ETHANE AND PROPANE
Resolution 3.40 Å
5AJ4 Structure of the 55S mammalian mitoribosome. Deposited 2015-02-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 83 PDB declaration: 89-meric
Chain BS 1–180（180 aa）
Not recorded MG MAGNESIUM ION × 351 ZN ZINC ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer 20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT；pH 7.4；20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；MIXTURE OF LIQUID ETHANE AND PROPANE
Resolution 3.80 Å

2 other PDB entries and 2 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name F1SB64_PIG
Isoform —
PDB entities 21
Chains and sequence ranges Author chain S； PDBConstruct 1–180； UniProt 1–180

MRPL19

OrganismNot specified

UniProt I3LNJ0

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 36 RNA 2 PDB declaration: 38-meric（38） Consistent with all polymer counts Chain T； UniProt 1–292 Not recorded MRPL27 × 1 （F1RT79） MRPL28 × 1 （F1RGV8） MRPL47 × 1 MRPL30 × 1 （F1STE5） MRPL32 × 1 （I3LTC4） MRPL33 × 1 MRPL34 × 1 MRPL35 × 1 （F1SVC5） MRPL36 × 1 （I3LDE8） 16S Ribosomal RNA × 1 UNASSIGNED RNA × 1 MRPL2 × 1 （F1RRN2） MRPL3 × 1 （F1RX24） MRPL4 × 1 （F1S3J8） MRPL9 × 1 （I3LR45） MRPL13 × 1 （F1S286） MRPL14 × 1 （F1RQV7） MRPL15 × 1 （F1RSH0） MRPL16 × 1 （F1RI89） MRPL17 × 1 （F1RMQ4） MRPL18 × 1 （F1SB64） MRPL20 × 1 （F1RJD0） MRPL21 × 1 （F1RY70） MRPL22 × 1 MRPL23 × 1 MRPL24 × 1 （F1RHJ1） MRPL38 × 1 （F1RW03） MRPL39 × 1 （F1SHP6） MRPL44 × 1 （F1SR64） MRPL45 × 1 MRPL49 × 1 MRPL52 × 1 ICT1 × 1 UNASSIGNED HELICES × 2 THIOREDOXIN FOLD × 1 UNASSIGNED HELICES × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY cryo-EM buffer：20 MM HEPES-KOH PH 7.9, 100 MM KCL, 1 MM DTT, 20 MM MGCL2；pH 7.9；20 MM HEPES-KOH PH 7.9, 100 MM KCL, 1 MM DTT, 20 MM MGCL2 cryo-EM vitrification conditions：Cryogen ETHANE；MANUAL BLOTTING FOLLOWED BY MANUAL PLUNGING INTO LIQUID ETHANE Resolution 4.90 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4CE4 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4V19 Structure of the large subunit of the mammalian mitoribosome, part 1 of 2 Deposited 2014-09-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 28 PDB declaration: 32-meric
Chain T 1–292（292 aa）
Not recorded ZN ZINC ION × 2 MG MAGNESIUM ION × 169 ELECTRON MICROSCOPY
cryo-EM buffer 20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT；pH 7.4；20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；MIXTURE OF LIQUID ETHANE AND PROPANE
Resolution 3.40 Å
5AJ4 Structure of the 55S mammalian mitoribosome. Deposited 2015-02-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 83 PDB declaration: 89-meric
Chain BT 1–292（292 aa）
Not recorded MG MAGNESIUM ION × 351 ZN ZINC ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer 20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT；pH 7.4；20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；MIXTURE OF LIQUID ETHANE AND PROPANE
Resolution 3.80 Å
6GAW Unique features of mammalian mitochondrial translation initiation revealed by cryo-EM. This file contains the complete 55S ribosome. Deposited 2018-04-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 90 PDB declaration: 95-meric
Chain BT 12–303（292 aa）
Not recorded MG MAGNESIUM ION × 326 ZN ZINC ION × 6 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 3 GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 1 NA SODIUM ION × 1 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6GAZ Unique features of mammalian mitochondrial translation initiation revealed by cryo-EM. This file contains the 28S ribosomal subunit. Deposited 2018-04-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 36-meric
Chain BT 12–303（292 aa）
Not recorded GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 1 MG MAGNESIUM ION × 111 NA SODIUM ION × 1 SPM SPERMINE × 1 ZN ZINC ION × 3 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
6YDP 55S mammalian mitochondrial ribosome with mtEFG1 and P site fMet-tRNAMet (POST) Deposited 2020-03-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 89 PDB declaration: 94-meric
Chain BT 12–303（292 aa）
Not recorded MG MAGNESIUM ION × 326 ZN ZINC ION × 6 SPM SPERMINE × 3 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.00 Å
6YDW 55S mammalian mitochondrial ribosome with mtEFG1 and two tRNAMet (TI-POST) Deposited 2020-03-21 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 89 PDB declaration: 95-meric
Chain BT 12–303（292 aa）
Not recorded MG MAGNESIUM ION × 325 ZN ZINC ION × 6 SPM SPERMINE × 3 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.20 Å
7NQH 55S mammalian mitochondrial ribosome with mtRF1a and P-site tRNAMet Deposited 2021-03-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 93-meric
Chain BT 12–303（292 aa）
Not recorded MG MAGNESIUM ION × 323 ZN ZINC ION × 6 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.50 Å
7NQL 55S mammalian mitochondrial ribosome with ICT1 and P site tRNAMet Deposited 2021-03-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 93-meric
Chain BT 12–303（292 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 335 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.40 Å
7NSH 39S mammalian mitochondrial large ribosomal subunit with mtRRF (post) and mtEFG2 Deposited 2021-03-07 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 59 PDB declaration: 61-meric
Chain BT 12–303（292 aa）
Not recorded MG MAGNESIUM ION × 221 ZN ZINC ION × 3 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 2 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.20 Å
7NSI 55S mammalian mitochondrial ribosome with mtRRF (pre) and tRNA(P/E) Deposited 2021-03-07 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 93-meric
Chain BT 12–303（292 aa）
Not recorded MG MAGNESIUM ION × 327 ZN ZINC ION × 6 SPM SPERMINE × 3 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.60 Å
7NSJ 55S mammalian mitochondrial ribosome with tRNA(P/P) and tRNA(E*) Deposited 2021-03-07 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 87 PDB declaration: 93-meric
Chain BT 12–303（292 aa）
Not recorded MG MAGNESIUM ION × 324 ZN ZINC ION × 6 SPM SPERMINE × 3 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.90 Å
8OIN 55S mammalian mitochondrial ribosome with mtRF1 and P-site tRNA Deposited 2023-03-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 94-meric
Chain BX 1–303（303 aa）
Not recorded MG MAGNESIUM ION × 332 K POTASSIUM ION × 41 PHE PHENYLALANINE × 1 ZN ZINC ION × 3 FES FE2/S2 (INORGANIC) CLUSTER × 3 SPM SPERMINE × 1 SPD SPERMIDINE × 1 FME N-FORMYLMETHIONINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20mM HEPES-KOH pH=7.5, 100mM KCl, 20mM MgCl2, ~1% sucrose (from mtRF1 storage buffer)
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.60 Å
8OIP 28S mammalian mitochondrial small ribosomal subunit with mtRF1 and P-site tRNA Deposited 2023-03-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 36-meric
Chain BX 1–303（303 aa）
Not recorded MG MAGNESIUM ION × 127 SPM SPERMINE × 1 SPD SPERMIDINE × 1 K POTASSIUM ION × 11 ZN ZINC ION × 1 FS2 FE-S-O HYBRID CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20mM HEPES-KOH pH=7.5, 100mM KCl, 20mM MgCl2, ~1% sucrose (from mtRF1 storage buffer)
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.60 Å
8OIQ 39S mammalian mitochondrial large ribosomal subunit with mtRF1 and P-site tRNA Deposited 2023-03-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 58 PDB declaration: 62-meric
Chain BX 1–303（303 aa）
Not recorded MG MAGNESIUM ION × 205 K POTASSIUM ION × 30 PHE PHENYLALANINE × 1 ZN ZINC ION × 2 FES FE2/S2 (INORGANIC) CLUSTER × 1 FME N-FORMYLMETHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20mM HEPES-KOH pH=7.5, 100mM KCl, 20mM MgCl2, ~1% sucrose (from mtRF1 storage buffer)
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.50 Å

14 other PDB entries and 14 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name I3LNJ0_PIG
Isoform —
PDB entities 22
Chains and sequence ranges Author chain T； PDBConstruct 1–292； UniProt 1–292

MRPL20

OrganismNot specified

UniProt F1RJD0

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 36 RNA 2 PDB declaration: 38-meric（38） Consistent with all polymer counts Chain U； UniProt 1–124 Not recorded MRPL27 × 1 （F1RT79） MRPL28 × 1 （F1RGV8） MRPL47 × 1 MRPL30 × 1 （F1STE5） MRPL32 × 1 （I3LTC4） MRPL33 × 1 MRPL34 × 1 MRPL35 × 1 （F1SVC5） MRPL36 × 1 （I3LDE8） 16S Ribosomal RNA × 1 UNASSIGNED RNA × 1 MRPL2 × 1 （F1RRN2） MRPL3 × 1 （F1RX24） MRPL4 × 1 （F1S3J8） MRPL9 × 1 （I3LR45） MRPL13 × 1 （F1S286） MRPL14 × 1 （F1RQV7） MRPL15 × 1 （F1RSH0） MRPL16 × 1 （F1RI89） MRPL17 × 1 （F1RMQ4） MRPL18 × 1 （F1SB64） MRPL19 × 1 （I3LNJ0） MRPL21 × 1 （F1RY70） MRPL22 × 1 MRPL23 × 1 MRPL24 × 1 （F1RHJ1） MRPL38 × 1 （F1RW03） MRPL39 × 1 （F1SHP6） MRPL44 × 1 （F1SR64） MRPL45 × 1 MRPL49 × 1 MRPL52 × 1 ICT1 × 1 UNASSIGNED HELICES × 2 THIOREDOXIN FOLD × 1 UNASSIGNED HELICES × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY cryo-EM buffer：20 MM HEPES-KOH PH 7.9, 100 MM KCL, 1 MM DTT, 20 MM MGCL2；pH 7.9；20 MM HEPES-KOH PH 7.9, 100 MM KCL, 1 MM DTT, 20 MM MGCL2 cryo-EM vitrification conditions：Cryogen ETHANE；MANUAL BLOTTING FOLLOWED BY MANUAL PLUNGING INTO LIQUID ETHANE Resolution 4.90 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4CE4 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4V19 Structure of the large subunit of the mammalian mitoribosome, part 1 of 2 Deposited 2014-09-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 28 PDB declaration: 32-meric
Chain U 1–149（149 aa）
Not recorded ZN ZINC ION × 2 MG MAGNESIUM ION × 169 ELECTRON MICROSCOPY
cryo-EM buffer 20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT；pH 7.4；20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；MIXTURE OF LIQUID ETHANE AND PROPANE
Resolution 3.40 Å
5AJ4 Structure of the 55S mammalian mitoribosome. Deposited 2015-02-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 83 PDB declaration: 89-meric
Chain BU 1–149（149 aa）
Not recorded MG MAGNESIUM ION × 351 ZN ZINC ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer 20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT；pH 7.4；20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；MIXTURE OF LIQUID ETHANE AND PROPANE
Resolution 3.80 Å

2 other PDB entries and 2 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name F1RJD0_PIG
Isoform —
PDB entities 23
Chains and sequence ranges Author chain U； PDBConstruct 1–124； UniProt 1–124

MRPL21

OrganismNot specified

UniProt F1RY70

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 36 RNA 2 PDB declaration: 38-meric（38） Consistent with all polymer counts Chain V； UniProt 1–203 Not recorded MRPL27 × 1 （F1RT79） MRPL28 × 1 （F1RGV8） MRPL47 × 1 MRPL30 × 1 （F1STE5） MRPL32 × 1 （I3LTC4） MRPL33 × 1 MRPL34 × 1 MRPL35 × 1 （F1SVC5） MRPL36 × 1 （I3LDE8） 16S Ribosomal RNA × 1 UNASSIGNED RNA × 1 MRPL2 × 1 （F1RRN2） MRPL3 × 1 （F1RX24） MRPL4 × 1 （F1S3J8） MRPL9 × 1 （I3LR45） MRPL13 × 1 （F1S286） MRPL14 × 1 （F1RQV7） MRPL15 × 1 （F1RSH0） MRPL16 × 1 （F1RI89） MRPL17 × 1 （F1RMQ4） MRPL18 × 1 （F1SB64） MRPL19 × 1 （I3LNJ0） MRPL20 × 1 （F1RJD0） MRPL22 × 1 MRPL23 × 1 MRPL24 × 1 （F1RHJ1） MRPL38 × 1 （F1RW03） MRPL39 × 1 （F1SHP6） MRPL44 × 1 （F1SR64） MRPL45 × 1 MRPL49 × 1 MRPL52 × 1 ICT1 × 1 UNASSIGNED HELICES × 2 THIOREDOXIN FOLD × 1 UNASSIGNED HELICES × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY cryo-EM buffer：20 MM HEPES-KOH PH 7.9, 100 MM KCL, 1 MM DTT, 20 MM MGCL2；pH 7.9；20 MM HEPES-KOH PH 7.9, 100 MM KCL, 1 MM DTT, 20 MM MGCL2 cryo-EM vitrification conditions：Cryogen ETHANE；MANUAL BLOTTING FOLLOWED BY MANUAL PLUNGING INTO LIQUID ETHANE Resolution 4.90 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4CE4 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4V19 Structure of the large subunit of the mammalian mitoribosome, part 1 of 2 Deposited 2014-09-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 28 PDB declaration: 32-meric
Chain V 1–209（209 aa）
Not recorded ZN ZINC ION × 2 MG MAGNESIUM ION × 169 ELECTRON MICROSCOPY
cryo-EM buffer 20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT；pH 7.4；20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；MIXTURE OF LIQUID ETHANE AND PROPANE
Resolution 3.40 Å
5AJ4 Structure of the 55S mammalian mitoribosome. Deposited 2015-02-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 83 PDB declaration: 89-meric
Chain BV 1–209（209 aa）
Not recorded MG MAGNESIUM ION × 351 ZN ZINC ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer 20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT；pH 7.4；20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；MIXTURE OF LIQUID ETHANE AND PROPANE
Resolution 3.80 Å
6GAW Unique features of mammalian mitochondrial translation initiation revealed by cryo-EM. This file contains the complete 55S ribosome. Deposited 2018-04-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 90 PDB declaration: 95-meric
Chain BV 1–209（209 aa）
Not recorded MG MAGNESIUM ION × 326 ZN ZINC ION × 6 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 3 GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 1 NA SODIUM ION × 1 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6GB2 Unique features of mammalian mitochondrial translation initiation revealed by cryo-EM. This file contains the 39S ribosomal subunit. Deposited 2018-04-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 59 PDB declaration: 62-meric
Chain BV 1–209（209 aa）
Not recorded MG MAGNESIUM ION × 216 ZN ZINC ION × 3 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 2 GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 1 NA SODIUM ION × 1 FME N-FORMYLMETHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6YDP 55S mammalian mitochondrial ribosome with mtEFG1 and P site fMet-tRNAMet (POST) Deposited 2020-03-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 89 PDB declaration: 94-meric
Chain BV 1–209（209 aa）
Not recorded MG MAGNESIUM ION × 326 ZN ZINC ION × 6 SPM SPERMINE × 3 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.00 Å
6YDW 55S mammalian mitochondrial ribosome with mtEFG1 and two tRNAMet (TI-POST) Deposited 2020-03-21 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 89 PDB declaration: 95-meric
Chain BV 1–209（209 aa）
Not recorded MG MAGNESIUM ION × 325 ZN ZINC ION × 6 SPM SPERMINE × 3 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.20 Å
7NQH 55S mammalian mitochondrial ribosome with mtRF1a and P-site tRNAMet Deposited 2021-03-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 93-meric
Chain BV 1–209（209 aa）
Not recorded MG MAGNESIUM ION × 323 ZN ZINC ION × 6 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.50 Å
7NQL 55S mammalian mitochondrial ribosome with ICT1 and P site tRNAMet Deposited 2021-03-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 93-meric
Chain BV 1–209（209 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 335 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.40 Å
7NSH 39S mammalian mitochondrial large ribosomal subunit with mtRRF (post) and mtEFG2 Deposited 2021-03-07 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 59 PDB declaration: 61-meric
Chain BV 1–209（209 aa）
Not recorded MG MAGNESIUM ION × 221 ZN ZINC ION × 3 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 2 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.20 Å
7NSI 55S mammalian mitochondrial ribosome with mtRRF (pre) and tRNA(P/E) Deposited 2021-03-07 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 93-meric
Chain BV 1–209（209 aa）
Not recorded MG MAGNESIUM ION × 327 ZN ZINC ION × 6 SPM SPERMINE × 3 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.60 Å
7NSJ 55S mammalian mitochondrial ribosome with tRNA(P/P) and tRNA(E*) Deposited 2021-03-07 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 87 PDB declaration: 93-meric
Chain BV 1–209（209 aa）
Not recorded MG MAGNESIUM ION × 324 ZN ZINC ION × 6 SPM SPERMINE × 3 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.90 Å
8OIN 55S mammalian mitochondrial ribosome with mtRF1 and P-site tRNA Deposited 2023-03-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 94-meric
Chain BZ 1–209（209 aa）
Not recorded MG MAGNESIUM ION × 332 K POTASSIUM ION × 41 PHE PHENYLALANINE × 1 ZN ZINC ION × 3 FES FE2/S2 (INORGANIC) CLUSTER × 3 SPM SPERMINE × 1 SPD SPERMIDINE × 1 FME N-FORMYLMETHIONINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20mM HEPES-KOH pH=7.5, 100mM KCl, 20mM MgCl2, ~1% sucrose (from mtRF1 storage buffer)
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.60 Å
8OIQ 39S mammalian mitochondrial large ribosomal subunit with mtRF1 and P-site tRNA Deposited 2023-03-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 58 PDB declaration: 62-meric
Chain BZ 1–209（209 aa）
Not recorded MG MAGNESIUM ION × 205 K POTASSIUM ION × 30 PHE PHENYLALANINE × 1 ZN ZINC ION × 2 FES FE2/S2 (INORGANIC) CLUSTER × 1 FME N-FORMYLMETHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20mM HEPES-KOH pH=7.5, 100mM KCl, 20mM MgCl2, ~1% sucrose (from mtRF1 storage buffer)
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.50 Å

13 other PDB entries and 13 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name F1RY70_PIG
Isoform —
PDB entities 24
Chains and sequence ranges Author chain V； PDBConstruct 1–203； UniProt 1–203

MRPL24

OrganismNot specified

UniProt F1RHJ1

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 36 RNA 2 PDB declaration: 38-meric（38） Consistent with all polymer counts Chain Y； UniProt 1–216 Not recorded MRPL27 × 1 （F1RT79） MRPL28 × 1 （F1RGV8） MRPL47 × 1 MRPL30 × 1 （F1STE5） MRPL32 × 1 （I3LTC4） MRPL33 × 1 MRPL34 × 1 MRPL35 × 1 （F1SVC5） MRPL36 × 1 （I3LDE8） 16S Ribosomal RNA × 1 UNASSIGNED RNA × 1 MRPL2 × 1 （F1RRN2） MRPL3 × 1 （F1RX24） MRPL4 × 1 （F1S3J8） MRPL9 × 1 （I3LR45） MRPL13 × 1 （F1S286） MRPL14 × 1 （F1RQV7） MRPL15 × 1 （F1RSH0） MRPL16 × 1 （F1RI89） MRPL17 × 1 （F1RMQ4） MRPL18 × 1 （F1SB64） MRPL19 × 1 （I3LNJ0） MRPL20 × 1 （F1RJD0） MRPL21 × 1 （F1RY70） MRPL22 × 1 MRPL23 × 1 MRPL38 × 1 （F1RW03） MRPL39 × 1 （F1SHP6） MRPL44 × 1 （F1SR64） MRPL45 × 1 MRPL49 × 1 MRPL52 × 1 ICT1 × 1 UNASSIGNED HELICES × 2 THIOREDOXIN FOLD × 1 UNASSIGNED HELICES × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY cryo-EM buffer：20 MM HEPES-KOH PH 7.9, 100 MM KCL, 1 MM DTT, 20 MM MGCL2；pH 7.9；20 MM HEPES-KOH PH 7.9, 100 MM KCL, 1 MM DTT, 20 MM MGCL2 cryo-EM vitrification conditions：Cryogen ETHANE；MANUAL BLOTTING FOLLOWED BY MANUAL PLUNGING INTO LIQUID ETHANE Resolution 4.90 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4CE4 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4V19 Structure of the large subunit of the mammalian mitoribosome, part 1 of 2 Deposited 2014-09-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 28 PDB declaration: 32-meric
Chain Y 1–216（216 aa）
Not recorded ZN ZINC ION × 2 MG MAGNESIUM ION × 169 ELECTRON MICROSCOPY
cryo-EM buffer 20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT；pH 7.4；20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；MIXTURE OF LIQUID ETHANE AND PROPANE
Resolution 3.40 Å
5AJ4 Structure of the 55S mammalian mitoribosome. Deposited 2015-02-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 83 PDB declaration: 89-meric
Chain BY 1–216（216 aa）
Not recorded MG MAGNESIUM ION × 351 ZN ZINC ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer 20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT；pH 7.4；20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；MIXTURE OF LIQUID ETHANE AND PROPANE
Resolution 3.80 Å
6GAW Unique features of mammalian mitochondrial translation initiation revealed by cryo-EM. This file contains the complete 55S ribosome. Deposited 2018-04-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 90 PDB declaration: 95-meric
Chain BY 1–216（216 aa）
Not recorded MG MAGNESIUM ION × 326 ZN ZINC ION × 6 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 3 GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 1 NA SODIUM ION × 1 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6GB2 Unique features of mammalian mitochondrial translation initiation revealed by cryo-EM. This file contains the 39S ribosomal subunit. Deposited 2018-04-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 59 PDB declaration: 62-meric
Chain BY 1–216（216 aa）
Not recorded MG MAGNESIUM ION × 216 ZN ZINC ION × 3 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 2 GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 1 NA SODIUM ION × 1 FME N-FORMYLMETHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6YDP 55S mammalian mitochondrial ribosome with mtEFG1 and P site fMet-tRNAMet (POST) Deposited 2020-03-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 89 PDB declaration: 94-meric
Chain BY 1–216（216 aa）
Not recorded MG MAGNESIUM ION × 326 ZN ZINC ION × 6 SPM SPERMINE × 3 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.00 Å
6YDW 55S mammalian mitochondrial ribosome with mtEFG1 and two tRNAMet (TI-POST) Deposited 2020-03-21 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 89 PDB declaration: 95-meric
Chain BY 1–216（216 aa）
Not recorded MG MAGNESIUM ION × 325 ZN ZINC ION × 6 SPM SPERMINE × 3 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.20 Å
7NQH 55S mammalian mitochondrial ribosome with mtRF1a and P-site tRNAMet Deposited 2021-03-01 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 93-meric
Chain BY 1–216（216 aa）
Not recorded MG MAGNESIUM ION × 323 ZN ZINC ION × 6 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.50 Å
7NQL 55S mammalian mitochondrial ribosome with ICT1 and P site tRNAMet Deposited 2021-03-01 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 93-meric
Chain BY 1–216（216 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 335 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.40 Å
7NSH 39S mammalian mitochondrial large ribosomal subunit with mtRRF (post) and mtEFG2 Deposited 2021-03-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 59 PDB declaration: 61-meric
Chain BY 1–216（216 aa）
Not recorded MG MAGNESIUM ION × 221 ZN ZINC ION × 3 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 2 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.20 Å
7NSI 55S mammalian mitochondrial ribosome with mtRRF (pre) and tRNA(P/E) Deposited 2021-03-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 93-meric
Chain BY 1–216（216 aa）
Not recorded MG MAGNESIUM ION × 327 ZN ZINC ION × 6 SPM SPERMINE × 3 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.60 Å
7NSJ 55S mammalian mitochondrial ribosome with tRNA(P/P) and tRNA(E*) Deposited 2021-03-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 87 PDB declaration: 93-meric
Chain BY 1–216（216 aa）
Not recorded MG MAGNESIUM ION × 324 ZN ZINC ION × 6 SPM SPERMINE × 3 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.90 Å
8OIN 55S mammalian mitochondrial ribosome with mtRF1 and P-site tRNA Deposited 2023-03-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 94-meric
Chain BC 1–216（216 aa）
Not recorded MG MAGNESIUM ION × 332 K POTASSIUM ION × 41 PHE PHENYLALANINE × 1 ZN ZINC ION × 3 FES FE2/S2 (INORGANIC) CLUSTER × 3 SPM SPERMINE × 1 SPD SPERMIDINE × 1 FME N-FORMYLMETHIONINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20mM HEPES-KOH pH=7.5, 100mM KCl, 20mM MgCl2, ~1% sucrose (from mtRF1 storage buffer)
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.60 Å
8OIQ 39S mammalian mitochondrial large ribosomal subunit with mtRF1 and P-site tRNA Deposited 2023-03-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 58 PDB declaration: 62-meric
Chain BC 1–216（216 aa）
Not recorded MG MAGNESIUM ION × 205 K POTASSIUM ION × 30 PHE PHENYLALANINE × 1 ZN ZINC ION × 2 FES FE2/S2 (INORGANIC) CLUSTER × 1 FME N-FORMYLMETHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20mM HEPES-KOH pH=7.5, 100mM KCl, 20mM MgCl2, ~1% sucrose (from mtRF1 storage buffer)
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.50 Å

13 other PDB entries and 13 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name F1RHJ1_PIG
Isoform —
PDB entities 27
Chains and sequence ranges Author chain Y； PDBConstruct 1–216； UniProt 1–216

MRPL38

OrganismNot specified

UniProt F1RW03

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 36 RNA 2 PDB declaration: 38-meric（38） Consistent with all polymer counts Chain b； UniProt 1–380 Not recorded MRPL27 × 1 （F1RT79） MRPL28 × 1 （F1RGV8） MRPL47 × 1 MRPL30 × 1 （F1STE5） MRPL32 × 1 （I3LTC4） MRPL33 × 1 MRPL34 × 1 MRPL35 × 1 （F1SVC5） MRPL36 × 1 （I3LDE8） 16S Ribosomal RNA × 1 UNASSIGNED RNA × 1 MRPL2 × 1 （F1RRN2） MRPL3 × 1 （F1RX24） MRPL4 × 1 （F1S3J8） MRPL9 × 1 （I3LR45） MRPL13 × 1 （F1S286） MRPL14 × 1 （F1RQV7） MRPL15 × 1 （F1RSH0） MRPL16 × 1 （F1RI89） MRPL17 × 1 （F1RMQ4） MRPL18 × 1 （F1SB64） MRPL19 × 1 （I3LNJ0） MRPL20 × 1 （F1RJD0） MRPL21 × 1 （F1RY70） MRPL22 × 1 MRPL23 × 1 MRPL24 × 1 （F1RHJ1） MRPL39 × 1 （F1SHP6） MRPL44 × 1 （F1SR64） MRPL45 × 1 MRPL49 × 1 MRPL52 × 1 ICT1 × 1 UNASSIGNED HELICES × 2 THIOREDOXIN FOLD × 1 UNASSIGNED HELICES × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY cryo-EM buffer：20 MM HEPES-KOH PH 7.9, 100 MM KCL, 1 MM DTT, 20 MM MGCL2；pH 7.9；20 MM HEPES-KOH PH 7.9, 100 MM KCL, 1 MM DTT, 20 MM MGCL2 cryo-EM vitrification conditions：Cryogen ETHANE；MANUAL BLOTTING FOLLOWED BY MANUAL PLUNGING INTO LIQUID ETHANE Resolution 4.90 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4CE4 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4V1A Structure of the large subunit of the mammalian mitoribosome, part 2 of 2 Deposited 2014-09-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain b 1–380（380 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer 20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT；pH 7.4；20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；MIXTURE OF LIQUID ETHANE AND PROPANE
Resolution 3.40 Å
5AJ4 Structure of the 55S mammalian mitoribosome. Deposited 2015-02-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 83 PDB declaration: 89-meric
Chain Bb 1–380（380 aa）
Not recorded MG MAGNESIUM ION × 351 ZN ZINC ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer 20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT；pH 7.4；20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；MIXTURE OF LIQUID ETHANE AND PROPANE
Resolution 3.80 Å
6GAW Unique features of mammalian mitochondrial translation initiation revealed by cryo-EM. This file contains the complete 55S ribosome. Deposited 2018-04-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 90 PDB declaration: 95-meric
Chain Bb 1–380（380 aa）
Not recorded MG MAGNESIUM ION × 326 ZN ZINC ION × 6 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 3 GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 1 NA SODIUM ION × 1 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6GB2 Unique features of mammalian mitochondrial translation initiation revealed by cryo-EM. This file contains the 39S ribosomal subunit. Deposited 2018-04-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 59 PDB declaration: 62-meric
Chain Bb 1–380（380 aa）
Not recorded MG MAGNESIUM ION × 216 ZN ZINC ION × 3 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 2 GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 1 NA SODIUM ION × 1 FME N-FORMYLMETHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6YDP 55S mammalian mitochondrial ribosome with mtEFG1 and P site fMet-tRNAMet (POST) Deposited 2020-03-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 89 PDB declaration: 94-meric
Chain Bb 1–380（380 aa）
Not recorded MG MAGNESIUM ION × 326 ZN ZINC ION × 6 SPM SPERMINE × 3 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.00 Å
6YDW 55S mammalian mitochondrial ribosome with mtEFG1 and two tRNAMet (TI-POST) Deposited 2020-03-21 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 89 PDB declaration: 95-meric
Chain Bb 1–380（380 aa）
Not recorded MG MAGNESIUM ION × 325 ZN ZINC ION × 6 SPM SPERMINE × 3 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.20 Å
7NQH 55S mammalian mitochondrial ribosome with mtRF1a and P-site tRNAMet Deposited 2021-03-01 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 93-meric
Chain Bb 1–380（380 aa）
Not recorded MG MAGNESIUM ION × 323 ZN ZINC ION × 6 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.50 Å
7NQL 55S mammalian mitochondrial ribosome with ICT1 and P site tRNAMet Deposited 2021-03-01 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 93-meric
Chain Bb 1–380（380 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 335 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.40 Å
7NSH 39S mammalian mitochondrial large ribosomal subunit with mtRRF (post) and mtEFG2 Deposited 2021-03-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 59 PDB declaration: 61-meric
Chain Bb 1–380（380 aa）
Not recorded MG MAGNESIUM ION × 221 ZN ZINC ION × 3 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 2 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.20 Å
7NSI 55S mammalian mitochondrial ribosome with mtRRF (pre) and tRNA(P/E) Deposited 2021-03-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 93-meric
Chain Bb 1–380（380 aa）
Not recorded MG MAGNESIUM ION × 327 ZN ZINC ION × 6 SPM SPERMINE × 3 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.60 Å
7NSJ 55S mammalian mitochondrial ribosome with tRNA(P/P) and tRNA(E*) Deposited 2021-03-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 87 PDB declaration: 93-meric
Chain Bb 1–380（380 aa）
Not recorded MG MAGNESIUM ION × 324 ZN ZINC ION × 6 SPM SPERMINE × 3 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.90 Å
8OIN 55S mammalian mitochondrial ribosome with mtRF1 and P-site tRNA Deposited 2023-03-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 94-meric
Chain Bn 1–380（380 aa）
Not recorded MG MAGNESIUM ION × 332 K POTASSIUM ION × 41 PHE PHENYLALANINE × 1 ZN ZINC ION × 3 FES FE2/S2 (INORGANIC) CLUSTER × 3 SPM SPERMINE × 1 SPD SPERMIDINE × 1 FME N-FORMYLMETHIONINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20mM HEPES-KOH pH=7.5, 100mM KCl, 20mM MgCl2, ~1% sucrose (from mtRF1 storage buffer)
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.60 Å
8OIQ 39S mammalian mitochondrial large ribosomal subunit with mtRF1 and P-site tRNA Deposited 2023-03-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 58 PDB declaration: 62-meric
Chain Bn 1–380（380 aa）
Not recorded MG MAGNESIUM ION × 205 K POTASSIUM ION × 30 PHE PHENYLALANINE × 1 ZN ZINC ION × 2 FES FE2/S2 (INORGANIC) CLUSTER × 1 FME N-FORMYLMETHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20mM HEPES-KOH pH=7.5, 100mM KCl, 20mM MgCl2, ~1% sucrose (from mtRF1 storage buffer)
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.50 Å

13 other PDB entries and 13 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name F1RW03_PIG
Isoform —
PDB entities 28
Chains and sequence ranges Author chain b； PDBConstruct 1–380； UniProt 1–380

MRPL39

OrganismNot specified

UniProt F1SHP6

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 36 RNA 2 PDB declaration: 38-meric（38） Consistent with all polymer counts Chain c； UniProt 69–337 Not recorded MRPL27 × 1 （F1RT79） MRPL28 × 1 （F1RGV8） MRPL47 × 1 MRPL30 × 1 （F1STE5） MRPL32 × 1 （I3LTC4） MRPL33 × 1 MRPL34 × 1 MRPL35 × 1 （F1SVC5） MRPL36 × 1 （I3LDE8） 16S Ribosomal RNA × 1 UNASSIGNED RNA × 1 MRPL2 × 1 （F1RRN2） MRPL3 × 1 （F1RX24） MRPL4 × 1 （F1S3J8） MRPL9 × 1 （I3LR45） MRPL13 × 1 （F1S286） MRPL14 × 1 （F1RQV7） MRPL15 × 1 （F1RSH0） MRPL16 × 1 （F1RI89） MRPL17 × 1 （F1RMQ4） MRPL18 × 1 （F1SB64） MRPL19 × 1 （I3LNJ0） MRPL20 × 1 （F1RJD0） MRPL21 × 1 （F1RY70） MRPL22 × 1 MRPL23 × 1 MRPL24 × 1 （F1RHJ1） MRPL38 × 1 （F1RW03） MRPL44 × 1 （F1SR64） MRPL45 × 1 MRPL49 × 1 MRPL52 × 1 ICT1 × 1 UNASSIGNED HELICES × 2 THIOREDOXIN FOLD × 1 UNASSIGNED HELICES × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY cryo-EM buffer：20 MM HEPES-KOH PH 7.9, 100 MM KCL, 1 MM DTT, 20 MM MGCL2；pH 7.9；20 MM HEPES-KOH PH 7.9, 100 MM KCL, 1 MM DTT, 20 MM MGCL2 cryo-EM vitrification conditions：Cryogen ETHANE；MANUAL BLOTTING FOLLOWED BY MANUAL PLUNGING INTO LIQUID ETHANE Resolution 4.90 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4CE4 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
6GAW Unique features of mammalian mitochondrial translation initiation revealed by cryo-EM. This file contains the complete 55S ribosome. Deposited 2018-04-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 90 PDB declaration: 95-meric
Chain Bc 1–334（334 aa）
Not recorded MG MAGNESIUM ION × 326 ZN ZINC ION × 6 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 3 GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 1 NA SODIUM ION × 1 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6GB2 Unique features of mammalian mitochondrial translation initiation revealed by cryo-EM. This file contains the 39S ribosomal subunit. Deposited 2018-04-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 59 PDB declaration: 62-meric
Chain Bc 1–334（334 aa）
Not recorded MG MAGNESIUM ION × 216 ZN ZINC ION × 3 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 2 GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 1 NA SODIUM ION × 1 FME N-FORMYLMETHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6YDP 55S mammalian mitochondrial ribosome with mtEFG1 and P site fMet-tRNAMet (POST) Deposited 2020-03-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 89 PDB declaration: 94-meric
Chain Bc 1–334（334 aa）
Not recorded MG MAGNESIUM ION × 326 ZN ZINC ION × 6 SPM SPERMINE × 3 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.00 Å
6YDW 55S mammalian mitochondrial ribosome with mtEFG1 and two tRNAMet (TI-POST) Deposited 2020-03-21 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 89 PDB declaration: 95-meric
Chain Bc 1–334（334 aa）
Not recorded MG MAGNESIUM ION × 325 ZN ZINC ION × 6 SPM SPERMINE × 3 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.20 Å
7NQH 55S mammalian mitochondrial ribosome with mtRF1a and P-site tRNAMet Deposited 2021-03-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 93-meric
Chain Bc 1–334（334 aa）
Not recorded MG MAGNESIUM ION × 323 ZN ZINC ION × 6 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.50 Å
7NQL 55S mammalian mitochondrial ribosome with ICT1 and P site tRNAMet Deposited 2021-03-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 93-meric
Chain Bc 1–334（334 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 335 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.40 Å
7NSH 39S mammalian mitochondrial large ribosomal subunit with mtRRF (post) and mtEFG2 Deposited 2021-03-07 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 59 PDB declaration: 61-meric
Chain Bc 1–334（334 aa）
Not recorded MG MAGNESIUM ION × 221 ZN ZINC ION × 3 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 2 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.20 Å
7NSJ 55S mammalian mitochondrial ribosome with tRNA(P/P) and tRNA(E*) Deposited 2021-03-07 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 87 PDB declaration: 93-meric
Chain Bc 1–334（334 aa）
Not recorded MG MAGNESIUM ION × 324 ZN ZINC ION × 6 SPM SPERMINE × 3 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.90 Å
8OIN 55S mammalian mitochondrial ribosome with mtRF1 and P-site tRNA Deposited 2023-03-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 94-meric
Chain Bo 1–334（334 aa）
Not recorded MG MAGNESIUM ION × 332 K POTASSIUM ION × 41 PHE PHENYLALANINE × 1 ZN ZINC ION × 3 FES FE2/S2 (INORGANIC) CLUSTER × 3 SPM SPERMINE × 1 SPD SPERMIDINE × 1 FME N-FORMYLMETHIONINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20mM HEPES-KOH pH=7.5, 100mM KCl, 20mM MgCl2, ~1% sucrose (from mtRF1 storage buffer)
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.60 Å
8OIQ 39S mammalian mitochondrial large ribosomal subunit with mtRF1 and P-site tRNA Deposited 2023-03-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 58 PDB declaration: 62-meric
Chain Bo 1–334（334 aa）
Not recorded MG MAGNESIUM ION × 205 K POTASSIUM ION × 30 PHE PHENYLALANINE × 1 ZN ZINC ION × 2 FES FE2/S2 (INORGANIC) CLUSTER × 1 FME N-FORMYLMETHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20mM HEPES-KOH pH=7.5, 100mM KCl, 20mM MgCl2, ~1% sucrose (from mtRF1 storage buffer)
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.50 Å

10 other PDB entries and 10 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name F1SHP6_PIG
Isoform —
PDB entities 29
Chains and sequence ranges Author chain c； PDBConstruct 33–301； UniProt 69–337

MRPL44

OrganismNot specified

UniProt F1SR64

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 36 RNA 2 PDB declaration: 38-meric（38） Consistent with all polymer counts Chain h； UniProt 67–332 Not recorded MRPL27 × 1 （F1RT79） MRPL28 × 1 （F1RGV8） MRPL47 × 1 MRPL30 × 1 （F1STE5） MRPL32 × 1 （I3LTC4） MRPL33 × 1 MRPL34 × 1 MRPL35 × 1 （F1SVC5） MRPL36 × 1 （I3LDE8） 16S Ribosomal RNA × 1 UNASSIGNED RNA × 1 MRPL2 × 1 （F1RRN2） MRPL3 × 1 （F1RX24） MRPL4 × 1 （F1S3J8） MRPL9 × 1 （I3LR45） MRPL13 × 1 （F1S286） MRPL14 × 1 （F1RQV7） MRPL15 × 1 （F1RSH0） MRPL16 × 1 （F1RI89） MRPL17 × 1 （F1RMQ4） MRPL18 × 1 （F1SB64） MRPL19 × 1 （I3LNJ0） MRPL20 × 1 （F1RJD0） MRPL21 × 1 （F1RY70） MRPL22 × 1 MRPL23 × 1 MRPL24 × 1 （F1RHJ1） MRPL38 × 1 （F1RW03） MRPL39 × 1 （F1SHP6） MRPL45 × 1 MRPL49 × 1 MRPL52 × 1 ICT1 × 1 UNASSIGNED HELICES × 2 THIOREDOXIN FOLD × 1 UNASSIGNED HELICES × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY cryo-EM buffer：20 MM HEPES-KOH PH 7.9, 100 MM KCL, 1 MM DTT, 20 MM MGCL2；pH 7.9；20 MM HEPES-KOH PH 7.9, 100 MM KCL, 1 MM DTT, 20 MM MGCL2 cryo-EM vitrification conditions：Cryogen ETHANE；MANUAL BLOTTING FOLLOWED BY MANUAL PLUNGING INTO LIQUID ETHANE Resolution 4.90 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4CE4 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4V1A Structure of the large subunit of the mammalian mitoribosome, part 2 of 2 Deposited 2014-09-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain h 1–332（332 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer 20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT；pH 7.4；20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；MIXTURE OF LIQUID ETHANE AND PROPANE
Resolution 3.40 Å
5AJ4 Structure of the 55S mammalian mitoribosome. Deposited 2015-02-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 83 PDB declaration: 89-meric
Chain Bh 1–332（332 aa）
Not recorded MG MAGNESIUM ION × 351 ZN ZINC ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer 20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT；pH 7.4；20 MM HEPES-KOH, 50 MM KCL, 40 MM MGCL2, 1 MM DTT
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；MIXTURE OF LIQUID ETHANE AND PROPANE
Resolution 3.80 Å
6GAW Unique features of mammalian mitochondrial translation initiation revealed by cryo-EM. This file contains the complete 55S ribosome. Deposited 2018-04-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 90 PDB declaration: 95-meric
Chain Bh 1–332（332 aa）
Not recorded MG MAGNESIUM ION × 326 ZN ZINC ION × 6 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 3 GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 1 NA SODIUM ION × 1 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6GB2 Unique features of mammalian mitochondrial translation initiation revealed by cryo-EM. This file contains the 39S ribosomal subunit. Deposited 2018-04-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 59 PDB declaration: 62-meric
Chain Bh 1–332（332 aa）
Not recorded MG MAGNESIUM ION × 216 ZN ZINC ION × 3 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 2 GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 1 NA SODIUM ION × 1 FME N-FORMYLMETHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6YDP 55S mammalian mitochondrial ribosome with mtEFG1 and P site fMet-tRNAMet (POST) Deposited 2020-03-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 89 PDB declaration: 94-meric
Chain Bh 1–332（332 aa）
Not recorded MG MAGNESIUM ION × 326 ZN ZINC ION × 6 SPM SPERMINE × 3 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.00 Å
6YDW 55S mammalian mitochondrial ribosome with mtEFG1 and two tRNAMet (TI-POST) Deposited 2020-03-21 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 89 PDB declaration: 95-meric
Chain Bh 1–332（332 aa）
Not recorded MG MAGNESIUM ION × 325 ZN ZINC ION × 6 SPM SPERMINE × 3 FME N-FORMYLMETHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.20 Å
7NQH 55S mammalian mitochondrial ribosome with mtRF1a and P-site tRNAMet Deposited 2021-03-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 93-meric
Chain Bh 1–332（332 aa）
Not recorded MG MAGNESIUM ION × 323 ZN ZINC ION × 6 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.50 Å
7NQL 55S mammalian mitochondrial ribosome with ICT1 and P site tRNAMet Deposited 2021-03-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 93-meric
Chain Bh 1–332（332 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 335 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.40 Å
7NSH 39S mammalian mitochondrial large ribosomal subunit with mtRRF (post) and mtEFG2 Deposited 2021-03-07 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 59 PDB declaration: 61-meric
Chain Bh 1–332（332 aa）
Not recorded MG MAGNESIUM ION × 221 ZN ZINC ION × 3 5GP GUANOSINE-5'-MONOPHOSPHATE × 2 SPM SPERMINE × 2 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.20 Å
7NSI 55S mammalian mitochondrial ribosome with mtRRF (pre) and tRNA(P/E) Deposited 2021-03-07 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 93-meric
Chain Bh 1–332（332 aa）
Not recorded MG MAGNESIUM ION × 327 ZN ZINC ION × 6 SPM SPERMINE × 3 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.60 Å
7NSJ 55S mammalian mitochondrial ribosome with tRNA(P/P) and tRNA(E*) Deposited 2021-03-07 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 87 PDB declaration: 93-meric
Chain Bh 1–332（332 aa）
Not recorded MG MAGNESIUM ION × 324 ZN ZINC ION × 6 SPM SPERMINE × 3 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.90 Å
8OIN 55S mammalian mitochondrial ribosome with mtRF1 and P-site tRNA Deposited 2023-03-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 88 PDB declaration: 94-meric
Chain Bt 1–332（332 aa）
Not recorded MG MAGNESIUM ION × 332 K POTASSIUM ION × 41 PHE PHENYLALANINE × 1 ZN ZINC ION × 3 FES FE2/S2 (INORGANIC) CLUSTER × 3 SPM SPERMINE × 1 SPD SPERMIDINE × 1 FME N-FORMYLMETHIONINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20mM HEPES-KOH pH=7.5, 100mM KCl, 20mM MgCl2, ~1% sucrose (from mtRF1 storage buffer)
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.60 Å
8OIQ 39S mammalian mitochondrial large ribosomal subunit with mtRF1 and P-site tRNA Deposited 2023-03-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 58 PDB declaration: 62-meric
Chain Bt 1–332（332 aa）
Not recorded MG MAGNESIUM ION × 205 K POTASSIUM ION × 30 PHE PHENYLALANINE × 1 ZN ZINC ION × 2 FES FE2/S2 (INORGANIC) CLUSTER × 1 FME N-FORMYLMETHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20mM HEPES-KOH pH=7.5, 100mM KCl, 20mM MgCl2, ~1% sucrose (from mtRF1 storage buffer)
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.50 Å

13 other PDB entries and 13 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name F1SR64_PIG
Isoform —
PDB entities 30
Chains and sequence ranges Author chain h； PDBConstruct 33–298； UniProt 67–332

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

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P(r) Distance Distribution P(r) Distribution

暂无 P(r) 图
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2. Structure Basics 2. Structure Basics

Entry ID entry_id4ce4
Deposition date deposition_date2013-11-08
Structure title title39S large subunit of the porcine mitochondrial ribosome
Keywords keywords;RIBOSOME, MAMMALIAN MITOCHONDRIAL RIBOSOME, 39S LARGE RIBOSOMAL SUBUNIT, TRANSLATION, RIBOSOMAL PROTEINS, RRNA, POLYPEPTIDE EXIT SITE, MEMBRANE ASSOCIATION ;; RIBOSOME
Experimental Method methodELECTRON MICROSCOPY
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

该条目暂无 SAXS 数据。

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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

该条目暂无 P(r) 分析数据。

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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (38)

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8. Citations (1)

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9. Files and Curves (0)