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4k81

Crystal structure of the Grb14 RA and PH domains in complex with GTP-loaded H-Ras

Method: X-RAY DIFFRACTION
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Growth factor receptor-bound protein 14

Homo sapiens

UniProt Q14449

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 2 GTPase HRas × 1 （P01112） GLYCEROL × 5 GUANOSINE-5'-TRIPHOSPHATE × 1 MAGNESIUM ION × 1 water × 2 Consistent with protein count
2 Protein heterocomplex Heteromer Protein 2 GTPase HRas × 1 （P01112） GLYCEROL × 2 GUANOSINE-5'-TRIPHOSPHATE × 1 MAGNESIUM ION × 1 water × 2 Consistent with protein count
3 Protein heterocomplex Heteromer Protein 2 GTPase HRas × 1 （P01112） GLYCEROL × 2 GUANOSINE-5'-TRIPHOSPHATE × 1 MAGNESIUM ION × 1 water × 2 Consistent with protein count
4 Protein heterocomplex Heteromer Protein 2 GTPase HRas × 1 （P01112） GUANOSINE-5'-TRIPHOSPHATE × 1 MAGNESIUM ION × 1 water × 2 Consistent with protein count

Other States of the Same Protein in the Database

Monomer

None

Homodimer
2aug 2aug

2 assemblies

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
2auh 4k81 4k81 4k81 4k81

5 assemblies

View Construct and Data Evidence
UniProt name GRB14_HUMAN
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 8–258； UniProt 106–356 Author chain C； PDBConstruct 8–258； UniProt 106–356 Author chain E； PDBConstruct 8–258； UniProt 106–356 Author chain G； PDBConstruct 8–258； UniProt 106–356

GTPase HRas

Homo sapiens

UniProt P01112

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 2 Growth factor receptor-bound protein 14 × 1 （Q14449） GLYCEROL × 5 GUANOSINE-5'-TRIPHOSPHATE × 1 MAGNESIUM ION × 1 water × 2 Consistent with protein count
2 Protein heterocomplex Heteromer Protein 2 Growth factor receptor-bound protein 14 × 1 （Q14449） GLYCEROL × 2 GUANOSINE-5'-TRIPHOSPHATE × 1 MAGNESIUM ION × 1 water × 2 Consistent with protein count
3 Protein heterocomplex Heteromer Protein 2 Growth factor receptor-bound protein 14 × 1 （Q14449） GLYCEROL × 2 GUANOSINE-5'-TRIPHOSPHATE × 1 MAGNESIUM ION × 1 water × 2 Consistent with protein count
4 Protein heterocomplex Heteromer Protein 2 Growth factor receptor-bound protein 14 × 1 （Q14449） GUANOSINE-5'-TRIPHOSPHATE × 1 MAGNESIUM ION × 1 water × 2 Consistent with protein count

Other States of the Same Protein in the Database

Monomer
10dc 1aa9 1clu 1crp 1crq 1crr 1ctq 1iaq 1iaq 1iaq 1ioz 1lf0

156 assemblies; showing the first 12

Homodimer
121p 1agp 1gnp 1gnq 1gnr 1jah 1jai 1plj 1plk 1pll 1q21 221p

24 assemblies; showing the first 12

Homotetramer
6q21

1 assemblies

Other homooligomers
2cl0 3i3s 3lbh 3lbi 3lbn 3oiu 3oiv 3oiw 4dlr 4dls 4dlt 4dlu

18 assemblies; showing the first 12

Heteromeric or nucleic-acid complex
1bkd 1k8r 1lfd 1lfd 1nvu 1nvu 1nvv 1nvv 1nvv 1nvv 1nvw 1nvw

126 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name RASH_HUMAN
Isoform —
PDB entities 2
Chains and sequence ranges Author chain B； PDBConstruct 6–171； UniProt 1–166 Author chain D； PDBConstruct 6–171； UniProt 1–166 Author chain F； PDBConstruct 6–171； UniProt 1–166 Author chain H； PDBConstruct 6–171； UniProt 1–166

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id4k81
Deposition date deposition_date2013-04-17
Structure title titleCrystal structure of the Grb14 RA and PH domains in complex with GTP-loaded H-Ras
Keywords keywordsADAPTOR PROTEIN, SIGNALING PROTEIN; SIGNALING PROTEIN
Experimental Method methodX-RAY DIFFRACTION
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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

4k81__assembly_4__model_1

Assembly 4 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

4k81__assembly_4__model_1 | I(q)

10-2 10-1 105 106 107 q (1/Angstrom) I(q)
100 plotted points; both axes use logarithmic scales.

4k81__assembly_4__model_1 | P(r) · Pending

The new assembly/model P(r) has not been calculated yet This placeholder does not display legacy data r (Angstrom) P(r)
P(r) will be calculated and displayed separately for the same assembly/model.
Rg（Guinier）27.46 Å
Rg (electron density)26.95 Å
Total Rg27.59 Å
Atom count3408
Residues415
Excluded volume60424 ų
Maximum q0.500 Å⁻¹
Assembly Model Structure unit Oligomeric description Status CRYSOL Actions
1 1 4k81__assembly_1__model_1 dimeric （2） Success 4.1.3-1-20251215 (887e7ef) View Download
2 1 4k81__assembly_2__model_1 dimeric （2） Success 4.1.3-1-20251215 (887e7ef) View Download
3 1 4k81__assembly_3__model_1 dimeric （2） Success 4.1.3-1-20251215 (887e7ef) View Download
4 1 4k81__assembly_4__model_1 dimeric （2） Success 4.1.3-1-20251215 (887e7ef) View Download
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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (6)

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6. Fold Classification (SCOP + CATH) 20 domains

SCOP 2.08 (8 domains)

Domain ID domain_idd4k81b1
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.37 — P-loop containing nucleoside triphosphate hydrolases
Superfamily Superfamily superfamilyc.37.1 — P-loop containing nucleoside triphosphate hydrolases
Family Family familyc.37.1.8 — G proteins
Domain ID domain_idd4k81b2
Class classl — Artifacts
Fold Fold foldl.1 — Tags
Superfamily Superfamily superfamilyl.1.1 — Tags
Family Family familyl.1.1.1 — Tags
Domain ID domain_idd4k81d1
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.37 — P-loop containing nucleoside triphosphate hydrolases
Superfamily Superfamily superfamilyc.37.1 — P-loop containing nucleoside triphosphate hydrolases
Family Family familyc.37.1.8 — G proteins
Domain ID domain_idd4k81d2
Class classl — Artifacts
Fold Fold foldl.1 — Tags
Superfamily Superfamily superfamilyl.1.1 — Tags
Family Family familyl.1.1.1 — Tags
Domain ID domain_idd4k81f1
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.37 — P-loop containing nucleoside triphosphate hydrolases
Superfamily Superfamily superfamilyc.37.1 — P-loop containing nucleoside triphosphate hydrolases
Family Family familyc.37.1.8 — G proteins
Domain ID domain_idd4k81f2
Class classl — Artifacts
Fold Fold foldl.1 — Tags
Superfamily Superfamily superfamilyl.1.1 — Tags
Family Family familyl.1.1.1 — Tags
Domain ID domain_idd4k81h1
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.37 — P-loop containing nucleoside triphosphate hydrolases
Superfamily Superfamily superfamilyc.37.1 — P-loop containing nucleoside triphosphate hydrolases
Family Family familyc.37.1.8 — G proteins
Domain ID domain_idd4k81h2
Class classl — Artifacts
Fold Fold foldl.1 — Tags
Superfamily Superfamily superfamilyl.1.1 — Tags
Family Family familyl.1.1.1 — Tags

CATH v4.4 (12 domains)

Domain ID domain_id4k81A01
Class class3 — Alpha Beta
Architecture architecture10 — Roll
Topology topology20 — Ubiquitin-like (UB roll)
Homologous superfamily homologous superfamily90 — Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1
Domain ID domain_id4k81A02
Class class2 — Mainly Beta
Architecture architecture30 — Roll
Topology topology29 — PH-domain like
Homologous superfamily homologous superfamily30 — Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)
Domain ID domain_id4k81B00
Class class3 — Alpha Beta
Architecture architecture40 — 3-Layer(aba) Sandwich
Topology topology50 — Rossmann fold
Homologous superfamily homologous superfamily300 — P-loop containing nucleotide triphosphate hydrolases
Domain ID domain_id4k81C01
Class class3 — Alpha Beta
Architecture architecture10 — Roll
Topology topology20 — Ubiquitin-like (UB roll)
Homologous superfamily homologous superfamily90 — Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1
Domain ID domain_id4k81C02
Class class2 — Mainly Beta
Architecture architecture30 — Roll
Topology topology29 — PH-domain like
Homologous superfamily homologous superfamily30 — Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)
Domain ID domain_id4k81D00
Class class3 — Alpha Beta
Architecture architecture40 — 3-Layer(aba) Sandwich
Topology topology50 — Rossmann fold
Homologous superfamily homologous superfamily300 — P-loop containing nucleotide triphosphate hydrolases
Domain ID domain_id4k81E01
Class class3 — Alpha Beta
Architecture architecture10 — Roll
Topology topology20 — Ubiquitin-like (UB roll)
Homologous superfamily homologous superfamily90 — Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1
Domain ID domain_id4k81E02
Class class2 — Mainly Beta
Architecture architecture30 — Roll
Topology topology29 — PH-domain like
Homologous superfamily homologous superfamily30 — Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)
Domain ID domain_id4k81F00
Class class3 — Alpha Beta
Architecture architecture40 — 3-Layer(aba) Sandwich
Topology topology50 — Rossmann fold
Homologous superfamily homologous superfamily300 — P-loop containing nucleotide triphosphate hydrolases
Domain ID domain_id4k81G01
Class class3 — Alpha Beta
Architecture architecture10 — Roll
Topology topology20 — Ubiquitin-like (UB roll)
Homologous superfamily homologous superfamily90 — Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1
Domain ID domain_id4k81G02
Class class2 — Mainly Beta
Architecture architecture30 — Roll
Topology topology29 — PH-domain like
Homologous superfamily homologous superfamily30 — Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)
Domain ID domain_id4k81H00
Class class3 — Alpha Beta
Architecture architecture40 — 3-Layer(aba) Sandwich
Topology topology50 — Rossmann fold
Homologous superfamily homologous superfamily300 — P-loop containing nucleotide triphosphate hydrolases
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7. Citations (1)