GTPase HRas
Homo sapiens
State in the Current Structure
| Assembly | Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Associated Components | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|
| 1 | Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count | Chain A； UniProt 1–166 | Fragment：UNP residues 1-166 Mutation：T35S | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 KBF 3-oxidanyl-~{N}-[[(2~{R})-oxolan-2-yl]methyl]naphthalene-2-carboxamide × 1 MG MAGNESIUM ION × 1 | SOLUTION NMR NMR measurement conditions：pH 6.8；278 K；Ionic strength (raw mmCIF value) 50；Pressure ambient NMR sample composition：0.8 mM [U-99% 13C; U-99% 15N] H-RasT35S, 3.0 mM KBFM123, 20 mM sodium phosphate, 40 mM sodium chloride, 8 mM MAGNESIUM ION, 0.8 mM PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER, 80% D2O/20% d6-DMSO | 80% D2O/20% d6-DMSO | Resolution not provided |
Other States of the Same Protein in the Database
Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.
| Other PDB | Difference from Current Entry 5ZC6 | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 10DC H-Ras GTPase R68A bound to GppNHp Deposited 2026-01-13 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
|
Mutation：R68A | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；291.15 K；0.2 M Calcium Acetate, 20% w/v PEG 3350, 0.1% N-octyl glucopyranoside
|
Resolution 2.08 Å R-free 0.215 |
| 121P STRUKTUR UND GUANOSINTRIPHOSPHAT-HYDROLYSEMECHANISMUS DES C-TERMINAL VERKUERZTEN MENSCHLICHEN KREBSPROTEINS P21-H-RAS Deposited 1991-06-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–166（166 aa）
|
Not recorded | MG MAGNESIUM ION × 2 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 2 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.54 Å |
| 1AA9 HUMAN C-HA-RAS(1-171)(DOT)GDP, NMR, MINIMIZED AVERAGE STRUCTURE Deposited 1997-01-27 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–171（171 aa）
Fragment：RESIDUES 1 - 171
|
Not recorded | MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 |
SOLUTION NMR
NMR measurement conditions
pH 5.5；303 K
|
Resolution not provided |
| 1AGP THREE-DIMENSIONAL STRUCTURES AND PROPERTIES OF A TRANSFORMING AND A NONTRANSFORMING GLY-12 MUTANT OF P21-H-RAS Deposited 1993-03-29 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–166（166 aa）
|
Mutation：G12D | MG MAGNESIUM ION × 2 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.30 Å |
| 1BKD COMPLEX OF HUMAN H-RAS WITH HUMAN SOS-1 Deposited 1998-07-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain R
1–166（166 aa）
Fragment：RESIDUES 1-166
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8；pH 8.0
|
Resolution 2.80 Å R-free 0.281 |
| 1CLU H-RAS COMPLEXED WITH DIAMINOBENZOPHENONE-BETA,GAMMA-IMIDO-GTP Deposited 1999-05-03 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
Fragment：CATALYTIC DOMAIN, RESIDUES 1 - 166
|
Mutation：GLY12PRO | MG MAGNESIUM ION × 1 DBG 3-AMINOBENZOPHENONE-4-YL-AMINOHYDROXYPHOSPHINYLAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；SEE REFERENCE DECRIBING THE STRUCTURE AND CITATIONS THEREIN, pH 7.5, VAPOR DIFFUSION, HANGING DROP
|
Resolution 1.70 Å R-free 0.261 |
| 1CRP THE SOLUTION STRUCTURE AND DYNAMICS OF RAS P21. GDP DETERMINED BY HETERONUCLEAR THREE AND FOUR DIMENSIONAL NMR SPECTROSCOPY Deposited 1993-11-24 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
|
Not recorded | MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | SOLUTION NMR mmCIF provides none of the parsed conditions | Resolution not provided |
| 1CRQ THE SOLUTION STRUCTURE AND DYNAMICS OF RAS P21. GDP DETERMINED BY HETERONUCLEAR THREE AND FOUR DIMENSIONAL NMR SPECTROSCOPY Deposited 1993-11-24 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
|
Not recorded | MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | SOLUTION NMR mmCIF provides none of the parsed conditions | Resolution not provided |
| 1CRR THE SOLUTION STRUCTURE AND DYNAMICS OF RAS P21. GDP DETERMINED BY HETERONUCLEAR THREE AND FOUR DIMENSIONAL NMR SPECTROSCOPY Deposited 1993-11-24 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
|
Not recorded | MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | SOLUTION NMR mmCIF provides none of the parsed conditions | Resolution not provided |
| 1CTQ STRUCTURE OF P21RAS IN COMPLEX WITH GPPNHP AT 100 K Deposited 1999-08-20 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
Fragment：RESIDUES 1-166
|
Not recorded | MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
MICROBATCH；pH 7.6；291 K；PROTEIN WAS CRYSTALLIZED FROM 28 % PEG 400, 10MM MGCL 264MM TRIS/HCL, PH 7.6, pH 7.60, micro-batch, temperature 18K
|
Resolution 1.26 Å R-free 0.224 |
| 1GNP X-RAY CRYSTAL STRUCTURE ANALYSIS OF THE CATALYTIC DOMAIN OF THE ONCOGENE PRODUCT P21H-RAS COMPLEXED WITH CAGED GTP AND MANT DGPPNHP Deposited 1995-05-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–166（166 aa）
|
Not recorded | MG MAGNESIUM ION × 2 AGN PHOSPHOAMINOPHOSPHONIC ACID 3'-O-(N-METHYLANTHRANILOYL-2'-DEOXYGUANYLATE ESTER × 2 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.70 Å |
| 1GNQ X-RAY CRYSTAL STRUCTURE ANALYSIS OF THE CATALYTIC DOMAIN OF THE ONCOGENE PRODUCT P21H-RAS COMPLEXED WITH CAGED GTP AND MANT DGPPNHP Deposited 1995-05-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–166（166 aa）
|
Not recorded | MG MAGNESIUM ION × 2 CAG GUANOSINE 5'-TRIPHOSPHATE P3-[1-(2-NITROPHENYL)ETHYL ESTER] × 2 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.50 Å R-free 0.269 |
| 1GNR X-RAY CRYSTAL STRUCTURE ANALYSIS OF THE CATALYTIC DOMAIN OF THE ONCOGENE PRODUCT P21H-RAS COMPLEXED WITH CAGED GTP AND MANT DGPPNHP Deposited 1995-05-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–166（166 aa）
|
Not recorded | MG MAGNESIUM ION × 2 CAG GUANOSINE 5'-TRIPHOSPHATE P3-[1-(2-NITROPHENYL)ETHYL ESTER] × 2 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.85 Å R-free 0.257 |
| 1IAQ C-H-RAS P21 PROTEIN MUTANT WITH THR 35 REPLACED BY SER (T35S) COMPLEXED WITH GUANOSINE-5'-[B,G-IMIDO] TRIPHOSPHATE Deposited 2001-03-23 | Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
Fragment：CATALYTIC DOMAIN (RESIDUES 1-166)
|
Mutation：T35S | MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；PEG1500, calcium chloride, Tris, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K
|
Resolution 2.90 Å R-free 0.282 |
| 1IAQ C-H-RAS P21 PROTEIN MUTANT WITH THR 35 REPLACED BY SER (T35S) COMPLEXED WITH GUANOSINE-5'-[B,G-IMIDO] TRIPHOSPHATE Deposited 2001-03-23 | Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
1–166（166 aa）
Fragment：CATALYTIC DOMAIN (RESIDUES 1-166)
|
Mutation：T35S | MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；PEG1500, calcium chloride, Tris, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K
|
Resolution 2.90 Å R-free 0.282 |
| 1IAQ C-H-RAS P21 PROTEIN MUTANT WITH THR 35 REPLACED BY SER (T35S) COMPLEXED WITH GUANOSINE-5'-[B,G-IMIDO] TRIPHOSPHATE Deposited 2001-03-23 | Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
1–166（166 aa）
Fragment：CATALYTIC DOMAIN (RESIDUES 1-166)
|
Mutation：T35S | MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；PEG1500, calcium chloride, Tris, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K
|
Resolution 2.90 Å R-free 0.282 |
| 1IOZ Crystal Structure of the C-HA-RAS Protein Prepared by the Cell-Free Synthesis Deposited 2001-04-18 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–171（171 aa）
Fragment：RESIDUES 1-171
|
Not recorded | GDP GUANOSINE-5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；calcium acetate, sodium cacodylate, PEG8000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.00 Å R-free 0.283 |
| 1JAH H-RAS P21 PROTEIN MUTANT G12P, COMPLEXED WITH GUANOSINE-5'-[BETA,GAMMA-METHYLENE] TRIPHOSPHATE AND MAGNESIUM Deposited 1996-12-15 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–166（166 aa）
Fragment：CATALYTIC DOMAIN, RESIDUES 1 - 166
|
Mutation：G12P | MG MAGNESIUM ION × 2 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7.5；pH 7.5
|
Resolution 1.80 Å R-free 0.290 |
| 1JAI H-RAS P21 PROTEIN MUTANT G12P, COMPLEXED WITH GUANOSINE-5'-[BETA,GAMMA-METHYLENE] TRIPHOSPHATE AND MANGANESE Deposited 1996-12-15 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–166（166 aa）
Fragment：CATALYTIC DOMAIN, RESIDUES 1 - 166
|
Mutation：G12P | MN MANGANESE (II) ION × 2 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7.5；pH 7.5
|
Resolution 1.80 Å R-free 0.290 |
| 1K8R Crystal structure of Ras-Bry2RBD complex Deposited 2001-10-25 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–166（166 aa）
Fragment：GTP-binding/catalytic domain, residues 1-166
|
Not recorded | MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；290 K；PEG 3350, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 290K
|
Resolution 3.00 Å R-free 0.305 |
| 1LF0 Crystal Structure of RasA59G in the GTP-bound form Deposited 2002-04-10 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
Fragment：Residues 1-166
|
Mutation：A59G | MG MAGNESIUM ION × 1 CA CALCIUM ION × 2 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；295 K；PEG1500, calcium chloride, magnesium chloride, Tris, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 1.70 Å R-free 0.208 |
| 1LF5 Crystal Structure of RasA59G in the GDP-bound Form Deposited 2002-04-10 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
Fragment：Residues 1-166
|
Mutation：A59G | MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；295 K；PEG8000, calcium acetate, Tris, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 1.70 Å R-free 0.245 |
| 1LFD CRYSTAL STRUCTURE OF THE ACTIVE RAS PROTEIN COMPLEXED WITH THE RAS-INTERACTING DOMAIN OF RALGDS Deposited 1998-04-29 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
1–167（167 aa）
Fragment：RESIDUES 1-171
|
Mutation：E31K | MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 6.5；pH 6.5
|
Resolution 2.10 Å R-free 0.282 |
| 1LFD CRYSTAL STRUCTURE OF THE ACTIVE RAS PROTEIN COMPLEXED WITH THE RAS-INTERACTING DOMAIN OF RALGDS Deposited 1998-04-29 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain D
1–167（167 aa）
Fragment：RESIDUES 1-171
|
Mutation：E31K | MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 6.5；pH 6.5
|
Resolution 2.10 Å R-free 0.282 |
| 1NVU Structural evidence for feedback activation by RasGTP of the Ras-specific nucleotide exchange factor SOS Deposited 2003-02-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain Q
1–166（166 aa）
Fragment：RESIDUES 1-166
Chain R
1–166（166 aa）
Fragment：RESIDUES 1-166
|
Mutation：A59G Mutation：A59G | MG MAGNESIUM ION × 1 PO4 PHOSPHATE ION × 5 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；1.2-1.5 M phosphate, 100 mM Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.20 Å R-free 0.211 |
| 1NVU Structural evidence for feedback activation by RasGTP of the Ras-specific nucleotide exchange factor SOS Deposited 2003-02-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric |
Chain Q
1–166（166 aa）
Fragment：RESIDUES 1-166
Chain R
1–166（166 aa）
Fragment：RESIDUES 1-166
|
Mutation：A59G Mutation：A59G | MG MAGNESIUM ION × 4 PO4 PHOSPHATE ION × 20 GTP GUANOSINE-5'-TRIPHOSPHATE × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；1.2-1.5 M phosphate, 100 mM Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.20 Å R-free 0.211 |
| 1NVV Structural evidence for feedback activation by RasGTP of the Ras-specific nucleotide exchange factor SOS Deposited 2003-02-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain Q
1–166（166 aa）
Fragment：RESIDUES 1-166
Chain R
1–166（166 aa）
Fragment：RESIDUES 1-166
|
Mutation：Y64A | MG MAGNESIUM ION × 1 PO4 PHOSPHATE ION × 5 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；1.2-1.5 M phosphate, 100 mM Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.18 Å R-free 0.241 |
| 1NVV Structural evidence for feedback activation by RasGTP of the Ras-specific nucleotide exchange factor SOS Deposited 2003-02-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric |
Chain Q
1–166（166 aa）
Fragment：RESIDUES 1-166
Chain R
1–166（166 aa）
Fragment：RESIDUES 1-166
|
Mutation：Y64A | MG MAGNESIUM ION × 8 PO4 PHOSPHATE ION × 40 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 8 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；1.2-1.5 M phosphate, 100 mM Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.18 Å R-free 0.241 |
| 1NVV Structural evidence for feedback activation by RasGTP of the Ras-specific nucleotide exchange factor SOS Deposited 2003-02-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 3 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric |
Chain Q
1–166（166 aa）
Fragment：RESIDUES 1-166
Chain R
1–166（166 aa）
Fragment：RESIDUES 1-166
|
Mutation：Y64A | MG MAGNESIUM ION × 4 PO4 PHOSPHATE ION × 20 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；1.2-1.5 M phosphate, 100 mM Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.18 Å R-free 0.241 |
| 1NVV Structural evidence for feedback activation by RasGTP of the Ras-specific nucleotide exchange factor SOS Deposited 2003-02-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 4 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric |
Chain Q
1–166（166 aa）
Fragment：RESIDUES 1-166
Chain R
1–166（166 aa）
Fragment：RESIDUES 1-166
|
Mutation：Y64A | MG MAGNESIUM ION × 2 PO4 PHOSPHATE ION × 10 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；1.2-1.5 M phosphate, 100 mM Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.18 Å R-free 0.241 |
| 1NVW Structural evidence for feedback activation by RasGTP of the Ras-specific nucleotide exchange factor SOS Deposited 2003-02-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain Q
1–166（166 aa）
Fragment：RESIDUES 1-166
Chain R
1–166（166 aa）
Fragment：RESIDUES 1-166
|
Not recorded | MG MAGNESIUM ION × 1 PO4 PHOSPHATE ION × 7 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；1.2-1.5 M phosphate, 100 mM Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.70 Å R-free 0.246 |
| 1NVW Structural evidence for feedback activation by RasGTP of the Ras-specific nucleotide exchange factor SOS Deposited 2003-02-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric |
Chain Q
1–166（166 aa）
Fragment：RESIDUES 1-166
Chain R
1–166（166 aa）
Fragment：RESIDUES 1-166
|
Not recorded | MG MAGNESIUM ION × 8 PO4 PHOSPHATE ION × 56 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 8 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；1.2-1.5 M phosphate, 100 mM Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.70 Å R-free 0.246 |
| 1NVW Structural evidence for feedback activation by RasGTP of the Ras-specific nucleotide exchange factor SOS Deposited 2003-02-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 3 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric |
Chain Q
1–166（166 aa）
Fragment：RESIDUES 1-166
Chain R
1–166（166 aa）
Fragment：RESIDUES 1-166
|
Not recorded | MG MAGNESIUM ION × 4 PO4 PHOSPHATE ION × 28 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；1.2-1.5 M phosphate, 100 mM Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.70 Å R-free 0.246 |
| 1NVX Structural evidence for feedback activation by RasGTP of the Ras-specific nucleotide exchange factor SOS Deposited 2003-02-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain Q
1–166（166 aa）
Fragment：RESIDUES 1-166
Chain R
1–166（166 aa）
Fragment：RESIDUES 1-166
|
Mutation：A59G Mutation：A59G | MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PO4 PHOSPHATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；2-8% PEG 4000, 100 mM MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 3.20 Å R-free 0.261 |
| 1P2S H-Ras 166 in 50% 2,2,2 triflouroethanol Deposited 2003-04-16 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
|
Not recorded | MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ETF TRIFLUOROETHANOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.6；298 K；PEG, MAGNESIUM CHLORIDE, DITHIOTHREITOL, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.45 Å R-free 0.230 |
| 1P2T H-Ras 166 in Aqueous mother liqour, RT Deposited 2003-04-16 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
|
Not recorded | MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.6；298 K；PEG, MAGNESIUM CHLORIDE, DITHIOTHREITOL, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.00 Å R-free 0.246 |
| 1P2U H-Ras in 50% isopropanol Deposited 2003-04-16 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
|
Not recorded | MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 IPA ISOPROPYL ALCOHOL × 3 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.00 Å R-free 0.248 |
| 1P2V H-RAS 166 in 60 % 1,6 hexanediol Deposited 2003-04-16 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
|
Not recorded | MG MAGNESIUM ION × 1 HEZ HEXANE-1,6-DIOL × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.30 Å R-free 0.252 |
| 1PLJ CRYSTALLOGRAPHIC STUDIES ON P21H-RAS USING SYNCHROTRON LAUE METHOD: IMPROVEMENT OF CRYSTAL QUALITY AND MONITORING OF THE GTPASE REACTION AT DIFFERENT TIME POINTS Deposited 1994-03-13 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–166（166 aa）
|
Not recorded | MG MAGNESIUM ION × 2 CAG GUANOSINE 5'-TRIPHOSPHATE P3-[1-(2-NITROPHENYL)ETHYL ESTER] × 2 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.80 Å |
| 1PLK CRYSTALLOGRAPHIC STUDIES ON P21H-RAS USING SYNCHROTRON LAUE METHOD: IMPROVEMENT OF CRYSTAL QUALITY AND MONITORING OF THE GTPASE REACTION AT DIFFERENT TIME POINTS Deposited 1994-03-13 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–166（166 aa）
|
Not recorded | MG MAGNESIUM ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.80 Å |
| 1PLL CRYSTALLOGRAPHIC STUDIES ON P21H-RAS USING SYNCHROTRON LAUE METHOD: IMPROVEMENT OF CRYSTAL QUALITY AND MONITORING OF THE GTPASE REACTION AT DIFFERENT TIME POINTS Deposited 1994-03-13 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–166（166 aa）
|
Not recorded | GDP GUANOSINE-5'-DIPHOSPHATE × 2 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.80 Å |
| 1Q21 CRYSTAL STRUCTURES AT 2.2 ANGSTROMS RESOLUTION OF THE CATALYTIC DOMAINS OF NORMAL RAS PROTEIN AND AN ONCOGENIC MUTANT COMPLEXED WITH GSP Deposited 1991-09-25 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–171（171 aa）
|
Not recorded | MG MAGNESIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.20 Å |
| 1QRA STRUCTURE OF P21RAS IN COMPLEX WITH GTP AT 100 K Deposited 1999-06-12 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
Fragment：RESIDUES 1 - 166
|
Not recorded | MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
MICRODIALYSIS；pH 7.6；293 K；PROTEIN WAS CRYSTALLIZED FROM 26 % PEG 400, 10MM MGCL 264MM TRIS/HCL, PH 7.6, MICRODIALYSIS, temperature 293K
|
Resolution 1.60 Å R-free 0.228 |
| 1RVD H-RAS COMPLEXED WITH DIAMINOBENZOPHENONE-BETA,GAMMA-IMIDO-GTP Deposited 1999-05-03 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
Fragment：CATALYTIC DOMAIN, RESIDUES 1 - 166
|
Mutation：GLY12VAL | MG MAGNESIUM ION × 1 DBG 3-AMINOBENZOPHENONE-4-YL-AMINOHYDROXYPHOSPHINYLAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；SEE REFERENCE DECRIBING THE STRUCTURE AND CITATIONS THEREIN, pH 7.5, VAPOR DIFFUSION, HANGING DROP
|
Resolution 1.90 Å R-free 0.274 |
| 1WQ1 RAS-RASGAP COMPLEX Deposited 1997-07-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain R
1–166（166 aa）
Fragment：CATALYTIC DOMAIN, RESIDUES 1 - 166
|
Not recorded | MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 AF3 ALUMINUM FLUORIDE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8；SEE REF. DESCRIBING THE STRUCTURE, pH 8.
|
Resolution 2.50 Å R-free 0.319 |
| 1XCM Crystal structure of the GppNHp-bound H-Ras G60A mutant Deposited 2004-09-02 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–167（167 aa）
|
Mutation：G60A Non-standard monomer：Yes (specific site not provided by mmCIF) | MG MAGNESIUM ION × 2 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；PEG 2000 MME, magnesium sulfate, HEPES, hydrogen peroxide, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.84 Å R-free 0.244 |
| 1XD2 Crystal Structure of a ternary Ras:SOS:Ras*GDP complex Deposited 2004-09-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
1–166（166 aa）
Fragment：residues 1-166
Chain B
1–166（166 aa）
Fragment：residues 1-166
|
Mutation：Y64A | MG MAGNESIUM ION × 1 PO4 PHOSPHATE ION × 4 GDP GUANOSINE-5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；Hepes, Na/K phosphate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.70 Å R-free 0.245 |
| 1XJ0 Crystal Structure of the GDP-bound form of the RasG60A mutant Deposited 2004-09-22 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
Fragment：Residues 1-166
|
Mutation：G60A Non-standard monomer：Yes (specific site not provided by mmCIF) | MG MAGNESIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；PEG 4000, magnesium sulfate, Tris-HCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.70 Å R-free 0.239 |
| 1ZVQ Structure of the Q61G mutant of Ras in the GDP-bound form Deposited 2005-06-02 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
|
Mutation：Q61G | MG MAGNESIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；18% PEG4000, 50 mM Ca Acetate, 10 mM MgCl2, 100 mM Tris-HCl, 250 mM NaCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.00 Å R-free 0.229 |
| 1ZW6 Crystal Structure of the GTP-bound form of RasQ61G Deposited 2005-06-03 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
|
Mutation：Q61G Non-standard monomer：Yes (specific site not provided by mmCIF) | MG MAGNESIUM ION × 3 CA CALCIUM ION × 2 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；22% PEG4000, 200mM Ca Acetate, 100 mM Tris-HCl, 10 mM MgCl2, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.50 Å R-free 0.174 |
| 221P THREE-DIMENSIONAL STRUCTURES OF H-RAS P21 MUTANTS: MOLECULAR BASIS FOR THEIR INABILITY TO FUNCTION AS SIGNAL SWITCH MOLECULES Deposited 1991-06-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–166（166 aa）
|
Mutation：D38E | MG MAGNESIUM ION × 2 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.30 Å |
| 2C5L Structure of PLC epsilon Ras association domain with hRas Deposited 2005-10-27 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–166（166 aa）
|
Mutation：YES | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GOL GLYCEROL × 3 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.90 Å R-free 0.228 |
| 2C5L Structure of PLC epsilon Ras association domain with hRas Deposited 2005-10-27 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
1–166（166 aa）
|
Mutation：YES | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GOL GLYCEROL × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.90 Å R-free 0.228 |
| 2CE2 CRYSTAL STRUCTURE ANALYSIS OF A FLUORESCENT FORM OF H-RAS P21 IN COMPLEX WITH GDP Deposited 2006-02-02 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain X
1–166（166 aa）
Fragment：RESIDUES 1-166
|
Mutation：YES | MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 XY2 N,N'-DIMETHYL-N-(ACETYL)-N'-(7-NITROBENZ-2-OXA-1,3-DIAZOL-4-YL)ETHYLENEDIAMINE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.6；291 K；PROTEIN SOLUTION: 17.22 MG/ML PROTEIN, 64 MM TRIS PH 7.6, 20 MM MAGNESIUM CHLORIDE, 10 MM DTT, 0,1 MM SODIUM AZIDE; RESERVOIR SOLUTION: 64 MM TRIS PH 7.6, 20 MM MAGNESIUM CHLORIDE, 10 MM DTT, 0,1 MM SODIUM AZIDE, 35% PEG 400 MIXTURE OF EQUAL VOLUMES OF PROTEIN AND RESERVOIR SOLUTION, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 291.15K
|
Resolution 1.00 Å R-free 0.163 |
| 2CL0 CRYSTAL STRUCTURE ANALYSIS OF A FLUORESCENT FORM OF H-RAS P21 IN COMPLEX WITH GppNHp Deposited 2006-04-25 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain X
1–166（166 aa）
Fragment：RESIDUES 1-166
|
Mutation：YES | MG MAGNESIUM ION × 15 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 3 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 3 XY2 N,N'-DIMETHYL-N-(ACETYL)-N'-(7-NITROBENZ-2-OXA-1,3-DIAZOL-4-YL)ETHYLENEDIAMINE × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7.6；RESERVOIR SOLUTION: 64 MM TRIS PH 7.6, 20 MM MAGNESIUM CHLORIDE, 10 MM DTT, 0.1 MM SODIUM AZIDE, 26% PEG 400; PROTEIN SOLUTION: 64 MM TRIS PH 7.6, 20 MM MAGNESIUM CHLORIDE, 10 MM DTT, 0.1 MM SODIUM AZIDE
|
Resolution 1.80 Å R-free 0.185 |
| 2CL6 CRYSTAL STRUCTURE ANALYSIS OF A FLUORESCENT FORM OF H-RAS P21 IN COMPLEX WITH S-caged GTP Deposited 2006-04-26 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain X
1–166（166 aa）
Fragment：RESIDUES 1-166
|
Mutation：YES | CAG GUANOSINE 5'-TRIPHOSPHATE P3-[1-(2-NITROPHENYL)ETHYL ESTER] × 1 MG MAGNESIUM ION × 1 XY2 N,N'-DIMETHYL-N-(ACETYL)-N'-(7-NITROBENZ-2-OXA-1,3-DIAZOL-4-YL)ETHYLENEDIAMINE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.6；291.15 K；PROTEIN SOLUTION: 64 MM TRIS PH 7.6, 20 MM MAGNESIUM CHLORIDE, 10 MM DTT, 0,1 MM SODIUM AZIDE; RESERVOIR SOLUTION: 100 MM HEPES PH 7.4, 200 MM MAGNESIUM ACETATE, 17% PEG 8000 (FRESHLY PREPARED) MIXTURE OF EQUAL VOLUMES OF PROTEIN AND RESERVOIR SOLUTION, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 291.15K
|
Resolution 1.24 Å R-free 0.186 |
| 2CL7 CRYSTAL STRUCTURE ANALYSIS OF A FLUORESCENT FORM OF H-RAS P21 IN COMPLEX WITH GTP Deposited 2006-04-26 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain X
1–166（166 aa）
Fragment：RESIDUES 1-166
|
Mutation：YES | MG MAGNESIUM ION × 2 XY2 N,N'-DIMETHYL-N-(ACETYL)-N'-(7-NITROBENZ-2-OXA-1,3-DIAZOL-4-YL)ETHYLENEDIAMINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.2；291.15 K；PROTEIN SOLUTION: 13.35 MG/ML PROTEIN, 64 MM TRIS PH 7.6, 20 MM MAGNESIUM CHLORIDE, 10 MM DTT, 0,1 MM SODIUM AZIDE; RESERVOIR SOLUTION: 100 MM HEPES PH 7.2, 200 MM MAGNESIUM ACETATE, 16% PEG 8000 (FRESHLY PREPARED) MIXTURE OF EQUAL VOLUMES OF PROTEIN AND RESERVOIR SOLUTION, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 291.15K
|
Resolution 1.25 Å R-free 0.170 |
| 2CLC CRYSTAL STRUCTURE ANALYSIS OF A FLUORESCENT FORM OF H-RAS P21 IN COMPLEX WITH GTP (2) Deposited 2006-04-27 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain X
1–166（166 aa）
Fragment：RESIDUES 1-166
|
Mutation：YES | MG MAGNESIUM ION × 2 XY2 N,N'-DIMETHYL-N-(ACETYL)-N'-(7-NITROBENZ-2-OXA-1,3-DIAZOL-4-YL)ETHYLENEDIAMINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.2；291.15 K；PROTEIN SOLUTION: 13.35 MG/ML PROTEIN, 64 MM TRIS PH 7.6, 20 MM MAGNESIUM CHLORIDE, 10 MM DTT, 0,1 MM SODIUM AZIDE; RESERVOIR SOLUTION: 100 MM HEPES PH 7.2, 200 MM MAGNESIUM ACETATE, 16% PEG 8000 FRESHLY PREPARED) MIXTURE OF EQUAL VOLUMES OF PROTEIN AND RESERVOIR SOLUTION, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 291.15K
|
Resolution 1.30 Å R-free 0.180 |
| 2CLD CRYSTAL STRUCTURE ANALYSIS OF A FLUORESCENT FORM OF H-RAS P21 IN COMPLEX WITH GDP (2) Deposited 2006-04-27 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain X
1–166（166 aa）
Fragment：RESIDUES 1-166
|
Mutation：YES | MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.2；291.15 K；PROTEIN SOLUTION: 13.35 MG/ML PROTEIN, 64 MM TRIS PH 7.6, 20 MM MAGNESIUM CHLORIDE, 10 MM DTT, 0,1 MM SODIUM AZIDE; RESERVOIR SOLUTION: 100 MM HEPES PH 7.2, 200 MM MAGNESIUM ACETATE, 16% PEG 8000 (FRESHLY PREPARED) MIXTURE OF EQUAL VOLUMES OF PROTEIN AND RESERVOIR SOLUTION, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 291.15K
|
Resolution 1.22 Å R-free 0.176 |
| 2EVW Crystal structure analysis of a fluorescent form of H-Ras p21 in complex with R-caged GTP Deposited 2005-11-01 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain X
1–166（166 aa）
Fragment：truncated form residues 1-166
|
Mutation：Y32C,C118S | MG MAGNESIUM ION × 1 CAG GUANOSINE 5'-TRIPHOSPHATE P3-[1-(2-NITROPHENYL)ETHYL ESTER] × 1 XY2 N,N'-DIMETHYL-N-(ACETYL)-N'-(7-NITROBENZ-2-OXA-1,3-DIAZOL-4-YL)ETHYLENEDIAMINE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.2；291.15 K；protein solution: 64 mM TRIS pH 7.6, 20 mM magnesium chloride, 10 mM DTT, 0,1 mM sodium azide;
reservoir solution: 100 mM HEPES pH 7.2, 200 mM magnesium acetate, 16% PEG 8000 (freshly prepared)
mixture of equal volumes of protein and reservoir solution, VAPOR DIFFUSION, HANGING DROP, temperature 291.15K
|
Resolution 1.05 Å R-free 0.181 |
| 2LCF Solution structure of GppNHp-bound H-RasT35S mutant protein Deposited 2011-04-28 | Different construct Different ligand/ion Different experimental conditions | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
Fragment：residues 1-166
|
Mutation：T35S | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 |
SOLUTION NMR
NMR measurement conditions
pH 6.8；298 K；Ionic strength (raw mmCIF value) 0.24；Pressure ambient
NMR sample composition
1-2mM [U-98% 13C; U-98% 15N] HRas-1, 1-2mM PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER-2, 10mM MAGNESIUM ION-3, 150mM sodium chloride-4, 25mM sodium phosphate-5, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
1-2mM [U-98% 15N] HRas-6, 1-2mM PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER-7, 10mM MAGNESIUM ION-8, 150mM sodium chloride-9, 25mM sodium phosphate-10, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 2LWI Solution structure of H-RasT35S mutant protein in complex with Kobe2601 Deposited 2012-08-01 | Different construct Different ligand/ion Different experimental conditions | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
Fragment：UNP RESIDUES 1-166
|
Mutation：T35S | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 KOB 2-(2,4-dinitrophenyl)-N-(4-fluorophenyl)hydrazinecarbothioamide × 1 |
SOLUTION NMR
NMR measurement conditions
pH 6.8；278 K；Ionic strength (raw mmCIF value) 0.22；Pressure ambient
NMR sample composition
1 mM [U-98% 13C; U-98% 15N] entity_1-1, 1 mM PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER-2, 8 mM MAGNESIUM ION-3, 120 mM sodium chloride-4, 20 mM sodium phosphate-5, 3.8 mM KOB-6, 80% D2O/20% DMSO_d6 | 80% D2O/20% DMSO_d6
NMR sample composition
1 mM entity_1-7, 1 mM PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER-8, 8 mM MAGNESIUM ION-9, 120 mM sodium chloride-10, 20 mM sodium phosphate-11, 3.8 mM KOB-12, 80% D2O/20% DMSO_d6 | 80% D2O/20% DMSO_d6
|
Resolution not provided |
| 2N42 EC-NMR Structure of Human H-RasT35S mutant protein Determined by Combining Evolutionary Couplings (EC) and Sparse NMR Data Deposited 2015-06-16 | Different ligand/ion Different experimental conditions | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
Fragment：UNP residues 1-166
|
Mutation：T35S | No recorded non-water small molecule | SOLUTION NMR mmCIF provides none of the parsed conditions | Resolution not provided |
| 2N46 EC-NMR Structure of Human H-RasT35S mutant protein Determined by Combining Evolutionary Couplings (EC) and Sparse NMR Data Deposited 2015-06-17 | Different construct Different ligand/ion Different experimental conditions | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
|
Mutation：T35S | No recorded non-water small molecule | SOLUTION NMR mmCIF provides none of the parsed conditions | Resolution not provided |
| 2Q21 CRYSTAL STRUCTURES AT 2.2 ANGSTROMS RESOLUTION OF THE CATALYTIC DOMAINS OF NORMAL RAS PROTEIN AND AN ONCOGENIC MUTANT COMPLEXED WITH GSP Deposited 1991-09-25 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–171（171 aa）
|
Mutation：G12V | MG MAGNESIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.20 Å |
| 2QUZ Crystal Structure of the activating H-RasK117R mutant in Costello Syndrome, bound to Mg-GDP Deposited 2007-08-07 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
Fragment：G-domain, residues 1-166
|
Mutation：K117R | MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；37% PEG3350, 50mM TrisHCl pH8, pH 7.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 1.49 Å R-free 0.222 |
| 2RGA Crystal structure of H-RasQ61I-GppNHp Deposited 2007-10-03 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
Fragment：Residues 1-166
|
Mutation：Q61I | CA CALCIUM ION × 1 MG MAGNESIUM ION × 2 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；291 K；20% PEG 3350, 10 % PEG 6000, 0.2M Calcium chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K
|
Resolution 1.90 Å R-free 0.212 |
| 2RGB Crystal structure of H-RasQ61K-GppNHp Deposited 2007-10-03 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
Fragment：Residues 1-166
|
Mutation：Q61K | CA CALCIUM ION × 1 MG MAGNESIUM ION × 2 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；291 K；20% PEG 3350, 0.2M Calcium chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K
|
Resolution 1.35 Å R-free 0.218 |
| 2RGC Crystal structure of H-RasQ61V-GppNHp Deposited 2007-10-03 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
Fragment：Residues 1-166
|
Mutation：Q61V | MG MAGNESIUM ION × 2 CA CALCIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；291 K；20 % PEG 3350, 0.2 M Calcium chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K
|
Resolution 1.60 Å R-free 0.213 |
| 2RGD Crystal structure of H-RasQ61L-GppNHp Deposited 2007-10-03 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
Fragment：Residues 1-166
|
Mutation：Q61L | CA CALCIUM ION × 1 MG MAGNESIUM ION × 2 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；291 K；20 % PEG 3350, 0.2M Calcium chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K
|
Resolution 2.00 Å R-free 0.220 |
| 2RGE Crystal structure of H-Ras-GppNHp Deposited 2007-10-03 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
Fragment：Residues 1-166
|
Not recorded | CA CALCIUM ION × 1 MG MAGNESIUM ION × 2 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；291 K；20% PEG 3350, 0.2M Calcium chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K
|
Resolution 1.40 Å R-free 0.219 |
| 2RGG Crystal structure of H-RasQ61I-GppNHp, trigonal crystal form Deposited 2007-10-03 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
Fragment：Residues 1-166
|
Mutation：Q61I | MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；291 K；20 % PEG 3350, 0.2 M Calcium chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K
|
Resolution 1.45 Å R-free 0.234 |
| 2UZI Crystal structure of HRAS(G12V) - anti-RAS Fv complex Deposited 2007-04-27 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain R
1–166（166 aa）
Fragment：RESIDUES 1-166
|
Mutation：YES | ZN ZINC ION × 5 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
PROTEIN WAS CRYSTALLIZED FROM 17-18 % PEG3350, 400 MM ZINC ACETATE, 100 MM SODIUM CACODYLATE, PH 5.8, 0.03 % DICHLOROMETHANE
|
Resolution 2.00 Å R-free 0.271 |
| 2VH5 CRYSTAL STRUCTURE OF HRAS(G12V) - ANTI-RAS FV (disulfide free mutant) COMPLEX Deposited 2007-11-19 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain R
1–166（166 aa）
Fragment：RESIDUES 1-166
|
Mutation：YES | ZN ZINC ION × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 5.8；PROTEIN WAS CRYSTALLIZED FROM 17-18 % PEG3350, 320 MM ZINC ACETATE, 100 MM SODIUM CACODYLATE, PH 5.8, 0.03 % DICHLOROMETHANE
|
Resolution 2.70 Å R-free 0.291 |
| 2X1V Crystal Structure of the activating H-Ras I163F mutant in Costello Syndrome, bound to MG-GDP Deposited 2010-01-04 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
Fragment：G-DOMAIN, RESIDUES 1-166
|
Mutation：YES | GDP GUANOSINE-5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7.6；18% POLYETHYLENE GLYCOL (PEG) 6000, 100 MM HEPES PH 7.4, 150 MM MGCL2.
|
Resolution 1.70 Å R-free 0.227 |
| 3DDC Crystal Structure of NORE1A in Complex with RAS Deposited 2008-06-05 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–166（166 aa）
Fragment：UNP residues 1-166
|
Mutation：D30E, E31K | MG MAGNESIUM ION × 2 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.5；293 K；100MM C2H3NAO2, 20% PEG 2000, 250MM (NH4)2SO4, 10MM DTE, pH 4.50, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.80 Å R-free 0.230 |
| 3DDC Crystal Structure of NORE1A in Complex with RAS Deposited 2008-06-05 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–166（166 aa）
Fragment：UNP residues 1-166
|
Mutation：D30E, E31K | MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.5；293 K；100MM C2H3NAO2, 20% PEG 2000, 250MM (NH4)2SO4, 10MM DTE, pH 4.50, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.80 Å R-free 0.230 |
| 3I3S Crystal Structure of H-Ras with Thr50 replaced by Isoleucine Deposited 2009-06-30 | Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain R
1–166（166 aa）
Fragment：UNP residues 1-166
|
Mutation：T50I | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 3 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
hanging drop；pH 7.5；285 K；17% PEG 6000, 200 mM CaCl2, pH 7.5, hanging drop, temperature 285K
|
Resolution 1.36 Å R-free 0.178 |
| 3I3S Crystal Structure of H-Ras with Thr50 replaced by Isoleucine Deposited 2009-06-30 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain R
1–166（166 aa）
Fragment：UNP residues 1-166
|
Mutation：T50I | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 6 MG MAGNESIUM ION × 18 CA CALCIUM ION × 6 |
X-RAY DIFFRACTION
X-ray crystallization conditions
hanging drop；pH 7.5；285 K；17% PEG 6000, 200 mM CaCl2, pH 7.5, hanging drop, temperature 285K
|
Resolution 1.36 Å R-free 0.178 |
| 3I3S Crystal Structure of H-Ras with Thr50 replaced by Isoleucine Deposited 2009-06-30 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 3 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain R
1–166（166 aa）
Fragment：UNP residues 1-166
|
Mutation：T50I | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 MG MAGNESIUM ION × 6 CA CALCIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
hanging drop；pH 7.5；285 K；17% PEG 6000, 200 mM CaCl2, pH 7.5, hanging drop, temperature 285K
|
Resolution 1.36 Å R-free 0.178 |
| 3I3S Crystal Structure of H-Ras with Thr50 replaced by Isoleucine Deposited 2009-06-30 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 4 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain R
1–166（166 aa）
Fragment：UNP residues 1-166
|
Mutation：T50I | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 MG MAGNESIUM ION × 6 CA CALCIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
hanging drop；pH 7.5；285 K；17% PEG 6000, 200 mM CaCl2, pH 7.5, hanging drop, temperature 285K
|
Resolution 1.36 Å R-free 0.178 |
| 3K8Y Allosteric modulation of H-Ras GTPase Deposited 2009-10-15 | Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
Fragment：UNP residues 1-166
|
Not recorded | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 CA CALCIUM ION × 2 MG MAGNESIUM ION × 2 ACT ACETATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；291.15 K；0.2M Calcium acetate, 20% PEG 3350, 0.05% n-Octyl-B-D-glucopyranoside, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 291.15K
|
Resolution 1.30 Å R-free 0.227 |
| 3K9L Allosteric modulation of H-Ras GTPase Deposited 2009-10-15 | Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
Fragment：UNP residues 1-166
|
Mutation：Y32F | CA CALCIUM ION × 1 MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；291.15 K；0.2M Calcium Acetate, 5 mM MgCl2, 10 mM DTT, 20% PEG 3350., pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291.15K
|
Resolution 1.80 Å R-free 0.271 |
| 3K9L Allosteric modulation of H-Ras GTPase Deposited 2009-10-15 | Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
1–166（166 aa）
Fragment：UNP residues 1-166
|
Mutation：Y32F | CA CALCIUM ION × 1 MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；291.15 K；0.2M Calcium Acetate, 5 mM MgCl2, 10 mM DTT, 20% PEG 3350., pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291.15K
|
Resolution 1.80 Å R-free 0.271 |
| 3K9L Allosteric modulation of H-Ras GTPase Deposited 2009-10-15 | Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
1–166（166 aa）
Fragment：UNP residues 1-166
|
Mutation：Y32F | MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；291.15 K；0.2M Calcium Acetate, 5 mM MgCl2, 10 mM DTT, 20% PEG 3350., pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291.15K
|
Resolution 1.80 Å R-free 0.271 |
| 3K9N Allosteric modulation of H-Ras GTPase Deposited 2009-10-16 | Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
Fragment：UNP residues 1-166
|
Mutation：Y32F | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 CA CALCIUM ION × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；291.5 K；0.2M Calcium Acetate, 20 % PEG 3350, 5 mM MgCl2, 10 mM DTT, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291.5K
|
Resolution 2.00 Å R-free 0.277 |
| 3KKM Crystal structure of H-Ras T35S in complex with GppNHp Deposited 2009-11-06 | Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
Fragment：G domain, UNP residues 1-166
|
Mutation：T35S | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 SO4 SULFATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；Tris-HCl, Ammonium Sulfate, DMSO, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 1.70 Å R-free 0.232 |
| 3KKN Crystal structure of H-Ras T35S in complex with GppNHp Deposited 2009-11-06 | Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
Fragment：G domain, UNP residues 1-166
|
Mutation：T35S | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；MES, Ammonium Sulfate, PEG-MME-5000, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 2.09 Å R-free 0.242 |
| 3KUD Complex of Ras-GDP with RafRBD(A85K) Deposited 2009-11-27 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–166（166 aa）
Fragment：UNP residues 1-166
|
Not recorded | GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；298 K；1.3M Na-Malonat pH 6.0, 100 mM MES pH 6.1, 4% Betaine, 2% Sarcosine, 2% N,N-dimethylglycine, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.15 Å R-free 0.264 |
| 3L8Y Complex of Ras with cyclen Deposited 2010-01-04 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
Fragment：residues 1-166
|
Not recorded | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 CA CALCIUM ION × 4 YCN 1,4,7,10-tetraazacyclododecane × 1 ZN ZINC ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；285 K；17% PEG6000, 200mM CaCl2, 30mM TRIS/HCL, 5mM MgCl2, 2mM DTE, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 285K
|
Resolution 2.02 Å R-free 0.238 |
| 3L8Z H-Ras wildtype new crystal form Deposited 2010-01-04 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
Fragment：residues 1-166
|
Not recorded | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 CA CALCIUM ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；285 K；17% PEG6000, 200mM CaCl2, 30mM TRIS/HCL, 5mM MgCl2, 2mM DTE, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 285K
|
Resolution 1.44 Å R-free 0.220 |
| 3LBH Ras soaked in Calcium Acetate Deposited 2010-01-08 | Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
Fragment：UNP residues 1-166
|
Not recorded | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 CA CALCIUM ION × 2 MG MAGNESIUM ION × 2 ACT ACETATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；291.15 K；200 mM Calcium Acetate, 20 % PEG 3350, 0.05% n-Octylglucopyranoside, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 291.15K
|
Resolution 1.85 Å R-free 0.209 |
| 3LBH Ras soaked in Calcium Acetate Deposited 2010-01-08 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
1–166（166 aa）
Fragment：UNP residues 1-166
|
Not recorded | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 3 CA CALCIUM ION × 6 MG MAGNESIUM ION × 6 ACT ACETATE ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；291.15 K；200 mM Calcium Acetate, 20 % PEG 3350, 0.05% n-Octylglucopyranoside, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 291.15K
|
Resolution 1.85 Å R-free 0.209 |
| 3LBI Ras soaked in Magnesium Acetate and back soaked in Calcium Acetate Deposited 2010-01-08 | Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
Fragment：UNP residues 1-166
|
Not recorded | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 CA CALCIUM ION × 2 MG MAGNESIUM ION × 2 ACT ACETATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；291.15 K；200 mM Calcium Acetate, 20 % PEG 3350, 0.05% n-Octylglucopyranoside, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 291.15K
|
Resolution 2.09 Å R-free 0.217 |
| 3LBI Ras soaked in Magnesium Acetate and back soaked in Calcium Acetate Deposited 2010-01-08 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
1–166（166 aa）
Fragment：UNP residues 1-166
|
Not recorded | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 3 CA CALCIUM ION × 6 MG MAGNESIUM ION × 6 ACT ACETATE ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；291.15 K；200 mM Calcium Acetate, 20 % PEG 3350, 0.05% n-Octylglucopyranoside, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 291.15K
|
Resolution 2.09 Å R-free 0.217 |
| 3LBN Ras soaked in Magnesium Acetate Deposited 2010-01-08 | Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
Fragment：UNP residues 1-166
|
Not recorded | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 CA CALCIUM ION × 1 MG MAGNESIUM ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；291.15 K；200 mM Calcium Acetate, 20 % PEG 3350, 0.05% n-Octylglucopyranoside, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 291.15K
|
Resolution 1.86 Å R-free 0.224 |
| 3LBN Ras soaked in Magnesium Acetate Deposited 2010-01-08 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
1–166（166 aa）
Fragment：UNP residues 1-166
|
Not recorded | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 3 CA CALCIUM ION × 3 MG MAGNESIUM ION × 9 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；291.15 K；200 mM Calcium Acetate, 20 % PEG 3350, 0.05% n-Octylglucopyranoside, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 291.15K
|
Resolution 1.86 Å R-free 0.224 |
| 3LO5 Crystal Structure of the dominant negative S17N mutant of Ras Deposited 2010-02-03 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
Fragment：H-Ras (UNP residues 1-166)
|
Mutation：S17N | GDP GUANOSINE-5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；15% (v/v) PEG400, 13% (w/v) PEG8000, 0.2 M calcium acetate, and 0.1 M Tris-HCl pH = 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.57 Å R-free 0.296 |
| 3LO5 Crystal Structure of the dominant negative S17N mutant of Ras Deposited 2010-02-03 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
1–166（166 aa）
Fragment：H-Ras (UNP residues 1-166)
|
Mutation：S17N | GDP GUANOSINE-5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；15% (v/v) PEG400, 13% (w/v) PEG8000, 0.2 M calcium acetate, and 0.1 M Tris-HCl pH = 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.57 Å R-free 0.296 |
| 3LO5 Crystal Structure of the dominant negative S17N mutant of Ras Deposited 2010-02-03 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain E
1–166（166 aa）
Fragment：H-Ras (UNP residues 1-166)
|
Mutation：S17N | GDP GUANOSINE-5'-DIPHOSPHATE × 1 CA CALCIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；15% (v/v) PEG400, 13% (w/v) PEG8000, 0.2 M calcium acetate, and 0.1 M Tris-HCl pH = 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.57 Å R-free 0.296 |
| 3OIU H-RasQ61L with allosteric switch in the "on" state Deposited 2010-08-20 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
|
Mutation：Q61L | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 CA CALCIUM ION × 3 MG MAGNESIUM ION × 2 ACT ACETATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
sitting drop；pH 7.5；298 K；365mM Calcium Acetate, 24 % PEG 3350, pH 7.5, sitting drop, temperature 298K
|
Resolution 1.32 Å R-free 0.192 |
| 3OIU H-RasQ61L with allosteric switch in the "on" state Deposited 2010-08-20 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
1–166（166 aa）
|
Mutation：Q61L | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 3 CA CALCIUM ION × 9 MG MAGNESIUM ION × 6 ACT ACETATE ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
sitting drop；pH 7.5；298 K；365mM Calcium Acetate, 24 % PEG 3350, pH 7.5, sitting drop, temperature 298K
|
Resolution 1.32 Å R-free 0.192 |
| 3OIV H-RasG12V with allosteric switch in the "off" state Deposited 2010-08-20 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
|
Mutation：G12V | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 CA CALCIUM ION × 1 MG MAGNESIUM ION × 3 DTT 2,3-DIHYDROXY-1,4-DITHIOBUTANE × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
sitting drop；pH 7.5；298 K；200 Mm Calcium Chloride, 20 % PEG 3350, pH 7.5, sitting drop, temperature 298K
|
Resolution 1.84 Å R-free 0.198 |
| 3OIV H-RasG12V with allosteric switch in the "off" state Deposited 2010-08-20 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
1–166（166 aa）
|
Mutation：G12V | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 3 CA CALCIUM ION × 3 MG MAGNESIUM ION × 9 DTT 2,3-DIHYDROXY-1,4-DITHIOBUTANE × 6 |
X-RAY DIFFRACTION
X-ray crystallization conditions
sitting drop；pH 7.5；298 K；200 Mm Calcium Chloride, 20 % PEG 3350, pH 7.5, sitting drop, temperature 298K
|
Resolution 1.84 Å R-free 0.198 |
| 3OIW H-RasG12V with allosteric switch in the "on" state Deposited 2010-08-20 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
|
Mutation：G12V | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 CA CALCIUM ION × 3 MG MAGNESIUM ION × 2 ACT ACETATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
sitting drop；pH 7.5；298 K；200 Mm Calcium Acetate, 20 % PEG 3350, pH 7.5, sitting drop, temperature 298K
|
Resolution 1.30 Å R-free 0.210 |
| 3OIW H-RasG12V with allosteric switch in the "on" state Deposited 2010-08-20 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
1–166（166 aa）
|
Mutation：G12V | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 3 CA CALCIUM ION × 9 MG MAGNESIUM ION × 6 ACT ACETATE ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
sitting drop；pH 7.5；298 K；200 Mm Calcium Acetate, 20 % PEG 3350, pH 7.5, sitting drop, temperature 298K
|
Resolution 1.30 Å R-free 0.210 |
| 3RRY H-Ras crosslinked control, soaked in aqueous solution: one of 10 in MSCS set Deposited 2011-05-02 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
|
Not recorded | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 CA CALCIUM ION × 2 MG MAGNESIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
hanging drop；pH 7.5；298 K；20 % PEG 3350, 200mM Calcium Chloride, pH 7.5, hanging drop, temperature 298K
|
Resolution 1.60 Å R-free 0.201 |
| 3RRZ H-Ras in 70% glycerol: one of 10 in MSCS set Deposited 2011-05-02 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
|
Not recorded | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 GOL GLYCEROL × 8 MG MAGNESIUM ION × 2 CA CALCIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
hanging drop；pH 7.5；298 K；20 % PEG 3350, 200mM Calcium Chloride, pH 7.5, hanging drop, temperature 298K
|
Resolution 1.60 Å R-free 0.205 |
| 3RS0 H-Ras soaked in neat cyclopentanol: one of 10 in MSCS set Deposited 2011-05-02 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
|
Not recorded | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 YEG cyclopentanol × 2 CA CALCIUM ION × 2 MG MAGNESIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
hanging drop；pH 7.5；298 K；20 % PEG 3350, 200mM Calcium Chloride, pH 7.5, hanging drop, temperature 298K
|
Resolution 1.40 Å R-free 0.185 |
| 3RS2 H-Ras soaked in 50% 2,2,2-trifluoroethanol: one of 10 in MSCS set Deposited 2011-05-02 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
|
Not recorded | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ETF TRIFLUOROETHANOL × 2 CA CALCIUM ION × 2 MG MAGNESIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
hanging drop；pH 7.5；298 K；20 % PEG 3350, 200mM Calcium Chloride, pH 7.5, hanging drop, temperature 298K
|
Resolution 1.84 Å R-free 0.203 |
| 3RS3 H-Ras soaked in neat hexane: 1 of 10 in MSCS set Deposited 2011-05-02 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
|
Not recorded | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 HEX HEXANE × 2 CA CALCIUM ION × 3 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
hanging drop；pH 7.5；298 K；20 % PEG 3350, 200mM Calcium Chloride, pH 7.5, hanging drop, temperature 298K
|
Resolution 1.52 Å R-free 0.207 |
| 3RS4 H-Ras soaked in 60% 1,6-hexanediol: 1 of 10 in MSCS set Deposited 2011-05-02 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
|
Not recorded | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 HEZ HEXANE-1,6-DIOL × 1 MG MAGNESIUM ION × 2 CA CALCIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
hanging drop；pH 7.5；298 K；20 % PEG 3350, 200mM Calcium Chloride, pH 7.5, hanging drop, temperature 298K
|
Resolution 1.70 Å R-free 0.198 |
| 3RS5 H-Ras soaked in 55% dimethylformamide: 1 of 10 in MSCS set Deposited 2011-05-02 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
|
Not recorded | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 DMF DIMETHYLFORMAMIDE × 4 CA CALCIUM ION × 2 MG MAGNESIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
hanging drop；pH 7.5；298 K；20 % PEG 3350, 200mM Calcium Chloride, pH 7.5, hanging drop, temperature 298K
|
Resolution 1.68 Å R-free 0.182 |
| 3RS7 H-Ras soaked in 50% isopropanol: 1 of 10 in MSCS set Deposited 2011-05-02 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
|
Not recorded | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 CA CALCIUM ION × 2 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
hanging drop；pH 7.5；298 K；20 % PEG 3350, 200mM Calcium Chloride, pH 7.5, hanging drop, temperature 298K
|
Resolution 1.70 Å R-free 0.208 |
| 3RSL H-Ras soaked in 90% R,S,R-bisfuranol: one of 10 in MSCS set Deposited 2011-05-02 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
|
Not recorded | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 RSF (3R,3aS,6aR)-hexahydrofuro[2,3-b]furan-3-ol × 13 MG MAGNESIUM ION × 1 CA CALCIUM ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
hanging drop；pH 7.5；298 K；20 % PEG 3350, 200mM Calcium Chloride, pH 7.5, hanging drop, temperature 298K
|
Resolution 1.70 Å R-free 0.220 |
| 3RSO H-Ras soaked in 20% S,R,S-bisfuranol: 1 of 10 in MSCS set Deposited 2011-05-02 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
|
Not recorded | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 CA CALCIUM ION × 2 MG MAGNESIUM ION × 1 RSG (3S,3aR,6aS)-hexahydrofuro[2,3-b]furan-3-ol × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
hanging drop；pH 7.5；298 K；20 % PEG 3350, 200mM Calcium Chloride, pH 7.5, hanging drop, temperature 298K
|
Resolution 1.60 Å R-free 0.199 |
| 3TGP Room temperature H-ras Deposited 2011-08-17 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
|
Not recorded | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
MICROBATCH；pH 7.6；291 K；Protein was crystallized from 28 % PEG 400, 10mM MgCl 264 mM TRIS/HCl, PH 7.6, pH 7.60, micro-batch, temperature 291.0K
|
Resolution 1.31 Å R-free 0.213 |
| 421P THREE-DIMENSIONAL STRUCTURES OF H-RAS P21 MUTANTS: MOLECULAR BASIS FOR THEIR INABILITY TO FUNCTION AS SIGNAL SWITCH MOLECULES Deposited 1991-06-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–166（166 aa）
|
Mutation：G12R | MG MAGNESIUM ION × 2 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.20 Å |
| 4DLR H-Ras PEG 400/Ca(OAc)2, ordered off Deposited 2012-02-06 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
|
Not recorded | CA CALCIUM ION × 3 MG MAGNESIUM ION × 3 DTU (2R,3S)-1,4-DIMERCAPTOBUTANE-2,3-DIOL × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；298 K；20% PEG 3350, 200 mM Ca Acetate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 1.32 Å R-free 0.186 |
| 4DLR H-Ras PEG 400/Ca(OAc)2, ordered off Deposited 2012-02-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain A
1–166（166 aa）
|
Not recorded | CA CALCIUM ION × 18 MG MAGNESIUM ION × 18 DTU (2R,3S)-1,4-DIMERCAPTOBUTANE-2,3-DIOL × 6 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 6 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；298 K；20% PEG 3350, 200 mM Ca Acetate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 1.32 Å R-free 0.186 |
| 4DLS H-Ras Set 1 CaCl2 'Mixed' Deposited 2012-02-06 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
|
Not recorded | CA CALCIUM ION × 2 MG MAGNESIUM ION × 2 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；298 K；20% PEG 3350, 200 mM CaCl2, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 1.82 Å R-free 0.194 |
| 4DLS H-Ras Set 1 CaCl2 'Mixed' Deposited 2012-02-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain A
1–166（166 aa）
|
Not recorded | CA CALCIUM ION × 12 MG MAGNESIUM ION × 12 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 6 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；298 K；20% PEG 3350, 200 mM CaCl2, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 1.82 Å R-free 0.194 |
| 4DLT H-Ras Set 2 Ca(OAc)2, on Deposited 2012-02-06 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
|
Not recorded | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 CA CALCIUM ION × 2 MG MAGNESIUM ION × 2 ACT ACETATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；298 K；20% PEG 3350, 200 mM Ca Acetate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 1.70 Å R-free 0.204 |
| 4DLT H-Ras Set 2 Ca(OAc)2, on Deposited 2012-02-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
1–166（166 aa）
|
Not recorded | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 3 CA CALCIUM ION × 6 MG MAGNESIUM ION × 6 ACT ACETATE ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；298 K；20% PEG 3350, 200 mM Ca Acetate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 1.70 Å R-free 0.204 |
| 4DLU H-Ras Set 1 Ca(OAc)2, on Deposited 2012-02-06 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
|
Not recorded | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 CA CALCIUM ION × 2 MG MAGNESIUM ION × 2 ACT ACETATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；298 K；20% PEG 3350, 200 mM CaCl2, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 1.60 Å R-free 0.203 |
| 4DLU H-Ras Set 1 Ca(OAc)2, on Deposited 2012-02-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
1–166（166 aa）
|
Not recorded | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 3 CA CALCIUM ION × 6 MG MAGNESIUM ION × 6 ACT ACETATE ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；298 K；20% PEG 3350, 200 mM CaCl2, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 1.60 Å R-free 0.203 |
| 4DLV H-Ras Set 2 CaCl2/DTT, ordered off Deposited 2012-02-06 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
|
Not recorded | CA CALCIUM ION × 1 MG MAGNESIUM ION × 3 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 DTT 2,3-DIHYDROXY-1,4-DITHIOBUTANE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
sitting drop；pH 7.5；298 K；20% PEG 3350, 200 mM Ca Acetate, pH 7.5, sitting drop, temperature 298K
|
Resolution 1.57 Å R-free 0.211 |
| 4DLV H-Ras Set 2 CaCl2/DTT, ordered off Deposited 2012-02-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain A
1–166（166 aa）
|
Not recorded | CA CALCIUM ION × 6 MG MAGNESIUM ION × 18 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 6 DTT 2,3-DIHYDROXY-1,4-DITHIOBUTANE × 6 |
X-RAY DIFFRACTION
X-ray crystallization conditions
sitting drop；pH 7.5；298 K；20% PEG 3350, 200 mM Ca Acetate, pH 7.5, sitting drop, temperature 298K
|
Resolution 1.57 Å R-free 0.211 |
| 4DLW H-Ras Set 2 Ca(OAc)2/DTT, on Deposited 2012-02-06 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
|
Not recorded | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 CA CALCIUM ION × 2 MG MAGNESIUM ION × 2 ACT ACETATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；298 K；20% PEG 3350, 200 mM Ca Acetate, pH 7.5, temperature 298K, VAPOR DIFFUSION, SITTING DROP
|
Resolution 1.72 Å R-free 0.202 |
| 4DLW H-Ras Set 2 Ca(OAc)2/DTT, on Deposited 2012-02-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
1–166（166 aa）
|
Not recorded | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 3 CA CALCIUM ION × 6 MG MAGNESIUM ION × 6 ACT ACETATE ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；298 K；20% PEG 3350, 200 mM Ca Acetate, pH 7.5, temperature 298K, VAPOR DIFFUSION, SITTING DROP
|
Resolution 1.72 Å R-free 0.202 |
| 4DLX H-Ras Set 1 CaCl2/DTE, ordered off Deposited 2012-02-06 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
|
Not recorded | CA CALCIUM ION × 1 MG MAGNESIUM ION × 3 DTU (2R,3S)-1,4-DIMERCAPTOBUTANE-2,3-DIOL × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；298 K；20% PEG 3350 200 mM CaCl2, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 1.73 Å R-free 0.198 |
| 4DLX H-Ras Set 1 CaCl2/DTE, ordered off Deposited 2012-02-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain A
1–166（166 aa）
|
Not recorded | CA CALCIUM ION × 6 MG MAGNESIUM ION × 18 DTU (2R,3S)-1,4-DIMERCAPTOBUTANE-2,3-DIOL × 6 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 6 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；298 K；20% PEG 3350 200 mM CaCl2, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 1.73 Å R-free 0.198 |
| 4DLY Set 1 CaCl2/DTT, ordered off Deposited 2012-02-06 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
|
Not recorded | CA CALCIUM ION × 3 MG MAGNESIUM ION × 2 DTT 2,3-DIHYDROXY-1,4-DITHIOBUTANE × 2 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；298 K；20% PEG 3350, 200 mM CaCl2, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 1.57 Å R-free 0.179 |
| 4DLY Set 1 CaCl2/DTT, ordered off Deposited 2012-02-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain A
1–166（166 aa）
|
Not recorded | CA CALCIUM ION × 18 MG MAGNESIUM ION × 12 DTT 2,3-DIHYDROXY-1,4-DITHIOBUTANE × 12 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 6 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；298 K；20% PEG 3350, 200 mM CaCl2, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 1.57 Å R-free 0.179 |
| 4DLZ H-Ras Set 2 Ca(OAc)2/DTE, ordered off Deposited 2012-02-06 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
|
Not recorded | CA CALCIUM ION × 2 MG MAGNESIUM ION × 3 DTU (2R,3S)-1,4-DIMERCAPTOBUTANE-2,3-DIOL × 2 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；298 K；20% PEG 3350, 200 mM Ca Acetate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 1.66 Å R-free 0.184 |
| 4DLZ H-Ras Set 2 Ca(OAc)2/DTE, ordered off Deposited 2012-02-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain A
1–166（166 aa）
|
Not recorded | CA CALCIUM ION × 12 MG MAGNESIUM ION × 18 DTU (2R,3S)-1,4-DIMERCAPTOBUTANE-2,3-DIOL × 12 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 6 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；298 K；20% PEG 3350, 200 mM Ca Acetate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 1.66 Å R-free 0.184 |
| 4EFL Crystal structure of H-Ras WT in complex with GppNHp (state 1) Deposited 2012-03-30 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
Fragment：G domain, UNP residues 1-166
|
Not recorded | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；0.1M MES, 0.2M ammonium sulfate, 30% (w/v) PEG 5000 MME, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 1.90 Å R-free 0.204 |
| 4EFM Crystal structure of H-Ras G12V in complex with GppNHp (state 1) Deposited 2012-03-30 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
Fragment：G domain, UNP residues 1-166
|
Mutation：G12V | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；0.1M MES, 0.2M ammonium sulfate, 30% (w/v) PEG 5000 MME, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 1.90 Å R-free 0.221 |
| 4EFN Crystal structure of H-Ras Q61L in complex with GppNHp (state 1) Deposited 2012-03-30 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
Fragment：G domain, UNP residues 1-166
|
Mutation：Q61L | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；0.1M MES, 0.2M ammonium sulfate, 30% (w/v) PEG 5000 MME, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 2.30 Å R-free 0.237 |
| 4G0N Crystal Structure of wt H-Ras-GppNHp bound to the RBD of Raf Kinase Deposited 2012-07-09 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–166（166 aa）
Fragment：UNP residues 1-166
|
Not recorded | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 CA CALCIUM ION × 2 MG MAGNESIUM ION × 1 ACT ACETATE ION × 1 DTU (2R,3S)-1,4-DIMERCAPTOBUTANE-2,3-DIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.8；291 K；Protein solution: 10 - 16 mg/mL, 50 mM HEPES, pH 7.2, 50 mM NaCl, 10mM MgCl2 5% Glycerol, 1mM DTE, 10 M ZnCl2
Reservoir solution:200mM calcium acetate, 100mM sodium cacodylate pH 6.5, 18% PEG 8000.
Drop: 1uL protein, 1uL reservoir, VAPOR DIFFUSION, SITTING DROP, temperature 291K
|
Resolution 2.45 Å R-free 0.229 |
| 4G3X Crystal Structure of Q61L H-Ras-GppNHp bound to the RBD of Raf Kinase Deposited 2012-07-15 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–166（166 aa）
|
Mutation：Q61L | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；291 K；Protein solution: 10 - 18 mg/mL, 50 mM HEPES, pH 7.2, 50 mM NaCl, 10mM MgCl2 5% Glycerol, 1mM DTE, 10 M ZnCl2 Reservoir solution:200mM calcium acetate, 100mM sodium cacodylate pH 6.5, 18% PEG 8000. Drop: 3uL protein, 3uL reservoir , VAPOR DIFFUSION, HANGING DROP, temperature 291K
|
Resolution 3.25 Å R-free 0.271 |
| 4K81 Crystal structure of the Grb14 RA and PH domains in complex with GTP-loaded H-Ras Deposited 2013-04-17 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
1–166（166 aa）
Fragment：GTPase domain, UNP residues 1-166
|
Mutation：G12V | GOL GLYCEROL × 5 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.9；315 K；14% (w/v) PEG 3350, 100 mM MES, pH 5.9, 200 mM MgCl2, 2% glycerol, and 3% glucose, VAPOR DIFFUSION, HANGING DROP, temperature 315K
|
Resolution 2.40 Å R-free 0.277 |
| 4K81 Crystal structure of the Grb14 RA and PH domains in complex with GTP-loaded H-Ras Deposited 2013-04-17 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain D
1–166（166 aa）
Fragment：GTPase domain, UNP residues 1-166
|
Mutation：G12V | GOL GLYCEROL × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.9；315 K；14% (w/v) PEG 3350, 100 mM MES, pH 5.9, 200 mM MgCl2, 2% glycerol, and 3% glucose, VAPOR DIFFUSION, HANGING DROP, temperature 315K
|
Resolution 2.40 Å R-free 0.277 |
| 4K81 Crystal structure of the Grb14 RA and PH domains in complex with GTP-loaded H-Ras Deposited 2013-04-17 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain F
1–166（166 aa）
Fragment：GTPase domain, UNP residues 1-166
|
Mutation：G12V | GOL GLYCEROL × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.9；315 K；14% (w/v) PEG 3350, 100 mM MES, pH 5.9, 200 mM MgCl2, 2% glycerol, and 3% glucose, VAPOR DIFFUSION, HANGING DROP, temperature 315K
|
Resolution 2.40 Å R-free 0.277 |
| 4K81 Crystal structure of the Grb14 RA and PH domains in complex with GTP-loaded H-Ras Deposited 2013-04-17 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain H
1–166（166 aa）
Fragment：GTPase domain, UNP residues 1-166
|
Mutation：G12V | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.9；315 K；14% (w/v) PEG 3350, 100 mM MES, pH 5.9, 200 mM MgCl2, 2% glycerol, and 3% glucose, VAPOR DIFFUSION, HANGING DROP, temperature 315K
|
Resolution 2.40 Å R-free 0.277 |
| 4L9S Crystal Structure of H-Ras G12C, GDP-bound Deposited 2013-06-18 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
Fragment：GTPase domain, UNP residues 1-166
|
Mutation：G12C | GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 CA CALCIUM ION × 2 NA SODIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；17% PEG8000, 0.1M CaCl2, 0.1M TRIS, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.61 Å R-free 0.173 |
| 4L9W Crystal Structure of H-Ras G12C, GMPPNP-bound Deposited 2013-06-18 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–166（166 aa）
Fragment：GTPase domain, UNP residues 1-166
|
Mutation：G12C | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 MG MAGNESIUM ION × 2 CA CALCIUM ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；19% PEG3350, 0.2M CaCl2, pH 7.5, vapor diffusion, hanging drop, temperature 293K
|
Resolution 1.95 Å R-free 0.189 |
| 4L9W Crystal Structure of H-Ras G12C, GMPPNP-bound Deposited 2013-06-18 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–166（166 aa）
Fragment：GTPase domain, UNP residues 1-166
|
Mutation：G12C | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 MG MAGNESIUM ION × 2 CA CALCIUM ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；19% PEG3350, 0.2M CaCl2, pH 7.5, vapor diffusion, hanging drop, temperature 293K
|
Resolution 1.95 Å R-free 0.189 |
| 4NYI Approach for Targeting Ras with Small Molecules that Activate SOS-Mediated Nucleotide Exchange Deposited 2013-12-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain Q
1–166（166 aa）
Chain R
1–166（166 aa）
|
Mutation：Y64A | MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 2PX N-{1-[(5-methyl-1H-indol-3-yl)methyl]piperidin-4-yl}-L-tryptophanamide × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4；293 K；0.1 M sodium acetate, 2.0 M sodium formate, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.96 Å R-free 0.208 |
| 4NYJ Approach for Targeting Ras with Small Molecules that Activate SOS-Mediated Nucleotide Exchange Deposited 2013-12-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain Q
1–166（166 aa）
Chain R
1–166（166 aa）
|
Mutation：Y64A | MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 2PZ N-[1-(1H-indol-3-ylmethyl)piperidin-4-yl]glycinamide × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4；293 K；0.1 M sodium acetate, 2.0 M sodium formate, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.85 Å R-free 0.187 |
| 4NYM Approach for Targeting Ras with Small Molecules that Activate SOS-Mediated Nucleotide Exchange Deposited 2013-12-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain Q
1–166（166 aa）
Chain R
1–166（166 aa）
|
Mutation：Y64A | MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 RND N-[1-(1H-indol-3-ylmethyl)piperidin-4-yl]-L-tryptophanamide × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.5；293 K；0.1 M sodium acetate, 1.8 M sodium formate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 3.55 Å R-free 0.214 |
| 4Q21 MOLECULAR SWITCH FOR SIGNAL TRANSDUCTION: STRUCTURAL DIFFERENCES BETWEEN ACTIVE AND INACTIVE FORMS OF PROTOONCOGENIC RAS PROTEINS Deposited 1991-09-25 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–189（189 aa）
|
Not recorded | MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.00 Å |
| 4RSG Neutron crystal structure of Ras bound to the GTP analogue GppNHp Deposited 2014-11-07 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
|
Not recorded | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 |
NEUTRON DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；291 K；18mg/mL protein, 0.2M calcium acetate, 20% PEG 3350, 20mM HEPES, 50mM NaCl, 20mM MgCl2, 0.1% N-octy-B-D-glucopyranoside, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K
|
Resolution 1.91 Å R-free 0.287 |
| 4URU The crystal structure of H-Ras and SOS in complex with ligands Deposited 2014-07-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain R
1–166（166 aa）
Fragment：UNP RESIDUES 1-166
|
Not recorded | 6W2 4-METHOXY-N-(1,3-THIAZOL-2-YL)BENZENESULFONAMIDE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
3.2M SODIUM FORMATE, 2% DMSO
|
Resolution 2.83 Å R-free 0.237 |
| 4URV The crystal structure of H-Ras and SOS in complex with ligands Deposited 2014-07-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain R
1–166（166 aa）
Fragment：UNP RESIDUES 1-166
|
Not recorded | FMT FORMIC ACID × 2 UMK 4-(4-BROMOPHENYL)PIPERIDIN-4-OL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
3.2M SODIUM FORMATE, 2% DMSO
|
Resolution 2.58 Å R-free 0.232 |
| 4URW The crystal structure of H-Ras and SOS in complex with ligands Deposited 2014-07-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain R
1–166（166 aa）
Fragment：UNP RESIDUES 1-166
|
Not recorded | DXO 2-(2,6-DIMETHYLPHENYL)-4-(METHYLSULFANYL)-6-(PIPERAZIN-1-YL)-1,3,5-TRIAZINE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
3.2M SODIUM FORMATE, 2% DMSO
|
Resolution 2.76 Å R-free 0.230 |
| 4URX The crystal structure of H-Ras and SOS in complex with ligands Deposited 2014-07-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain R
1–166（166 aa）
Fragment：RESIDUES 1-166
|
Not recorded | FK1 6-bromo-1H-indole × 1 HXY 1-(4-bromobenzyl)pyrrolidine × 1 FMT FORMIC ACID × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.49 Å R-free 0.227 |
| 4URY The crystal structure of H-Ras and SOS in complex with ligands Deposited 2014-07-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain R
1–166（166 aa）
Fragment：RESIDUES 1-166
|
Not recorded | RV1 N-[(4-aminophenyl)sulfonyl]cyclopropanecarboxamide × 2 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.47 Å R-free 0.229 |
| 4URZ The crystal structure of H-Ras and SOS in complex with ligands Deposited 2014-07-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain R
1–166（166 aa）
Fragment：RESIDUES 1-166
|
Not recorded | VJP 1-[(4-aminophenyl)sulfonyl]piperidin-2-one × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.24 Å R-free 0.212 |
| 4US0 The crystal structure of H-Ras and SOS in complex with ligands Deposited 2014-07-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain R
1–166（166 aa）
Fragment：UNP RESIDUES 1-166
|
Not recorded | NEN 1-ETHYL-PYRROLIDINE-2,5-DIONE × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.17 Å R-free 0.212 |
| 4US1 The crystal structure of H-Ras and SOS in complex with ligands Deposited 2014-07-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain R
1–166（166 aa）
Fragment：UNP RESIDUES 1-166
|
Not recorded | L71 (3S)-3-[3-(aminomethyl)phenyl]-1-ethylpyrrolidine-2,5-dione × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.65 Å R-free 0.231 |
| 4US2 The crystal structure of H-Ras and SOS in complex with ligands Deposited 2014-07-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain R
1–166（166 aa）
Fragment：UNP RESIDUES 1-166
|
Not recorded | L7S 3-[(3R)-1-ethyl-2,5-dioxopyrrolidin-3-yl]benzamide × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.48 Å R-free 0.228 |
| 4XVQ H-Ras Y137E Deposited 2015-01-27 | Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
Fragment：UNP residues 1-166
|
Mutation：Y137E | MG MAGNESIUM ION × 2 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；291.15 K；152mM Ca(OAc)2, 24.8% PEG 3350, 4.8% stabilization buffer at pH 7.5.
|
Resolution 1.89 Å R-free 0.254 |
| 4XVR H-Ras Y137F Deposited 2015-01-27 | Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
Fragment：UNP residues 1-166
|
Mutation：Y137F | MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；291.15 K；139mM Ca(OAc)2, 22.6% PEG 3350, 13% stabilization buffer at pH 7.5
|
Resolution 2.03 Å R-free 0.286 |
| 521P THREE-DIMENSIONAL STRUCTURES OF H-RAS P21 MUTANTS: MOLECULAR BASIS FOR THEIR INABILITY TO FUNCTION AS SIGNAL SWITCH MOLECULES Deposited 1991-06-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–166（166 aa）
|
Mutation：G12V, A59T | MG MAGNESIUM ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.60 Å |
| 5B2Z H-Ras WT in complex with GppNHp (state 2*) before structural transition by humidity control Deposited 2016-02-07 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
|
Not recorded | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 CA CALCIUM ION × 2 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 4.5；293 K；PEG400, SODIUM ACETATE, CALCIUM ACETATE, MAGNESIUM CHLORIDE
|
Resolution 1.56 Å R-free 0.178 |
| 5B30 H-Ras WT in complex with GppNHp (state 1) after structural transition by humidity control Deposited 2016-02-08 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
|
Not recorded | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 CA CALCIUM ION × 2 NA SODIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 4.5；293 K；PEG400, SODIUM ACETATE, CALCIUM ACETATE, MAGNESIUM CHLORIDE
|
Resolution 1.60 Å R-free 0.196 |
| 5E95 Crystal Structure of Mb(NS1)/H-Ras Complex Deposited 2015-10-14 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–166（166 aa）
Fragment：UNP residues 1-166
|
Not recorded | GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.2；292 K；0.2M Ammonium Fluoride, 20% PEG 2250
|
Resolution 1.40 Å R-free 0.192 |
| 5P21 REFINED CRYSTAL STRUCTURE OF THE TRIPHOSPHATE CONFORMATION OF H-RAS P21 AT 1.35 ANGSTROMS RESOLUTION: IMPLICATIONS FOR THE MECHANISM OF GTP HYDROLYSIS Deposited 1990-04-30 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–166（166 aa）
|
Not recorded | MG MAGNESIUM ION × 2 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.35 Å |
| 5VBE Crystal Structure of Small Molecule Disulfide 2C07 Bound to H-Ras M72C GDP Deposited 2017-03-29 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
|
Mutation：M72C | GDP GUANOSINE-5'-DIPHOSPHATE × 1 92V 1-(4-methoxyphenyl)-N-(3-sulfanylpropyl)-5-(trifluoromethyl)-1H-pyrazole-4-carboxamide × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.7；293 K；22% PEG8000
.1M Tris HCl (pH 7.7)
.1 M CaCl2
|
Resolution 1.57 Å R-free 0.201 |
| 5VBZ Crystal Structure of Small Molecule Disulfide 2C07 Bound to H-Ras M72C GppNHp Deposited 2017-03-30 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
|
Mutation：M72C | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.6；298 K；32% PEG4000
.1 M Na Cacodylate (pH 6.6)
.2 M CaCl2
|
Resolution 2.20 Å R-free 0.253 |
| 5VBZ Crystal Structure of Small Molecule Disulfide 2C07 Bound to H-Ras M72C GppNHp Deposited 2017-03-30 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
1–166（166 aa）
|
Mutation：M72C | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.6；298 K；32% PEG4000
.1 M Na Cacodylate (pH 6.6)
.2 M CaCl2
|
Resolution 2.20 Å R-free 0.253 |
| 5VBZ Crystal Structure of Small Molecule Disulfide 2C07 Bound to H-Ras M72C GppNHp Deposited 2017-03-30 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
1–166（166 aa）
|
Mutation：M72C | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 92V 1-(4-methoxyphenyl)-N-(3-sulfanylpropyl)-5-(trifluoromethyl)-1H-pyrazole-4-carboxamide × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.6；298 K；32% PEG4000
.1 M Na Cacodylate (pH 6.6)
.2 M CaCl2
|
Resolution 2.20 Å R-free 0.253 |
| 5WDO H-Ras bound to GMP-PNP at 277K Deposited 2017-07-05 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
|
Not recorded | CA CALCIUM ION × 1 MG MAGNESIUM ION × 2 NA SODIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；16% PEG3350, 100 mM calcium acetate, pH 7.5
|
Resolution 1.65 Å R-free 0.168 |
| 5WDP H-Ras mutant L120A bound to GMP-PNP at 277K Deposited 2017-07-05 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
|
Mutation：L120A | CA CALCIUM ION × 2 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6；293 K；17% PEG8000, 200 mM calcium acetate, 100 mM MES/NaOH, pH 6.0
|
Resolution 1.35 Å R-free 0.154 |
| 5WDQ H-Ras mutant L120A bound to GMP-PNP at 100K Deposited 2017-07-05 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
|
Mutation：L120A | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 CA CALCIUM ION × 2 MG MAGNESIUM ION × 4 NA SODIUM ION × 1 ACT ACETATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；17% PEG8000, 200 mM calcium acetate, 100 mM MES/NaOH, pH 6.0
|
Resolution 1.25 Å R-free 0.150 |
| 5WFO Ligand-bound Ras:SOS:Ras complex Deposited 2017-07-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain Q
1–166（166 aa）
Chain R
1–166（166 aa）
|
Not recorded | MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 5UU 6-chloranyl-~{N}-(4-fluorophenyl)-1,2,3,4-tetrahydroacridin-9-amine × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4；293 K；0.1 M sodium acetate, 2.0 M sodium formate, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.99 Å R-free 0.188 |
| 5WFP Ligand-bound Ras:SOS:Ras complex Deposited 2017-07-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain Q
1–166（166 aa）
Chain R
1–166（166 aa）
|
Not recorded | MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 5UX 6-chloranyl-~{N}-(3-chloranyl-4-fluoranyl-phenyl)-1,2,3,4-tetrahydroacridin-9-amine × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4；293 K；0.1 M sodium acetate, 2.0 M sodium formate, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.08 Å R-free 0.203 |
| 5WFQ Ligand-bound Ras:SOS:Ras complex Deposited 2017-07-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain Q
1–166（166 aa）
Chain R
1–166（166 aa）
|
Not recorded | MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 5UV 7-chloranyl-~{N}-(3-chloranyl-4-fluoranyl-phenyl)-1,2,3,4-tetrahydroacridin-9-amine × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4；293 K；0.1 M sodium acetate, 2.0 M sodium formate, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.26 Å R-free 0.196 |
| 5WFR Ligand-bound Ras:SOS:Ras complex Deposited 2017-07-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain Q
1–166（166 aa）
Chain R
1–166（166 aa）
|
Not recorded | MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 5UW ~{N}-(3,3-diphenylpropyl)piperidin-4-amine × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4；293 K；0.1 M sodium acetate, 2.0 M sodium formate, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.46 Å R-free 0.194 |
| 5WPL KRas G12V, bound to GppNHp and miniprotein 225-11 Deposited 2017-08-05 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
1–166（166 aa）
Fragment：residues 1-166
|
Not recorded | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 CA CALCIUM ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；0.1 M ammonium sulfate and 20-25% PEG3,350
|
Resolution 2.15 Å R-free 0.258 |
| 5WPL KRas G12V, bound to GppNHp and miniprotein 225-11 Deposited 2017-08-05 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain D
1–166（166 aa）
Fragment：residues 1-166
|
Not recorded | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；0.1 M ammonium sulfate and 20-25% PEG3,350
|
Resolution 2.15 Å R-free 0.258 |
| 5WPL KRas G12V, bound to GppNHp and miniprotein 225-11 Deposited 2017-08-05 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 3 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain G
1–166（166 aa）
Fragment：residues 1-166
|
Not recorded | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 2 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；0.1 M ammonium sulfate and 20-25% PEG3,350
|
Resolution 2.15 Å R-free 0.258 |
| 5WPL KRas G12V, bound to GppNHp and miniprotein 225-11 Deposited 2017-08-05 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 4 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain J
1–166（166 aa）
Fragment：residues 1-166
|
Not recorded | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 CA CALCIUM ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；0.1 M ammonium sulfate and 20-25% PEG3,350
|
Resolution 2.15 Å R-free 0.258 |
| 5X9S Crystal structure of fully modified H-Ras-GppNHp Deposited 2017-03-09 | Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
Fragment：UNP residues 1-166
|
Not recorded | MG MAGNESIUM ION × 1 CA CALCIUM ION × 3 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；0.1M Ca acetate, 20%(w/v) PEG8000, 0.1M MES (pH6.0)
|
Resolution 2.50 Å R-free 0.236 |
| 621P THREE-DIMENSIONAL STRUCTURES OF H-RAS P21 MUTANTS: MOLECULAR BASIS FOR THEIR INABILITY TO FUNCTION AS SIGNAL SWITCH MOLECULES Deposited 1991-06-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–166（166 aa）
|
Mutation：Q61H | MG MAGNESIUM ION × 2 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.40 Å |
| 6AMB Crystal Structure of the Afadin RA1 domain in complex with HRAS Deposited 2017-08-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–168（168 aa）
|
Not recorded | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 8.9；297 K；PEG3350, K2HPO4
|
Resolution 2.50 Å R-free 0.266 |
| 6AXG Structure of RasGRP4 in complex with HRas Deposited 2017-09-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
1–166（166 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；20% PEG3350 and 300 mM sodium thiocyanate
|
Resolution 3.30 Å R-free 0.260 |
| 6AXG Structure of RasGRP4 in complex with HRas Deposited 2017-09-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain D
1–166（166 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；20% PEG3350 and 300 mM sodium thiocyanate
|
Resolution 3.30 Å R-free 0.260 |
| 6AXG Structure of RasGRP4 in complex with HRas Deposited 2017-09-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain F
1–166（166 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；20% PEG3350 and 300 mM sodium thiocyanate
|
Resolution 3.30 Å R-free 0.260 |
| 6AXG Structure of RasGRP4 in complex with HRas Deposited 2017-09-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain H
1–166（166 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；20% PEG3350 and 300 mM sodium thiocyanate
|
Resolution 3.30 Å R-free 0.260 |
| 6AXG Structure of RasGRP4 in complex with HRas Deposited 2017-09-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 5 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain J
1–166（166 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；20% PEG3350 and 300 mM sodium thiocyanate
|
Resolution 3.30 Å R-free 0.260 |
| 6AXG Structure of RasGRP4 in complex with HRas Deposited 2017-09-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 6 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain L
1–166（166 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；20% PEG3350 and 300 mM sodium thiocyanate
|
Resolution 3.30 Å R-free 0.260 |
| 6BVI Ras:SOS:Ras in complex with a small molecule activator Deposited 2017-12-13 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
1–166（166 aa）
Chain C
1–166（166 aa）
|
Mutation：Y64A Non-standard monomer：Yes (specific site not provided by mmCIF) | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 EC4 6-chloro-N-{1-[(5-chloro-1H-indol-3-yl)methyl]piperidin-4-yl}-L-tryptophanamide × 1 FMT FORMIC ACID × 6 GOL GLYCEROL × 2 NA SODIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 4；292 K；0.1 M sodium acetate, 2.0 M sodium formate, pH 4.0
|
Resolution 1.75 Å R-free 0.169 |
| 6BVJ Ras:SOS:Ras in complex with a small molecule activator Deposited 2017-12-13 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
1–166（166 aa）
Chain C
1–166（166 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 EAS 5-chloro-N-{1-[(5-chloro-1H-indol-3-yl)methyl]piperidin-4-yl}-L-tryptophanamide × 1 FMT FORMIC ACID × 6 GOL GLYCEROL × 2 NA SODIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 4；292 K；0.1 M sodium acetate, 2.0 M sodium formate, pH 4.0
|
Resolution 1.75 Å R-free 0.168 |
| 6BVK Ras:SOS:Ras in complex with a small molecule activator Deposited 2017-12-13 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
1–166（166 aa）
Chain C
1–166（166 aa）
|
Mutation：Y64A Non-standard monomer：Yes (specific site not provided by mmCIF) | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 EAV N-{1-[(5-chloro-1H-indol-3-yl)methyl]piperidin-4-yl}-6-methyl-L-tryptophanamide × 1 FMT FORMIC ACID × 6 GOL GLYCEROL × 2 NA SODIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；292 K；0.1 M sodium acetate, 2.0 M sodium formate, pH 4.0
|
Resolution 1.80 Å R-free 0.179 |
| 6BVL Ras:SOS:Ras in complex with a small molecule activator Deposited 2017-12-13 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
1–166（166 aa）
Chain C
1–166（166 aa）
|
Mutation：Y64A Non-standard monomer：Yes (specific site not provided by mmCIF) | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 EBY N-{1-[(5-chloro-1H-indol-3-yl)methyl]piperidin-4-yl}-5-methyl-L-tryptophanamide × 1 FMT FORMIC ACID × 6 GOL GLYCEROL × 2 NA SODIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4；292 K；0.1 M sodium acetate, 2.0 M sodium formate, pH 4.0
|
Resolution 1.75 Å R-free 0.171 |
| 6BVM Ras:SOS:Ras in complex with a small molecule activator Deposited 2017-12-13 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
1–166（166 aa）
Chain C
1–166（166 aa）
|
Mutation：Y64A Non-standard monomer：Yes (specific site not provided by mmCIF) | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 EBV (2S)-2-amino-1-[(3aR,6aS)-5-[(5-chloro-1H-indol-3-yl)methyl]hexahydropyrrolo[3,4-c]pyrrol-2(1H)-yl]-3-(1H-indol-3-yl)propan-1-one × 1 FMT FORMIC ACID × 6 GOL GLYCEROL × 2 NA SODIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4；292 K；0.1 M sodium acetate, 2.0 M sodium formate, pH 4.0
|
Resolution 1.80 Å R-free 0.178 |
| 6CUO Ras:SOS:Ras in complex with a small molecule activator Deposited 2018-03-26 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
1–166（166 aa）
Chain C
1–166（166 aa）
|
Mutation：Y64A Non-standard monomer：Yes (specific site not provided by mmCIF) | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 FMT FORMIC ACID × 6 FFS N~2~-(3-chlorophenyl)-N~4~-[(furan-2-yl)methyl]quinazoline-2,4-diamine × 1 GOL GLYCEROL × 2 NA SODIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4；293 K；0.1 M sodium acetate, 2.0 M sodium formate, pH 4.0
|
Resolution 1.73 Å R-free 0.178 |
| 6CUP Ras:SOS:Ras in complex with a small molecule activator Deposited 2018-03-26 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
1–166（166 aa）
Chain C
1–166（166 aa）
|
Mutation：Y64A Non-standard monomer：Yes (specific site not provided by mmCIF) | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 FFV N~2~-(3-chloro-4-fluorophenyl)-N~4~-[(1R)-1-cyclopropylethyl]quinazoline-2,4-diamine × 1 FMT FORMIC ACID × 6 GOL GLYCEROL × 2 NA SODIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4；293 K；0.1 M sodium acetate, 2.0 M sodium formate, pH 4.0
|
Resolution 1.83 Å R-free 0.174 |
| 6CUR Ras:SOS:Ras in complex with a small molecule activator Deposited 2018-03-26 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
1–166（166 aa）
Chain C
1–166（166 aa）
|
Mutation：Y64A Non-standard monomer：Yes (specific site not provided by mmCIF) | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 FFY N~2~-(3-chloro-4-fluorophenyl)-N~4~-[(1R)-1-cyclopropylethyl]-8-(1,2,3,6-tetrahydropyridin-4-yl)quinazoline-2,4-diamine × 1 FMT FORMIC ACID × 6 GOL GLYCEROL × 2 NA SODIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4；293 K；0.1 M sodium acetate, 2.0 M sodium formate, pH 4.0
|
Resolution 1.73 Å R-free 0.176 |
| 6D55 Ras:SOS:Ras in complex with a small molecule activator Deposited 2018-04-19 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
1–166（166 aa）
Chain C
1–166（166 aa）
|
Mutation：Y64A Non-standard monomer：Yes (specific site not provided by mmCIF) | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 FWA 6-chloro-2-(2,6-diazaspiro[3.3]heptan-2-yl)-1-[(4-fluoro-3,5-dimethylphenyl)methyl]-4-(4-methylpiperazin-1-yl)-1H-benzimidazole × 1 FMT FORMIC ACID × 6 GOL GLYCEROL × 2 NA SODIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4；293 K；0.1 M sodium acetate, 2.0 M sodium formate, pH 4.0
|
Resolution 1.68 Å R-free 0.178 |
| 6D56 Ras:SOS:Ras in complex with a small molecule activator Deposited 2018-04-19 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
1–166（166 aa）
Chain C
1–166（166 aa）
|
Mutation：Y64A Non-standard monomer：Yes (specific site not provided by mmCIF) | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 FVM 6-chloro-2-(2,6-diazaspiro[3.3]heptan-2-yl)-4-(3,5-dimethyl-1H-pyrazol-4-yl)-1-[(4-fluoro-3,5-dimethylphenyl)methyl]-1H-benzimidazole × 1 FMT FORMIC ACID × 6 GOL GLYCEROL × 2 NA SODIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4；293 K；0.1 M sodium acetate, 2.0 M sodium formate, pH 4.0
|
Resolution 1.68 Å R-free 0.171 |
| 6D59 Ras:SOS:Ras in complex with a small molecule activator Deposited 2018-04-19 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
1–166（166 aa）
Chain C
1–166（166 aa）
|
Mutation：Y64A Non-standard monomer：Yes (specific site not provided by mmCIF) | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 FVJ 6-chloro-4-(3,5-dimethyl-1H-pyrazol-4-yl)-1-[(4-fluoro-3,5-dimethylphenyl)methyl]-2-(piperazin-1-yl)-1H-benzimidazole × 1 FMT FORMIC ACID × 6 GOL GLYCEROL × 2 NA SODIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 1；293 K；0.1 M sodium acetate, 2.0 M sodium formate, pH 4.0
|
Resolution 1.70 Å R-free 0.173 |
| 6D5E Ras:SOS:Ras in complex with a small molecule activator Deposited 2018-04-19 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
1–166（166 aa）
Chain C
1–166（166 aa）
|
Mutation：Y64A Non-standard monomer：Yes (specific site not provided by mmCIF) | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 CL CHLORIDE ION × 4 FVG 1-[(2S)-1-{6-chloro-1-[(4-fluoro-3,5-dimethylphenyl)methyl]-2-(piperazin-1-yl)-1H-benzimidazol-4-yl}pyrrolidin-2-yl]methanamine × 1 FMT FORMIC ACID × 6 GOL GLYCEROL × 3 NA SODIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4；293 K；0.1 M sodium acetate, 2.0 M sodium formate, pH 4.0
|
Resolution 1.75 Å R-free 0.174 |
| 6D5G Ras:SOS:Ras in complex with a small molecule activator Deposited 2018-04-19 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
1–166（166 aa）
Chain C
1–166（166 aa）
|
Mutation：Y64A Non-standard monomer：Yes (specific site not provided by mmCIF) | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 FMT FORMIC ACID × 6 CL CHLORIDE ION × 3 FVD 6-chloro-1-[(4-fluoro-3,5-dimethylphenyl)methyl]-2-(piperazin-1-yl)-4-(1,2,3,6-tetrahydropyridin-4-yl)-1H-benzimidazole × 1 GOL GLYCEROL × 3 BME BETA-MERCAPTOETHANOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；0.1 M sodium acetate, 2.0 M sodium formate, pH 4.0
|
Resolution 1.92 Å R-free 0.172 |
| 6D5H Ras:SOS:Ras in complex with a small molecule activator Deposited 2018-04-19 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
1–166（166 aa）
Chain C
1–166（166 aa）
|
Mutation：Y64A Non-standard monomer：Yes (specific site not provided by mmCIF) | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 CL CHLORIDE ION × 3 FV7 6-chloro-4-(2-chlorophenyl)-1-[(4-fluoro-3,5-dimethylphenyl)methyl]-2-(piperazin-1-yl)-1H-benzimidazole × 1 FMT FORMIC ACID × 5 GOL GLYCEROL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；0.1 M sodium acetate, 2.0 M sodium formate, pH 4.0
|
Resolution 1.80 Å R-free 0.189 |
| 6D5J Ras:SOS:Ras in complex with a small molecule activator Deposited 2018-04-19 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
1–166（166 aa）
Chain C
1–166（166 aa）
|
Mutation：Y64A Non-standard monomer：Yes (specific site not provided by mmCIF) | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 FV4 6-chloro-1-[(4-fluoro-3,5-dimethylphenyl)methyl]-2-(piperazin-1-yl)-1H-benzimidazole × 1 FMT FORMIC ACID × 6 GOL GLYCEROL × 2 NA SODIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；0.1 M sodium acetate, 2.0 M sodium formate, pH 4.0
|
Resolution 1.75 Å R-free 0.175 |
| 6D5L Ras:SOS:Ras in complex with a small molecule activator Deposited 2018-04-19 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
1–166（166 aa）
Chain C
1–166（166 aa）
|
Mutation：Y64A Non-standard monomer：Yes (specific site not provided by mmCIF) | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 FMT FORMIC ACID × 6 FW7 6-chloro-1-[(3-chloro-4-fluorophenyl)methyl]-2-(piperazin-1-yl)-1H-benzimidazole × 1 GOL GLYCEROL × 2 NA SODIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；0.1 M sodium acetate, 2.0 M sodium formate, pH 4.0
|
Resolution 1.70 Å R-free 0.175 |
| 6D5M Ras:SOS:Ras in complex with a small molecule activator Deposited 2018-04-19 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain Q
1–166（166 aa）
Chain R
1–166（166 aa）
|
Mutation：Y64A | MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 FW4 1-[(3-chloro-4-fluorophenyl)methyl]-5,6-dimethyl-2-(piperazin-1-yl)-1H-benzimidazole × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；0.1 M sodium acetate, 2.0 M sodium formate, pH 4.0
|
Resolution 2.08 Å R-free 0.194 |
| 6D5V Ras:SOS:Ras in complex with a small molecule activator Deposited 2018-04-19 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain Q
1–166（166 aa）
Chain R
1–166（166 aa）
|
Mutation：Y64A | MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 FVY 1-[(3-chloro-4-fluorophenyl)methyl]-5,6-dimethyl-1H-benzimidazol-2-amine × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；0.1 M sodium acetate, 2.0 M sodium formate, pH 4.0
|
Resolution 2.04 Å R-free 0.186 |
| 6D5W Ras:SOS:Ras in complex with a small molecule activator Deposited 2018-04-19 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain Q
1–166（166 aa）
Chain R
1–166（166 aa）
|
Mutation：Y64A | MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 FVV 10-[(4-fluorophenyl)methyl]-2,3,4,10-tetrahydropyrimido[1,2-a]benzimidazole × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；0.1 M sodium acetate, 2.0 M sodium formate, pH 4.0
|
Resolution 2.48 Å R-free 0.228 |
| 6DZH HRAS G13D bound to GDP (H13GDP) Deposited 2018-07-04 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
Fragment：residues 1-166
|
Mutation：G13D | GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；291.15 K；5.9% stabilization buffer (20 mM HEPES, 50 mM NaCl, 20 mM MgCl2, pH 7.5)
188.2 mM Calcium Acetate
18.8% PEG 3350
Crystals were grown in 2uL by 2uL drops of mother liquor to protein (7.7mg/mL)
Cryoprotectant for crystal diffraction: 70% mother liquor and 30% glycerol
|
Resolution 1.95 Å R-free 0.218 |
| 6DZH HRAS G13D bound to GDP (H13GDP) Deposited 2018-07-04 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
1–166（166 aa）
Fragment：residues 1-166
|
Mutation：G13D | GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 2 CA CALCIUM ION × 1 DTT 2,3-DIHYDROXY-1,4-DITHIOBUTANE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；291.15 K；5.9% stabilization buffer (20 mM HEPES, 50 mM NaCl, 20 mM MgCl2, pH 7.5)
188.2 mM Calcium Acetate
18.8% PEG 3350
Crystals were grown in 2uL by 2uL drops of mother liquor to protein (7.7mg/mL)
Cryoprotectant for crystal diffraction: 70% mother liquor and 30% glycerol
|
Resolution 1.95 Å R-free 0.218 |
| 6DZH HRAS G13D bound to GDP (H13GDP) Deposited 2018-07-04 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
1–166（166 aa）
Fragment：residues 1-166
|
Mutation：G13D | GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；291.15 K；5.9% stabilization buffer (20 mM HEPES, 50 mM NaCl, 20 mM MgCl2, pH 7.5)
188.2 mM Calcium Acetate
18.8% PEG 3350
Crystals were grown in 2uL by 2uL drops of mother liquor to protein (7.7mg/mL)
Cryoprotectant for crystal diffraction: 70% mother liquor and 30% glycerol
|
Resolution 1.95 Å R-free 0.218 |
| 6E6C HRAS G13D bound to GppNHp (H13GNP) Deposited 2018-07-24 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
Fragment：residues 1-166
|
Not recorded | MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；291.15 K；128 mM Calcium acetate, 20.8% PEG 3350, 20% stabilization buffer (20 mM HEPES, 50 mM NaCl, 20 mM MgCl2, at pH 7.5), Crystals were grown in 2uL by 2 uL drops of mother liquor to protein (22 mg/mL), Cryoprotectant: 70% glycerol and 30% mother liquor
|
Resolution 1.90 Å R-free 0.230 |
| 6E6P HRAS G13D bound to GppNHp (Ha,b,c13GNP) Deposited 2018-07-25 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
Fragment：residues 1-166
|
Mutation：G13D | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 CA CALCIUM ION × 1 GOL GLYCEROL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；291.15 K；152 mM Calcium Acetate, 22.8% PEG 3350, 9.5% stabilization buffer (20 mM HEPES pH 7.5, 50 mM NaCl, 20 mM MgCl2), Crystals were grown in 2uL by 2 uL drops of mother liquor to protein (22 mg/mL), Cryoprotectant: 70% glycerol and 30% mother liquor
|
Resolution 1.93 Å R-free 0.237 |
| 6E6P HRAS G13D bound to GppNHp (Ha,b,c13GNP) Deposited 2018-07-25 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
1–166（166 aa）
Fragment：residues 1-166
|
Mutation：G13D | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；291.15 K；152 mM Calcium Acetate, 22.8% PEG 3350, 9.5% stabilization buffer (20 mM HEPES pH 7.5, 50 mM NaCl, 20 mM MgCl2), Crystals were grown in 2uL by 2 uL drops of mother liquor to protein (22 mg/mL), Cryoprotectant: 70% glycerol and 30% mother liquor
|
Resolution 1.93 Å R-free 0.237 |
| 6E6P HRAS G13D bound to GppNHp (Ha,b,c13GNP) Deposited 2018-07-25 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
1–166（166 aa）
Fragment：residues 1-166
|
Mutation：G13D | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 CA CALCIUM ION × 1 GOL GLYCEROL × 1 NA SODIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；291.15 K；152 mM Calcium Acetate, 22.8% PEG 3350, 9.5% stabilization buffer (20 mM HEPES pH 7.5, 50 mM NaCl, 20 mM MgCl2), Crystals were grown in 2uL by 2 uL drops of mother liquor to protein (22 mg/mL), Cryoprotectant: 70% glycerol and 30% mother liquor
|
Resolution 1.93 Å R-free 0.237 |
| 6MQT HRAS G12S in complex with GDP Deposited 2018-10-10 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
|
Not recorded | GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；293 K；0.2 M Sodium acetate trihydrate, 0.1 M TRIS hydrochloride pH 8.5, 30% w/v Polyethylene glycol 4,000
|
Resolution 1.50 Å R-free 0.195 |
| 6MQT HRAS G12S in complex with GDP Deposited 2018-10-10 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
1–166（166 aa）
|
Not recorded | GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；293 K；0.2 M Sodium acetate trihydrate, 0.1 M TRIS hydrochloride pH 8.5, 30% w/v Polyethylene glycol 4,000
|
Resolution 1.50 Å R-free 0.195 |
| 6MQT HRAS G12S in complex with GDP Deposited 2018-10-10 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
1–166（166 aa）
|
Not recorded | GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；293 K；0.2 M Sodium acetate trihydrate, 0.1 M TRIS hydrochloride pH 8.5, 30% w/v Polyethylene glycol 4,000
|
Resolution 1.50 Å R-free 0.195 |
| 6MQT HRAS G12S in complex with GDP Deposited 2018-10-10 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
1–166（166 aa）
|
Not recorded | GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；293 K；0.2 M Sodium acetate trihydrate, 0.1 M TRIS hydrochloride pH 8.5, 30% w/v Polyethylene glycol 4,000
|
Resolution 1.50 Å R-free 0.195 |
| 6MQT HRAS G12S in complex with GDP Deposited 2018-10-10 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 5 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain E
1–166（166 aa）
|
Not recorded | GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；293 K；0.2 M Sodium acetate trihydrate, 0.1 M TRIS hydrochloride pH 8.5, 30% w/v Polyethylene glycol 4,000
|
Resolution 1.50 Å R-free 0.195 |
| 6MQT HRAS G12S in complex with GDP Deposited 2018-10-10 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 6 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain F
1–166（166 aa）
|
Not recorded | GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；293 K；0.2 M Sodium acetate trihydrate, 0.1 M TRIS hydrochloride pH 8.5, 30% w/v Polyethylene glycol 4,000
|
Resolution 1.50 Å R-free 0.195 |
| 6MQT HRAS G12S in complex with GDP Deposited 2018-10-10 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 7 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain G
1–166（166 aa）
|
Not recorded | GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；293 K；0.2 M Sodium acetate trihydrate, 0.1 M TRIS hydrochloride pH 8.5, 30% w/v Polyethylene glycol 4,000
|
Resolution 1.50 Å R-free 0.195 |
| 6MQT HRAS G12S in complex with GDP Deposited 2018-10-10 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 8 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain H
1–166（166 aa）
|
Not recorded | GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；293 K；0.2 M Sodium acetate trihydrate, 0.1 M TRIS hydrochloride pH 8.5, 30% w/v Polyethylene glycol 4,000
|
Resolution 1.50 Å R-free 0.195 |
| 6NTC Crystal Structure of G12V HRas-GppNHp bound in complex with the engineered RBD variant 1 of CRAF Kinase protein Deposited 2019-01-28 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–166（166 aa）
|
Mutation：G12V | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 GOL GLYCEROL × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；293.15 K；30% PEG4000, 200mM NH4SO4, 100mM Na CaCo pH 6.5
|
Resolution 2.90 Å R-free 0.278 |
| 6NTD Crystal Structure of G12V HRas-GppNHp bound in complex with the engineered RBD variant 12 of CRAF Kinase protein Deposited 2019-01-28 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–166（166 aa）
|
Mutation：G12V | MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.6；293.15 K；30% PEG4000, 200mM NH4SO4, 100mM Na Citrate
|
Resolution 3.15 Å R-free 0.287 |
| 6Q21 MOLECULAR SWITCH FOR SIGNAL TRANSDUCTION: STRUCTURAL DIFFERENCES BETWEEN ACTIVE AND INACTIVE FORMS OF PROTOONCOGENIC RAS PROTEINS Deposited 1992-07-27 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–171（171 aa）
Chain B
1–171（171 aa）
Chain C
1–171（171 aa）
Chain D
1–171（171 aa）
|
Not recorded | MG MAGNESIUM ION × 4 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 4 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.95 Å |
| 6V94 Expanding the Chemical Landscape of SOS1 Activators Using Fragment Based Methods Deposited 2019-12-13 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
1–166（166 aa）
Chain C
1–166（166 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 QTV 1-[(4-fluorophenyl)methyl]-2-methyl-4-nitro-1H-imidazole × 1 FMT FORMIC ACID × 6 GOL GLYCEROL × 2 NA SODIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
EVAPORATION；pH 4；293 K；0.1 M sodium acetate, 2.0 M sodium formate, pH 4.0
|
Resolution 1.80 Å R-free 0.172 |
| 6V9F Expanding the Chemical Landscape of SOS1 Activators Using Fragment Based Methods Deposited 2019-12-13 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
1–166（166 aa）
Chain C
1–166（166 aa）
|
Mutation：Y64A Non-standard monomer：Yes (specific site not provided by mmCIF) | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 FMT FORMIC ACID × 6 QTS 1-[(4-chlorophenyl)methyl]-1H-benzimidazol-2-amine × 1 GOL GLYCEROL × 2 NA SODIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
EVAPORATION；pH 4；293 K；0.1 M sodium acetate, 2.0 M sodium formate, pH 4.0
|
Resolution 1.85 Å R-free 0.171 |
| 6V9J Expanding the Chemical Landscape of SOS1 Activators Using Fragment Based Methods Deposited 2019-12-13 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
1–166（166 aa）
Chain C
1–166（166 aa）
|
Mutation：Y64A Non-standard monomer：Yes (specific site not provided by mmCIF) | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 QTM 3-(2-aminoethyl)-4-(3-chloro-4-fluorophenoxy)benzene-1-sulfonamide × 1 FMT FORMIC ACID × 6 GOL GLYCEROL × 2 NA SODIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4；293 K；0.1 M sodium acetate, 2.0 M sodium formate, pH 4.0
|
Resolution 1.76 Å R-free 0.166 |
| 6V9L Expanding the Chemical Landscape of SOS1 Activators Using Fragment Based Methods Deposited 2019-12-13 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
1–166（166 aa）
Chain C
1–166（166 aa）
|
Mutation：Y64A Non-standard monomer：Yes (specific site not provided by mmCIF) | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ACT ACETATE ION × 1 QTJ 4-(3-chloro-4-fluorophenoxy)benzene-1-sulfonamide × 1 FMT FORMIC ACID × 6 GOL GLYCEROL × 2 NA SODIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4；293 K；0.1 M sodium acetate, 2.0 M sodium formate, pH 4.0
|
Resolution 1.70 Å R-free 0.175 |
| 6V9M Expanding the Chemical Landscape of SOS1 Activators Using Fragment Based Methods Deposited 2019-12-13 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
1–166（166 aa）
Chain C
1–166（166 aa）
|
Mutation：Y64A Non-standard monomer：Yes (specific site not provided by mmCIF) | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 QTG 4-fluoro-2-methyl-N-propylbenzene-1-sulfonamide × 1 FMT FORMIC ACID × 6 GOL GLYCEROL × 2 NA SODIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；0.1 M sodium acetate, 2.0 M sodium formate, pH 4.0
|
Resolution 1.65 Å R-free 0.180 |
| 6V9N Expanding the Chemical Landscape of SOS1 Activators Using Fragment Based Methods Deposited 2019-12-13 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
1–166（166 aa）
Chain C
1–166（166 aa）
|
Mutation：Y64A Non-standard monomer：Yes (specific site not provided by mmCIF) | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 FMT FORMIC ACID × 6 QTD 4-phenoxybenzene-1-sulfonamide × 1 GOL GLYCEROL × 2 NA SODIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；0.1 M sodium acetate, 2.0 M sodium formate, pH 4.0
|
Resolution 1.65 Å R-free 0.177 |
| 6V9O Expanding the Chemical Landscape of SOS1 Activators Using Fragment Based Methods Deposited 2019-12-13 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
1–166（166 aa）
Chain C
1–166（166 aa）
|
Mutation：Y64A Non-standard monomer：Yes (specific site not provided by mmCIF) | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 QTA 3-(phenylsulfonyl)benzene-1-sulfonamide × 2 FMT FORMIC ACID × 7 GOL GLYCEROL × 2 NA SODIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；0.1 M sodium acetate, 2.0 M sodium formate, pH 4.0
|
Resolution 1.80 Å R-free 0.169 |
| 6ZJ0 CRYSTAL STRUCTURE OF HRAS-G12D IN COMPLEX WITH GCP AND COMPOUND 18 Deposited 2020-06-26 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
|
Not recorded | MG MAGNESIUM ION × 2 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 EZZ (3~{S})-3-[2-[(dimethylamino)methyl]-1~{H}-indol-3-yl]-5-oxidanyl-2,3-dihydroisoindol-1-one × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；278 K；30% PEG400, 200mM MgCl2, 100mM TRIS PH=8,5
|
Resolution 1.76 Å R-free 0.228 |
| 6ZL3 CRYSTAL STRUCTURE OF HRAS IN COMPLEX WITH COMPOUND 18 and GDP Deposited 2020-06-30 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
|
Not recorded | NA SODIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 EZZ (3~{S})-3-[2-[(dimethylamino)methyl]-1~{H}-indol-3-yl]-5-oxidanyl-2,3-dihydroisoindol-1-one × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；278 K；160mM Calcium acetate
20% Glycerol
14.4% PEG8000
80mM Sodium cacodylate
|
Resolution 2.03 Å R-free 0.223 |
| 721P THREE-DIMENSIONAL STRUCTURES OF H-RAS P21 MUTANTS: MOLECULAR BASIS FOR THEIR INABILITY TO FUNCTION AS SIGNAL SWITCH MOLECULES Deposited 1991-06-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–166（166 aa）
|
Mutation：Q61L | MG MAGNESIUM ION × 2 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.00 Å |
| 7DPH H-Ras Q61H in complex with GppNHp (state 1) after structural transition by humidity control Deposited 2020-12-19 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
1–166（166 aa）
|
Mutation：Q61H | MG MAGNESIUM ION × 6 NA SODIUM ION × 3 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5；293 K；PEG400, SODIUM ACETATE, CALCIUM ACETATE
|
Resolution 1.54 Å R-free 0.202 |
| 7DPJ H-Ras Q61L in complex with GppNHp (state 1) after structural transition by humidity control Deposited 2020-12-19 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
|
Mutation：Q61L | MG MAGNESIUM ION × 2 CA CALCIUM ION × 3 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；PEG400, SODIUM ACETATE, CALCIUM
ACETATE
|
Resolution 1.98 Å R-free 0.221 |
| 7JHP Crystal structure of HRas in complex with the Ras-binding and cysteine-rich domains of CRaf-kinase Deposited 2020-07-21 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–166（166 aa）
Fragment：UNP residues 1-166
|
Mutation：R97C | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ZN ZINC ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；291 K；0.1 M ammonium acetate, 0.1 M Bis-Tris, pH 5.5, 17% w/v PEG10000
|
Resolution 2.77 Å R-free 0.264 |
| 7JIF HRAS A59T GppNHp Deposited 2020-07-23 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
|
Mutation：A59T | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；291.15 K；Starting concentration of HRAS A59T GppNHp was 12.1mg/mL in buffer containing 20mM HEPES, 50mM NaCl, 20mM MgCl2 and 1mM DTT at pH 7.5.
Used a 24-well plate sealed with Vaseline and total well volumes of 0.402mL. Hanging drops were 0.002mL mother liquor to 0.002mL protein. Mother liquor contained 2.6mM NaCl, 1mM MgCl2, 15.7mM HEPES (pH7.5), 2.5mM DTT, 37.3mM Ca(OAc)2, and 20.5% PEG 3350
|
Resolution 1.76 Å R-free 0.250 |
| 7JIG HRAS A59T GppNHp crystal 2 Deposited 2020-07-23 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
|
Mutation：A59T | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；291.15 K；Starting concentration of HRAS A59T GppNHp was 12.1mg/mL in buffer containing 20mM HEPES, 50mM NaCl, 20mM MgCl2 and 1mM DTT at pH 7.5.
Used a 24-well plate sealed with Vaseline and total well volumes of 0.402mL. Hanging drops were 0.001mL mother liquor to 0.001mL protein. Mother liquor contained 3.4mM NaCl, 1mM MgCl2, 15.7mM HEPES (pH7.5), 2.5mM DTT, 10mM Mg(OAc)2, and 44.8% PEG 400.
|
Resolution 2.32 Å R-free 0.264 |
| 7JIH HRAS A59E GppNHp Deposited 2020-07-23 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
|
Mutation：A59E | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；291.15 K；Starting concentration of HRAS A59T GppNHp was 18.1mg/mL in buffer containing 20mM HEPES, 50mM NaCl, 20mM MgCl2 and 1mM DTT at pH 7.5.
Used a 24-well plate sealed with Vaseline and total well volumes of 0.402mL. Hanging drops were 0.001mL mother liquor to 0.001mL protein. Mother liquor contained 2.6mM NaCl, 1mM MgCl2, 15.7mM HEPES (pH7.5), 2.5mM DTT, 9.95mM Ca(OAc)2, and 19.9% PEG 3350.
|
Resolution 1.99 Å R-free 0.254 |
| 7JIH HRAS A59E GppNHp Deposited 2020-07-23 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
1–166（166 aa）
|
Mutation：A59E | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；291.15 K；Starting concentration of HRAS A59T GppNHp was 18.1mg/mL in buffer containing 20mM HEPES, 50mM NaCl, 20mM MgCl2 and 1mM DTT at pH 7.5.
Used a 24-well plate sealed with Vaseline and total well volumes of 0.402mL. Hanging drops were 0.001mL mother liquor to 0.001mL protein. Mother liquor contained 2.6mM NaCl, 1mM MgCl2, 15.7mM HEPES (pH7.5), 2.5mM DTT, 9.95mM Ca(OAc)2, and 19.9% PEG 3350.
|
Resolution 1.99 Å R-free 0.254 |
| 7JII HRAS A59E GDP Deposited 2020-07-23 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
|
Mutation：A59E | GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 CA CALCIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；291.15 K；Starting concentration of HRAS A59T GppNHp was 15.2mg/mL in buffer containing 20mM HEPES, 50mM NaCl, 20mM MgCl2 and 1mM DTT at pH 7.5.
Used a 24-well plate sealed with Vaseline and total well volumes of 0.402mL. Hanging drops were 0.001mL mother liquor to 0.001mL protein. Mother liquor contained 2.6mM NaCl, 1mM MgCl2, 15.7mM HEPES (pH7.5), 2.5mM DTT, 43.5mM Ca(OAc)2, and 20.5% PEG 3350.
|
Resolution 1.53 Å R-free 0.203 |
| 7JII HRAS A59E GDP Deposited 2020-07-23 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
1–166（166 aa）
|
Mutation：A59E | GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 CA CALCIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；291.15 K；Starting concentration of HRAS A59T GppNHp was 15.2mg/mL in buffer containing 20mM HEPES, 50mM NaCl, 20mM MgCl2 and 1mM DTT at pH 7.5.
Used a 24-well plate sealed with Vaseline and total well volumes of 0.402mL. Hanging drops were 0.001mL mother liquor to 0.001mL protein. Mother liquor contained 2.6mM NaCl, 1mM MgCl2, 15.7mM HEPES (pH7.5), 2.5mM DTT, 43.5mM Ca(OAc)2, and 20.5% PEG 3350.
|
Resolution 1.53 Å R-free 0.203 |
| 7L0F Monobody 12VC3 Bound to HRAS(WT) Deposited 2020-12-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain E
1–166（166 aa）
|
Not recorded | MG MAGNESIUM ION × 1 GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；291 K；0.225 M Potassium sodium tartrate tetrahydrate, 15% w/v Polyethylene glycol 3,350
|
Resolution 1.98 Å R-free 0.191 |
| 7L0F Monobody 12VC3 Bound to HRAS(WT) Deposited 2020-12-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–166（166 aa）
|
Not recorded | MG MAGNESIUM ION × 1 GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；291 K；0.225 M Potassium sodium tartrate tetrahydrate, 15% w/v Polyethylene glycol 3,350
|
Resolution 1.98 Å R-free 0.191 |
| 7L0F Monobody 12VC3 Bound to HRAS(WT) Deposited 2020-12-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain G
1–166（166 aa）
|
Not recorded | MG MAGNESIUM ION × 1 GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；291 K；0.225 M Potassium sodium tartrate tetrahydrate, 15% w/v Polyethylene glycol 3,350
|
Resolution 1.98 Å R-free 0.191 |
| 7L0F Monobody 12VC3 Bound to HRAS(WT) Deposited 2020-12-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain L
1–166（166 aa）
|
Not recorded | MG MAGNESIUM ION × 1 GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；291 K；0.225 M Potassium sodium tartrate tetrahydrate, 15% w/v Polyethylene glycol 3,350
|
Resolution 1.98 Å R-free 0.191 |
| 7L0G Monobody 12VC1 Bound to HRAS(G12C) Deposited 2020-12-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–166（166 aa）
|
Mutation：G12C | GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；291 K；0.225 M Potassium sodium tartrate tetrahydrate, 15% w/v Polyethylene glycol 3,350
|
Resolution 2.54 Å R-free 0.229 |
| 7L0G Monobody 12VC1 Bound to HRAS(G12C) Deposited 2020-12-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
1–166（166 aa）
|
Mutation：G12C | GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；291 K；0.225 M Potassium sodium tartrate tetrahydrate, 15% w/v Polyethylene glycol 3,350
|
Resolution 2.54 Å R-free 0.229 |
| 7L0G Monobody 12VC1 Bound to HRAS(G12C) Deposited 2020-12-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain E
1–166（166 aa）
|
Mutation：G12C | GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；291 K；0.225 M Potassium sodium tartrate tetrahydrate, 15% w/v Polyethylene glycol 3,350
|
Resolution 2.54 Å R-free 0.229 |
| 7L0G Monobody 12VC1 Bound to HRAS(G12C) Deposited 2020-12-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain G
1–166（166 aa）
|
Mutation：G12C | GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；291 K；0.225 M Potassium sodium tartrate tetrahydrate, 15% w/v Polyethylene glycol 3,350
|
Resolution 2.54 Å R-free 0.229 |
| 7OG9 GTPase HRAS under ambient pressure Deposited 2021-05-06 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
Fragment：GTPase HRAS N-terminally processed
|
Not recorded | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 PGE TRIETHYLENE GLYCOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；pH 7.5；298 K；40 mM Tris HCL, 10 mM MgCl2, 2 mM DTE, 26-30 % PEG-400
|
Resolution 1.75 Å R-free 0.217 |
| 7OGA GTPase HRAS under 200 MPa pressure Deposited 2021-05-06 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
Fragment：GTPase HRAS N-terminally processed
|
Not recorded | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 PGE TRIETHYLENE GLYCOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；pH 7.5；298 K；40 mM Tris HCL, 10 mM MgCl2, 2 mM DTE, 26-30 % PEG-400
|
Resolution 1.90 Å R-free 0.235 |
| 7OGB GTPase HRAS under 500 MPa pressure Deposited 2021-05-06 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
Fragment：GTPase HRAS N-terminally processed
|
Not recorded | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 PGE TRIETHYLENE GLYCOL × 2 1PE PENTAETHYLENE GLYCOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；pH 7.5；298 K；40 mM Tris HCL, 10 mM MgCl2, 2 mM DTE, 26-30 % PEG-400
|
Resolution 1.85 Å R-free 0.236 |
| 7OGC GTPase HRAS under 650 MPa pressure Deposited 2021-05-06 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
Fragment：GTPase HRAS N-terminally processed
|
Not recorded | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 PGE TRIETHYLENE GLYCOL × 2 1PE PENTAETHYLENE GLYCOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；pH 7.5；298 K；40 mM Tris HCL, 10 mM MgCl2, 2 mM DTE, 26-30 % PEG-400
|
Resolution 1.70 Å R-free 0.221 |
| 7OGD GTPase HRAS mutant D33K under ambient pressure Deposited 2021-05-06 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
Fragment：GTPase HRAS N-terminally processed
|
Mutation：D33K | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 PGE TRIETHYLENE GLYCOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；pH 7.5；298 K；40 mM Tris HCL, 10 mM MgCl2, 2 mM DTE, 26-30 % PEG-400
|
Resolution 1.95 Å R-free 0.250 |
| 7OGE GTPase HRAS mutant D33K under 200 MPa pressure Deposited 2021-05-06 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
Fragment：GTPase HRAS N-terminally processed
|
Mutation：D33K | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 PGE TRIETHYLENE GLYCOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；pH 7.5；298 K；40 mM Tris HCL, 10 mM MgCl2, 2 mM DTE, 26-30 % PEG-400
|
Resolution 2.10 Å R-free 0.263 |
| 7OGF GTPase HRAS mutant D33K under 900 MPa pressure Deposited 2021-05-06 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
Fragment：GTPase HRAS N-terminally processed
|
Mutation：D33K | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 PGE TRIETHYLENE GLYCOL × 2 1PE PENTAETHYLENE GLYCOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；pH 7.5；298 K；40 mM Tris HCL, 10 mM MgCl2, 2 mM DTE, 26-30 % PEG-400
|
Resolution 1.80 Å R-free 0.333 |
| 7TAM HRas G12V in complex with GDP Deposited 2021-12-21 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
|
Mutation：G12V | GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 2 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；295.15 K；29% PEG 3350, 0.2 M calcium chloride, 0.1 M TRIS hydrochloride
|
Resolution 1.87 Å R-free 0.201 |
| 7VV8 Crystal Structure of HRasQ61L(GMPPNP-bound) in complex with the Ras-binding domain(RBD) of SIN1 Deposited 2021-11-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–166（166 aa）
|
Not recorded | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；277 K；PEG20000, 0.1M Sodim citrate. pH 5.0
|
Resolution 1.70 Å R-free 0.199 |
| 7VV9 Crystal Structure of HRas(GMPPNP-bound) in complex with the Ras-binding domain(RBD) of SIN1 Deposited 2021-11-05 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–170（170 aa）
|
Not recorded | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5；278 K；PEG 20000, Sodium citrate
|
Resolution 1.60 Å R-free 0.200 |
| 7VVG Crystal Structure of HRasG12V(GMPPNP-bound) in complex with the Ras-binding domain(RBD) of SIN1 Deposited 2021-11-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–166（166 aa）
|
Not recorded | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5；278 K；PEG2000
|
Resolution 1.70 Å R-free 0.202 |
| 821P THREE-DIMENSIONAL STRUCTURES AND PROPERTIES OF A TRANSFORMING AND A NONTRANSFORMING GLYCINE-12 MUTANT OF P21H-RAS Deposited 1993-03-29 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–166（166 aa）
|
Mutation：G12P | MG MAGNESIUM ION × 2 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.50 Å |
| 8BE6 Crystal structure of SOS1-HRas-peptidomimetic2 Deposited 2022-10-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain R
1–166（166 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；277 K；sodium formate 3 M, Tris 100mM pH 8.0
|
Resolution 2.90 Å R-free 0.273 |
| 8BE7 Crystal structure of SOS1-HRas-peptidomimetic3 Deposited 2022-10-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain R
1–166（166 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；277 K；sodium formate 3 M, Tris 100mM pH 8.0
|
Resolution 3.00 Å R-free 0.267 |
| 8BE8 Crystal structure of SOS1-HRas-peptidomimetic4 Deposited 2022-10-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain R
1–166（166 aa）
|
Not recorded | FMT FORMIC ACID × 8 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；277 K；sodium formate 3 M, Tris 100mM pH 8.0
|
Resolution 2.40 Å R-free 0.237 |
| 8BE9 Crystal structure of SOS1-HRas-peptidomimetic5 Deposited 2022-10-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain R
1–166（166 aa）
|
Not recorded | CL CHLORIDE ION × 3 FMT FORMIC ACID × 14 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；277 K；sodium formate 3 M, Tris 100mM pH 8.0
|
Resolution 2.51 Å R-free 0.229 |
| 8BEA Crystal structure of SOS1-HRas-peptidomimetic10 Deposited 2022-10-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain R
1–166（166 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；277 K；sodium formate 3 M, Tris 100mM pH 8.0
|
Resolution 2.47 Å R-free 0.233 |
| 8BOS Transition state analogue complex of small G protein and its GAP effector Deposited 2022-11-15 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain R
1–166（166 aa）
Fragment：GTPase HRAS N-terminally processed
|
Not recorded | GDP GUANOSINE-5'-DIPHOSPHATE × 1 MGF TRIFLUOROMAGNESATE × 1 MG MAGNESIUM ION × 1 GAI GUANIDINE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；precipitant: HEPES-Na 100 mM pH = 8.0, PEG3350 22% (w/v), (NH4)2SO4 20 mM, Gd-HCl 100 mM, NaF 20 mM
protein buffer: HRas 0.400 mM, RasGAP 0.400 mM, HEPES-Na 20 mM, NaF 20 mM
drop size: 5 uL, protein:precipitant ratio: 1:1.2
|
Resolution 2.10 Å R-free 0.280 |
| 8BWG HRas (1-166) Y64 phosphorylation Deposited 2022-12-06 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain R
1–166（166 aa）
Fragment：GTPase HRAS N-terminally processed
|
Not recorded | GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；Using a commercial crystal screen (HAMPTON RESEARCH, HR2-130) yielded a hit for monophosphorylated HRas under sitting drop conditions (drop size 600 nL) with a 1:1 ratio of protein solution (phospho-HRas 0.4 mM, RasGAP 0.4 mM, Na-HEPES 20 mM pH = 8.0, MgCl2 5 mM, NaF 20 mM) and precipitant (Na-citrate 100 mM pH = 5.6, Li2SO4 1.0 M, CaCl2 200 mM). After three rounds of microseeding well-formed single crystals were obtained using 2.0 uL sitting drops and a 1:1 ratio of protein buffer (HRas 400 uM, RasGAP 400 uM, MgCl2 5 mM, Na-HEPES 20 mM pH = 8.0, NaF 20 mM) and precipitant (Na-Citrate 100 mM pH = 5.6, Li2SO4 800 mM, CaCl2 200 mM). These were harvested using cryoprotectant (80% precipitant, 20% glycerol (v/v)) and sent for data collection.
|
Resolution 1.32 Å R-free 0.174 |
| 8CNJ HRas(1-166) in complex with GDP and BeF3- Deposited 2023-02-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–166（166 aa）
Fragment：GTPase HRAS N-terminally processed
Chain B
1–166（166 aa）
Fragment：GTPase HRAS N-terminally processed
|
Not recorded | GDP GUANOSINE-5'-DIPHOSPHATE × 2 BEF BERYLLIUM TRIFLUORIDE ION × 2 MG MAGNESIUM ION × 2 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；BeF3- GSA complexes were obtained under vapour diffusion sitting drop conditions. Protein buffer (HRas pY64 or HRas 0.4 mM, Na-HEPES 20 mM pH = 8.0, MgCl2 5 mM, NaF 20 mM) were mixed with precipitant in a 1:1 ratio with a total drop size of 600 nL. The precipitant solution consited of: (30 % (v/v) MPD, 100 mM imidazole, pH = 7.0). Protein crystals were soaked in their respective precipitant solutions containing 50 mM BeCl2 and subsequently flash-frozen using 20% glycerol as cryoprotectant.
|
Resolution 1.35 Å R-free 0.179 |
| 8CNN BeF3 Phospho-HRas GSA complex Deposited 2023-02-23 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | PEG DI(HYDROXYETHYL)ETHER × 2 ACT ACETATE ION × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 1 BEF BERYLLIUM TRIFLUORIDE ION × 1 MG MAGNESIUM ION × 1 SO4 SULFATE ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；Protein buffer (phospho-HRas pY64 0.4 mM, RasGAP 0.4 mM, Na-HEPES 20 mM pH = 8.0, MgCl2 5 mM, NaF 20 mM) was mixed with precipitant in a 1:1 ratio with a total drop size of 600 nL. The precipitant solution consits of: 100 mM NaOAc, pH = 4.5, 200 mM Li2SO4, 50% PEG400 (v/v). Protein crystals were soaked in precipitant solutions containing 50-100 mM BeCl2 and subsequently flash-frozen using 20% glycerol as cryoprotectant.
|
Resolution 1.48 Å R-free 0.188 |
| 8ELK HRAS R97F Crystal Form 1 Deposited 2022-09-26 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–189（189 aa）
|
Mutation：R97F | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 2 CA CALCIUM ION × 2 NA SODIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；291.15 K；PEG 3350,
Calcium Acetate,
Magnesium Chloride,
Sodium Chloride,
HEPES
|
Resolution 1.80 Å R-free 0.195 |
| 8ELR HRAS R97F Crystal Form 2 Deposited 2022-09-26 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–189（189 aa）
|
Mutation：R97F | MG MAGNESIUM ION × 2 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；291.15 K；Calcium Acetate
PEG3350,
Magnesium Chloride,
HEPES,
Sodium Chloride,
DTT
|
Resolution 2.05 Å R-free 0.244 |
| 8ELS HRAS R97A Crystal Form 1 R-state Deposited 2022-09-26 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–189（189 aa）
|
Mutation：R97A | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 CA CALCIUM ION × 2 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；277.15 K；PEG 3350,
Calcium Acetate,
HEPES,
Calcium Chloride,
Magnesium Chloride
|
Resolution 2.27 Å R-free 0.211 |
| 8ELT HRAS R97G Crystal Form 2 Deposited 2022-09-26 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–189（189 aa）
|
Mutation：R97G | MG MAGNESIUM ION × 2 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 CA CALCIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；291.15 K；PEG 3350,
Calcium Acetate,
Magnesium Chloride,
Sodium Chloride,
DTT
|
Resolution 1.66 Å R-free 0.239 |
| 8ELU HRAS R97G Crystal form 1 Deposited 2022-09-26 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–189（189 aa）
|
Mutation：R97G | MG MAGNESIUM ION × 2 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；291.15 K；PEG 3350,
Calcium Acetate,
Magnesium Chloride,
Sodium Chloride,
DTT
|
Resolution 1.93 Å R-free 0.209 |
| 8ELV HRAS R97I Crystal Form 2 Deposited 2022-09-26 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–189（189 aa）
|
Mutation：R97I | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 CA CALCIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；291.15 K；PEG 3350,
Calcium Acetate,
Magnesium Chloride,
Sodium Chloride,
DTT
|
Resolution 2.15 Å R-free 0.270 |
| 8ELW HRAS R97A Crystal Form 1 T-State Deposited 2022-09-26 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–189（189 aa）
|
Mutation：R97A | CA CALCIUM ION × 2 MG MAGNESIUM ION × 2 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；291.15 K；PEG 3350,
Calcium Acetate,
Sodium Chloride,
Magnesium Chloride,
HEPES
|
Resolution 1.70 Å R-free 0.186 |
| 8ELX HRAS R97I Crystal Form 1 Deposited 2022-09-26 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–189（189 aa）
|
Mutation：R97I | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 CA CALCIUM ION × 1 MG MAGNESIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；291.15 K；PEG 3350,
Calcium Acetate,
Magnesium Chloride,
Sodium Chloride,
DTT
|
Resolution 1.98 Å R-free 0.286 |
| 8ELY HRAS R97M Crystal Form 2 Deposited 2022-09-26 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–189（189 aa）
|
Mutation：R97M | MG MAGNESIUM ION × 2 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 CA CALCIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；291.15 K；PEG 3350,
Calcium Acetate,
Magnesium Chloride,
Sodium Chloride,
DTT
|
Resolution 1.75 Å R-free 0.227 |
| 8ELZ HRAS R97M Crystal Form 1 Deposited 2022-09-26 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–189（189 aa）
|
Mutation：R97M | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 2 DTT 2,3-DIHYDROXY-1,4-DITHIOBUTANE × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；291.15 K；PEG 3350,
Calcium Acetate,
Magnesium Chloride,
Sodium Chloride,
DTT
|
Resolution 1.96 Å R-free 0.202 |
| 8EM0 HRAS R97V Crystal Form 1 Deposited 2022-09-26 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–189（189 aa）
|
Mutation：R97V | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 CA CALCIUM ION × 1 MG MAGNESIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；291.15 K；PEG 3350,
Calcium Acetate,
Magnesium Chloride,
Sodium Chloride,
DTT
|
Resolution 2.11 Å R-free 0.201 |
| 8FG3 HRAS R97L Crystal Form 2 Deposited 2022-12-12 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
|
Mutation：R97L | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；291.15 K；PEG 3350,
Calcium Acetate,
Magnesium Chloride,
Sodium Chloride,
DTT
|
Resolution 1.49 Å R-free 0.247 |
| 8FG4 HRAS R97L Crystal Form 1 Deposited 2022-12-12 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
|
Mutation：R97L | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 CA CALCIUM ION × 1 MG MAGNESIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；291.15 K；PEG 3350,
Calcium Acetate,
Magnesium Chloride,
Sodium Chloride,
DTT
|
Resolution 1.85 Å R-free 0.227 |
| 8OSM GTPASE HRAS IN COMPLEX WITH ZN-CYCLEN AT 200 MPA PRESSURE Deposited 2023-04-19 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
Fragment：GTPase HRAS N-terminally processed
|
Not recorded | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 PGE TRIETHYLENE GLYCOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；pH 7.5；293 K；40 MM TRIS HCL, 10 MM MGCL2, 2 MM DTE,
26-30 % PEG-400
|
Resolution 2.05 Å R-free 0.224 |
| 8OSN GTPASE HRAS IN COMPLEX WITH ZN-CYCLEN AT AMBIENT PRESSURE Deposited 2023-04-19 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
Fragment：GTPase HRAS N-terminally processed
|
Not recorded | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 PGE TRIETHYLENE GLYCOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；pH 7.5；293 K；40 MM TRIS HCL, 10 MM MGCL2, 2 MM DTE, 26-30 % PEG-400
|
Resolution 1.80 Å R-free 0.215 |
| 8OSO GTPase HRAS in complex with Zn-cyclen under 500 MPa pressure Deposited 2023-04-19 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
Fragment：GTPase HRAS N-terminally processed
|
Not recorded | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 PGE TRIETHYLENE GLYCOL × 1 YCN 1,4,7,10-tetraazacyclododecane × 1 ZN ZINC ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；pH 7.5；293 K；40 MM TRIS HCL, 10 MM MGCL2, 2 MM DTE,
26-30 % PEG-400
|
Resolution 2.50 Å R-free 0.280 |
| 8TBG Tricomplex of RMC-7977, HRAS WT, and CypA Deposited 2023-06-28 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–166（166 aa）
|
Not recorded | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ZNI (1R,5S,6r)-N-[(1P,7S,9S,13S,20M)-20-{5-(4-cyclopropylpiperazin-1-yl)-2-[(1S)-1-methoxyethyl]pyridin-3-yl}-21-ethyl-17,17-dimethyl-8,14-dioxo-15-oxa-4-thia-9,21,27,28-tetraazapentacyclo[17.5.2.1~2,5~.1~9,13~.0~22,26~]octacosa-1(24),2,5(28),19,22,25-hexaen-7-yl]-3-oxabicyclo[3.1.0]hexane-6-carboxamide × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；291 K；28% PEG4000, 0.1 M Tris, pH 8
|
Resolution 1.20 Å R-free 0.192 |
| 8TBG Tricomplex of RMC-7977, HRAS WT, and CypA Deposited 2023-06-28 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
1–166（166 aa）
|
Not recorded | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 GOL GLYCEROL × 1 ZNI (1R,5S,6r)-N-[(1P,7S,9S,13S,20M)-20-{5-(4-cyclopropylpiperazin-1-yl)-2-[(1S)-1-methoxyethyl]pyridin-3-yl}-21-ethyl-17,17-dimethyl-8,14-dioxo-15-oxa-4-thia-9,21,27,28-tetraazapentacyclo[17.5.2.1~2,5~.1~9,13~.0~22,26~]octacosa-1(24),2,5(28),19,22,25-hexaen-7-yl]-3-oxabicyclo[3.1.0]hexane-6-carboxamide × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；291 K；28% PEG4000, 0.1 M Tris, pH 8
|
Resolution 1.20 Å R-free 0.192 |
| 8TLR Crystal Structure of human HRAS G12C covalently bound to AMG 510 Deposited 2023-07-27 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
|
Mutation：G12C | GDP GUANOSINE-5'-DIPHOSPHATE × 1 MOV AMG 510 (bound form) × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；0.1 M MES
30% PEG 4000
|
Resolution 1.70 Å R-free 0.210 |
| 8WWC De novo design binder of HRAS -120-4 Deposited 2023-10-25 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–166（166 aa）
|
Not recorded | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；289 K；0.1M Bis Tris Propane pH7.5 0.2M Sodium nitrate 20% w/v PEG3350 10%v/v Ethylene glycol
|
Resolution 2.80 Å R-free 0.247 |
| 8WWC De novo design binder of HRAS -120-4 Deposited 2023-10-25 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
1–166（166 aa）
|
Not recorded | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；289 K；0.1M Bis Tris Propane pH7.5 0.2M Sodium nitrate 20% w/v PEG3350 10%v/v Ethylene glycol
|
Resolution 2.80 Å R-free 0.247 |
| 9P46 Crystal structure of HRAS-G12D/Q95H (GMPPNP-bound) in complex with BBO-11818 Deposited 2025-06-16 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–169（169 aa）
|
Mutation：G12D, Q95H, C118S | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 A1CG4 methyl (3S)-3-{[(7P)-7-(2-amino-3-cyano-7-fluoro-1-benzothiophen-4-yl)-8-fluoro-2-{[(2R,4R,7aS)-2-fluorotetrahydro-1H-pyrrolizin-7a(5H)-yl]methoxy}-6-(trifluoromethyl)quinazolin-4-yl](ethyl)amino}pyrrolidine-1-carboxylate × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；0.1 M Bis-Tris pH 5.5, 25% PEG 3350, 0.2 M MgCl2
|
Resolution 2.02 Å R-free 0.228 |
| 9P46 Crystal structure of HRAS-G12D/Q95H (GMPPNP-bound) in complex with BBO-11818 Deposited 2025-06-16 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
1–169（169 aa）
|
Mutation：G12D, Q95H, C118S | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 A1CG4 methyl (3S)-3-{[(7P)-7-(2-amino-3-cyano-7-fluoro-1-benzothiophen-4-yl)-8-fluoro-2-{[(2R,4R,7aS)-2-fluorotetrahydro-1H-pyrrolizin-7a(5H)-yl]methoxy}-6-(trifluoromethyl)quinazolin-4-yl](ethyl)amino}pyrrolidine-1-carboxylate × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；0.1 M Bis-Tris pH 5.5, 25% PEG 3350, 0.2 M MgCl2
|
Resolution 2.02 Å R-free 0.228 |
| 9PU1 HRAS complex with UM0140401 compound Deposited 2025-07-30 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–166（166 aa）
|
Not recorded | GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293.15 K；Tris
|
Resolution 1.40 Å R-free 0.177 |
| 9PU3 HRAS complex with UM0140692 compound Deposited 2025-07-30 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–166（166 aa）
|
Not recorded | GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；Tris
|
Resolution 1.70 Å R-free 0.217 |
| 9PU8 HRAS complex with UM0140693 compound Deposited 2025-07-30 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–165（165 aa）
|
Not recorded | GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；Tris
|
Resolution 1.40 Å R-free 0.179 |
| 9PUL HRAS complex with UM0143525 compound Deposited 2025-07-31 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–166（166 aa）
|
Not recorded | MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；293.15 K；0.1M Tris, 0.2 M CaCl2, 18 % PEG 4,000
|
Resolution 2.50 Å R-free 0.267 |
| 9PUL HRAS complex with UM0143525 compound Deposited 2025-07-31 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
1–166（166 aa）
|
Not recorded | MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；293.15 K；0.1M Tris, 0.2 M CaCl2, 18 % PEG 4,000
|
Resolution 2.50 Å R-free 0.267 |
| 9PUL HRAS complex with UM0143525 compound Deposited 2025-07-31 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
1–166（166 aa）
|
Not recorded | MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；293.15 K；0.1M Tris, 0.2 M CaCl2, 18 % PEG 4,000
|
Resolution 2.50 Å R-free 0.267 |
| 9PUL HRAS complex with UM0143525 compound Deposited 2025-07-31 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain D
1–166（166 aa）
|
Not recorded | MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；293.15 K；0.1M Tris, 0.2 M CaCl2, 18 % PEG 4,000
|
Resolution 2.50 Å R-free 0.267 |
| 9PUN HRAS complex with UM0148697 compound Deposited 2025-07-31 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
|
Not recorded | GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.4；293.15 K；0.1 M Tris, 0.2 M MgCl2, 26 % PEG 3,350
|
Resolution 2.40 Å R-free 0.260 |
| 9PUN HRAS complex with UM0148697 compound Deposited 2025-07-31 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
1–166（166 aa）
|
Not recorded | GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.4；293.15 K；0.1 M Tris, 0.2 M MgCl2, 26 % PEG 3,350
|
Resolution 2.40 Å R-free 0.260 |
| 9PUN HRAS complex with UM0148697 compound Deposited 2025-07-31 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
1–166（166 aa）
|
Not recorded | GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.4；293.15 K；0.1 M Tris, 0.2 M MgCl2, 26 % PEG 3,350
|
Resolution 2.40 Å R-free 0.260 |
| 9PUQ HRAS complex with UM0152248 compound Deposited 2025-07-31 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–166（166 aa）
|
Not recorded | GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；293.15 K；0.1 M Tris, 0.2 M CaCl2, 23 % PEG 4,000
|
Resolution 2.00 Å R-free 0.246 |
| 9PUQ HRAS complex with UM0152248 compound Deposited 2025-07-31 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
1–166（166 aa）
|
Not recorded | GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；293.15 K；0.1 M Tris, 0.2 M CaCl2, 23 % PEG 4,000
|
Resolution 2.00 Å R-free 0.246 |
| 9PUQ HRAS complex with UM0152248 compound Deposited 2025-07-31 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
1–166（166 aa）
|
Not recorded | GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；293.15 K；0.1 M Tris, 0.2 M CaCl2, 23 % PEG 4,000
|
Resolution 2.00 Å R-free 0.246 |
| 9PUT HRAS complex with UM0152535 compound Deposited 2025-07-31 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–166（166 aa）
|
Not recorded | GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.7；293.15 K；0.1 M Bis-Tris, 0.2 M NaCl2, 21 % PEG 3,350
|
Resolution 2.00 Å R-free 0.233 |
| 9PUT HRAS complex with UM0152535 compound Deposited 2025-07-31 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
1–166（166 aa）
|
Not recorded | GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.7；293.15 K；0.1 M Bis-Tris, 0.2 M NaCl2, 21 % PEG 3,350
|
Resolution 2.00 Å R-free 0.233 |
| 9PUZ HRAS complex with UM0153030 compound Deposited 2025-07-31 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–166（166 aa）
|
Not recorded | MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 PO4 PHOSPHATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.7；293.15 K；0.1 M Tris, 0.2 M CaCl2, 22 % PEG 4,000
|
Resolution 2.00 Å R-free 0.253 |
| 9PUZ HRAS complex with UM0153030 compound Deposited 2025-07-31 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
1–166（166 aa）
|
Not recorded | MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 PO4 PHOSPHATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.7；293.15 K；0.1 M Tris, 0.2 M CaCl2, 22 % PEG 4,000
|
Resolution 2.00 Å R-free 0.253 |
| 9PUZ HRAS complex with UM0153030 compound Deposited 2025-07-31 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
1–166（166 aa）
|
Not recorded | MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 PO4 PHOSPHATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.7；293.15 K；0.1 M Tris, 0.2 M CaCl2, 22 % PEG 4,000
|
Resolution 2.00 Å R-free 0.253 |
| 9PVE HRAS complex with UM0152533 compound Deposited 2025-08-01 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–166（166 aa）
|
Not recorded | GDP GUANOSINE-5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.3；293.15 K；0.1 M Sodium citrate, 16 % PEG 3,350
|
Resolution 2.00 Å R-free 0.242 |
| 9PVE HRAS complex with UM0152533 compound Deposited 2025-08-01 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
1–166（166 aa）
|
Not recorded | GDP GUANOSINE-5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.3；293.15 K；0.1 M Sodium citrate, 16 % PEG 3,350
|
Resolution 2.00 Å R-free 0.242 |
| 9PVE HRAS complex with UM0152533 compound Deposited 2025-08-01 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
1–166（166 aa）
|
Not recorded | GDP GUANOSINE-5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.3；293.15 K；0.1 M Sodium citrate, 16 % PEG 3,350
|
Resolution 2.00 Å R-free 0.242 |
| 9PVE HRAS complex with UM0152533 compound Deposited 2025-08-01 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain D
1–166（166 aa）
|
Not recorded | GDP GUANOSINE-5'-DIPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.3；293.15 K；0.1 M Sodium citrate, 16 % PEG 3,350
|
Resolution 2.00 Å R-free 0.242 |
| 9Z5W Crystal structure of HRAS in complex with N-LHY affibody Deposited 2025-11-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–166（166 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.80 Å R-free 0.224 |
242 other PDB entries and 324 assemblies. Open the comparison page and filter oligomeric states
View Construct and Data Evidence
| UniProt name | RASH_HUMAN |
| Isoform | — |
| PDB entities | 1 |
| Chains and sequence ranges | Author chain A； PDBConstruct 1–166； UniProt 1–166 |
