6kw4

The ClassB RSC-Nucleosome Complex

Method: ELECTRON MICROSCOPY Dmax: 281.0 Å Quality: GOOD

1. Protein Identity and Related Structures Protein Identity & Related Structures

Histone H3.2

Xenopus laevis

UniProt P84233

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–DNA Heteromer Protein × 26 DNA 2 PDB declaration: 28-meric(28) Consistent with all polymer counts Chain N; UniProt 1–136 Chain Q; UniProt 1–136 Not recorded Histone H4 × 2 (P62799) Histone H2A × 2 (Q6AZJ8) DNA 167 × 1 DNA 167 × 1 Actin-related protein 7 × 1 (Q12406) Regulator of Ty1 transposition protein 102 × 1 (P53330) Chromatin structure-remodeling complex subunit RSC7 × 1 (P32832) Chromatin structure-remodeling complex protein RSC8 × 2 (P43609) Chromatin structure-remodeling complex subunit RSC9 × 1 (Q03124) Chromatin structure-remodeling complex protein RSC6 × 1 (P25632) Chromatin structure-remodeling complex subunit SFH1 × 1 (Q06168) Chromatin structure-remodeling complex protein RSC58 × 1 (Q07979) Nuclear protein STH1/NPS1 × 3 (P32597) High temperature lethal protein 1 × 1 (Q9URQ5) Chromatin structure-remodeling complex protein RSC30 × 1 (P38781) Chromatin structure-remodeling complex protein RSC3 × 1 (Q06639) Chromatin structure-remodeling complex subunit RSC4 × 1 (Q02206) Chromatin structure-remodeling complex subunit RSC2 × 1 (Q06488) Histone H4 × 2 (A0A1L8G0X3) Actin-like protein ARP9 × 1 (Q05123) ZN ZINC ION × 1 ELECTRON MICROSCOPY cryo-EM buffer:pH 7.5 cryo-EM vitrification conditions:Cryogen ETHANE Resolution 7.55 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

230 other PDB entries and 239 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name H32_XENLA
Isoform
PDB entities 1
Chains and sequence ranges Author chain N; PDBConstruct 1–136; UniProt 1–136 Author chain Q; PDBConstruct 1–136; UniProt 1–136

Histone H4

Xenopus laevis

UniProt P62799

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–DNA Heteromer Protein × 26 DNA 2 PDB declaration: 28-meric(28) Consistent with all polymer counts Chain B; UniProt 1–103 Chain R; UniProt 1–103 Not recorded Histone H3.2 × 2 (P84233) Histone H2A × 2 (Q6AZJ8) DNA 167 × 1 DNA 167 × 1 Actin-related protein 7 × 1 (Q12406) Regulator of Ty1 transposition protein 102 × 1 (P53330) Chromatin structure-remodeling complex subunit RSC7 × 1 (P32832) Chromatin structure-remodeling complex protein RSC8 × 2 (P43609) Chromatin structure-remodeling complex subunit RSC9 × 1 (Q03124) Chromatin structure-remodeling complex protein RSC6 × 1 (P25632) Chromatin structure-remodeling complex subunit SFH1 × 1 (Q06168) Chromatin structure-remodeling complex protein RSC58 × 1 (Q07979) Nuclear protein STH1/NPS1 × 3 (P32597) High temperature lethal protein 1 × 1 (Q9URQ5) Chromatin structure-remodeling complex protein RSC30 × 1 (P38781) Chromatin structure-remodeling complex protein RSC3 × 1 (Q06639) Chromatin structure-remodeling complex subunit RSC4 × 1 (Q02206) Chromatin structure-remodeling complex subunit RSC2 × 1 (Q06488) Histone H4 × 2 (A0A1L8G0X3) Actin-like protein ARP9 × 1 (Q05123) ZN ZINC ION × 1 ELECTRON MICROSCOPY cryo-EM buffer:pH 7.5 cryo-EM vitrification conditions:Cryogen ETHANE Resolution 7.55 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

354 other PDB entries and 370 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name H4_XENLA
Isoform
PDB entities 2
Chains and sequence ranges Author chain B; PDBConstruct 1–103; UniProt 1–103 Author chain R; PDBConstruct 1–103; UniProt 1–103

Histone H2A

Xenopus laevis

UniProt Q6AZJ8

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–DNA Heteromer Protein × 26 DNA 2 PDB declaration: 28-meric(28) Consistent with all polymer counts Chain O; UniProt 1–130 Chain S; UniProt 1–130 Not recorded Histone H3.2 × 2 (P84233) Histone H4 × 2 (P62799) DNA 167 × 1 DNA 167 × 1 Actin-related protein 7 × 1 (Q12406) Regulator of Ty1 transposition protein 102 × 1 (P53330) Chromatin structure-remodeling complex subunit RSC7 × 1 (P32832) Chromatin structure-remodeling complex protein RSC8 × 2 (P43609) Chromatin structure-remodeling complex subunit RSC9 × 1 (Q03124) Chromatin structure-remodeling complex protein RSC6 × 1 (P25632) Chromatin structure-remodeling complex subunit SFH1 × 1 (Q06168) Chromatin structure-remodeling complex protein RSC58 × 1 (Q07979) Nuclear protein STH1/NPS1 × 3 (P32597) High temperature lethal protein 1 × 1 (Q9URQ5) Chromatin structure-remodeling complex protein RSC30 × 1 (P38781) Chromatin structure-remodeling complex protein RSC3 × 1 (Q06639) Chromatin structure-remodeling complex subunit RSC4 × 1 (Q02206) Chromatin structure-remodeling complex subunit RSC2 × 1 (Q06488) Histone H4 × 2 (A0A1L8G0X3) Actin-like protein ARP9 × 1 (Q05123) ZN ZINC ION × 1 ELECTRON MICROSCOPY cryo-EM buffer:pH 7.5 cryo-EM vitrification conditions:Cryogen ETHANE Resolution 7.55 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

226 other PDB entries and 238 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name Q6AZJ8_XENLA
Isoform
PDB entities 3
Chains and sequence ranges Author chain O; PDBConstruct 1–130; UniProt 1–130 Author chain S; PDBConstruct 1–130; UniProt 1–130

Actin-related protein 7

OrganismNot specified

UniProt Q12406

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–DNA Heteromer Protein × 26 DNA 2 PDB declaration: 28-meric(28) Consistent with all polymer counts Chain f; UniProt 1–477 Not recorded Histone H3.2 × 2 (P84233) Histone H4 × 2 (P62799) Histone H2A × 2 (Q6AZJ8) DNA 167 × 1 DNA 167 × 1 Regulator of Ty1 transposition protein 102 × 1 (P53330) Chromatin structure-remodeling complex subunit RSC7 × 1 (P32832) Chromatin structure-remodeling complex protein RSC8 × 2 (P43609) Chromatin structure-remodeling complex subunit RSC9 × 1 (Q03124) Chromatin structure-remodeling complex protein RSC6 × 1 (P25632) Chromatin structure-remodeling complex subunit SFH1 × 1 (Q06168) Chromatin structure-remodeling complex protein RSC58 × 1 (Q07979) Nuclear protein STH1/NPS1 × 3 (P32597) High temperature lethal protein 1 × 1 (Q9URQ5) Chromatin structure-remodeling complex protein RSC30 × 1 (P38781) Chromatin structure-remodeling complex protein RSC3 × 1 (Q06639) Chromatin structure-remodeling complex subunit RSC4 × 1 (Q02206) Chromatin structure-remodeling complex subunit RSC2 × 1 (Q06488) Histone H4 × 2 (A0A1L8G0X3) Actin-like protein ARP9 × 1 (Q05123) ZN ZINC ION × 1 ELECTRON MICROSCOPY cryo-EM buffer:pH 7.5 cryo-EM vitrification conditions:Cryogen ETHANE Resolution 7.55 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

12 other PDB entries and 14 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name ARP7_YEAST
Isoform
PDB entities 6
Chains and sequence ranges Author chain f; PDBConstruct 1–477; UniProt 1–477

Regulator of Ty1 transposition protein 102

OrganismNot specified

UniProt P53330

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–DNA Heteromer Protein × 26 DNA 2 PDB declaration: 28-meric(28) Consistent with all polymer counts Chain h; UniProt 1–157 Not recorded Histone H3.2 × 2 (P84233) Histone H4 × 2 (P62799) Histone H2A × 2 (Q6AZJ8) DNA 167 × 1 DNA 167 × 1 Actin-related protein 7 × 1 (Q12406) Chromatin structure-remodeling complex subunit RSC7 × 1 (P32832) Chromatin structure-remodeling complex protein RSC8 × 2 (P43609) Chromatin structure-remodeling complex subunit RSC9 × 1 (Q03124) Chromatin structure-remodeling complex protein RSC6 × 1 (P25632) Chromatin structure-remodeling complex subunit SFH1 × 1 (Q06168) Chromatin structure-remodeling complex protein RSC58 × 1 (Q07979) Nuclear protein STH1/NPS1 × 3 (P32597) High temperature lethal protein 1 × 1 (Q9URQ5) Chromatin structure-remodeling complex protein RSC30 × 1 (P38781) Chromatin structure-remodeling complex protein RSC3 × 1 (Q06639) Chromatin structure-remodeling complex subunit RSC4 × 1 (Q02206) Chromatin structure-remodeling complex subunit RSC2 × 1 (Q06488) Histone H4 × 2 (A0A1L8G0X3) Actin-like protein ARP9 × 1 (Q05123) ZN ZINC ION × 1 ELECTRON MICROSCOPY cryo-EM buffer:pH 7.5 cryo-EM vitrification conditions:Cryogen ETHANE Resolution 7.55 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

11 other PDB entries and 13 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RT102_YEAST
Isoform
PDB entities 7
Chains and sequence ranges Author chain h; PDBConstruct 1–157; UniProt 1–157

Chromatin structure-remodeling complex subunit RSC7

OrganismNot specified

UniProt P32832

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–DNA Heteromer Protein × 26 DNA 2 PDB declaration: 28-meric(28) Consistent with all polymer counts Chain F; UniProt 1–435 Not recorded Histone H3.2 × 2 (P84233) Histone H4 × 2 (P62799) Histone H2A × 2 (Q6AZJ8) DNA 167 × 1 DNA 167 × 1 Actin-related protein 7 × 1 (Q12406) Regulator of Ty1 transposition protein 102 × 1 (P53330) Chromatin structure-remodeling complex protein RSC8 × 2 (P43609) Chromatin structure-remodeling complex subunit RSC9 × 1 (Q03124) Chromatin structure-remodeling complex protein RSC6 × 1 (P25632) Chromatin structure-remodeling complex subunit SFH1 × 1 (Q06168) Chromatin structure-remodeling complex protein RSC58 × 1 (Q07979) Nuclear protein STH1/NPS1 × 3 (P32597) High temperature lethal protein 1 × 1 (Q9URQ5) Chromatin structure-remodeling complex protein RSC30 × 1 (P38781) Chromatin structure-remodeling complex protein RSC3 × 1 (Q06639) Chromatin structure-remodeling complex subunit RSC4 × 1 (Q02206) Chromatin structure-remodeling complex subunit RSC2 × 1 (Q06488) Histone H4 × 2 (A0A1L8G0X3) Actin-like protein ARP9 × 1 (Q05123) ZN ZINC ION × 1 ELECTRON MICROSCOPY cryo-EM buffer:pH 7.5 cryo-EM vitrification conditions:Cryogen ETHANE Resolution 7.55 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

6 other PDB entries and 6 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RSC7_YEAST
Isoform
PDB entities 8
Chains and sequence ranges Author chain F; PDBConstruct 1–435; UniProt 1–435

Chromatin structure-remodeling complex protein RSC8

OrganismNot specified

UniProt P43609

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–DNA Heteromer Protein × 26 DNA 2 PDB declaration: 28-meric(28) Consistent with all polymer counts Chain D; UniProt 1–557 Chain H; UniProt 1–557 Not recorded Histone H3.2 × 2 (P84233) Histone H4 × 2 (P62799) Histone H2A × 2 (Q6AZJ8) DNA 167 × 1 DNA 167 × 1 Actin-related protein 7 × 1 (Q12406) Regulator of Ty1 transposition protein 102 × 1 (P53330) Chromatin structure-remodeling complex subunit RSC7 × 1 (P32832) Chromatin structure-remodeling complex subunit RSC9 × 1 (Q03124) Chromatin structure-remodeling complex protein RSC6 × 1 (P25632) Chromatin structure-remodeling complex subunit SFH1 × 1 (Q06168) Chromatin structure-remodeling complex protein RSC58 × 1 (Q07979) Nuclear protein STH1/NPS1 × 3 (P32597) High temperature lethal protein 1 × 1 (Q9URQ5) Chromatin structure-remodeling complex protein RSC30 × 1 (P38781) Chromatin structure-remodeling complex protein RSC3 × 1 (Q06639) Chromatin structure-remodeling complex subunit RSC4 × 1 (Q02206) Chromatin structure-remodeling complex subunit RSC2 × 1 (Q06488) Histone H4 × 2 (A0A1L8G0X3) Actin-like protein ARP9 × 1 (Q05123) ZN ZINC ION × 1 ELECTRON MICROSCOPY cryo-EM buffer:pH 7.5 cryo-EM vitrification conditions:Cryogen ETHANE Resolution 7.55 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

6 other PDB entries and 6 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RSC8_YEAST
Isoform
PDB entities 9
Chains and sequence ranges Author chain D; PDBConstruct 1–557; UniProt 1–557 Author chain H; PDBConstruct 1–557; UniProt 1–557

Chromatin structure-remodeling complex subunit RSC9

OrganismNot specified

UniProt Q03124

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–DNA Heteromer Protein × 26 DNA 2 PDB declaration: 28-meric(28) Consistent with all polymer counts Chain M; UniProt 1–581 Not recorded Histone H3.2 × 2 (P84233) Histone H4 × 2 (P62799) Histone H2A × 2 (Q6AZJ8) DNA 167 × 1 DNA 167 × 1 Actin-related protein 7 × 1 (Q12406) Regulator of Ty1 transposition protein 102 × 1 (P53330) Chromatin structure-remodeling complex subunit RSC7 × 1 (P32832) Chromatin structure-remodeling complex protein RSC8 × 2 (P43609) Chromatin structure-remodeling complex protein RSC6 × 1 (P25632) Chromatin structure-remodeling complex subunit SFH1 × 1 (Q06168) Chromatin structure-remodeling complex protein RSC58 × 1 (Q07979) Nuclear protein STH1/NPS1 × 3 (P32597) High temperature lethal protein 1 × 1 (Q9URQ5) Chromatin structure-remodeling complex protein RSC30 × 1 (P38781) Chromatin structure-remodeling complex protein RSC3 × 1 (Q06639) Chromatin structure-remodeling complex subunit RSC4 × 1 (Q02206) Chromatin structure-remodeling complex subunit RSC2 × 1 (Q06488) Histone H4 × 2 (A0A1L8G0X3) Actin-like protein ARP9 × 1 (Q05123) ZN ZINC ION × 1 ELECTRON MICROSCOPY cryo-EM buffer:pH 7.5 cryo-EM vitrification conditions:Cryogen ETHANE Resolution 7.55 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

6 other PDB entries and 6 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RSC9_YEAST
Isoform
PDB entities 10
Chains and sequence ranges Author chain M; PDBConstruct 1–581; UniProt 1–581

Chromatin structure-remodeling complex protein RSC6

OrganismNot specified

UniProt P25632

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–DNA Heteromer Protein × 26 DNA 2 PDB declaration: 28-meric(28) Consistent with all polymer counts Chain I; UniProt 1–483 Not recorded Histone H3.2 × 2 (P84233) Histone H4 × 2 (P62799) Histone H2A × 2 (Q6AZJ8) DNA 167 × 1 DNA 167 × 1 Actin-related protein 7 × 1 (Q12406) Regulator of Ty1 transposition protein 102 × 1 (P53330) Chromatin structure-remodeling complex subunit RSC7 × 1 (P32832) Chromatin structure-remodeling complex protein RSC8 × 2 (P43609) Chromatin structure-remodeling complex subunit RSC9 × 1 (Q03124) Chromatin structure-remodeling complex subunit SFH1 × 1 (Q06168) Chromatin structure-remodeling complex protein RSC58 × 1 (Q07979) Nuclear protein STH1/NPS1 × 3 (P32597) High temperature lethal protein 1 × 1 (Q9URQ5) Chromatin structure-remodeling complex protein RSC30 × 1 (P38781) Chromatin structure-remodeling complex protein RSC3 × 1 (Q06639) Chromatin structure-remodeling complex subunit RSC4 × 1 (Q02206) Chromatin structure-remodeling complex subunit RSC2 × 1 (Q06488) Histone H4 × 2 (A0A1L8G0X3) Actin-like protein ARP9 × 1 (Q05123) ZN ZINC ION × 1 ELECTRON MICROSCOPY cryo-EM buffer:pH 7.5 cryo-EM vitrification conditions:Cryogen ETHANE Resolution 7.55 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

6 other PDB entries and 6 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RSC6_YEAST
Isoform
PDB entities 11
Chains and sequence ranges Author chain I; PDBConstruct 1–483; UniProt 1–483

Chromatin structure-remodeling complex subunit SFH1

OrganismNot specified

UniProt Q06168

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–DNA Heteromer Protein × 26 DNA 2 PDB declaration: 28-meric(28) Consistent with all polymer counts Chain G; UniProt 1–426 Not recorded Histone H3.2 × 2 (P84233) Histone H4 × 2 (P62799) Histone H2A × 2 (Q6AZJ8) DNA 167 × 1 DNA 167 × 1 Actin-related protein 7 × 1 (Q12406) Regulator of Ty1 transposition protein 102 × 1 (P53330) Chromatin structure-remodeling complex subunit RSC7 × 1 (P32832) Chromatin structure-remodeling complex protein RSC8 × 2 (P43609) Chromatin structure-remodeling complex subunit RSC9 × 1 (Q03124) Chromatin structure-remodeling complex protein RSC6 × 1 (P25632) Chromatin structure-remodeling complex protein RSC58 × 1 (Q07979) Nuclear protein STH1/NPS1 × 3 (P32597) High temperature lethal protein 1 × 1 (Q9URQ5) Chromatin structure-remodeling complex protein RSC30 × 1 (P38781) Chromatin structure-remodeling complex protein RSC3 × 1 (Q06639) Chromatin structure-remodeling complex subunit RSC4 × 1 (Q02206) Chromatin structure-remodeling complex subunit RSC2 × 1 (Q06488) Histone H4 × 2 (A0A1L8G0X3) Actin-like protein ARP9 × 1 (Q05123) ZN ZINC ION × 1 ELECTRON MICROSCOPY cryo-EM buffer:pH 7.5 cryo-EM vitrification conditions:Cryogen ETHANE Resolution 7.55 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

6 other PDB entries and 6 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name SFH1_YEAST
Isoform
PDB entities 12
Chains and sequence ranges Author chain G; PDBConstruct 1–426; UniProt 1–426

Chromatin structure-remodeling complex protein RSC58

OrganismNot specified

UniProt Q07979

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–DNA Heteromer Protein × 26 DNA 2 PDB declaration: 28-meric(28) Consistent with all polymer counts Chain A; UniProt 1–502 Not recorded Histone H3.2 × 2 (P84233) Histone H4 × 2 (P62799) Histone H2A × 2 (Q6AZJ8) DNA 167 × 1 DNA 167 × 1 Actin-related protein 7 × 1 (Q12406) Regulator of Ty1 transposition protein 102 × 1 (P53330) Chromatin structure-remodeling complex subunit RSC7 × 1 (P32832) Chromatin structure-remodeling complex protein RSC8 × 2 (P43609) Chromatin structure-remodeling complex subunit RSC9 × 1 (Q03124) Chromatin structure-remodeling complex protein RSC6 × 1 (P25632) Chromatin structure-remodeling complex subunit SFH1 × 1 (Q06168) Nuclear protein STH1/NPS1 × 3 (P32597) High temperature lethal protein 1 × 1 (Q9URQ5) Chromatin structure-remodeling complex protein RSC30 × 1 (P38781) Chromatin structure-remodeling complex protein RSC3 × 1 (Q06639) Chromatin structure-remodeling complex subunit RSC4 × 1 (Q02206) Chromatin structure-remodeling complex subunit RSC2 × 1 (Q06488) Histone H4 × 2 (A0A1L8G0X3) Actin-like protein ARP9 × 1 (Q05123) ZN ZINC ION × 1 ELECTRON MICROSCOPY cryo-EM buffer:pH 7.5 cryo-EM vitrification conditions:Cryogen ETHANE Resolution 7.55 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

6 other PDB entries and 6 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RSC58_YEAST
Isoform
PDB entities 13
Chains and sequence ranges Author chain A; PDBConstruct 1–502; UniProt 1–502

Nuclear protein STH1/NPS1

OrganismNot specified

UniProt P32597

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–DNA Heteromer Protein × 26 DNA 2 PDB declaration: 28-meric(28) Consistent with all polymer counts Chain J; UniProt 1–1359 Chain V; UniProt 1–1359 Chain Y; UniProt 1–1359 Not recorded Histone H3.2 × 2 (P84233) Histone H4 × 2 (P62799) Histone H2A × 2 (Q6AZJ8) DNA 167 × 1 DNA 167 × 1 Actin-related protein 7 × 1 (Q12406) Regulator of Ty1 transposition protein 102 × 1 (P53330) Chromatin structure-remodeling complex subunit RSC7 × 1 (P32832) Chromatin structure-remodeling complex protein RSC8 × 2 (P43609) Chromatin structure-remodeling complex subunit RSC9 × 1 (Q03124) Chromatin structure-remodeling complex protein RSC6 × 1 (P25632) Chromatin structure-remodeling complex subunit SFH1 × 1 (Q06168) Chromatin structure-remodeling complex protein RSC58 × 1 (Q07979) High temperature lethal protein 1 × 1 (Q9URQ5) Chromatin structure-remodeling complex protein RSC30 × 1 (P38781) Chromatin structure-remodeling complex protein RSC3 × 1 (Q06639) Chromatin structure-remodeling complex subunit RSC4 × 1 (Q02206) Chromatin structure-remodeling complex subunit RSC2 × 1 (Q06488) Histone H4 × 2 (A0A1L8G0X3) Actin-like protein ARP9 × 1 (Q05123) ZN ZINC ION × 1 ELECTRON MICROSCOPY cryo-EM buffer:pH 7.5 cryo-EM vitrification conditions:Cryogen ETHANE Resolution 7.55 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

14 other PDB entries and 33 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name STH1_YEAST
Isoform
PDB entities 14
Chains and sequence ranges Author chain J; PDBConstruct 1–1359; UniProt 1–1359 Author chain V; PDBConstruct 1–1359; UniProt 1–1359 Author chain Y; PDBConstruct 1–1359; UniProt 1–1359

High temperature lethal protein 1

OrganismNot specified

UniProt Q9URQ5

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–DNA Heteromer Protein × 26 DNA 2 PDB declaration: 28-meric(28) Consistent with all polymer counts Chain E; UniProt 1–78 Not recorded Histone H3.2 × 2 (P84233) Histone H4 × 2 (P62799) Histone H2A × 2 (Q6AZJ8) DNA 167 × 1 DNA 167 × 1 Actin-related protein 7 × 1 (Q12406) Regulator of Ty1 transposition protein 102 × 1 (P53330) Chromatin structure-remodeling complex subunit RSC7 × 1 (P32832) Chromatin structure-remodeling complex protein RSC8 × 2 (P43609) Chromatin structure-remodeling complex subunit RSC9 × 1 (Q03124) Chromatin structure-remodeling complex protein RSC6 × 1 (P25632) Chromatin structure-remodeling complex subunit SFH1 × 1 (Q06168) Chromatin structure-remodeling complex protein RSC58 × 1 (Q07979) Nuclear protein STH1/NPS1 × 3 (P32597) Chromatin structure-remodeling complex protein RSC30 × 1 (P38781) Chromatin structure-remodeling complex protein RSC3 × 1 (Q06639) Chromatin structure-remodeling complex subunit RSC4 × 1 (Q02206) Chromatin structure-remodeling complex subunit RSC2 × 1 (Q06488) Histone H4 × 2 (A0A1L8G0X3) Actin-like protein ARP9 × 1 (Q05123) ZN ZINC ION × 1 ELECTRON MICROSCOPY cryo-EM buffer:pH 7.5 cryo-EM vitrification conditions:Cryogen ETHANE Resolution 7.55 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

6 other PDB entries and 6 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name HTL1_YEAST
Isoform
PDB entities 15
Chains and sequence ranges Author chain E; PDBConstruct 1–78; UniProt 1–78

Chromatin structure-remodeling complex protein RSC30

OrganismNot specified

UniProt P38781

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–DNA Heteromer Protein × 26 DNA 2 PDB declaration: 28-meric(28) Consistent with all polymer counts Chain C; UniProt 1–883 Not recorded Histone H3.2 × 2 (P84233) Histone H4 × 2 (P62799) Histone H2A × 2 (Q6AZJ8) DNA 167 × 1 DNA 167 × 1 Actin-related protein 7 × 1 (Q12406) Regulator of Ty1 transposition protein 102 × 1 (P53330) Chromatin structure-remodeling complex subunit RSC7 × 1 (P32832) Chromatin structure-remodeling complex protein RSC8 × 2 (P43609) Chromatin structure-remodeling complex subunit RSC9 × 1 (Q03124) Chromatin structure-remodeling complex protein RSC6 × 1 (P25632) Chromatin structure-remodeling complex subunit SFH1 × 1 (Q06168) Chromatin structure-remodeling complex protein RSC58 × 1 (Q07979) Nuclear protein STH1/NPS1 × 3 (P32597) High temperature lethal protein 1 × 1 (Q9URQ5) Chromatin structure-remodeling complex protein RSC3 × 1 (Q06639) Chromatin structure-remodeling complex subunit RSC4 × 1 (Q02206) Chromatin structure-remodeling complex subunit RSC2 × 1 (Q06488) Histone H4 × 2 (A0A1L8G0X3) Actin-like protein ARP9 × 1 (Q05123) ZN ZINC ION × 1 ELECTRON MICROSCOPY cryo-EM buffer:pH 7.5 cryo-EM vitrification conditions:Cryogen ETHANE Resolution 7.55 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

5 other PDB entries and 5 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RSC30_YEAST
Isoform
PDB entities 16
Chains and sequence ranges Author chain C; PDBConstruct 1–883; UniProt 1–883

Chromatin structure-remodeling complex protein RSC3

OrganismNot specified

UniProt Q06639

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–DNA Heteromer Protein × 26 DNA 2 PDB declaration: 28-meric(28) Consistent with all polymer counts Chain K; UniProt 1–885 Not recorded Histone H3.2 × 2 (P84233) Histone H4 × 2 (P62799) Histone H2A × 2 (Q6AZJ8) DNA 167 × 1 DNA 167 × 1 Actin-related protein 7 × 1 (Q12406) Regulator of Ty1 transposition protein 102 × 1 (P53330) Chromatin structure-remodeling complex subunit RSC7 × 1 (P32832) Chromatin structure-remodeling complex protein RSC8 × 2 (P43609) Chromatin structure-remodeling complex subunit RSC9 × 1 (Q03124) Chromatin structure-remodeling complex protein RSC6 × 1 (P25632) Chromatin structure-remodeling complex subunit SFH1 × 1 (Q06168) Chromatin structure-remodeling complex protein RSC58 × 1 (Q07979) Nuclear protein STH1/NPS1 × 3 (P32597) High temperature lethal protein 1 × 1 (Q9URQ5) Chromatin structure-remodeling complex protein RSC30 × 1 (P38781) Chromatin structure-remodeling complex subunit RSC4 × 1 (Q02206) Chromatin structure-remodeling complex subunit RSC2 × 1 (Q06488) Histone H4 × 2 (A0A1L8G0X3) Actin-like protein ARP9 × 1 (Q05123) ZN ZINC ION × 1 ELECTRON MICROSCOPY cryo-EM buffer:pH 7.5 cryo-EM vitrification conditions:Cryogen ETHANE Resolution 7.55 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

5 other PDB entries and 5 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RSC3_YEAST
Isoform
PDB entities 17
Chains and sequence ranges Author chain K; PDBConstruct 1–885; UniProt 1–885

Chromatin structure-remodeling complex subunit RSC4

OrganismNot specified

UniProt Q02206

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–DNA Heteromer Protein × 26 DNA 2 PDB declaration: 28-meric(28) Consistent with all polymer counts Chain X; UniProt 1–625 Not recorded Histone H3.2 × 2 (P84233) Histone H4 × 2 (P62799) Histone H2A × 2 (Q6AZJ8) DNA 167 × 1 DNA 167 × 1 Actin-related protein 7 × 1 (Q12406) Regulator of Ty1 transposition protein 102 × 1 (P53330) Chromatin structure-remodeling complex subunit RSC7 × 1 (P32832) Chromatin structure-remodeling complex protein RSC8 × 2 (P43609) Chromatin structure-remodeling complex subunit RSC9 × 1 (Q03124) Chromatin structure-remodeling complex protein RSC6 × 1 (P25632) Chromatin structure-remodeling complex subunit SFH1 × 1 (Q06168) Chromatin structure-remodeling complex protein RSC58 × 1 (Q07979) Nuclear protein STH1/NPS1 × 3 (P32597) High temperature lethal protein 1 × 1 (Q9URQ5) Chromatin structure-remodeling complex protein RSC30 × 1 (P38781) Chromatin structure-remodeling complex protein RSC3 × 1 (Q06639) Chromatin structure-remodeling complex subunit RSC2 × 1 (Q06488) Histone H4 × 2 (A0A1L8G0X3) Actin-like protein ARP9 × 1 (Q05123) ZN ZINC ION × 1 ELECTRON MICROSCOPY cryo-EM buffer:pH 7.5 cryo-EM vitrification conditions:Cryogen ETHANE Resolution 7.55 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

10 other PDB entries and 13 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RSC4_YEAST
Isoform
PDB entities 18
Chains and sequence ranges Author chain X; PDBConstruct 1–625; UniProt 1–625

Chromatin structure-remodeling complex subunit RSC2

OrganismNot specified

UniProt Q06488

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–DNA Heteromer Protein × 26 DNA 2 PDB declaration: 28-meric(28) Consistent with all polymer counts Chain L; UniProt 1–889 Not recorded Histone H3.2 × 2 (P84233) Histone H4 × 2 (P62799) Histone H2A × 2 (Q6AZJ8) DNA 167 × 1 DNA 167 × 1 Actin-related protein 7 × 1 (Q12406) Regulator of Ty1 transposition protein 102 × 1 (P53330) Chromatin structure-remodeling complex subunit RSC7 × 1 (P32832) Chromatin structure-remodeling complex protein RSC8 × 2 (P43609) Chromatin structure-remodeling complex subunit RSC9 × 1 (Q03124) Chromatin structure-remodeling complex protein RSC6 × 1 (P25632) Chromatin structure-remodeling complex subunit SFH1 × 1 (Q06168) Chromatin structure-remodeling complex protein RSC58 × 1 (Q07979) Nuclear protein STH1/NPS1 × 3 (P32597) High temperature lethal protein 1 × 1 (Q9URQ5) Chromatin structure-remodeling complex protein RSC30 × 1 (P38781) Chromatin structure-remodeling complex protein RSC3 × 1 (Q06639) Chromatin structure-remodeling complex subunit RSC4 × 1 (Q02206) Histone H4 × 2 (A0A1L8G0X3) Actin-like protein ARP9 × 1 (Q05123) ZN ZINC ION × 1 ELECTRON MICROSCOPY cryo-EM buffer:pH 7.5 cryo-EM vitrification conditions:Cryogen ETHANE Resolution 7.55 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

6 other PDB entries and 7 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RSC2_YEAST
Isoform
PDB entities 19
Chains and sequence ranges Author chain L; PDBConstruct 1–889; UniProt 1–889

Histone H4

Xenopus laevis

UniProt A0A1L8G0X3

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–DNA Heteromer Protein × 26 DNA 2 PDB declaration: 28-meric(28) Consistent with all polymer counts Chain P; UniProt 1–126 Chain T; UniProt 1–126 Not recorded Histone H3.2 × 2 (P84233) Histone H4 × 2 (P62799) Histone H2A × 2 (Q6AZJ8) DNA 167 × 1 DNA 167 × 1 Actin-related protein 7 × 1 (Q12406) Regulator of Ty1 transposition protein 102 × 1 (P53330) Chromatin structure-remodeling complex subunit RSC7 × 1 (P32832) Chromatin structure-remodeling complex protein RSC8 × 2 (P43609) Chromatin structure-remodeling complex subunit RSC9 × 1 (Q03124) Chromatin structure-remodeling complex protein RSC6 × 1 (P25632) Chromatin structure-remodeling complex subunit SFH1 × 1 (Q06168) Chromatin structure-remodeling complex protein RSC58 × 1 (Q07979) Nuclear protein STH1/NPS1 × 3 (P32597) High temperature lethal protein 1 × 1 (Q9URQ5) Chromatin structure-remodeling complex protein RSC30 × 1 (P38781) Chromatin structure-remodeling complex protein RSC3 × 1 (Q06639) Chromatin structure-remodeling complex subunit RSC4 × 1 (Q02206) Chromatin structure-remodeling complex subunit RSC2 × 1 (Q06488) Actin-like protein ARP9 × 1 (Q05123) ZN ZINC ION × 1 ELECTRON MICROSCOPY cryo-EM buffer:pH 7.5 cryo-EM vitrification conditions:Cryogen ETHANE Resolution 7.55 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

3 other PDB entries and 3 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name A0A1L8G0X3_XENLA
Isoform
PDB entities 20
Chains and sequence ranges Author chain P; PDBConstruct 1–126; UniProt 1–126 Author chain T; PDBConstruct 1–126; UniProt 1–126

Actin-like protein ARP9

OrganismNot specified

UniProt Q05123

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–DNA Heteromer Protein × 26 DNA 2 PDB declaration: 28-meric(28) Consistent with all polymer counts Chain g; UniProt 1–467 Not recorded Histone H3.2 × 2 (P84233) Histone H4 × 2 (P62799) Histone H2A × 2 (Q6AZJ8) DNA 167 × 1 DNA 167 × 1 Actin-related protein 7 × 1 (Q12406) Regulator of Ty1 transposition protein 102 × 1 (P53330) Chromatin structure-remodeling complex subunit RSC7 × 1 (P32832) Chromatin structure-remodeling complex protein RSC8 × 2 (P43609) Chromatin structure-remodeling complex subunit RSC9 × 1 (Q03124) Chromatin structure-remodeling complex protein RSC6 × 1 (P25632) Chromatin structure-remodeling complex subunit SFH1 × 1 (Q06168) Chromatin structure-remodeling complex protein RSC58 × 1 (Q07979) Nuclear protein STH1/NPS1 × 3 (P32597) High temperature lethal protein 1 × 1 (Q9URQ5) Chromatin structure-remodeling complex protein RSC30 × 1 (P38781) Chromatin structure-remodeling complex protein RSC3 × 1 (Q06639) Chromatin structure-remodeling complex subunit RSC4 × 1 (Q02206) Chromatin structure-remodeling complex subunit RSC2 × 1 (Q06488) Histone H4 × 2 (A0A1L8G0X3) ZN ZINC ION × 1 ELECTRON MICROSCOPY cryo-EM buffer:pH 7.5 cryo-EM vitrification conditions:Cryogen ETHANE Resolution 7.55 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

12 other PDB entries and 14 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name ARP9_YEAST
Isoform
PDB entities 21
Chains and sequence ranges Author chain g; PDBConstruct 1–467; UniProt 1–467

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 6kw4

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 6kw4
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2. Structure Basics 2. Structure Basics

Entry ID entry_id6kw4
Deposition date deposition_date2019-09-06
Structure title titleThe ClassB RSC-Nucleosome Complex
Keywords keywordschromatin remodeler, SWI/SNF family, DNA BINDING PROTEIN-DNA complex; DNA BINDING PROTEIN/DNA
Experimental Method methodELECTRON MICROSCOPY

3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier72.34
Radius of gyration Rg (electron density) rg_electron72.48
Forward intensity I(0) i07038800000.00
Molecular weight molecular_weight657400.0 kDa
Excluded volume excluded_volume800590 ų
Envelope volume envelope_volume1383400 ų
Hydration-shell volume shell_volume157620 ų
Envelope diameter envelope_diameter224.8
Shell Rg shell_rg75.86
Envelope Rg envelope_rg68.75
Shape Rg shape_rg72.47
Total Rg total_rg72.57
Total atoms total_atoms45897
Residues n_residues5193
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 −1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3

4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax281.0
Rg (real space) rg_real77.32
Rg uncertainty (real space) rg_real_error2.07
I(0) (real space) i0_real7.1210e+09
I(0) uncertainty (real space) i0_real_error1.3430e+08
Rg (reciprocal space) rg_reciprocal72.95
I(0) (reciprocal space) i0_reciprocal7049000000.0000
Solution quality estimate total_estimate0.8765
Solution quality rating solution_quality GOOD a GOOD solution
P(r) peaks n_peaks1
Primary peak position r_peak_primary89.4
Skewness Skewness skewness0.610
Kurtosis Kurtosis kurtosis0.628
Angular range angular_range— – 0.1100 −1
Current regularization parameter α current_alpha0.8092
Highest regularization parameter α highest_alpha249600000.0000
Real-space data points n_real_points23
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.684; Stabil: 0.830; Sysdev: 1.000; Positv: 1.000; Valcen: 0.977; Smooth: 0.912

5. Crystallography and Experiment 5. Crystallography & Experiment

6. Entities and Polymers Entities & Polymers (22)

8. Citations (1)

9. Files and Curves (10)