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9cwt

Human kidney respiratory complex I

Method: ELECTRON MICROSCOPY Dmax: 277.1 Å Quality: GOOD
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1. Protein Identity and Related Structures Protein Identity & Related Structures

NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial

OrganismNot specified

UniProt P49821

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain A； UniProt 1–464 Not recorded NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （O00217） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （O75251） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （P56556） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （O43678） Acyl carrier protein, mitochondrial × 2 （O14561） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q16718） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （O95182） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q16795） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （O43181） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P28331） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q9UI09） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P19404） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （O75489） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （O75306） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O15239） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （O75380） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （O95167） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q86Y39） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9P0J0） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （O95178） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （O43676） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （O43674） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （O95139） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （O95169） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （O96000） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q9NX14） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （O43677） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （O95298） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （O43920） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03897） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03901） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03915） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03923） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （O75438） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （O95168） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9Y6M9） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03905） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03886） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P51970） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （P17568） Unknow peptide × 1 NADH-ubiquinone oxidoreductase chain 2 × 1 （P03891） SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.44 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9CWT Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5XTB Cryo-EM structure of human respiratory complex I matrix arm Deposited 2017-06-18 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain A 27–457（431 aa） Fragment：UNP RESIDUES 27-457
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5XTD Cryo-EM structure of human respiratory complex I Deposited 2017-06-19 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain A 27–457（431 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 9 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 CDL CARDIOLIPIN × 5 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
5XTH Cryo-EM structure of human respiratory supercomplex I1III2IV1 Deposited 2017-06-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain A 27–457（431 aa） Fragment：UNP RESIDUES 27-457
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 12 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 4 CDL CARDIOLIPIN × 14 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 10 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 HEA HEME-A × 2 ZN ZINC ION × 1 HEC HEME C × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
5XTI Cryo-EM architecture of human respiratory chain megacomplex-I2III2IV2 Deposited 2017-06-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 138 PDB declaration: 138-meric
Chain A 27–457（431 aa） Fragment：UNP RESIDUES 27-457
Chain BA 27–457（431 aa） Fragment：UNP RESIDUES 27-457
Not recorded SF4 IRON/SULFUR CLUSTER × 12 FMN FLAVIN MONONUCLEOTIDE × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 21 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 4 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 6 CDL CARDIOLIPIN × 19 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 14 CU COPPER (II) ION × 6 MG MAGNESIUM ION × 2 HEA HEME-A × 4 ZN ZINC ION × 2 HEC HEME C × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 17.40 Å
9I4I High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-01-24 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain C 1–464（464 aa）
Not recorded PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 CDL CARDIOLIPIN × 6 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.63 Å
9TI4 High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-12-04 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain C 27–460（434 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 ZN ZINC ION × 1 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；25 mM HEPES-KOH pH=7.5, 50 mM KCl, 20 mM Mg(OAc)2, 0.01% (v/v) Lauryl Maltose Neopentyl Glycol (LMNG), 0.001 % cardiolipin, 0.001 % glycol-diosgenin (GDN, Sigma-Aldrich), 0.1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.66 Å

6 other PDB entries and 6 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUV1_HUMAN
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 1–464； UniProt 1–464

NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial

OrganismNot specified

UniProt O00217

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain B； UniProt 1–210 Not recorded NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P49821） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （O75251） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （P56556） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （O43678） Acyl carrier protein, mitochondrial × 2 （O14561） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q16718） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （O95182） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q16795） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （O43181） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P28331） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q9UI09） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P19404） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （O75489） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （O75306） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O15239） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （O75380） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （O95167） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q86Y39） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9P0J0） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （O95178） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （O43676） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （O43674） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （O95139） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （O95169） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （O96000） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q9NX14） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （O43677） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （O95298） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （O43920） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03897） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03901） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03915） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03923） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （O75438） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （O95168） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9Y6M9） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03905） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03886） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P51970） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （P17568） Unknow peptide × 1 NADH-ubiquinone oxidoreductase chain 2 × 1 （P03891） SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.44 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9CWT Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5XTB Cryo-EM structure of human respiratory complex I matrix arm Deposited 2017-06-18 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain B 35–210（176 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5XTD Cryo-EM structure of human respiratory complex I Deposited 2017-06-19 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain B 35–210（176 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 9 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 CDL CARDIOLIPIN × 5 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
5XTH Cryo-EM structure of human respiratory supercomplex I1III2IV1 Deposited 2017-06-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain B 35–210（176 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 12 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 4 CDL CARDIOLIPIN × 14 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 10 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 HEA HEME-A × 2 ZN ZINC ION × 1 HEC HEME C × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
5XTI Cryo-EM architecture of human respiratory chain megacomplex-I2III2IV2 Deposited 2017-06-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 138 PDB declaration: 138-meric
Chain B 35–210（176 aa）
Chain BB 35–210（176 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 12 FMN FLAVIN MONONUCLEOTIDE × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 21 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 4 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 6 CDL CARDIOLIPIN × 19 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 14 CU COPPER (II) ION × 6 MG MAGNESIUM ION × 2 HEA HEME-A × 4 ZN ZINC ION × 2 HEC HEME C × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 17.40 Å
9I4I High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-01-24 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain D 1–210（210 aa）
Not recorded PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 CDL CARDIOLIPIN × 6 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.63 Å
9TI4 High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-12-04 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain D 35–210（176 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 ZN ZINC ION × 1 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；25 mM HEPES-KOH pH=7.5, 50 mM KCl, 20 mM Mg(OAc)2, 0.01% (v/v) Lauryl Maltose Neopentyl Glycol (LMNG), 0.001 % cardiolipin, 0.001 % glycol-diosgenin (GDN, Sigma-Aldrich), 0.1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.66 Å

6 other PDB entries and 6 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUS8_HUMAN
Isoform —
PDB entities 2
Chains and sequence ranges Author chain B； PDBConstruct 1–210； UniProt 1–210

NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial

OrganismNot specified

UniProt O75251

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain C； UniProt 1–213 Not recorded NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P49821） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （O00217） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （P56556） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （O43678） Acyl carrier protein, mitochondrial × 2 （O14561） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q16718） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （O95182） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q16795） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （O43181） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P28331） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q9UI09） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P19404） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （O75489） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （O75306） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O15239） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （O75380） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （O95167） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q86Y39） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9P0J0） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （O95178） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （O43676） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （O43674） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （O95139） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （O95169） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （O96000） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q9NX14） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （O43677） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （O95298） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （O43920） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03897） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03901） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03915） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03923） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （O75438） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （O95168） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9Y6M9） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03905） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03886） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P51970） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （P17568） Unknow peptide × 1 NADH-ubiquinone oxidoreductase chain 2 × 1 （P03891） SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.44 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9CWT Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5XTB Cryo-EM structure of human respiratory complex I matrix arm Deposited 2017-06-18 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain C 58–213（156 aa） Fragment：UNP RESIDUES 58-213
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5XTD Cryo-EM structure of human respiratory complex I Deposited 2017-06-19 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain C 58–213（156 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 9 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 CDL CARDIOLIPIN × 5 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
5XTH Cryo-EM structure of human respiratory supercomplex I1III2IV1 Deposited 2017-06-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain C 58–213（156 aa） Fragment：UNP RESIDUES 58-213
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 12 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 4 CDL CARDIOLIPIN × 14 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 10 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 HEA HEME-A × 2 ZN ZINC ION × 1 HEC HEME C × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
5XTI Cryo-EM architecture of human respiratory chain megacomplex-I2III2IV2 Deposited 2017-06-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 138 PDB declaration: 138-meric
Chain BC 58–213（156 aa） Fragment：UNP RESIDUES 58-213
Chain C 58–213（156 aa） Fragment：UNP RESIDUES 58-213
Not recorded SF4 IRON/SULFUR CLUSTER × 12 FMN FLAVIN MONONUCLEOTIDE × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 21 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 4 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 6 CDL CARDIOLIPIN × 19 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 14 CU COPPER (II) ION × 6 MG MAGNESIUM ION × 2 HEA HEME-A × 4 ZN ZINC ION × 2 HEC HEME C × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 17.40 Å
9I4I High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-01-24 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain E 1–213（213 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 CDL CARDIOLIPIN × 6 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.63 Å
9TI4 High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-12-04 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain E 53–213（161 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 ZN ZINC ION × 1 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；25 mM HEPES-KOH pH=7.5, 50 mM KCl, 20 mM Mg(OAc)2, 0.01% (v/v) Lauryl Maltose Neopentyl Glycol (LMNG), 0.001 % cardiolipin, 0.001 % glycol-diosgenin (GDN, Sigma-Aldrich), 0.1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.66 Å

6 other PDB entries and 6 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUS7_HUMAN
Isoform —
PDB entities 3
Chains and sequence ranges Author chain C； PDBConstruct 1–213； UniProt 1–213

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6

OrganismNot specified

UniProt P56556

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain E； UniProt 1–128 Not recorded NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P49821） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （O00217） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （O75251） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （O43678） Acyl carrier protein, mitochondrial × 2 （O14561） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q16718） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （O95182） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q16795） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （O43181） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P28331） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q9UI09） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P19404） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （O75489） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （O75306） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O15239） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （O75380） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （O95167） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q86Y39） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9P0J0） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （O95178） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （O43676） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （O43674） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （O95139） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （O95169） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （O96000） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q9NX14） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （O43677） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （O95298） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （O43920） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03897） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03901） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03915） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03923） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （O75438） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （O95168） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9Y6M9） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03905） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03886） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P51970） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （P17568） Unknow peptide × 1 NADH-ubiquinone oxidoreductase chain 2 × 1 （P03891） SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.44 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9CWT Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5XTB Cryo-EM structure of human respiratory complex I matrix arm Deposited 2017-06-18 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain E 42–154（113 aa） Fragment：UNP RESIDUES 42-154
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5XTD Cryo-EM structure of human respiratory complex I Deposited 2017-06-19 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain E 42–154（113 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 9 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 CDL CARDIOLIPIN × 5 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
5XTH Cryo-EM structure of human respiratory supercomplex I1III2IV1 Deposited 2017-06-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain E 42–154（113 aa） Fragment：UNP RESIDUES 42-154
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 12 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 4 CDL CARDIOLIPIN × 14 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 10 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 HEA HEME-A × 2 ZN ZINC ION × 1 HEC HEME C × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
5XTI Cryo-EM architecture of human respiratory chain megacomplex-I2III2IV2 Deposited 2017-06-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 138 PDB declaration: 138-meric
Chain BE 42–154（113 aa） Fragment：UNP RESIDUES 42-154
Chain E 42–154（113 aa） Fragment：UNP RESIDUES 42-154
Not recorded SF4 IRON/SULFUR CLUSTER × 12 FMN FLAVIN MONONUCLEOTIDE × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 21 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 4 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 6 CDL CARDIOLIPIN × 19 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 14 CU COPPER (II) ION × 6 MG MAGNESIUM ION × 2 HEA HEME-A × 4 ZN ZINC ION × 2 HEC HEME C × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 17.40 Å
9I4I High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-01-24 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain F 1–128（128 aa）
Not recorded PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 CDL CARDIOLIPIN × 6 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.63 Å
9TI4 High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-12-04 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain F 11–128（118 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 ZN ZINC ION × 1 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；25 mM HEPES-KOH pH=7.5, 50 mM KCl, 20 mM Mg(OAc)2, 0.01% (v/v) Lauryl Maltose Neopentyl Glycol (LMNG), 0.001 % cardiolipin, 0.001 % glycol-diosgenin (GDN, Sigma-Aldrich), 0.1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.66 Å

6 other PDB entries and 6 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUA6_HUMAN
Isoform —
PDB entities 4
Chains and sequence ranges Author chain E； PDBConstruct 1–128； UniProt 1–128

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2

OrganismNot specified

UniProt O43678

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain F； UniProt 1–99 Not recorded NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P49821） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （O00217） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （O75251） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （P56556） Acyl carrier protein, mitochondrial × 2 （O14561） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q16718） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （O95182） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q16795） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （O43181） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P28331） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q9UI09） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P19404） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （O75489） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （O75306） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O15239） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （O75380） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （O95167） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q86Y39） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9P0J0） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （O95178） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （O43676） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （O43674） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （O95139） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （O95169） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （O96000） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q9NX14） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （O43677） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （O95298） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （O43920） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03897） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03901） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03915） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03923） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （O75438） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （O95168） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9Y6M9） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03905） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03886） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P51970） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （P17568） Unknow peptide × 1 NADH-ubiquinone oxidoreductase chain 2 × 1 （P03891） SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.44 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9CWT Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
1S3A NMR Solution Structure of Subunit B8 from Human NADH-Ubiquinone Oxidoreductase Complex I (CI-B8) Deposited 2004-01-13 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 0–98（99 aa）
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 6；300 K；Ionic strength (raw mmCIF value) 20mM phosphate, 50mM NaCl；Pressure ambient
NMR sample composition U-15N, 90% H2O, 10% D2O | 90% H2O/10% D2O
NMR sample composition U-15N, U-13C, 90% H2O, 10% D2O | 90% H2O/10% D2O
NMR sample composition U-15N, U-13C, 100% D2O | 100% D2O
Resolution not provided
5XTB Cryo-EM structure of human respiratory complex I matrix arm Deposited 2017-06-18 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain F 14–96（83 aa） Fragment：UNP RESIDUES 14-96
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5XTD Cryo-EM structure of human respiratory complex I Deposited 2017-06-19 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain F 14–96（83 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 9 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 CDL CARDIOLIPIN × 5 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
5XTH Cryo-EM structure of human respiratory supercomplex I1III2IV1 Deposited 2017-06-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain F 14–96（83 aa） Fragment：UNP RESIDUES 14-96
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 12 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 4 CDL CARDIOLIPIN × 14 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 10 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 HEA HEME-A × 2 ZN ZINC ION × 1 HEC HEME C × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
5XTI Cryo-EM architecture of human respiratory chain megacomplex-I2III2IV2 Deposited 2017-06-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 138 PDB declaration: 138-meric
Chain BF 14–96（83 aa） Fragment：UNP RESIDUES 14-96
Chain F 14–96（83 aa） Fragment：UNP RESIDUES 14-96
Not recorded SF4 IRON/SULFUR CLUSTER × 12 FMN FLAVIN MONONUCLEOTIDE × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 21 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 4 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 6 CDL CARDIOLIPIN × 19 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 14 CU COPPER (II) ION × 6 MG MAGNESIUM ION × 2 HEA HEME-A × 4 ZN ZINC ION × 2 HEC HEME C × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 17.40 Å
9I4I High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-01-24 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain G 1–99（99 aa）
Not recorded PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 CDL CARDIOLIPIN × 6 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.63 Å
9TI4 High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-12-04 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain G 14–98（85 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 ZN ZINC ION × 1 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；25 mM HEPES-KOH pH=7.5, 50 mM KCl, 20 mM Mg(OAc)2, 0.01% (v/v) Lauryl Maltose Neopentyl Glycol (LMNG), 0.001 % cardiolipin, 0.001 % glycol-diosgenin (GDN, Sigma-Aldrich), 0.1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.66 Å

7 other PDB entries and 7 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUA2_HUMAN
Isoform —
PDB entities 5
Chains and sequence ranges Author chain F； PDBConstruct 1–99； UniProt 1–99

Acyl carrier protein, mitochondrial

OrganismNot specified

UniProt O14561

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain G； UniProt 1–156 Chain X； UniProt 1–156 Not recorded NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P49821） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （O00217） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （O75251） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （P56556） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （O43678） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q16718） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （O95182） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q16795） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （O43181） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P28331） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q9UI09） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P19404） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （O75489） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （O75306） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O15239） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （O75380） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （O95167） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q86Y39） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9P0J0） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （O95178） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （O43676） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （O43674） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （O95139） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （O95169） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （O96000） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q9NX14） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （O43677） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （O95298） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （O43920） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03897） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03901） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03915） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03923） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （O75438） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （O95168） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9Y6M9） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03905） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03886） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P51970） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （P17568） Unknow peptide × 1 NADH-ubiquinone oxidoreductase chain 2 × 1 （P03891） SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.44 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9CWT Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
2DNW Solution structure of RSGI RUH-059, an ACP domain of acyl carrier protein, mitochondrial [Precursor] from human cDNA Deposited 2006-04-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 69–156（88 aa） Fragment：acyl carrier protein
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 7；298 K；Ionic strength (raw mmCIF value) 120mM；Pressure ambient
NMR sample composition 0.91mM RSGI RUH-059 U-15N, 13C; 20mM d-Tris-HCl; 1mM d-DTT; 0.02% NaN3; 10% D2O, 90% H2O | 90% H2O/10% D2O
Resolution not provided
5OOL Structure of a native assembly intermediate of the human mitochondrial ribosome with unfolded interfacial rRNA Deposited 2017-08-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 54 PDB declaration: 56-meric
Chain w 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 92 ZN ZINC ION × 3 PNS 4'-PHOSPHOPANTETHEINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.45；2 mM Synercid (Santa Cruz Biotechnology, Inc) was added to reduce preferential orientation.
cryo-EM vitrification conditions Cryogen ETHANE；Sample incubated for 30 s prior to freezing.
Resolution 3.06 Å
5OOM Structure of a native assembly intermediate of the human mitochondrial ribosome with unfolded interfacial rRNA Deposited 2017-08-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 55-meric
Chain w 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 49 ZN ZINC ION × 2 PNS 4'-PHOSPHOPANTETHEINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.45；2 mM Synercid (Santa Cruz Biotechnology, Inc) was added to reduce preferential orientation.
cryo-EM vitrification conditions Cryogen ETHANE；Sample incubated for 30 s prior to freezing.
Resolution 3.03 Å
5XTB Cryo-EM structure of human respiratory complex I matrix arm Deposited 2017-06-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain G 72–156（85 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5XTC Cryo-EM structure of human respiratory complex I transmembrane arm Deposited 2017-06-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 29 PDB declaration: 29-meric
Chain X 72–156（85 aa）
Not recorded PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 9 CDL CARDIOLIPIN × 5 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 4 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
5XTD Cryo-EM structure of human respiratory complex I Deposited 2017-06-19 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain G 72–156（85 aa）
Chain X 72–156（85 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 9 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 CDL CARDIOLIPIN × 5 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
5XTH Cryo-EM structure of human respiratory supercomplex I1III2IV1 Deposited 2017-06-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain G 72–156（85 aa） Fragment：UNP RESIDUES 72-156
Chain X 72–156（85 aa） Fragment：UNP RESIDUES 72-156
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 12 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 4 CDL CARDIOLIPIN × 14 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 10 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 HEA HEME-A × 2 ZN ZINC ION × 1 HEC HEME C × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
5XTI Cryo-EM architecture of human respiratory chain megacomplex-I2III2IV2 Deposited 2017-06-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 138 PDB declaration: 138-meric
Chain BG 72–156（85 aa） Fragment：UNP RESIDUES 72-196
Chain BX 72–156（85 aa） Fragment：UNP RESIDUES 72-196
Chain G 72–156（85 aa） Fragment：UNP RESIDUES 72-196
Chain X 72–156（85 aa） Fragment：UNP RESIDUES 72-196
Not recorded SF4 IRON/SULFUR CLUSTER × 12 FMN FLAVIN MONONUCLEOTIDE × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 21 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 4 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 6 CDL CARDIOLIPIN × 19 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 14 CU COPPER (II) ION × 6 MG MAGNESIUM ION × 2 HEA HEME-A × 4 ZN ZINC ION × 2 HEC HEME C × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 17.40 Å
6ODD Crystal structure of the human complex ACP-ISD11 Deposited 2019-03-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain A 72–156（85 aa）
Not recorded 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；The drop was a 1:1 mix of protein (in 10 mM Tris buffer, 25 mM NaCl, pH 7.5) and reservoir solution (0.1 M Tris pH 9.1, 0.1 M CaCl2, 23% tert-butanol)
Resolution 2.00 Å R-free 0.216
6ODD Crystal structure of the human complex ACP-ISD11 Deposited 2019-03-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 72–156（85 aa）
Not recorded 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 1 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；The drop was a 1:1 mix of protein (in 10 mM Tris buffer, 25 mM NaCl, pH 7.5) and reservoir solution (0.1 M Tris pH 9.1, 0.1 M CaCl2, 23% tert-butanol)
Resolution 2.00 Å R-free 0.216
7A5H Structure of the split human mitoribosomal large subunit with rescue factors mtRF-R and MTRES1 Deposited 2020-08-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 59-meric
Chain w 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 92 ZN ZINC ION × 2 PNS 4'-PHOSPHOPANTETHEINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
7A5J Structure of the split human mitoribosomal large subunit with P-and E-site mt-tRNAs Deposited 2020-08-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 59 PDB declaration: 63-meric
Chain w 1–156（156 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) MG MAGNESIUM ION × 92 ZN ZINC ION × 3 CL CHLORIDE ION × 2 NA SODIUM ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7O9K Human mitochondrial ribosome large subunit assembly intermediate with MTERF4-NSUN4, MRM2, MTG1, the MALSU module, GTPBP5 and mtEF-Tu Deposited 2021-04-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 67 PDB declaration: 69-meric
Chain w 1–156（156 aa）
Not recorded ZN ZINC ION × 2 MG MAGNESIUM ION × 93 SAM S-ADENOSYLMETHIONINE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SAH S-ADENOSYL-L-HOMOCYSTEINE × 1 PNS 4'-PHOSPHOPANTETHEINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7O9M Human mitochondrial ribosome large subunit assembly intermediate with MTERF4-NSUN4, MRM2, MTG1 and the MALSU module Deposited 2021-04-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 64 PDB declaration: 66-meric
Chain w 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 104 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ZN ZINC ION × 2 SAM S-ADENOSYLMETHIONINE × 1 PNS 4'-PHOSPHOPANTETHEINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å
7ODR State A of the human mitoribosomal large subunit assembly intermediate Deposited 2021-04-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 57-meric
Chain w 1–156（156 aa）
Not recorded PM8 S-(2-{[N-(2-HYDROXY-4-{[HYDROXY(OXIDO)PHOSPHINO]OXY}-3,3-DIMETHYLBUTANOYL)-BETA-ALANYL]AMINO}ETHYL) DECANETHIOATE × 1 SAM S-ADENOSYLMETHIONINE × 1 ZN ZINC ION × 2 MG MAGNESIUM ION × 97 K POTASSIUM ION × 2 FES FE2/S2 (INORGANIC) CLUSTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 uL of the sample was blotted for 2-6 sec before plunging
Resolution 2.90 Å
7ODS State B of the human mitoribosomal large subunit assembly intermediate Deposited 2021-04-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 58-meric
Chain w 1–156（156 aa）
Not recorded PM8 S-(2-{[N-(2-HYDROXY-4-{[HYDROXY(OXIDO)PHOSPHINO]OXY}-3,3-DIMETHYLBUTANOYL)-BETA-ALANYL]AMINO}ETHYL) DECANETHIOATE × 1 SAM S-ADENOSYLMETHIONINE × 1 ZN ZINC ION × 2 MG MAGNESIUM ION × 88 K POTASSIUM ION × 8 N ANY 5'-MONOPHOSPHATE NUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 uL of the sample was blotted for 2-6 sec before plunging
Resolution 3.10 Å
7ODT State C of the human mitoribosomal large subunit assembly intermediate Deposited 2021-04-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 58-meric
Chain w 1–156（156 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 95 PM8 S-(2-{[N-(2-HYDROXY-4-{[HYDROXY(OXIDO)PHOSPHINO]OXY}-3,3-DIMETHYLBUTANOYL)-BETA-ALANYL]AMINO}ETHYL) DECANETHIOATE × 1 SAM S-ADENOSYLMETHIONINE × 1 ZN ZINC ION × 2 K POTASSIUM ION × 10 FES FE2/S2 (INORGANIC) CLUSTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 uL of the sample was blotted for 2-6 sec before plunging
Resolution 3.10 Å
7OF0 Structure of a human mitochondrial ribosome large subunit assembly intermediate in complex with MTERF4-NSUN4 (dataset1). Deposited 2021-05-04 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 54 PDB declaration: 56-meric
Chain w 1–156（156 aa）
Not recorded ZN ZINC ION × 3 MG MAGNESIUM ION × 61 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.20 Å
7OF2 Structure of a human mitochondrial ribosome large subunit assembly intermediate in complex with GTPBP6. Deposited 2021-05-04 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 54 PDB declaration: 56-meric
Chain w 1–156（156 aa）
Not recorded ZN ZINC ION × 3 MG MAGNESIUM ION × 85 GTP GUANOSINE-5'-TRIPHOSPHATE × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
7OF3 Structure of a human mitochondrial ribosome large subunit assembly intermediate in complex with MTERF4-NSUN4 (dataset2). Deposited 2021-05-04 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 54 PDB declaration: 56-meric
Chain w 1–156（156 aa）
Not recorded ZN ZINC ION × 3 MG MAGNESIUM ION × 58 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
7OF5 Structure of a human mitochondrial ribosome large subunit assembly intermediate in complex with MTERF4-NSUN4 and GTPBP5 (dataset2). Deposited 2021-05-04 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 57-meric
Chain w 1–156（156 aa）
Not recorded ZN ZINC ION × 3 MG MAGNESIUM ION × 70 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
7OF7 Structure of a human mitochondrial ribosome large subunit assembly intermediate in complex with MTERF4-NSUN4 and GTPBP5 (dataset1). Deposited 2021-05-04 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 57-meric
Chain w 1–156（156 aa）
Not recorded ZN ZINC ION × 3 MG MAGNESIUM ION × 74 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
7OI6 Cryo-EM structure of late human 39S mitoribosome assembly intermediates, state 1 Deposited 2021-05-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 52-meric
Chain w 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 48 ZN ZINC ION × 1 PNS 4'-PHOSPHOPANTETHEINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.70 Å
7OI7 Cryo-EM structure of late human 39S mitoribosome assembly intermediates, state 2 Deposited 2021-05-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 54-meric
Chain w 1–156（156 aa）
Not recorded ZN ZINC ION × 2 MG MAGNESIUM ION × 49 PNS 4'-PHOSPHOPANTETHEINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
7OI8 Cryo-EM structure of late human 39S mitoribosome assembly intermediates, state 3A Deposited 2021-05-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 55-meric
Chain w 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 49 ZN ZINC ION × 2 PNS 4'-PHOSPHOPANTETHEINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
7OI9 Cryo-EM structure of late human 39S mitoribosome assembly intermediates, state 3B Deposited 2021-05-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 54-meric
Chain w 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 49 ZN ZINC ION × 2 PNS 4'-PHOSPHOPANTETHEINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
7OIC Cryo-EM structure of late human 39S mitoribosome assembly intermediates, state 4 Deposited 2021-05-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 58-meric
Chain w 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 76 ZN ZINC ION × 3 PNS 4'-PHOSPHOPANTETHEINE × 1 SAM S-ADENOSYLMETHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7OID Cryo-EM structure of late human 39S mitoribosome assembly intermediates, state 5A Deposited 2021-05-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 56-meric
Chain w 1–156（156 aa）
Not recorded ZN ZINC ION × 2 MG MAGNESIUM ION × 2 PNS 4'-PHOSPHOPANTETHEINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
7OIE Cryo-EM structure of late human 39S mitoribosome assembly intermediates, state 5B Deposited 2021-05-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 55-meric
Chain w 1–156（156 aa）
Not recorded ZN ZINC ION × 2 MG MAGNESIUM ION × 2 PNS 4'-PHOSPHOPANTETHEINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
7PD3 Structure of the human mitoribosomal large subunit in complex with NSUN4.MTERF4.GTPBP7 and MALSU1.L0R8F8.mt-ACP Deposited 2021-08-04 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 58 PDB declaration: 60-meric
Chain w 1–156（156 aa）
Not recorded ZN ZINC ION × 2 MG MAGNESIUM ION × 91 PNS 4'-PHOSPHOPANTETHEINE × 1 SAM S-ADENOSYLMETHIONINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
7PO4 Assembly intermediate of human mitochondrial ribosome large subunit (largely unfolded rRNA with MALSU1, L0R8F8 and ACP) Deposited 2021-09-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 58 PDB declaration: 60-meric
Chain zc 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 35 K POTASSIUM ION × 8 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 1 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.56 Å
7QH6 Cryo-EM structure of the human mtLSU assembly intermediate upon MRM2 depletion - class 1 Deposited 2021-12-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 44 PDB declaration: 46-meric
Chain w 1–156（156 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.08 Å
7QH7 Cryo-EM structure of the human mtLSU assembly intermediate upon MRM2 depletion - class 4 Deposited 2021-12-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 49-meric
Chain w 74–152（79 aa）
Not recorded MG MAGNESIUM ION × 50 ZN ZINC ION × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.89 Å
8K2B Cryo-EM structure of the human 39S mitoribosome with Tigecycline Deposited 2023-07-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 55-meric
Chain w 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 95 T1C TIGECYCLINE × 1 ZN ZINC ION × 3 PNS 4'-PHOSPHOPANTETHEINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8PK0 human mitoribosomal large subunit assembly intermediate 1 with GTPBP10-GTPBP7 Deposited 2023-06-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 58-meric
Chain w 1–156（156 aa）
Not recorded ZN ZINC ION × 1 PM8 S-(2-{[N-(2-HYDROXY-4-{[HYDROXY(OXIDO)PHOSPHINO]OXY}-3,3-DIMETHYLBUTANOYL)-BETA-ALANYL]AMINO}ETHYL) DECANETHIOATE × 1 SAM S-ADENOSYLMETHIONINE × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 MG MAGNESIUM ION × 96 K POTASSIUM ION × 10 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.03 Å
8QSJ Human mitoribosomal large subunit assembly intermediate 2 with GTPBP7 Deposited 2023-10-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 58-meric
Chain w 1–156（156 aa）
Not recorded ZN ZINC ION × 2 MG MAGNESIUM ION × 38 FES FE2/S2 (INORGANIC) CLUSTER × 1 PM8 S-(2-{[N-(2-HYDROXY-4-{[HYDROXY(OXIDO)PHOSPHINO]OXY}-3,3-DIMETHYLBUTANOYL)-BETA-ALANYL]AMINO}ETHYL) DECANETHIOATE × 1 SAM S-ADENOSYLMETHIONINE × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8QU5 mt-LSU assembly intermediate in GTPBP8 knock-out cells, state 2 Deposited 2023-10-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 55-meric
Chain w 1–156（156 aa）
Not recorded ZN ZINC ION × 2 MG MAGNESIUM ION × 48 PNS 4'-PHOSPHOPANTETHEINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.42 Å
9I4I High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-01-24 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain H 1–156（156 aa）
Chain X 1–156（156 aa）
Not recorded PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 CDL CARDIOLIPIN × 6 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.63 Å
9N50 Crosslinked Crystal Structure of Human Mitochondrial Ketosynthase, OXSM, and Crosslinker-crypto Human Mitochondrial Acyl Carrier Protein, C8aBr-mACP Deposited 2025-02-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain C 69–156（88 aa）
Not recorded A1BMZ N~3~-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-N-(2-octanamidoethyl)-beta-alaninamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；0.5 M ammonium sulfate, 1 M lithium sulfate, and 0.1 M sodium citrate pH 5.6
Resolution 2.50 Å
9N51 Crosslinked Crystal Structure of Human Mitochondrial Ketosynthase, OXSM, and Crosslinker-crypto Human Mitochondrial Acyl Carrier Protein, C8Cl-mACP Deposited 2025-02-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain C 69–156（88 aa）
Not recorded A1BMZ N~3~-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-N-(2-octanamidoethyl)-beta-alaninamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；0.5 M ammonium sulfate, 1 M lithium sulfate, and 0.1 M sodium citrate pH 5.6
Resolution 2.31 Å R-free 0.273
9N51 Crosslinked Crystal Structure of Human Mitochondrial Ketosynthase, OXSM, and Crosslinker-crypto Human Mitochondrial Acyl Carrier Protein, C8Cl-mACP Deposited 2025-02-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain F 69–156（88 aa）
Not recorded A1BMZ N~3~-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-N-(2-octanamidoethyl)-beta-alaninamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；0.5 M ammonium sulfate, 1 M lithium sulfate, and 0.1 M sodium citrate pH 5.6
Resolution 2.31 Å R-free 0.273
9PRA In Situ Structure of the Human Mitoribosome Large Subunit 39S in Complex with EF-Tu Deposited 2025-07-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 64 PDB declaration: 66-meric
Chain Af 1–156（156 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) ZN ZINC ION × 2 K POTASSIUM ION × 36 MG MAGNESIUM ION × 141 FES FE2/S2 (INORGANIC) CLUSTER × 1 SPD SPERMIDINE × 2 PUT 1,4-DIAMINOBUTANE × 1 VAL VALINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.83 Å
9TI4 High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-12-04 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain H 72–156（85 aa）
Chain X 72–156（85 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 ZN ZINC ION × 1 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；25 mM HEPES-KOH pH=7.5, 50 mM KCl, 20 mM Mg(OAc)2, 0.01% (v/v) Lauryl Maltose Neopentyl Glycol (LMNG), 0.001 % cardiolipin, 0.001 % glycol-diosgenin (GDN, Sigma-Aldrich), 0.1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.66 Å

41 other PDB entries and 43 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name ACPM_HUMAN
Isoform —
PDB entities 6
Chains and sequence ranges Author chain G； PDBConstruct 1–156； UniProt 1–156 Author chain X； PDBConstruct 1–156； UniProt 1–156

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5

OrganismNot specified

UniProt Q16718

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain H； UniProt 1–116 Not recorded NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P49821） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （O00217） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （O75251） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （P56556） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （O43678） Acyl carrier protein, mitochondrial × 2 （O14561） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （O95182） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q16795） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （O43181） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P28331） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q9UI09） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P19404） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （O75489） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （O75306） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O15239） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （O75380） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （O95167） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q86Y39） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9P0J0） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （O95178） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （O43676） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （O43674） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （O95139） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （O95169） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （O96000） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q9NX14） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （O43677） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （O95298） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （O43920） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03897） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03901） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03915） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03923） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （O75438） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （O95168） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9Y6M9） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03905） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03886） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P51970） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （P17568） Unknow peptide × 1 NADH-ubiquinone oxidoreductase chain 2 × 1 （P03891） SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.44 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9CWT Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5XTB Cryo-EM structure of human respiratory complex I matrix arm Deposited 2017-06-18 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain H 5–116（112 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5XTD Cryo-EM structure of human respiratory complex I Deposited 2017-06-19 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain H 5–116（112 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 9 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 CDL CARDIOLIPIN × 5 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
5XTH Cryo-EM structure of human respiratory supercomplex I1III2IV1 Deposited 2017-06-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain H 5–116（112 aa） Fragment：UNP RESIDUES 5-116
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 12 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 4 CDL CARDIOLIPIN × 14 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 10 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 HEA HEME-A × 2 ZN ZINC ION × 1 HEC HEME C × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
5XTI Cryo-EM architecture of human respiratory chain megacomplex-I2III2IV2 Deposited 2017-06-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 138 PDB declaration: 138-meric
Chain BH 5–116（112 aa） Fragment：UNP RESIDUES 5-116
Chain H 5–116（112 aa） Fragment：UNP RESIDUES 5-116
Not recorded SF4 IRON/SULFUR CLUSTER × 12 FMN FLAVIN MONONUCLEOTIDE × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 21 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 4 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 6 CDL CARDIOLIPIN × 19 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 14 CU COPPER (II) ION × 6 MG MAGNESIUM ION × 2 HEA HEME-A × 4 ZN ZINC ION × 2 HEC HEME C × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 17.40 Å
9I4I High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-01-24 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain I 1–116（116 aa）
Not recorded PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 CDL CARDIOLIPIN × 6 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.63 Å
9TI4 High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-12-04 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain I 5–116（112 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 ZN ZINC ION × 1 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；25 mM HEPES-KOH pH=7.5, 50 mM KCl, 20 mM Mg(OAc)2, 0.01% (v/v) Lauryl Maltose Neopentyl Glycol (LMNG), 0.001 % cardiolipin, 0.001 % glycol-diosgenin (GDN, Sigma-Aldrich), 0.1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.66 Å

6 other PDB entries and 6 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUA5_HUMAN
Isoform —
PDB entities 7
Chains and sequence ranges Author chain H； PDBConstruct 1–116； UniProt 1–116

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7

OrganismNot specified

UniProt O95182

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain I； UniProt 1–113 Not recorded NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P49821） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （O00217） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （O75251） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （P56556） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （O43678） Acyl carrier protein, mitochondrial × 2 （O14561） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q16718） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q16795） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （O43181） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P28331） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q9UI09） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P19404） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （O75489） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （O75306） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O15239） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （O75380） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （O95167） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q86Y39） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9P0J0） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （O95178） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （O43676） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （O43674） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （O95139） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （O95169） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （O96000） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q9NX14） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （O43677） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （O95298） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （O43920） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03897） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03901） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03915） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03923） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （O75438） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （O95168） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9Y6M9） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03905） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03886） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P51970） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （P17568） Unknow peptide × 1 NADH-ubiquinone oxidoreductase chain 2 × 1 （P03891） SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.44 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9CWT Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5XTB Cryo-EM structure of human respiratory complex I matrix arm Deposited 2017-06-18 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain I 4–113（110 aa） Fragment：UNP RESIDUES 4-113
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5XTD Cryo-EM structure of human respiratory complex I Deposited 2017-06-19 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain I 4–113（110 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 9 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 CDL CARDIOLIPIN × 5 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
5XTH Cryo-EM structure of human respiratory supercomplex I1III2IV1 Deposited 2017-06-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain I 4–113（110 aa） Fragment：UNP RESIDUES 4-113
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 12 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 4 CDL CARDIOLIPIN × 14 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 10 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 HEA HEME-A × 2 ZN ZINC ION × 1 HEC HEME C × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
5XTI Cryo-EM architecture of human respiratory chain megacomplex-I2III2IV2 Deposited 2017-06-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 138 PDB declaration: 138-meric
Chain BI 4–113（110 aa） Fragment：UNP RESIDUES 4-113
Chain I 4–113（110 aa） Fragment：UNP RESIDUES 4-113
Not recorded SF4 IRON/SULFUR CLUSTER × 12 FMN FLAVIN MONONUCLEOTIDE × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 21 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 4 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 6 CDL CARDIOLIPIN × 19 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 14 CU COPPER (II) ION × 6 MG MAGNESIUM ION × 2 HEA HEME-A × 4 ZN ZINC ION × 2 HEC HEME C × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 17.40 Å
9I4I High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-01-24 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain t 1–113（113 aa）
Not recorded PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 CDL CARDIOLIPIN × 6 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.63 Å
9TI4 High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-12-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain t 1–113（113 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 ZN ZINC ION × 1 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；25 mM HEPES-KOH pH=7.5, 50 mM KCl, 20 mM Mg(OAc)2, 0.01% (v/v) Lauryl Maltose Neopentyl Glycol (LMNG), 0.001 % cardiolipin, 0.001 % glycol-diosgenin (GDN, Sigma-Aldrich), 0.1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.66 Å

6 other PDB entries and 6 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUA7_HUMAN
Isoform —
PDB entities 8
Chains and sequence ranges Author chain I； PDBConstruct 1–113； UniProt 1–113

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial

OrganismNot specified

UniProt Q16795

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain J； UniProt 1–377 Not recorded NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P49821） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （O00217） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （O75251） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （P56556） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （O43678） Acyl carrier protein, mitochondrial × 2 （O14561） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q16718） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （O95182） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （O43181） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P28331） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q9UI09） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P19404） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （O75489） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （O75306） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O15239） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （O75380） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （O95167） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q86Y39） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9P0J0） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （O95178） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （O43676） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （O43674） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （O95139） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （O95169） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （O96000） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q9NX14） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （O43677） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （O95298） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （O43920） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03897） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03901） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03915） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03923） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （O75438） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （O95168） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9Y6M9） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03905） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03886） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P51970） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （P17568） Unknow peptide × 1 NADH-ubiquinone oxidoreductase chain 2 × 1 （P03891） SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.44 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9CWT Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5XTB Cryo-EM structure of human respiratory complex I matrix arm Deposited 2017-06-18 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain J 40–376（337 aa） Fragment：UNP RESIDUES 40-375
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5XTD Cryo-EM structure of human respiratory complex I Deposited 2017-06-19 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain J 40–376（337 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 9 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 CDL CARDIOLIPIN × 5 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
5XTH Cryo-EM structure of human respiratory supercomplex I1III2IV1 Deposited 2017-06-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain J 40–376（337 aa） Fragment：UNP RESIDUES 40-376
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 12 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 4 CDL CARDIOLIPIN × 14 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 10 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 HEA HEME-A × 2 ZN ZINC ION × 1 HEC HEME C × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
5XTI Cryo-EM architecture of human respiratory chain megacomplex-I2III2IV2 Deposited 2017-06-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 138 PDB declaration: 138-meric
Chain BJ 40–376（337 aa） Fragment：UNP RESIDUES 40-376
Chain J 40–376（337 aa） Fragment：UNP RESIDUES 40-376
Not recorded SF4 IRON/SULFUR CLUSTER × 12 FMN FLAVIN MONONUCLEOTIDE × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 21 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 4 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 6 CDL CARDIOLIPIN × 19 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 14 CU COPPER (II) ION × 6 MG MAGNESIUM ION × 2 HEA HEME-A × 4 ZN ZINC ION × 2 HEC HEME C × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 17.40 Å
9I4I High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-01-24 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain J 1–377（377 aa）
Not recorded PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 CDL CARDIOLIPIN × 6 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.63 Å
9TI4 High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-12-04 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain J 36–376（341 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 ZN ZINC ION × 1 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；25 mM HEPES-KOH pH=7.5, 50 mM KCl, 20 mM Mg(OAc)2, 0.01% (v/v) Lauryl Maltose Neopentyl Glycol (LMNG), 0.001 % cardiolipin, 0.001 % glycol-diosgenin (GDN, Sigma-Aldrich), 0.1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.66 Å

6 other PDB entries and 6 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUA9_HUMAN
Isoform —
PDB entities 9
Chains and sequence ranges Author chain J； PDBConstruct 1–377； UniProt 1–377

NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial

OrganismNot specified

UniProt P56181

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain K； UniProt 1–108 Not recorded NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P49821） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （O00217） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （O75251） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （P56556） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （O43678） Acyl carrier protein, mitochondrial × 2 （O14561） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q16718） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （O95182） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q16795） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （O43181） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P28331） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q9UI09） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P19404） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （O75489） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （O75306） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O15239） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （O75380） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （O95167） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q86Y39） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9P0J0） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （O95178） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （O43676） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （O43674） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （O95139） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （O95169） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （O96000） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q9NX14） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （O43677） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （O95298） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （O43920） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03897） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03901） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03915） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03923） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （O75438） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （O95168） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9Y6M9） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03905） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03886） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P51970） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （P17568） Unknow peptide × 1 NADH-ubiquinone oxidoreductase chain 2 × 1 （P03891） SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.44 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9CWT Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5XTB Cryo-EM structure of human respiratory complex I matrix arm Deposited 2017-06-18 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain K 74–106（33 aa） Fragment：UNP RESIDUES 74-106
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5XTD Cryo-EM structure of human respiratory complex I Deposited 2017-06-19 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain K 74–106（33 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 9 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 CDL CARDIOLIPIN × 5 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
5XTH Cryo-EM structure of human respiratory supercomplex I1III2IV1 Deposited 2017-06-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain K 74–106（33 aa） Fragment：UNP RESIDUES 74-106
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 12 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 4 CDL CARDIOLIPIN × 14 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 10 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 HEA HEME-A × 2 ZN ZINC ION × 1 HEC HEME C × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
5XTI Cryo-EM architecture of human respiratory chain megacomplex-I2III2IV2 Deposited 2017-06-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 138 PDB declaration: 138-meric
Chain BK 74–106（33 aa） Fragment：UNP RESIDUES 74-106
Chain K 74–106（33 aa） Fragment：UNP RESIDUES 74-106
Not recorded SF4 IRON/SULFUR CLUSTER × 12 FMN FLAVIN MONONUCLEOTIDE × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 21 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 4 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 6 CDL CARDIOLIPIN × 19 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 14 CU COPPER (II) ION × 6 MG MAGNESIUM ION × 2 HEA HEME-A × 4 ZN ZINC ION × 2 HEC HEME C × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 17.40 Å
9I4I High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-01-24 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain K 1–108（108 aa）
Not recorded PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 CDL CARDIOLIPIN × 6 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.63 Å
9TI4 High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-12-04 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain K 68–106（39 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 ZN ZINC ION × 1 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；25 mM HEPES-KOH pH=7.5, 50 mM KCl, 20 mM Mg(OAc)2, 0.01% (v/v) Lauryl Maltose Neopentyl Glycol (LMNG), 0.001 % cardiolipin, 0.001 % glycol-diosgenin (GDN, Sigma-Aldrich), 0.1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.66 Å
9W2U Cryo-EM structure of complex I on the bovine heart submitochondrial particles, open Deposited 2025-07-28 Different construct Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain s 64–108（45 aa）
Not recorded FME N-FORMYLMETHIONINE × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å
9W2V Cryo-EM structure of complex I on the bovine heart submitochondrial particles, closed Deposited 2025-07-28 Different construct Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain s 64–108（45 aa）
Not recorded FME N-FORMYLMETHIONINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å

8 other PDB entries and 8 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUV3_HUMAN
Isoform —
PDB entities 10
Chains and sequence ranges Author chain K； PDBConstruct 1–108； UniProt 1–108

NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial

OrganismNot specified

UniProt O43181

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain L； UniProt 1–175 Not recorded NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P49821） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （O00217） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （O75251） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （P56556） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （O43678） Acyl carrier protein, mitochondrial × 2 （O14561） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q16718） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （O95182） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q16795） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P28331） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q9UI09） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P19404） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （O75489） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （O75306） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O15239） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （O75380） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （O95167） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q86Y39） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9P0J0） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （O95178） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （O43676） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （O43674） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （O95139） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （O95169） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （O96000） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q9NX14） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （O43677） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （O95298） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （O43920） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03897） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03901） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03915） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03923） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （O75438） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （O95168） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9Y6M9） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03905） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03886） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P51970） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （P17568） Unknow peptide × 1 NADH-ubiquinone oxidoreductase chain 2 × 1 （P03891） SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.44 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9CWT Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5XTB Cryo-EM structure of human respiratory complex I matrix arm Deposited 2017-06-18 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain L 58–175（118 aa） Fragment：UNP RESIDUES 58-175
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5XTD Cryo-EM structure of human respiratory complex I Deposited 2017-06-19 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain L 58–175（118 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 9 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 CDL CARDIOLIPIN × 5 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
5XTH Cryo-EM structure of human respiratory supercomplex I1III2IV1 Deposited 2017-06-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain L 58–175（118 aa） Fragment：UNP RESIDUES 58-175
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 12 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 4 CDL CARDIOLIPIN × 14 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 10 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 HEA HEME-A × 2 ZN ZINC ION × 1 HEC HEME C × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
5XTI Cryo-EM architecture of human respiratory chain megacomplex-I2III2IV2 Deposited 2017-06-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 138 PDB declaration: 138-meric
Chain BL 58–175（118 aa） Fragment：UNP RESIDUES 58-175
Chain L 58–175（118 aa） Fragment：UNP RESIDUES 58-175
Not recorded SF4 IRON/SULFUR CLUSTER × 12 FMN FLAVIN MONONUCLEOTIDE × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 21 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 4 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 6 CDL CARDIOLIPIN × 19 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 14 CU COPPER (II) ION × 6 MG MAGNESIUM ION × 2 HEA HEME-A × 4 ZN ZINC ION × 2 HEC HEME C × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 17.40 Å
9I4I High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-01-24 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain L 1–175（175 aa）
Not recorded PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 CDL CARDIOLIPIN × 6 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.63 Å
9TI4 High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-12-04 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain L 55–175（121 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 ZN ZINC ION × 1 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；25 mM HEPES-KOH pH=7.5, 50 mM KCl, 20 mM Mg(OAc)2, 0.01% (v/v) Lauryl Maltose Neopentyl Glycol (LMNG), 0.001 % cardiolipin, 0.001 % glycol-diosgenin (GDN, Sigma-Aldrich), 0.1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.66 Å

6 other PDB entries and 6 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUS4_HUMAN
Isoform —
PDB entities 11
Chains and sequence ranges Author chain L； PDBConstruct 1–175； UniProt 1–175

NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial

OrganismNot specified

UniProt P28331

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain M； UniProt 1–727 Not recorded NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P49821） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （O00217） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （O75251） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （P56556） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （O43678） Acyl carrier protein, mitochondrial × 2 （O14561） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q16718） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （O95182） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q16795） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （O43181） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q9UI09） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P19404） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （O75489） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （O75306） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O15239） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （O75380） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （O95167） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q86Y39） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9P0J0） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （O95178） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （O43676） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （O43674） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （O95139） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （O95169） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （O96000） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q9NX14） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （O43677） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （O95298） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （O43920） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03897） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03901） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03915） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03923） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （O75438） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （O95168） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9Y6M9） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03905） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03886） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P51970） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （P17568） Unknow peptide × 1 NADH-ubiquinone oxidoreductase chain 2 × 1 （P03891） SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.44 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9CWT Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5XTB Cryo-EM structure of human respiratory complex I matrix arm Deposited 2017-06-18 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain M 30–716（687 aa） Fragment：UNP RESIDUES 30-716
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5XTD Cryo-EM structure of human respiratory complex I Deposited 2017-06-19 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain M 30–716（687 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 9 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 CDL CARDIOLIPIN × 5 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
5XTH Cryo-EM structure of human respiratory supercomplex I1III2IV1 Deposited 2017-06-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain M 30–716（687 aa） Fragment：UNP RESIDUES 30-716
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 12 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 4 CDL CARDIOLIPIN × 14 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 10 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 HEA HEME-A × 2 ZN ZINC ION × 1 HEC HEME C × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
5XTI Cryo-EM architecture of human respiratory chain megacomplex-I2III2IV2 Deposited 2017-06-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 138 PDB declaration: 138-meric
Chain BM 30–716（687 aa） Fragment：UNP RESIDUES 30-716
Chain M 30–716（687 aa） Fragment：UNP RESIDUES 30-716
Not recorded SF4 IRON/SULFUR CLUSTER × 12 FMN FLAVIN MONONUCLEOTIDE × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 21 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 4 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 6 CDL CARDIOLIPIN × 19 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 14 CU COPPER (II) ION × 6 MG MAGNESIUM ION × 2 HEA HEME-A × 4 ZN ZINC ION × 2 HEC HEME C × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 17.40 Å
9I4I High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-01-24 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain M 1–727（727 aa）
Not recorded PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 CDL CARDIOLIPIN × 6 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.63 Å
9TI4 High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-12-04 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain M 30–716（687 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 ZN ZINC ION × 1 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；25 mM HEPES-KOH pH=7.5, 50 mM KCl, 20 mM Mg(OAc)2, 0.01% (v/v) Lauryl Maltose Neopentyl Glycol (LMNG), 0.001 % cardiolipin, 0.001 % glycol-diosgenin (GDN, Sigma-Aldrich), 0.1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.66 Å

6 other PDB entries and 6 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUS1_HUMAN
Isoform —
PDB entities 12
Chains and sequence ranges Author chain M； PDBConstruct 1–727； UniProt 1–727

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12

OrganismNot specified

UniProt Q9UI09

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain N； UniProt 1–145 Not recorded NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P49821） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （O00217） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （O75251） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （P56556） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （O43678） Acyl carrier protein, mitochondrial × 2 （O14561） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q16718） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （O95182） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q16795） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （O43181） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P28331） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P19404） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （O75489） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （O75306） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O15239） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （O75380） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （O95167） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q86Y39） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9P0J0） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （O95178） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （O43676） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （O43674） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （O95139） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （O95169） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （O96000） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q9NX14） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （O43677） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （O95298） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （O43920） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03897） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03901） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03915） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03923） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （O75438） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （O95168） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9Y6M9） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03905） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03886） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P51970） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （P17568） Unknow peptide × 1 NADH-ubiquinone oxidoreductase chain 2 × 1 （P03891） SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.44 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9CWT Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5XTB Cryo-EM structure of human respiratory complex I matrix arm Deposited 2017-06-18 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain N 2–144（143 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5XTD Cryo-EM structure of human respiratory complex I Deposited 2017-06-19 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain N 2–144（143 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 9 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 CDL CARDIOLIPIN × 5 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
5XTH Cryo-EM structure of human respiratory supercomplex I1III2IV1 Deposited 2017-06-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain N 2–144（143 aa） Fragment：UNP RESIDUES 2-144
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 12 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 4 CDL CARDIOLIPIN × 14 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 10 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 HEA HEME-A × 2 ZN ZINC ION × 1 HEC HEME C × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
5XTI Cryo-EM architecture of human respiratory chain megacomplex-I2III2IV2 Deposited 2017-06-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 138 PDB declaration: 138-meric
Chain BN 2–144（143 aa） Fragment：UNP RESIDUES 2-144
Chain N 2–144（143 aa） Fragment：UNP RESIDUES 2-144
Not recorded SF4 IRON/SULFUR CLUSTER × 12 FMN FLAVIN MONONUCLEOTIDE × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 21 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 4 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 6 CDL CARDIOLIPIN × 19 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 14 CU COPPER (II) ION × 6 MG MAGNESIUM ION × 2 HEA HEME-A × 4 ZN ZINC ION × 2 HEC HEME C × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 17.40 Å
9I4I High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-01-24 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain N 1–145（145 aa）
Not recorded PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 CDL CARDIOLIPIN × 6 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.63 Å
9TI4 High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-12-04 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain N 1–144（144 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 ZN ZINC ION × 1 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；25 mM HEPES-KOH pH=7.5, 50 mM KCl, 20 mM Mg(OAc)2, 0.01% (v/v) Lauryl Maltose Neopentyl Glycol (LMNG), 0.001 % cardiolipin, 0.001 % glycol-diosgenin (GDN, Sigma-Aldrich), 0.1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.66 Å

6 other PDB entries and 6 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUAC_HUMAN
Isoform —
PDB entities 13
Chains and sequence ranges Author chain N； PDBConstruct 1–145； UniProt 1–145

NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial

OrganismNot specified

UniProt P19404

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain O； UniProt 1–249 Not recorded NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P49821） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （O00217） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （O75251） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （P56556） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （O43678） Acyl carrier protein, mitochondrial × 2 （O14561） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q16718） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （O95182） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q16795） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （O43181） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P28331） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q9UI09） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （O75489） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （O75306） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O15239） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （O75380） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （O95167） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q86Y39） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9P0J0） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （O95178） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （O43676） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （O43674） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （O95139） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （O95169） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （O96000） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q9NX14） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （O43677） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （O95298） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （O43920） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03897） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03901） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03915） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03923） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （O75438） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （O95168） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9Y6M9） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03905） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03886） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P51970） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （P17568） Unknow peptide × 1 NADH-ubiquinone oxidoreductase chain 2 × 1 （P03891） SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.44 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9CWT Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5XTB Cryo-EM structure of human respiratory complex I matrix arm Deposited 2017-06-18 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain O 36–247（212 aa） Fragment：UNP RESIDUES 36-247
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5XTD Cryo-EM structure of human respiratory complex I Deposited 2017-06-19 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain O 36–247（212 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 9 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 CDL CARDIOLIPIN × 5 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
5XTH Cryo-EM structure of human respiratory supercomplex I1III2IV1 Deposited 2017-06-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain O 36–247（212 aa） Fragment：UNP RESIDUES 36-247
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 12 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 4 CDL CARDIOLIPIN × 14 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 10 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 HEA HEME-A × 2 ZN ZINC ION × 1 HEC HEME C × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
5XTI Cryo-EM architecture of human respiratory chain megacomplex-I2III2IV2 Deposited 2017-06-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 138 PDB declaration: 138-meric
Chain BO 36–247（212 aa） Fragment：UNP RESIDUES 36-247
Chain O 36–247（212 aa） Fragment：UNP RESIDUES 36-247
Not recorded SF4 IRON/SULFUR CLUSTER × 12 FMN FLAVIN MONONUCLEOTIDE × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 21 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 4 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 6 CDL CARDIOLIPIN × 19 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 14 CU COPPER (II) ION × 6 MG MAGNESIUM ION × 2 HEA HEME-A × 4 ZN ZINC ION × 2 HEC HEME C × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 17.40 Å
9I4I High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-01-24 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain O 1–249（249 aa）
Not recorded PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 CDL CARDIOLIPIN × 6 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.63 Å
9TI4 High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-12-04 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain O 36–247（212 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 ZN ZINC ION × 1 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；25 mM HEPES-KOH pH=7.5, 50 mM KCl, 20 mM Mg(OAc)2, 0.01% (v/v) Lauryl Maltose Neopentyl Glycol (LMNG), 0.001 % cardiolipin, 0.001 % glycol-diosgenin (GDN, Sigma-Aldrich), 0.1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.66 Å

6 other PDB entries and 6 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUV2_HUMAN
Isoform —
PDB entities 14
Chains and sequence ranges Author chain O； PDBConstruct 1–249； UniProt 1–249

NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial

OrganismNot specified

UniProt O75489

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain P； UniProt 1–264 Not recorded NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P49821） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （O00217） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （O75251） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （P56556） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （O43678） Acyl carrier protein, mitochondrial × 2 （O14561） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q16718） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （O95182） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q16795） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （O43181） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P28331） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q9UI09） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P19404） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （O75306） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O15239） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （O75380） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （O95167） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q86Y39） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9P0J0） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （O95178） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （O43676） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （O43674） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （O95139） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （O95169） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （O96000） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q9NX14） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （O43677） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （O95298） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （O43920） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03897） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03901） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03915） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03923） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （O75438） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （O95168） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9Y6M9） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03905） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03886） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P51970） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （P17568） Unknow peptide × 1 NADH-ubiquinone oxidoreductase chain 2 × 1 （P03891） SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.44 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9CWT Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5XTB Cryo-EM structure of human respiratory complex I matrix arm Deposited 2017-06-18 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain P 43–250（208 aa） Fragment：UNP RESIDUES 43-250
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5XTD Cryo-EM structure of human respiratory complex I Deposited 2017-06-19 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain P 43–250（208 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 9 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 CDL CARDIOLIPIN × 5 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
5XTH Cryo-EM structure of human respiratory supercomplex I1III2IV1 Deposited 2017-06-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain P 43–250（208 aa） Fragment：UNP RESIDUES 43-250
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 12 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 4 CDL CARDIOLIPIN × 14 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 10 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 HEA HEME-A × 2 ZN ZINC ION × 1 HEC HEME C × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
5XTI Cryo-EM architecture of human respiratory chain megacomplex-I2III2IV2 Deposited 2017-06-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 138 PDB declaration: 138-meric
Chain BP 43–250（208 aa） Fragment：UNP RESIDUES 43-250
Chain P 43–250（208 aa） Fragment：UNP RESIDUES 43-250
Not recorded SF4 IRON/SULFUR CLUSTER × 12 FMN FLAVIN MONONUCLEOTIDE × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 21 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 4 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 6 CDL CARDIOLIPIN × 19 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 14 CU COPPER (II) ION × 6 MG MAGNESIUM ION × 2 HEA HEME-A × 4 ZN ZINC ION × 2 HEC HEME C × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 17.40 Å
9I4I High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-01-24 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain P 1–264（264 aa）
Not recorded PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 CDL CARDIOLIPIN × 6 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.63 Å
9TI4 High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-12-04 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain P 43–250（208 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 ZN ZINC ION × 1 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；25 mM HEPES-KOH pH=7.5, 50 mM KCl, 20 mM Mg(OAc)2, 0.01% (v/v) Lauryl Maltose Neopentyl Glycol (LMNG), 0.001 % cardiolipin, 0.001 % glycol-diosgenin (GDN, Sigma-Aldrich), 0.1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.66 Å

6 other PDB entries and 6 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUS3_HUMAN
Isoform —
PDB entities 15
Chains and sequence ranges Author chain P； PDBConstruct 1–264； UniProt 1–264

NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial

OrganismNot specified

UniProt O75306

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain Q； UniProt 1–463 Not recorded NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P49821） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （O00217） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （O75251） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （P56556） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （O43678） Acyl carrier protein, mitochondrial × 2 （O14561） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q16718） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （O95182） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q16795） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （O43181） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P28331） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q9UI09） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P19404） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （O75489） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O15239） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （O75380） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （O95167） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q86Y39） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9P0J0） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （O95178） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （O43676） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （O43674） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （O95139） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （O95169） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （O96000） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q9NX14） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （O43677） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （O95298） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （O43920） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03897） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03901） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03915） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03923） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （O75438） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （O95168） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9Y6M9） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03905） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03886） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P51970） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （P17568） Unknow peptide × 1 NADH-ubiquinone oxidoreductase chain 2 × 1 （P03891） SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.44 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9CWT Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5XTB Cryo-EM structure of human respiratory complex I matrix arm Deposited 2017-06-18 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain Q 79–463（385 aa） Fragment：UNP RESIDUES 79-463
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5XTC Cryo-EM structure of human respiratory complex I transmembrane arm Deposited 2017-06-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 29 PDB declaration: 29-meric
Chain Q 34–79（46 aa） Fragment：UNP RESIDUES 34-79
Not recorded PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 9 CDL CARDIOLIPIN × 5 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 4 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
5XTD Cryo-EM structure of human respiratory complex I Deposited 2017-06-19 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Q 34–463（430 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 9 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 CDL CARDIOLIPIN × 5 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
5XTH Cryo-EM structure of human respiratory supercomplex I1III2IV1 Deposited 2017-06-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain Q 34–463（430 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 12 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 4 CDL CARDIOLIPIN × 14 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 10 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 HEA HEME-A × 2 ZN ZINC ION × 1 HEC HEME C × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
5XTI Cryo-EM architecture of human respiratory chain megacomplex-I2III2IV2 Deposited 2017-06-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 138 PDB declaration: 138-meric
Chain BQ 34–463（430 aa）
Chain Q 34–463（430 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 12 FMN FLAVIN MONONUCLEOTIDE × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 21 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 4 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 6 CDL CARDIOLIPIN × 19 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 14 CU COPPER (II) ION × 6 MG MAGNESIUM ION × 2 HEA HEME-A × 4 ZN ZINC ION × 2 HEC HEME C × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 17.40 Å
9I4I High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-01-24 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Q 1–463（463 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 CDL CARDIOLIPIN × 6 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.63 Å
9TI4 High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-12-04 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Q 34–463（430 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 ZN ZINC ION × 1 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；25 mM HEPES-KOH pH=7.5, 50 mM KCl, 20 mM Mg(OAc)2, 0.01% (v/v) Lauryl Maltose Neopentyl Glycol (LMNG), 0.001 % cardiolipin, 0.001 % glycol-diosgenin (GDN, Sigma-Aldrich), 0.1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.66 Å

7 other PDB entries and 7 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUS2_HUMAN
Isoform —
PDB entities 16
Chains and sequence ranges Author chain Q； PDBConstruct 1–463； UniProt 1–463

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1

OrganismNot specified

UniProt O15239

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain S； UniProt 1–70 Not recorded NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P49821） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （O00217） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （O75251） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （P56556） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （O43678） Acyl carrier protein, mitochondrial × 2 （O14561） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q16718） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （O95182） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q16795） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （O43181） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P28331） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q9UI09） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P19404） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （O75489） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （O75306） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （O75380） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （O95167） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q86Y39） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9P0J0） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （O95178） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （O43676） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （O43674） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （O95139） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （O95169） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （O96000） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q9NX14） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （O43677） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （O95298） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （O43920） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03897） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03901） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03915） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03923） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （O75438） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （O95168） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9Y6M9） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03905） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03886） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P51970） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （P17568） Unknow peptide × 1 NADH-ubiquinone oxidoreductase chain 2 × 1 （P03891） SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.44 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9CWT Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5XTC Cryo-EM structure of human respiratory complex I transmembrane arm Deposited 2017-06-19 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 29 PDB declaration: 29-meric
Chain S 1–70（70 aa）
Not recorded PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 9 CDL CARDIOLIPIN × 5 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 4 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
5XTD Cryo-EM structure of human respiratory complex I Deposited 2017-06-19 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain S 1–70（70 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 9 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 CDL CARDIOLIPIN × 5 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
5XTH Cryo-EM structure of human respiratory supercomplex I1III2IV1 Deposited 2017-06-19 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain S 1–70（70 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 12 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 4 CDL CARDIOLIPIN × 14 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 10 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 HEA HEME-A × 2 ZN ZINC ION × 1 HEC HEME C × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
5XTI Cryo-EM architecture of human respiratory chain megacomplex-I2III2IV2 Deposited 2017-06-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 138 PDB declaration: 138-meric
Chain BS 1–70（70 aa）
Chain S 1–70（70 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 12 FMN FLAVIN MONONUCLEOTIDE × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 21 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 4 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 6 CDL CARDIOLIPIN × 19 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 14 CU COPPER (II) ION × 6 MG MAGNESIUM ION × 2 HEA HEME-A × 4 ZN ZINC ION × 2 HEC HEME C × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 17.40 Å
9I4I High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-01-24 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain S 1–70（70 aa）
Not recorded PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 CDL CARDIOLIPIN × 6 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.63 Å
9TI4 High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-12-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain S 1–70（70 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 ZN ZINC ION × 1 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；25 mM HEPES-KOH pH=7.5, 50 mM KCl, 20 mM Mg(OAc)2, 0.01% (v/v) Lauryl Maltose Neopentyl Glycol (LMNG), 0.001 % cardiolipin, 0.001 % glycol-diosgenin (GDN, Sigma-Aldrich), 0.1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.66 Å

6 other PDB entries and 6 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUA1_HUMAN
Isoform —
PDB entities 17
Chains and sequence ranges Author chain S； PDBConstruct 1–70； UniProt 1–70

NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial

OrganismNot specified

UniProt O75380

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain T； UniProt 1–124 Not recorded NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P49821） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （O00217） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （O75251） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （P56556） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （O43678） Acyl carrier protein, mitochondrial × 2 （O14561） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q16718） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （O95182） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q16795） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （O43181） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P28331） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q9UI09） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P19404） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （O75489） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （O75306） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O15239） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （O95167） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q86Y39） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9P0J0） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （O95178） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （O43676） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （O43674） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （O95139） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （O95169） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （O96000） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q9NX14） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （O43677） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （O95298） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （O43920） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03897） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03901） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03915） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03923） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （O75438） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （O95168） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9Y6M9） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03905） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03886） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P51970） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （P17568） Unknow peptide × 1 NADH-ubiquinone oxidoreductase chain 2 × 1 （P03891） SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.44 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9CWT Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5XTB Cryo-EM structure of human respiratory complex I matrix arm Deposited 2017-06-18 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain T 29–123（95 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5XTD Cryo-EM structure of human respiratory complex I Deposited 2017-06-19 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 29–123（95 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 9 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 CDL CARDIOLIPIN × 5 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
5XTH Cryo-EM structure of human respiratory supercomplex I1III2IV1 Deposited 2017-06-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain T 29–123（95 aa） Fragment：UNP RESIDUES 29-123
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 12 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 4 CDL CARDIOLIPIN × 14 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 10 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 HEA HEME-A × 2 ZN ZINC ION × 1 HEC HEME C × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
5XTI Cryo-EM architecture of human respiratory chain megacomplex-I2III2IV2 Deposited 2017-06-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 138 PDB declaration: 138-meric
Chain BT 29–123（95 aa） Fragment：UNP RESIDUES 29-123
Chain T 29–123（95 aa） Fragment：UNP RESIDUES 29-123
Not recorded SF4 IRON/SULFUR CLUSTER × 12 FMN FLAVIN MONONUCLEOTIDE × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 21 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 4 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 6 CDL CARDIOLIPIN × 19 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 14 CU COPPER (II) ION × 6 MG MAGNESIUM ION × 2 HEA HEME-A × 4 ZN ZINC ION × 2 HEC HEME C × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 17.40 Å
9I4I High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-01-24 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 1–124（124 aa）
Not recorded PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 CDL CARDIOLIPIN × 6 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.63 Å
9TI4 High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-12-04 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 29–124（96 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 ZN ZINC ION × 1 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；25 mM HEPES-KOH pH=7.5, 50 mM KCl, 20 mM Mg(OAc)2, 0.01% (v/v) Lauryl Maltose Neopentyl Glycol (LMNG), 0.001 % cardiolipin, 0.001 % glycol-diosgenin (GDN, Sigma-Aldrich), 0.1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.66 Å

6 other PDB entries and 6 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUS6_HUMAN
Isoform —
PDB entities 18
Chains and sequence ranges Author chain T； PDBConstruct 1–124； UniProt 1–124

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3

OrganismNot specified

UniProt O95167

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain U； UniProt 1–84 Not recorded NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P49821） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （O00217） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （O75251） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （P56556） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （O43678） Acyl carrier protein, mitochondrial × 2 （O14561） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q16718） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （O95182） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q16795） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （O43181） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P28331） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q9UI09） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P19404） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （O75489） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （O75306） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O15239） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （O75380） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q86Y39） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9P0J0） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （O95178） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （O43676） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （O43674） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （O95139） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （O95169） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （O96000） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q9NX14） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （O43677） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （O95298） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （O43920） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03897） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03901） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03915） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03923） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （O75438） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （O95168） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9Y6M9） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03905） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03886） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P51970） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （P17568） Unknow peptide × 1 NADH-ubiquinone oxidoreductase chain 2 × 1 （P03891） SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.44 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9CWT Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5XTC Cryo-EM structure of human respiratory complex I transmembrane arm Deposited 2017-06-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 29 PDB declaration: 29-meric
Chain U 2–84（83 aa）
Not recorded PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 9 CDL CARDIOLIPIN × 5 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 4 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
5XTD Cryo-EM structure of human respiratory complex I Deposited 2017-06-19 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain U 2–84（83 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 9 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 CDL CARDIOLIPIN × 5 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
5XTH Cryo-EM structure of human respiratory supercomplex I1III2IV1 Deposited 2017-06-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain U 2–84（83 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 12 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 4 CDL CARDIOLIPIN × 14 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 10 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 HEA HEME-A × 2 ZN ZINC ION × 1 HEC HEME C × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
5XTI Cryo-EM architecture of human respiratory chain megacomplex-I2III2IV2 Deposited 2017-06-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 138 PDB declaration: 138-meric
Chain BU 2–84（83 aa）
Chain U 2–84（83 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 12 FMN FLAVIN MONONUCLEOTIDE × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 21 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 4 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 6 CDL CARDIOLIPIN × 19 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 14 CU COPPER (II) ION × 6 MG MAGNESIUM ION × 2 HEA HEME-A × 4 ZN ZINC ION × 2 HEC HEME C × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 17.40 Å
9I4I High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-01-24 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain U 1–84（84 aa）
Not recorded PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 CDL CARDIOLIPIN × 6 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.63 Å
9TI4 High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-12-04 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain U 2–84（83 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 ZN ZINC ION × 1 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；25 mM HEPES-KOH pH=7.5, 50 mM KCl, 20 mM Mg(OAc)2, 0.01% (v/v) Lauryl Maltose Neopentyl Glycol (LMNG), 0.001 % cardiolipin, 0.001 % glycol-diosgenin (GDN, Sigma-Aldrich), 0.1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.66 Å

6 other PDB entries and 6 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUA3_HUMAN
Isoform —
PDB entities 19
Chains and sequence ranges Author chain U； PDBConstruct 1–84； UniProt 1–84

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11

OrganismNot specified

UniProt Q86Y39

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain V； UniProt 1–141 Not recorded NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P49821） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （O00217） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （O75251） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （P56556） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （O43678） Acyl carrier protein, mitochondrial × 2 （O14561） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q16718） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （O95182） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q16795） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （O43181） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P28331） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q9UI09） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P19404） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （O75489） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （O75306） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O15239） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （O75380） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （O95167） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9P0J0） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （O95178） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （O43676） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （O43674） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （O95139） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （O95169） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （O96000） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q9NX14） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （O43677） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （O95298） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （O43920） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03897） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03901） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03915） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03923） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （O75438） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （O95168） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9Y6M9） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03905） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03886） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P51970） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （P17568） Unknow peptide × 1 NADH-ubiquinone oxidoreductase chain 2 × 1 （P03891） SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.44 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9CWT Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5XTC Cryo-EM structure of human respiratory complex I transmembrane arm Deposited 2017-06-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 29 PDB declaration: 29-meric
Chain V 2–141（140 aa）
Not recorded PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 9 CDL CARDIOLIPIN × 5 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 4 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
5XTD Cryo-EM structure of human respiratory complex I Deposited 2017-06-19 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain V 2–141（140 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 9 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 CDL CARDIOLIPIN × 5 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
5XTH Cryo-EM structure of human respiratory supercomplex I1III2IV1 Deposited 2017-06-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain V 2–141（140 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 12 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 4 CDL CARDIOLIPIN × 14 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 10 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 HEA HEME-A × 2 ZN ZINC ION × 1 HEC HEME C × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
5XTI Cryo-EM architecture of human respiratory chain megacomplex-I2III2IV2 Deposited 2017-06-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 138 PDB declaration: 138-meric
Chain BV 2–141（140 aa）
Chain V 2–141（140 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 12 FMN FLAVIN MONONUCLEOTIDE × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 21 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 4 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 6 CDL CARDIOLIPIN × 19 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 14 CU COPPER (II) ION × 6 MG MAGNESIUM ION × 2 HEA HEME-A × 4 ZN ZINC ION × 2 HEC HEME C × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 17.40 Å
9I4I High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-01-24 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain V 1–141（141 aa）
Not recorded PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 CDL CARDIOLIPIN × 6 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.63 Å
9TI4 High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-12-04 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain V 2–141（140 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 ZN ZINC ION × 1 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；25 mM HEPES-KOH pH=7.5, 50 mM KCl, 20 mM Mg(OAc)2, 0.01% (v/v) Lauryl Maltose Neopentyl Glycol (LMNG), 0.001 % cardiolipin, 0.001 % glycol-diosgenin (GDN, Sigma-Aldrich), 0.1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.66 Å

6 other PDB entries and 6 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUAB_HUMAN
Isoform —
PDB entities 20
Chains and sequence ranges Author chain V； PDBConstruct 1–141； UniProt 1–141

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13

OrganismNot specified

UniProt Q9P0J0

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain W； UniProt 1–144 Not recorded NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P49821） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （O00217） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （O75251） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （P56556） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （O43678） Acyl carrier protein, mitochondrial × 2 （O14561） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q16718） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （O95182） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q16795） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （O43181） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P28331） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q9UI09） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P19404） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （O75489） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （O75306） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O15239） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （O75380） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （O95167） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q86Y39） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （O95178） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （O43676） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （O43674） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （O95139） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （O95169） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （O96000） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q9NX14） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （O43677） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （O95298） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （O43920） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03897） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03901） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03915） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03923） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （O75438） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （O95168） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9Y6M9） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03905） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03886） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P51970） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （P17568） Unknow peptide × 1 NADH-ubiquinone oxidoreductase chain 2 × 1 （P03891） SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.44 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9CWT Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5XTB Cryo-EM structure of human respiratory complex I matrix arm Deposited 2017-06-18 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain W 7–28（22 aa） Fragment：UNP RESIDUES 7-28
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5XTC Cryo-EM structure of human respiratory complex I transmembrane arm Deposited 2017-06-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 29 PDB declaration: 29-meric
Chain W 29–144（116 aa） Fragment：UNP RESIDUES 29-144
Not recorded PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 9 CDL CARDIOLIPIN × 5 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 4 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
5XTD Cryo-EM structure of human respiratory complex I Deposited 2017-06-19 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain W 7–144（138 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 9 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 CDL CARDIOLIPIN × 5 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
5XTH Cryo-EM structure of human respiratory supercomplex I1III2IV1 Deposited 2017-06-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain W 7–144（138 aa） Fragment：UNP RESIDUES 7-144
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 12 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 4 CDL CARDIOLIPIN × 14 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 10 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 HEA HEME-A × 2 ZN ZINC ION × 1 HEC HEME C × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
5XTI Cryo-EM architecture of human respiratory chain megacomplex-I2III2IV2 Deposited 2017-06-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 138 PDB declaration: 138-meric
Chain BW 7–144（138 aa） Fragment：UNP RESIDUES 7-144
Chain W 7–144（138 aa） Fragment：UNP RESIDUES 7-144
Not recorded SF4 IRON/SULFUR CLUSTER × 12 FMN FLAVIN MONONUCLEOTIDE × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 21 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 4 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 6 CDL CARDIOLIPIN × 19 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 14 CU COPPER (II) ION × 6 MG MAGNESIUM ION × 2 HEA HEME-A × 4 ZN ZINC ION × 2 HEC HEME C × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 17.40 Å
9I4I High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-01-24 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain W 1–144（144 aa）
Not recorded PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 CDL CARDIOLIPIN × 6 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.63 Å
9TI4 High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-12-04 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain W 5–144（140 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 ZN ZINC ION × 1 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；25 mM HEPES-KOH pH=7.5, 50 mM KCl, 20 mM Mg(OAc)2, 0.01% (v/v) Lauryl Maltose Neopentyl Glycol (LMNG), 0.001 % cardiolipin, 0.001 % glycol-diosgenin (GDN, Sigma-Aldrich), 0.1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.66 Å

7 other PDB entries and 7 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUAD_HUMAN
Isoform —
PDB entities 21
Chains and sequence ranges Author chain W； PDBConstruct 1–144； UniProt 1–144

NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial

OrganismNot specified

UniProt O95178

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain Y； UniProt 1–105 Not recorded NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P49821） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （O00217） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （O75251） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （P56556） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （O43678） Acyl carrier protein, mitochondrial × 2 （O14561） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q16718） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （O95182） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q16795） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （O43181） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P28331） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q9UI09） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P19404） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （O75489） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （O75306） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O15239） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （O75380） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （O95167） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q86Y39） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9P0J0） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （O43676） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （O43674） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （O95139） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （O95169） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （O96000） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q9NX14） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （O43677） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （O95298） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （O43920） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03897） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03901） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03915） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03923） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （O75438） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （O95168） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9Y6M9） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03905） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03886） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P51970） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （P17568） Unknow peptide × 1 NADH-ubiquinone oxidoreductase chain 2 × 1 （P03891） SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.44 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9CWT Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5XTC Cryo-EM structure of human respiratory complex I transmembrane arm Deposited 2017-06-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 29 PDB declaration: 29-meric
Chain Y 39–97（59 aa） Fragment：UNP RESIDUES 39-97
Not recorded PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 9 CDL CARDIOLIPIN × 5 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 4 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
5XTD Cryo-EM structure of human respiratory complex I Deposited 2017-06-19 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Y 39–97（59 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 9 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 CDL CARDIOLIPIN × 5 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
5XTH Cryo-EM structure of human respiratory supercomplex I1III2IV1 Deposited 2017-06-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain Y 39–97（59 aa） Fragment：UNP RESIDUES 39-97
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 12 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 4 CDL CARDIOLIPIN × 14 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 10 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 HEA HEME-A × 2 ZN ZINC ION × 1 HEC HEME C × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
5XTI Cryo-EM architecture of human respiratory chain megacomplex-I2III2IV2 Deposited 2017-06-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 138 PDB declaration: 138-meric
Chain BY 39–97（59 aa） Fragment：UNP RESIDUES 39-97
Chain Y 39–97（59 aa） Fragment：UNP RESIDUES 39-97
Not recorded SF4 IRON/SULFUR CLUSTER × 12 FMN FLAVIN MONONUCLEOTIDE × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 21 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 4 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 6 CDL CARDIOLIPIN × 19 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 14 CU COPPER (II) ION × 6 MG MAGNESIUM ION × 2 HEA HEME-A × 4 ZN ZINC ION × 2 HEC HEME C × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 17.40 Å
9I4I High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-01-24 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Y 1–105（105 aa）
Not recorded PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 CDL CARDIOLIPIN × 6 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.63 Å
9TI4 High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-12-04 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Y 37–97（61 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 ZN ZINC ION × 1 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；25 mM HEPES-KOH pH=7.5, 50 mM KCl, 20 mM Mg(OAc)2, 0.01% (v/v) Lauryl Maltose Neopentyl Glycol (LMNG), 0.001 % cardiolipin, 0.001 % glycol-diosgenin (GDN, Sigma-Aldrich), 0.1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.66 Å

6 other PDB entries and 6 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUB2_HUMAN
Isoform —
PDB entities 22
Chains and sequence ranges Author chain Y； PDBConstruct 1–105； UniProt 1–105

NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3

OrganismNot specified

UniProt O43676

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain Z； UniProt 1–98 Not recorded NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P49821） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （O00217） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （O75251） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （P56556） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （O43678） Acyl carrier protein, mitochondrial × 2 （O14561） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q16718） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （O95182） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q16795） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （O43181） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P28331） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q9UI09） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P19404） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （O75489） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （O75306） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O15239） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （O75380） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （O95167） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q86Y39） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9P0J0） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （O95178） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （O43674） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （O95139） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （O95169） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （O96000） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q9NX14） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （O43677） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （O95298） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （O43920） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03897） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03901） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03915） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03923） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （O75438） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （O95168） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9Y6M9） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03905） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03886） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P51970） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （P17568） Unknow peptide × 1 NADH-ubiquinone oxidoreductase chain 2 × 1 （P03891） SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.44 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9CWT Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5XTC Cryo-EM structure of human respiratory complex I transmembrane arm Deposited 2017-06-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 29 PDB declaration: 29-meric
Chain Z 10–89（80 aa） Fragment：UNP RESIDUES 10-89
Not recorded PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 9 CDL CARDIOLIPIN × 5 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 4 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
5XTD Cryo-EM structure of human respiratory complex I Deposited 2017-06-19 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Z 10–89（80 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 9 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 CDL CARDIOLIPIN × 5 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
5XTH Cryo-EM structure of human respiratory supercomplex I1III2IV1 Deposited 2017-06-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain Z 10–89（80 aa） Fragment：UNP RESIDUES 10-89
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 12 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 4 CDL CARDIOLIPIN × 14 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 10 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 HEA HEME-A × 2 ZN ZINC ION × 1 HEC HEME C × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
5XTI Cryo-EM architecture of human respiratory chain megacomplex-I2III2IV2 Deposited 2017-06-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 138 PDB declaration: 138-meric
Chain BZ 10–89（80 aa） Fragment：UNP RESIDUES 10-89
Chain Z 10–89（80 aa） Fragment：UNP RESIDUES 10-89
Not recorded SF4 IRON/SULFUR CLUSTER × 12 FMN FLAVIN MONONUCLEOTIDE × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 21 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 4 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 6 CDL CARDIOLIPIN × 19 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 14 CU COPPER (II) ION × 6 MG MAGNESIUM ION × 2 HEA HEME-A × 4 ZN ZINC ION × 2 HEC HEME C × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 17.40 Å
9I4I High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-01-24 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Z 1–98（98 aa）
Not recorded PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 CDL CARDIOLIPIN × 6 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.63 Å
9TI4 High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-12-04 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Z 10–92（83 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 ZN ZINC ION × 1 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；25 mM HEPES-KOH pH=7.5, 50 mM KCl, 20 mM Mg(OAc)2, 0.01% (v/v) Lauryl Maltose Neopentyl Glycol (LMNG), 0.001 % cardiolipin, 0.001 % glycol-diosgenin (GDN, Sigma-Aldrich), 0.1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.66 Å

6 other PDB entries and 6 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUB3_HUMAN
Isoform —
PDB entities 23
Chains and sequence ranges Author chain Z； PDBConstruct 1–98； UniProt 1–98

NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial

OrganismNot specified

UniProt O43674

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain a； UniProt 1–189 Not recorded NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P49821） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （O00217） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （O75251） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （P56556） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （O43678） Acyl carrier protein, mitochondrial × 2 （O14561） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q16718） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （O95182） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q16795） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （O43181） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P28331） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q9UI09） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P19404） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （O75489） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （O75306） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O15239） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （O75380） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （O95167） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q86Y39） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9P0J0） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （O95178） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （O43676） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （O95139） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （O95169） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （O96000） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q9NX14） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （O43677） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （O95298） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （O43920） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03897） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03901） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03915） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03923） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （O75438） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （O95168） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9Y6M9） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03905） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03886） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P51970） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （P17568） Unknow peptide × 1 NADH-ubiquinone oxidoreductase chain 2 × 1 （P03891） SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.44 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9CWT Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5XTC Cryo-EM structure of human respiratory complex I transmembrane arm Deposited 2017-06-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 29 PDB declaration: 29-meric
Chain a 52–189（138 aa）
Not recorded PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 9 CDL CARDIOLIPIN × 5 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 4 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
5XTD Cryo-EM structure of human respiratory complex I Deposited 2017-06-19 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain a 52–189（138 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 9 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 CDL CARDIOLIPIN × 5 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
5XTH Cryo-EM structure of human respiratory supercomplex I1III2IV1 Deposited 2017-06-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain a 52–189（138 aa） Fragment：UNP RESIDUES 52-189
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 12 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 4 CDL CARDIOLIPIN × 14 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 10 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 HEA HEME-A × 2 ZN ZINC ION × 1 HEC HEME C × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
5XTI Cryo-EM architecture of human respiratory chain megacomplex-I2III2IV2 Deposited 2017-06-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 138 PDB declaration: 138-meric
Chain Ba 52–189（138 aa） Fragment：UNP RESIDUES 52-189
Chain a 52–189（138 aa） Fragment：UNP RESIDUES 52-189
Not recorded SF4 IRON/SULFUR CLUSTER × 12 FMN FLAVIN MONONUCLEOTIDE × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 21 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 4 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 6 CDL CARDIOLIPIN × 19 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 14 CU COPPER (II) ION × 6 MG MAGNESIUM ION × 2 HEA HEME-A × 4 ZN ZINC ION × 2 HEC HEME C × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 17.40 Å
9I4I High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-01-24 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain a 1–189（189 aa）
Not recorded PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 CDL CARDIOLIPIN × 6 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.63 Å
9TI4 High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-12-04 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain a 52–189（138 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 ZN ZINC ION × 1 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；25 mM HEPES-KOH pH=7.5, 50 mM KCl, 20 mM Mg(OAc)2, 0.01% (v/v) Lauryl Maltose Neopentyl Glycol (LMNG), 0.001 % cardiolipin, 0.001 % glycol-diosgenin (GDN, Sigma-Aldrich), 0.1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.66 Å

6 other PDB entries and 6 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUB5_HUMAN
Isoform —
PDB entities 24
Chains and sequence ranges Author chain a； PDBConstruct 1–189； UniProt 1–189

NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6

OrganismNot specified

UniProt O95139

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain b； UniProt 1–128 Not recorded NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P49821） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （O00217） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （O75251） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （P56556） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （O43678） Acyl carrier protein, mitochondrial × 2 （O14561） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q16718） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （O95182） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q16795） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （O43181） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P28331） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q9UI09） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P19404） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （O75489） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （O75306） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O15239） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （O75380） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （O95167） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q86Y39） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9P0J0） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （O95178） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （O43676） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （O43674） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （O95169） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （O96000） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q9NX14） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （O43677） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （O95298） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （O43920） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03897） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03901） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03915） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03923） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （O75438） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （O95168） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9Y6M9） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03905） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03886） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P51970） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （P17568） Unknow peptide × 1 NADH-ubiquinone oxidoreductase chain 2 × 1 （P03891） SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.44 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9CWT Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5XTC Cryo-EM structure of human respiratory complex I transmembrane arm Deposited 2017-06-19 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 29 PDB declaration: 29-meric
Chain b 1–128（128 aa）
Not recorded PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 9 CDL CARDIOLIPIN × 5 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 4 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
5XTD Cryo-EM structure of human respiratory complex I Deposited 2017-06-19 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain b 1–128（128 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 9 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 CDL CARDIOLIPIN × 5 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
5XTH Cryo-EM structure of human respiratory supercomplex I1III2IV1 Deposited 2017-06-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain b 3–126（124 aa） Fragment：UNP RESIDUES 3-126
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 12 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 4 CDL CARDIOLIPIN × 14 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 10 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 HEA HEME-A × 2 ZN ZINC ION × 1 HEC HEME C × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
5XTI Cryo-EM architecture of human respiratory chain megacomplex-I2III2IV2 Deposited 2017-06-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 138 PDB declaration: 138-meric
Chain Bb 3–126（124 aa） Fragment：UNP RESIDUES 3-126
Chain b 3–126（124 aa） Fragment：UNP RESIDUES 3-126
Not recorded SF4 IRON/SULFUR CLUSTER × 12 FMN FLAVIN MONONUCLEOTIDE × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 21 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 4 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 6 CDL CARDIOLIPIN × 19 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 14 CU COPPER (II) ION × 6 MG MAGNESIUM ION × 2 HEA HEME-A × 4 ZN ZINC ION × 2 HEC HEME C × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 17.40 Å
9I4I High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-01-24 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain b 1–128（128 aa）
Not recorded PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 CDL CARDIOLIPIN × 6 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.63 Å
9TI4 High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-12-04 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain b 2–122（121 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 ZN ZINC ION × 1 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；25 mM HEPES-KOH pH=7.5, 50 mM KCl, 20 mM Mg(OAc)2, 0.01% (v/v) Lauryl Maltose Neopentyl Glycol (LMNG), 0.001 % cardiolipin, 0.001 % glycol-diosgenin (GDN, Sigma-Aldrich), 0.1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.66 Å

6 other PDB entries and 6 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUB6_HUMAN
Isoform —
PDB entities 25
Chains and sequence ranges Author chain b； PDBConstruct 1–128； UniProt 1–128

NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial

OrganismNot specified

UniProt O95169

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain c； UniProt 1–186 Not recorded NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P49821） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （O00217） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （O75251） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （P56556） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （O43678） Acyl carrier protein, mitochondrial × 2 （O14561） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q16718） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （O95182） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q16795） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （O43181） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P28331） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q9UI09） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P19404） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （O75489） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （O75306） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O15239） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （O75380） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （O95167） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q86Y39） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9P0J0） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （O95178） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （O43676） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （O43674） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （O95139） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （O96000） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q9NX14） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （O43677） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （O95298） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （O43920） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03897） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03901） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03915） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03923） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （O75438） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （O95168） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9Y6M9） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03905） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03886） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P51970） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （P17568） Unknow peptide × 1 NADH-ubiquinone oxidoreductase chain 2 × 1 （P03891） SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.44 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9CWT Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5XTC Cryo-EM structure of human respiratory complex I transmembrane arm Deposited 2017-06-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 29 PDB declaration: 29-meric
Chain c 34–186（153 aa）
Not recorded PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 9 CDL CARDIOLIPIN × 5 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 4 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
5XTD Cryo-EM structure of human respiratory complex I Deposited 2017-06-19 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain c 34–186（153 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 9 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 CDL CARDIOLIPIN × 5 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
5XTH Cryo-EM structure of human respiratory supercomplex I1III2IV1 Deposited 2017-06-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain c 34–186（153 aa） Fragment：UNP RESIDUES 34-186
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 12 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 4 CDL CARDIOLIPIN × 14 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 10 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 HEA HEME-A × 2 ZN ZINC ION × 1 HEC HEME C × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
5XTI Cryo-EM architecture of human respiratory chain megacomplex-I2III2IV2 Deposited 2017-06-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 138 PDB declaration: 138-meric
Chain Bc 34–186（153 aa） Fragment：UNP RESIDUES 34-186
Chain c 34–186（153 aa） Fragment：UNP RESIDUES 34-186
Not recorded SF4 IRON/SULFUR CLUSTER × 12 FMN FLAVIN MONONUCLEOTIDE × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 21 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 4 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 6 CDL CARDIOLIPIN × 19 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 14 CU COPPER (II) ION × 6 MG MAGNESIUM ION × 2 HEA HEME-A × 4 ZN ZINC ION × 2 HEC HEME C × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 17.40 Å
9I4I High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-01-24 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain c 1–186（186 aa）
Not recorded PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 CDL CARDIOLIPIN × 6 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.63 Å
9TI4 High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-12-04 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain c 31–182（152 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 ZN ZINC ION × 1 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；25 mM HEPES-KOH pH=7.5, 50 mM KCl, 20 mM Mg(OAc)2, 0.01% (v/v) Lauryl Maltose Neopentyl Glycol (LMNG), 0.001 % cardiolipin, 0.001 % glycol-diosgenin (GDN, Sigma-Aldrich), 0.1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.66 Å

6 other PDB entries and 6 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUB8_HUMAN
Isoform —
PDB entities 26
Chains and sequence ranges Author chain c； PDBConstruct 1–186； UniProt 1–186

NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10

OrganismNot specified

UniProt O96000

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain d； UniProt 1–172 Not recorded NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P49821） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （O00217） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （O75251） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （P56556） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （O43678） Acyl carrier protein, mitochondrial × 2 （O14561） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q16718） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （O95182） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q16795） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （O43181） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P28331） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q9UI09） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P19404） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （O75489） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （O75306） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O15239） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （O75380） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （O95167） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q86Y39） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9P0J0） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （O95178） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （O43676） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （O43674） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （O95139） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （O95169） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q9NX14） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （O43677） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （O95298） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （O43920） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03897） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03901） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03915） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03923） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （O75438） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （O95168） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9Y6M9） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03905） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03886） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P51970） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （P17568） Unknow peptide × 1 NADH-ubiquinone oxidoreductase chain 2 × 1 （P03891） SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.44 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9CWT Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5XTC Cryo-EM structure of human respiratory complex I transmembrane arm Deposited 2017-06-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 29 PDB declaration: 29-meric
Chain d 1–171（171 aa）
Not recorded PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 9 CDL CARDIOLIPIN × 5 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 4 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
5XTD Cryo-EM structure of human respiratory complex I Deposited 2017-06-19 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain d 1–171（171 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 9 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 CDL CARDIOLIPIN × 5 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
5XTH Cryo-EM structure of human respiratory supercomplex I1III2IV1 Deposited 2017-06-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain d 1–171（171 aa） Fragment：UNP RESIDUES 1-171
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 12 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 4 CDL CARDIOLIPIN × 14 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 10 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 HEA HEME-A × 2 ZN ZINC ION × 1 HEC HEME C × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
5XTI Cryo-EM architecture of human respiratory chain megacomplex-I2III2IV2 Deposited 2017-06-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 138 PDB declaration: 138-meric
Chain Bd 1–171（171 aa） Fragment：UNP RESIDUES 1-171
Chain d 1–171（171 aa） Fragment：UNP RESIDUES 1-171
Not recorded SF4 IRON/SULFUR CLUSTER × 12 FMN FLAVIN MONONUCLEOTIDE × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 21 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 4 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 6 CDL CARDIOLIPIN × 19 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 14 CU COPPER (II) ION × 6 MG MAGNESIUM ION × 2 HEA HEME-A × 4 ZN ZINC ION × 2 HEC HEME C × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 17.40 Å
9I4I High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-01-24 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain d 1–172（172 aa）
Not recorded PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 CDL CARDIOLIPIN × 6 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.63 Å
9TI4 High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-12-04 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain d 2–170（169 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 ZN ZINC ION × 1 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；25 mM HEPES-KOH pH=7.5, 50 mM KCl, 20 mM Mg(OAc)2, 0.01% (v/v) Lauryl Maltose Neopentyl Glycol (LMNG), 0.001 % cardiolipin, 0.001 % glycol-diosgenin (GDN, Sigma-Aldrich), 0.1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.66 Å

6 other PDB entries and 6 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUBA_HUMAN
Isoform —
PDB entities 27
Chains and sequence ranges Author chain d； PDBConstruct 1–172； UniProt 1–172

NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial

OrganismNot specified

UniProt Q9NX14

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain e； UniProt 1–153 Not recorded NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P49821） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （O00217） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （O75251） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （P56556） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （O43678） Acyl carrier protein, mitochondrial × 2 （O14561） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q16718） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （O95182） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q16795） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （O43181） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P28331） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q9UI09） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P19404） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （O75489） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （O75306） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O15239） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （O75380） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （O95167） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q86Y39） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9P0J0） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （O95178） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （O43676） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （O43674） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （O95139） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （O95169） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （O96000） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （O43677） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （O95298） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （O43920） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03897） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03901） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03915） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03923） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （O75438） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （O95168） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9Y6M9） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03905） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03886） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P51970） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （P17568） Unknow peptide × 1 NADH-ubiquinone oxidoreductase chain 2 × 1 （P03891） SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.44 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9CWT Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5XTC Cryo-EM structure of human respiratory complex I transmembrane arm Deposited 2017-06-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 29 PDB declaration: 29-meric
Chain e 54–150（97 aa） Fragment：UNP RESIDUES 54-150
Not recorded PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 9 CDL CARDIOLIPIN × 5 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 4 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
5XTD Cryo-EM structure of human respiratory complex I Deposited 2017-06-19 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain e 54–150（97 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 9 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 CDL CARDIOLIPIN × 5 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
5XTH Cryo-EM structure of human respiratory supercomplex I1III2IV1 Deposited 2017-06-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain e 54–150（97 aa） Fragment：UNP RESIDUES 54-150
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 12 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 4 CDL CARDIOLIPIN × 14 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 10 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 HEA HEME-A × 2 ZN ZINC ION × 1 HEC HEME C × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
5XTI Cryo-EM architecture of human respiratory chain megacomplex-I2III2IV2 Deposited 2017-06-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 138 PDB declaration: 138-meric
Chain Be 54–150（97 aa） Fragment：UNP RESIDUES 54-150
Chain e 54–150（97 aa） Fragment：UNP RESIDUES 54-150
Not recorded SF4 IRON/SULFUR CLUSTER × 12 FMN FLAVIN MONONUCLEOTIDE × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 21 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 4 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 6 CDL CARDIOLIPIN × 19 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 14 CU COPPER (II) ION × 6 MG MAGNESIUM ION × 2 HEA HEME-A × 4 ZN ZINC ION × 2 HEC HEME C × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 17.40 Å
9I4I High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-01-24 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain e 1–153（153 aa）
Not recorded PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 CDL CARDIOLIPIN × 6 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.63 Å
9TI4 High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-12-04 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain e 45–151（107 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 ZN ZINC ION × 1 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；25 mM HEPES-KOH pH=7.5, 50 mM KCl, 20 mM Mg(OAc)2, 0.01% (v/v) Lauryl Maltose Neopentyl Glycol (LMNG), 0.001 % cardiolipin, 0.001 % glycol-diosgenin (GDN, Sigma-Aldrich), 0.1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.66 Å

6 other PDB entries and 6 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUBB_HUMAN
Isoform —
PDB entities 28
Chains and sequence ranges Author chain e； PDBConstruct 1–153； UniProt 1–153

NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial

OrganismNot specified

UniProt O43677

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain f； UniProt 1–76 Not recorded NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P49821） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （O00217） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （O75251） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （P56556） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （O43678） Acyl carrier protein, mitochondrial × 2 （O14561） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q16718） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （O95182） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q16795） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （O43181） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P28331） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q9UI09） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P19404） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （O75489） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （O75306） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O15239） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （O75380） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （O95167） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q86Y39） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9P0J0） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （O95178） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （O43676） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （O43674） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （O95139） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （O95169） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （O96000） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q9NX14） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （O95298） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （O43920） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03897） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03901） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03915） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03923） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （O75438） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （O95168） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9Y6M9） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03905） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03886） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P51970） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （P17568） Unknow peptide × 1 NADH-ubiquinone oxidoreductase chain 2 × 1 （P03891） SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.44 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9CWT Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5XTC Cryo-EM structure of human respiratory complex I transmembrane arm Deposited 2017-06-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 29 PDB declaration: 29-meric
Chain f 28–74（47 aa）
Not recorded PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 9 CDL CARDIOLIPIN × 5 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 4 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
5XTD Cryo-EM structure of human respiratory complex I Deposited 2017-06-19 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain f 28–74（47 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 9 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 CDL CARDIOLIPIN × 5 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
5XTH Cryo-EM structure of human respiratory supercomplex I1III2IV1 Deposited 2017-06-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain f 28–74（47 aa） Fragment：UNP RESIDUES 28-74
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 12 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 4 CDL CARDIOLIPIN × 14 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 10 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 HEA HEME-A × 2 ZN ZINC ION × 1 HEC HEME C × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
5XTI Cryo-EM architecture of human respiratory chain megacomplex-I2III2IV2 Deposited 2017-06-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 138 PDB declaration: 138-meric
Chain Bf 28–74（47 aa） Fragment：UNP RESIDUES 28-74
Chain f 28–74（47 aa） Fragment：UNP RESIDUES 28-74
Not recorded SF4 IRON/SULFUR CLUSTER × 12 FMN FLAVIN MONONUCLEOTIDE × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 21 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 4 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 6 CDL CARDIOLIPIN × 19 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 14 CU COPPER (II) ION × 6 MG MAGNESIUM ION × 2 HEA HEME-A × 4 ZN ZINC ION × 2 HEC HEME C × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 17.40 Å
9I4I High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-01-24 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain f 1–76（76 aa）
Not recorded PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 CDL CARDIOLIPIN × 6 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.63 Å
9TI4 High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-12-04 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain f 27–75（49 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 ZN ZINC ION × 1 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；25 mM HEPES-KOH pH=7.5, 50 mM KCl, 20 mM Mg(OAc)2, 0.01% (v/v) Lauryl Maltose Neopentyl Glycol (LMNG), 0.001 % cardiolipin, 0.001 % glycol-diosgenin (GDN, Sigma-Aldrich), 0.1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.66 Å

6 other PDB entries and 6 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUC1_HUMAN
Isoform —
PDB entities 29
Chains and sequence ranges Author chain f； PDBConstruct 1–76； UniProt 1–76

NADH dehydrogenase [ubiquinone] 1 subunit C2

OrganismNot specified

UniProt O95298

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain g； UniProt 1–119 Not recorded NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P49821） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （O00217） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （O75251） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （P56556） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （O43678） Acyl carrier protein, mitochondrial × 2 （O14561） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q16718） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （O95182） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q16795） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （O43181） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P28331） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q9UI09） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P19404） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （O75489） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （O75306） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O15239） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （O75380） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （O95167） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q86Y39） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9P0J0） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （O95178） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （O43676） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （O43674） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （O95139） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （O95169） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （O96000） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q9NX14） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （O43677） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （O43920） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03897） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03901） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03915） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03923） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （O75438） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （O95168） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9Y6M9） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03905） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03886） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P51970） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （P17568） Unknow peptide × 1 NADH-ubiquinone oxidoreductase chain 2 × 1 （P03891） SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.44 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9CWT Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5XTC Cryo-EM structure of human respiratory complex I transmembrane arm Deposited 2017-06-19 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 29 PDB declaration: 29-meric
Chain g 1–119（119 aa）
Not recorded PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 9 CDL CARDIOLIPIN × 5 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 4 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
5XTD Cryo-EM structure of human respiratory complex I Deposited 2017-06-19 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain g 1–119（119 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 9 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 CDL CARDIOLIPIN × 5 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
5XTH Cryo-EM structure of human respiratory supercomplex I1III2IV1 Deposited 2017-06-19 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain g 1–119（119 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 12 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 4 CDL CARDIOLIPIN × 14 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 10 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 HEA HEME-A × 2 ZN ZINC ION × 1 HEC HEME C × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
5XTI Cryo-EM architecture of human respiratory chain megacomplex-I2III2IV2 Deposited 2017-06-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 138 PDB declaration: 138-meric
Chain Bg 1–119（119 aa）
Chain g 1–119（119 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 12 FMN FLAVIN MONONUCLEOTIDE × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 21 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 4 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 6 CDL CARDIOLIPIN × 19 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 14 CU COPPER (II) ION × 6 MG MAGNESIUM ION × 2 HEA HEME-A × 4 ZN ZINC ION × 2 HEC HEME C × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 17.40 Å
9I4I High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-01-24 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain g 1–119（119 aa）
Not recorded PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 CDL CARDIOLIPIN × 6 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.63 Å
9TI4 High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-12-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain g 1–119（119 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 ZN ZINC ION × 1 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；25 mM HEPES-KOH pH=7.5, 50 mM KCl, 20 mM Mg(OAc)2, 0.01% (v/v) Lauryl Maltose Neopentyl Glycol (LMNG), 0.001 % cardiolipin, 0.001 % glycol-diosgenin (GDN, Sigma-Aldrich), 0.1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.66 Å

6 other PDB entries and 6 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUC2_HUMAN
Isoform —
PDB entities 30
Chains and sequence ranges Author chain g； PDBConstruct 1–119； UniProt 1–119

NADH dehydrogenase [ubiquinone] iron-sulfur protein 5

OrganismNot specified

UniProt O43920

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain h； UniProt 1–106 Not recorded NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P49821） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （O00217） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （O75251） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （P56556） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （O43678） Acyl carrier protein, mitochondrial × 2 （O14561） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q16718） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （O95182） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q16795） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （O43181） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P28331） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q9UI09） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P19404） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （O75489） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （O75306） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O15239） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （O75380） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （O95167） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q86Y39） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9P0J0） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （O95178） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （O43676） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （O43674） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （O95139） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （O95169） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （O96000） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q9NX14） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （O43677） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （O95298） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03897） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03901） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03915） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03923） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （O75438） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （O95168） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9Y6M9） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03905） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03886） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P51970） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （P17568） Unknow peptide × 1 NADH-ubiquinone oxidoreductase chain 2 × 1 （P03891） SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.44 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9CWT Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5XTC Cryo-EM structure of human respiratory complex I transmembrane arm Deposited 2017-06-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 29 PDB declaration: 29-meric
Chain h 2–105（104 aa）
Not recorded PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 9 CDL CARDIOLIPIN × 5 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 4 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
5XTD Cryo-EM structure of human respiratory complex I Deposited 2017-06-19 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain h 2–105（104 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 9 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 CDL CARDIOLIPIN × 5 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
5XTH Cryo-EM structure of human respiratory supercomplex I1III2IV1 Deposited 2017-06-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain h 2–105（104 aa） Fragment：UNP RESIDUES 2-105
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 12 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 4 CDL CARDIOLIPIN × 14 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 10 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 HEA HEME-A × 2 ZN ZINC ION × 1 HEC HEME C × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
5XTI Cryo-EM architecture of human respiratory chain megacomplex-I2III2IV2 Deposited 2017-06-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 138 PDB declaration: 138-meric
Chain Bh 2–105（104 aa） Fragment：UNP RESIDUES 2-105
Chain h 2–105（104 aa） Fragment：UNP RESIDUES 2-105
Not recorded SF4 IRON/SULFUR CLUSTER × 12 FMN FLAVIN MONONUCLEOTIDE × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 21 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 4 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 6 CDL CARDIOLIPIN × 19 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 14 CU COPPER (II) ION × 6 MG MAGNESIUM ION × 2 HEA HEME-A × 4 ZN ZINC ION × 2 HEC HEME C × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 17.40 Å
9I4I High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-01-24 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain h 1–106（106 aa）
Not recorded PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 CDL CARDIOLIPIN × 6 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.63 Å
9TI4 High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-12-04 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain h 1–105（105 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 ZN ZINC ION × 1 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；25 mM HEPES-KOH pH=7.5, 50 mM KCl, 20 mM Mg(OAc)2, 0.01% (v/v) Lauryl Maltose Neopentyl Glycol (LMNG), 0.001 % cardiolipin, 0.001 % glycol-diosgenin (GDN, Sigma-Aldrich), 0.1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.66 Å

6 other PDB entries and 6 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUS5_HUMAN
Isoform —
PDB entities 31
Chains and sequence ranges Author chain h； PDBConstruct 1–106； UniProt 1–106

NADH-ubiquinone oxidoreductase chain 3

OrganismNot specified

UniProt P03897

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain j； UniProt 1–115 Not recorded NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P49821） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （O00217） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （O75251） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （P56556） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （O43678） Acyl carrier protein, mitochondrial × 2 （O14561） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q16718） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （O95182） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q16795） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （O43181） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P28331） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q9UI09） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P19404） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （O75489） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （O75306） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O15239） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （O75380） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （O95167） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q86Y39） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9P0J0） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （O95178） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （O43676） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （O43674） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （O95139） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （O95169） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （O96000） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q9NX14） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （O43677） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （O95298） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （O43920） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03901） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03915） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03923） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （O75438） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （O95168） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9Y6M9） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03905） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03886） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P51970） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （P17568） Unknow peptide × 1 NADH-ubiquinone oxidoreductase chain 2 × 1 （P03891） SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.44 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9CWT Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
9I4I High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-01-24 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain j 1–115（115 aa）
Not recorded PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 CDL CARDIOLIPIN × 6 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.63 Å
9TI4 High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-12-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain j 1–115（115 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 ZN ZINC ION × 1 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；25 mM HEPES-KOH pH=7.5, 50 mM KCl, 20 mM Mg(OAc)2, 0.01% (v/v) Lauryl Maltose Neopentyl Glycol (LMNG), 0.001 % cardiolipin, 0.001 % glycol-diosgenin (GDN, Sigma-Aldrich), 0.1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.66 Å

2 other PDB entries and 2 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NU3M_HUMAN
Isoform —
PDB entities 32
Chains and sequence ranges Author chain j； PDBConstruct 1–115； UniProt 1–115

NADH-ubiquinone oxidoreductase chain 4L

OrganismNot specified

UniProt P03901

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain k； UniProt 1–98 Not recorded NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P49821） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （O00217） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （O75251） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （P56556） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （O43678） Acyl carrier protein, mitochondrial × 2 （O14561） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q16718） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （O95182） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q16795） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （O43181） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P28331） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q9UI09） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P19404） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （O75489） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （O75306） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O15239） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （O75380） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （O95167） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q86Y39） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9P0J0） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （O95178） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （O43676） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （O43674） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （O95139） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （O95169） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （O96000） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q9NX14） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （O43677） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （O95298） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （O43920） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03897） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03915） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03923） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （O75438） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （O95168） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9Y6M9） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03905） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03886） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P51970） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （P17568） Unknow peptide × 1 NADH-ubiquinone oxidoreductase chain 2 × 1 （P03891） SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.44 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9CWT Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
9I4I High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-01-24 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain k 1–98（98 aa）
Not recorded PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 CDL CARDIOLIPIN × 6 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.63 Å
9TI4 High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-12-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain k 1–98（98 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 ZN ZINC ION × 1 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；25 mM HEPES-KOH pH=7.5, 50 mM KCl, 20 mM Mg(OAc)2, 0.01% (v/v) Lauryl Maltose Neopentyl Glycol (LMNG), 0.001 % cardiolipin, 0.001 % glycol-diosgenin (GDN, Sigma-Aldrich), 0.1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.66 Å

2 other PDB entries and 2 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NU4LM_HUMAN
Isoform —
PDB entities 33
Chains and sequence ranges Author chain k； PDBConstruct 1–98； UniProt 1–98

NADH-ubiquinone oxidoreductase chain 5

OrganismNot specified

UniProt P03915

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain l； UniProt 1–603 Not recorded NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P49821） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （O00217） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （O75251） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （P56556） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （O43678） Acyl carrier protein, mitochondrial × 2 （O14561） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q16718） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （O95182） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q16795） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （O43181） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P28331） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q9UI09） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P19404） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （O75489） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （O75306） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O15239） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （O75380） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （O95167） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q86Y39） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9P0J0） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （O95178） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （O43676） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （O43674） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （O95139） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （O95169） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （O96000） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q9NX14） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （O43677） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （O95298） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （O43920） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03897） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03901） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03923） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （O75438） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （O95168） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9Y6M9） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03905） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03886） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P51970） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （P17568） Unknow peptide × 1 NADH-ubiquinone oxidoreductase chain 2 × 1 （P03891） SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.44 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9CWT Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
9I4I High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-01-24 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain l 1–603（603 aa）
Not recorded PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 CDL CARDIOLIPIN × 6 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.63 Å
9TI4 High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-12-04 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain l 3–603（601 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 ZN ZINC ION × 1 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；25 mM HEPES-KOH pH=7.5, 50 mM KCl, 20 mM Mg(OAc)2, 0.01% (v/v) Lauryl Maltose Neopentyl Glycol (LMNG), 0.001 % cardiolipin, 0.001 % glycol-diosgenin (GDN, Sigma-Aldrich), 0.1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.66 Å

2 other PDB entries and 2 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NU5M_HUMAN
Isoform —
PDB entities 34
Chains and sequence ranges Author chain l； PDBConstruct 1–603； UniProt 1–603

NADH-ubiquinone oxidoreductase chain 6

OrganismNot specified

UniProt P03923

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain m； UniProt 1–174 Not recorded NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P49821） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （O00217） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （O75251） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （P56556） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （O43678） Acyl carrier protein, mitochondrial × 2 （O14561） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q16718） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （O95182） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q16795） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （O43181） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P28331） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q9UI09） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P19404） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （O75489） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （O75306） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O15239） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （O75380） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （O95167） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q86Y39） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9P0J0） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （O95178） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （O43676） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （O43674） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （O95139） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （O95169） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （O96000） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q9NX14） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （O43677） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （O95298） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （O43920） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03897） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03901） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03915） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （O75438） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （O95168） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9Y6M9） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03905） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03886） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P51970） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （P17568） Unknow peptide × 1 NADH-ubiquinone oxidoreductase chain 2 × 1 （P03891） SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.44 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9CWT Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
9I4I High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-01-24 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain m 1–174（174 aa）
Not recorded PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 CDL CARDIOLIPIN × 6 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.63 Å
9TI4 High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-12-04 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain m 1–173（173 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 ZN ZINC ION × 1 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；25 mM HEPES-KOH pH=7.5, 50 mM KCl, 20 mM Mg(OAc)2, 0.01% (v/v) Lauryl Maltose Neopentyl Glycol (LMNG), 0.001 % cardiolipin, 0.001 % glycol-diosgenin (GDN, Sigma-Aldrich), 0.1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.66 Å

2 other PDB entries and 2 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NU6M_HUMAN
Isoform —
PDB entities 35
Chains and sequence ranges Author chain m； PDBConstruct 1–174； UniProt 1–174

NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1

OrganismNot specified

UniProt O75438

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain n； UniProt 1–58 Not recorded NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P49821） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （O00217） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （O75251） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （P56556） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （O43678） Acyl carrier protein, mitochondrial × 2 （O14561） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q16718） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （O95182） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q16795） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （O43181） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P28331） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q9UI09） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P19404） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （O75489） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （O75306） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O15239） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （O75380） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （O95167） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q86Y39） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9P0J0） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （O95178） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （O43676） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （O43674） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （O95139） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （O95169） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （O96000） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q9NX14） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （O43677） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （O95298） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （O43920） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03897） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03901） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03915） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03923） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （O95168） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9Y6M9） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03905） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03886） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P51970） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （P17568） Unknow peptide × 1 NADH-ubiquinone oxidoreductase chain 2 × 1 （P03891） SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.44 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9CWT Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5XTC Cryo-EM structure of human respiratory complex I transmembrane arm Deposited 2017-06-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 29 PDB declaration: 29-meric
Chain n 3–58（56 aa）
Not recorded PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 9 CDL CARDIOLIPIN × 5 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 4 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
5XTD Cryo-EM structure of human respiratory complex I Deposited 2017-06-19 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain n 3–58（56 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 9 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 CDL CARDIOLIPIN × 5 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
5XTH Cryo-EM structure of human respiratory supercomplex I1III2IV1 Deposited 2017-06-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain n 3–58（56 aa） Fragment：UNP RESIDUES 3-58
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 12 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 4 CDL CARDIOLIPIN × 14 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 10 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 HEA HEME-A × 2 ZN ZINC ION × 1 HEC HEME C × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
5XTI Cryo-EM architecture of human respiratory chain megacomplex-I2III2IV2 Deposited 2017-06-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 138 PDB declaration: 138-meric
Chain Bn 3–58（56 aa） Fragment：UNP RESIDUES 3-58
Chain n 3–58（56 aa） Fragment：UNP RESIDUES 3-58
Not recorded SF4 IRON/SULFUR CLUSTER × 12 FMN FLAVIN MONONUCLEOTIDE × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 21 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 4 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 6 CDL CARDIOLIPIN × 19 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 14 CU COPPER (II) ION × 6 MG MAGNESIUM ION × 2 HEA HEME-A × 4 ZN ZINC ION × 2 HEC HEME C × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 17.40 Å
9I4I High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-01-24 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain n 1–58（58 aa）
Not recorded PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 CDL CARDIOLIPIN × 6 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.63 Å
9TI4 High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-12-04 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain n 5–58（54 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 ZN ZINC ION × 1 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；25 mM HEPES-KOH pH=7.5, 50 mM KCl, 20 mM Mg(OAc)2, 0.01% (v/v) Lauryl Maltose Neopentyl Glycol (LMNG), 0.001 % cardiolipin, 0.001 % glycol-diosgenin (GDN, Sigma-Aldrich), 0.1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.66 Å

6 other PDB entries and 6 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUB1_HUMAN
Isoform —
PDB entities 36
Chains and sequence ranges Author chain n； PDBConstruct 1–58； UniProt 1–58

NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4

OrganismNot specified

UniProt O95168

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain o； UniProt 1–129 Not recorded NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P49821） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （O00217） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （O75251） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （P56556） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （O43678） Acyl carrier protein, mitochondrial × 2 （O14561） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q16718） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （O95182） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q16795） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （O43181） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P28331） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q9UI09） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P19404） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （O75489） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （O75306） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O15239） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （O75380） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （O95167） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q86Y39） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9P0J0） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （O95178） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （O43676） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （O43674） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （O95139） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （O95169） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （O96000） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q9NX14） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （O43677） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （O95298） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （O43920） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03897） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03901） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03915） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03923） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （O75438） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9Y6M9） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03905） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03886） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P51970） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （P17568） Unknow peptide × 1 NADH-ubiquinone oxidoreductase chain 2 × 1 （P03891） SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.44 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9CWT Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5XTC Cryo-EM structure of human respiratory complex I transmembrane arm Deposited 2017-06-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 29 PDB declaration: 29-meric
Chain o 2–129（128 aa）
Not recorded PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 9 CDL CARDIOLIPIN × 5 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 4 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
5XTD Cryo-EM structure of human respiratory complex I Deposited 2017-06-19 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain o 2–129（128 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 9 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 CDL CARDIOLIPIN × 5 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
5XTH Cryo-EM structure of human respiratory supercomplex I1III2IV1 Deposited 2017-06-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain o 2–129（128 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 12 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 4 CDL CARDIOLIPIN × 14 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 10 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 HEA HEME-A × 2 ZN ZINC ION × 1 HEC HEME C × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
5XTI Cryo-EM architecture of human respiratory chain megacomplex-I2III2IV2 Deposited 2017-06-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 138 PDB declaration: 138-meric
Chain Bo 2–129（128 aa）
Chain o 2–129（128 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 12 FMN FLAVIN MONONUCLEOTIDE × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 21 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 4 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 6 CDL CARDIOLIPIN × 19 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 14 CU COPPER (II) ION × 6 MG MAGNESIUM ION × 2 HEA HEME-A × 4 ZN ZINC ION × 2 HEC HEME C × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 17.40 Å
9I4I High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-01-24 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain o 1–129（129 aa）
Not recorded PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 CDL CARDIOLIPIN × 6 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.63 Å
9TI4 High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-12-04 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain o 2–129（128 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 ZN ZINC ION × 1 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；25 mM HEPES-KOH pH=7.5, 50 mM KCl, 20 mM Mg(OAc)2, 0.01% (v/v) Lauryl Maltose Neopentyl Glycol (LMNG), 0.001 % cardiolipin, 0.001 % glycol-diosgenin (GDN, Sigma-Aldrich), 0.1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.66 Å

6 other PDB entries and 6 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUB4_HUMAN
Isoform —
PDB entities 37
Chains and sequence ranges Author chain o； PDBConstruct 1–129； UniProt 1–129

NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9

OrganismNot specified

UniProt Q9Y6M9

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain p； UniProt 1–179 Not recorded NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P49821） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （O00217） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （O75251） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （P56556） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （O43678） Acyl carrier protein, mitochondrial × 2 （O14561） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q16718） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （O95182） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q16795） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （O43181） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P28331） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q9UI09） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P19404） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （O75489） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （O75306） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O15239） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （O75380） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （O95167） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q86Y39） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9P0J0） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （O95178） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （O43676） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （O43674） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （O95139） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （O95169） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （O96000） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q9NX14） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （O43677） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （O95298） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （O43920） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03897） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03901） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03915） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03923） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （O75438） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （O95168） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03905） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03886） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P51970） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （P17568） Unknow peptide × 1 NADH-ubiquinone oxidoreductase chain 2 × 1 （P03891） SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.44 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9CWT Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5XTC Cryo-EM structure of human respiratory complex I transmembrane arm Deposited 2017-06-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 29 PDB declaration: 29-meric
Chain p 8–179（172 aa）
Not recorded PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 9 CDL CARDIOLIPIN × 5 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 4 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
5XTD Cryo-EM structure of human respiratory complex I Deposited 2017-06-19 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain p 8–179（172 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 9 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 CDL CARDIOLIPIN × 5 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
5XTH Cryo-EM structure of human respiratory supercomplex I1III2IV1 Deposited 2017-06-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain p 8–179（172 aa） Fragment：UNP RESIDUES 8-179
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 12 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 4 CDL CARDIOLIPIN × 14 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 10 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 HEA HEME-A × 2 ZN ZINC ION × 1 HEC HEME C × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
5XTI Cryo-EM architecture of human respiratory chain megacomplex-I2III2IV2 Deposited 2017-06-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 138 PDB declaration: 138-meric
Chain Bp 8–179（172 aa） Fragment：UNP RESIDUES 8-179
Chain p 8–179（172 aa） Fragment：UNP RESIDUES 8-179
Not recorded SF4 IRON/SULFUR CLUSTER × 12 FMN FLAVIN MONONUCLEOTIDE × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 21 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 4 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 6 CDL CARDIOLIPIN × 19 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 14 CU COPPER (II) ION × 6 MG MAGNESIUM ION × 2 HEA HEME-A × 4 ZN ZINC ION × 2 HEC HEME C × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 17.40 Å
9I4I High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-01-24 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain p 1–179（179 aa）
Not recorded PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 CDL CARDIOLIPIN × 6 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.63 Å
9TI4 High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-12-04 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain p 7–179（173 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 ZN ZINC ION × 1 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；25 mM HEPES-KOH pH=7.5, 50 mM KCl, 20 mM Mg(OAc)2, 0.01% (v/v) Lauryl Maltose Neopentyl Glycol (LMNG), 0.001 % cardiolipin, 0.001 % glycol-diosgenin (GDN, Sigma-Aldrich), 0.1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.66 Å

6 other PDB entries and 6 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUB9_HUMAN
Isoform —
PDB entities 38
Chains and sequence ranges Author chain p； PDBConstruct 1–179； UniProt 1–179

NADH-ubiquinone oxidoreductase chain 4

OrganismNot specified

UniProt P03905

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain r； UniProt 1–459 Not recorded NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P49821） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （O00217） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （O75251） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （P56556） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （O43678） Acyl carrier protein, mitochondrial × 2 （O14561） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q16718） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （O95182） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q16795） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （O43181） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P28331） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q9UI09） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P19404） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （O75489） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （O75306） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O15239） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （O75380） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （O95167） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q86Y39） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9P0J0） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （O95178） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （O43676） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （O43674） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （O95139） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （O95169） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （O96000） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q9NX14） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （O43677） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （O95298） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （O43920） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03897） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03901） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03915） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03923） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （O75438） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （O95168） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9Y6M9） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03886） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P51970） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （P17568） Unknow peptide × 1 NADH-ubiquinone oxidoreductase chain 2 × 1 （P03891） SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.44 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9CWT Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
9I4I High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-01-24 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain r 1–459（459 aa）
Not recorded PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 CDL CARDIOLIPIN × 6 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.63 Å
9TI4 High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-12-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain r 1–459（459 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 ZN ZINC ION × 1 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；25 mM HEPES-KOH pH=7.5, 50 mM KCl, 20 mM Mg(OAc)2, 0.01% (v/v) Lauryl Maltose Neopentyl Glycol (LMNG), 0.001 % cardiolipin, 0.001 % glycol-diosgenin (GDN, Sigma-Aldrich), 0.1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.66 Å

2 other PDB entries and 2 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NU4M_HUMAN
Isoform —
PDB entities 39
Chains and sequence ranges Author chain r； PDBConstruct 1–459； UniProt 1–459

NADH-ubiquinone oxidoreductase chain 1

OrganismNot specified

UniProt P03886

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain s； UniProt 1–318 Not recorded NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P49821） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （O00217） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （O75251） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （P56556） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （O43678） Acyl carrier protein, mitochondrial × 2 （O14561） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q16718） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （O95182） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q16795） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （O43181） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P28331） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q9UI09） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P19404） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （O75489） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （O75306） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O15239） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （O75380） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （O95167） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q86Y39） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9P0J0） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （O95178） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （O43676） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （O43674） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （O95139） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （O95169） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （O96000） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q9NX14） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （O43677） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （O95298） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （O43920） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03897） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03901） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03915） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03923） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （O75438） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （O95168） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9Y6M9） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03905） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P51970） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （P17568） Unknow peptide × 1 NADH-ubiquinone oxidoreductase chain 2 × 1 （P03891） SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.44 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9CWT Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
9I4I High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-01-24 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain s 1–318（318 aa）
Not recorded PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 CDL CARDIOLIPIN × 6 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.63 Å
9TI4 High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-12-04 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain s 3–318（316 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 ZN ZINC ION × 1 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；25 mM HEPES-KOH pH=7.5, 50 mM KCl, 20 mM Mg(OAc)2, 0.01% (v/v) Lauryl Maltose Neopentyl Glycol (LMNG), 0.001 % cardiolipin, 0.001 % glycol-diosgenin (GDN, Sigma-Aldrich), 0.1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.66 Å

2 other PDB entries and 2 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NU1M_HUMAN
Isoform —
PDB entities 40
Chains and sequence ranges Author chain s； PDBConstruct 1–318； UniProt 1–318

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8

OrganismNot specified

UniProt P51970

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain u； UniProt 1–172 Not recorded NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P49821） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （O00217） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （O75251） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （P56556） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （O43678） Acyl carrier protein, mitochondrial × 2 （O14561） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q16718） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （O95182） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q16795） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （O43181） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P28331） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q9UI09） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P19404） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （O75489） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （O75306） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O15239） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （O75380） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （O95167） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q86Y39） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9P0J0） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （O95178） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （O43676） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （O43674） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （O95139） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （O95169） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （O96000） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q9NX14） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （O43677） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （O95298） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （O43920） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03897） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03901） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03915） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03923） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （O75438） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （O95168） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9Y6M9） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03905） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03886） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （P17568） Unknow peptide × 1 NADH-ubiquinone oxidoreductase chain 2 × 1 （P03891） SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.44 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9CWT Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5XTC Cryo-EM structure of human respiratory complex I transmembrane arm Deposited 2017-06-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 29 PDB declaration: 29-meric
Chain u 4–172（169 aa）
Not recorded PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 9 CDL CARDIOLIPIN × 5 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 4 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
5XTD Cryo-EM structure of human respiratory complex I Deposited 2017-06-19 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain u 4–172（169 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 9 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 CDL CARDIOLIPIN × 5 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
5XTH Cryo-EM structure of human respiratory supercomplex I1III2IV1 Deposited 2017-06-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain u 4–172（169 aa） Fragment：UNP RESIDUES 4-172
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 12 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 4 CDL CARDIOLIPIN × 14 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 10 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 HEA HEME-A × 2 ZN ZINC ION × 1 HEC HEME C × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
5XTI Cryo-EM architecture of human respiratory chain megacomplex-I2III2IV2 Deposited 2017-06-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 138 PDB declaration: 138-meric
Chain Bu 4–172（169 aa） Fragment：UNP RESIDUES 4-172
Chain u 4–172（169 aa） Fragment：UNP RESIDUES 4-172
Not recorded SF4 IRON/SULFUR CLUSTER × 12 FMN FLAVIN MONONUCLEOTIDE × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 21 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 4 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 6 CDL CARDIOLIPIN × 19 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 14 CU COPPER (II) ION × 6 MG MAGNESIUM ION × 2 HEA HEME-A × 4 ZN ZINC ION × 2 HEC HEME C × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 17.40 Å
9I4I High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-01-24 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain u 1–172（172 aa）
Not recorded PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 CDL CARDIOLIPIN × 6 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.63 Å
9TI4 High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-12-04 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain u 2–172（171 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 ZN ZINC ION × 1 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；25 mM HEPES-KOH pH=7.5, 50 mM KCl, 20 mM Mg(OAc)2, 0.01% (v/v) Lauryl Maltose Neopentyl Glycol (LMNG), 0.001 % cardiolipin, 0.001 % glycol-diosgenin (GDN, Sigma-Aldrich), 0.1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.66 Å

6 other PDB entries and 6 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUA8_HUMAN
Isoform —
PDB entities 41
Chains and sequence ranges Author chain u； PDBConstruct 1–172； UniProt 1–172

NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7

OrganismNot specified

UniProt P17568

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain v； UniProt 1–137 Not recorded NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P49821） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （O00217） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （O75251） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （P56556） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （O43678） Acyl carrier protein, mitochondrial × 2 （O14561） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q16718） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （O95182） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q16795） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （O43181） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P28331） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q9UI09） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P19404） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （O75489） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （O75306） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O15239） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （O75380） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （O95167） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q86Y39） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9P0J0） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （O95178） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （O43676） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （O43674） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （O95139） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （O95169） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （O96000） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q9NX14） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （O43677） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （O95298） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （O43920） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03897） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03901） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03915） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03923） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （O75438） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （O95168） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9Y6M9） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03905） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03886） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P51970） Unknow peptide × 1 NADH-ubiquinone oxidoreductase chain 2 × 1 （P03891） SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.44 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9CWT Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5XTC Cryo-EM structure of human respiratory complex I transmembrane arm Deposited 2017-06-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 29 PDB declaration: 29-meric
Chain v 3–124（122 aa）
Not recorded PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 9 CDL CARDIOLIPIN × 5 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 4 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
5XTD Cryo-EM structure of human respiratory complex I Deposited 2017-06-19 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain v 1–137（137 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 9 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 CDL CARDIOLIPIN × 5 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
5XTH Cryo-EM structure of human respiratory supercomplex I1III2IV1 Deposited 2017-06-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain v 3–124（122 aa） Fragment：UNP RESIDUES 3-124
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 12 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 4 CDL CARDIOLIPIN × 14 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 10 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 HEA HEME-A × 2 ZN ZINC ION × 1 HEC HEME C × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
5XTI Cryo-EM architecture of human respiratory chain megacomplex-I2III2IV2 Deposited 2017-06-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 138 PDB declaration: 138-meric
Chain Bv 3–124（122 aa） Fragment：UNP RESIDUES 3-124
Chain v 3–124（122 aa） Fragment：UNP RESIDUES 3-124
Not recorded SF4 IRON/SULFUR CLUSTER × 12 FMN FLAVIN MONONUCLEOTIDE × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 21 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 4 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 6 CDL CARDIOLIPIN × 19 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 14 CU COPPER (II) ION × 6 MG MAGNESIUM ION × 2 HEA HEME-A × 4 ZN ZINC ION × 2 HEC HEME C × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 17.40 Å
9I4I High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-01-24 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain v 1–137（137 aa）
Not recorded PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 CDL CARDIOLIPIN × 6 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.63 Å
9TI4 High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-12-04 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain v 2–122（121 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 ZN ZINC ION × 1 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；25 mM HEPES-KOH pH=7.5, 50 mM KCl, 20 mM Mg(OAc)2, 0.01% (v/v) Lauryl Maltose Neopentyl Glycol (LMNG), 0.001 % cardiolipin, 0.001 % glycol-diosgenin (GDN, Sigma-Aldrich), 0.1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.66 Å

6 other PDB entries and 6 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUB7_HUMAN
Isoform —
PDB entities 42
Chains and sequence ranges Author chain v； PDBConstruct 1–137； UniProt 1–137

NADH-ubiquinone oxidoreductase chain 2

OrganismNot specified

UniProt P03891

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain i； UniProt 1–347 Not recorded NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P49821） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （O00217） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （O75251） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （P56556） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （O43678） Acyl carrier protein, mitochondrial × 2 （O14561） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （Q16718） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （O95182） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （Q16795） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （O43181） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P28331） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （Q9UI09） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P19404） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （O75489） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （O75306） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （O15239） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （O75380） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （O95167） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q86Y39） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q9P0J0） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （O95178） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （O43676） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （O43674） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （O95139） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （O95169） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （O96000） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q9NX14） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （O43677） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （O95298） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （O43920） NADH-ubiquinone oxidoreductase chain 3 × 1 （P03897） NADH-ubiquinone oxidoreductase chain 4L × 1 （P03901） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03915） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03923） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （O75438） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （O95168） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q9Y6M9） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03905） NADH-ubiquinone oxidoreductase chain 1 × 1 （P03886） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P51970） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （P17568） Unknow peptide × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.44 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9CWT Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
9I4I High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-01-24 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain i 1–347（347 aa）
Not recorded PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 CDL CARDIOLIPIN × 6 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.63 Å
9TI4 High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-12-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain i 1–347（347 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 ZN ZINC ION × 1 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；25 mM HEPES-KOH pH=7.5, 50 mM KCl, 20 mM Mg(OAc)2, 0.01% (v/v) Lauryl Maltose Neopentyl Glycol (LMNG), 0.001 % cardiolipin, 0.001 % glycol-diosgenin (GDN, Sigma-Aldrich), 0.1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.66 Å

2 other PDB entries and 2 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NU2M_HUMAN
Isoform —
PDB entities 44
Chains and sequence ranges Author chain i； PDBConstruct 1–347； UniProt 1–347

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 9cwt

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 9cwt
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▼

2. Structure Basics 2. Structure Basics

Entry ID entry_id9cwt
Deposition date deposition_date2024-07-30
Structure title titleHuman kidney respiratory complex I
Keywords keywordshuman, kidney, respiratory complex I, MEMBRANE PROTEIN; MEMBRANE PROTEIN
Experimental Method methodELECTRON MICROSCOPY
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier82.78 Å
Radius of gyration Rg (electron density) rg_electron82.86 Å
Forward intensity I(0) i09702080000.00
Molecular weight molecular_weight866940.0 kDa
Excluded volume excluded_volume1095800 ų
Envelope volume envelope_volume1613200 ų
Hydration-shell volume shell_volume165320 ų
Envelope diameter envelope_diameter291.5 Å
Shell Rg shell_rg78.35 Å
Envelope Rg envelope_rg81.03 Å
Shape Rg shape_rg82.88 Å
Total Rg total_rg82.76 Å
Total atoms total_atoms60914
Residues n_residues7624
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax277.1 Å
Rg (real space) rg_real86.65 Å
Rg uncertainty (real space) rg_real_error1.67 Å
I(0) (real space) i0_real9.7020e+09
I(0) uncertainty (real space) i0_real_error2.0060e+08
Rg (reciprocal space) rg_reciprocal80.37 Å
I(0) (reciprocal space) i0_reciprocal9635000000.0000
Solution quality estimate total_estimate0.8553
Solution quality rating solution_quality GOOD a GOOD solution
P(r) peaks n_peaks1
Primary peak position r_peak_primary58.3 Å
Skewness Skewness skewness0.463
Kurtosis Kurtosis kurtosis-0.538
Angular range angular_range— – 0.0950 Å−1
Current regularization parameter α current_alpha1.6800
Highest regularization parameter α highest_alpha476300000.0000
Real-space data points n_real_points20
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.830; Stabil: 0.897; Sysdev: 1.000; Positv: 1.000; Valcen: 0.926; Smooth: 0.046
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (49)

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8. Citations (1)

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9. Files and Curves (10)