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9w2v

Cryo-EM structure of complex I on the bovine heart submitochondrial particles, closed

Method: ELECTRON MICROSCOPY Dmax: 221.3 Å Quality: EXCELLENT
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1. Protein Identity and Related Structures Protein Identity & Related Structures

NADH-ubiquinone oxidoreductase chain 3

Bos taurus

UniProt Q8WAB4

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain A； UniProt 2–115 Not recorded NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （P42026） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （P23709） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （P17694） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P04394） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P25708） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P15690） NADH-ubiquinone oxidoreductase chain 1 × 1 （Q6EMT1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （P42028） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03924） NADH-ubiquinone oxidoreductase chain 4L × 1 （Q6EMS3） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03920） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03910） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03892） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （P34942） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （P34943） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q02375） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P23934） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q02370） Acyl carrier protein, mitochondrial × 1 （Q9CR21） Acyl carrier protein, mitochondrial × 1 （P52505） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （P23935） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q02366） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P42029） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q8HXG6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q95KV7） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （Q02377） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q02371） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q02376） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q02827） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q02379） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （Q02378） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q8HXG5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q02380） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q02367） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q02374） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q02365） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q02372） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （P48305） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q02369） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q02368） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q02373） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （O97725） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q05752） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） FME N-FORMYLMETHIONINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 2.80 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9W2V Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
9W2U Cryo-EM structure of complex I on the bovine heart submitochondrial particles, open Deposited 2025-07-28 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain A 2–115（114 aa）
Not recorded FME N-FORMYLMETHIONINE × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NU3M_BOSIN
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 1–114； UniProt 2–115

NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial

Bos taurus

UniProt P42026

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain B； UniProt 62–216 Not recorded NADH-ubiquinone oxidoreductase chain 3 × 1 （Q8WAB4） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （P23709） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （P17694） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P04394） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P25708） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P15690） NADH-ubiquinone oxidoreductase chain 1 × 1 （Q6EMT1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （P42028） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03924） NADH-ubiquinone oxidoreductase chain 4L × 1 （Q6EMS3） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03920） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03910） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03892） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （P34942） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （P34943） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q02375） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P23934） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q02370） Acyl carrier protein, mitochondrial × 1 （Q9CR21） Acyl carrier protein, mitochondrial × 1 （P52505） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （P23935） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q02366） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P42029） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q8HXG6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q95KV7） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （Q02377） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q02371） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q02376） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q02827） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q02379） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （Q02378） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q8HXG5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q02380） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q02367） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q02374） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q02365） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q02372） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （P48305） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q02369） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q02368） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q02373） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （O97725） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q05752） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） FME N-FORMYLMETHIONINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 2.80 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9W2V Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5LC5 Structure of mammalian respiratory Complex I, class2 Deposited 2016-06-19 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain B 64–210（147 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 4.35 Å
5LDW Structure of mammalian respiratory Complex I, class1 Deposited 2016-06-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain B 38–216（179 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus present in a cold room, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 4.27 Å
5LDX Structure of mammalian respiratory Complex I, class3. Deposited 2016-06-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain B 38–216（179 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer 20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7；pH 8
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 5.60 Å
5O31 Mitochondrial complex I in the deactive state Deposited 2017-05-23 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain B 38–216（179 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.13 Å
7DGQ Activity optimized supercomplex state1 Deposited 2020-11-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain D 38–216（179 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 CU COPPER (II) ION × 3 HEA HEME-A × 2 MG MAGNESIUM ION × 1 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 UQ2 UBIQUINONE-2 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.00 Å
7DGR Activity optimized supercomplex state2 Deposited 2020-11-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain D 38–216（179 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 3 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.60 Å
7DGS Activity optimized supercomplex state3 Deposited 2020-11-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain D 38–216（179 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 7.80 Å
7DGZ Activity optimized complex I (closed form) Deposited 2020-11-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain D 38–216（179 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
7DH0 Activity optimized complex I (open form) Deposited 2020-11-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain D 38–216（179 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
7DKF Activity optimized supercomplex state4 Deposited 2020-11-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain D2 38–216（179 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 8.30 Å
7QSD Bovine complex I in the active state at 3.1 A Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain B 1–216（216 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 4 LMT DODECYL-BETA-D-MALTOSIDE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7QSK Bovine complex I in lipid nanodisc, Active-Q10 Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain B 1–216（216 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.84 Å
7QSL Bovine complex I in lipid nanodisc, Active-apo Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain B 1–216（216 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.76 Å
7QSM Bovine complex I in lipid nanodisc, Deactive-ligand (composite) Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain B 1–216（216 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 GOL GLYCEROL × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.30 Å
7QSN Bovine complex I in lipid nanodisc, Deactive-apo Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain B 1–216（216 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.81 Å
7QSO Bovine complex I in lipid nanodisc, State 3 (Slack) Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain B 1–216（216 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 6 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 2 CDL CARDIOLIPIN × 6 U10 UBIQUINONE-10 × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.02 Å
7R41 Bovine complex I in the presence of IM1761092, active class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain B 1–216（216 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R42 Bovine complex I in the presence of IM1761092, active class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain B 1–216（216 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R43 Bovine complex I in the presence of IM1761092, active class iii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain B 1–216（216 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 CDL CARDIOLIPIN × 5 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R44 Bovine complex I in the presence of IM1761092, active class iv (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain B 1–216（216 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R45 Bovine complex I in the presence of IM1761092, deactive class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain B 1–216（216 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R46 Bovine complex I in the presence of IM1761092, deactive class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain B 1–216（216 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 11 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R47 Bovine complex I in the presence of IM1761092, deactive class iii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain B 1–216（216 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R48 Bovine complex I in the presence of IM1761092, deactive class iv (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain B 1–216（216 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4C Bovine complex I in the presence of IM1761092, deactive class v (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain B 1–216（216 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4D Bovine complex I in the presence of IM1761092, deactive class vi (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain B 1–216（216 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4F Bovine complex I in the presence of IM1761092, slack class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain B 1–216（216 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 7 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 CDL CARDIOLIPIN × 6 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R4G Bovine complex I in the presence of IM1761092, slack class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain B 1–216（216 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 10 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8Q0A Inward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain B 1–216（216 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 8 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0F Inward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain B 1–216（216 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 21 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0J Inward-facing, slack proteoliposome complex I at 3.8 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain B 1–216（216 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.80 Å
8Q0M Outward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain B 1–216（216 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0O Outward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain B 1–216（216 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0Q Outward-facing, slack proteoliposome complex I at 3.6 A. Initially purified in DDM Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain B 1–216（216 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.60 Å
8Q1P Inward-facing, open2 proteoliposome complex I at 2.9 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain B 1–216（216 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q1U Inward-facing, open1 proteoliposome complex I at 3.3 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain B 1–216（216 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.30 Å
8Q1Y Outward-facing, open2 proteoliposome complex I at 2.6 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain B 1–216（216 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q25 Outward-facing, open1 proteoliposome complex I at 2.8 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain B 1–216（216 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.80 Å
8Q45 Inward-facing, closed proteoliposome complex I at 2.7 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain B 1–216（216 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.70 Å
8Q46 Inward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain B 1–216（216 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q47 Inward-facing, open1 proteoliposome complex I at 2.9 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain B 1–216（216 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 26 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q48 Outward-facing, closed proteoliposome complex I at 2.5 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain B 1–216（216 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 35 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.50 Å
8Q49 Outward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain B 1–216（216 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 FVH [[(2~{R},3~{S},4~{R},5~{R})-5-[(3~{R})-3-aminocarbonylpiperidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2~{R},3~{R},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3-oxidanyl-4-phosphonooxy-oxolan-2-yl]methyl hydrogen phosphate × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q4A Outward-facing, open1 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain B 1–216（216 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
9SMF Reduced bovine complex I in lipid nanodisc, NADH-active-Q10 Deposited 2025-09-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain B 1–216（216 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.51 Å
9SMG Reduced bovine complex I in lipid nanodisc, NADH-active-altQ10 Deposited 2025-09-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain B 1–216（216 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.42 Å
9SMH Reduced bovine complex I in lipid nanodisc, NADH-active-DDM Deposited 2025-09-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain B 1–216（216 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.61 Å
9SMI Reduced bovine complex I in lipid nanodisc, NADH-deactive Deposited 2025-09-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain B 1–216（216 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 27 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 LMT DODECYL-BETA-D-MALTOSIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 CHD CHOLIC ACID × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.01 Å
9W2U Cryo-EM structure of complex I on the bovine heart submitochondrial particles, open Deposited 2025-07-28 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain B 62–216（155 aa）
Not recorded FME N-FORMYLMETHIONINE × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å

49 other PDB entries and 49 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUS7_BOVIN
Isoform —
PDB entities 2
Chains and sequence ranges Author chain B； PDBConstruct 1–155； UniProt 62–216

NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial

Bos taurus

UniProt P23709

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain C； UniProt 44–252 Not recorded NADH-ubiquinone oxidoreductase chain 3 × 1 （Q8WAB4） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （P42026） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （P17694） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P04394） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P25708） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P15690） NADH-ubiquinone oxidoreductase chain 1 × 1 （Q6EMT1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （P42028） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03924） NADH-ubiquinone oxidoreductase chain 4L × 1 （Q6EMS3） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03920） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03910） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03892） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （P34942） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （P34943） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q02375） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P23934） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q02370） Acyl carrier protein, mitochondrial × 1 （Q9CR21） Acyl carrier protein, mitochondrial × 1 （P52505） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （P23935） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q02366） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P42029） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q8HXG6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q95KV7） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （Q02377） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q02371） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q02376） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q02827） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q02379） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （Q02378） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q8HXG5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q02380） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q02367） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q02374） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q02365） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q02372） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （P48305） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q02369） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q02368） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q02373） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （O97725） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q05752） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） FME N-FORMYLMETHIONINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 2.80 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9W2V Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5LC5 Structure of mammalian respiratory Complex I, class2 Deposited 2016-06-19 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain C 46–251（206 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 4.35 Å
5LDW Structure of mammalian respiratory Complex I, class1 Deposited 2016-06-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain C 39–266（228 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus present in a cold room, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 4.27 Å
5LDX Structure of mammalian respiratory Complex I, class3. Deposited 2016-06-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain C 39–266（228 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer 20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7；pH 8
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 5.60 Å
5O31 Mitochondrial complex I in the deactive state Deposited 2017-05-23 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain C 39–266（228 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.13 Å
7DGQ Activity optimized supercomplex state1 Deposited 2020-11-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain C 39–266（228 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 CU COPPER (II) ION × 3 HEA HEME-A × 2 MG MAGNESIUM ION × 1 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 UQ2 UBIQUINONE-2 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.00 Å
7DGR Activity optimized supercomplex state2 Deposited 2020-11-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain C 39–266（228 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 3 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.60 Å
7DGS Activity optimized supercomplex state3 Deposited 2020-11-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain C 39–266（228 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 7.80 Å
7DGZ Activity optimized complex I (closed form) Deposited 2020-11-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain C 39–266（228 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
7DH0 Activity optimized complex I (open form) Deposited 2020-11-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain C 39–266（228 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
7DKF Activity optimized supercomplex state4 Deposited 2020-11-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain C2 39–266（228 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 8.30 Å
7QSD Bovine complex I in the active state at 3.1 A Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain C 1–266（266 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 4 LMT DODECYL-BETA-D-MALTOSIDE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7QSK Bovine complex I in lipid nanodisc, Active-Q10 Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain C 1–266（266 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.84 Å
7QSL Bovine complex I in lipid nanodisc, Active-apo Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain C 1–266（266 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.76 Å
7QSM Bovine complex I in lipid nanodisc, Deactive-ligand (composite) Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain C 1–266（266 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 GOL GLYCEROL × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.30 Å
7QSN Bovine complex I in lipid nanodisc, Deactive-apo Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain C 1–266（266 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.81 Å
7QSO Bovine complex I in lipid nanodisc, State 3 (Slack) Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain C 1–266（266 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 6 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 2 CDL CARDIOLIPIN × 6 U10 UBIQUINONE-10 × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.02 Å
7R41 Bovine complex I in the presence of IM1761092, active class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain C 1–266（266 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R42 Bovine complex I in the presence of IM1761092, active class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain C 1–266（266 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R43 Bovine complex I in the presence of IM1761092, active class iii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain C 1–266（266 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 CDL CARDIOLIPIN × 5 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R44 Bovine complex I in the presence of IM1761092, active class iv (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain C 1–266（266 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R45 Bovine complex I in the presence of IM1761092, deactive class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain C 1–266（266 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R46 Bovine complex I in the presence of IM1761092, deactive class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain C 1–266（266 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 11 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R47 Bovine complex I in the presence of IM1761092, deactive class iii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain C 1–266（266 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R48 Bovine complex I in the presence of IM1761092, deactive class iv (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain C 1–266（266 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4C Bovine complex I in the presence of IM1761092, deactive class v (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain C 1–266（266 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4D Bovine complex I in the presence of IM1761092, deactive class vi (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain C 1–266（266 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4F Bovine complex I in the presence of IM1761092, slack class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain C 1–266（266 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 7 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 CDL CARDIOLIPIN × 6 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R4G Bovine complex I in the presence of IM1761092, slack class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain C 1–266（266 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 10 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8Q0A Inward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain C 1–266（266 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 8 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0F Inward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain C 1–266（266 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 21 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0J Inward-facing, slack proteoliposome complex I at 3.8 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain C 1–266（266 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.80 Å
8Q0M Outward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain C 1–266（266 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0O Outward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain C 1–266（266 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0Q Outward-facing, slack proteoliposome complex I at 3.6 A. Initially purified in DDM Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain C 1–266（266 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.60 Å
8Q1P Inward-facing, open2 proteoliposome complex I at 2.9 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain C 1–266（266 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q1U Inward-facing, open1 proteoliposome complex I at 3.3 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain C 1–266（266 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.30 Å
8Q1Y Outward-facing, open2 proteoliposome complex I at 2.6 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain C 1–266（266 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q25 Outward-facing, open1 proteoliposome complex I at 2.8 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain C 1–266（266 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.80 Å
8Q45 Inward-facing, closed proteoliposome complex I at 2.7 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain C 1–266（266 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.70 Å
8Q46 Inward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain C 1–266（266 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q47 Inward-facing, open1 proteoliposome complex I at 2.9 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain C 1–266（266 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 26 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q48 Outward-facing, closed proteoliposome complex I at 2.5 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain C 1–266（266 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 35 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.50 Å
8Q49 Outward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain C 1–266（266 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 FVH [[(2~{R},3~{S},4~{R},5~{R})-5-[(3~{R})-3-aminocarbonylpiperidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2~{R},3~{R},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3-oxidanyl-4-phosphonooxy-oxolan-2-yl]methyl hydrogen phosphate × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q4A Outward-facing, open1 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain C 1–266（266 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
9SMF Reduced bovine complex I in lipid nanodisc, NADH-active-Q10 Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain C 1–266（266 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.51 Å
9SMG Reduced bovine complex I in lipid nanodisc, NADH-active-altQ10 Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain C 1–266（266 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.42 Å
9SMH Reduced bovine complex I in lipid nanodisc, NADH-active-DDM Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain C 1–266（266 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.61 Å
9SMI Reduced bovine complex I in lipid nanodisc, NADH-deactive Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain C 1–266（266 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 27 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 LMT DODECYL-BETA-D-MALTOSIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 CHD CHOLIC ACID × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.01 Å
9W2U Cryo-EM structure of complex I on the bovine heart submitochondrial particles, open Deposited 2025-07-28 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain C 44–252（209 aa）
Not recorded FME N-FORMYLMETHIONINE × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å

49 other PDB entries and 49 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUS3_BOVIN
Isoform —
PDB entities 3
Chains and sequence ranges Author chain C； PDBConstruct 1–209； UniProt 44–252

NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial

Bos taurus

UniProt P17694

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain D； UniProt 34–463 Non-standard monomer：Yes (specific site not provided by mmCIF) NADH-ubiquinone oxidoreductase chain 3 × 1 （Q8WAB4） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （P42026） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （P23709） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P04394） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P25708） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P15690） NADH-ubiquinone oxidoreductase chain 1 × 1 （Q6EMT1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （P42028） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03924） NADH-ubiquinone oxidoreductase chain 4L × 1 （Q6EMS3） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03920） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03910） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03892） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （P34942） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （P34943） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q02375） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P23934） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q02370） Acyl carrier protein, mitochondrial × 1 （Q9CR21） Acyl carrier protein, mitochondrial × 1 （P52505） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （P23935） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q02366） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P42029） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q8HXG6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q95KV7） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （Q02377） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q02371） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q02376） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q02827） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q02379） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （Q02378） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q8HXG5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q02380） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q02367） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q02374） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q02365） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q02372） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （P48305） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q02369） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q02368） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q02373） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （O97725） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q05752） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） FME N-FORMYLMETHIONINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 2.80 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9W2V Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5LC5 Structure of mammalian respiratory Complex I, class2 Deposited 2016-06-19 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain D 38–463（426 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 4.35 Å
5LDW Structure of mammalian respiratory Complex I, class1 Deposited 2016-06-28 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain D 35–463（429 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus present in a cold room, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 4.27 Å
5LDX Structure of mammalian respiratory Complex I, class3. Deposited 2016-06-28 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain D 35–463（429 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer 20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7；pH 8
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 5.60 Å
5O31 Mitochondrial complex I in the deactive state Deposited 2017-05-23 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain D 34–463（430 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.13 Å
7DGQ Activity optimized supercomplex state1 Deposited 2020-11-12 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain B 34–463（430 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 CU COPPER (II) ION × 3 HEA HEME-A × 2 MG MAGNESIUM ION × 1 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 UQ2 UBIQUINONE-2 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.00 Å
7DGR Activity optimized supercomplex state2 Deposited 2020-11-12 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain B 34–463（430 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 3 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.60 Å
7DGS Activity optimized supercomplex state3 Deposited 2020-11-12 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain B 34–463（430 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 7.80 Å
7DGZ Activity optimized complex I (closed form) Deposited 2020-11-12 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain B 34–463（430 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
7DH0 Activity optimized complex I (open form) Deposited 2020-11-12 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain B 34–463（430 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
7DKF Activity optimized supercomplex state4 Deposited 2020-11-24 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain B2 34–463（430 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 8.30 Å
7QSD Bovine complex I in the active state at 3.1 A Deposited 2022-01-13 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain D 1–463（463 aa）
Mutation：Q129R (Q162R) Non-standard monomer：Yes (specific site not provided by mmCIF) PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 4 LMT DODECYL-BETA-D-MALTOSIDE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7QSK Bovine complex I in lipid nanodisc, Active-Q10 Deposited 2022-01-13 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain D 1–463（463 aa）
Mutation：Q129R (Q162R) Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.84 Å
7QSL Bovine complex I in lipid nanodisc, Active-apo Deposited 2022-01-13 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain D 1–463（463 aa）
Mutation：Q129R (Q162R) Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.76 Å
7QSM Bovine complex I in lipid nanodisc, Deactive-ligand (composite) Deposited 2022-01-13 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain D 1–463（463 aa）
Mutation：Q129R (Q162R) Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 GOL GLYCEROL × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.30 Å
7QSN Bovine complex I in lipid nanodisc, Deactive-apo Deposited 2022-01-13 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain D 1–463（463 aa）
Mutation：Q129R (Q162R) Non-standard monomer：Yes (specific site not provided by mmCIF) PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.81 Å
7QSO Bovine complex I in lipid nanodisc, State 3 (Slack) Deposited 2022-01-13 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain D 1–463（463 aa）
Mutation：Q129R (Q162R) Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 6 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 2 CDL CARDIOLIPIN × 6 U10 UBIQUINONE-10 × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.02 Å
7R41 Bovine complex I in the presence of IM1761092, active class i (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain D 1–463（463 aa）
Mutation：Q166R Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R42 Bovine complex I in the presence of IM1761092, active class ii (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain D 1–463（463 aa）
Mutation：Q166R Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R43 Bovine complex I in the presence of IM1761092, active class iii (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain D 1–463（463 aa）
Mutation：Q166R Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 CDL CARDIOLIPIN × 5 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R44 Bovine complex I in the presence of IM1761092, active class iv (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain D 1–463（463 aa）
Mutation：Q166R Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R45 Bovine complex I in the presence of IM1761092, deactive class i (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain D 1–463（463 aa）
Mutation：Q166R Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R46 Bovine complex I in the presence of IM1761092, deactive class ii (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain D 1–463（463 aa）
Mutation：Q166R Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 11 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R47 Bovine complex I in the presence of IM1761092, deactive class iii (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain D 1–463（463 aa）
Mutation：Q166R Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R48 Bovine complex I in the presence of IM1761092, deactive class iv (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain D 1–463（463 aa）
Mutation：Q166R Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4C Bovine complex I in the presence of IM1761092, deactive class v (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain D 1–463（463 aa）
Mutation：Q166R Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4D Bovine complex I in the presence of IM1761092, deactive class vi (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain D 1–463（463 aa）
Mutation：Q166R Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4F Bovine complex I in the presence of IM1761092, slack class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain D 1–463（463 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 7 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 CDL CARDIOLIPIN × 6 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R4G Bovine complex I in the presence of IM1761092, slack class ii (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain D 1–463（463 aa）
Mutation：Q166R Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 10 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8Q0A Inward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain D 1–463（463 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 8 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0F Inward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain D 1–463（463 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 21 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0J Inward-facing, slack proteoliposome complex I at 3.8 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain D 1–463（463 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.80 Å
8Q0M Outward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain D 1–463（463 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0O Outward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain D 1–463（463 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0Q Outward-facing, slack proteoliposome complex I at 3.6 A. Initially purified in DDM Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain D 1–463（463 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.60 Å
8Q1P Inward-facing, open2 proteoliposome complex I at 2.9 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain D 1–463（463 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q1U Inward-facing, open1 proteoliposome complex I at 3.3 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain D 1–463（463 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.30 Å
8Q1Y Outward-facing, open2 proteoliposome complex I at 2.6 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain D 1–463（463 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q25 Outward-facing, open1 proteoliposome complex I at 2.8 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain D 1–463（463 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.80 Å
8Q45 Inward-facing, closed proteoliposome complex I at 2.7 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain D 1–463（463 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.70 Å
8Q46 Inward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain D 1–463（463 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q47 Inward-facing, open1 proteoliposome complex I at 2.9 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain D 1–463（463 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 26 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q48 Outward-facing, closed proteoliposome complex I at 2.5 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain D 1–463（463 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 35 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.50 Å
8Q49 Outward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain D 1–463（463 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 FVH [[(2~{R},3~{S},4~{R},5~{R})-5-[(3~{R})-3-aminocarbonylpiperidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2~{R},3~{R},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3-oxidanyl-4-phosphonooxy-oxolan-2-yl]methyl hydrogen phosphate × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q4A Outward-facing, open1 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain D 1–463（463 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
9SMF Reduced bovine complex I in lipid nanodisc, NADH-active-Q10 Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain D 1–463（463 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.51 Å
9SMG Reduced bovine complex I in lipid nanodisc, NADH-active-altQ10 Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain D 1–463（463 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.42 Å
9SMH Reduced bovine complex I in lipid nanodisc, NADH-active-DDM Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain D 1–463（463 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.61 Å
9SMI Reduced bovine complex I in lipid nanodisc, NADH-deactive Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain D 1–463（463 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 27 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 LMT DODECYL-BETA-D-MALTOSIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 CHD CHOLIC ACID × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.01 Å
9W2U Cryo-EM structure of complex I on the bovine heart submitochondrial particles, open Deposited 2025-07-28 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain D 34–463（430 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) FME N-FORMYLMETHIONINE × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å

49 other PDB entries and 49 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUS2_BOVIN
Isoform —
PDB entities 4
Chains and sequence ranges Author chain D； PDBConstruct 1–430； UniProt 34–463

NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial

Bos taurus

UniProt P04394

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain E； UniProt 36–249 Not recorded NADH-ubiquinone oxidoreductase chain 3 × 1 （Q8WAB4） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （P42026） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （P23709） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （P17694） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P25708） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P15690） NADH-ubiquinone oxidoreductase chain 1 × 1 （Q6EMT1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （P42028） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03924） NADH-ubiquinone oxidoreductase chain 4L × 1 （Q6EMS3） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03920） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03910） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03892） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （P34942） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （P34943） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q02375） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P23934） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q02370） Acyl carrier protein, mitochondrial × 1 （Q9CR21） Acyl carrier protein, mitochondrial × 1 （P52505） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （P23935） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q02366） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P42029） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q8HXG6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q95KV7） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （Q02377） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q02371） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q02376） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q02827） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q02379） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （Q02378） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q8HXG5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q02380） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q02367） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q02374） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q02365） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q02372） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （P48305） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q02369） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q02368） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q02373） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （O97725） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q05752） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） FME N-FORMYLMETHIONINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 2.80 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9W2V Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5LC5 Structure of mammalian respiratory Complex I, class2 Deposited 2016-06-19 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain E 1–249（249 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 4.35 Å
5LDW Structure of mammalian respiratory Complex I, class1 Deposited 2016-06-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain E 33–249（217 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus present in a cold room, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 4.27 Å
5LDX Structure of mammalian respiratory Complex I, class3. Deposited 2016-06-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain E 33–249（217 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer 20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7；pH 8
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 5.60 Å
5O31 Mitochondrial complex I in the deactive state Deposited 2017-05-23 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain E 33–249（217 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.13 Å
7DGQ Activity optimized supercomplex state1 Deposited 2020-11-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain 9 33–249（217 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 CU COPPER (II) ION × 3 HEA HEME-A × 2 MG MAGNESIUM ION × 1 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 UQ2 UBIQUINONE-2 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.00 Å
7DGR Activity optimized supercomplex state2 Deposited 2020-11-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain 9 33–249（217 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 3 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.60 Å
7DGS Activity optimized supercomplex state3 Deposited 2020-11-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain 9 33–249（217 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 7.80 Å
7DGZ Activity optimized complex I (closed form) Deposited 2020-11-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain 9 33–249（217 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
7DH0 Activity optimized complex I (open form) Deposited 2020-11-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain 9 33–249（217 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
7DKF Activity optimized supercomplex state4 Deposited 2020-11-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain 92 33–249（217 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 8.30 Å
7QSD Bovine complex I in the active state at 3.1 A Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain E 1–249（249 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 4 LMT DODECYL-BETA-D-MALTOSIDE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7QSK Bovine complex I in lipid nanodisc, Active-Q10 Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain E 1–249（249 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.84 Å
7QSL Bovine complex I in lipid nanodisc, Active-apo Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain E 1–249（249 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.76 Å
7QSM Bovine complex I in lipid nanodisc, Deactive-ligand (composite) Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain E 1–249（249 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 GOL GLYCEROL × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.30 Å
7QSN Bovine complex I in lipid nanodisc, Deactive-apo Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain E 1–249（249 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.81 Å
7QSO Bovine complex I in lipid nanodisc, State 3 (Slack) Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain E 1–249（249 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 6 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 2 CDL CARDIOLIPIN × 6 U10 UBIQUINONE-10 × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.02 Å
7R41 Bovine complex I in the presence of IM1761092, active class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain E 1–249（249 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R42 Bovine complex I in the presence of IM1761092, active class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain E 1–249（249 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R43 Bovine complex I in the presence of IM1761092, active class iii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain E 1–249（249 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 CDL CARDIOLIPIN × 5 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R44 Bovine complex I in the presence of IM1761092, active class iv (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain E 1–249（249 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R45 Bovine complex I in the presence of IM1761092, deactive class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain E 1–249（249 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R46 Bovine complex I in the presence of IM1761092, deactive class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain E 1–249（249 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 11 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R47 Bovine complex I in the presence of IM1761092, deactive class iii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain E 1–249（249 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R48 Bovine complex I in the presence of IM1761092, deactive class iv (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain E 1–249（249 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4C Bovine complex I in the presence of IM1761092, deactive class v (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain E 1–249（249 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4D Bovine complex I in the presence of IM1761092, deactive class vi (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain E 1–249（249 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4F Bovine complex I in the presence of IM1761092, slack class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain E 1–249（249 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 7 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 CDL CARDIOLIPIN × 6 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R4G Bovine complex I in the presence of IM1761092, slack class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain E 1–249（249 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 10 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8Q0A Inward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain E 1–249（249 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 8 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0F Inward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain E 1–249（249 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 21 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0J Inward-facing, slack proteoliposome complex I at 3.8 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain E 1–249（249 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.80 Å
8Q0M Outward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain E 1–249（249 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0O Outward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain E 1–249（249 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0Q Outward-facing, slack proteoliposome complex I at 3.6 A. Initially purified in DDM Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain E 1–249（249 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.60 Å
8Q1P Inward-facing, open2 proteoliposome complex I at 2.9 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain E 1–249（249 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q1U Inward-facing, open1 proteoliposome complex I at 3.3 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain E 1–249（249 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.30 Å
8Q1Y Outward-facing, open2 proteoliposome complex I at 2.6 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain E 1–249（249 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q25 Outward-facing, open1 proteoliposome complex I at 2.8 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain E 1–249（249 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.80 Å
8Q45 Inward-facing, closed proteoliposome complex I at 2.7 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain E 1–249（249 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.70 Å
8Q46 Inward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain E 1–249（249 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q47 Inward-facing, open1 proteoliposome complex I at 2.9 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain E 1–249（249 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 26 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q48 Outward-facing, closed proteoliposome complex I at 2.5 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain E 1–249（249 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 35 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.50 Å
8Q49 Outward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain E 1–249（249 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 FVH [[(2~{R},3~{S},4~{R},5~{R})-5-[(3~{R})-3-aminocarbonylpiperidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2~{R},3~{R},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3-oxidanyl-4-phosphonooxy-oxolan-2-yl]methyl hydrogen phosphate × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q4A Outward-facing, open1 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain E 1–249（249 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
9SMF Reduced bovine complex I in lipid nanodisc, NADH-active-Q10 Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain E 1–249（249 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.51 Å
9SMG Reduced bovine complex I in lipid nanodisc, NADH-active-altQ10 Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain E 1–249（249 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.42 Å
9SMH Reduced bovine complex I in lipid nanodisc, NADH-active-DDM Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain E 1–249（249 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.61 Å
9SMI Reduced bovine complex I in lipid nanodisc, NADH-deactive Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain E 1–249（249 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 27 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 LMT DODECYL-BETA-D-MALTOSIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 CHD CHOLIC ACID × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.01 Å
9W2U Cryo-EM structure of complex I on the bovine heart submitochondrial particles, open Deposited 2025-07-28 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain E 36–249（214 aa）
Not recorded FME N-FORMYLMETHIONINE × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å

49 other PDB entries and 49 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUV2_BOVIN
Isoform —
PDB entities 5
Chains and sequence ranges Author chain E； PDBConstruct 1–214； UniProt 36–249

NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial

Bos taurus

UniProt P25708

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain F； UniProt 27–458 Not recorded NADH-ubiquinone oxidoreductase chain 3 × 1 （Q8WAB4） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （P42026） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （P23709） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （P17694） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P04394） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P15690） NADH-ubiquinone oxidoreductase chain 1 × 1 （Q6EMT1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （P42028） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03924） NADH-ubiquinone oxidoreductase chain 4L × 1 （Q6EMS3） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03920） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03910） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03892） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （P34942） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （P34943） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q02375） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P23934） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q02370） Acyl carrier protein, mitochondrial × 1 （Q9CR21） Acyl carrier protein, mitochondrial × 1 （P52505） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （P23935） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q02366） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P42029） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q8HXG6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q95KV7） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （Q02377） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q02371） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q02376） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q02827） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q02379） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （Q02378） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q8HXG5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q02380） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q02367） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q02374） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q02365） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q02372） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （P48305） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q02369） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q02368） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q02373） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （O97725） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q05752） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） FME N-FORMYLMETHIONINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 2.80 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9W2V Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5LC5 Structure of mammalian respiratory Complex I, class2 Deposited 2016-06-19 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain F 1–464（464 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 4.35 Å
5LDW Structure of mammalian respiratory Complex I, class1 Deposited 2016-06-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain F 21–464（444 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus present in a cold room, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 4.27 Å
5LDX Structure of mammalian respiratory Complex I, class3. Deposited 2016-06-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain F 21–464（444 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer 20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7；pH 8
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 5.60 Å
5O31 Mitochondrial complex I in the deactive state Deposited 2017-05-23 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain F 1–464（464 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.13 Å
7DGQ Activity optimized supercomplex state1 Deposited 2020-11-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain 8 21–464（444 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 CU COPPER (II) ION × 3 HEA HEME-A × 2 MG MAGNESIUM ION × 1 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 UQ2 UBIQUINONE-2 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.00 Å
7DGR Activity optimized supercomplex state2 Deposited 2020-11-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain 8 21–464（444 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 3 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.60 Å
7DGS Activity optimized supercomplex state3 Deposited 2020-11-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain 8 21–464（444 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 7.80 Å
7DGZ Activity optimized complex I (closed form) Deposited 2020-11-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain 8 21–464（444 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
7DH0 Activity optimized complex I (open form) Deposited 2020-11-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain 8 21–464（444 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
7DKF Activity optimized supercomplex state4 Deposited 2020-11-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain 82 21–464（444 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 8.30 Å
7QSD Bovine complex I in the active state at 3.1 A Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain F 1–464（464 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 4 LMT DODECYL-BETA-D-MALTOSIDE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7QSK Bovine complex I in lipid nanodisc, Active-Q10 Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain F 1–464（464 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.84 Å
7QSL Bovine complex I in lipid nanodisc, Active-apo Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain F 1–464（464 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.76 Å
7QSM Bovine complex I in lipid nanodisc, Deactive-ligand (composite) Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain F 1–464（464 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 GOL GLYCEROL × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.30 Å
7QSN Bovine complex I in lipid nanodisc, Deactive-apo Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain F 1–464（464 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.81 Å
7QSO Bovine complex I in lipid nanodisc, State 3 (Slack) Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain F 1–464（464 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 6 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 2 CDL CARDIOLIPIN × 6 U10 UBIQUINONE-10 × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.02 Å
7R41 Bovine complex I in the presence of IM1761092, active class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain F 1–464（464 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R42 Bovine complex I in the presence of IM1761092, active class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain F 1–464（464 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R43 Bovine complex I in the presence of IM1761092, active class iii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain F 1–464（464 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 CDL CARDIOLIPIN × 5 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R44 Bovine complex I in the presence of IM1761092, active class iv (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain F 1–464（464 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R45 Bovine complex I in the presence of IM1761092, deactive class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain F 1–464（464 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R46 Bovine complex I in the presence of IM1761092, deactive class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain F 1–464（464 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 11 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R47 Bovine complex I in the presence of IM1761092, deactive class iii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain F 1–464（464 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R48 Bovine complex I in the presence of IM1761092, deactive class iv (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain F 1–464（464 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4C Bovine complex I in the presence of IM1761092, deactive class v (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain F 1–464（464 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4D Bovine complex I in the presence of IM1761092, deactive class vi (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain F 1–464（464 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4F Bovine complex I in the presence of IM1761092, slack class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain F 1–464（464 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 7 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 CDL CARDIOLIPIN × 6 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R4G Bovine complex I in the presence of IM1761092, slack class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain F 1–464（464 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 10 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8Q0A Inward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain F 1–464（464 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 8 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0F Inward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain F 1–464（464 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 21 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0J Inward-facing, slack proteoliposome complex I at 3.8 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain F 1–464（464 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.80 Å
8Q0M Outward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain F 1–464（464 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0O Outward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain F 1–464（464 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0Q Outward-facing, slack proteoliposome complex I at 3.6 A. Initially purified in DDM Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain F 1–464（464 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.60 Å
8Q1P Inward-facing, open2 proteoliposome complex I at 2.9 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain F 1–464（464 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q1U Inward-facing, open1 proteoliposome complex I at 3.3 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain F 1–464（464 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.30 Å
8Q1Y Outward-facing, open2 proteoliposome complex I at 2.6 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain F 1–464（464 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q25 Outward-facing, open1 proteoliposome complex I at 2.8 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain F 1–464（464 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.80 Å
8Q45 Inward-facing, closed proteoliposome complex I at 2.7 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain F 1–464（464 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.70 Å
8Q46 Inward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain F 1–464（464 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q47 Inward-facing, open1 proteoliposome complex I at 2.9 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain F 1–464（464 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 26 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q48 Outward-facing, closed proteoliposome complex I at 2.5 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain F 1–464（464 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 35 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.50 Å
8Q49 Outward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain F 1–464（464 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 FVH [[(2~{R},3~{S},4~{R},5~{R})-5-[(3~{R})-3-aminocarbonylpiperidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2~{R},3~{R},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3-oxidanyl-4-phosphonooxy-oxolan-2-yl]methyl hydrogen phosphate × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q4A Outward-facing, open1 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain F 1–464（464 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
9SMF Reduced bovine complex I in lipid nanodisc, NADH-active-Q10 Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain F 1–464（464 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.51 Å
9SMG Reduced bovine complex I in lipid nanodisc, NADH-active-altQ10 Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain F 1–464（464 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.42 Å
9SMH Reduced bovine complex I in lipid nanodisc, NADH-active-DDM Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain F 1–464（464 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.61 Å
9SMI Reduced bovine complex I in lipid nanodisc, NADH-deactive Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain F 1–464（464 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 27 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 LMT DODECYL-BETA-D-MALTOSIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 CHD CHOLIC ACID × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.01 Å
9W2U Cryo-EM structure of complex I on the bovine heart submitochondrial particles, open Deposited 2025-07-28 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain F 27–458（432 aa）
Not recorded FME N-FORMYLMETHIONINE × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å

49 other PDB entries and 49 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUV1_BOVIN
Isoform —
PDB entities 6
Chains and sequence ranges Author chain F； PDBConstruct 1–432； UniProt 27–458

NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial

Bos taurus

UniProt P15690

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain G； UniProt 29–727 Not recorded NADH-ubiquinone oxidoreductase chain 3 × 1 （Q8WAB4） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （P42026） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （P23709） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （P17694） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P04394） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P25708） NADH-ubiquinone oxidoreductase chain 1 × 1 （Q6EMT1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （P42028） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03924） NADH-ubiquinone oxidoreductase chain 4L × 1 （Q6EMS3） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03920） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03910） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03892） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （P34942） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （P34943） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q02375） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P23934） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q02370） Acyl carrier protein, mitochondrial × 1 （Q9CR21） Acyl carrier protein, mitochondrial × 1 （P52505） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （P23935） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q02366） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P42029） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q8HXG6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q95KV7） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （Q02377） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q02371） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q02376） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q02827） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q02379） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （Q02378） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q8HXG5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q02380） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q02367） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q02374） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q02365） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q02372） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （P48305） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q02369） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q02368） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q02373） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （O97725） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q05752） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） FME N-FORMYLMETHIONINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 2.80 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9W2V Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5LC5 Structure of mammalian respiratory Complex I, class2 Deposited 2016-06-19 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain G 1–232（232 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 4.35 Å
5LDW Structure of mammalian respiratory Complex I, class1 Deposited 2016-06-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain G 31–232（202 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus present in a cold room, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 4.27 Å
5LDX Structure of mammalian respiratory Complex I, class3. Deposited 2016-06-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain G 31–232（202 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer 20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7；pH 8
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 5.60 Å
5O31 Mitochondrial complex I in the deactive state Deposited 2017-05-23 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain G 24–727（704 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.13 Å
7DGQ Activity optimized supercomplex state1 Deposited 2020-11-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain A 24–727（704 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 CU COPPER (II) ION × 3 HEA HEME-A × 2 MG MAGNESIUM ION × 1 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 UQ2 UBIQUINONE-2 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.00 Å
7DGR Activity optimized supercomplex state2 Deposited 2020-11-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain A 24–727（704 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 3 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.60 Å
7DGS Activity optimized supercomplex state3 Deposited 2020-11-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain A 24–727（704 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 7.80 Å
7DGZ Activity optimized complex I (closed form) Deposited 2020-11-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain A 24–727（704 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
7DH0 Activity optimized complex I (open form) Deposited 2020-11-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain A 24–727（704 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
7DKF Activity optimized supercomplex state4 Deposited 2020-11-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain A2 24–727（704 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 8.30 Å
7QSD Bovine complex I in the active state at 3.1 A Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain G 1–727（727 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 4 LMT DODECYL-BETA-D-MALTOSIDE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7QSK Bovine complex I in lipid nanodisc, Active-Q10 Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain G 1–727（727 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.84 Å
7QSL Bovine complex I in lipid nanodisc, Active-apo Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain G 1–727（727 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.76 Å
7QSM Bovine complex I in lipid nanodisc, Deactive-ligand (composite) Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain G 1–727（727 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 GOL GLYCEROL × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.30 Å
7QSN Bovine complex I in lipid nanodisc, Deactive-apo Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain G 1–727（727 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.81 Å
7QSO Bovine complex I in lipid nanodisc, State 3 (Slack) Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain G 1–727（727 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 6 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 2 CDL CARDIOLIPIN × 6 U10 UBIQUINONE-10 × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.02 Å
7R41 Bovine complex I in the presence of IM1761092, active class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain G 1–727（727 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R42 Bovine complex I in the presence of IM1761092, active class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain G 1–727（727 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R43 Bovine complex I in the presence of IM1761092, active class iii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain G 1–727（727 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 CDL CARDIOLIPIN × 5 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R44 Bovine complex I in the presence of IM1761092, active class iv (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain G 1–727（727 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R45 Bovine complex I in the presence of IM1761092, deactive class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain G 1–727（727 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R46 Bovine complex I in the presence of IM1761092, deactive class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain G 1–727（727 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 11 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R47 Bovine complex I in the presence of IM1761092, deactive class iii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain G 1–727（727 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R48 Bovine complex I in the presence of IM1761092, deactive class iv (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain G 1–727（727 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4C Bovine complex I in the presence of IM1761092, deactive class v (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain G 1–727（727 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4D Bovine complex I in the presence of IM1761092, deactive class vi (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain G 1–727（727 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4F Bovine complex I in the presence of IM1761092, slack class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain G 1–727（727 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 7 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 CDL CARDIOLIPIN × 6 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R4G Bovine complex I in the presence of IM1761092, slack class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain G 1–727（727 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 10 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8Q0A Inward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain G 1–727（727 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 8 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0F Inward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain G 1–727（727 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 21 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0J Inward-facing, slack proteoliposome complex I at 3.8 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain G 1–727（727 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.80 Å
8Q0M Outward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain G 1–727（727 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0O Outward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain G 1–727（727 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0Q Outward-facing, slack proteoliposome complex I at 3.6 A. Initially purified in DDM Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain G 1–727（727 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.60 Å
8Q1P Inward-facing, open2 proteoliposome complex I at 2.9 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain G 1–727（727 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q1U Inward-facing, open1 proteoliposome complex I at 3.3 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain G 1–727（727 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.30 Å
8Q1Y Outward-facing, open2 proteoliposome complex I at 2.6 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain G 1–727（727 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q25 Outward-facing, open1 proteoliposome complex I at 2.8 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain G 1–727（727 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.80 Å
8Q45 Inward-facing, closed proteoliposome complex I at 2.7 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain G 1–727（727 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.70 Å
8Q46 Inward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain G 1–727（727 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q47 Inward-facing, open1 proteoliposome complex I at 2.9 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain G 1–727（727 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 26 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q48 Outward-facing, closed proteoliposome complex I at 2.5 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain G 1–727（727 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 35 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.50 Å
8Q49 Outward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain G 1–727（727 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 FVH [[(2~{R},3~{S},4~{R},5~{R})-5-[(3~{R})-3-aminocarbonylpiperidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2~{R},3~{R},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3-oxidanyl-4-phosphonooxy-oxolan-2-yl]methyl hydrogen phosphate × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q4A Outward-facing, open1 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain G 1–727（727 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
9SMF Reduced bovine complex I in lipid nanodisc, NADH-active-Q10 Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain G 1–727（727 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.51 Å
9SMG Reduced bovine complex I in lipid nanodisc, NADH-active-altQ10 Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain G 1–727（727 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.42 Å
9SMH Reduced bovine complex I in lipid nanodisc, NADH-active-DDM Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain G 1–727（727 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.61 Å
9SMI Reduced bovine complex I in lipid nanodisc, NADH-deactive Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain G 1–727（727 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 27 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 LMT DODECYL-BETA-D-MALTOSIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 CHD CHOLIC ACID × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.01 Å
9W2U Cryo-EM structure of complex I on the bovine heart submitochondrial particles, open Deposited 2025-07-28 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain G 29–727（699 aa）
Not recorded FME N-FORMYLMETHIONINE × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å

49 other PDB entries and 49 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUS1_BOVIN
Isoform —
PDB entities 7
Chains and sequence ranges Author chain G； PDBConstruct 1–699； UniProt 29–727

NADH-ubiquinone oxidoreductase chain 1

Bos taurus

UniProt Q6EMT1

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain H； UniProt 1–318 Non-standard monomer：Yes (specific site not provided by mmCIF) NADH-ubiquinone oxidoreductase chain 3 × 1 （Q8WAB4） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （P42026） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （P23709） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （P17694） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P04394） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P25708） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P15690） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （P42028） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03924） NADH-ubiquinone oxidoreductase chain 4L × 1 （Q6EMS3） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03920） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03910） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03892） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （P34942） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （P34943） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q02375） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P23934） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q02370） Acyl carrier protein, mitochondrial × 1 （Q9CR21） Acyl carrier protein, mitochondrial × 1 （P52505） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （P23935） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q02366） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P42029） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q8HXG6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q95KV7） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （Q02377） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q02371） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q02376） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q02827） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q02379） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （Q02378） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q8HXG5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q02380） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q02367） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q02374） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q02365） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q02372） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （P48305） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q02369） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q02368） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q02373） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （O97725） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q05752） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） FME N-FORMYLMETHIONINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 2.80 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

No other PDB entry for the same UniProt protein was found.

View Construct and Data Evidence
UniProt name NU1M_BOSIN
Isoform —
PDB entities 8
Chains and sequence ranges Author chain H； PDBConstruct 1–318； UniProt 1–318

NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial

Bos taurus

UniProt P42028

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain I； UniProt 37–212 Not recorded NADH-ubiquinone oxidoreductase chain 3 × 1 （Q8WAB4） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （P42026） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （P23709） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （P17694） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P04394） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P25708） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P15690） NADH-ubiquinone oxidoreductase chain 1 × 1 （Q6EMT1） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03924） NADH-ubiquinone oxidoreductase chain 4L × 1 （Q6EMS3） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03920） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03910） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03892） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （P34942） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （P34943） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q02375） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P23934） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q02370） Acyl carrier protein, mitochondrial × 1 （Q9CR21） Acyl carrier protein, mitochondrial × 1 （P52505） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （P23935） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q02366） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P42029） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q8HXG6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q95KV7） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （Q02377） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q02371） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q02376） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q02827） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q02379） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （Q02378） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q8HXG5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q02380） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q02367） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q02374） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q02365） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q02372） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （P48305） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q02369） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q02368） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q02373） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （O97725） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q05752） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） FME N-FORMYLMETHIONINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 2.80 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9W2V Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5LC5 Structure of mammalian respiratory Complex I, class2 Deposited 2016-06-19 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain I 37–212（176 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 4.35 Å
5LDW Structure of mammalian respiratory Complex I, class1 Deposited 2016-06-28 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain I 37–212（176 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus present in a cold room, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 4.27 Å
5LDX Structure of mammalian respiratory Complex I, class3. Deposited 2016-06-28 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain I 37–212（176 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer 20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7；pH 8
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 5.60 Å
5O31 Mitochondrial complex I in the deactive state Deposited 2017-05-23 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain I 37–212（176 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.13 Å
7DGQ Activity optimized supercomplex state1 Deposited 2020-11-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain E 37–212（176 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 CU COPPER (II) ION × 3 HEA HEME-A × 2 MG MAGNESIUM ION × 1 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 UQ2 UBIQUINONE-2 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.00 Å
7DGR Activity optimized supercomplex state2 Deposited 2020-11-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain E 37–212（176 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 3 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.60 Å
7DGS Activity optimized supercomplex state3 Deposited 2020-11-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain E 37–212（176 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 7.80 Å
7DGZ Activity optimized complex I (closed form) Deposited 2020-11-12 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain E 37–212（176 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
7DH0 Activity optimized complex I (open form) Deposited 2020-11-12 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain E 37–212（176 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
7DKF Activity optimized supercomplex state4 Deposited 2020-11-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain E2 37–212（176 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 8.30 Å
7QSD Bovine complex I in the active state at 3.1 A Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain I 1–212（212 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 4 LMT DODECYL-BETA-D-MALTOSIDE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7QSK Bovine complex I in lipid nanodisc, Active-Q10 Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain I 1–212（212 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.84 Å
7QSL Bovine complex I in lipid nanodisc, Active-apo Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain I 1–212（212 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.76 Å
7QSM Bovine complex I in lipid nanodisc, Deactive-ligand (composite) Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain I 1–212（212 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 GOL GLYCEROL × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.30 Å
7QSN Bovine complex I in lipid nanodisc, Deactive-apo Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain I 1–212（212 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.81 Å
7QSO Bovine complex I in lipid nanodisc, State 3 (Slack) Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain I 1–212（212 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 6 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 2 CDL CARDIOLIPIN × 6 U10 UBIQUINONE-10 × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.02 Å
7R41 Bovine complex I in the presence of IM1761092, active class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain I 1–212（212 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R42 Bovine complex I in the presence of IM1761092, active class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain I 1–212（212 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R43 Bovine complex I in the presence of IM1761092, active class iii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain I 1–212（212 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 CDL CARDIOLIPIN × 5 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R44 Bovine complex I in the presence of IM1761092, active class iv (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain I 1–212（212 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R45 Bovine complex I in the presence of IM1761092, deactive class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain I 1–212（212 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R46 Bovine complex I in the presence of IM1761092, deactive class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain I 1–212（212 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 11 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R47 Bovine complex I in the presence of IM1761092, deactive class iii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain I 1–212（212 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R48 Bovine complex I in the presence of IM1761092, deactive class iv (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain I 1–212（212 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4C Bovine complex I in the presence of IM1761092, deactive class v (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain I 1–212（212 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4D Bovine complex I in the presence of IM1761092, deactive class vi (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain I 1–212（212 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4F Bovine complex I in the presence of IM1761092, slack class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain I 1–212（212 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 7 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 CDL CARDIOLIPIN × 6 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R4G Bovine complex I in the presence of IM1761092, slack class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain I 1–212（212 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 10 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8Q0A Inward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain I 1–212（212 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 8 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0F Inward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain I 1–212（212 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 21 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0J Inward-facing, slack proteoliposome complex I at 3.8 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain I 1–212（212 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.80 Å
8Q0M Outward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain I 1–212（212 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0O Outward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain I 1–212（212 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0Q Outward-facing, slack proteoliposome complex I at 3.6 A. Initially purified in DDM Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain I 1–212（212 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.60 Å
8Q1P Inward-facing, open2 proteoliposome complex I at 2.9 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain I 1–212（212 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q1U Inward-facing, open1 proteoliposome complex I at 3.3 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain I 1–212（212 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.30 Å
8Q1Y Outward-facing, open2 proteoliposome complex I at 2.6 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain I 1–212（212 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q25 Outward-facing, open1 proteoliposome complex I at 2.8 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain I 1–212（212 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.80 Å
8Q45 Inward-facing, closed proteoliposome complex I at 2.7 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain I 1–212（212 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.70 Å
8Q46 Inward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain I 1–212（212 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q47 Inward-facing, open1 proteoliposome complex I at 2.9 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain I 1–212（212 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 26 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q48 Outward-facing, closed proteoliposome complex I at 2.5 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain I 1–212（212 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 35 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.50 Å
8Q49 Outward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain I 1–212（212 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 FVH [[(2~{R},3~{S},4~{R},5~{R})-5-[(3~{R})-3-aminocarbonylpiperidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2~{R},3~{R},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3-oxidanyl-4-phosphonooxy-oxolan-2-yl]methyl hydrogen phosphate × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q4A Outward-facing, open1 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain I 1–212（212 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
9SMF Reduced bovine complex I in lipid nanodisc, NADH-active-Q10 Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain I 1–212（212 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.51 Å
9SMG Reduced bovine complex I in lipid nanodisc, NADH-active-altQ10 Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain I 1–212（212 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.42 Å
9SMH Reduced bovine complex I in lipid nanodisc, NADH-active-DDM Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain I 1–212（212 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.61 Å
9SMI Reduced bovine complex I in lipid nanodisc, NADH-deactive Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain I 1–212（212 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 27 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 LMT DODECYL-BETA-D-MALTOSIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 CHD CHOLIC ACID × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.01 Å
9W2U Cryo-EM structure of complex I on the bovine heart submitochondrial particles, open Deposited 2025-07-28 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain I 37–212（176 aa）
Not recorded FME N-FORMYLMETHIONINE × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å

49 other PDB entries and 49 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUS8_BOVIN
Isoform —
PDB entities 9
Chains and sequence ranges Author chain I； PDBConstruct 1–176； UniProt 37–212

NADH-ubiquinone oxidoreductase chain 6

Bos taurus

UniProt P03924

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain J； UniProt 1–175 Non-standard monomer：Yes (specific site not provided by mmCIF) NADH-ubiquinone oxidoreductase chain 3 × 1 （Q8WAB4） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （P42026） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （P23709） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （P17694） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P04394） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P25708） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P15690） NADH-ubiquinone oxidoreductase chain 1 × 1 （Q6EMT1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （P42028） NADH-ubiquinone oxidoreductase chain 4L × 1 （Q6EMS3） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03920） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03910） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03892） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （P34942） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （P34943） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q02375） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P23934） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q02370） Acyl carrier protein, mitochondrial × 1 （Q9CR21） Acyl carrier protein, mitochondrial × 1 （P52505） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （P23935） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q02366） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P42029） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q8HXG6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q95KV7） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （Q02377） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q02371） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q02376） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q02827） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q02379） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （Q02378） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q8HXG5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q02380） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q02367） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q02374） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q02365） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q02372） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （P48305） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q02369） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q02368） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q02373） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （O97725） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q05752） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） FME N-FORMYLMETHIONINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 2.80 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9W2V Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5LC5 Structure of mammalian respiratory Complex I, class2 Deposited 2016-06-19 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain J 2–172（171 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 4.35 Å
5LDW Structure of mammalian respiratory Complex I, class1 Deposited 2016-06-28 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain J 1–175（175 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus present in a cold room, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 4.27 Å
5LDX Structure of mammalian respiratory Complex I, class3. Deposited 2016-06-28 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain J 1–175（175 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer 20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7；pH 8
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 5.60 Å
5O31 Mitochondrial complex I in the deactive state Deposited 2017-05-23 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain J 1–175（175 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.13 Å
7DGQ Activity optimized supercomplex state1 Deposited 2020-11-12 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain 7 1–175（175 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 CU COPPER (II) ION × 3 HEA HEME-A × 2 MG MAGNESIUM ION × 1 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 UQ2 UBIQUINONE-2 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.00 Å
7DGR Activity optimized supercomplex state2 Deposited 2020-11-12 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain 7 1–175（175 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 3 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.60 Å
7DGS Activity optimized supercomplex state3 Deposited 2020-11-12 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain 7 1–175（175 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 7.80 Å
7DGZ Activity optimized complex I (closed form) Deposited 2020-11-12 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain 7 1–175（175 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
7DH0 Activity optimized complex I (open form) Deposited 2020-11-12 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain 7 1–175（175 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
7DKF Activity optimized supercomplex state4 Deposited 2020-11-24 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain 72 1–175（175 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 8.30 Å
7QSD Bovine complex I in the active state at 3.1 A Deposited 2022-01-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain J 1–175（175 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 4 LMT DODECYL-BETA-D-MALTOSIDE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7QSK Bovine complex I in lipid nanodisc, Active-Q10 Deposited 2022-01-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain J 1–175（175 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.84 Å
7QSL Bovine complex I in lipid nanodisc, Active-apo Deposited 2022-01-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain J 1–175（175 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.76 Å
7QSM Bovine complex I in lipid nanodisc, Deactive-ligand (composite) Deposited 2022-01-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain J 1–175（175 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 GOL GLYCEROL × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.30 Å
7QSN Bovine complex I in lipid nanodisc, Deactive-apo Deposited 2022-01-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain J 1–175（175 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.81 Å
7QSO Bovine complex I in lipid nanodisc, State 3 (Slack) Deposited 2022-01-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain J 1–175（175 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 6 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 2 CDL CARDIOLIPIN × 6 U10 UBIQUINONE-10 × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.02 Å
7R41 Bovine complex I in the presence of IM1761092, active class i (Composite map) Deposited 2022-02-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain J 1–175（175 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R42 Bovine complex I in the presence of IM1761092, active class ii (Composite map) Deposited 2022-02-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain J 1–175（175 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R43 Bovine complex I in the presence of IM1761092, active class iii (Composite map) Deposited 2022-02-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain J 1–175（175 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 CDL CARDIOLIPIN × 5 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R44 Bovine complex I in the presence of IM1761092, active class iv (Composite map) Deposited 2022-02-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain J 1–175（175 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R45 Bovine complex I in the presence of IM1761092, deactive class i (Composite map) Deposited 2022-02-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain J 1–175（175 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R46 Bovine complex I in the presence of IM1761092, deactive class ii (Composite map) Deposited 2022-02-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain J 1–175（175 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 11 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R47 Bovine complex I in the presence of IM1761092, deactive class iii (Composite map) Deposited 2022-02-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain J 1–175（175 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R48 Bovine complex I in the presence of IM1761092, deactive class iv (Composite map) Deposited 2022-02-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain J 1–175（175 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4C Bovine complex I in the presence of IM1761092, deactive class v (Composite map) Deposited 2022-02-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain J 1–175（175 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4D Bovine complex I in the presence of IM1761092, deactive class vi (Composite map) Deposited 2022-02-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain J 1–175（175 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4F Bovine complex I in the presence of IM1761092, slack class i (Composite map) Deposited 2022-02-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain J 1–175（175 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 7 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 CDL CARDIOLIPIN × 6 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R4G Bovine complex I in the presence of IM1761092, slack class ii (Composite map) Deposited 2022-02-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain J 1–175（175 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 10 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8Q0A Inward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain J 1–175（175 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 8 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0F Inward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain J 1–175（175 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 21 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0J Inward-facing, slack proteoliposome complex I at 3.8 A. Initially purified in DDM. Deposited 2023-07-28 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain J 1–175（175 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.80 Å
8Q0M Outward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain J 1–175（175 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0O Outward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain J 1–175（175 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0Q Outward-facing, slack proteoliposome complex I at 3.6 A. Initially purified in DDM Deposited 2023-07-28 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain J 1–175（175 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.60 Å
8Q1P Inward-facing, open2 proteoliposome complex I at 2.9 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain J 1–175（175 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q1U Inward-facing, open1 proteoliposome complex I at 3.3 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain J 1–175（175 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.30 Å
8Q1Y Outward-facing, open2 proteoliposome complex I at 2.6 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain J 1–175（175 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q25 Outward-facing, open1 proteoliposome complex I at 2.8 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain J 1–175（175 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.80 Å
8Q45 Inward-facing, closed proteoliposome complex I at 2.7 A. Initially purified in LMNG. Deposited 2023-08-05 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain J 1–175（175 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.70 Å
8Q46 Inward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain J 1–175（175 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q47 Inward-facing, open1 proteoliposome complex I at 2.9 A. Initially purified in LMNG. Deposited 2023-08-05 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain J 1–175（175 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 26 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q48 Outward-facing, closed proteoliposome complex I at 2.5 A. Initially purified in LMNG. Deposited 2023-08-05 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain J 1–175（175 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 35 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.50 Å
8Q49 Outward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain J 1–175（175 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 FVH [[(2~{R},3~{S},4~{R},5~{R})-5-[(3~{R})-3-aminocarbonylpiperidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2~{R},3~{R},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3-oxidanyl-4-phosphonooxy-oxolan-2-yl]methyl hydrogen phosphate × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q4A Outward-facing, open1 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain J 1–175（175 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
9SMF Reduced bovine complex I in lipid nanodisc, NADH-active-Q10 Deposited 2025-09-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain J 1–175（175 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.51 Å
9SMG Reduced bovine complex I in lipid nanodisc, NADH-active-altQ10 Deposited 2025-09-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain J 1–175（175 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.42 Å
9SMH Reduced bovine complex I in lipid nanodisc, NADH-active-DDM Deposited 2025-09-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain J 1–175（175 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.61 Å
9SMI Reduced bovine complex I in lipid nanodisc, NADH-deactive Deposited 2025-09-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain J 1–175（175 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 27 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 LMT DODECYL-BETA-D-MALTOSIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 CHD CHOLIC ACID × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.01 Å
9W2U Cryo-EM structure of complex I on the bovine heart submitochondrial particles, open Deposited 2025-07-28 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain J 1–175（175 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) FME N-FORMYLMETHIONINE × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å

49 other PDB entries and 49 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NU6M_BOVIN
Isoform —
PDB entities 10
Chains and sequence ranges Author chain J； PDBConstruct 1–175； UniProt 1–175

NADH-ubiquinone oxidoreductase chain 4L

Bos taurus

UniProt Q6EMS3

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain K； UniProt 1–98 Non-standard monomer：Yes (specific site not provided by mmCIF) NADH-ubiquinone oxidoreductase chain 3 × 1 （Q8WAB4） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （P42026） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （P23709） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （P17694） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P04394） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P25708） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P15690） NADH-ubiquinone oxidoreductase chain 1 × 1 （Q6EMT1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （P42028） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03924） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03920） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03910） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03892） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （P34942） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （P34943） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q02375） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P23934） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q02370） Acyl carrier protein, mitochondrial × 1 （Q9CR21） Acyl carrier protein, mitochondrial × 1 （P52505） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （P23935） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q02366） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P42029） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q8HXG6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q95KV7） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （Q02377） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q02371） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q02376） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q02827） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q02379） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （Q02378） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q8HXG5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q02380） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q02367） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q02374） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q02365） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q02372） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （P48305） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q02369） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q02368） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q02373） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （O97725） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q05752） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） FME N-FORMYLMETHIONINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 2.80 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9W2V Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
9W2U Cryo-EM structure of complex I on the bovine heart submitochondrial particles, open Deposited 2025-07-28 Different construct Different mutation/modification Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain K 2–98（97 aa）
Not recorded FME N-FORMYLMETHIONINE × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NU4LM_BOSIN
Isoform —
PDB entities 11
Chains and sequence ranges Author chain K； PDBConstruct 1–98； UniProt 1–98

NADH-ubiquinone oxidoreductase chain 5

Bos taurus

UniProt P03920

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain L； UniProt 2–606 Not recorded NADH-ubiquinone oxidoreductase chain 3 × 1 （Q8WAB4） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （P42026） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （P23709） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （P17694） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P04394） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P25708） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P15690） NADH-ubiquinone oxidoreductase chain 1 × 1 （Q6EMT1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （P42028） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03924） NADH-ubiquinone oxidoreductase chain 4L × 1 （Q6EMS3） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03910） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03892） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （P34942） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （P34943） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q02375） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P23934） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q02370） Acyl carrier protein, mitochondrial × 1 （Q9CR21） Acyl carrier protein, mitochondrial × 1 （P52505） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （P23935） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q02366） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P42029） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q8HXG6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q95KV7） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （Q02377） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q02371） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q02376） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q02827） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q02379） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （Q02378） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q8HXG5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q02380） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q02367） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q02374） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q02365） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q02372） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （P48305） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q02369） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q02368） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q02373） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （O97725） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q05752） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） FME N-FORMYLMETHIONINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 2.80 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9W2V Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5LC5 Structure of mammalian respiratory Complex I, class2 Deposited 2016-06-19 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain L 2–605（604 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 4.35 Å
5LDW Structure of mammalian respiratory Complex I, class1 Deposited 2016-06-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain L 1–606（606 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus present in a cold room, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 4.27 Å
5LDX Structure of mammalian respiratory Complex I, class3. Deposited 2016-06-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain L 1–606（606 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer 20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7；pH 8
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 5.60 Å
5O31 Mitochondrial complex I in the deactive state Deposited 2017-05-23 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain L 1–606（606 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.13 Å
7DGQ Activity optimized supercomplex state1 Deposited 2020-11-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain 6 1–606（606 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 CU COPPER (II) ION × 3 HEA HEME-A × 2 MG MAGNESIUM ION × 1 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 UQ2 UBIQUINONE-2 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.00 Å
7DGR Activity optimized supercomplex state2 Deposited 2020-11-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain 6 1–606（606 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 3 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.60 Å
7DGS Activity optimized supercomplex state3 Deposited 2020-11-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain 6 1–606（606 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 7.80 Å
7DGZ Activity optimized complex I (closed form) Deposited 2020-11-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain 6 1–606（606 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
7DH0 Activity optimized complex I (open form) Deposited 2020-11-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain 6 1–606（606 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
7DKF Activity optimized supercomplex state4 Deposited 2020-11-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain 62 1–606（606 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 8.30 Å
7QSD Bovine complex I in the active state at 3.1 A Deposited 2022-01-13 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain L 1–606（606 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 4 LMT DODECYL-BETA-D-MALTOSIDE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7QSK Bovine complex I in lipid nanodisc, Active-Q10 Deposited 2022-01-13 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain L 1–606（606 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.84 Å
7QSL Bovine complex I in lipid nanodisc, Active-apo Deposited 2022-01-13 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain L 1–606（606 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.76 Å
7QSM Bovine complex I in lipid nanodisc, Deactive-ligand (composite) Deposited 2022-01-13 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain L 1–606（606 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 GOL GLYCEROL × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.30 Å
7QSN Bovine complex I in lipid nanodisc, Deactive-apo Deposited 2022-01-13 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain L 1–606（606 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.81 Å
7QSO Bovine complex I in lipid nanodisc, State 3 (Slack) Deposited 2022-01-13 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain L 1–606（606 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 6 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 2 CDL CARDIOLIPIN × 6 U10 UBIQUINONE-10 × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.02 Å
7R41 Bovine complex I in the presence of IM1761092, active class i (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain L 1–606（606 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R42 Bovine complex I in the presence of IM1761092, active class ii (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain L 1–606（606 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R43 Bovine complex I in the presence of IM1761092, active class iii (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain L 1–606（606 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 CDL CARDIOLIPIN × 5 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R44 Bovine complex I in the presence of IM1761092, active class iv (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain L 1–606（606 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R45 Bovine complex I in the presence of IM1761092, deactive class i (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain L 1–606（606 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R46 Bovine complex I in the presence of IM1761092, deactive class ii (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain L 1–606（606 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 11 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R47 Bovine complex I in the presence of IM1761092, deactive class iii (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain L 1–606（606 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R48 Bovine complex I in the presence of IM1761092, deactive class iv (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain L 1–606（606 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4C Bovine complex I in the presence of IM1761092, deactive class v (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain L 1–606（606 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4D Bovine complex I in the presence of IM1761092, deactive class vi (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain L 1–606（606 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4F Bovine complex I in the presence of IM1761092, slack class i (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain L 1–606（606 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 7 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 CDL CARDIOLIPIN × 6 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R4G Bovine complex I in the presence of IM1761092, slack class ii (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain L 1–606（606 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 10 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8Q0A Inward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain L 1–606（606 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 8 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0F Inward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain L 1–606（606 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 21 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0J Inward-facing, slack proteoliposome complex I at 3.8 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain L 1–606（606 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.80 Å
8Q0M Outward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain L 1–606（606 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0O Outward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain L 1–606（606 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0Q Outward-facing, slack proteoliposome complex I at 3.6 A. Initially purified in DDM Deposited 2023-07-28 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain L 1–606（606 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.60 Å
8Q1P Inward-facing, open2 proteoliposome complex I at 2.9 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain L 1–606（606 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q1U Inward-facing, open1 proteoliposome complex I at 3.3 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain L 1–606（606 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.30 Å
8Q1Y Outward-facing, open2 proteoliposome complex I at 2.6 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain L 1–606（606 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q25 Outward-facing, open1 proteoliposome complex I at 2.8 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain L 1–606（606 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.80 Å
8Q45 Inward-facing, closed proteoliposome complex I at 2.7 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain L 1–606（606 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.70 Å
8Q46 Inward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain L 1–606（606 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q47 Inward-facing, open1 proteoliposome complex I at 2.9 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain L 1–606（606 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 26 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q48 Outward-facing, closed proteoliposome complex I at 2.5 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain L 1–606（606 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 35 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.50 Å
8Q49 Outward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain L 1–606（606 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 FVH [[(2~{R},3~{S},4~{R},5~{R})-5-[(3~{R})-3-aminocarbonylpiperidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2~{R},3~{R},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3-oxidanyl-4-phosphonooxy-oxolan-2-yl]methyl hydrogen phosphate × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q4A Outward-facing, open1 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain L 1–606（606 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
9SMF Reduced bovine complex I in lipid nanodisc, NADH-active-Q10 Deposited 2025-09-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain L 1–606（606 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.51 Å
9SMG Reduced bovine complex I in lipid nanodisc, NADH-active-altQ10 Deposited 2025-09-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain L 1–606（606 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.42 Å
9SMH Reduced bovine complex I in lipid nanodisc, NADH-active-DDM Deposited 2025-09-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain L 1–606（606 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.61 Å
9SMI Reduced bovine complex I in lipid nanodisc, NADH-deactive Deposited 2025-09-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain L 1–606（606 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 27 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 LMT DODECYL-BETA-D-MALTOSIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 CHD CHOLIC ACID × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.01 Å
9W2U Cryo-EM structure of complex I on the bovine heart submitochondrial particles, open Deposited 2025-07-28 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain L 2–606（605 aa）
Not recorded FME N-FORMYLMETHIONINE × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å

49 other PDB entries and 49 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NU5M_BOVIN
Isoform —
PDB entities 12
Chains and sequence ranges Author chain L； PDBConstruct 1–605； UniProt 2–606

NADH-ubiquinone oxidoreductase chain 4

Bos taurus

UniProt P03910

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain M； UniProt 2–459 Not recorded NADH-ubiquinone oxidoreductase chain 3 × 1 （Q8WAB4） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （P42026） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （P23709） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （P17694） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P04394） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P25708） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P15690） NADH-ubiquinone oxidoreductase chain 1 × 1 （Q6EMT1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （P42028） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03924） NADH-ubiquinone oxidoreductase chain 4L × 1 （Q6EMS3） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03920） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03892） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （P34942） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （P34943） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q02375） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P23934） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q02370） Acyl carrier protein, mitochondrial × 1 （Q9CR21） Acyl carrier protein, mitochondrial × 1 （P52505） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （P23935） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q02366） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P42029） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q8HXG6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q95KV7） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （Q02377） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q02371） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q02376） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q02827） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q02379） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （Q02378） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q8HXG5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q02380） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q02367） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q02374） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q02365） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q02372） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （P48305） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q02369） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q02368） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q02373） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （O97725） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q05752） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） FME N-FORMYLMETHIONINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 2.80 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9W2V Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5LC5 Structure of mammalian respiratory Complex I, class2 Deposited 2016-06-19 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain M 3–459（457 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 4.35 Å
5LDW Structure of mammalian respiratory Complex I, class1 Deposited 2016-06-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain M 1–459（459 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus present in a cold room, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 4.27 Å
5LDX Structure of mammalian respiratory Complex I, class3. Deposited 2016-06-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain M 1–459（459 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer 20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7；pH 8
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 5.60 Å
5O31 Mitochondrial complex I in the deactive state Deposited 2017-05-23 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain M 1–459（459 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.13 Å
7DGQ Activity optimized supercomplex state1 Deposited 2020-11-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain 4 1–459（459 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 CU COPPER (II) ION × 3 HEA HEME-A × 2 MG MAGNESIUM ION × 1 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 UQ2 UBIQUINONE-2 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.00 Å
7DGR Activity optimized supercomplex state2 Deposited 2020-11-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain 4 1–459（459 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 3 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.60 Å
7DGS Activity optimized supercomplex state3 Deposited 2020-11-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain 4 1–459（459 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 7.80 Å
7DGZ Activity optimized complex I (closed form) Deposited 2020-11-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain 4 1–459（459 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
7DH0 Activity optimized complex I (open form) Deposited 2020-11-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain 4 1–459（459 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
7DKF Activity optimized supercomplex state4 Deposited 2020-11-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain 42 1–459（459 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 8.30 Å
7QSD Bovine complex I in the active state at 3.1 A Deposited 2022-01-13 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain M 1–459（459 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 4 LMT DODECYL-BETA-D-MALTOSIDE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7QSK Bovine complex I in lipid nanodisc, Active-Q10 Deposited 2022-01-13 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain M 1–459（459 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.84 Å
7QSL Bovine complex I in lipid nanodisc, Active-apo Deposited 2022-01-13 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain M 1–459（459 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.76 Å
7QSM Bovine complex I in lipid nanodisc, Deactive-ligand (composite) Deposited 2022-01-13 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain M 1–459（459 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 GOL GLYCEROL × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.30 Å
7QSN Bovine complex I in lipid nanodisc, Deactive-apo Deposited 2022-01-13 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain M 1–459（459 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.81 Å
7QSO Bovine complex I in lipid nanodisc, State 3 (Slack) Deposited 2022-01-13 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain M 1–459（459 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 6 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 2 CDL CARDIOLIPIN × 6 U10 UBIQUINONE-10 × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.02 Å
7R41 Bovine complex I in the presence of IM1761092, active class i (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain M 1–459（459 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R42 Bovine complex I in the presence of IM1761092, active class ii (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain M 1–459（459 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R43 Bovine complex I in the presence of IM1761092, active class iii (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain M 1–459（459 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 CDL CARDIOLIPIN × 5 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R44 Bovine complex I in the presence of IM1761092, active class iv (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain M 1–459（459 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R45 Bovine complex I in the presence of IM1761092, deactive class i (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain M 1–459（459 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R46 Bovine complex I in the presence of IM1761092, deactive class ii (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain M 1–459（459 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 11 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R47 Bovine complex I in the presence of IM1761092, deactive class iii (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain M 1–459（459 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R48 Bovine complex I in the presence of IM1761092, deactive class iv (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain M 1–459（459 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4C Bovine complex I in the presence of IM1761092, deactive class v (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain M 1–459（459 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4D Bovine complex I in the presence of IM1761092, deactive class vi (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain M 1–459（459 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4F Bovine complex I in the presence of IM1761092, slack class i (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain M 1–459（459 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 7 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 CDL CARDIOLIPIN × 6 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R4G Bovine complex I in the presence of IM1761092, slack class ii (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain M 1–459（459 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 10 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8Q0A Inward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain M 1–459（459 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 8 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0F Inward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain M 1–459（459 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 21 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0J Inward-facing, slack proteoliposome complex I at 3.8 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain M 1–459（459 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.80 Å
8Q0M Outward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain M 1–459（459 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0O Outward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain M 1–459（459 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0Q Outward-facing, slack proteoliposome complex I at 3.6 A. Initially purified in DDM Deposited 2023-07-28 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain M 1–459（459 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.60 Å
8Q1P Inward-facing, open2 proteoliposome complex I at 2.9 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain M 1–459（459 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q1U Inward-facing, open1 proteoliposome complex I at 3.3 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain M 1–459（459 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.30 Å
8Q1Y Outward-facing, open2 proteoliposome complex I at 2.6 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain M 1–459（459 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q25 Outward-facing, open1 proteoliposome complex I at 2.8 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain M 1–459（459 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.80 Å
8Q45 Inward-facing, closed proteoliposome complex I at 2.7 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain M 1–459（459 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.70 Å
8Q46 Inward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain M 1–459（459 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q47 Inward-facing, open1 proteoliposome complex I at 2.9 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain M 1–459（459 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 26 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q48 Outward-facing, closed proteoliposome complex I at 2.5 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain M 1–459（459 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 35 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.50 Å
8Q49 Outward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain M 1–459（459 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 FVH [[(2~{R},3~{S},4~{R},5~{R})-5-[(3~{R})-3-aminocarbonylpiperidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2~{R},3~{R},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3-oxidanyl-4-phosphonooxy-oxolan-2-yl]methyl hydrogen phosphate × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q4A Outward-facing, open1 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain M 1–459（459 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
9SMF Reduced bovine complex I in lipid nanodisc, NADH-active-Q10 Deposited 2025-09-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain M 1–459（459 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.51 Å
9SMG Reduced bovine complex I in lipid nanodisc, NADH-active-altQ10 Deposited 2025-09-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain M 1–459（459 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.42 Å
9SMH Reduced bovine complex I in lipid nanodisc, NADH-active-DDM Deposited 2025-09-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain M 1–459（459 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.61 Å
9SMI Reduced bovine complex I in lipid nanodisc, NADH-deactive Deposited 2025-09-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain M 1–459（459 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 27 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 LMT DODECYL-BETA-D-MALTOSIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 CHD CHOLIC ACID × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.01 Å
9W2U Cryo-EM structure of complex I on the bovine heart submitochondrial particles, open Deposited 2025-07-28 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain M 2–459（458 aa）
Not recorded FME N-FORMYLMETHIONINE × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å

49 other PDB entries and 49 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NU4M_BOVIN
Isoform —
PDB entities 13
Chains and sequence ranges Author chain M； PDBConstruct 1–458； UniProt 2–459

NADH-ubiquinone oxidoreductase chain 2

Bos taurus

UniProt P03892

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain N； UniProt 2–347 Not recorded NADH-ubiquinone oxidoreductase chain 3 × 1 （Q8WAB4） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （P42026） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （P23709） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （P17694） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P04394） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P25708） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P15690） NADH-ubiquinone oxidoreductase chain 1 × 1 （Q6EMT1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （P42028） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03924） NADH-ubiquinone oxidoreductase chain 4L × 1 （Q6EMS3） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03920） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03910） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （P34942） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （P34943） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q02375） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P23934） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q02370） Acyl carrier protein, mitochondrial × 1 （Q9CR21） Acyl carrier protein, mitochondrial × 1 （P52505） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （P23935） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q02366） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P42029） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q8HXG6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q95KV7） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （Q02377） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q02371） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q02376） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q02827） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q02379） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （Q02378） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q8HXG5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q02380） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q02367） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q02374） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q02365） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q02372） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （P48305） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q02369） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q02368） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q02373） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （O97725） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q05752） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） FME N-FORMYLMETHIONINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 2.80 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9W2V Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5LC5 Structure of mammalian respiratory Complex I, class2 Deposited 2016-06-19 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain N 2–345（344 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 4.35 Å
5LDW Structure of mammalian respiratory Complex I, class1 Deposited 2016-06-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain N 1–347（347 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus present in a cold room, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 4.27 Å
5LDX Structure of mammalian respiratory Complex I, class3. Deposited 2016-06-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain N 1–347（347 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer 20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7；pH 8
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 5.60 Å
5O31 Mitochondrial complex I in the deactive state Deposited 2017-05-23 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain N 1–347（347 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.13 Å
7DGQ Activity optimized supercomplex state1 Deposited 2020-11-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain 2 1–347（347 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 CU COPPER (II) ION × 3 HEA HEME-A × 2 MG MAGNESIUM ION × 1 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 UQ2 UBIQUINONE-2 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.00 Å
7DGR Activity optimized supercomplex state2 Deposited 2020-11-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain 2 1–347（347 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 3 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.60 Å
7DGS Activity optimized supercomplex state3 Deposited 2020-11-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain 2 1–347（347 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 7.80 Å
7DGZ Activity optimized complex I (closed form) Deposited 2020-11-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain 2 1–347（347 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
7DH0 Activity optimized complex I (open form) Deposited 2020-11-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain 2 1–347（347 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
7DKF Activity optimized supercomplex state4 Deposited 2020-11-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain 22 1–347（347 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 8.30 Å
7QSD Bovine complex I in the active state at 3.1 A Deposited 2022-01-13 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain N 1–347（347 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 4 LMT DODECYL-BETA-D-MALTOSIDE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7QSK Bovine complex I in lipid nanodisc, Active-Q10 Deposited 2022-01-13 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain N 1–347（347 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.84 Å
7QSL Bovine complex I in lipid nanodisc, Active-apo Deposited 2022-01-13 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain N 1–347（347 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.76 Å
7QSM Bovine complex I in lipid nanodisc, Deactive-ligand (composite) Deposited 2022-01-13 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain N 1–347（347 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 GOL GLYCEROL × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.30 Å
7QSN Bovine complex I in lipid nanodisc, Deactive-apo Deposited 2022-01-13 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain N 1–347（347 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.81 Å
7QSO Bovine complex I in lipid nanodisc, State 3 (Slack) Deposited 2022-01-13 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain N 1–347（347 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 6 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 2 CDL CARDIOLIPIN × 6 U10 UBIQUINONE-10 × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.02 Å
7R41 Bovine complex I in the presence of IM1761092, active class i (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain N 1–347（347 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R42 Bovine complex I in the presence of IM1761092, active class ii (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain N 1–347（347 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R43 Bovine complex I in the presence of IM1761092, active class iii (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain N 1–347（347 aa）
Mutation：N278K Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 CDL CARDIOLIPIN × 5 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R44 Bovine complex I in the presence of IM1761092, active class iv (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain N 1–347（347 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R45 Bovine complex I in the presence of IM1761092, deactive class i (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain N 1–347（347 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R46 Bovine complex I in the presence of IM1761092, deactive class ii (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain N 1–347（347 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 11 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R47 Bovine complex I in the presence of IM1761092, deactive class iii (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain N 1–347（347 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R48 Bovine complex I in the presence of IM1761092, deactive class iv (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain N 1–347（347 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4C Bovine complex I in the presence of IM1761092, deactive class v (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain N 1–347（347 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4D Bovine complex I in the presence of IM1761092, deactive class vi (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain N 1–347（347 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4F Bovine complex I in the presence of IM1761092, slack class i (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain N 1–347（347 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 7 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 CDL CARDIOLIPIN × 6 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R4G Bovine complex I in the presence of IM1761092, slack class ii (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain N 1–347（347 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 10 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8Q0A Inward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain N 1–347（347 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 8 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0F Inward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain N 1–347（347 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 21 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0J Inward-facing, slack proteoliposome complex I at 3.8 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain N 1–347（347 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.80 Å
8Q0M Outward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain N 1–347（347 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0O Outward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain N 1–347（347 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0Q Outward-facing, slack proteoliposome complex I at 3.6 A. Initially purified in DDM Deposited 2023-07-28 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain N 1–347（347 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.60 Å
8Q1P Inward-facing, open2 proteoliposome complex I at 2.9 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain N 1–347（347 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q1U Inward-facing, open1 proteoliposome complex I at 3.3 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain N 1–347（347 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.30 Å
8Q1Y Outward-facing, open2 proteoliposome complex I at 2.6 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain N 1–347（347 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q25 Outward-facing, open1 proteoliposome complex I at 2.8 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain N 1–347（347 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.80 Å
8Q45 Inward-facing, closed proteoliposome complex I at 2.7 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain N 1–347（347 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.70 Å
8Q46 Inward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain N 1–347（347 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q47 Inward-facing, open1 proteoliposome complex I at 2.9 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain N 1–347（347 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 26 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q48 Outward-facing, closed proteoliposome complex I at 2.5 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain N 1–347（347 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 35 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.50 Å
8Q49 Outward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain N 1–347（347 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 FVH [[(2~{R},3~{S},4~{R},5~{R})-5-[(3~{R})-3-aminocarbonylpiperidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2~{R},3~{R},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3-oxidanyl-4-phosphonooxy-oxolan-2-yl]methyl hydrogen phosphate × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q4A Outward-facing, open1 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain N 1–347（347 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
9SMF Reduced bovine complex I in lipid nanodisc, NADH-active-Q10 Deposited 2025-09-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain N 1–347（347 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.51 Å
9SMG Reduced bovine complex I in lipid nanodisc, NADH-active-altQ10 Deposited 2025-09-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain N 1–347（347 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.42 Å
9SMH Reduced bovine complex I in lipid nanodisc, NADH-active-DDM Deposited 2025-09-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain N 1–347（347 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.61 Å
9SMI Reduced bovine complex I in lipid nanodisc, NADH-deactive Deposited 2025-09-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain N 1–347（347 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 27 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 LMT DODECYL-BETA-D-MALTOSIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 CHD CHOLIC ACID × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.01 Å
9W2U Cryo-EM structure of complex I on the bovine heart submitochondrial particles, open Deposited 2025-07-28 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain N 2–347（346 aa）
Not recorded FME N-FORMYLMETHIONINE × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å

49 other PDB entries and 49 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NU2M_BOVIN
Isoform —
PDB entities 14
Chains and sequence ranges Author chain N； PDBConstruct 1–346； UniProt 2–347

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial

Bos taurus

UniProt P34942

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain O； UniProt 24–343 Not recorded NADH-ubiquinone oxidoreductase chain 3 × 1 （Q8WAB4） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （P42026） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （P23709） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （P17694） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P04394） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P25708） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P15690） NADH-ubiquinone oxidoreductase chain 1 × 1 （Q6EMT1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （P42028） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03924） NADH-ubiquinone oxidoreductase chain 4L × 1 （Q6EMS3） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03920） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03910） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03892） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （P34943） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q02375） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P23934） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q02370） Acyl carrier protein, mitochondrial × 1 （Q9CR21） Acyl carrier protein, mitochondrial × 1 （P52505） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （P23935） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q02366） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P42029） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q8HXG6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q95KV7） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （Q02377） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q02371） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q02376） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q02827） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q02379） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （Q02378） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q8HXG5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q02380） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q02367） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q02374） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q02365） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q02372） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （P48305） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q02369） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q02368） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q02373） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （O97725） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q05752） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） FME N-FORMYLMETHIONINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 2.80 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9W2V Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5LC5 Structure of mammalian respiratory Complex I, class2 Deposited 2016-06-19 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain O 114–341（228 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 4.35 Å
5LDW Structure of mammalian respiratory Complex I, class1 Deposited 2016-06-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain O 114–343（230 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus present in a cold room, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 4.27 Å
5LDX Structure of mammalian respiratory Complex I, class3. Deposited 2016-06-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain O 114–341（228 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer 20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7；pH 8
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 5.60 Å
5O31 Mitochondrial complex I in the deactive state Deposited 2017-05-23 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain O 24–343（320 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.13 Å
7DGQ Activity optimized supercomplex state1 Deposited 2020-11-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain S 24–343（320 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 CU COPPER (II) ION × 3 HEA HEME-A × 2 MG MAGNESIUM ION × 1 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 UQ2 UBIQUINONE-2 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.00 Å
7DGR Activity optimized supercomplex state2 Deposited 2020-11-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain S 24–343（320 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 3 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.60 Å
7DGS Activity optimized supercomplex state3 Deposited 2020-11-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain S 24–343（320 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 7.80 Å
7DGZ Activity optimized complex I (closed form) Deposited 2020-11-12 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain S 24–343（320 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
7DH0 Activity optimized complex I (open form) Deposited 2020-11-12 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain S 24–343（320 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
7DKF Activity optimized supercomplex state4 Deposited 2020-11-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain S2 24–343（320 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 8.30 Å
7QSD Bovine complex I in the active state at 3.1 A Deposited 2022-01-13 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain O 1–343（343 aa）
Mutation：N255K (N278K) PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 4 LMT DODECYL-BETA-D-MALTOSIDE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7QSK Bovine complex I in lipid nanodisc, Active-Q10 Deposited 2022-01-13 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain O 1–343（343 aa）
Mutation：N255K (N278K) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.84 Å
7QSL Bovine complex I in lipid nanodisc, Active-apo Deposited 2022-01-13 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain O 1–343（343 aa）
Mutation：N255K (N278K) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.76 Å
7QSM Bovine complex I in lipid nanodisc, Deactive-ligand (composite) Deposited 2022-01-13 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain O 1–343（343 aa）
Mutation：N255K (N278K) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 GOL GLYCEROL × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.30 Å
7QSN Bovine complex I in lipid nanodisc, Deactive-apo Deposited 2022-01-13 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain O 1–343（343 aa）
Mutation：N255K (N278K) PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.81 Å
7QSO Bovine complex I in lipid nanodisc, State 3 (Slack) Deposited 2022-01-13 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain O 1–343（343 aa）
Mutation：N255K (N278K) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 6 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 2 CDL CARDIOLIPIN × 6 U10 UBIQUINONE-10 × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.02 Å
7R41 Bovine complex I in the presence of IM1761092, active class i (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain O 1–343（343 aa）
Mutation：N278K LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R42 Bovine complex I in the presence of IM1761092, active class ii (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain O 1–343（343 aa）
Mutation：N278K LMT DODECYL-BETA-D-MALTOSIDE × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R43 Bovine complex I in the presence of IM1761092, active class iii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain O 1–343（343 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 CDL CARDIOLIPIN × 5 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R44 Bovine complex I in the presence of IM1761092, active class iv (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain O 1–343（343 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R45 Bovine complex I in the presence of IM1761092, deactive class i (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain O 1–343（343 aa）
Mutation：N278K LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R46 Bovine complex I in the presence of IM1761092, deactive class ii (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain O 1–343（343 aa）
Mutation：N278K LMT DODECYL-BETA-D-MALTOSIDE × 11 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R47 Bovine complex I in the presence of IM1761092, deactive class iii (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain O 1–343（343 aa）
Mutation：N278K LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R48 Bovine complex I in the presence of IM1761092, deactive class iv (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain O 1–343（343 aa）
Mutation：N278K LMT DODECYL-BETA-D-MALTOSIDE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4C Bovine complex I in the presence of IM1761092, deactive class v (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain O 1–343（343 aa）
Mutation：N278K LMT DODECYL-BETA-D-MALTOSIDE × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4D Bovine complex I in the presence of IM1761092, deactive class vi (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain O 1–343（343 aa）
Mutation：N278K LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4F Bovine complex I in the presence of IM1761092, slack class i (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain O 1–343（343 aa）
Mutation：N278K LMT DODECYL-BETA-D-MALTOSIDE × 7 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 CDL CARDIOLIPIN × 6 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R4G Bovine complex I in the presence of IM1761092, slack class ii (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain O 1–343（343 aa）
Mutation：N278K 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 10 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8Q0A Inward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain O 1–343（343 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 8 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0F Inward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain O 1–343（343 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 21 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0J Inward-facing, slack proteoliposome complex I at 3.8 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain O 1–343（343 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.80 Å
8Q0M Outward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain O 1–343（343 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0O Outward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain O 1–343（343 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0Q Outward-facing, slack proteoliposome complex I at 3.6 A. Initially purified in DDM Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain O 1–343（343 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.60 Å
8Q1P Inward-facing, open2 proteoliposome complex I at 2.9 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain O 1–343（343 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q1U Inward-facing, open1 proteoliposome complex I at 3.3 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain O 1–343（343 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.30 Å
8Q1Y Outward-facing, open2 proteoliposome complex I at 2.6 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain O 1–343（343 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q25 Outward-facing, open1 proteoliposome complex I at 2.8 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain O 1–343（343 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.80 Å
8Q45 Inward-facing, closed proteoliposome complex I at 2.7 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain O 1–343（343 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.70 Å
8Q46 Inward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain O 1–343（343 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q47 Inward-facing, open1 proteoliposome complex I at 2.9 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain O 1–343（343 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 26 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q48 Outward-facing, closed proteoliposome complex I at 2.5 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain O 1–343（343 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 35 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.50 Å
8Q49 Outward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain O 1–343（343 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 FVH [[(2~{R},3~{S},4~{R},5~{R})-5-[(3~{R})-3-aminocarbonylpiperidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2~{R},3~{R},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3-oxidanyl-4-phosphonooxy-oxolan-2-yl]methyl hydrogen phosphate × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q4A Outward-facing, open1 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain O 1–343（343 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
9SMF Reduced bovine complex I in lipid nanodisc, NADH-active-Q10 Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain O 1–343（343 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.51 Å
9SMG Reduced bovine complex I in lipid nanodisc, NADH-active-altQ10 Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain O 1–343（343 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.42 Å
9SMH Reduced bovine complex I in lipid nanodisc, NADH-active-DDM Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain O 1–343（343 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.61 Å
9SMI Reduced bovine complex I in lipid nanodisc, NADH-deactive Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain O 1–343（343 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 27 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 LMT DODECYL-BETA-D-MALTOSIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 CHD CHOLIC ACID × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.01 Å
9W2U Cryo-EM structure of complex I on the bovine heart submitochondrial particles, open Deposited 2025-07-28 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain O 24–343（320 aa）
Not recorded FME N-FORMYLMETHIONINE × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å

49 other PDB entries and 49 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUAA_BOVIN
Isoform —
PDB entities 15
Chains and sequence ranges Author chain O； PDBConstruct 1–320； UniProt 24–343

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial

Bos taurus

UniProt P34943

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain P； UniProt 36–377 Not recorded NADH-ubiquinone oxidoreductase chain 3 × 1 （Q8WAB4） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （P42026） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （P23709） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （P17694） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P04394） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P25708） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P15690） NADH-ubiquinone oxidoreductase chain 1 × 1 （Q6EMT1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （P42028） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03924） NADH-ubiquinone oxidoreductase chain 4L × 1 （Q6EMS3） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03920） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03910） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03892） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （P34942） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q02375） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P23934） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q02370） Acyl carrier protein, mitochondrial × 1 （Q9CR21） Acyl carrier protein, mitochondrial × 1 （P52505） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （P23935） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q02366） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P42029） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q8HXG6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q95KV7） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （Q02377） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q02371） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q02376） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q02827） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q02379） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （Q02378） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q8HXG5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q02380） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q02367） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q02374） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q02365） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q02372） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （P48305） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q02369） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q02368） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q02373） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （O97725） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q05752） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） FME N-FORMYLMETHIONINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 2.80 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9W2V Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
7DGQ Activity optimized supercomplex state1 Deposited 2020-11-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain R 36–380（345 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 CU COPPER (II) ION × 3 HEA HEME-A × 2 MG MAGNESIUM ION × 1 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 UQ2 UBIQUINONE-2 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.00 Å
7DGR Activity optimized supercomplex state2 Deposited 2020-11-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain R 36–380（345 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 3 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.60 Å
7DGS Activity optimized supercomplex state3 Deposited 2020-11-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain R 36–380（345 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 7.80 Å
7DGZ Activity optimized complex I (closed form) Deposited 2020-11-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain R 36–380（345 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
7DH0 Activity optimized complex I (open form) Deposited 2020-11-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain R 36–380（345 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
7DKF Activity optimized supercomplex state4 Deposited 2020-11-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain R2 36–380（345 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 8.30 Å
7QSD Bovine complex I in the active state at 3.1 A Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain P 1–380（380 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 4 LMT DODECYL-BETA-D-MALTOSIDE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7QSK Bovine complex I in lipid nanodisc, Active-Q10 Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain P 1–380（380 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.84 Å
7QSL Bovine complex I in lipid nanodisc, Active-apo Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain P 1–380（380 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.76 Å
7QSM Bovine complex I in lipid nanodisc, Deactive-ligand (composite) Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain P 1–380（380 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 GOL GLYCEROL × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.30 Å
7QSN Bovine complex I in lipid nanodisc, Deactive-apo Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain P 1–380（380 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.81 Å
7QSO Bovine complex I in lipid nanodisc, State 3 (Slack) Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain P 1–380（380 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 6 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 2 CDL CARDIOLIPIN × 6 U10 UBIQUINONE-10 × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.02 Å
7R41 Bovine complex I in the presence of IM1761092, active class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain P 1–380（380 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R42 Bovine complex I in the presence of IM1761092, active class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain P 1–380（380 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R43 Bovine complex I in the presence of IM1761092, active class iii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain P 1–380（380 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 CDL CARDIOLIPIN × 5 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R44 Bovine complex I in the presence of IM1761092, active class iv (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain P 1–380（380 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R45 Bovine complex I in the presence of IM1761092, deactive class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain P 1–380（380 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R46 Bovine complex I in the presence of IM1761092, deactive class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain P 1–380（380 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 11 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R47 Bovine complex I in the presence of IM1761092, deactive class iii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain P 1–380（380 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R48 Bovine complex I in the presence of IM1761092, deactive class iv (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain P 1–380（380 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4C Bovine complex I in the presence of IM1761092, deactive class v (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain P 1–380（380 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4D Bovine complex I in the presence of IM1761092, deactive class vi (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain P 1–380（380 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4F Bovine complex I in the presence of IM1761092, slack class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain P 1–380（380 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 7 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 CDL CARDIOLIPIN × 6 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R4G Bovine complex I in the presence of IM1761092, slack class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain P 1–380（380 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 10 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8Q0A Inward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain P 1–380（380 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 8 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0F Inward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain P 1–380（380 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 21 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0J Inward-facing, slack proteoliposome complex I at 3.8 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain P 1–380（380 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.80 Å
8Q0M Outward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain P 1–380（380 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0O Outward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain P 1–380（380 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0Q Outward-facing, slack proteoliposome complex I at 3.6 A. Initially purified in DDM Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain P 1–380（380 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.60 Å
8Q1P Inward-facing, open2 proteoliposome complex I at 2.9 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain P 1–380（380 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q1U Inward-facing, open1 proteoliposome complex I at 3.3 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain P 1–380（380 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.30 Å
8Q1Y Outward-facing, open2 proteoliposome complex I at 2.6 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain P 1–380（380 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q25 Outward-facing, open1 proteoliposome complex I at 2.8 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain P 1–380（380 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.80 Å
8Q45 Inward-facing, closed proteoliposome complex I at 2.7 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain P 1–380（380 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.70 Å
8Q46 Inward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain P 1–380（380 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q47 Inward-facing, open1 proteoliposome complex I at 2.9 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain P 1–380（380 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 26 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q48 Outward-facing, closed proteoliposome complex I at 2.5 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain P 1–380（380 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 35 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.50 Å
8Q49 Outward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain P 1–380（380 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 FVH [[(2~{R},3~{S},4~{R},5~{R})-5-[(3~{R})-3-aminocarbonylpiperidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2~{R},3~{R},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3-oxidanyl-4-phosphonooxy-oxolan-2-yl]methyl hydrogen phosphate × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q4A Outward-facing, open1 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain P 1–380（380 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
9SMF Reduced bovine complex I in lipid nanodisc, NADH-active-Q10 Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain P 1–380（380 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.51 Å
9SMG Reduced bovine complex I in lipid nanodisc, NADH-active-altQ10 Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain P 1–380（380 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.42 Å
9SMH Reduced bovine complex I in lipid nanodisc, NADH-active-DDM Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain P 1–380（380 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.61 Å
9SMI Reduced bovine complex I in lipid nanodisc, NADH-deactive Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain P 1–380（380 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 27 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 LMT DODECYL-BETA-D-MALTOSIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 CHD CHOLIC ACID × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.01 Å
9W2U Cryo-EM structure of complex I on the bovine heart submitochondrial particles, open Deposited 2025-07-28 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain P 36–377（342 aa）
Not recorded FME N-FORMYLMETHIONINE × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å

45 other PDB entries and 45 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUA9_BOVIN
Isoform —
PDB entities 16
Chains and sequence ranges Author chain P； PDBConstruct 1–342； UniProt 36–377

NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial

Bos taurus

UniProt Q02375

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain Q； UniProt 47–175 Not recorded NADH-ubiquinone oxidoreductase chain 3 × 1 （Q8WAB4） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （P42026） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （P23709） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （P17694） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P04394） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P25708） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P15690） NADH-ubiquinone oxidoreductase chain 1 × 1 （Q6EMT1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （P42028） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03924） NADH-ubiquinone oxidoreductase chain 4L × 1 （Q6EMS3） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03920） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03910） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03892） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （P34942） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （P34943） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P23934） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q02370） Acyl carrier protein, mitochondrial × 1 （Q9CR21） Acyl carrier protein, mitochondrial × 1 （P52505） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （P23935） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q02366） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P42029） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q8HXG6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q95KV7） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （Q02377） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q02371） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q02376） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q02827） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q02379） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （Q02378） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q8HXG5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q02380） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q02367） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q02374） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q02365） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q02372） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （P48305） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q02369） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q02368） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q02373） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （O97725） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q05752） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） FME N-FORMYLMETHIONINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 2.80 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9W2V Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5O31 Mitochondrial complex I in the deactive state Deposited 2017-05-23 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Q 43–175（133 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.13 Å
7DGQ Activity optimized supercomplex state1 Deposited 2020-11-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain G 43–175（133 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 CU COPPER (II) ION × 3 HEA HEME-A × 2 MG MAGNESIUM ION × 1 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 UQ2 UBIQUINONE-2 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.00 Å
7DGR Activity optimized supercomplex state2 Deposited 2020-11-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain G 43–175（133 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 3 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.60 Å
7DGS Activity optimized supercomplex state3 Deposited 2020-11-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain G 43–175（133 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 7.80 Å
7DGZ Activity optimized complex I (closed form) Deposited 2020-11-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain G 43–175（133 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
7DH0 Activity optimized complex I (open form) Deposited 2020-11-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain G 43–175（133 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
7DKF Activity optimized supercomplex state4 Deposited 2020-11-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain G2 43–175（133 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 8.30 Å
7QSD Bovine complex I in the active state at 3.1 A Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Q 1–175（175 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 4 LMT DODECYL-BETA-D-MALTOSIDE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7QSK Bovine complex I in lipid nanodisc, Active-Q10 Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Q 1–175（175 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.84 Å
7QSL Bovine complex I in lipid nanodisc, Active-apo Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Q 1–175（175 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.76 Å
7QSM Bovine complex I in lipid nanodisc, Deactive-ligand (composite) Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Q 1–175（175 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 GOL GLYCEROL × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.30 Å
7QSN Bovine complex I in lipid nanodisc, Deactive-apo Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Q 1–175（175 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.81 Å
7QSO Bovine complex I in lipid nanodisc, State 3 (Slack) Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Q 1–175（175 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 6 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 2 CDL CARDIOLIPIN × 6 U10 UBIQUINONE-10 × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.02 Å
7R41 Bovine complex I in the presence of IM1761092, active class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Q 1–175（175 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R42 Bovine complex I in the presence of IM1761092, active class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Q 1–175（175 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R43 Bovine complex I in the presence of IM1761092, active class iii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Q 1–175（175 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 CDL CARDIOLIPIN × 5 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R44 Bovine complex I in the presence of IM1761092, active class iv (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Q 1–175（175 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R45 Bovine complex I in the presence of IM1761092, deactive class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Q 1–175（175 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R46 Bovine complex I in the presence of IM1761092, deactive class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Q 1–175（175 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 11 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R47 Bovine complex I in the presence of IM1761092, deactive class iii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Q 1–175（175 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R48 Bovine complex I in the presence of IM1761092, deactive class iv (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Q 1–175（175 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4C Bovine complex I in the presence of IM1761092, deactive class v (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Q 1–175（175 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4D Bovine complex I in the presence of IM1761092, deactive class vi (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Q 1–175（175 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4F Bovine complex I in the presence of IM1761092, slack class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Q 1–175（175 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 7 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 CDL CARDIOLIPIN × 6 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R4G Bovine complex I in the presence of IM1761092, slack class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Q 1–175（175 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 10 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8Q0A Inward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Q 1–175（175 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 8 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0F Inward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Q 1–175（175 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 21 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0J Inward-facing, slack proteoliposome complex I at 3.8 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Q 1–175（175 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.80 Å
8Q0M Outward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Q 1–175（175 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0O Outward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Q 1–175（175 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0Q Outward-facing, slack proteoliposome complex I at 3.6 A. Initially purified in DDM Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Q 1–175（175 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.60 Å
8Q1P Inward-facing, open2 proteoliposome complex I at 2.9 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Q 1–175（175 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q1U Inward-facing, open1 proteoliposome complex I at 3.3 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Q 1–175（175 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.30 Å
8Q1Y Outward-facing, open2 proteoliposome complex I at 2.6 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Q 1–175（175 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q25 Outward-facing, open1 proteoliposome complex I at 2.8 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Q 1–175（175 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.80 Å
8Q45 Inward-facing, closed proteoliposome complex I at 2.7 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Q 1–175（175 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.70 Å
8Q46 Inward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Q 1–175（175 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q47 Inward-facing, open1 proteoliposome complex I at 2.9 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Q 1–175（175 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 26 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q48 Outward-facing, closed proteoliposome complex I at 2.5 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Q 1–175（175 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 35 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.50 Å
8Q49 Outward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Q 1–175（175 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 FVH [[(2~{R},3~{S},4~{R},5~{R})-5-[(3~{R})-3-aminocarbonylpiperidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2~{R},3~{R},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3-oxidanyl-4-phosphonooxy-oxolan-2-yl]methyl hydrogen phosphate × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q4A Outward-facing, open1 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Q 1–175（175 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
9SMF Reduced bovine complex I in lipid nanodisc, NADH-active-Q10 Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Q 1–175（175 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.51 Å
9SMG Reduced bovine complex I in lipid nanodisc, NADH-active-altQ10 Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Q 1–175（175 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.42 Å
9SMH Reduced bovine complex I in lipid nanodisc, NADH-active-DDM Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Q 1–175（175 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.61 Å
9SMI Reduced bovine complex I in lipid nanodisc, NADH-deactive Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Q 1–175（175 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 27 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 LMT DODECYL-BETA-D-MALTOSIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 CHD CHOLIC ACID × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.01 Å
9W2U Cryo-EM structure of complex I on the bovine heart submitochondrial particles, open Deposited 2025-07-28 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Q 47–175（129 aa）
Not recorded FME N-FORMYLMETHIONINE × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å

46 other PDB entries and 46 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUS4_BOVIN
Isoform —
PDB entities 17
Chains and sequence ranges Author chain Q； PDBConstruct 1–129； UniProt 47–175

NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial

Bos taurus

UniProt P23934

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain R； UniProt 29–124 Not recorded NADH-ubiquinone oxidoreductase chain 3 × 1 （Q8WAB4） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （P42026） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （P23709） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （P17694） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P04394） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P25708） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P15690） NADH-ubiquinone oxidoreductase chain 1 × 1 （Q6EMT1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （P42028） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03924） NADH-ubiquinone oxidoreductase chain 4L × 1 （Q6EMS3） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03920） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03910） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03892） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （P34942） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （P34943） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q02375） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q02370） Acyl carrier protein, mitochondrial × 1 （Q9CR21） Acyl carrier protein, mitochondrial × 1 （P52505） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （P23935） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q02366） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P42029） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q8HXG6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q95KV7） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （Q02377） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q02371） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q02376） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q02827） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q02379） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （Q02378） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q8HXG5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q02380） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q02367） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q02374） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q02365） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q02372） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （P48305） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q02369） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q02368） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q02373） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （O97725） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q05752） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） FME N-FORMYLMETHIONINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 2.80 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9W2V Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5LC5 Structure of mammalian respiratory Complex I, class2 Deposited 2016-06-19 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain R 87–121（35 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 4.35 Å
5LDW Structure of mammalian respiratory Complex I, class1 Deposited 2016-06-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain R 87–121（35 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus present in a cold room, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 4.27 Å
5LDX Structure of mammalian respiratory Complex I, class3. Deposited 2016-06-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain R 87–121（35 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer 20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7；pH 8
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 5.60 Å
5O31 Mitochondrial complex I in the deactive state Deposited 2017-05-23 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain R 29–124（96 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.13 Å
7DGQ Activity optimized supercomplex state1 Deposited 2020-11-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain I 29–124（96 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 CU COPPER (II) ION × 3 HEA HEME-A × 2 MG MAGNESIUM ION × 1 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 UQ2 UBIQUINONE-2 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.00 Å
7DGR Activity optimized supercomplex state2 Deposited 2020-11-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain I 29–124（96 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 3 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.60 Å
7DGS Activity optimized supercomplex state3 Deposited 2020-11-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain I 29–124（96 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 7.80 Å
7DGZ Activity optimized complex I (closed form) Deposited 2020-11-12 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain I 29–124（96 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
7DH0 Activity optimized complex I (open form) Deposited 2020-11-12 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain I 29–124（96 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
7DKF Activity optimized supercomplex state4 Deposited 2020-11-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain I2 29–124（96 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 8.30 Å
7QSD Bovine complex I in the active state at 3.1 A Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain R 1–124（124 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 4 LMT DODECYL-BETA-D-MALTOSIDE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7QSK Bovine complex I in lipid nanodisc, Active-Q10 Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain R 1–124（124 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.84 Å
7QSL Bovine complex I in lipid nanodisc, Active-apo Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain R 1–124（124 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.76 Å
7QSM Bovine complex I in lipid nanodisc, Deactive-ligand (composite) Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain R 1–124（124 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 GOL GLYCEROL × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.30 Å
7QSN Bovine complex I in lipid nanodisc, Deactive-apo Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain R 1–124（124 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.81 Å
7QSO Bovine complex I in lipid nanodisc, State 3 (Slack) Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain R 1–124（124 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 6 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 2 CDL CARDIOLIPIN × 6 U10 UBIQUINONE-10 × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.02 Å
7R41 Bovine complex I in the presence of IM1761092, active class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain R 1–124（124 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R42 Bovine complex I in the presence of IM1761092, active class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain R 1–124（124 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R43 Bovine complex I in the presence of IM1761092, active class iii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain R 1–124（124 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 CDL CARDIOLIPIN × 5 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R44 Bovine complex I in the presence of IM1761092, active class iv (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain R 1–124（124 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R45 Bovine complex I in the presence of IM1761092, deactive class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain R 1–124（124 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R46 Bovine complex I in the presence of IM1761092, deactive class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain R 1–124（124 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 11 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R47 Bovine complex I in the presence of IM1761092, deactive class iii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain R 1–124（124 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R48 Bovine complex I in the presence of IM1761092, deactive class iv (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain R 1–124（124 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4C Bovine complex I in the presence of IM1761092, deactive class v (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain R 1–124（124 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4D Bovine complex I in the presence of IM1761092, deactive class vi (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain R 1–124（124 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4F Bovine complex I in the presence of IM1761092, slack class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain R 1–124（124 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 7 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 CDL CARDIOLIPIN × 6 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R4G Bovine complex I in the presence of IM1761092, slack class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain R 1–124（124 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 10 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8Q0A Inward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain R 1–124（124 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 8 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0F Inward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain R 1–124（124 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 21 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0J Inward-facing, slack proteoliposome complex I at 3.8 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain R 1–124（124 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.80 Å
8Q0M Outward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain R 1–124（124 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0O Outward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain R 1–124（124 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0Q Outward-facing, slack proteoliposome complex I at 3.6 A. Initially purified in DDM Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain R 1–124（124 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.60 Å
8Q1P Inward-facing, open2 proteoliposome complex I at 2.9 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain R 1–124（124 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q1U Inward-facing, open1 proteoliposome complex I at 3.3 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain R 1–124（124 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.30 Å
8Q1Y Outward-facing, open2 proteoliposome complex I at 2.6 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain R 1–124（124 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q25 Outward-facing, open1 proteoliposome complex I at 2.8 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain R 1–124（124 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.80 Å
8Q45 Inward-facing, closed proteoliposome complex I at 2.7 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain R 1–124（124 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.70 Å
8Q46 Inward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain R 1–124（124 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q47 Inward-facing, open1 proteoliposome complex I at 2.9 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain R 1–124（124 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 26 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q48 Outward-facing, closed proteoliposome complex I at 2.5 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain R 1–124（124 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 35 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.50 Å
8Q49 Outward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain R 1–124（124 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 FVH [[(2~{R},3~{S},4~{R},5~{R})-5-[(3~{R})-3-aminocarbonylpiperidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2~{R},3~{R},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3-oxidanyl-4-phosphonooxy-oxolan-2-yl]methyl hydrogen phosphate × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q4A Outward-facing, open1 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain R 1–124（124 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
9SMF Reduced bovine complex I in lipid nanodisc, NADH-active-Q10 Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain R 1–124（124 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.51 Å
9SMG Reduced bovine complex I in lipid nanodisc, NADH-active-altQ10 Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain R 1–124（124 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.42 Å
9SMH Reduced bovine complex I in lipid nanodisc, NADH-active-DDM Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain R 1–124（124 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.61 Å
9SMI Reduced bovine complex I in lipid nanodisc, NADH-deactive Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain R 1–124（124 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 27 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 LMT DODECYL-BETA-D-MALTOSIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 CHD CHOLIC ACID × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.01 Å
9W2U Cryo-EM structure of complex I on the bovine heart submitochondrial particles, open Deposited 2025-07-28 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain R 29–124（96 aa）
Not recorded FME N-FORMYLMETHIONINE × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å

49 other PDB entries and 49 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUS6_BOVIN
Isoform —
PDB entities 18
Chains and sequence ranges Author chain R； PDBConstruct 1–96； UniProt 29–124

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2

Bos taurus

UniProt Q02370

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain S； UniProt 13–99 Not recorded NADH-ubiquinone oxidoreductase chain 3 × 1 （Q8WAB4） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （P42026） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （P23709） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （P17694） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P04394） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P25708） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P15690） NADH-ubiquinone oxidoreductase chain 1 × 1 （Q6EMT1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （P42028） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03924） NADH-ubiquinone oxidoreductase chain 4L × 1 （Q6EMS3） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03920） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03910） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03892） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （P34942） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （P34943） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q02375） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P23934） Acyl carrier protein, mitochondrial × 1 （Q9CR21） Acyl carrier protein, mitochondrial × 1 （P52505） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （P23935） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q02366） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P42029） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q8HXG6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q95KV7） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （Q02377） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q02371） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q02376） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q02827） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q02379） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （Q02378） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q8HXG5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q02380） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q02367） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q02374） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q02365） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q02372） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （P48305） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q02369） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q02368） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q02373） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （O97725） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q05752） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） FME N-FORMYLMETHIONINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 2.80 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9W2V Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5LC5 Structure of mammalian respiratory Complex I, class2 Deposited 2016-06-19 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain S 17–96（80 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 4.35 Å
5LDW Structure of mammalian respiratory Complex I, class1 Deposited 2016-06-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain S 1–99（99 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus present in a cold room, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 4.27 Å
5LDX Structure of mammalian respiratory Complex I, class3. Deposited 2016-06-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain S 1–99（99 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer 20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7；pH 8
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 5.60 Å
5O31 Mitochondrial complex I in the deactive state Deposited 2017-05-23 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain S 2–99（98 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.13 Å
7DGQ Activity optimized supercomplex state1 Deposited 2020-11-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain K 2–99（98 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 CU COPPER (II) ION × 3 HEA HEME-A × 2 MG MAGNESIUM ION × 1 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 UQ2 UBIQUINONE-2 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.00 Å
7DGR Activity optimized supercomplex state2 Deposited 2020-11-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain K 2–99（98 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 3 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.60 Å
7DGS Activity optimized supercomplex state3 Deposited 2020-11-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain K 2–99（98 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 7.80 Å
7DGZ Activity optimized complex I (closed form) Deposited 2020-11-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain K 2–99（98 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
7DH0 Activity optimized complex I (open form) Deposited 2020-11-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain K 2–99（98 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
7DKF Activity optimized supercomplex state4 Deposited 2020-11-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain K2 2–99（98 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 8.30 Å
7QSD Bovine complex I in the active state at 3.1 A Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain S 1–99（99 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 4 LMT DODECYL-BETA-D-MALTOSIDE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7QSK Bovine complex I in lipid nanodisc, Active-Q10 Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain S 1–99（99 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.84 Å
7QSL Bovine complex I in lipid nanodisc, Active-apo Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain S 1–99（99 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.76 Å
7QSM Bovine complex I in lipid nanodisc, Deactive-ligand (composite) Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain S 1–99（99 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 GOL GLYCEROL × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.30 Å
7QSN Bovine complex I in lipid nanodisc, Deactive-apo Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain S 1–99（99 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.81 Å
7QSO Bovine complex I in lipid nanodisc, State 3 (Slack) Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain S 1–99（99 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 6 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 2 CDL CARDIOLIPIN × 6 U10 UBIQUINONE-10 × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.02 Å
7R41 Bovine complex I in the presence of IM1761092, active class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain S 1–99（99 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R42 Bovine complex I in the presence of IM1761092, active class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain S 1–99（99 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R43 Bovine complex I in the presence of IM1761092, active class iii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain S 1–99（99 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 CDL CARDIOLIPIN × 5 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R44 Bovine complex I in the presence of IM1761092, active class iv (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain S 1–99（99 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R45 Bovine complex I in the presence of IM1761092, deactive class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain S 1–99（99 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R46 Bovine complex I in the presence of IM1761092, deactive class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain S 1–99（99 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 11 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R47 Bovine complex I in the presence of IM1761092, deactive class iii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain S 1–99（99 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R48 Bovine complex I in the presence of IM1761092, deactive class iv (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain S 1–99（99 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4C Bovine complex I in the presence of IM1761092, deactive class v (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain S 1–99（99 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4D Bovine complex I in the presence of IM1761092, deactive class vi (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain S 1–99（99 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4F Bovine complex I in the presence of IM1761092, slack class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain S 1–99（99 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 7 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 CDL CARDIOLIPIN × 6 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R4G Bovine complex I in the presence of IM1761092, slack class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain S 1–99（99 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 10 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8Q0A Inward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain S 1–99（99 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 8 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0F Inward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain S 1–99（99 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 21 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0J Inward-facing, slack proteoliposome complex I at 3.8 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain S 1–99（99 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.80 Å
8Q0M Outward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain S 1–99（99 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0O Outward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain S 1–99（99 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0Q Outward-facing, slack proteoliposome complex I at 3.6 A. Initially purified in DDM Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain S 1–99（99 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.60 Å
8Q1P Inward-facing, open2 proteoliposome complex I at 2.9 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain S 1–99（99 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q1U Inward-facing, open1 proteoliposome complex I at 3.3 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain S 1–99（99 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.30 Å
8Q1Y Outward-facing, open2 proteoliposome complex I at 2.6 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain S 1–99（99 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q25 Outward-facing, open1 proteoliposome complex I at 2.8 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain S 1–99（99 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.80 Å
8Q45 Inward-facing, closed proteoliposome complex I at 2.7 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain S 1–99（99 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.70 Å
8Q46 Inward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain S 1–99（99 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q47 Inward-facing, open1 proteoliposome complex I at 2.9 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain S 1–99（99 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 26 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q48 Outward-facing, closed proteoliposome complex I at 2.5 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain S 1–99（99 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 35 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.50 Å
8Q49 Outward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain S 1–99（99 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 FVH [[(2~{R},3~{S},4~{R},5~{R})-5-[(3~{R})-3-aminocarbonylpiperidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2~{R},3~{R},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3-oxidanyl-4-phosphonooxy-oxolan-2-yl]methyl hydrogen phosphate × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q4A Outward-facing, open1 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain S 1–99（99 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
9SMF Reduced bovine complex I in lipid nanodisc, NADH-active-Q10 Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain S 1–99（99 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.51 Å
9SMG Reduced bovine complex I in lipid nanodisc, NADH-active-altQ10 Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain S 1–99（99 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.42 Å
9SMH Reduced bovine complex I in lipid nanodisc, NADH-active-DDM Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain S 1–99（99 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.61 Å
9SMI Reduced bovine complex I in lipid nanodisc, NADH-deactive Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain S 1–99（99 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 27 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 LMT DODECYL-BETA-D-MALTOSIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 CHD CHOLIC ACID × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.01 Å
9W2U Cryo-EM structure of complex I on the bovine heart submitochondrial particles, open Deposited 2025-07-28 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain S 13–99（87 aa）
Not recorded FME N-FORMYLMETHIONINE × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å

49 other PDB entries and 49 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUA2_BOVIN
Isoform —
PDB entities 19
Chains and sequence ranges Author chain S； PDBConstruct 1–87； UniProt 13–99

Acyl carrier protein, mitochondrial

Bos taurus

UniProt Q9CR21

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain T； UniProt 72–156 Not recorded NADH-ubiquinone oxidoreductase chain 3 × 1 （Q8WAB4） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （P42026） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （P23709） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （P17694） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P04394） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P25708） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P15690） NADH-ubiquinone oxidoreductase chain 1 × 1 （Q6EMT1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （P42028） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03924） NADH-ubiquinone oxidoreductase chain 4L × 1 （Q6EMS3） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03920） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03910） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03892） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （P34942） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （P34943） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q02375） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P23934） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q02370） Acyl carrier protein, mitochondrial × 1 （P52505） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （P23935） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q02366） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P42029） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q8HXG6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q95KV7） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （Q02377） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q02371） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q02376） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q02827） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q02379） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （Q02378） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q8HXG5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q02380） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q02367） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q02374） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q02365） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q02372） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （P48305） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q02369） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q02368） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q02373） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （O97725） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q05752） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） FME N-FORMYLMETHIONINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 2.80 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9W2V Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
6G2J Mouse mitochondrial complex I in the active state Deposited 2018-03-23 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 CDL CARDIOLIPIN × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.30 Å
6G72 Mouse mitochondrial complex I in the deactive state Deposited 2018-04-04 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ACE ACETYL GROUP × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting.
Resolution 3.90 Å
6ZR2 Cryo-EM structure of respiratory complex I in the active state from Mus musculus at 3.1 A Deposited 2020-07-10 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.10 Å
6ZTQ Cryo-EM structure of respiratory complex I from Mus musculus inhibited by piericidin A at 3.0 A Deposited 2020-07-20 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 HQH Piericidin A × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.00 Å
7AK5 Cryo-EM structure of respiratory complex I in the deactive state from Mus musculus at 3.2 A Deposited 2020-09-29 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 8 CDL CARDIOLIPIN × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；8-16s blotting
Resolution 3.17 Å
7AK6 Cryo-EM structure of ND6-P25L mutant respiratory complex I from Mus musculus at 3.8 A Deposited 2020-09-29 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.82 Å
7B93 Cryo-EM structure of mitochondrial complex I from Mus musculus inhibited by IACS-2858 at 3.0 A Deposited 2020-12-14 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 T2Q 1-[[3-(4-methylsulfonylpiperidin-1-yl)phenyl]methyl]-5-[3-[4-(trifluoromethyloxy)phenyl]-1,2,4-oxadiazol-5-yl]pyridin-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.04 Å
7PSA The acetogenin-bound complex I of Mus musculus resolved to 3.4 angstroms Deposited 2021-09-22 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 88I (3~{S},5~{S})-5-methyl-3-[(13~{R})-13-oxidanyl-13-[(2~{R},5~{R})-5-[(2~{R},5~{R})-5-[(1~{R})-1-oxidanylundecyl]oxolan-2-yl]oxolan-2-yl]tridecyl]oxolan-2-one × 1 CDL CARDIOLIPIN × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55；pH 7.55 at 25 degrees Celsius
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8C2S Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 1). Deposited 2022-12-22 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8CA3 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 2). Deposited 2023-01-24 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8CA5 Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 3). Deposited 2023-01-24 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 43 PDB declaration: 43-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 LMT DODECYL-BETA-D-MALTOSIDE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；pH was corrected at room temperature ~22 C
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8IAO Respiratory complex CI:CIII2, type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 12 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IAP Respiratory complex Peripheral Arm of CI, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain T 1–156（156 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8IAQ Respiratory complex Membrane domain of CI, focused map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-02-09 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain U 1–156（156 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8IB4 Respiratory complex CI:CIII2, type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IB5 Respiratory complex Peripheral Arm of CI, focus-refined map of type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain T 1–156（156 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IB6 Respiratory complex Membrane domain of CI, focus-refined of type IA, Wild type mouse under cold temperature Deposited 2023-02-09 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain U 1–156（156 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 CDL CARDIOLIPIN × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IB9 Respiratory complex CI:CIII2, type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 8 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 U10 UBIQUINONE-10 × 2 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8IBA Respiratory complex Peripheral Arm of CI, focus-refined map of type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain T 1–156（156 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8IBB Respiratory complex Membrane domain of CI, focus-refined of type IB, Wild type mouse under cold temperature Deposited 2023-02-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain U 1–156（156 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 CDL CARDIOLIPIN × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IBD Respiratory complex CI:CIII2, type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 67 PDB declaration: 67-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8IBE Respiratory complex Peripheral Arm of CI, focus-refined map of type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain T 1–156（156 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IBF Respiratory complex Membrane domain of CI, focus-refined of type II, Wild type mouse under cold temperature Deposited 2023-02-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain U 1–156（156 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 3 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8IC2 Respiratory complex CI:CIII2, type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 66 PDB declaration: 66-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 UQ6 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL × 2 HEC HEME C × 2 U10 UBIQUINONE-10 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.30 Å
8IC3 Respiratory complex Peripheral Arm of CI, focus-refined map of type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain T 1–156（156 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8IC4 Respiratory complex Membrane domain of CI, focus-refined of type I, PERK -/- mouse under cold temperature Deposited 2023-02-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain U 1–156（156 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 CDL CARDIOLIPIN × 5 ZN ZINC ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8OLT Mitochondrial complex I from Mus musculus in the active state bound with piericidin A Deposited 2023-03-30 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 HQH Piericidin A × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14；PH corrected at room temperature
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 to 12 seconds before plunging
Resolution 2.84 Å
8OM1 Mitochondrial complex I from Mus musculus in the active state Deposited 2023-03-31 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NA SODIUM ION × 1 CDL CARDIOLIPIN × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 16 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.39 Å
8PW5 CS respirasome from murine liver Deposited 2023-07-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 92 PDB declaration: 92-meric
Chain T1 1–156（156 aa）
Chain U1 1–156（156 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 41 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 13 CDL CARDIOLIPIN × 18 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 CU COPPER (II) ION × 2 NA SODIUM ION × 2 HEA HEME-A × 4 MG MAGNESIUM ION × 3 CUA DINUCLEAR COPPER ION × 2 ZN ZINC ION × 3 TGL TRISTEAROYLGLYCEROL × 2 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8PW6 C respirasome from murine liver Deposited 2023-07-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 79 PDB declaration: 79-meric
Chain T1 1–156（156 aa）
Chain U1 1–156（156 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8PW7 A respirasome from murine liver Deposited 2023-07-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 79 PDB declaration: 79-meric
Chain T1 1–156（156 aa）
Chain U1 1–156（156 aa）
Not recorded CU COPPER (II) ION × 1 NA SODIUM ION × 1 HEA HEME-A × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 33 MG MAGNESIUM ION × 2 CUA DINUCLEAR COPPER ION × 1 ZN ZINC ION × 2 TGL TRISTEAROYLGLYCEROL × 1 CDL CARDIOLIPIN × 16 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8RGP Closed Complex I from murine brain Deposited 2023-12-14 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 9 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGQ Open Complex I from murine liver Deposited 2023-12-14 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RGR Closed Complex I from murine liver Deposited 2023-12-14 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 10 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8RGT Open Complex I from murine brain Deposited 2023-12-14 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 2 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8UCA Formation of I2+III2 supercomplex rescues respiratory chain defects Deposited 2023-09-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 111 PDB declaration: 111-meric
Chain AB 1–156（156 aa）
Chain AC 1–156（156 aa）
Chain ab 1–156（156 aa）
Chain ac 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 ZN ZINC ION × 2 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FMN FLAVIN MONONUCLEOTIDE × 2 SF4 IRON/SULFUR CLUSTER × 12 FES FE2/S2 (INORGANIC) CLUSTER × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
8XNL Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-12-30 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain T 1–156（156 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8XNM Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-12-30 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain T 1–156（156 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNN Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type I, Wild type mouse under thermoneutral temperature Deposited 2023-12-30 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain T 1–156（156 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8XNO Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type IA, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain T 1–156（156 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8XNP Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type IA, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain T 1–156（156 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNQ Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type IA, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain T 1–156（156 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 UQ1 UBIQUINONE-1 × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
8XNR Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain T 1–156（156 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8XNS Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain T 1–156（156 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNT Respiratory complex Peripheral Arm of CI, close form C, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain T 1–156（156 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
8XNU Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type IB, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain T 1–156（156 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8XNV Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain T 1–156（156 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 UQ1 UBIQUINONE-1 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8XNW Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain T 1–156（156 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8XNX Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain T 1–156（156 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 UQ9 Ubiquinone-9 × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8XNY Respiratory complex Peripheral Arm of CI, open form B, focus-refined map of type II, Wild type mouse under Cold Acclimation Deposited 2023-12-30 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain T 1–156（156 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
8XNZ Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type I, PERK -/- mouse under Cold Acclimation Deposited 2023-12-30 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain T 1–156（156 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 UQ9 Ubiquinone-9 × 2 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8XO0 Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type I, PERK -/- mouse under Cold Acclimation Deposited 2023-12-30 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 23 PDB declaration: 23-meric
Chain T 1–156（156 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 CDL CARDIOLIPIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
9E9C Mouse mitoribosome large subunit assembly intermediate bound to NSUN4, METRF4, GTPBP7, GTPBP10 and the MALSU1-L0R8F8-mt-ACP complex (without uL16m), State B1 (SAMC knock-out) Deposited 2024-11-08 Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 58-meric
Chain w 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 95 ZN ZINC ION × 2 FES FE2/S2 (INORGANIC) CLUSTER × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.62 Å
9HCC Mouse mitoribosome large subunit assembly intermediate (without uL16m) bound to MRM3-dimer, DDX28 and the MALSU-L0R8F8-mt-ACP complex, State A1 (SAMC knock-out) Deposited 2024-11-08 Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 49 PDB declaration: 51-meric
Chain w 1–156（156 aa）
Not recorded ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.67 Å
9HCD Mouse mitoribosome large subunit assembly intermediate (with uL16m) bound to MRM3-dimer, DDX28 and the MALSU-L0R8F8-mt-ACP complex, state A2 (SAMC knock-out) Deposited 2024-11-08 Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 50 PDB declaration: 52-meric
Chain w 1–156（156 aa）
Not recorded ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.63 Å
9HCE Mouse mitoribosome large subunit assembly intermediate (without uL16m) bound to MRM3 dimer and the MALSU-L0R8F8-mt-ACP complex, State A3 (SAMC knock out) Deposited 2024-11-08 Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 49 PDB declaration: 51-meric
Chain w 1–156（156 aa）
Not recorded ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 7.31 Å
9HCF Mouse mitoribosome large subunit assembly intermediate bound to NSUN4, METRF4, GTPBP7, GTPBP10 and the MALSU-L0R8F8-mtACP complex with uL16m, State B2 (SAMC knock-out) Deposited 2024-11-08 Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 57 PDB declaration: 59-meric
Chain w 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 99 FES FE2/S2 (INORGANIC) CLUSTER × 1 ZN ZINC ION × 2 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.85 Å
9HCG Mouse mitoribosome large subunit assembly intermediate bound to NSUN4, METRF4, MRM2, GTPBP7 and MALSU1-L0R8F8-mt-ACP complex, State D (SAMC knock-out) Deposited 2024-11-08 Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 57 PDB declaration: 59-meric
Chain w 74–152（79 aa）
Not recorded MG MAGNESIUM ION × 105 ZN ZINC ION × 2 FES FE2/S2 (INORGANIC) CLUSTER × 1 SAH S-ADENOSYL-L-HOMOCYSTEINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.91 Å
9HCH Mouse mitoribosome large subunit assembly intermediate bound to NSUN4, METRF4, GTPBP7 and the MALSU1-L0R8F8-mt-ACP complex, State C1 (SAMC knock-out) Deposited 2024-11-10 Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 56 PDB declaration: 58-meric
Chain w 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 99 ZN ZINC ION × 2 FES FE2/S2 (INORGANIC) CLUSTER × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.85 Å
9W2U Cryo-EM structure of complex I on the bovine heart submitochondrial particles, open Deposited 2025-07-28 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 72–156（85 aa）
Not recorded FME N-FORMYLMETHIONINE × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å

60 other PDB entries and 60 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name ACPM_MOUSE
Isoform —
PDB entities 20
Chains and sequence ranges Author chain T； PDBConstruct 1–85； UniProt 72–156

Acyl carrier protein, mitochondrial

Bos taurus

UniProt P52505

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain U； UniProt 71–156 Not recorded NADH-ubiquinone oxidoreductase chain 3 × 1 （Q8WAB4） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （P42026） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （P23709） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （P17694） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P04394） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P25708） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P15690） NADH-ubiquinone oxidoreductase chain 1 × 1 （Q6EMT1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （P42028） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03924） NADH-ubiquinone oxidoreductase chain 4L × 1 （Q6EMS3） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03920） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03910） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03892） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （P34942） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （P34943） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q02375） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P23934） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q02370） Acyl carrier protein, mitochondrial × 1 （Q9CR21） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （P23935） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q02366） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P42029） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q8HXG6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q95KV7） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （Q02377） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q02371） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q02376） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q02827） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q02379） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （Q02378） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q8HXG5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q02380） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q02367） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q02374） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q02365） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q02372） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （P48305） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q02369） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q02368） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q02373） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （O97725） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q05752） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） FME N-FORMYLMETHIONINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 2.80 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9W2V Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5LC5 Structure of mammalian respiratory Complex I, class2 Deposited 2016-06-19 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 76–150（75 aa）
Chain U 72–156（85 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 4.35 Å
5LDW Structure of mammalian respiratory Complex I, class1 Deposited 2016-06-28 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 69–156（88 aa）
Chain U 69–156（88 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus present in a cold room, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 4.27 Å
5LDX Structure of mammalian respiratory Complex I, class3. Deposited 2016-06-28 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 69–156（88 aa）
Chain U 69–156（88 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer 20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7；pH 8
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 5.60 Å
5O31 Mitochondrial complex I in the deactive state Deposited 2017-05-23 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 69–156（88 aa）
Chain U 69–156（88 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.13 Å
7DGQ Activity optimized supercomplex state1 Deposited 2020-11-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain M 71–156（86 aa）
Chain W 71–156（86 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 CU COPPER (II) ION × 3 HEA HEME-A × 2 MG MAGNESIUM ION × 1 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 UQ2 UBIQUINONE-2 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.00 Å
7DGR Activity optimized supercomplex state2 Deposited 2020-11-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain M 69–156（88 aa）
Chain W 69–156（88 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 3 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.60 Å
7DGS Activity optimized supercomplex state3 Deposited 2020-11-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain M 69–156（88 aa）
Chain W 69–156（88 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 7.80 Å
7DGZ Activity optimized complex I (closed form) Deposited 2020-11-12 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain M 69–156（88 aa）
Chain W 69–156（88 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
7DH0 Activity optimized complex I (open form) Deposited 2020-11-12 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain M 69–156（88 aa）
Chain W 69–156（88 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
7DKF Activity optimized supercomplex state4 Deposited 2020-11-24 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain M2 69–156（88 aa）
Chain W2 69–156（88 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 8.30 Å
7QSD Bovine complex I in the active state at 3.1 A Deposited 2022-01-13 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 4 LMT DODECYL-BETA-D-MALTOSIDE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7QSK Bovine complex I in lipid nanodisc, Active-Q10 Deposited 2022-01-13 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.84 Å
7QSL Bovine complex I in lipid nanodisc, Active-apo Deposited 2022-01-13 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.76 Å
7QSM Bovine complex I in lipid nanodisc, Deactive-ligand (composite) Deposited 2022-01-13 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 GOL GLYCEROL × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.30 Å
7QSN Bovine complex I in lipid nanodisc, Deactive-apo Deposited 2022-01-13 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.81 Å
7QSO Bovine complex I in lipid nanodisc, State 3 (Slack) Deposited 2022-01-13 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 6 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 2 CDL CARDIOLIPIN × 6 U10 UBIQUINONE-10 × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.02 Å
7R41 Bovine complex I in the presence of IM1761092, active class i (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R42 Bovine complex I in the presence of IM1761092, active class ii (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R43 Bovine complex I in the presence of IM1761092, active class iii (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 CDL CARDIOLIPIN × 5 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R44 Bovine complex I in the presence of IM1761092, active class iv (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R45 Bovine complex I in the presence of IM1761092, deactive class i (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R46 Bovine complex I in the presence of IM1761092, deactive class ii (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 11 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R47 Bovine complex I in the presence of IM1761092, deactive class iii (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R48 Bovine complex I in the presence of IM1761092, deactive class iv (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4C Bovine complex I in the presence of IM1761092, deactive class v (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4D Bovine complex I in the presence of IM1761092, deactive class vi (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4F Bovine complex I in the presence of IM1761092, slack class i (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 7 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 CDL CARDIOLIPIN × 6 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R4G Bovine complex I in the presence of IM1761092, slack class ii (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 10 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8Q0A Inward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 8 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0F Inward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 21 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0J Inward-facing, slack proteoliposome complex I at 3.8 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.80 Å
8Q0M Outward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0O Outward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0Q Outward-facing, slack proteoliposome complex I at 3.6 A. Initially purified in DDM Deposited 2023-07-28 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.60 Å
8Q1P Inward-facing, open2 proteoliposome complex I at 2.9 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q1U Inward-facing, open1 proteoliposome complex I at 3.3 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.30 Å
8Q1Y Outward-facing, open2 proteoliposome complex I at 2.6 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q25 Outward-facing, open1 proteoliposome complex I at 2.8 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.80 Å
8Q45 Inward-facing, closed proteoliposome complex I at 2.7 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.70 Å
8Q46 Inward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q47 Inward-facing, open1 proteoliposome complex I at 2.9 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 26 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q48 Outward-facing, closed proteoliposome complex I at 2.5 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 35 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.50 Å
8Q49 Outward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 FVH [[(2~{R},3~{S},4~{R},5~{R})-5-[(3~{R})-3-aminocarbonylpiperidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2~{R},3~{R},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3-oxidanyl-4-phosphonooxy-oxolan-2-yl]methyl hydrogen phosphate × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q4A Outward-facing, open1 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
9SMF Reduced bovine complex I in lipid nanodisc, NADH-active-Q10 Deposited 2025-09-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.51 Å
9SMG Reduced bovine complex I in lipid nanodisc, NADH-active-altQ10 Deposited 2025-09-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.42 Å
9SMH Reduced bovine complex I in lipid nanodisc, NADH-active-DDM Deposited 2025-09-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.61 Å
9SMI Reduced bovine complex I in lipid nanodisc, NADH-deactive Deposited 2025-09-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 1–156（156 aa）
Chain U 1–156（156 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 27 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 LMT DODECYL-BETA-D-MALTOSIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 CHD CHOLIC ACID × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.01 Å
9W2U Cryo-EM structure of complex I on the bovine heart submitochondrial particles, open Deposited 2025-07-28 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain U 71–156（86 aa）
Not recorded FME N-FORMYLMETHIONINE × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å

49 other PDB entries and 49 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name ACPM_BOVIN
Isoform —
PDB entities 21
Chains and sequence ranges Author chain U； PDBConstruct 1–86； UniProt 71–156

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5

Bos taurus

UniProt P23935

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain V； UniProt 2–116 Not recorded NADH-ubiquinone oxidoreductase chain 3 × 1 （Q8WAB4） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （P42026） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （P23709） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （P17694） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P04394） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P25708） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P15690） NADH-ubiquinone oxidoreductase chain 1 × 1 （Q6EMT1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （P42028） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03924） NADH-ubiquinone oxidoreductase chain 4L × 1 （Q6EMS3） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03920） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03910） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03892） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （P34942） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （P34943） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q02375） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P23934） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q02370） Acyl carrier protein, mitochondrial × 1 （Q9CR21） Acyl carrier protein, mitochondrial × 1 （P52505） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q02366） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P42029） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q8HXG6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q95KV7） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （Q02377） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q02371） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q02376） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q02827） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q02379） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （Q02378） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q8HXG5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q02380） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q02367） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q02374） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q02365） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q02372） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （P48305） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q02369） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q02368） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q02373） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （O97725） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q05752） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） FME N-FORMYLMETHIONINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 2.80 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9W2V Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5LC5 Structure of mammalian respiratory Complex I, class2 Deposited 2016-06-19 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain V 1–116（116 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 4.35 Å
5LDW Structure of mammalian respiratory Complex I, class1 Deposited 2016-06-28 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain V 2–116（115 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus present in a cold room, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 4.27 Å
5LDX Structure of mammalian respiratory Complex I, class3. Deposited 2016-06-28 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain V 2–116（115 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer 20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7；pH 8
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 5.60 Å
5O31 Mitochondrial complex I in the deactive state Deposited 2017-05-23 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain V 2–116（115 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.13 Å
7DGQ Activity optimized supercomplex state1 Deposited 2020-11-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain N 2–116（115 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 CU COPPER (II) ION × 3 HEA HEME-A × 2 MG MAGNESIUM ION × 1 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 UQ2 UBIQUINONE-2 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.00 Å
7DGR Activity optimized supercomplex state2 Deposited 2020-11-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain N 2–116（115 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 3 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.60 Å
7DGS Activity optimized supercomplex state3 Deposited 2020-11-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain N 2–116（115 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 7.80 Å
7DGZ Activity optimized complex I (closed form) Deposited 2020-11-12 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain N 2–116（115 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
7DH0 Activity optimized complex I (open form) Deposited 2020-11-12 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain N 2–116（115 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
7DKF Activity optimized supercomplex state4 Deposited 2020-11-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain N2 2–116（115 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 8.30 Å
7QSD Bovine complex I in the active state at 3.1 A Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain V 1–116（116 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 4 LMT DODECYL-BETA-D-MALTOSIDE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7QSK Bovine complex I in lipid nanodisc, Active-Q10 Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain V 1–116（116 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.84 Å
7QSL Bovine complex I in lipid nanodisc, Active-apo Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain V 1–116（116 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.76 Å
7QSM Bovine complex I in lipid nanodisc, Deactive-ligand (composite) Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain V 1–116（116 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 GOL GLYCEROL × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.30 Å
7QSN Bovine complex I in lipid nanodisc, Deactive-apo Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain V 1–116（116 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.81 Å
7QSO Bovine complex I in lipid nanodisc, State 3 (Slack) Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain V 1–116（116 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 6 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 2 CDL CARDIOLIPIN × 6 U10 UBIQUINONE-10 × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.02 Å
7R41 Bovine complex I in the presence of IM1761092, active class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain V 1–116（116 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R42 Bovine complex I in the presence of IM1761092, active class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain V 1–116（116 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R43 Bovine complex I in the presence of IM1761092, active class iii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain V 1–116（116 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 CDL CARDIOLIPIN × 5 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R44 Bovine complex I in the presence of IM1761092, active class iv (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain V 1–116（116 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R45 Bovine complex I in the presence of IM1761092, deactive class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain V 1–116（116 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R46 Bovine complex I in the presence of IM1761092, deactive class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain V 1–116（116 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 11 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R47 Bovine complex I in the presence of IM1761092, deactive class iii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain V 1–116（116 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R48 Bovine complex I in the presence of IM1761092, deactive class iv (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain V 1–116（116 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4C Bovine complex I in the presence of IM1761092, deactive class v (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain V 1–116（116 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4D Bovine complex I in the presence of IM1761092, deactive class vi (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain V 1–116（116 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4F Bovine complex I in the presence of IM1761092, slack class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain V 1–116（116 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 7 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 CDL CARDIOLIPIN × 6 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R4G Bovine complex I in the presence of IM1761092, slack class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain V 1–116（116 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 10 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8Q0A Inward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain V 1–116（116 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 8 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0F Inward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain V 1–116（116 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 21 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0J Inward-facing, slack proteoliposome complex I at 3.8 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain V 1–116（116 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.80 Å
8Q0M Outward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain V 1–116（116 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0O Outward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain V 1–116（116 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0Q Outward-facing, slack proteoliposome complex I at 3.6 A. Initially purified in DDM Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain V 1–116（116 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.60 Å
8Q1P Inward-facing, open2 proteoliposome complex I at 2.9 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain V 1–116（116 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q1U Inward-facing, open1 proteoliposome complex I at 3.3 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain V 1–116（116 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.30 Å
8Q1Y Outward-facing, open2 proteoliposome complex I at 2.6 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain V 1–116（116 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q25 Outward-facing, open1 proteoliposome complex I at 2.8 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain V 1–116（116 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.80 Å
8Q45 Inward-facing, closed proteoliposome complex I at 2.7 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain V 1–116（116 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.70 Å
8Q46 Inward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain V 1–116（116 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q47 Inward-facing, open1 proteoliposome complex I at 2.9 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain V 1–116（116 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 26 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q48 Outward-facing, closed proteoliposome complex I at 2.5 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain V 1–116（116 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 35 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.50 Å
8Q49 Outward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain V 1–116（116 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 FVH [[(2~{R},3~{S},4~{R},5~{R})-5-[(3~{R})-3-aminocarbonylpiperidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2~{R},3~{R},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3-oxidanyl-4-phosphonooxy-oxolan-2-yl]methyl hydrogen phosphate × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q4A Outward-facing, open1 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain V 1–116（116 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
9SMF Reduced bovine complex I in lipid nanodisc, NADH-active-Q10 Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain V 1–116（116 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.51 Å
9SMG Reduced bovine complex I in lipid nanodisc, NADH-active-altQ10 Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain V 1–116（116 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.42 Å
9SMH Reduced bovine complex I in lipid nanodisc, NADH-active-DDM Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain V 1–116（116 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.61 Å
9SMI Reduced bovine complex I in lipid nanodisc, NADH-deactive Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain V 1–116（116 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 27 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 LMT DODECYL-BETA-D-MALTOSIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 CHD CHOLIC ACID × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.01 Å
9W2U Cryo-EM structure of complex I on the bovine heart submitochondrial particles, open Deposited 2025-07-28 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain V 2–116（115 aa）
Not recorded FME N-FORMYLMETHIONINE × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å

49 other PDB entries and 49 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUA5_BOVIN
Isoform —
PDB entities 22
Chains and sequence ranges Author chain V； PDBConstruct 1–115； UniProt 2–116

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6

Bos taurus

UniProt Q02366

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain W； UniProt 14–128 Not recorded NADH-ubiquinone oxidoreductase chain 3 × 1 （Q8WAB4） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （P42026） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （P23709） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （P17694） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P04394） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P25708） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P15690） NADH-ubiquinone oxidoreductase chain 1 × 1 （Q6EMT1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （P42028） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03924） NADH-ubiquinone oxidoreductase chain 4L × 1 （Q6EMS3） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03920） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03910） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03892） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （P34942） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （P34943） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q02375） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P23934） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q02370） Acyl carrier protein, mitochondrial × 1 （Q9CR21） Acyl carrier protein, mitochondrial × 1 （P52505） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （P23935） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P42029） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q8HXG6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q95KV7） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （Q02377） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q02371） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q02376） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q02827） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q02379） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （Q02378） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q8HXG5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q02380） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q02367） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q02374） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q02365） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q02372） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （P48305） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q02369） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q02368） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q02373） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （O97725） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q05752） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） FME N-FORMYLMETHIONINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 2.80 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9W2V Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5LC5 Structure of mammalian respiratory Complex I, class2 Deposited 2016-06-19 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain W 1–128（128 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 4.35 Å
5LDW Structure of mammalian respiratory Complex I, class1 Deposited 2016-06-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain W 1–128（128 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus present in a cold room, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 4.27 Å
5LDX Structure of mammalian respiratory Complex I, class3. Deposited 2016-06-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain W 2–128（127 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer 20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7；pH 8
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 5.60 Å
5O31 Mitochondrial complex I in the deactive state Deposited 2017-05-23 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain W 1–128（128 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.13 Å
7DGQ Activity optimized supercomplex state1 Deposited 2020-11-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain O 2–128（127 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 CU COPPER (II) ION × 3 HEA HEME-A × 2 MG MAGNESIUM ION × 1 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 UQ2 UBIQUINONE-2 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.00 Å
7DGR Activity optimized supercomplex state2 Deposited 2020-11-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain O 2–128（127 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 3 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.60 Å
7DGS Activity optimized supercomplex state3 Deposited 2020-11-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain O 2–128（127 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 7.80 Å
7DGZ Activity optimized complex I (closed form) Deposited 2020-11-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain O 2–128（127 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
7DH0 Activity optimized complex I (open form) Deposited 2020-11-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain O 2–128（127 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
7DKF Activity optimized supercomplex state4 Deposited 2020-11-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain O2 2–128（127 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 8.30 Å
7QSD Bovine complex I in the active state at 3.1 A Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain W 1–128（128 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 4 LMT DODECYL-BETA-D-MALTOSIDE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7QSK Bovine complex I in lipid nanodisc, Active-Q10 Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain W 1–128（128 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.84 Å
7QSL Bovine complex I in lipid nanodisc, Active-apo Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain W 1–128（128 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.76 Å
7QSM Bovine complex I in lipid nanodisc, Deactive-ligand (composite) Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain W 1–128（128 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 GOL GLYCEROL × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.30 Å
7QSN Bovine complex I in lipid nanodisc, Deactive-apo Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain W 1–128（128 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.81 Å
7QSO Bovine complex I in lipid nanodisc, State 3 (Slack) Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain W 1–128（128 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 6 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 2 CDL CARDIOLIPIN × 6 U10 UBIQUINONE-10 × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.02 Å
7R41 Bovine complex I in the presence of IM1761092, active class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain W 1–128（128 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R42 Bovine complex I in the presence of IM1761092, active class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain W 1–128（128 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R43 Bovine complex I in the presence of IM1761092, active class iii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain W 1–128（128 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 CDL CARDIOLIPIN × 5 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R44 Bovine complex I in the presence of IM1761092, active class iv (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain W 1–128（128 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R45 Bovine complex I in the presence of IM1761092, deactive class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain W 1–128（128 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R46 Bovine complex I in the presence of IM1761092, deactive class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain W 1–128（128 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 11 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R47 Bovine complex I in the presence of IM1761092, deactive class iii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain W 1–128（128 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R48 Bovine complex I in the presence of IM1761092, deactive class iv (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain W 1–128（128 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4C Bovine complex I in the presence of IM1761092, deactive class v (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain W 1–128（128 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4D Bovine complex I in the presence of IM1761092, deactive class vi (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain W 1–128（128 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4F Bovine complex I in the presence of IM1761092, slack class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain W 1–128（128 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 7 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 CDL CARDIOLIPIN × 6 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R4G Bovine complex I in the presence of IM1761092, slack class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain W 1–128（128 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 10 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8Q0A Inward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain W 1–128（128 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 8 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0F Inward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain W 1–128（128 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 21 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0J Inward-facing, slack proteoliposome complex I at 3.8 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain W 1–128（128 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.80 Å
8Q0M Outward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain W 1–128（128 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0O Outward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain W 1–128（128 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0Q Outward-facing, slack proteoliposome complex I at 3.6 A. Initially purified in DDM Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain W 1–128（128 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.60 Å
8Q1P Inward-facing, open2 proteoliposome complex I at 2.9 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain W 1–128（128 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q1U Inward-facing, open1 proteoliposome complex I at 3.3 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain W 1–128（128 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.30 Å
8Q1Y Outward-facing, open2 proteoliposome complex I at 2.6 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain W 1–128（128 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q25 Outward-facing, open1 proteoliposome complex I at 2.8 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain W 1–128（128 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.80 Å
8Q45 Inward-facing, closed proteoliposome complex I at 2.7 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain W 1–128（128 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.70 Å
8Q46 Inward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain W 1–128（128 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q47 Inward-facing, open1 proteoliposome complex I at 2.9 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain W 1–128（128 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 26 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q48 Outward-facing, closed proteoliposome complex I at 2.5 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain W 1–128（128 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 35 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.50 Å
8Q49 Outward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain W 1–128（128 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 FVH [[(2~{R},3~{S},4~{R},5~{R})-5-[(3~{R})-3-aminocarbonylpiperidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2~{R},3~{R},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3-oxidanyl-4-phosphonooxy-oxolan-2-yl]methyl hydrogen phosphate × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q4A Outward-facing, open1 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain W 1–128（128 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
9SMF Reduced bovine complex I in lipid nanodisc, NADH-active-Q10 Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain W 1–128（128 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.51 Å
9SMG Reduced bovine complex I in lipid nanodisc, NADH-active-altQ10 Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain W 1–128（128 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.42 Å
9SMH Reduced bovine complex I in lipid nanodisc, NADH-active-DDM Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain W 1–128（128 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.61 Å
9SMI Reduced bovine complex I in lipid nanodisc, NADH-deactive Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain W 1–128（128 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 27 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 LMT DODECYL-BETA-D-MALTOSIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 CHD CHOLIC ACID × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.01 Å
9W2U Cryo-EM structure of complex I on the bovine heart submitochondrial particles, open Deposited 2025-07-28 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain W 14–128（115 aa）
Not recorded FME N-FORMYLMETHIONINE × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å

49 other PDB entries and 49 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUA6_BOVIN
Isoform —
PDB entities 23
Chains and sequence ranges Author chain W； PDBConstruct 1–115； UniProt 14–128

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8

Bos taurus

UniProt P42029

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain X； UniProt 2–172 Not recorded NADH-ubiquinone oxidoreductase chain 3 × 1 （Q8WAB4） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （P42026） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （P23709） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （P17694） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P04394） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P25708） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P15690） NADH-ubiquinone oxidoreductase chain 1 × 1 （Q6EMT1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （P42028） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03924） NADH-ubiquinone oxidoreductase chain 4L × 1 （Q6EMS3） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03920） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03910） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03892） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （P34942） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （P34943） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q02375） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P23934） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q02370） Acyl carrier protein, mitochondrial × 1 （Q9CR21） Acyl carrier protein, mitochondrial × 1 （P52505） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （P23935） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q02366） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q8HXG6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q95KV7） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （Q02377） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q02371） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q02376） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q02827） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q02379） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （Q02378） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q8HXG5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q02380） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q02367） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q02374） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q02365） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q02372） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （P48305） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q02369） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q02368） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q02373） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （O97725） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q05752） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） FME N-FORMYLMETHIONINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 2.80 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9W2V Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5LC5 Structure of mammalian respiratory Complex I, class2 Deposited 2016-06-19 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain X 1–114（114 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 4.35 Å
5LDW Structure of mammalian respiratory Complex I, class1 Deposited 2016-06-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain X 1–114（114 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus present in a cold room, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 4.27 Å
5LDX Structure of mammalian respiratory Complex I, class3. Deposited 2016-06-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain X 6–114（109 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer 20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7；pH 8
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 5.60 Å
5O31 Mitochondrial complex I in the deactive state Deposited 2017-05-23 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain X 2–172（171 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.13 Å
7DGQ Activity optimized supercomplex state1 Deposited 2020-11-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain Q 2–172（171 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 CU COPPER (II) ION × 3 HEA HEME-A × 2 MG MAGNESIUM ION × 1 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 UQ2 UBIQUINONE-2 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.00 Å
7DGR Activity optimized supercomplex state2 Deposited 2020-11-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain Q 2–172（171 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 3 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.60 Å
7DGS Activity optimized supercomplex state3 Deposited 2020-11-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain Q 2–172（171 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 7.80 Å
7DGZ Activity optimized complex I (closed form) Deposited 2020-11-12 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Q 2–172（171 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
7DH0 Activity optimized complex I (open form) Deposited 2020-11-12 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Q 2–172（171 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
7DKF Activity optimized supercomplex state4 Deposited 2020-11-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain Q2 2–172（171 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 8.30 Å
7QSD Bovine complex I in the active state at 3.1 A Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain X 1–172（172 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 4 LMT DODECYL-BETA-D-MALTOSIDE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7QSK Bovine complex I in lipid nanodisc, Active-Q10 Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain X 1–172（172 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.84 Å
7QSL Bovine complex I in lipid nanodisc, Active-apo Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain X 1–172（172 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.76 Å
7QSM Bovine complex I in lipid nanodisc, Deactive-ligand (composite) Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain X 1–172（172 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 GOL GLYCEROL × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.30 Å
7QSN Bovine complex I in lipid nanodisc, Deactive-apo Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain X 1–172（172 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.81 Å
7QSO Bovine complex I in lipid nanodisc, State 3 (Slack) Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain X 1–172（172 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 6 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 2 CDL CARDIOLIPIN × 6 U10 UBIQUINONE-10 × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.02 Å
7R41 Bovine complex I in the presence of IM1761092, active class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain X 1–172（172 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R42 Bovine complex I in the presence of IM1761092, active class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain X 1–172（172 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R43 Bovine complex I in the presence of IM1761092, active class iii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain X 1–172（172 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 CDL CARDIOLIPIN × 5 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R44 Bovine complex I in the presence of IM1761092, active class iv (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain X 1–172（172 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R45 Bovine complex I in the presence of IM1761092, deactive class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain X 1–172（172 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R46 Bovine complex I in the presence of IM1761092, deactive class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain X 1–172（172 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 11 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R47 Bovine complex I in the presence of IM1761092, deactive class iii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain X 1–172（172 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R48 Bovine complex I in the presence of IM1761092, deactive class iv (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain X 1–172（172 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4C Bovine complex I in the presence of IM1761092, deactive class v (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain X 1–172（172 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4D Bovine complex I in the presence of IM1761092, deactive class vi (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain X 1–172（172 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4F Bovine complex I in the presence of IM1761092, slack class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain X 1–172（172 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 7 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 CDL CARDIOLIPIN × 6 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R4G Bovine complex I in the presence of IM1761092, slack class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain X 1–172（172 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 10 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8Q0A Inward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain X 1–172（172 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 8 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0F Inward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain X 1–172（172 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 21 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0J Inward-facing, slack proteoliposome complex I at 3.8 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain X 1–172（172 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.80 Å
8Q0M Outward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain X 1–172（172 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0O Outward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain X 1–172（172 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0Q Outward-facing, slack proteoliposome complex I at 3.6 A. Initially purified in DDM Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain X 1–172（172 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.60 Å
8Q1P Inward-facing, open2 proteoliposome complex I at 2.9 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain X 1–172（172 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q1U Inward-facing, open1 proteoliposome complex I at 3.3 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain X 1–172（172 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.30 Å
8Q1Y Outward-facing, open2 proteoliposome complex I at 2.6 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain X 1–172（172 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q25 Outward-facing, open1 proteoliposome complex I at 2.8 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain X 1–172（172 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.80 Å
8Q45 Inward-facing, closed proteoliposome complex I at 2.7 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain X 1–172（172 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.70 Å
8Q46 Inward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain X 1–172（172 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q47 Inward-facing, open1 proteoliposome complex I at 2.9 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain X 1–172（172 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 26 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q48 Outward-facing, closed proteoliposome complex I at 2.5 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain X 1–172（172 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 35 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.50 Å
8Q49 Outward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain X 1–172（172 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 FVH [[(2~{R},3~{S},4~{R},5~{R})-5-[(3~{R})-3-aminocarbonylpiperidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2~{R},3~{R},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3-oxidanyl-4-phosphonooxy-oxolan-2-yl]methyl hydrogen phosphate × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q4A Outward-facing, open1 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain X 1–172（172 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
9SMF Reduced bovine complex I in lipid nanodisc, NADH-active-Q10 Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain X 1–172（172 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.51 Å
9SMG Reduced bovine complex I in lipid nanodisc, NADH-active-altQ10 Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain X 1–172（172 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.42 Å
9SMH Reduced bovine complex I in lipid nanodisc, NADH-active-DDM Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain X 1–172（172 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.61 Å
9SMI Reduced bovine complex I in lipid nanodisc, NADH-deactive Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain X 1–172（172 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 27 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 LMT DODECYL-BETA-D-MALTOSIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 CHD CHOLIC ACID × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.01 Å
9W2U Cryo-EM structure of complex I on the bovine heart submitochondrial particles, open Deposited 2025-07-28 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain X 2–172（171 aa）
Not recorded FME N-FORMYLMETHIONINE × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å

49 other PDB entries and 49 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUA8_BOVIN
Isoform —
PDB entities 24
Chains and sequence ranges Author chain X； PDBConstruct 1–171； UniProt 2–172

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11

Bos taurus

UniProt Q8HXG6

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain Y； UniProt 2–141 Non-standard monomer：Yes (specific site not provided by mmCIF) NADH-ubiquinone oxidoreductase chain 3 × 1 （Q8WAB4） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （P42026） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （P23709） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （P17694） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P04394） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P25708） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P15690） NADH-ubiquinone oxidoreductase chain 1 × 1 （Q6EMT1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （P42028） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03924） NADH-ubiquinone oxidoreductase chain 4L × 1 （Q6EMS3） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03920） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03910） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03892） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （P34942） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （P34943） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q02375） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P23934） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q02370） Acyl carrier protein, mitochondrial × 1 （Q9CR21） Acyl carrier protein, mitochondrial × 1 （P52505） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （P23935） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q02366） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P42029） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q95KV7） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （Q02377） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q02371） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q02376） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q02827） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q02379） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （Q02378） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q8HXG5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q02380） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q02367） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q02374） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q02365） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q02372） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （P48305） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q02369） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q02368） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q02373） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （O97725） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q05752） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） FME N-FORMYLMETHIONINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 2.80 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9W2V Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5LC5 Structure of mammalian respiratory Complex I, class2 Deposited 2016-06-19 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Y 2–139（138 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 4.35 Å
5LDW Structure of mammalian respiratory Complex I, class1 Deposited 2016-06-28 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Y 1–141（141 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus present in a cold room, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 4.27 Å
5LDX Structure of mammalian respiratory Complex I, class3. Deposited 2016-06-28 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Y 2–141（140 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer 20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7；pH 8
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 5.60 Å
5O31 Mitochondrial complex I in the deactive state Deposited 2017-05-23 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Y 1–141（141 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.13 Å
7DGQ Activity optimized supercomplex state1 Deposited 2020-11-12 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain T 2–141（140 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 CU COPPER (II) ION × 3 HEA HEME-A × 2 MG MAGNESIUM ION × 1 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 UQ2 UBIQUINONE-2 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.00 Å
7DGR Activity optimized supercomplex state2 Deposited 2020-11-12 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain T 2–141（140 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 3 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.60 Å
7DGS Activity optimized supercomplex state3 Deposited 2020-11-12 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain T 2–141（140 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 7.80 Å
7DGZ Activity optimized complex I (closed form) Deposited 2020-11-12 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 2–141（140 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
7DH0 Activity optimized complex I (open form) Deposited 2020-11-12 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain T 2–141（140 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
7DKF Activity optimized supercomplex state4 Deposited 2020-11-24 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain T2 2–141（140 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 8.30 Å
7QSD Bovine complex I in the active state at 3.1 A Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Y 1–141（141 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 4 LMT DODECYL-BETA-D-MALTOSIDE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7QSK Bovine complex I in lipid nanodisc, Active-Q10 Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Y 1–141（141 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.84 Å
7QSL Bovine complex I in lipid nanodisc, Active-apo Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Y 1–141（141 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.76 Å
7QSM Bovine complex I in lipid nanodisc, Deactive-ligand (composite) Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Y 1–141（141 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 GOL GLYCEROL × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.30 Å
7QSN Bovine complex I in lipid nanodisc, Deactive-apo Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Y 1–141（141 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.81 Å
7QSO Bovine complex I in lipid nanodisc, State 3 (Slack) Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Y 1–141（141 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 6 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 2 CDL CARDIOLIPIN × 6 U10 UBIQUINONE-10 × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.02 Å
7R41 Bovine complex I in the presence of IM1761092, active class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Y 1–141（141 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R42 Bovine complex I in the presence of IM1761092, active class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Y 1–141（141 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R43 Bovine complex I in the presence of IM1761092, active class iii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Y 1–141（141 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 CDL CARDIOLIPIN × 5 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R44 Bovine complex I in the presence of IM1761092, active class iv (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Y 1–141（141 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R45 Bovine complex I in the presence of IM1761092, deactive class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Y 1–141（141 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R46 Bovine complex I in the presence of IM1761092, deactive class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Y 1–141（141 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 11 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R47 Bovine complex I in the presence of IM1761092, deactive class iii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Y 1–141（141 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R48 Bovine complex I in the presence of IM1761092, deactive class iv (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Y 1–141（141 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4C Bovine complex I in the presence of IM1761092, deactive class v (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Y 1–141（141 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4D Bovine complex I in the presence of IM1761092, deactive class vi (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Y 1–141（141 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4F Bovine complex I in the presence of IM1761092, slack class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Y 1–141（141 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 7 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 CDL CARDIOLIPIN × 6 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R4G Bovine complex I in the presence of IM1761092, slack class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Y 1–141（141 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 10 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8Q0A Inward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Y 1–141（141 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 8 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0F Inward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Y 1–141（141 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 21 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0J Inward-facing, slack proteoliposome complex I at 3.8 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Y 1–141（141 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.80 Å
8Q0M Outward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Y 1–141（141 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0O Outward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Y 1–141（141 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0Q Outward-facing, slack proteoliposome complex I at 3.6 A. Initially purified in DDM Deposited 2023-07-28 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Y 1–141（141 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.60 Å
8Q1P Inward-facing, open2 proteoliposome complex I at 2.9 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Y 1–141（141 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q1U Inward-facing, open1 proteoliposome complex I at 3.3 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Y 1–141（141 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.30 Å
8Q1Y Outward-facing, open2 proteoliposome complex I at 2.6 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Y 1–141（141 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q25 Outward-facing, open1 proteoliposome complex I at 2.8 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Y 1–141（141 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.80 Å
8Q45 Inward-facing, closed proteoliposome complex I at 2.7 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Y 1–141（141 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.70 Å
8Q46 Inward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Y 1–141（141 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q47 Inward-facing, open1 proteoliposome complex I at 2.9 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Y 1–141（141 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 26 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q48 Outward-facing, closed proteoliposome complex I at 2.5 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Y 1–141（141 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 35 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.50 Å
8Q49 Outward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Y 1–141（141 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 FVH [[(2~{R},3~{S},4~{R},5~{R})-5-[(3~{R})-3-aminocarbonylpiperidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2~{R},3~{R},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3-oxidanyl-4-phosphonooxy-oxolan-2-yl]methyl hydrogen phosphate × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q4A Outward-facing, open1 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Y 1–141（141 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
9SMF Reduced bovine complex I in lipid nanodisc, NADH-active-Q10 Deposited 2025-09-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Y 2–141（140 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.51 Å
9SMG Reduced bovine complex I in lipid nanodisc, NADH-active-altQ10 Deposited 2025-09-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Y 2–141（140 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.42 Å
9SMH Reduced bovine complex I in lipid nanodisc, NADH-active-DDM Deposited 2025-09-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Y 2–141（140 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.61 Å
9SMI Reduced bovine complex I in lipid nanodisc, NADH-deactive Deposited 2025-09-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Y 2–141（140 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 27 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 LMT DODECYL-BETA-D-MALTOSIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 CHD CHOLIC ACID × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.01 Å
9W2U Cryo-EM structure of complex I on the bovine heart submitochondrial particles, open Deposited 2025-07-28 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain t 2–141（140 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) FME N-FORMYLMETHIONINE × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å

49 other PDB entries and 49 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUAB_BOVIN
Isoform —
PDB entities 25
Chains and sequence ranges Author chain Y； PDBConstruct 2–141； UniProt 2–141

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13

Bos taurus

UniProt Q95KV7

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain Z； UniProt 4–144 Not recorded NADH-ubiquinone oxidoreductase chain 3 × 1 （Q8WAB4） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （P42026） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （P23709） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （P17694） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P04394） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P25708） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P15690） NADH-ubiquinone oxidoreductase chain 1 × 1 （Q6EMT1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （P42028） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03924） NADH-ubiquinone oxidoreductase chain 4L × 1 （Q6EMS3） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03920） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03910） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03892） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （P34942） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （P34943） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q02375） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P23934） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q02370） Acyl carrier protein, mitochondrial × 1 （Q9CR21） Acyl carrier protein, mitochondrial × 1 （P52505） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （P23935） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q02366） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P42029） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q8HXG6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （Q02377） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q02371） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q02376） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q02827） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q02379） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （Q02378） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q8HXG5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q02380） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q02367） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q02374） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q02365） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q02372） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （P48305） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q02369） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q02368） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q02373） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （O97725） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q05752） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） FME N-FORMYLMETHIONINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 2.80 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9W2V Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5LC5 Structure of mammalian respiratory Complex I, class2 Deposited 2016-06-19 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Z 32–100（69 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 4.35 Å
5LDW Structure of mammalian respiratory Complex I, class1 Deposited 2016-06-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Z 32–100（69 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus present in a cold room, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 4.27 Å
5LDX Structure of mammalian respiratory Complex I, class3. Deposited 2016-06-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Z 32–100（69 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer 20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7；pH 8
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 5.60 Å
5O31 Mitochondrial complex I in the deactive state Deposited 2017-05-23 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Z 1–99（99 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.13 Å
7DGQ Activity optimized supercomplex state1 Deposited 2020-11-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain V 2–144（143 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 CU COPPER (II) ION × 3 HEA HEME-A × 2 MG MAGNESIUM ION × 1 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 UQ2 UBIQUINONE-2 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.00 Å
7DGR Activity optimized supercomplex state2 Deposited 2020-11-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain V 2–144（143 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 3 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.60 Å
7DGS Activity optimized supercomplex state3 Deposited 2020-11-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain V 2–144（143 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 7.80 Å
7DGZ Activity optimized complex I (closed form) Deposited 2020-11-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain V 2–144（143 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
7DH0 Activity optimized complex I (open form) Deposited 2020-11-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain V 2–144（143 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
7DKF Activity optimized supercomplex state4 Deposited 2020-11-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain V2 2–144（143 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 8.30 Å
7QSD Bovine complex I in the active state at 3.1 A Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Z 1–144（144 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 4 LMT DODECYL-BETA-D-MALTOSIDE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7QSK Bovine complex I in lipid nanodisc, Active-Q10 Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Z 1–144（144 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.84 Å
7QSL Bovine complex I in lipid nanodisc, Active-apo Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Z 1–144（144 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.76 Å
7QSM Bovine complex I in lipid nanodisc, Deactive-ligand (composite) Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Z 1–144（144 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 GOL GLYCEROL × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.30 Å
7QSN Bovine complex I in lipid nanodisc, Deactive-apo Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Z 1–144（144 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.81 Å
7QSO Bovine complex I in lipid nanodisc, State 3 (Slack) Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Z 1–144（144 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 6 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 2 CDL CARDIOLIPIN × 6 U10 UBIQUINONE-10 × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.02 Å
7R41 Bovine complex I in the presence of IM1761092, active class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Z 1–144（144 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R42 Bovine complex I in the presence of IM1761092, active class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Z 1–144（144 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R43 Bovine complex I in the presence of IM1761092, active class iii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Z 1–144（144 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 CDL CARDIOLIPIN × 5 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R44 Bovine complex I in the presence of IM1761092, active class iv (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Z 1–144（144 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R45 Bovine complex I in the presence of IM1761092, deactive class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Z 1–144（144 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R46 Bovine complex I in the presence of IM1761092, deactive class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Z 1–144（144 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 11 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R47 Bovine complex I in the presence of IM1761092, deactive class iii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Z 1–144（144 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R48 Bovine complex I in the presence of IM1761092, deactive class iv (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Z 1–144（144 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4C Bovine complex I in the presence of IM1761092, deactive class v (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Z 1–144（144 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4D Bovine complex I in the presence of IM1761092, deactive class vi (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Z 1–144（144 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4F Bovine complex I in the presence of IM1761092, slack class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Z 1–144（144 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 7 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 CDL CARDIOLIPIN × 6 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R4G Bovine complex I in the presence of IM1761092, slack class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Z 1–144（144 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 10 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8Q0A Inward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Z 1–144（144 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 8 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0F Inward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Z 1–144（144 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 21 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0J Inward-facing, slack proteoliposome complex I at 3.8 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Z 1–144（144 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.80 Å
8Q0M Outward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Z 1–144（144 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0O Outward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Z 1–144（144 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0Q Outward-facing, slack proteoliposome complex I at 3.6 A. Initially purified in DDM Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Z 1–144（144 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.60 Å
8Q1P Inward-facing, open2 proteoliposome complex I at 2.9 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Z 1–144（144 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q1U Inward-facing, open1 proteoliposome complex I at 3.3 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Z 1–144（144 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.30 Å
8Q1Y Outward-facing, open2 proteoliposome complex I at 2.6 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Z 1–144（144 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q25 Outward-facing, open1 proteoliposome complex I at 2.8 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Z 1–144（144 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.80 Å
8Q45 Inward-facing, closed proteoliposome complex I at 2.7 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Z 1–144（144 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.70 Å
8Q46 Inward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Z 1–144（144 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q47 Inward-facing, open1 proteoliposome complex I at 2.9 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Z 1–144（144 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 26 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q48 Outward-facing, closed proteoliposome complex I at 2.5 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Z 1–144（144 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 35 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.50 Å
8Q49 Outward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Z 1–144（144 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 FVH [[(2~{R},3~{S},4~{R},5~{R})-5-[(3~{R})-3-aminocarbonylpiperidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2~{R},3~{R},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3-oxidanyl-4-phosphonooxy-oxolan-2-yl]methyl hydrogen phosphate × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q4A Outward-facing, open1 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Z 1–144（144 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
9SMF Reduced bovine complex I in lipid nanodisc, NADH-active-Q10 Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Z 1–144（144 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.51 Å
9SMG Reduced bovine complex I in lipid nanodisc, NADH-active-altQ10 Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Z 1–144（144 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.42 Å
9SMH Reduced bovine complex I in lipid nanodisc, NADH-active-DDM Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Z 1–144（144 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.61 Å
9SMI Reduced bovine complex I in lipid nanodisc, NADH-deactive Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Z 1–144（144 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 27 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 LMT DODECYL-BETA-D-MALTOSIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 CHD CHOLIC ACID × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.01 Å
9W2U Cryo-EM structure of complex I on the bovine heart submitochondrial particles, open Deposited 2025-07-28 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Z 4–144（141 aa）
Not recorded FME N-FORMYLMETHIONINE × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å

49 other PDB entries and 49 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUAD_BOVIN
Isoform —
PDB entities 26
Chains and sequence ranges Author chain Z； PDBConstruct 1–141； UniProt 4–144

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1

Bos taurus

UniProt Q02377

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain a； UniProt 1–70 Not recorded NADH-ubiquinone oxidoreductase chain 3 × 1 （Q8WAB4） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （P42026） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （P23709） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （P17694） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P04394） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P25708） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P15690） NADH-ubiquinone oxidoreductase chain 1 × 1 （Q6EMT1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （P42028） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03924） NADH-ubiquinone oxidoreductase chain 4L × 1 （Q6EMS3） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03920） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03910） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03892） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （P34942） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （P34943） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q02375） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P23934） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q02370） Acyl carrier protein, mitochondrial × 1 （Q9CR21） Acyl carrier protein, mitochondrial × 1 （P52505） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （P23935） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q02366） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P42029） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q8HXG6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q95KV7） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q02371） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q02376） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q02827） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q02379） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （Q02378） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q8HXG5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q02380） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q02367） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q02374） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q02365） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q02372） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （P48305） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q02369） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q02368） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q02373） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （O97725） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q05752） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） FME N-FORMYLMETHIONINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 2.80 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9W2V Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5LC5 Structure of mammalian respiratory Complex I, class2 Deposited 2016-06-19 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain a 1–70（70 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 4.35 Å
5LDW Structure of mammalian respiratory Complex I, class1 Deposited 2016-06-28 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain a 1–70（70 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus present in a cold room, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 4.27 Å
5LDX Structure of mammalian respiratory Complex I, class3. Deposited 2016-06-28 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain a 1–70（70 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer 20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7；pH 8
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 5.60 Å
5O31 Mitochondrial complex I in the deactive state Deposited 2017-05-23 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain a 1–70（70 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.13 Å
7DGQ Activity optimized supercomplex state1 Deposited 2020-11-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain J 1–70（70 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 CU COPPER (II) ION × 3 HEA HEME-A × 2 MG MAGNESIUM ION × 1 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 UQ2 UBIQUINONE-2 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.00 Å
7DGR Activity optimized supercomplex state2 Deposited 2020-11-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain J 1–70（70 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 3 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.60 Å
7DGS Activity optimized supercomplex state3 Deposited 2020-11-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain J 1–70（70 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 7.80 Å
7DGZ Activity optimized complex I (closed form) Deposited 2020-11-12 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain J 1–70（70 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
7DH0 Activity optimized complex I (open form) Deposited 2020-11-12 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain J 1–70（70 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
7DKF Activity optimized supercomplex state4 Deposited 2020-11-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain J2 1–70（70 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 8.30 Å
7QSD Bovine complex I in the active state at 3.1 A Deposited 2022-01-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain a 1–70（70 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 4 LMT DODECYL-BETA-D-MALTOSIDE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7QSK Bovine complex I in lipid nanodisc, Active-Q10 Deposited 2022-01-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain a 1–70（70 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.84 Å
7QSL Bovine complex I in lipid nanodisc, Active-apo Deposited 2022-01-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain a 1–70（70 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.76 Å
7QSM Bovine complex I in lipid nanodisc, Deactive-ligand (composite) Deposited 2022-01-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain a 1–70（70 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 GOL GLYCEROL × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.30 Å
7QSN Bovine complex I in lipid nanodisc, Deactive-apo Deposited 2022-01-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain a 1–70（70 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.81 Å
7QSO Bovine complex I in lipid nanodisc, State 3 (Slack) Deposited 2022-01-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain a 1–70（70 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 6 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 2 CDL CARDIOLIPIN × 6 U10 UBIQUINONE-10 × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.02 Å
7R41 Bovine complex I in the presence of IM1761092, active class i (Composite map) Deposited 2022-02-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain a 1–70（70 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R42 Bovine complex I in the presence of IM1761092, active class ii (Composite map) Deposited 2022-02-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain a 1–70（70 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R43 Bovine complex I in the presence of IM1761092, active class iii (Composite map) Deposited 2022-02-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain a 1–70（70 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 CDL CARDIOLIPIN × 5 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R44 Bovine complex I in the presence of IM1761092, active class iv (Composite map) Deposited 2022-02-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain a 1–70（70 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R45 Bovine complex I in the presence of IM1761092, deactive class i (Composite map) Deposited 2022-02-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain a 1–70（70 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R46 Bovine complex I in the presence of IM1761092, deactive class ii (Composite map) Deposited 2022-02-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain a 1–70（70 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 11 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R47 Bovine complex I in the presence of IM1761092, deactive class iii (Composite map) Deposited 2022-02-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain a 1–70（70 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R48 Bovine complex I in the presence of IM1761092, deactive class iv (Composite map) Deposited 2022-02-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain a 1–70（70 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4C Bovine complex I in the presence of IM1761092, deactive class v (Composite map) Deposited 2022-02-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain a 1–70（70 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4D Bovine complex I in the presence of IM1761092, deactive class vi (Composite map) Deposited 2022-02-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain a 1–70（70 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4F Bovine complex I in the presence of IM1761092, slack class i (Composite map) Deposited 2022-02-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain a 1–70（70 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 7 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 CDL CARDIOLIPIN × 6 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R4G Bovine complex I in the presence of IM1761092, slack class ii (Composite map) Deposited 2022-02-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain a 1–70（70 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 10 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8Q0A Inward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain a 1–70（70 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 8 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0F Inward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain a 1–70（70 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 21 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0J Inward-facing, slack proteoliposome complex I at 3.8 A. Initially purified in DDM. Deposited 2023-07-28 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain a 1–70（70 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.80 Å
8Q0M Outward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain a 1–70（70 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0O Outward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain a 1–70（70 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0Q Outward-facing, slack proteoliposome complex I at 3.6 A. Initially purified in DDM Deposited 2023-07-28 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain a 1–70（70 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.60 Å
8Q1P Inward-facing, open2 proteoliposome complex I at 2.9 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain a 1–70（70 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q1U Inward-facing, open1 proteoliposome complex I at 3.3 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain a 1–70（70 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.30 Å
8Q1Y Outward-facing, open2 proteoliposome complex I at 2.6 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain a 1–70（70 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q25 Outward-facing, open1 proteoliposome complex I at 2.8 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain a 1–70（70 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.80 Å
8Q45 Inward-facing, closed proteoliposome complex I at 2.7 A. Initially purified in LMNG. Deposited 2023-08-05 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain a 1–70（70 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.70 Å
8Q46 Inward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain a 1–70（70 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q47 Inward-facing, open1 proteoliposome complex I at 2.9 A. Initially purified in LMNG. Deposited 2023-08-05 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain a 1–70（70 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 26 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q48 Outward-facing, closed proteoliposome complex I at 2.5 A. Initially purified in LMNG. Deposited 2023-08-05 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain a 1–70（70 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 35 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.50 Å
8Q49 Outward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain a 1–70（70 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 FVH [[(2~{R},3~{S},4~{R},5~{R})-5-[(3~{R})-3-aminocarbonylpiperidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2~{R},3~{R},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3-oxidanyl-4-phosphonooxy-oxolan-2-yl]methyl hydrogen phosphate × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q4A Outward-facing, open1 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain a 1–70（70 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
9SMF Reduced bovine complex I in lipid nanodisc, NADH-active-Q10 Deposited 2025-09-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain a 1–70（70 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.51 Å
9SMG Reduced bovine complex I in lipid nanodisc, NADH-active-altQ10 Deposited 2025-09-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain a 1–70（70 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.42 Å
9SMH Reduced bovine complex I in lipid nanodisc, NADH-active-DDM Deposited 2025-09-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain a 1–70（70 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.61 Å
9SMI Reduced bovine complex I in lipid nanodisc, NADH-deactive Deposited 2025-09-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain a 1–70（70 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 27 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 LMT DODECYL-BETA-D-MALTOSIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 CHD CHOLIC ACID × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.01 Å
9W2U Cryo-EM structure of complex I on the bovine heart submitochondrial particles, open Deposited 2025-07-28 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain a 1–70（70 aa）
Not recorded FME N-FORMYLMETHIONINE × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å

49 other PDB entries and 49 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUA1_BOVIN
Isoform —
PDB entities 27
Chains and sequence ranges Author chain a； PDBConstruct 1–70； UniProt 1–70

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3

Bos taurus

UniProt Q02371

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain b； UniProt 2–84 Not recorded NADH-ubiquinone oxidoreductase chain 3 × 1 （Q8WAB4） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （P42026） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （P23709） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （P17694） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P04394） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P25708） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P15690） NADH-ubiquinone oxidoreductase chain 1 × 1 （Q6EMT1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （P42028） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03924） NADH-ubiquinone oxidoreductase chain 4L × 1 （Q6EMS3） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03920） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03910） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03892） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （P34942） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （P34943） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q02375） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P23934） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q02370） Acyl carrier protein, mitochondrial × 1 （Q9CR21） Acyl carrier protein, mitochondrial × 1 （P52505） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （P23935） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q02366） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P42029） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q8HXG6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q95KV7） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （Q02377） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q02376） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q02827） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q02379） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （Q02378） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q8HXG5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q02380） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q02367） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q02374） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q02365） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q02372） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （P48305） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q02369） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q02368） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q02373） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （O97725） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q05752） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） FME N-FORMYLMETHIONINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 2.80 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9W2V Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5LC5 Structure of mammalian respiratory Complex I, class2 Deposited 2016-06-19 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain b 2–46（45 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 4.35 Å
5LDW Structure of mammalian respiratory Complex I, class1 Deposited 2016-06-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain b 2–46（45 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus present in a cold room, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 4.27 Å
5LDX Structure of mammalian respiratory Complex I, class3. Deposited 2016-06-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain b 2–46（45 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer 20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7；pH 8
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 5.60 Å
5O31 Mitochondrial complex I in the deactive state Deposited 2017-05-23 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain b 2–46（45 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.13 Å
7DGQ Activity optimized supercomplex state1 Deposited 2020-11-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain L 2–84（83 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 CU COPPER (II) ION × 3 HEA HEME-A × 2 MG MAGNESIUM ION × 1 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 UQ2 UBIQUINONE-2 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.00 Å
7DGR Activity optimized supercomplex state2 Deposited 2020-11-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain L 2–84（83 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 3 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.60 Å
7DGS Activity optimized supercomplex state3 Deposited 2020-11-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain L 2–84（83 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 7.80 Å
7DGZ Activity optimized complex I (closed form) Deposited 2020-11-12 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain L 2–84（83 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
7DH0 Activity optimized complex I (open form) Deposited 2020-11-12 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain L 2–84（83 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
7DKF Activity optimized supercomplex state4 Deposited 2020-11-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain L2 2–84（83 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 8.30 Å
7QSD Bovine complex I in the active state at 3.1 A Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain b 1–84（84 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 4 LMT DODECYL-BETA-D-MALTOSIDE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7QSK Bovine complex I in lipid nanodisc, Active-Q10 Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain b 1–84（84 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.84 Å
7QSL Bovine complex I in lipid nanodisc, Active-apo Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain b 1–84（84 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.76 Å
7QSM Bovine complex I in lipid nanodisc, Deactive-ligand (composite) Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain b 1–84（84 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 GOL GLYCEROL × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.30 Å
7QSN Bovine complex I in lipid nanodisc, Deactive-apo Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain b 1–84（84 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.81 Å
7QSO Bovine complex I in lipid nanodisc, State 3 (Slack) Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain b 1–84（84 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 6 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 2 CDL CARDIOLIPIN × 6 U10 UBIQUINONE-10 × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.02 Å
7R41 Bovine complex I in the presence of IM1761092, active class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain b 1–84（84 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R42 Bovine complex I in the presence of IM1761092, active class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain b 1–84（84 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R43 Bovine complex I in the presence of IM1761092, active class iii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain b 1–84（84 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 CDL CARDIOLIPIN × 5 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R44 Bovine complex I in the presence of IM1761092, active class iv (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain b 1–84（84 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R45 Bovine complex I in the presence of IM1761092, deactive class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain b 1–84（84 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R46 Bovine complex I in the presence of IM1761092, deactive class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain b 1–84（84 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 11 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R47 Bovine complex I in the presence of IM1761092, deactive class iii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain b 1–84（84 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R48 Bovine complex I in the presence of IM1761092, deactive class iv (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain b 1–84（84 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4C Bovine complex I in the presence of IM1761092, deactive class v (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain b 1–84（84 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4D Bovine complex I in the presence of IM1761092, deactive class vi (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain b 1–84（84 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4F Bovine complex I in the presence of IM1761092, slack class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain b 1–84（84 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 7 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 CDL CARDIOLIPIN × 6 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R4G Bovine complex I in the presence of IM1761092, slack class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain b 1–84（84 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 10 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8Q0A Inward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain b 1–84（84 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 8 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0F Inward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain b 1–84（84 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 21 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0J Inward-facing, slack proteoliposome complex I at 3.8 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain b 1–84（84 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.80 Å
8Q0M Outward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain b 1–84（84 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0O Outward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain b 1–84（84 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0Q Outward-facing, slack proteoliposome complex I at 3.6 A. Initially purified in DDM Deposited 2023-07-28 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain b 1–84（84 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.60 Å
8Q1P Inward-facing, open2 proteoliposome complex I at 2.9 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain b 1–84（84 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q1U Inward-facing, open1 proteoliposome complex I at 3.3 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain b 1–84（84 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.30 Å
8Q1Y Outward-facing, open2 proteoliposome complex I at 2.6 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain b 1–84（84 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q25 Outward-facing, open1 proteoliposome complex I at 2.8 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain b 1–84（84 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.80 Å
8Q45 Inward-facing, closed proteoliposome complex I at 2.7 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain b 1–84（84 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.70 Å
8Q46 Inward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain b 1–84（84 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q47 Inward-facing, open1 proteoliposome complex I at 2.9 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain b 1–84（84 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 26 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q48 Outward-facing, closed proteoliposome complex I at 2.5 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain b 1–84（84 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 35 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.50 Å
8Q49 Outward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain b 1–84（84 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 FVH [[(2~{R},3~{S},4~{R},5~{R})-5-[(3~{R})-3-aminocarbonylpiperidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2~{R},3~{R},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3-oxidanyl-4-phosphonooxy-oxolan-2-yl]methyl hydrogen phosphate × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q4A Outward-facing, open1 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain b 1–84（84 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
9SMF Reduced bovine complex I in lipid nanodisc, NADH-active-Q10 Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain b 1–84（84 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.51 Å
9SMG Reduced bovine complex I in lipid nanodisc, NADH-active-altQ10 Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain b 1–84（84 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.42 Å
9SMH Reduced bovine complex I in lipid nanodisc, NADH-active-DDM Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain b 1–84（84 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.61 Å
9SMI Reduced bovine complex I in lipid nanodisc, NADH-deactive Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain b 1–84（84 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 27 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 LMT DODECYL-BETA-D-MALTOSIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 CHD CHOLIC ACID × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.01 Å
9W2U Cryo-EM structure of complex I on the bovine heart submitochondrial particles, open Deposited 2025-07-28 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain b 2–84（83 aa）
Not recorded FME N-FORMYLMETHIONINE × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å

49 other PDB entries and 49 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUA3_BOVIN
Isoform —
PDB entities 28
Chains and sequence ranges Author chain b； PDBConstruct 1–83； UniProt 2–84

NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial

Bos taurus

UniProt Q02376

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain c； UniProt 28–76 Not recorded NADH-ubiquinone oxidoreductase chain 3 × 1 （Q8WAB4） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （P42026） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （P23709） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （P17694） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P04394） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P25708） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P15690） NADH-ubiquinone oxidoreductase chain 1 × 1 （Q6EMT1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （P42028） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03924） NADH-ubiquinone oxidoreductase chain 4L × 1 （Q6EMS3） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03920） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03910） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03892） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （P34942） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （P34943） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q02375） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P23934） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q02370） Acyl carrier protein, mitochondrial × 1 （Q9CR21） Acyl carrier protein, mitochondrial × 1 （P52505） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （P23935） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q02366） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P42029） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q8HXG6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q95KV7） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （Q02377） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q02371） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q02827） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q02379） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （Q02378） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q8HXG5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q02380） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q02367） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q02374） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q02365） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q02372） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （P48305） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q02369） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q02368） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q02373） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （O97725） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q05752） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） FME N-FORMYLMETHIONINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 2.80 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9W2V Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5LC5 Structure of mammalian respiratory Complex I, class2 Deposited 2016-06-19 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain c 1–76（76 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 4.35 Å
5LDW Structure of mammalian respiratory Complex I, class1 Deposited 2016-06-28 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain c 28–76（49 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus present in a cold room, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 4.27 Å
5LDX Structure of mammalian respiratory Complex I, class3. Deposited 2016-06-28 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain c 28–76（49 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer 20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7；pH 8
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 5.60 Å
5O31 Mitochondrial complex I in the deactive state Deposited 2017-05-23 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain c 28–76（49 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.13 Å
7DGQ Activity optimized supercomplex state1 Deposited 2020-11-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain i 28–76（49 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 CU COPPER (II) ION × 3 HEA HEME-A × 2 MG MAGNESIUM ION × 1 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 UQ2 UBIQUINONE-2 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.00 Å
7DGR Activity optimized supercomplex state2 Deposited 2020-11-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain i 28–76（49 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 3 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.60 Å
7DGS Activity optimized supercomplex state3 Deposited 2020-11-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain i 28–76（49 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 7.80 Å
7DGZ Activity optimized complex I (closed form) Deposited 2020-11-12 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain i 28–76（49 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
7DH0 Activity optimized complex I (open form) Deposited 2020-11-12 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain i 28–76（49 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
7DKF Activity optimized supercomplex state4 Deposited 2020-11-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain i2 28–76（49 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 8.30 Å
7QSD Bovine complex I in the active state at 3.1 A Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain c 1–76（76 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 4 LMT DODECYL-BETA-D-MALTOSIDE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7QSK Bovine complex I in lipid nanodisc, Active-Q10 Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain c 1–76（76 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.84 Å
7QSL Bovine complex I in lipid nanodisc, Active-apo Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain c 1–76（76 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.76 Å
7QSM Bovine complex I in lipid nanodisc, Deactive-ligand (composite) Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain c 1–76（76 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 GOL GLYCEROL × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.30 Å
7QSN Bovine complex I in lipid nanodisc, Deactive-apo Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain c 1–76（76 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.81 Å
7QSO Bovine complex I in lipid nanodisc, State 3 (Slack) Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain c 1–76（76 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 6 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 2 CDL CARDIOLIPIN × 6 U10 UBIQUINONE-10 × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.02 Å
7R41 Bovine complex I in the presence of IM1761092, active class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain c 1–76（76 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R42 Bovine complex I in the presence of IM1761092, active class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain c 1–76（76 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R43 Bovine complex I in the presence of IM1761092, active class iii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain c 1–76（76 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 CDL CARDIOLIPIN × 5 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R44 Bovine complex I in the presence of IM1761092, active class iv (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain c 1–76（76 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R45 Bovine complex I in the presence of IM1761092, deactive class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain c 1–76（76 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R46 Bovine complex I in the presence of IM1761092, deactive class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain c 1–76（76 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 11 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R47 Bovine complex I in the presence of IM1761092, deactive class iii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain c 1–76（76 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R48 Bovine complex I in the presence of IM1761092, deactive class iv (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain c 1–76（76 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4C Bovine complex I in the presence of IM1761092, deactive class v (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain c 1–76（76 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4D Bovine complex I in the presence of IM1761092, deactive class vi (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain c 1–76（76 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4F Bovine complex I in the presence of IM1761092, slack class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain c 1–76（76 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 7 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 CDL CARDIOLIPIN × 6 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R4G Bovine complex I in the presence of IM1761092, slack class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain c 1–76（76 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 10 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8Q0A Inward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain c 1–76（76 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 8 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0F Inward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain c 1–76（76 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 21 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0J Inward-facing, slack proteoliposome complex I at 3.8 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain c 1–76（76 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.80 Å
8Q0M Outward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain c 1–76（76 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0O Outward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain c 1–76（76 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0Q Outward-facing, slack proteoliposome complex I at 3.6 A. Initially purified in DDM Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain c 1–76（76 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.60 Å
8Q1P Inward-facing, open2 proteoliposome complex I at 2.9 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain c 1–76（76 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q1U Inward-facing, open1 proteoliposome complex I at 3.3 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain c 1–76（76 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.30 Å
8Q1Y Outward-facing, open2 proteoliposome complex I at 2.6 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain c 1–76（76 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q25 Outward-facing, open1 proteoliposome complex I at 2.8 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain c 1–76（76 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.80 Å
8Q45 Inward-facing, closed proteoliposome complex I at 2.7 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain c 1–76（76 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.70 Å
8Q46 Inward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain c 1–76（76 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q47 Inward-facing, open1 proteoliposome complex I at 2.9 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain c 1–76（76 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 26 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q48 Outward-facing, closed proteoliposome complex I at 2.5 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain c 1–76（76 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 35 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.50 Å
8Q49 Outward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain c 1–76（76 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 FVH [[(2~{R},3~{S},4~{R},5~{R})-5-[(3~{R})-3-aminocarbonylpiperidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2~{R},3~{R},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3-oxidanyl-4-phosphonooxy-oxolan-2-yl]methyl hydrogen phosphate × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q4A Outward-facing, open1 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain c 1–76（76 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
9SMF Reduced bovine complex I in lipid nanodisc, NADH-active-Q10 Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain c 1–76（76 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.51 Å
9SMG Reduced bovine complex I in lipid nanodisc, NADH-active-altQ10 Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain c 1–76（76 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.42 Å
9SMH Reduced bovine complex I in lipid nanodisc, NADH-active-DDM Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain c 1–76（76 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.61 Å
9SMI Reduced bovine complex I in lipid nanodisc, NADH-deactive Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain c 1–76（76 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 27 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 LMT DODECYL-BETA-D-MALTOSIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 CHD CHOLIC ACID × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.01 Å
9W2U Cryo-EM structure of complex I on the bovine heart submitochondrial particles, open Deposited 2025-07-28 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain c 28–76（49 aa）
Not recorded FME N-FORMYLMETHIONINE × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å

49 other PDB entries and 49 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUC1_BOVIN
Isoform —
PDB entities 29
Chains and sequence ranges Author chain c； PDBConstruct 1–49； UniProt 28–76

NADH dehydrogenase [ubiquinone] 1 subunit C2

Bos taurus

UniProt Q02827

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain d； UniProt 2–120 Not recorded NADH-ubiquinone oxidoreductase chain 3 × 1 （Q8WAB4） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （P42026） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （P23709） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （P17694） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P04394） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P25708） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P15690） NADH-ubiquinone oxidoreductase chain 1 × 1 （Q6EMT1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （P42028） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03924） NADH-ubiquinone oxidoreductase chain 4L × 1 （Q6EMS3） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03920） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03910） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03892） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （P34942） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （P34943） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q02375） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P23934） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q02370） Acyl carrier protein, mitochondrial × 1 （Q9CR21） Acyl carrier protein, mitochondrial × 1 （P52505） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （P23935） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q02366） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P42029） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q8HXG6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q95KV7） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （Q02377） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q02371） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q02376） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q02379） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （Q02378） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q8HXG5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q02380） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q02367） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q02374） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q02365） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q02372） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （P48305） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q02369） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q02368） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q02373） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （O97725） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q05752） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） FME N-FORMYLMETHIONINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 2.80 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9W2V Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5LC5 Structure of mammalian respiratory Complex I, class2 Deposited 2016-06-19 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain d 29–97（69 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 4.35 Å
5LDW Structure of mammalian respiratory Complex I, class1 Deposited 2016-06-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain d 29–96（68 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus present in a cold room, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 4.27 Å
5LDX Structure of mammalian respiratory Complex I, class3. Deposited 2016-06-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain d 29–97（69 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer 20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7；pH 8
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 5.60 Å
5O31 Mitochondrial complex I in the deactive state Deposited 2017-05-23 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain d 1–97（97 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.13 Å
7DGQ Activity optimized supercomplex state1 Deposited 2020-11-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain j 1–120（120 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 CU COPPER (II) ION × 3 HEA HEME-A × 2 MG MAGNESIUM ION × 1 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 UQ2 UBIQUINONE-2 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.00 Å
7DGR Activity optimized supercomplex state2 Deposited 2020-11-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain j 1–120（120 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 3 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.60 Å
7DGS Activity optimized supercomplex state3 Deposited 2020-11-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain j 1–120（120 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 7.80 Å
7DGZ Activity optimized complex I (closed form) Deposited 2020-11-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain j 1–120（120 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
7DH0 Activity optimized complex I (open form) Deposited 2020-11-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain j 1–120（120 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
7DKF Activity optimized supercomplex state4 Deposited 2020-11-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain j2 1–120（120 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 8.30 Å
7QSD Bovine complex I in the active state at 3.1 A Deposited 2022-01-13 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain d 1–120（120 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 4 LMT DODECYL-BETA-D-MALTOSIDE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7QSK Bovine complex I in lipid nanodisc, Active-Q10 Deposited 2022-01-13 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain d 1–120（120 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.84 Å
7QSL Bovine complex I in lipid nanodisc, Active-apo Deposited 2022-01-13 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain d 1–120（120 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.76 Å
7QSM Bovine complex I in lipid nanodisc, Deactive-ligand (composite) Deposited 2022-01-13 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain d 1–120（120 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 GOL GLYCEROL × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.30 Å
7QSN Bovine complex I in lipid nanodisc, Deactive-apo Deposited 2022-01-13 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain d 1–120（120 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.81 Å
7QSO Bovine complex I in lipid nanodisc, State 3 (Slack) Deposited 2022-01-13 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain d 1–120（120 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 6 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 2 CDL CARDIOLIPIN × 6 U10 UBIQUINONE-10 × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.02 Å
7R41 Bovine complex I in the presence of IM1761092, active class i (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain d 1–120（120 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R42 Bovine complex I in the presence of IM1761092, active class ii (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain d 1–120（120 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R43 Bovine complex I in the presence of IM1761092, active class iii (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain d 1–120（120 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 CDL CARDIOLIPIN × 5 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R44 Bovine complex I in the presence of IM1761092, active class iv (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain d 1–120（120 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R45 Bovine complex I in the presence of IM1761092, deactive class i (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain d 1–120（120 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R46 Bovine complex I in the presence of IM1761092, deactive class ii (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain d 1–120（120 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 11 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R47 Bovine complex I in the presence of IM1761092, deactive class iii (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain d 1–120（120 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R48 Bovine complex I in the presence of IM1761092, deactive class iv (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain d 1–120（120 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4C Bovine complex I in the presence of IM1761092, deactive class v (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain d 1–120（120 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4D Bovine complex I in the presence of IM1761092, deactive class vi (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain d 1–120（120 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4F Bovine complex I in the presence of IM1761092, slack class i (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain d 1–120（120 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 7 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 CDL CARDIOLIPIN × 6 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R4G Bovine complex I in the presence of IM1761092, slack class ii (Composite map) Deposited 2022-02-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain d 1–120（120 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 10 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8Q0A Inward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain d 1–120（120 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 8 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0F Inward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain d 1–120（120 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 21 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0J Inward-facing, slack proteoliposome complex I at 3.8 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain d 1–120（120 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.80 Å
8Q0M Outward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain d 1–120（120 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0O Outward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain d 1–120（120 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0Q Outward-facing, slack proteoliposome complex I at 3.6 A. Initially purified in DDM Deposited 2023-07-28 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain d 1–120（120 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.60 Å
8Q1P Inward-facing, open2 proteoliposome complex I at 2.9 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain d 1–120（120 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q1U Inward-facing, open1 proteoliposome complex I at 3.3 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain d 1–120（120 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.30 Å
8Q1Y Outward-facing, open2 proteoliposome complex I at 2.6 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain d 1–120（120 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q25 Outward-facing, open1 proteoliposome complex I at 2.8 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain d 1–120（120 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.80 Å
8Q45 Inward-facing, closed proteoliposome complex I at 2.7 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain d 1–120（120 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.70 Å
8Q46 Inward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain d 1–120（120 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q47 Inward-facing, open1 proteoliposome complex I at 2.9 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain d 1–120（120 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 26 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q48 Outward-facing, closed proteoliposome complex I at 2.5 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain d 1–120（120 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 35 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.50 Å
8Q49 Outward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain d 1–120（120 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 FVH [[(2~{R},3~{S},4~{R},5~{R})-5-[(3~{R})-3-aminocarbonylpiperidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2~{R},3~{R},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3-oxidanyl-4-phosphonooxy-oxolan-2-yl]methyl hydrogen phosphate × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q4A Outward-facing, open1 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain d 1–120（120 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
9SMF Reduced bovine complex I in lipid nanodisc, NADH-active-Q10 Deposited 2025-09-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain d 1–120（120 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.51 Å
9SMG Reduced bovine complex I in lipid nanodisc, NADH-active-altQ10 Deposited 2025-09-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain d 1–120（120 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.42 Å
9SMH Reduced bovine complex I in lipid nanodisc, NADH-active-DDM Deposited 2025-09-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain d 1–120（120 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.61 Å
9SMI Reduced bovine complex I in lipid nanodisc, NADH-deactive Deposited 2025-09-08 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain d 1–120（120 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 27 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 LMT DODECYL-BETA-D-MALTOSIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 CHD CHOLIC ACID × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.01 Å
9W2U Cryo-EM structure of complex I on the bovine heart submitochondrial particles, open Deposited 2025-07-28 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain d 2–120（119 aa）
Not recorded FME N-FORMYLMETHIONINE × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å

49 other PDB entries and 49 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUC2_BOVIN
Isoform —
PDB entities 30
Chains and sequence ranges Author chain d； PDBConstruct 1–119； UniProt 2–120

NADH dehydrogenase [ubiquinone] iron-sulfur protein 5

Bos taurus

UniProt Q02379

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain e； UniProt 2–100 Not recorded NADH-ubiquinone oxidoreductase chain 3 × 1 （Q8WAB4） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （P42026） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （P23709） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （P17694） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P04394） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P25708） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P15690） NADH-ubiquinone oxidoreductase chain 1 × 1 （Q6EMT1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （P42028） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03924） NADH-ubiquinone oxidoreductase chain 4L × 1 （Q6EMS3） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03920） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03910） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03892） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （P34942） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （P34943） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q02375） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P23934） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q02370） Acyl carrier protein, mitochondrial × 1 （Q9CR21） Acyl carrier protein, mitochondrial × 1 （P52505） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （P23935） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q02366） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P42029） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q8HXG6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q95KV7） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （Q02377） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q02371） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q02376） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q02827） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （Q02378） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q8HXG5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q02380） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q02367） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q02374） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q02365） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q02372） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （P48305） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q02369） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q02368） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q02373） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （O97725） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q05752） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） FME N-FORMYLMETHIONINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 2.80 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9W2V Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5LC5 Structure of mammalian respiratory Complex I, class2 Deposited 2016-06-19 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain e 1–106（106 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 4.35 Å
5LDW Structure of mammalian respiratory Complex I, class1 Deposited 2016-06-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain e 2–106（105 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus present in a cold room, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 4.27 Å
5LDX Structure of mammalian respiratory Complex I, class3. Deposited 2016-06-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain e 1–106（106 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer 20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7；pH 8
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 5.60 Å
5O31 Mitochondrial complex I in the deactive state Deposited 2017-05-23 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain e 2–106（105 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.13 Å
7DGQ Activity optimized supercomplex state1 Deposited 2020-11-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain H 2–106（105 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 CU COPPER (II) ION × 3 HEA HEME-A × 2 MG MAGNESIUM ION × 1 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 UQ2 UBIQUINONE-2 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.00 Å
7DGR Activity optimized supercomplex state2 Deposited 2020-11-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain H 2–106（105 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 3 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.60 Å
7DGS Activity optimized supercomplex state3 Deposited 2020-11-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain H 2–106（105 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 7.80 Å
7DGZ Activity optimized complex I (closed form) Deposited 2020-11-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain H 2–106（105 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
7DH0 Activity optimized complex I (open form) Deposited 2020-11-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain H 2–106（105 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
7DKF Activity optimized supercomplex state4 Deposited 2020-11-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain H2 2–106（105 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 8.30 Å
7QSD Bovine complex I in the active state at 3.1 A Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain e 1–106（106 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 4 LMT DODECYL-BETA-D-MALTOSIDE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7QSK Bovine complex I in lipid nanodisc, Active-Q10 Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain e 1–106（106 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.84 Å
7QSL Bovine complex I in lipid nanodisc, Active-apo Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain e 1–106（106 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.76 Å
7QSM Bovine complex I in lipid nanodisc, Deactive-ligand (composite) Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain e 1–106（106 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 GOL GLYCEROL × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.30 Å
7QSN Bovine complex I in lipid nanodisc, Deactive-apo Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain e 1–106（106 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.81 Å
7QSO Bovine complex I in lipid nanodisc, State 3 (Slack) Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain e 1–106（106 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 6 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 2 CDL CARDIOLIPIN × 6 U10 UBIQUINONE-10 × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.02 Å
7R41 Bovine complex I in the presence of IM1761092, active class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain e 1–106（106 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R42 Bovine complex I in the presence of IM1761092, active class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain e 1–106（106 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R43 Bovine complex I in the presence of IM1761092, active class iii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain e 1–106（106 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 CDL CARDIOLIPIN × 5 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R44 Bovine complex I in the presence of IM1761092, active class iv (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain e 1–106（106 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R45 Bovine complex I in the presence of IM1761092, deactive class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain e 1–106（106 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R46 Bovine complex I in the presence of IM1761092, deactive class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain e 1–106（106 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 11 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R47 Bovine complex I in the presence of IM1761092, deactive class iii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain e 1–106（106 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R48 Bovine complex I in the presence of IM1761092, deactive class iv (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain e 1–106（106 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4C Bovine complex I in the presence of IM1761092, deactive class v (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain e 1–106（106 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4D Bovine complex I in the presence of IM1761092, deactive class vi (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain e 1–106（106 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4F Bovine complex I in the presence of IM1761092, slack class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain e 1–106（106 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 7 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 CDL CARDIOLIPIN × 6 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R4G Bovine complex I in the presence of IM1761092, slack class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain e 1–106（106 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 10 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8Q0A Inward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain e 1–106（106 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 8 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0F Inward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain e 1–106（106 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 21 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0J Inward-facing, slack proteoliposome complex I at 3.8 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain e 1–106（106 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.80 Å
8Q0M Outward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain e 1–106（106 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0O Outward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain e 1–106（106 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0Q Outward-facing, slack proteoliposome complex I at 3.6 A. Initially purified in DDM Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain e 1–106（106 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.60 Å
8Q1P Inward-facing, open2 proteoliposome complex I at 2.9 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain e 1–106（106 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q1U Inward-facing, open1 proteoliposome complex I at 3.3 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain e 1–106（106 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.30 Å
8Q1Y Outward-facing, open2 proteoliposome complex I at 2.6 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain e 1–106（106 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q25 Outward-facing, open1 proteoliposome complex I at 2.8 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain e 1–106（106 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.80 Å
8Q45 Inward-facing, closed proteoliposome complex I at 2.7 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain e 1–106（106 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.70 Å
8Q46 Inward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain e 1–106（106 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q47 Inward-facing, open1 proteoliposome complex I at 2.9 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain e 1–106（106 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 26 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q48 Outward-facing, closed proteoliposome complex I at 2.5 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain e 1–106（106 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 35 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.50 Å
8Q49 Outward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain e 1–106（106 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 FVH [[(2~{R},3~{S},4~{R},5~{R})-5-[(3~{R})-3-aminocarbonylpiperidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2~{R},3~{R},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3-oxidanyl-4-phosphonooxy-oxolan-2-yl]methyl hydrogen phosphate × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q4A Outward-facing, open1 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain e 1–106（106 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
9SMF Reduced bovine complex I in lipid nanodisc, NADH-active-Q10 Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain e 1–106（106 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.51 Å
9SMG Reduced bovine complex I in lipid nanodisc, NADH-active-altQ10 Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain e 1–106（106 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.42 Å
9SMH Reduced bovine complex I in lipid nanodisc, NADH-active-DDM Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain e 1–106（106 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.61 Å
9SMI Reduced bovine complex I in lipid nanodisc, NADH-deactive Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain e 1–106（106 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 27 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 LMT DODECYL-BETA-D-MALTOSIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 CHD CHOLIC ACID × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.01 Å
9W2U Cryo-EM structure of complex I on the bovine heart submitochondrial particles, open Deposited 2025-07-28 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain e 2–100（99 aa）
Not recorded FME N-FORMYLMETHIONINE × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å

49 other PDB entries and 49 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUS5_BOVIN
Isoform —
PDB entities 31
Chains and sequence ranges Author chain e； PDBConstruct 1–99； UniProt 2–100

NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1

Bos taurus

UniProt Q02378

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain f； UniProt 1–57 Not recorded NADH-ubiquinone oxidoreductase chain 3 × 1 （Q8WAB4） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （P42026） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （P23709） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （P17694） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P04394） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P25708） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P15690） NADH-ubiquinone oxidoreductase chain 1 × 1 （Q6EMT1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （P42028） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03924） NADH-ubiquinone oxidoreductase chain 4L × 1 （Q6EMS3） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03920） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03910） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03892） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （P34942） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （P34943） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q02375） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P23934） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q02370） Acyl carrier protein, mitochondrial × 1 （Q9CR21） Acyl carrier protein, mitochondrial × 1 （P52505） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （P23935） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q02366） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P42029） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q8HXG6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q95KV7） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （Q02377） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q02371） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q02376） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q02827） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q02379） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q8HXG5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q02380） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q02367） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q02374） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q02365） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q02372） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （P48305） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q02369） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q02368） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q02373） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （O97725） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q05752） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） FME N-FORMYLMETHIONINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 2.80 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9W2V Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5LC5 Structure of mammalian respiratory Complex I, class2 Deposited 2016-06-19 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain f 1–57（57 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 4.35 Å
5LDW Structure of mammalian respiratory Complex I, class1 Deposited 2016-06-28 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain f 1–57（57 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus present in a cold room, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 4.27 Å
5LDX Structure of mammalian respiratory Complex I, class3. Deposited 2016-06-28 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain f 1–57（57 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer 20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7；pH 8
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 5.60 Å
5O31 Mitochondrial complex I in the deactive state Deposited 2017-05-23 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain f 1–57（57 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.13 Å
7DGQ Activity optimized supercomplex state1 Deposited 2020-11-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain X 1–57（57 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 CU COPPER (II) ION × 3 HEA HEME-A × 2 MG MAGNESIUM ION × 1 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 UQ2 UBIQUINONE-2 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.00 Å
7DGR Activity optimized supercomplex state2 Deposited 2020-11-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain X 1–57（57 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 3 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.60 Å
7DGS Activity optimized supercomplex state3 Deposited 2020-11-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain X 1–57（57 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 7.80 Å
7DGZ Activity optimized complex I (closed form) Deposited 2020-11-12 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain X 1–57（57 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
7DH0 Activity optimized complex I (open form) Deposited 2020-11-12 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain X 1–57（57 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
7DKF Activity optimized supercomplex state4 Deposited 2020-11-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain X2 1–57（57 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 8.30 Å
7QSD Bovine complex I in the active state at 3.1 A Deposited 2022-01-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain f 1–57（57 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 4 LMT DODECYL-BETA-D-MALTOSIDE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7QSK Bovine complex I in lipid nanodisc, Active-Q10 Deposited 2022-01-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain f 1–57（57 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.84 Å
7QSL Bovine complex I in lipid nanodisc, Active-apo Deposited 2022-01-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain f 1–57（57 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.76 Å
7QSM Bovine complex I in lipid nanodisc, Deactive-ligand (composite) Deposited 2022-01-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain f 1–57（57 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 GOL GLYCEROL × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.30 Å
7QSN Bovine complex I in lipid nanodisc, Deactive-apo Deposited 2022-01-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain f 1–57（57 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.81 Å
7QSO Bovine complex I in lipid nanodisc, State 3 (Slack) Deposited 2022-01-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain f 1–57（57 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 6 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 2 CDL CARDIOLIPIN × 6 U10 UBIQUINONE-10 × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.02 Å
7R41 Bovine complex I in the presence of IM1761092, active class i (Composite map) Deposited 2022-02-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain f 1–57（57 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R42 Bovine complex I in the presence of IM1761092, active class ii (Composite map) Deposited 2022-02-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain f 1–57（57 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R43 Bovine complex I in the presence of IM1761092, active class iii (Composite map) Deposited 2022-02-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain f 1–57（57 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 CDL CARDIOLIPIN × 5 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R44 Bovine complex I in the presence of IM1761092, active class iv (Composite map) Deposited 2022-02-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain f 1–57（57 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R45 Bovine complex I in the presence of IM1761092, deactive class i (Composite map) Deposited 2022-02-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain f 1–57（57 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R46 Bovine complex I in the presence of IM1761092, deactive class ii (Composite map) Deposited 2022-02-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain f 1–57（57 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 11 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R47 Bovine complex I in the presence of IM1761092, deactive class iii (Composite map) Deposited 2022-02-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain f 1–57（57 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R48 Bovine complex I in the presence of IM1761092, deactive class iv (Composite map) Deposited 2022-02-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain f 1–57（57 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4C Bovine complex I in the presence of IM1761092, deactive class v (Composite map) Deposited 2022-02-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain f 1–57（57 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4D Bovine complex I in the presence of IM1761092, deactive class vi (Composite map) Deposited 2022-02-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain f 1–57（57 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4F Bovine complex I in the presence of IM1761092, slack class i (Composite map) Deposited 2022-02-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain f 1–57（57 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 7 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 CDL CARDIOLIPIN × 6 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R4G Bovine complex I in the presence of IM1761092, slack class ii (Composite map) Deposited 2022-02-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain f 1–57（57 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 10 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8Q0A Inward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain f 1–57（57 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 8 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0F Inward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain f 1–57（57 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 21 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0J Inward-facing, slack proteoliposome complex I at 3.8 A. Initially purified in DDM. Deposited 2023-07-28 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain f 1–57（57 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.80 Å
8Q0M Outward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain f 1–57（57 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0O Outward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain f 1–57（57 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0Q Outward-facing, slack proteoliposome complex I at 3.6 A. Initially purified in DDM Deposited 2023-07-28 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain f 1–57（57 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.60 Å
8Q1P Inward-facing, open2 proteoliposome complex I at 2.9 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain f 1–57（57 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q1U Inward-facing, open1 proteoliposome complex I at 3.3 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain f 1–57（57 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.30 Å
8Q1Y Outward-facing, open2 proteoliposome complex I at 2.6 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain f 1–57（57 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q25 Outward-facing, open1 proteoliposome complex I at 2.8 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain f 1–57（57 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.80 Å
8Q45 Inward-facing, closed proteoliposome complex I at 2.7 A. Initially purified in LMNG. Deposited 2023-08-05 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain f 1–57（57 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.70 Å
8Q46 Inward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain f 1–57（57 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q47 Inward-facing, open1 proteoliposome complex I at 2.9 A. Initially purified in LMNG. Deposited 2023-08-05 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain f 1–57（57 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 26 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q48 Outward-facing, closed proteoliposome complex I at 2.5 A. Initially purified in LMNG. Deposited 2023-08-05 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain f 1–57（57 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 35 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.50 Å
8Q49 Outward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain f 1–57（57 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 FVH [[(2~{R},3~{S},4~{R},5~{R})-5-[(3~{R})-3-aminocarbonylpiperidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2~{R},3~{R},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3-oxidanyl-4-phosphonooxy-oxolan-2-yl]methyl hydrogen phosphate × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q4A Outward-facing, open1 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain f 1–57（57 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
9SMF Reduced bovine complex I in lipid nanodisc, NADH-active-Q10 Deposited 2025-09-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain f 1–57（57 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.51 Å
9SMG Reduced bovine complex I in lipid nanodisc, NADH-active-altQ10 Deposited 2025-09-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain f 1–57（57 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.42 Å
9SMH Reduced bovine complex I in lipid nanodisc, NADH-active-DDM Deposited 2025-09-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain f 1–57（57 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.61 Å
9SMI Reduced bovine complex I in lipid nanodisc, NADH-deactive Deposited 2025-09-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain f 1–57（57 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 27 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 LMT DODECYL-BETA-D-MALTOSIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 CHD CHOLIC ACID × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.01 Å
9W2U Cryo-EM structure of complex I on the bovine heart submitochondrial particles, open Deposited 2025-07-28 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain f 1–57（57 aa）
Not recorded FME N-FORMYLMETHIONINE × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å

49 other PDB entries and 49 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUB1_BOVIN
Isoform —
PDB entities 32
Chains and sequence ranges Author chain f； PDBConstruct 1–57； UniProt 1–57

NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial

Bos taurus

UniProt Q8HXG5

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain g； UniProt 52–151 Not recorded NADH-ubiquinone oxidoreductase chain 3 × 1 （Q8WAB4） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （P42026） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （P23709） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （P17694） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P04394） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P25708） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P15690） NADH-ubiquinone oxidoreductase chain 1 × 1 （Q6EMT1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （P42028） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03924） NADH-ubiquinone oxidoreductase chain 4L × 1 （Q6EMS3） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03920） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03910） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03892） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （P34942） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （P34943） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q02375） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P23934） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q02370） Acyl carrier protein, mitochondrial × 1 （Q9CR21） Acyl carrier protein, mitochondrial × 1 （P52505） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （P23935） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q02366） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P42029） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q8HXG6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q95KV7） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （Q02377） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q02371） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q02376） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q02827） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q02379） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （Q02378） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q02380） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q02367） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q02374） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q02365） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q02372） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （P48305） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q02369） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q02368） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q02373） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （O97725） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q05752） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） FME N-FORMYLMETHIONINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 2.80 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9W2V Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5LC5 Structure of mammalian respiratory Complex I, class2 Deposited 2016-06-19 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain g 1–154（154 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 4.35 Å
5LDW Structure of mammalian respiratory Complex I, class1 Deposited 2016-06-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain g 30–154（125 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus present in a cold room, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 4.27 Å
5LDX Structure of mammalian respiratory Complex I, class3. Deposited 2016-06-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain g 30–154（125 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer 20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7；pH 8
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 5.60 Å
5O31 Mitochondrial complex I in the deactive state Deposited 2017-05-23 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain g 30–154（125 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.13 Å
7DGQ Activity optimized supercomplex state1 Deposited 2020-11-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain h 30–154（125 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 CU COPPER (II) ION × 3 HEA HEME-A × 2 MG MAGNESIUM ION × 1 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 UQ2 UBIQUINONE-2 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.00 Å
7DGR Activity optimized supercomplex state2 Deposited 2020-11-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain h 30–154（125 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 3 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.60 Å
7DGS Activity optimized supercomplex state3 Deposited 2020-11-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain h 30–154（125 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 7.80 Å
7DGZ Activity optimized complex I (closed form) Deposited 2020-11-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain h 30–154（125 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
7DH0 Activity optimized complex I (open form) Deposited 2020-11-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain h 30–154（125 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
7DKF Activity optimized supercomplex state4 Deposited 2020-11-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain h2 30–154（125 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 8.30 Å
7QSD Bovine complex I in the active state at 3.1 A Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain g 1–154（154 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 4 LMT DODECYL-BETA-D-MALTOSIDE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7QSK Bovine complex I in lipid nanodisc, Active-Q10 Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain g 1–154（154 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.84 Å
7QSL Bovine complex I in lipid nanodisc, Active-apo Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain g 1–154（154 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.76 Å
7QSM Bovine complex I in lipid nanodisc, Deactive-ligand (composite) Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain g 1–154（154 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 GOL GLYCEROL × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.30 Å
7QSN Bovine complex I in lipid nanodisc, Deactive-apo Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain g 1–154（154 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.81 Å
7QSO Bovine complex I in lipid nanodisc, State 3 (Slack) Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain g 1–154（154 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 6 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 2 CDL CARDIOLIPIN × 6 U10 UBIQUINONE-10 × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.02 Å
7R41 Bovine complex I in the presence of IM1761092, active class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain g 1–154（154 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R42 Bovine complex I in the presence of IM1761092, active class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain g 1–154（154 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R43 Bovine complex I in the presence of IM1761092, active class iii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain g 1–154（154 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 CDL CARDIOLIPIN × 5 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R44 Bovine complex I in the presence of IM1761092, active class iv (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain g 1–154（154 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R45 Bovine complex I in the presence of IM1761092, deactive class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain g 1–154（154 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R46 Bovine complex I in the presence of IM1761092, deactive class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain g 1–154（154 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 11 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R47 Bovine complex I in the presence of IM1761092, deactive class iii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain g 1–154（154 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R48 Bovine complex I in the presence of IM1761092, deactive class iv (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain g 1–154（154 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4C Bovine complex I in the presence of IM1761092, deactive class v (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain g 1–154（154 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4D Bovine complex I in the presence of IM1761092, deactive class vi (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain g 1–154（154 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4F Bovine complex I in the presence of IM1761092, slack class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain g 1–154（154 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 7 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 CDL CARDIOLIPIN × 6 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R4G Bovine complex I in the presence of IM1761092, slack class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain g 1–154（154 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 10 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8Q0A Inward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain g 1–154（154 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 8 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0F Inward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain g 1–154（154 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 21 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0J Inward-facing, slack proteoliposome complex I at 3.8 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain g 1–154（154 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.80 Å
8Q0M Outward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain g 1–154（154 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0O Outward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain g 1–154（154 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0Q Outward-facing, slack proteoliposome complex I at 3.6 A. Initially purified in DDM Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain g 1–154（154 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.60 Å
8Q1P Inward-facing, open2 proteoliposome complex I at 2.9 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain g 1–154（154 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q1U Inward-facing, open1 proteoliposome complex I at 3.3 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain g 1–154（154 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.30 Å
8Q1Y Outward-facing, open2 proteoliposome complex I at 2.6 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain g 1–154（154 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q25 Outward-facing, open1 proteoliposome complex I at 2.8 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain g 1–154（154 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.80 Å
8Q45 Inward-facing, closed proteoliposome complex I at 2.7 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain g 1–154（154 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.70 Å
8Q46 Inward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain g 1–154（154 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q47 Inward-facing, open1 proteoliposome complex I at 2.9 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain g 1–154（154 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 26 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q48 Outward-facing, closed proteoliposome complex I at 2.5 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain g 1–154（154 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 35 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.50 Å
8Q49 Outward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain g 1–154（154 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 FVH [[(2~{R},3~{S},4~{R},5~{R})-5-[(3~{R})-3-aminocarbonylpiperidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2~{R},3~{R},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3-oxidanyl-4-phosphonooxy-oxolan-2-yl]methyl hydrogen phosphate × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q4A Outward-facing, open1 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain g 1–154（154 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
9SMF Reduced bovine complex I in lipid nanodisc, NADH-active-Q10 Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain g 1–154（154 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.51 Å
9SMG Reduced bovine complex I in lipid nanodisc, NADH-active-altQ10 Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain g 1–154（154 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.42 Å
9SMH Reduced bovine complex I in lipid nanodisc, NADH-active-DDM Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain g 1–154（154 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.61 Å
9SMI Reduced bovine complex I in lipid nanodisc, NADH-deactive Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain g 1–154（154 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 27 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 LMT DODECYL-BETA-D-MALTOSIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 CHD CHOLIC ACID × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.01 Å
9W2U Cryo-EM structure of complex I on the bovine heart submitochondrial particles, open Deposited 2025-07-28 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain g 52–151（100 aa）
Not recorded FME N-FORMYLMETHIONINE × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å

49 other PDB entries and 49 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUBB_BOVIN
Isoform —
PDB entities 33
Chains and sequence ranges Author chain g； PDBConstruct 1–100； UniProt 52–151

NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial

Bos taurus

UniProt Q02380

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain h； UniProt 52–189 Not recorded NADH-ubiquinone oxidoreductase chain 3 × 1 （Q8WAB4） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （P42026） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （P23709） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （P17694） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P04394） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P25708） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P15690） NADH-ubiquinone oxidoreductase chain 1 × 1 （Q6EMT1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （P42028） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03924） NADH-ubiquinone oxidoreductase chain 4L × 1 （Q6EMS3） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03920） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03910） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03892） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （P34942） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （P34943） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q02375） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P23934） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q02370） Acyl carrier protein, mitochondrial × 1 （Q9CR21） Acyl carrier protein, mitochondrial × 1 （P52505） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （P23935） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q02366） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P42029） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q8HXG6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q95KV7） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （Q02377） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q02371） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q02376） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q02827） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q02379） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （Q02378） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q8HXG5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q02367） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q02374） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q02365） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q02372） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （P48305） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q02369） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q02368） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q02373） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （O97725） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q05752） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） FME N-FORMYLMETHIONINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 2.80 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9W2V Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5LC5 Structure of mammalian respiratory Complex I, class2 Deposited 2016-06-19 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain h 1–91（91 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 4.35 Å
5LDW Structure of mammalian respiratory Complex I, class1 Deposited 2016-06-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain h 53–91（39 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus present in a cold room, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 4.27 Å
5LDX Structure of mammalian respiratory Complex I, class3. Deposited 2016-06-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain h 53–91（39 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer 20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7；pH 8
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 5.60 Å
5O31 Mitochondrial complex I in the deactive state Deposited 2017-05-23 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain h 47–189（143 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.13 Å
7DGQ Activity optimized supercomplex state1 Deposited 2020-11-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain b 47–189（143 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 CU COPPER (II) ION × 3 HEA HEME-A × 2 MG MAGNESIUM ION × 1 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 UQ2 UBIQUINONE-2 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.00 Å
7DGR Activity optimized supercomplex state2 Deposited 2020-11-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain b 47–189（143 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 3 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.60 Å
7DGS Activity optimized supercomplex state3 Deposited 2020-11-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain b 47–189（143 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 7.80 Å
7DGZ Activity optimized complex I (closed form) Deposited 2020-11-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain b 47–189（143 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
7DH0 Activity optimized complex I (open form) Deposited 2020-11-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain b 47–189（143 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
7DKF Activity optimized supercomplex state4 Deposited 2020-11-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain b2 47–189（143 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 8.30 Å
7QSD Bovine complex I in the active state at 3.1 A Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain h 1–189（189 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 4 LMT DODECYL-BETA-D-MALTOSIDE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7QSK Bovine complex I in lipid nanodisc, Active-Q10 Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain h 1–189（189 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.84 Å
7QSL Bovine complex I in lipid nanodisc, Active-apo Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain h 1–189（189 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.76 Å
7QSM Bovine complex I in lipid nanodisc, Deactive-ligand (composite) Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain h 1–189（189 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 GOL GLYCEROL × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.30 Å
7QSN Bovine complex I in lipid nanodisc, Deactive-apo Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain h 1–189（189 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.81 Å
7QSO Bovine complex I in lipid nanodisc, State 3 (Slack) Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain h 1–189（189 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 6 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 2 CDL CARDIOLIPIN × 6 U10 UBIQUINONE-10 × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.02 Å
7R41 Bovine complex I in the presence of IM1761092, active class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain h 1–189（189 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R42 Bovine complex I in the presence of IM1761092, active class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain h 1–189（189 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R43 Bovine complex I in the presence of IM1761092, active class iii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain h 1–189（189 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 CDL CARDIOLIPIN × 5 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R44 Bovine complex I in the presence of IM1761092, active class iv (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain h 1–189（189 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R45 Bovine complex I in the presence of IM1761092, deactive class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain h 1–189（189 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R46 Bovine complex I in the presence of IM1761092, deactive class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain h 1–189（189 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 11 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R47 Bovine complex I in the presence of IM1761092, deactive class iii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain h 1–189（189 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R48 Bovine complex I in the presence of IM1761092, deactive class iv (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain h 1–189（189 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4C Bovine complex I in the presence of IM1761092, deactive class v (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain h 1–189（189 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4D Bovine complex I in the presence of IM1761092, deactive class vi (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain h 1–189（189 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4F Bovine complex I in the presence of IM1761092, slack class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain h 1–189（189 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 7 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 CDL CARDIOLIPIN × 6 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R4G Bovine complex I in the presence of IM1761092, slack class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain h 1–189（189 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 10 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8Q0A Inward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain h 1–189（189 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 8 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0F Inward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain h 1–189（189 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 21 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0J Inward-facing, slack proteoliposome complex I at 3.8 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain h 1–189（189 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.80 Å
8Q0M Outward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain h 1–189（189 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0O Outward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain h 1–189（189 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0Q Outward-facing, slack proteoliposome complex I at 3.6 A. Initially purified in DDM Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain h 1–189（189 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.60 Å
8Q1P Inward-facing, open2 proteoliposome complex I at 2.9 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain h 1–189（189 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q1U Inward-facing, open1 proteoliposome complex I at 3.3 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain h 1–189（189 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.30 Å
8Q1Y Outward-facing, open2 proteoliposome complex I at 2.6 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain h 1–189（189 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q25 Outward-facing, open1 proteoliposome complex I at 2.8 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain h 1–189（189 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.80 Å
8Q45 Inward-facing, closed proteoliposome complex I at 2.7 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain h 1–189（189 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.70 Å
8Q46 Inward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain h 1–189（189 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q47 Inward-facing, open1 proteoliposome complex I at 2.9 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain h 1–189（189 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 26 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q48 Outward-facing, closed proteoliposome complex I at 2.5 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain h 1–189（189 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 35 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.50 Å
8Q49 Outward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain h 1–189（189 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 FVH [[(2~{R},3~{S},4~{R},5~{R})-5-[(3~{R})-3-aminocarbonylpiperidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2~{R},3~{R},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3-oxidanyl-4-phosphonooxy-oxolan-2-yl]methyl hydrogen phosphate × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q4A Outward-facing, open1 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain h 1–189（189 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
9SMF Reduced bovine complex I in lipid nanodisc, NADH-active-Q10 Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain h 1–189（189 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.51 Å
9SMG Reduced bovine complex I in lipid nanodisc, NADH-active-altQ10 Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain h 1–189（189 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.42 Å
9SMH Reduced bovine complex I in lipid nanodisc, NADH-active-DDM Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain h 1–189（189 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.61 Å
9SMI Reduced bovine complex I in lipid nanodisc, NADH-deactive Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain h 1–189（189 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 27 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 LMT DODECYL-BETA-D-MALTOSIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 CHD CHOLIC ACID × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.01 Å
9W2U Cryo-EM structure of complex I on the bovine heart submitochondrial particles, open Deposited 2025-07-28 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain h 52–189（138 aa）
Not recorded FME N-FORMYLMETHIONINE × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å

49 other PDB entries and 49 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUB5_BOVIN
Isoform —
PDB entities 34
Chains and sequence ranges Author chain h； PDBConstruct 1–138； UniProt 52–189

NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6

Bos taurus

UniProt Q02367

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain u； UniProt 2–128 Non-standard monomer：Yes (specific site not provided by mmCIF) NADH-ubiquinone oxidoreductase chain 3 × 1 （Q8WAB4） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （P42026） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （P23709） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （P17694） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P04394） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P25708） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P15690） NADH-ubiquinone oxidoreductase chain 1 × 1 （Q6EMT1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （P42028） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03924） NADH-ubiquinone oxidoreductase chain 4L × 1 （Q6EMS3） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03920） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03910） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03892） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （P34942） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （P34943） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q02375） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P23934） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q02370） Acyl carrier protein, mitochondrial × 1 （Q9CR21） Acyl carrier protein, mitochondrial × 1 （P52505） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （P23935） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q02366） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P42029） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q8HXG6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q95KV7） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （Q02377） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q02371） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q02376） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q02827） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q02379） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （Q02378） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q8HXG5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q02380） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q02374） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q02365） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q02372） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （P48305） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q02369） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q02368） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q02373） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （O97725） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q05752） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） FME N-FORMYLMETHIONINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 2.80 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9W2V Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5LC5 Structure of mammalian respiratory Complex I, class2 Deposited 2016-06-19 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain i 1–33（33 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 4.35 Å
5LDW Structure of mammalian respiratory Complex I, class1 Deposited 2016-06-28 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain i 7–33（27 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus present in a cold room, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 4.27 Å
5LDX Structure of mammalian respiratory Complex I, class3. Deposited 2016-06-28 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain i 7–33（27 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer 20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7；pH 8
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 5.60 Å
5O31 Mitochondrial complex I in the deactive state Deposited 2017-05-23 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain i 2–33（32 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.13 Å
7DGQ Activity optimized supercomplex state1 Deposited 2020-11-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain c 1–128（128 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 CU COPPER (II) ION × 3 HEA HEME-A × 2 MG MAGNESIUM ION × 1 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 UQ2 UBIQUINONE-2 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.00 Å
7DGR Activity optimized supercomplex state2 Deposited 2020-11-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain c 1–128（128 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 3 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.60 Å
7DGS Activity optimized supercomplex state3 Deposited 2020-11-12 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain c 2–128（127 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 7.80 Å
7DGZ Activity optimized complex I (closed form) Deposited 2020-11-12 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain c 2–128（127 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
7DH0 Activity optimized complex I (open form) Deposited 2020-11-12 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain c 2–128（127 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
7DKF Activity optimized supercomplex state4 Deposited 2020-11-24 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain c2 2–128（127 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 8.30 Å
7QSD Bovine complex I in the active state at 3.1 A Deposited 2022-01-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain i 2–128（127 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 4 LMT DODECYL-BETA-D-MALTOSIDE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7QSK Bovine complex I in lipid nanodisc, Active-Q10 Deposited 2022-01-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain i 2–128（127 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.84 Å
7QSL Bovine complex I in lipid nanodisc, Active-apo Deposited 2022-01-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain i 2–128（127 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.76 Å
7QSM Bovine complex I in lipid nanodisc, Deactive-ligand (composite) Deposited 2022-01-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain i 2–128（127 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 GOL GLYCEROL × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.30 Å
7QSN Bovine complex I in lipid nanodisc, Deactive-apo Deposited 2022-01-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain i 2–128（127 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.81 Å
7QSO Bovine complex I in lipid nanodisc, State 3 (Slack) Deposited 2022-01-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain i 2–128（127 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 6 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 2 CDL CARDIOLIPIN × 6 U10 UBIQUINONE-10 × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.02 Å
7R41 Bovine complex I in the presence of IM1761092, active class i (Composite map) Deposited 2022-02-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain i 2–128（127 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R42 Bovine complex I in the presence of IM1761092, active class ii (Composite map) Deposited 2022-02-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain i 2–128（127 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R43 Bovine complex I in the presence of IM1761092, active class iii (Composite map) Deposited 2022-02-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain i 2–128（127 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 CDL CARDIOLIPIN × 5 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R44 Bovine complex I in the presence of IM1761092, active class iv (Composite map) Deposited 2022-02-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain i 2–128（127 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R45 Bovine complex I in the presence of IM1761092, deactive class i (Composite map) Deposited 2022-02-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain i 2–128（127 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R46 Bovine complex I in the presence of IM1761092, deactive class ii (Composite map) Deposited 2022-02-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain i 2–128（127 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 11 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R47 Bovine complex I in the presence of IM1761092, deactive class iii (Composite map) Deposited 2022-02-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain i 2–128（127 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R48 Bovine complex I in the presence of IM1761092, deactive class iv (Composite map) Deposited 2022-02-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain i 2–128（127 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4C Bovine complex I in the presence of IM1761092, deactive class v (Composite map) Deposited 2022-02-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain i 2–128（127 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4D Bovine complex I in the presence of IM1761092, deactive class vi (Composite map) Deposited 2022-02-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain i 2–128（127 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4F Bovine complex I in the presence of IM1761092, slack class i (Composite map) Deposited 2022-02-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain i 2–128（127 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 7 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 CDL CARDIOLIPIN × 6 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R4G Bovine complex I in the presence of IM1761092, slack class ii (Composite map) Deposited 2022-02-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain i 2–128（127 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 10 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8Q0A Inward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain i 1–128（128 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 8 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0F Inward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain i 1–128（128 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 21 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0J Inward-facing, slack proteoliposome complex I at 3.8 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain i 1–128（128 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.80 Å
8Q0M Outward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain i 1–128（128 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0O Outward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain i 1–128（128 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0Q Outward-facing, slack proteoliposome complex I at 3.6 A. Initially purified in DDM Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain i 1–128（128 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.60 Å
8Q1P Inward-facing, open2 proteoliposome complex I at 2.9 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain i 1–128（128 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q1U Inward-facing, open1 proteoliposome complex I at 3.3 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain i 1–128（128 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.30 Å
8Q1Y Outward-facing, open2 proteoliposome complex I at 2.6 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain i 1–128（128 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q25 Outward-facing, open1 proteoliposome complex I at 2.8 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain i 1–128（128 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.80 Å
8Q45 Inward-facing, closed proteoliposome complex I at 2.7 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain i 1–128（128 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.70 Å
8Q46 Inward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain i 1–128（128 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q47 Inward-facing, open1 proteoliposome complex I at 2.9 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain i 1–128（128 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 26 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q48 Outward-facing, closed proteoliposome complex I at 2.5 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain i 1–128（128 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 35 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.50 Å
8Q49 Outward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain i 1–128（128 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 FVH [[(2~{R},3~{S},4~{R},5~{R})-5-[(3~{R})-3-aminocarbonylpiperidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2~{R},3~{R},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3-oxidanyl-4-phosphonooxy-oxolan-2-yl]methyl hydrogen phosphate × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q4A Outward-facing, open1 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain i 1–128（128 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
9SMF Reduced bovine complex I in lipid nanodisc, NADH-active-Q10 Deposited 2025-09-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain i 2–128（127 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.51 Å
9SMG Reduced bovine complex I in lipid nanodisc, NADH-active-altQ10 Deposited 2025-09-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain i 2–128（127 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.42 Å
9SMH Reduced bovine complex I in lipid nanodisc, NADH-active-DDM Deposited 2025-09-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain i 2–128（127 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.61 Å
9SMI Reduced bovine complex I in lipid nanodisc, NADH-deactive Deposited 2025-09-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain i 2–128（127 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 27 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 LMT DODECYL-BETA-D-MALTOSIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 CHD CHOLIC ACID × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.01 Å
9W2U Cryo-EM structure of complex I on the bovine heart submitochondrial particles, open Deposited 2025-07-28 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain u 2–128（127 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) FME N-FORMYLMETHIONINE × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å

49 other PDB entries and 49 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUB6_BOVIN
Isoform —
PDB entities 35
Chains and sequence ranges Author chain u； PDBConstruct 2–128； UniProt 2–128

NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial

Bos taurus

UniProt Q02374

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain j； UniProt 37–107 Not recorded NADH-ubiquinone oxidoreductase chain 3 × 1 （Q8WAB4） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （P42026） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （P23709） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （P17694） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P04394） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P25708） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P15690） NADH-ubiquinone oxidoreductase chain 1 × 1 （Q6EMT1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （P42028） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03924） NADH-ubiquinone oxidoreductase chain 4L × 1 （Q6EMS3） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03920） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03910） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03892） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （P34942） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （P34943） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q02375） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P23934） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q02370） Acyl carrier protein, mitochondrial × 1 （Q9CR21） Acyl carrier protein, mitochondrial × 1 （P52505） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （P23935） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q02366） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P42029） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q8HXG6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q95KV7） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （Q02377） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q02371） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q02376） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q02827） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q02379） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （Q02378） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q8HXG5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q02380） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q02367） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q02365） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q02372） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （P48305） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q02369） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q02368） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q02373） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （O97725） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q05752） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） FME N-FORMYLMETHIONINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 2.80 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9W2V Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
7DGQ Activity optimized supercomplex state1 Deposited 2020-11-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain Y 37–108（72 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 CU COPPER (II) ION × 3 HEA HEME-A × 2 MG MAGNESIUM ION × 1 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 UQ2 UBIQUINONE-2 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.00 Å
7DGR Activity optimized supercomplex state2 Deposited 2020-11-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain Y 37–108（72 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 3 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.60 Å
7DGS Activity optimized supercomplex state3 Deposited 2020-11-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain Y 37–108（72 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 7.80 Å
7DGZ Activity optimized complex I (closed form) Deposited 2020-11-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Y 37–108（72 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
7DH0 Activity optimized complex I (open form) Deposited 2020-11-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Y 37–108（72 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
7DKF Activity optimized supercomplex state4 Deposited 2020-11-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain Y2 37–108（72 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 8.30 Å
7QSD Bovine complex I in the active state at 3.1 A Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain j 1–108（108 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 4 LMT DODECYL-BETA-D-MALTOSIDE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7QSK Bovine complex I in lipid nanodisc, Active-Q10 Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain j 1–108（108 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.84 Å
7QSL Bovine complex I in lipid nanodisc, Active-apo Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain j 1–108（108 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.76 Å
7QSM Bovine complex I in lipid nanodisc, Deactive-ligand (composite) Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain j 1–108（108 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 GOL GLYCEROL × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.30 Å
7QSN Bovine complex I in lipid nanodisc, Deactive-apo Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain j 1–108（108 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.81 Å
7QSO Bovine complex I in lipid nanodisc, State 3 (Slack) Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain j 1–108（108 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 6 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 2 CDL CARDIOLIPIN × 6 U10 UBIQUINONE-10 × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.02 Å
7R41 Bovine complex I in the presence of IM1761092, active class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain j 1–108（108 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R42 Bovine complex I in the presence of IM1761092, active class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain j 1–108（108 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R43 Bovine complex I in the presence of IM1761092, active class iii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain j 1–108（108 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 CDL CARDIOLIPIN × 5 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R44 Bovine complex I in the presence of IM1761092, active class iv (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain j 1–108（108 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R45 Bovine complex I in the presence of IM1761092, deactive class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain j 1–108（108 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R46 Bovine complex I in the presence of IM1761092, deactive class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain j 1–108（108 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 11 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R47 Bovine complex I in the presence of IM1761092, deactive class iii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain j 1–108（108 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R48 Bovine complex I in the presence of IM1761092, deactive class iv (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain j 1–108（108 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4C Bovine complex I in the presence of IM1761092, deactive class v (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain j 1–108（108 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4D Bovine complex I in the presence of IM1761092, deactive class vi (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain j 1–108（108 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4F Bovine complex I in the presence of IM1761092, slack class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain j 1–108（108 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 7 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 CDL CARDIOLIPIN × 6 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R4G Bovine complex I in the presence of IM1761092, slack class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain j 1–108（108 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 10 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8Q0A Inward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain j 1–108（108 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 8 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0F Inward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain j 1–108（108 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 21 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0J Inward-facing, slack proteoliposome complex I at 3.8 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain j 1–108（108 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.80 Å
8Q0M Outward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain j 1–108（108 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0O Outward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain j 1–108（108 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0Q Outward-facing, slack proteoliposome complex I at 3.6 A. Initially purified in DDM Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain j 1–108（108 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.60 Å
8Q1P Inward-facing, open2 proteoliposome complex I at 2.9 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain j 1–108（108 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q1U Inward-facing, open1 proteoliposome complex I at 3.3 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain j 1–108（108 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.30 Å
8Q1Y Outward-facing, open2 proteoliposome complex I at 2.6 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain j 1–108（108 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q25 Outward-facing, open1 proteoliposome complex I at 2.8 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain j 1–108（108 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.80 Å
8Q45 Inward-facing, closed proteoliposome complex I at 2.7 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain j 1–108（108 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.70 Å
8Q46 Inward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain j 1–108（108 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q47 Inward-facing, open1 proteoliposome complex I at 2.9 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain j 1–108（108 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 26 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q48 Outward-facing, closed proteoliposome complex I at 2.5 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain j 1–108（108 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 35 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.50 Å
8Q49 Outward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain j 1–108（108 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 FVH [[(2~{R},3~{S},4~{R},5~{R})-5-[(3~{R})-3-aminocarbonylpiperidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2~{R},3~{R},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3-oxidanyl-4-phosphonooxy-oxolan-2-yl]methyl hydrogen phosphate × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q4A Outward-facing, open1 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain j 1–108（108 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
9SMF Reduced bovine complex I in lipid nanodisc, NADH-active-Q10 Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain j 1–108（108 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.51 Å
9SMG Reduced bovine complex I in lipid nanodisc, NADH-active-altQ10 Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain j 1–108（108 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.42 Å
9SMH Reduced bovine complex I in lipid nanodisc, NADH-active-DDM Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain j 1–108（108 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.61 Å
9SMI Reduced bovine complex I in lipid nanodisc, NADH-deactive Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain j 1–108（108 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 27 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 LMT DODECYL-BETA-D-MALTOSIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 CHD CHOLIC ACID × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.01 Å
9W2U Cryo-EM structure of complex I on the bovine heart submitochondrial particles, open Deposited 2025-07-28 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain j 37–107（71 aa）
Not recorded FME N-FORMYLMETHIONINE × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å

45 other PDB entries and 45 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUB2_BOVIN
Isoform —
PDB entities 36
Chains and sequence ranges Author chain j； PDBConstruct 1–71； UniProt 37–107

NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3

Bos taurus

UniProt Q02365

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain k； UniProt 12–92 Not recorded NADH-ubiquinone oxidoreductase chain 3 × 1 （Q8WAB4） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （P42026） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （P23709） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （P17694） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P04394） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P25708） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P15690） NADH-ubiquinone oxidoreductase chain 1 × 1 （Q6EMT1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （P42028） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03924） NADH-ubiquinone oxidoreductase chain 4L × 1 （Q6EMS3） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03920） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03910） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03892） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （P34942） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （P34943） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q02375） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P23934） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q02370） Acyl carrier protein, mitochondrial × 1 （Q9CR21） Acyl carrier protein, mitochondrial × 1 （P52505） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （P23935） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q02366） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P42029） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q8HXG6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q95KV7） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （Q02377） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q02371） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q02376） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q02827） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q02379） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （Q02378） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q8HXG5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q02380） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q02367） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q02374） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q02372） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （P48305） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q02369） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q02368） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q02373） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （O97725） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q05752） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） FME N-FORMYLMETHIONINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 2.80 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9W2V Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
7DGQ Activity optimized supercomplex state1 Deposited 2020-11-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain Z 1–98（98 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 CU COPPER (II) ION × 3 HEA HEME-A × 2 MG MAGNESIUM ION × 1 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 UQ2 UBIQUINONE-2 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.00 Å
7DGR Activity optimized supercomplex state2 Deposited 2020-11-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain Z 1–98（98 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 3 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.60 Å
7DGS Activity optimized supercomplex state3 Deposited 2020-11-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain Z 2–98（97 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 7.80 Å
7DGZ Activity optimized complex I (closed form) Deposited 2020-11-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Z 2–98（97 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
7DH0 Activity optimized complex I (open form) Deposited 2020-11-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain Z 2–98（97 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
7DKF Activity optimized supercomplex state4 Deposited 2020-11-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain Z2 2–98（97 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 8.30 Å
7QSD Bovine complex I in the active state at 3.1 A Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain k 1–98（98 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 4 LMT DODECYL-BETA-D-MALTOSIDE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7QSK Bovine complex I in lipid nanodisc, Active-Q10 Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain k 1–98（98 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.84 Å
7QSL Bovine complex I in lipid nanodisc, Active-apo Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain k 1–98（98 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.76 Å
7QSM Bovine complex I in lipid nanodisc, Deactive-ligand (composite) Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain k 1–98（98 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 GOL GLYCEROL × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.30 Å
7QSN Bovine complex I in lipid nanodisc, Deactive-apo Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain k 1–98（98 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.81 Å
7QSO Bovine complex I in lipid nanodisc, State 3 (Slack) Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain k 1–98（98 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 6 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 2 CDL CARDIOLIPIN × 6 U10 UBIQUINONE-10 × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.02 Å
7R41 Bovine complex I in the presence of IM1761092, active class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain k 1–98（98 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R42 Bovine complex I in the presence of IM1761092, active class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain k 1–98（98 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R43 Bovine complex I in the presence of IM1761092, active class iii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain k 1–98（98 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 CDL CARDIOLIPIN × 5 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R44 Bovine complex I in the presence of IM1761092, active class iv (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain k 1–98（98 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R45 Bovine complex I in the presence of IM1761092, deactive class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain k 1–98（98 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R46 Bovine complex I in the presence of IM1761092, deactive class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain k 1–98（98 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 11 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R47 Bovine complex I in the presence of IM1761092, deactive class iii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain k 1–98（98 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R48 Bovine complex I in the presence of IM1761092, deactive class iv (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain k 1–98（98 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4C Bovine complex I in the presence of IM1761092, deactive class v (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain k 1–98（98 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4D Bovine complex I in the presence of IM1761092, deactive class vi (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain k 1–98（98 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4F Bovine complex I in the presence of IM1761092, slack class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain k 1–98（98 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 7 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 CDL CARDIOLIPIN × 6 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R4G Bovine complex I in the presence of IM1761092, slack class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain k 1–98（98 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 10 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8Q0A Inward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain k 1–98（98 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 8 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0F Inward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain k 1–98（98 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 21 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0J Inward-facing, slack proteoliposome complex I at 3.8 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain k 1–98（98 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.80 Å
8Q0M Outward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain k 1–98（98 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0O Outward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain k 1–98（98 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0Q Outward-facing, slack proteoliposome complex I at 3.6 A. Initially purified in DDM Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain k 1–98（98 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.60 Å
8Q1P Inward-facing, open2 proteoliposome complex I at 2.9 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain k 1–98（98 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q1U Inward-facing, open1 proteoliposome complex I at 3.3 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain k 1–98（98 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.30 Å
8Q1Y Outward-facing, open2 proteoliposome complex I at 2.6 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain k 1–98（98 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q25 Outward-facing, open1 proteoliposome complex I at 2.8 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain k 1–98（98 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.80 Å
8Q45 Inward-facing, closed proteoliposome complex I at 2.7 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain k 1–98（98 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.70 Å
8Q46 Inward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain k 1–98（98 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q47 Inward-facing, open1 proteoliposome complex I at 2.9 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain k 1–98（98 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 26 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q48 Outward-facing, closed proteoliposome complex I at 2.5 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain k 1–98（98 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 35 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.50 Å
8Q49 Outward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain k 1–98（98 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 FVH [[(2~{R},3~{S},4~{R},5~{R})-5-[(3~{R})-3-aminocarbonylpiperidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2~{R},3~{R},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3-oxidanyl-4-phosphonooxy-oxolan-2-yl]methyl hydrogen phosphate × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q4A Outward-facing, open1 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain k 1–98（98 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
9SMF Reduced bovine complex I in lipid nanodisc, NADH-active-Q10 Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain k 1–98（98 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.51 Å
9SMG Reduced bovine complex I in lipid nanodisc, NADH-active-altQ10 Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain k 1–98（98 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.42 Å
9SMH Reduced bovine complex I in lipid nanodisc, NADH-active-DDM Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain k 1–98（98 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.61 Å
9SMI Reduced bovine complex I in lipid nanodisc, NADH-deactive Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain k 1–98（98 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 27 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 LMT DODECYL-BETA-D-MALTOSIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 CHD CHOLIC ACID × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.01 Å
9W2U Cryo-EM structure of complex I on the bovine heart submitochondrial particles, open Deposited 2025-07-28 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain k 12–92（81 aa）
Not recorded FME N-FORMYLMETHIONINE × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å

45 other PDB entries and 45 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUB3_BOVIN
Isoform —
PDB entities 37
Chains and sequence ranges Author chain k； PDBConstruct 1–81； UniProt 12–92

NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial

Bos taurus

UniProt Q02372

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain l； UniProt 31–186 Not recorded NADH-ubiquinone oxidoreductase chain 3 × 1 （Q8WAB4） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （P42026） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （P23709） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （P17694） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P04394） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P25708） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P15690） NADH-ubiquinone oxidoreductase chain 1 × 1 （Q6EMT1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （P42028） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03924） NADH-ubiquinone oxidoreductase chain 4L × 1 （Q6EMS3） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03920） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03910） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03892） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （P34942） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （P34943） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q02375） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P23934） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q02370） Acyl carrier protein, mitochondrial × 1 （Q9CR21） Acyl carrier protein, mitochondrial × 1 （P52505） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （P23935） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q02366） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P42029） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q8HXG6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q95KV7） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （Q02377） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q02371） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q02376） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q02827） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q02379） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （Q02378） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q8HXG5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q02380） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q02367） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q02374） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q02365） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （P48305） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q02369） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q02368） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q02373） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （O97725） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q05752） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） FME N-FORMYLMETHIONINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 2.80 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9W2V Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
7DGQ Activity optimized supercomplex state1 Deposited 2020-11-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain e 29–186（158 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 CU COPPER (II) ION × 3 HEA HEME-A × 2 MG MAGNESIUM ION × 1 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 UQ2 UBIQUINONE-2 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.00 Å
7DGR Activity optimized supercomplex state2 Deposited 2020-11-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain e 29–186（158 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 3 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.60 Å
7DGS Activity optimized supercomplex state3 Deposited 2020-11-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain e 29–186（158 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 7.80 Å
7DGZ Activity optimized complex I (closed form) Deposited 2020-11-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain e 29–186（158 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
7DH0 Activity optimized complex I (open form) Deposited 2020-11-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain e 29–186（158 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
7DKF Activity optimized supercomplex state4 Deposited 2020-11-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain e2 29–186（158 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 8.30 Å
7QSD Bovine complex I in the active state at 3.1 A Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain l 1–186（186 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 4 LMT DODECYL-BETA-D-MALTOSIDE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7QSK Bovine complex I in lipid nanodisc, Active-Q10 Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain l 1–186（186 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.84 Å
7QSL Bovine complex I in lipid nanodisc, Active-apo Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain l 1–186（186 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.76 Å
7QSM Bovine complex I in lipid nanodisc, Deactive-ligand (composite) Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain l 1–186（186 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 GOL GLYCEROL × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.30 Å
7QSN Bovine complex I in lipid nanodisc, Deactive-apo Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain l 1–186（186 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.81 Å
7QSO Bovine complex I in lipid nanodisc, State 3 (Slack) Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain l 1–186（186 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 6 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 2 CDL CARDIOLIPIN × 6 U10 UBIQUINONE-10 × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.02 Å
7R41 Bovine complex I in the presence of IM1761092, active class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain l 1–186（186 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R42 Bovine complex I in the presence of IM1761092, active class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain l 1–186（186 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R43 Bovine complex I in the presence of IM1761092, active class iii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain l 1–186（186 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 CDL CARDIOLIPIN × 5 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R44 Bovine complex I in the presence of IM1761092, active class iv (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain l 1–186（186 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R45 Bovine complex I in the presence of IM1761092, deactive class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain l 1–186（186 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R46 Bovine complex I in the presence of IM1761092, deactive class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain l 1–186（186 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 11 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R47 Bovine complex I in the presence of IM1761092, deactive class iii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain l 1–186（186 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R48 Bovine complex I in the presence of IM1761092, deactive class iv (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain l 1–186（186 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4C Bovine complex I in the presence of IM1761092, deactive class v (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain l 1–186（186 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4D Bovine complex I in the presence of IM1761092, deactive class vi (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain l 1–186（186 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4F Bovine complex I in the presence of IM1761092, slack class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain l 1–186（186 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 7 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 CDL CARDIOLIPIN × 6 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R4G Bovine complex I in the presence of IM1761092, slack class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain l 1–186（186 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 10 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8Q0A Inward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain l 1–186（186 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 8 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0F Inward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain l 1–186（186 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 21 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0J Inward-facing, slack proteoliposome complex I at 3.8 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain l 1–186（186 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.80 Å
8Q0M Outward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain l 1–186（186 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0O Outward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain l 1–186（186 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0Q Outward-facing, slack proteoliposome complex I at 3.6 A. Initially purified in DDM Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain l 1–186（186 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.60 Å
8Q1P Inward-facing, open2 proteoliposome complex I at 2.9 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain l 1–186（186 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q1U Inward-facing, open1 proteoliposome complex I at 3.3 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain l 1–186（186 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.30 Å
8Q1Y Outward-facing, open2 proteoliposome complex I at 2.6 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain l 1–186（186 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q25 Outward-facing, open1 proteoliposome complex I at 2.8 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain l 1–186（186 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.80 Å
8Q45 Inward-facing, closed proteoliposome complex I at 2.7 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain l 1–186（186 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.70 Å
8Q46 Inward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain l 1–186（186 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q47 Inward-facing, open1 proteoliposome complex I at 2.9 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain l 1–186（186 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 26 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q48 Outward-facing, closed proteoliposome complex I at 2.5 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain l 1–186（186 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 35 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.50 Å
8Q49 Outward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain l 1–186（186 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 FVH [[(2~{R},3~{S},4~{R},5~{R})-5-[(3~{R})-3-aminocarbonylpiperidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2~{R},3~{R},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3-oxidanyl-4-phosphonooxy-oxolan-2-yl]methyl hydrogen phosphate × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q4A Outward-facing, open1 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain l 1–186（186 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
9SMF Reduced bovine complex I in lipid nanodisc, NADH-active-Q10 Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain l 1–186（186 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.51 Å
9SMG Reduced bovine complex I in lipid nanodisc, NADH-active-altQ10 Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain l 1–186（186 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.42 Å
9SMH Reduced bovine complex I in lipid nanodisc, NADH-active-DDM Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain l 1–186（186 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.61 Å
9SMI Reduced bovine complex I in lipid nanodisc, NADH-deactive Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain l 1–186（186 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 27 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 LMT DODECYL-BETA-D-MALTOSIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 CHD CHOLIC ACID × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.01 Å
9W2U Cryo-EM structure of complex I on the bovine heart submitochondrial particles, open Deposited 2025-07-28 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain l 31–186（156 aa）
Not recorded FME N-FORMYLMETHIONINE × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å

45 other PDB entries and 45 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUB8_BOVIN
Isoform —
PDB entities 38
Chains and sequence ranges Author chain l； PDBConstruct 1–156； UniProt 31–186

NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4

Bos taurus

UniProt P48305

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain m； UniProt 2–129 Not recorded NADH-ubiquinone oxidoreductase chain 3 × 1 （Q8WAB4） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （P42026） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （P23709） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （P17694） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P04394） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P25708） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P15690） NADH-ubiquinone oxidoreductase chain 1 × 1 （Q6EMT1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （P42028） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03924） NADH-ubiquinone oxidoreductase chain 4L × 1 （Q6EMS3） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03920） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03910） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03892） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （P34942） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （P34943） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q02375） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P23934） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q02370） Acyl carrier protein, mitochondrial × 1 （Q9CR21） Acyl carrier protein, mitochondrial × 1 （P52505） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （P23935） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q02366） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P42029） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q8HXG6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q95KV7） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （Q02377） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q02371） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q02376） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q02827） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q02379） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （Q02378） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q8HXG5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q02380） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q02367） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q02374） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q02365） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q02372） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q02369） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q02368） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q02373） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （O97725） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q05752） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） FME N-FORMYLMETHIONINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 2.80 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9W2V Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5LC5 Structure of mammalian respiratory Complex I, class2 Deposited 2016-06-19 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain m 33–129（97 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 4.35 Å
5LDW Structure of mammalian respiratory Complex I, class1 Deposited 2016-06-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain m 33–129（97 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus present in a cold room, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 4.27 Å
5LDX Structure of mammalian respiratory Complex I, class3. Deposited 2016-06-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain m 33–129（97 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer 20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7；pH 8
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 5.60 Å
5O31 Mitochondrial complex I in the deactive state Deposited 2017-05-23 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain m 2–129（128 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.13 Å
7DGQ Activity optimized supercomplex state1 Deposited 2020-11-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain a 2–129（128 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 CU COPPER (II) ION × 3 HEA HEME-A × 2 MG MAGNESIUM ION × 1 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 UQ2 UBIQUINONE-2 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.00 Å
7DGR Activity optimized supercomplex state2 Deposited 2020-11-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain a 2–129（128 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 3 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.60 Å
7DGS Activity optimized supercomplex state3 Deposited 2020-11-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain a 2–129（128 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 7.80 Å
7DGZ Activity optimized complex I (closed form) Deposited 2020-11-12 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain a 2–129（128 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
7DH0 Activity optimized complex I (open form) Deposited 2020-11-12 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain a 2–129（128 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
7DKF Activity optimized supercomplex state4 Deposited 2020-11-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain a2 2–129（128 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 8.30 Å
7QSD Bovine complex I in the active state at 3.1 A Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain m 1–129（129 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 4 LMT DODECYL-BETA-D-MALTOSIDE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7QSK Bovine complex I in lipid nanodisc, Active-Q10 Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain m 1–129（129 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.84 Å
7QSL Bovine complex I in lipid nanodisc, Active-apo Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain m 1–129（129 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.76 Å
7QSM Bovine complex I in lipid nanodisc, Deactive-ligand (composite) Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain m 1–129（129 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 GOL GLYCEROL × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.30 Å
7QSN Bovine complex I in lipid nanodisc, Deactive-apo Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain m 1–129（129 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.81 Å
7QSO Bovine complex I in lipid nanodisc, State 3 (Slack) Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain m 1–129（129 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 6 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 2 CDL CARDIOLIPIN × 6 U10 UBIQUINONE-10 × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.02 Å
7R41 Bovine complex I in the presence of IM1761092, active class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain m 1–129（129 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R42 Bovine complex I in the presence of IM1761092, active class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain m 1–129（129 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R43 Bovine complex I in the presence of IM1761092, active class iii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain m 1–129（129 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 CDL CARDIOLIPIN × 5 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R44 Bovine complex I in the presence of IM1761092, active class iv (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain m 1–129（129 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R45 Bovine complex I in the presence of IM1761092, deactive class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain m 1–129（129 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R46 Bovine complex I in the presence of IM1761092, deactive class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain m 1–129（129 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 11 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R47 Bovine complex I in the presence of IM1761092, deactive class iii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain m 1–129（129 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R48 Bovine complex I in the presence of IM1761092, deactive class iv (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain m 1–129（129 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4C Bovine complex I in the presence of IM1761092, deactive class v (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain m 1–129（129 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4D Bovine complex I in the presence of IM1761092, deactive class vi (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain m 1–129（129 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4F Bovine complex I in the presence of IM1761092, slack class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain m 1–129（129 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 7 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 CDL CARDIOLIPIN × 6 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R4G Bovine complex I in the presence of IM1761092, slack class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain m 1–129（129 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 10 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8Q0A Inward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain m 1–129（129 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 8 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0F Inward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain m 1–129（129 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 21 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0J Inward-facing, slack proteoliposome complex I at 3.8 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain m 1–129（129 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.80 Å
8Q0M Outward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain m 1–129（129 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0O Outward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain m 1–129（129 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0Q Outward-facing, slack proteoliposome complex I at 3.6 A. Initially purified in DDM Deposited 2023-07-28 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain m 1–129（129 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.60 Å
8Q1P Inward-facing, open2 proteoliposome complex I at 2.9 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain m 1–129（129 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q1U Inward-facing, open1 proteoliposome complex I at 3.3 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain m 1–129（129 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.30 Å
8Q1Y Outward-facing, open2 proteoliposome complex I at 2.6 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain m 1–129（129 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q25 Outward-facing, open1 proteoliposome complex I at 2.8 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain m 1–129（129 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.80 Å
8Q45 Inward-facing, closed proteoliposome complex I at 2.7 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain m 1–129（129 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.70 Å
8Q46 Inward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain m 1–129（129 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q47 Inward-facing, open1 proteoliposome complex I at 2.9 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain m 1–129（129 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 26 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q48 Outward-facing, closed proteoliposome complex I at 2.5 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain m 1–129（129 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 35 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.50 Å
8Q49 Outward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain m 1–129（129 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 FVH [[(2~{R},3~{S},4~{R},5~{R})-5-[(3~{R})-3-aminocarbonylpiperidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2~{R},3~{R},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3-oxidanyl-4-phosphonooxy-oxolan-2-yl]methyl hydrogen phosphate × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q4A Outward-facing, open1 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain m 1–129（129 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
9SMF Reduced bovine complex I in lipid nanodisc, NADH-active-Q10 Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain m 1–129（129 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.51 Å
9SMG Reduced bovine complex I in lipid nanodisc, NADH-active-altQ10 Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain m 1–129（129 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.42 Å
9SMH Reduced bovine complex I in lipid nanodisc, NADH-active-DDM Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain m 1–129（129 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.61 Å
9SMI Reduced bovine complex I in lipid nanodisc, NADH-deactive Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain m 1–129（129 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 27 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 LMT DODECYL-BETA-D-MALTOSIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 CHD CHOLIC ACID × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.01 Å
9W2U Cryo-EM structure of complex I on the bovine heart submitochondrial particles, open Deposited 2025-07-28 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain m 2–129（128 aa）
Not recorded FME N-FORMYLMETHIONINE × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å

49 other PDB entries and 49 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUB4_BOVIN
Isoform —
PDB entities 39
Chains and sequence ranges Author chain m； PDBConstruct 1–128； UniProt 2–129

NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9

Bos taurus

UniProt Q02369

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain n； UniProt 8–179 Not recorded NADH-ubiquinone oxidoreductase chain 3 × 1 （Q8WAB4） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （P42026） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （P23709） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （P17694） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P04394） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P25708） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P15690） NADH-ubiquinone oxidoreductase chain 1 × 1 （Q6EMT1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （P42028） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03924） NADH-ubiquinone oxidoreductase chain 4L × 1 （Q6EMS3） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03920） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03910） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03892） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （P34942） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （P34943） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q02375） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P23934） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q02370） Acyl carrier protein, mitochondrial × 1 （Q9CR21） Acyl carrier protein, mitochondrial × 1 （P52505） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （P23935） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q02366） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P42029） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q8HXG6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q95KV7） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （Q02377） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q02371） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q02376） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q02827） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q02379） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （Q02378） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q8HXG5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q02380） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q02367） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q02374） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q02365） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q02372） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （P48305） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q02368） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q02373） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （O97725） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q05752） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） FME N-FORMYLMETHIONINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 2.80 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9W2V Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5LC5 Structure of mammalian respiratory Complex I, class2 Deposited 2016-06-19 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain n 11–137（127 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 4.35 Å
5LDW Structure of mammalian respiratory Complex I, class1 Deposited 2016-06-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain n 11–137（127 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus present in a cold room, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 4.27 Å
5LDX Structure of mammalian respiratory Complex I, class3. Deposited 2016-06-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain n 11–137（127 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer 20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7；pH 8
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 5.60 Å
5O31 Mitochondrial complex I in the deactive state Deposited 2017-05-23 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain n 2–179（178 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.13 Å
7DGQ Activity optimized supercomplex state1 Deposited 2020-11-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain f 2–179（178 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 CU COPPER (II) ION × 3 HEA HEME-A × 2 MG MAGNESIUM ION × 1 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 UQ2 UBIQUINONE-2 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.00 Å
7DGR Activity optimized supercomplex state2 Deposited 2020-11-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain f 2–179（178 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 3 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.60 Å
7DGS Activity optimized supercomplex state3 Deposited 2020-11-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain f 2–179（178 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 7.80 Å
7DGZ Activity optimized complex I (closed form) Deposited 2020-11-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain f 2–179（178 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
7DH0 Activity optimized complex I (open form) Deposited 2020-11-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain f 2–179（178 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
7DKF Activity optimized supercomplex state4 Deposited 2020-11-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain f2 2–179（178 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 8.30 Å
7QSD Bovine complex I in the active state at 3.1 A Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain n 1–179（179 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 4 LMT DODECYL-BETA-D-MALTOSIDE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7QSK Bovine complex I in lipid nanodisc, Active-Q10 Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain n 1–179（179 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.84 Å
7QSL Bovine complex I in lipid nanodisc, Active-apo Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain n 1–179（179 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.76 Å
7QSM Bovine complex I in lipid nanodisc, Deactive-ligand (composite) Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain n 1–179（179 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 GOL GLYCEROL × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.30 Å
7QSN Bovine complex I in lipid nanodisc, Deactive-apo Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain n 1–179（179 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.81 Å
7QSO Bovine complex I in lipid nanodisc, State 3 (Slack) Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain n 1–179（179 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 6 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 2 CDL CARDIOLIPIN × 6 U10 UBIQUINONE-10 × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.02 Å
7R41 Bovine complex I in the presence of IM1761092, active class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain n 1–179（179 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R42 Bovine complex I in the presence of IM1761092, active class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain n 1–179（179 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R43 Bovine complex I in the presence of IM1761092, active class iii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain n 1–179（179 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 CDL CARDIOLIPIN × 5 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R44 Bovine complex I in the presence of IM1761092, active class iv (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain n 1–179（179 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R45 Bovine complex I in the presence of IM1761092, deactive class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain n 1–179（179 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R46 Bovine complex I in the presence of IM1761092, deactive class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain n 1–179（179 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 11 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R47 Bovine complex I in the presence of IM1761092, deactive class iii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain n 1–179（179 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R48 Bovine complex I in the presence of IM1761092, deactive class iv (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain n 1–179（179 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4C Bovine complex I in the presence of IM1761092, deactive class v (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain n 1–179（179 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4D Bovine complex I in the presence of IM1761092, deactive class vi (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain n 1–179（179 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4F Bovine complex I in the presence of IM1761092, slack class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain n 1–179（179 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 7 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 CDL CARDIOLIPIN × 6 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R4G Bovine complex I in the presence of IM1761092, slack class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain n 1–179（179 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 10 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8Q0A Inward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain n 1–179（179 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 8 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0F Inward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain n 1–179（179 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 21 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0J Inward-facing, slack proteoliposome complex I at 3.8 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain n 1–179（179 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.80 Å
8Q0M Outward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain n 1–179（179 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0O Outward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain n 1–179（179 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0Q Outward-facing, slack proteoliposome complex I at 3.6 A. Initially purified in DDM Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain n 1–179（179 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.60 Å
8Q1P Inward-facing, open2 proteoliposome complex I at 2.9 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain n 1–179（179 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q1U Inward-facing, open1 proteoliposome complex I at 3.3 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain n 1–179（179 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.30 Å
8Q1Y Outward-facing, open2 proteoliposome complex I at 2.6 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain n 1–179（179 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q25 Outward-facing, open1 proteoliposome complex I at 2.8 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain n 1–179（179 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.80 Å
8Q45 Inward-facing, closed proteoliposome complex I at 2.7 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain n 1–179（179 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.70 Å
8Q46 Inward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain n 1–179（179 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q47 Inward-facing, open1 proteoliposome complex I at 2.9 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain n 1–179（179 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 26 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q48 Outward-facing, closed proteoliposome complex I at 2.5 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain n 1–179（179 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 35 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.50 Å
8Q49 Outward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain n 1–179（179 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 FVH [[(2~{R},3~{S},4~{R},5~{R})-5-[(3~{R})-3-aminocarbonylpiperidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2~{R},3~{R},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3-oxidanyl-4-phosphonooxy-oxolan-2-yl]methyl hydrogen phosphate × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q4A Outward-facing, open1 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain n 1–179（179 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
9SMF Reduced bovine complex I in lipid nanodisc, NADH-active-Q10 Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain n 1–179（179 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.51 Å
9SMG Reduced bovine complex I in lipid nanodisc, NADH-active-altQ10 Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain n 1–179（179 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.42 Å
9SMH Reduced bovine complex I in lipid nanodisc, NADH-active-DDM Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain n 1–179（179 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.61 Å
9SMI Reduced bovine complex I in lipid nanodisc, NADH-deactive Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain n 1–179（179 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 27 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 LMT DODECYL-BETA-D-MALTOSIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 CHD CHOLIC ACID × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.01 Å
9W2U Cryo-EM structure of complex I on the bovine heart submitochondrial particles, open Deposited 2025-07-28 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain n 8–179（172 aa）
Not recorded FME N-FORMYLMETHIONINE × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å

49 other PDB entries and 49 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUB9_BOVIN
Isoform —
PDB entities 40
Chains and sequence ranges Author chain n； PDBConstruct 1–172； UniProt 8–179

NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7

Bos taurus

UniProt Q02368

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain o； UniProt 2–123 Not recorded NADH-ubiquinone oxidoreductase chain 3 × 1 （Q8WAB4） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （P42026） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （P23709） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （P17694） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P04394） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P25708） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P15690） NADH-ubiquinone oxidoreductase chain 1 × 1 （Q6EMT1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （P42028） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03924） NADH-ubiquinone oxidoreductase chain 4L × 1 （Q6EMS3） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03920） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03910） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03892） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （P34942） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （P34943） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q02375） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P23934） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q02370） Acyl carrier protein, mitochondrial × 1 （Q9CR21） Acyl carrier protein, mitochondrial × 1 （P52505） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （P23935） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q02366） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P42029） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q8HXG6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q95KV7） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （Q02377） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q02371） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q02376） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q02827） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q02379） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （Q02378） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q8HXG5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q02380） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q02367） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q02374） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q02365） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q02372） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （P48305） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q02369） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q02373） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （O97725） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q05752） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） FME N-FORMYLMETHIONINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 2.80 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9W2V Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5LC5 Structure of mammalian respiratory Complex I, class2 Deposited 2016-06-19 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain o 58–115（58 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 4.35 Å
5LDW Structure of mammalian respiratory Complex I, class1 Deposited 2016-06-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain o 2–137（136 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus present in a cold room, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 4.27 Å
5LDX Structure of mammalian respiratory Complex I, class3. Deposited 2016-06-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain o 58–115（58 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer 20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7；pH 8
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 5.60 Å
5O31 Mitochondrial complex I in the deactive state Deposited 2017-05-23 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain o 2–137（136 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.13 Å
7DGQ Activity optimized supercomplex state1 Deposited 2020-11-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain d 2–118（117 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 CU COPPER (II) ION × 3 HEA HEME-A × 2 MG MAGNESIUM ION × 1 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 UQ2 UBIQUINONE-2 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.00 Å
7DGR Activity optimized supercomplex state2 Deposited 2020-11-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain d 2–118（117 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 3 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.60 Å
7DGS Activity optimized supercomplex state3 Deposited 2020-11-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain d 2–118（117 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 7.80 Å
7DGZ Activity optimized complex I (closed form) Deposited 2020-11-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain d 2–137（136 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
7DH0 Activity optimized complex I (open form) Deposited 2020-11-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain d 2–137（136 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
7DKF Activity optimized supercomplex state4 Deposited 2020-11-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain d2 2–137（136 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 8.30 Å
7QSD Bovine complex I in the active state at 3.1 A Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain o 1–137（137 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 4 LMT DODECYL-BETA-D-MALTOSIDE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7QSK Bovine complex I in lipid nanodisc, Active-Q10 Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain o 1–137（137 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.84 Å
7QSL Bovine complex I in lipid nanodisc, Active-apo Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain o 1–137（137 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.76 Å
7QSM Bovine complex I in lipid nanodisc, Deactive-ligand (composite) Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain o 1–137（137 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 GOL GLYCEROL × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.30 Å
7QSN Bovine complex I in lipid nanodisc, Deactive-apo Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain o 1–137（137 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.81 Å
7QSO Bovine complex I in lipid nanodisc, State 3 (Slack) Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain o 1–137（137 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 6 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 2 CDL CARDIOLIPIN × 6 U10 UBIQUINONE-10 × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.02 Å
7R41 Bovine complex I in the presence of IM1761092, active class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain o 1–137（137 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R42 Bovine complex I in the presence of IM1761092, active class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain o 1–137（137 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R43 Bovine complex I in the presence of IM1761092, active class iii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain o 1–137（137 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 CDL CARDIOLIPIN × 5 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R44 Bovine complex I in the presence of IM1761092, active class iv (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain o 1–137（137 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R45 Bovine complex I in the presence of IM1761092, deactive class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain o 1–137（137 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R46 Bovine complex I in the presence of IM1761092, deactive class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain o 1–137（137 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 11 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R47 Bovine complex I in the presence of IM1761092, deactive class iii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain o 1–137（137 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R48 Bovine complex I in the presence of IM1761092, deactive class iv (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain o 2–137（136 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4C Bovine complex I in the presence of IM1761092, deactive class v (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain o 1–137（137 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4D Bovine complex I in the presence of IM1761092, deactive class vi (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain o 1–137（137 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4F Bovine complex I in the presence of IM1761092, slack class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain o 1–137（137 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 7 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 CDL CARDIOLIPIN × 6 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R4G Bovine complex I in the presence of IM1761092, slack class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain o 1–137（137 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 10 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8Q0A Inward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain o 1–137（137 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 8 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0F Inward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain o 1–137（137 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 21 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0J Inward-facing, slack proteoliposome complex I at 3.8 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain o 1–137（137 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.80 Å
8Q0M Outward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain o 1–137（137 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0O Outward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain o 1–137（137 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0Q Outward-facing, slack proteoliposome complex I at 3.6 A. Initially purified in DDM Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain o 1–137（137 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.60 Å
8Q1P Inward-facing, open2 proteoliposome complex I at 2.9 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain o 1–137（137 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q1U Inward-facing, open1 proteoliposome complex I at 3.3 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain o 1–137（137 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.30 Å
8Q1Y Outward-facing, open2 proteoliposome complex I at 2.6 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain o 1–137（137 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q25 Outward-facing, open1 proteoliposome complex I at 2.8 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain o 1–137（137 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.80 Å
8Q45 Inward-facing, closed proteoliposome complex I at 2.7 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain o 1–137（137 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.70 Å
8Q46 Inward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain o 1–137（137 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q47 Inward-facing, open1 proteoliposome complex I at 2.9 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain o 1–137（137 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 26 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q48 Outward-facing, closed proteoliposome complex I at 2.5 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain o 1–137（137 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 35 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.50 Å
8Q49 Outward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain o 1–137（137 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 FVH [[(2~{R},3~{S},4~{R},5~{R})-5-[(3~{R})-3-aminocarbonylpiperidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2~{R},3~{R},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3-oxidanyl-4-phosphonooxy-oxolan-2-yl]methyl hydrogen phosphate × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q4A Outward-facing, open1 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain o 1–137（137 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
9SMF Reduced bovine complex I in lipid nanodisc, NADH-active-Q10 Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain o 1–137（137 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.51 Å
9SMG Reduced bovine complex I in lipid nanodisc, NADH-active-altQ10 Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain o 1–137（137 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.42 Å
9SMH Reduced bovine complex I in lipid nanodisc, NADH-active-DDM Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain o 1–137（137 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.61 Å
9SMI Reduced bovine complex I in lipid nanodisc, NADH-deactive Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain o 1–137（137 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 27 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 LMT DODECYL-BETA-D-MALTOSIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 CHD CHOLIC ACID × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.01 Å
9W2U Cryo-EM structure of complex I on the bovine heart submitochondrial particles, open Deposited 2025-07-28 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain o 2–123（122 aa）
Not recorded FME N-FORMYLMETHIONINE × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å

49 other PDB entries and 49 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUB7_BOVIN
Isoform —
PDB entities 41
Chains and sequence ranges Author chain o； PDBConstruct 1–122； UniProt 2–123

NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10

Bos taurus

UniProt Q02373

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain p； UniProt 3–175 Not recorded NADH-ubiquinone oxidoreductase chain 3 × 1 （Q8WAB4） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （P42026） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （P23709） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （P17694） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P04394） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P25708） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P15690） NADH-ubiquinone oxidoreductase chain 1 × 1 （Q6EMT1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （P42028） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03924） NADH-ubiquinone oxidoreductase chain 4L × 1 （Q6EMS3） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03920） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03910） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03892） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （P34942） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （P34943） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q02375） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P23934） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q02370） Acyl carrier protein, mitochondrial × 1 （Q9CR21） Acyl carrier protein, mitochondrial × 1 （P52505） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （P23935） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q02366） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P42029） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q8HXG6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q95KV7） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （Q02377） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q02371） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q02376） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q02827） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q02379） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （Q02378） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q8HXG5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q02380） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q02367） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q02374） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q02365） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q02372） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （P48305） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q02369） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q02368） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （O97725） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q05752） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） FME N-FORMYLMETHIONINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 2.80 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9W2V Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5LC5 Structure of mammalian respiratory Complex I, class2 Deposited 2016-06-19 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain p 77–143（67 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 4.35 Å
5LDW Structure of mammalian respiratory Complex I, class1 Deposited 2016-06-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain p 77–143（67 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus present in a cold room, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 4.27 Å
5LDX Structure of mammalian respiratory Complex I, class3. Deposited 2016-06-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain p 77–143（67 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer 20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7；pH 8
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 5.60 Å
5O31 Mitochondrial complex I in the deactive state Deposited 2017-05-23 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain p 77–143（67 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.13 Å
7DGQ Activity optimized supercomplex state1 Deposited 2020-11-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain g 1–176（176 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 CU COPPER (II) ION × 3 HEA HEME-A × 2 MG MAGNESIUM ION × 1 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 UQ2 UBIQUINONE-2 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.00 Å
7DGR Activity optimized supercomplex state2 Deposited 2020-11-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain g 1–176（176 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 3 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.60 Å
7DGS Activity optimized supercomplex state3 Deposited 2020-11-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain g 1–176（176 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 7.80 Å
7DGZ Activity optimized complex I (closed form) Deposited 2020-11-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain g 1–176（176 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
7DH0 Activity optimized complex I (open form) Deposited 2020-11-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain g 1–176（176 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
7DKF Activity optimized supercomplex state4 Deposited 2020-11-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain g2 1–176（176 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 8.30 Å
7QSD Bovine complex I in the active state at 3.1 A Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain p 1–176（176 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 4 LMT DODECYL-BETA-D-MALTOSIDE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7QSK Bovine complex I in lipid nanodisc, Active-Q10 Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain p 1–176（176 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.84 Å
7QSL Bovine complex I in lipid nanodisc, Active-apo Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain p 1–176（176 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.76 Å
7QSM Bovine complex I in lipid nanodisc, Deactive-ligand (composite) Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain p 1–176（176 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 GOL GLYCEROL × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.30 Å
7QSN Bovine complex I in lipid nanodisc, Deactive-apo Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain p 1–176（176 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.81 Å
7QSO Bovine complex I in lipid nanodisc, State 3 (Slack) Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain p 1–176（176 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 6 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 2 CDL CARDIOLIPIN × 6 U10 UBIQUINONE-10 × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.02 Å
7R41 Bovine complex I in the presence of IM1761092, active class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain p 1–176（176 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R42 Bovine complex I in the presence of IM1761092, active class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain p 1–176（176 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R43 Bovine complex I in the presence of IM1761092, active class iii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain p 1–176（176 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 CDL CARDIOLIPIN × 5 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R44 Bovine complex I in the presence of IM1761092, active class iv (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain p 1–176（176 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R45 Bovine complex I in the presence of IM1761092, deactive class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain p 1–176（176 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R46 Bovine complex I in the presence of IM1761092, deactive class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain p 1–176（176 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 11 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R47 Bovine complex I in the presence of IM1761092, deactive class iii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain p 1–176（176 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R48 Bovine complex I in the presence of IM1761092, deactive class iv (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain p 1–176（176 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4C Bovine complex I in the presence of IM1761092, deactive class v (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain p 1–176（176 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4D Bovine complex I in the presence of IM1761092, deactive class vi (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain p 1–176（176 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4F Bovine complex I in the presence of IM1761092, slack class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain p 1–176（176 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 7 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 CDL CARDIOLIPIN × 6 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R4G Bovine complex I in the presence of IM1761092, slack class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain p 1–176（176 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 10 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8Q0A Inward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain p 1–176（176 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 8 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0F Inward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain p 1–176（176 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 21 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0J Inward-facing, slack proteoliposome complex I at 3.8 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain p 1–176（176 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.80 Å
8Q0M Outward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain p 1–176（176 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0O Outward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain p 1–176（176 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0Q Outward-facing, slack proteoliposome complex I at 3.6 A. Initially purified in DDM Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain p 1–176（176 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.60 Å
8Q1P Inward-facing, open2 proteoliposome complex I at 2.9 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain p 1–176（176 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q1U Inward-facing, open1 proteoliposome complex I at 3.3 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain p 1–176（176 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.30 Å
8Q1Y Outward-facing, open2 proteoliposome complex I at 2.6 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain p 1–176（176 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q25 Outward-facing, open1 proteoliposome complex I at 2.8 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain p 1–176（176 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.80 Å
8Q45 Inward-facing, closed proteoliposome complex I at 2.7 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain p 1–176（176 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.70 Å
8Q46 Inward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain p 1–176（176 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q47 Inward-facing, open1 proteoliposome complex I at 2.9 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain p 1–176（176 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 26 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q48 Outward-facing, closed proteoliposome complex I at 2.5 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain p 1–176（176 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 35 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.50 Å
8Q49 Outward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain p 1–176（176 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 FVH [[(2~{R},3~{S},4~{R},5~{R})-5-[(3~{R})-3-aminocarbonylpiperidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2~{R},3~{R},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3-oxidanyl-4-phosphonooxy-oxolan-2-yl]methyl hydrogen phosphate × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q4A Outward-facing, open1 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain p 1–176（176 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
9SMF Reduced bovine complex I in lipid nanodisc, NADH-active-Q10 Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain p 1–176（176 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.51 Å
9SMG Reduced bovine complex I in lipid nanodisc, NADH-active-altQ10 Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain p 1–176（176 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.42 Å
9SMH Reduced bovine complex I in lipid nanodisc, NADH-active-DDM Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain p 1–176（176 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.61 Å
9SMI Reduced bovine complex I in lipid nanodisc, NADH-deactive Deposited 2025-09-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain p 1–176（176 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 27 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 LMT DODECYL-BETA-D-MALTOSIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 CHD CHOLIC ACID × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.01 Å
9W2U Cryo-EM structure of complex I on the bovine heart submitochondrial particles, open Deposited 2025-07-28 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain p 3–175（173 aa）
Not recorded FME N-FORMYLMETHIONINE × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å

49 other PDB entries and 49 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUBA_BOVIN
Isoform —
PDB entities 42
Chains and sequence ranges Author chain p； PDBConstruct 1–173； UniProt 3–175

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12

Bos taurus

UniProt O97725

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain q； UniProt 1–145 Not recorded NADH-ubiquinone oxidoreductase chain 3 × 1 （Q8WAB4） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （P42026） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （P23709） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （P17694） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P04394） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P25708） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P15690） NADH-ubiquinone oxidoreductase chain 1 × 1 （Q6EMT1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （P42028） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03924） NADH-ubiquinone oxidoreductase chain 4L × 1 （Q6EMS3） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03920） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03910） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03892） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （P34942） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （P34943） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q02375） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P23934） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q02370） Acyl carrier protein, mitochondrial × 1 （Q9CR21） Acyl carrier protein, mitochondrial × 1 （P52505） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （P23935） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q02366） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P42029） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q8HXG6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q95KV7） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （Q02377） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q02371） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q02376） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q02827） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q02379） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （Q02378） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q8HXG5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q02380） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q02367） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q02374） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q02365） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q02372） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （P48305） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q02369） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q02368） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q02373） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q05752） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） FME N-FORMYLMETHIONINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 2.80 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9W2V Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5LC5 Structure of mammalian respiratory Complex I, class2 Deposited 2016-06-19 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain q 2–139（138 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 4.35 Å
5LDW Structure of mammalian respiratory Complex I, class1 Deposited 2016-06-28 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain q 1–145（145 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus present in a cold room, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 4.27 Å
5LDX Structure of mammalian respiratory Complex I, class3. Deposited 2016-06-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain q 2–139（138 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer 20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7；pH 8
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 5.60 Å
5O31 Mitochondrial complex I in the deactive state Deposited 2017-05-23 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain q 1–145（145 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.13 Å
7DGQ Activity optimized supercomplex state1 Deposited 2020-11-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain U 1–145（145 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 CU COPPER (II) ION × 3 HEA HEME-A × 2 MG MAGNESIUM ION × 1 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 UQ2 UBIQUINONE-2 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.00 Å
7DGR Activity optimized supercomplex state2 Deposited 2020-11-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain U 1–145（145 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 3 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.60 Å
7DGS Activity optimized supercomplex state3 Deposited 2020-11-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain U 1–145（145 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 7.80 Å
7DGZ Activity optimized complex I (closed form) Deposited 2020-11-12 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain U 1–145（145 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
7DH0 Activity optimized complex I (open form) Deposited 2020-11-12 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain U 1–145（145 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
7DKF Activity optimized supercomplex state4 Deposited 2020-11-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain U2 1–145（145 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 8.30 Å
7QSD Bovine complex I in the active state at 3.1 A Deposited 2022-01-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain q 1–145（145 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 4 LMT DODECYL-BETA-D-MALTOSIDE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7QSK Bovine complex I in lipid nanodisc, Active-Q10 Deposited 2022-01-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain q 1–145（145 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.84 Å
7QSL Bovine complex I in lipid nanodisc, Active-apo Deposited 2022-01-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain q 1–145（145 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.76 Å
7QSM Bovine complex I in lipid nanodisc, Deactive-ligand (composite) Deposited 2022-01-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain q 1–145（145 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 GOL GLYCEROL × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.30 Å
7QSN Bovine complex I in lipid nanodisc, Deactive-apo Deposited 2022-01-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain q 1–145（145 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.81 Å
7QSO Bovine complex I in lipid nanodisc, State 3 (Slack) Deposited 2022-01-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain q 1–145（145 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 6 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 2 CDL CARDIOLIPIN × 6 U10 UBIQUINONE-10 × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.02 Å
7R41 Bovine complex I in the presence of IM1761092, active class i (Composite map) Deposited 2022-02-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain q 1–145（145 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R42 Bovine complex I in the presence of IM1761092, active class ii (Composite map) Deposited 2022-02-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain q 1–145（145 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R43 Bovine complex I in the presence of IM1761092, active class iii (Composite map) Deposited 2022-02-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain q 1–145（145 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 CDL CARDIOLIPIN × 5 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R44 Bovine complex I in the presence of IM1761092, active class iv (Composite map) Deposited 2022-02-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain q 1–145（145 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R45 Bovine complex I in the presence of IM1761092, deactive class i (Composite map) Deposited 2022-02-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain q 1–145（145 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R46 Bovine complex I in the presence of IM1761092, deactive class ii (Composite map) Deposited 2022-02-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain q 1–145（145 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 11 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R47 Bovine complex I in the presence of IM1761092, deactive class iii (Composite map) Deposited 2022-02-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain q 1–145（145 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R48 Bovine complex I in the presence of IM1761092, deactive class iv (Composite map) Deposited 2022-02-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain q 1–145（145 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4C Bovine complex I in the presence of IM1761092, deactive class v (Composite map) Deposited 2022-02-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain q 1–145（145 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4D Bovine complex I in the presence of IM1761092, deactive class vi (Composite map) Deposited 2022-02-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain q 1–145（145 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4F Bovine complex I in the presence of IM1761092, slack class i (Composite map) Deposited 2022-02-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain q 1–145（145 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 7 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 CDL CARDIOLIPIN × 6 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R4G Bovine complex I in the presence of IM1761092, slack class ii (Composite map) Deposited 2022-02-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain q 1–145（145 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 10 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8Q0A Inward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain q 1–145（145 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 8 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0F Inward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain q 1–145（145 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 21 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0J Inward-facing, slack proteoliposome complex I at 3.8 A. Initially purified in DDM. Deposited 2023-07-28 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain q 1–145（145 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.80 Å
8Q0M Outward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain q 1–145（145 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0O Outward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain q 1–145（145 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0Q Outward-facing, slack proteoliposome complex I at 3.6 A. Initially purified in DDM Deposited 2023-07-28 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain q 1–145（145 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.60 Å
8Q1P Inward-facing, open2 proteoliposome complex I at 2.9 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain q 1–145（145 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q1U Inward-facing, open1 proteoliposome complex I at 3.3 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain q 1–145（145 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.30 Å
8Q1Y Outward-facing, open2 proteoliposome complex I at 2.6 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain q 1–145（145 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q25 Outward-facing, open1 proteoliposome complex I at 2.8 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different mutation/modification Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain q 1–145（145 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.80 Å
8Q45 Inward-facing, closed proteoliposome complex I at 2.7 A. Initially purified in LMNG. Deposited 2023-08-05 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain q 1–145（145 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.70 Å
8Q46 Inward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain q 1–145（145 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q47 Inward-facing, open1 proteoliposome complex I at 2.9 A. Initially purified in LMNG. Deposited 2023-08-05 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain q 1–145（145 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 26 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q48 Outward-facing, closed proteoliposome complex I at 2.5 A. Initially purified in LMNG. Deposited 2023-08-05 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain q 1–145（145 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 35 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.50 Å
8Q49 Outward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain q 1–145（145 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 FVH [[(2~{R},3~{S},4~{R},5~{R})-5-[(3~{R})-3-aminocarbonylpiperidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2~{R},3~{R},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3-oxidanyl-4-phosphonooxy-oxolan-2-yl]methyl hydrogen phosphate × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q4A Outward-facing, open1 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain q 1–145（145 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
9SMF Reduced bovine complex I in lipid nanodisc, NADH-active-Q10 Deposited 2025-09-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain q 1–145（145 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.51 Å
9SMG Reduced bovine complex I in lipid nanodisc, NADH-active-altQ10 Deposited 2025-09-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain q 1–145（145 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.42 Å
9SMH Reduced bovine complex I in lipid nanodisc, NADH-active-DDM Deposited 2025-09-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain q 1–145（145 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.61 Å
9SMI Reduced bovine complex I in lipid nanodisc, NADH-deactive Deposited 2025-09-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain q 1–145（145 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 27 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 LMT DODECYL-BETA-D-MALTOSIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 CHD CHOLIC ACID × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.01 Å
9W2U Cryo-EM structure of complex I on the bovine heart submitochondrial particles, open Deposited 2025-07-28 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain q 1–145（145 aa）
Not recorded FME N-FORMYLMETHIONINE × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å

49 other PDB entries and 49 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUAC_BOVIN
Isoform —
PDB entities 43
Chains and sequence ranges Author chain q； PDBConstruct 1–145； UniProt 1–145

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7

Bos taurus

UniProt Q05752

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain v； UniProt 2–113 Non-standard monomer：Yes (specific site not provided by mmCIF) NADH-ubiquinone oxidoreductase chain 3 × 1 （Q8WAB4） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （P42026） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （P23709） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （P17694） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P04394） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P25708） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P15690） NADH-ubiquinone oxidoreductase chain 1 × 1 （Q6EMT1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （P42028） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03924） NADH-ubiquinone oxidoreductase chain 4L × 1 （Q6EMS3） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03920） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03910） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03892） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （P34942） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （P34943） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q02375） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P23934） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q02370） Acyl carrier protein, mitochondrial × 1 （Q9CR21） Acyl carrier protein, mitochondrial × 1 （P52505） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （P23935） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q02366） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P42029） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q8HXG6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q95KV7） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （Q02377） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q02371） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q02376） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q02827） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q02379） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （Q02378） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q8HXG5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q02380） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q02367） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q02374） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q02365） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q02372） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （P48305） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q02369） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q02368） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q02373） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （O97725） NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial × 1 （P56181） FME N-FORMYLMETHIONINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 2.80 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9W2V Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5O31 Mitochondrial complex I in the deactive state Deposited 2017-05-23 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain r 2–113（112 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.13 Å
7DGQ Activity optimized supercomplex state1 Deposited 2020-11-12 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain P 2–113（112 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 CU COPPER (II) ION × 3 HEA HEME-A × 2 MG MAGNESIUM ION × 1 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 UQ2 UBIQUINONE-2 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.00 Å
7DGR Activity optimized supercomplex state2 Deposited 2020-11-12 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain P 2–113（112 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 3 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.60 Å
7DGS Activity optimized supercomplex state3 Deposited 2020-11-12 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain P 2–113（112 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 7.80 Å
7DGZ Activity optimized complex I (closed form) Deposited 2020-11-12 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain P 2–113（112 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
7DH0 Activity optimized complex I (open form) Deposited 2020-11-12 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain P 2–113（112 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
7DKF Activity optimized supercomplex state4 Deposited 2020-11-24 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain P2 2–113（112 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 8.30 Å
7QSD Bovine complex I in the active state at 3.1 A Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain r 1–113（113 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 4 LMT DODECYL-BETA-D-MALTOSIDE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7QSK Bovine complex I in lipid nanodisc, Active-Q10 Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain r 1–113（113 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.84 Å
7QSL Bovine complex I in lipid nanodisc, Active-apo Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain r 1–113（113 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.76 Å
7QSM Bovine complex I in lipid nanodisc, Deactive-ligand (composite) Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain r 1–113（113 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 GOL GLYCEROL × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.30 Å
7QSN Bovine complex I in lipid nanodisc, Deactive-apo Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain r 1–113（113 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.81 Å
7QSO Bovine complex I in lipid nanodisc, State 3 (Slack) Deposited 2022-01-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain r 1–113（113 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 6 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 2 CDL CARDIOLIPIN × 6 U10 UBIQUINONE-10 × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.02 Å
7R41 Bovine complex I in the presence of IM1761092, active class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain r 1–113（113 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R42 Bovine complex I in the presence of IM1761092, active class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain r 1–113（113 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R43 Bovine complex I in the presence of IM1761092, active class iii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain r 1–113（113 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 CDL CARDIOLIPIN × 5 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R44 Bovine complex I in the presence of IM1761092, active class iv (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain r 1–113（113 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R45 Bovine complex I in the presence of IM1761092, deactive class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain r 1–113（113 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R46 Bovine complex I in the presence of IM1761092, deactive class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain r 1–113（113 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 11 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R47 Bovine complex I in the presence of IM1761092, deactive class iii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain r 1–113（113 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R48 Bovine complex I in the presence of IM1761092, deactive class iv (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain r 1–113（113 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4C Bovine complex I in the presence of IM1761092, deactive class v (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain r 1–113（113 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4D Bovine complex I in the presence of IM1761092, deactive class vi (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain r 1–113（113 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4F Bovine complex I in the presence of IM1761092, slack class i (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain r 1–113（113 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) LMT DODECYL-BETA-D-MALTOSIDE × 7 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 CDL CARDIOLIPIN × 6 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R4G Bovine complex I in the presence of IM1761092, slack class ii (Composite map) Deposited 2022-02-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain r 1–113（113 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 10 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8Q0A Inward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain r 1–113（113 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 8 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0F Inward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain r 1–113（113 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 21 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0J Inward-facing, slack proteoliposome complex I at 3.8 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain r 1–113（113 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.80 Å
8Q0M Outward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain r 1–113（113 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0O Outward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain r 1–113（113 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0Q Outward-facing, slack proteoliposome complex I at 3.6 A. Initially purified in DDM Deposited 2023-07-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain r 1–113（113 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.60 Å
8Q1P Inward-facing, open2 proteoliposome complex I at 2.9 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain r 1–113（113 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q1U Inward-facing, open1 proteoliposome complex I at 3.3 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain r 1–113（113 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.30 Å
8Q1Y Outward-facing, open2 proteoliposome complex I at 2.6 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain r 1–113（113 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q25 Outward-facing, open1 proteoliposome complex I at 2.8 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Different construct Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain r 1–113（113 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.80 Å
8Q45 Inward-facing, closed proteoliposome complex I at 2.7 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain r 1–113（113 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.70 Å
8Q46 Inward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain r 1–113（113 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q47 Inward-facing, open1 proteoliposome complex I at 2.9 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain r 1–113（113 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 26 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q48 Outward-facing, closed proteoliposome complex I at 2.5 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain r 1–113（113 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 35 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.50 Å
8Q49 Outward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain r 1–113（113 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 FVH [[(2~{R},3~{S},4~{R},5~{R})-5-[(3~{R})-3-aminocarbonylpiperidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2~{R},3~{R},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3-oxidanyl-4-phosphonooxy-oxolan-2-yl]methyl hydrogen phosphate × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q4A Outward-facing, open1 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain r 1–113（113 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
9SMF Reduced bovine complex I in lipid nanodisc, NADH-active-Q10 Deposited 2025-09-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain r 2–113（112 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.51 Å
9SMG Reduced bovine complex I in lipid nanodisc, NADH-active-altQ10 Deposited 2025-09-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain r 2–113（112 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.42 Å
9SMH Reduced bovine complex I in lipid nanodisc, NADH-active-DDM Deposited 2025-09-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain r 2–113（112 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.61 Å
9SMI Reduced bovine complex I in lipid nanodisc, NADH-deactive Deposited 2025-09-08 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain r 2–113（112 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 27 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 LMT DODECYL-BETA-D-MALTOSIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 CHD CHOLIC ACID × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.01 Å
9W2U Cryo-EM structure of complex I on the bovine heart submitochondrial particles, open Deposited 2025-07-28 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain v 2–113（112 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) FME N-FORMYLMETHIONINE × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å

46 other PDB entries and 46 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUA7_BOVIN
Isoform —
PDB entities 44
Chains and sequence ranges Author chain v； PDBConstruct 2–113； UniProt 2–113

NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial

Bos taurus

UniProt P56181

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 45 PDB declaration: 45-meric（45） Consistent with protein copy count Chain s； UniProt 64–108 Not recorded NADH-ubiquinone oxidoreductase chain 3 × 1 （Q8WAB4） NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial × 1 （P42026） NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial × 1 （P23709） NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial × 1 （P17694） NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial × 1 （P04394） NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial × 1 （P25708） NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial × 1 （P15690） NADH-ubiquinone oxidoreductase chain 1 × 1 （Q6EMT1） NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial × 1 （P42028） NADH-ubiquinone oxidoreductase chain 6 × 1 （P03924） NADH-ubiquinone oxidoreductase chain 4L × 1 （Q6EMS3） NADH-ubiquinone oxidoreductase chain 5 × 1 （P03920） NADH-ubiquinone oxidoreductase chain 4 × 1 （P03910） NADH-ubiquinone oxidoreductase chain 2 × 1 （P03892） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial × 1 （P34942） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial × 1 （P34943） NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial × 1 （Q02375） NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial × 1 （P23934） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 × 1 （Q02370） Acyl carrier protein, mitochondrial × 1 （Q9CR21） Acyl carrier protein, mitochondrial × 1 （P52505） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 × 1 （P23935） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 × 1 （Q02366） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 × 1 （P42029） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 × 1 （Q8HXG6） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 × 1 （Q95KV7） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 × 1 （Q02377） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 × 1 （Q02371） NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial × 1 （Q02376） NADH dehydrogenase [ubiquinone] 1 subunit C2 × 1 （Q02827） NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 × 1 （Q02379） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 × 1 （Q02378） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial × 1 （Q8HXG5） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial × 1 （Q02380） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 × 1 （Q02367） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial × 1 （Q02374） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 × 1 （Q02365） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial × 1 （Q02372） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 × 1 （P48305） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 × 1 （Q02369） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 × 1 （Q02368） NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 × 1 （Q02373） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 × 1 （O97725） NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 × 1 （Q05752） FME N-FORMYLMETHIONINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 2.80 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9W2V Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5XTB Cryo-EM structure of human respiratory complex I matrix arm Deposited 2017-06-18 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain K 74–106（33 aa） Fragment：UNP RESIDUES 74-106
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5XTD Cryo-EM structure of human respiratory complex I Deposited 2017-06-19 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain K 74–106（33 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 9 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 CDL CARDIOLIPIN × 5 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
5XTH Cryo-EM structure of human respiratory supercomplex I1III2IV1 Deposited 2017-06-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric
Chain K 74–106（33 aa） Fragment：UNP RESIDUES 74-106
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 12 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 4 CDL CARDIOLIPIN × 14 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 10 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 HEA HEME-A × 2 ZN ZINC ION × 1 HEC HEME C × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
5XTI Cryo-EM architecture of human respiratory chain megacomplex-I2III2IV2 Deposited 2017-06-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 138 PDB declaration: 138-meric
Chain BK 74–106（33 aa） Fragment：UNP RESIDUES 74-106
Chain K 74–106（33 aa） Fragment：UNP RESIDUES 74-106
Not recorded SF4 IRON/SULFUR CLUSTER × 12 FMN FLAVIN MONONUCLEOTIDE × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 21 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 4 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 6 CDL CARDIOLIPIN × 19 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 14 CU COPPER (II) ION × 6 MG MAGNESIUM ION × 2 HEA HEME-A × 4 ZN ZINC ION × 2 HEC HEME C × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 17.40 Å
9CWT Human kidney respiratory complex I Deposited 2024-07-30 Different construct Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain K 1–108（108 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 ZMP S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.44 Å
9I4I High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-01-24 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain K 1–108（108 aa）
Not recorded PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 CDL CARDIOLIPIN × 6 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.63 Å
9TI4 High resolution Cryo-EM structure of human complex I in mitochondria Deposited 2025-12-04 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain K 68–106（39 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 7 8Q1 S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate × 2 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 7 ZN ZINC ION × 1 CDL CARDIOLIPIN × 6 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；25 mM HEPES-KOH pH=7.5, 50 mM KCl, 20 mM Mg(OAc)2, 0.01% (v/v) Lauryl Maltose Neopentyl Glycol (LMNG), 0.001 % cardiolipin, 0.001 % glycol-diosgenin (GDN, Sigma-Aldrich), 0.1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.66 Å
9W2U Cryo-EM structure of complex I on the bovine heart submitochondrial particles, open Deposited 2025-07-28 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric
Chain s 64–108（45 aa）
Not recorded FME N-FORMYLMETHIONINE × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å

8 other PDB entries and 8 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NDUV3_HUMAN
Isoform —
PDB entities 45
Chains and sequence ranges Author chain s； PDBConstruct 1–45； UniProt 64–108

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 9w2v

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 9w2v
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2. Structure Basics 2. Structure Basics

Entry ID entry_id9w2v
Deposition date deposition_date2025-07-28
Structure title titleCryo-EM structure of complex I on the bovine heart submitochondrial particles, closed
Keywords keywordsrespiratory chain complex, supercomplex, submitochondrial particles, MOTOR PROTEIN, OXIDOREDUCTASE; OXIDOREDUCTASE
Experimental Method methodELECTRON MICROSCOPY
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier83.00 Å
Radius of gyration Rg (electron density) rg_electron82.97 Å
Forward intensity I(0) i012018800000.00
Molecular weight molecular_weight976150.0 kDa
Excluded volume excluded_volume1238600 ų
Envelope volume envelope_volume1849500 ų
Hydration-shell volume shell_volume189350 ų
Envelope diameter envelope_diameter308.6 Å
Shell Rg shell_rg76.81 Å
Envelope Rg envelope_rg82.20 Å
Shape Rg shape_rg83.03 Å
Total Rg total_rg82.70 Å
Total atoms total_atoms68503
Residues n_residues8281
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax221.3 Å
Rg (real space) rg_real78.80 Å
Rg uncertainty (real space) rg_real_error0.82 Å
I(0) (real space) i0_real1.1600e+10
I(0) uncertainty (real space) i0_real_error2.2240e+08
Rg (reciprocal space) rg_reciprocal80.62 Å
I(0) (reciprocal space) i0_reciprocal11940000000.0000
Solution quality estimate total_estimate0.9068
Solution quality rating solution_quality EXCELLENT a EXCELLENT solution
P(r) peaks n_peaks1
Primary peak position r_peak_primary81.5 Å
Skewness Skewness skewness0.325
Kurtosis Kurtosis kurtosis-0.713
Angular range angular_range— – 0.0950 Å−1
Current regularization parameter α current_alpha0.1874
Highest regularization parameter α highest_alpha815000000.0000
Real-space data points n_real_points20
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.976; Stabil: 0.984; Sysdev: 1.000; Positv: 1.000; Valcen: 1.000; Smooth: 0.000
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (57)

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8. Citations (1)

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9. Files and Curves (10)