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9czq

Ca2+ bound open-inactivated hSlo1 + beta2N-beta4 channel in detergent.

Method: ELECTRON MICROSCOPY Dmax: 172.2 Å Quality: GOOD
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Isoform 5 of Calcium-activated potassium channel subunit alpha-1

Homo sapiens

UniProt Q12791

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Insufficient information Heteromer Protein × 8 PDB declaration: octameric（8） Consistent with protein copy count Chain A； UniProt 66–1121 Chain B； UniProt 66–1121 Chain C； UniProt 66–1121 Chain D； UniProt 66–1121 Not recorded Large-conductance Ca2+-activated K+ channel beta2 subunit,Calcium-activated potassium channel subunit beta-4 × 4 （B5BNX0,Q86W47） MG MAGNESIUM ION × 4 CA CALCIUM ION × 8 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 20 CLR CHOLESTEROL × 11 K POTASSIUM ION × 4 ELECTRON MICROSCOPY cryo-EM buffer：pH 8；20 mM Tris-HCl pH 8.0, 450 mM KCl, 5 mM EDTA, 15 mM MgCl2, 0.02% GDN and 0.05 mg/ml POPE:POPC:POPA 5:5:1 (w:w:w). cryo-EM vitrification conditions：Cryogen ETHANE Resolution 2.88 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9CZQ Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
10AD Cryo-EM structure of the human BK channel bound to the agonist NS1619 Deposited 2026-01-08 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 66–1178（1113 aa）
Chain C 66–1178（1113 aa）
Chain D 66–1178（1113 aa）
Chain E 66–1178（1113 aa）
Not recorded MG MAGNESIUM ION × 4 CA CALCIUM ION × 8 A1E02 3-[2-oxidanyl-5-(trifluoromethyl)phenyl]-6-(trifluoromethyl)-1~{H}-benzimidazol-2-one × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.44 Å
2K44 Solution structure of a K+-channel voltage-sensor paddle domain Deposited 2008-05-28 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 257–284（28 aa）
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 5.5；310 K；Ionic strength (raw mmCIF value) 0.05；Pressure ambient
NMR sample composition 100mM [U-2H] 98% DPC, 50mM potassium chloride, 1mM HsapBK(233-260), 90% H2O/10% D2O | 90% H2O/10% D2O
Resolution not provided
3NAF Structure of the Intracellular Gating Ring from the Human High-conductance Ca2+ gated K+ Channel (BK Channel) Deposited 2010-06-01 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 394–681（288 aa） Fragment：Intracellular Domain (UNP RESIDUES 329-616; 659-1113)
Chain A 786–1240（455 aa） Fragment：Intracellular Domain (UNP RESIDUES 329-616; 659-1113)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；277 K；3.5M sodium formate, 0.1M Tris-HCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K
Resolution 3.10 Å R-free 0.289
6ND0 human BK channel reconstituted into liposomes Deposited 2018-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 292–1175（884 aa）
Chain B 292–1175（884 aa）
Chain C 292–1175（884 aa）
Chain D 292–1175（884 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.3
cryo-EM vitrification conditions Cryogen ETHANE；BK proteoliposomes were applied onto a glow-discharged perforated carbon grid (CFlat R2/2, EMS), and incubated for 5-10 min In an FEI Vitrobot Mark IV (FEI). After incubation, the sample was blotted from the side and buffer containing 10 mM HEPES, pH 7.3, 75 mM KCl, and 1 mM EDTA was applied onto the TEM grid to further swell proteoliposomes. Then grids were blotted for 7 s with force 0 and fast frozen in liquid ethane cooled by liquid nitrogen.
Resolution 3.50 Å
6V22 Cryo-EM structure of Ca2+-bound hsSlo1-beta4 channel complex Deposited 2019-11-22 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 66–1121（1056 aa） Fragment：UNP residues 66-1121
Chain B 66–1121（1056 aa） Fragment：UNP residues 66-1121
Chain C 66–1121（1056 aa） Fragment：UNP residues 66-1121
Chain D 66–1121（1056 aa） Fragment：UNP residues 66-1121
Not recorded MG MAGNESIUM ION × 4 CA CALCIUM ION × 8 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 60 CLR CHOLESTEROL × 16 K POTASSIUM ION × 4 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；Blot for 4 seconds before plunging.
Resolution 3.20 Å
6V35 Cryo-EM structure of Ca2+-free hsSlo1-beta4 channel complex Deposited 2019-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 66–1121（1056 aa）
Chain B 66–1121（1056 aa）
Chain C 66–1121（1056 aa）
Chain D 66–1121（1056 aa）
Not recorded PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 40 CLR CHOLESTEROL × 8 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；Blot for 4 seconds before plunging.
Resolution 3.50 Å
6V38 Cryo-EM structure of Ca2+-bound hsSlo1 channel Deposited 2019-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 66–1121（1056 aa）
Chain B 66–1121（1056 aa）
Chain C 66–1121（1056 aa）
Chain D 66–1121（1056 aa）
Not recorded MG MAGNESIUM ION × 4 CA CALCIUM ION × 8 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 32 CLR CHOLESTEROL × 12 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；Blot for 4 seconds before plunging.
Resolution 3.80 Å
6V3G Cryo-EM structure of Ca2+-free hsSlo1 channel Deposited 2019-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 66–1121（1056 aa）
Chain B 66–1121（1056 aa）
Chain C 66–1121（1056 aa）
Chain D 66–1121（1056 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；Blot for 4 seconds before plunging.
Resolution 4.00 Å
6V5A Crystal structure of the human BK channel gating ring L390P mutant Deposited 2019-12-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 406–1121（716 aa） Fragment：Gating Ring
Mutation：L390P CA CALCIUM ION × 1 SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.9；295 K；50 mM sodium acetate, 4% (w/v) PEG 4000, 100 mM sodium sulfate, and 100 mM lithium sulfate (pH 4.9)
Resolution 2.00 Å R-free 0.226
8GH9 Cryo-EM structure of hSlo1 in total membrane vesicles Deposited 2023-03-09 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 67–1121（1055 aa）
Chain B 67–1121（1055 aa）
Chain C 67–1121（1055 aa）
Chain D 67–1121（1055 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
8GHF cryo-EM structure of hSlo1 in plasma membrane vesicles Deposited 2023-03-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 67–1121（1055 aa）
Chain B 67–1121（1055 aa）
Chain C 67–1121（1055 aa）
Chain D 67–1121（1055 aa）
Not recorded MG MAGNESIUM ION × 4 CA CALCIUM ION × 8 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 88 CLR CHOLESTEROL × 24 NA SODIUM ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
8GHG Cryo-EM structure of hSlo1 in digitonin, Ca2+-free and EDTA-free Deposited 2023-03-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 67–1121（1055 aa）
Chain B 67–1121（1055 aa）
Chain C 67–1121（1055 aa）
Chain D 67–1121（1055 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8V60 F315A mutant of human Slo1 in presence of EDTA - resting VSD Deposited 2023-12-01 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 66–1121（1056 aa）
Chain B 66–1121（1056 aa）
Chain C 66–1121（1056 aa）
Chain D 66–1121（1056 aa）
Mutation：F315A Mutation：F315A Mutation：F315A Mutation：F315A K POTASSIUM ION × 9 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 28 CLR CHOLESTEROL × 4 AJP Digitonin × 36 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.43 Å
8V63 R207A mutant of human Slo1 in presence of EDTA - activated VSD Deposited 2023-12-01 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 66–1121（1056 aa）
Chain B 66–1121（1056 aa）
Chain C 66–1121（1056 aa）
Chain D 66–1121（1056 aa）
Mutation：R207A Mutation：R207A Mutation：R207A Mutation：R207A K POTASSIUM ION × 9 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 28 CLR CHOLESTEROL × 4 AJP Digitonin × 40 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.72 Å
8V64 RCK1-RCK2 double mutant of human Slo1 in presence of EDTA - resting VSD Deposited 2023-12-01 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 66–1121（1056 aa）
Chain B 66–1121（1056 aa）
Chain C 66–1121（1056 aa）
Chain D 66–1121（1056 aa）
Mutation：D894A,D895A,D896A,D897A Mutation：D894A,D895A,D896A,D897A Mutation：D894A,D895A,D896A,D897A Mutation：D894A,D895A,D896A,D897A K POTASSIUM ION × 9 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 24 CLR CHOLESTEROL × 4 AJP Digitonin × 36 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å
8VAV Human Slo1 - human LRRC26 in presence of EDTA - GR masked Deposited 2023-12-11 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 66–1121（1056 aa）
Chain B 66–1121（1056 aa）
Chain C 66–1121（1056 aa）
Chain D 66–1121（1056 aa）
Not recorded POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 20 K POTASSIUM ION × 4 AJP Digitonin × 32 CLR CHOLESTEROL × 4 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.13 Å
8VAZ Structure of human Slo1 and human LRRC26 in EDTA - LRRD masked Deposited 2023-12-11 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 66–1121（1056 aa）
Chain B 66–1121（1056 aa）
Chain C 66–1121（1056 aa）
Chain D 66–1121（1056 aa）
Not recorded K POTASSIUM ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.82 Å
8Z3S Activation mechanism and novel binding site of the BKCa channel activator CTIBD Deposited 2024-04-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 66–1179（1114 aa）
Chain B 66–1179（1114 aa）
Chain C 66–1179（1114 aa）
Chain D 66–1179（1114 aa）
Not recorded A1L04 4-[4-(4-chlorophenyl)-3-(trifluoromethyl)-1,2-oxazol-5-yl]benzene-1,3-diol × 8 Y01 CHOLESTEROL HEMISUCCINATE × 4 MG MAGNESIUM ION × 4 CA CALCIUM ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
9CZH Ca2+ bound intermediate state of hSlo1 + beta2N-beta4 channel in detergent. Deposited 2024-08-05 Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric
Chain A 66–1121（1056 aa）
Chain B 66–1121（1056 aa）
Chain C 66–1121（1056 aa）
Chain D 66–1121（1056 aa）
Not recorded CA CALCIUM ION × 8 K POTASSIUM ION × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 10 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20 mM Tris-HCl pH 8.0, 450 mM KCl, 5 mM EDTA, 15 mM MgCl2, 0.02% GDN and 0.05 mg/ml POPE:POPC:POPA 5:5:1 (w:w:w).
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9CZJ Ca2+ free hSlo1 + beta2N-beta4 channel in detergent. Deposited 2024-08-05 Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric
Chain A 66–1121（1056 aa）
Chain B 66–1121（1056 aa）
Chain C 66–1121（1056 aa）
Chain D 66–1121（1056 aa）
Not recorded CLR CHOLESTEROL × 4 K POTASSIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20 mM Tris-HCl pH 8.0, 450 mM KCl, 5 mM EDTA, 15 mM MgCl2, 0.02% GDN and 0.05 mg/ml POPE:POPC:POPA 5:5:1 (w:w:w).
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.54 Å
9CZK Ca2+ free hSlo1 + beta2N-beta4 channel in nanodisc. Deposited 2024-08-05 Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric
Chain A 66–1121（1056 aa）
Chain B 66–1121（1056 aa）
Chain C 66–1121（1056 aa）
Chain D 66–1121（1056 aa）
Not recorded K POTASSIUM ION × 2 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 4 CLR CHOLESTEROL × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20 mM Tris-HCl pH 8.0, 450 mM KCl, 5 mM EDTA, 15 mM MgCl2, 0.02% GDN and 0.05 mg/ml POPE:POPC:POPA 5:5:1 (w:w:w).
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
9CZM Ca2+ bound open-inactivated hSlo1 + beta2N-beta4 channel in nanodisc. Deposited 2024-08-05 Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric
Chain A 66–1121（1056 aa）
Chain B 66–1121（1056 aa）
Chain C 66–1121（1056 aa）
Chain D 66–1121（1056 aa）
Not recorded POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 32 MG MAGNESIUM ION × 4 CA CALCIUM ION × 8 CLR CHOLESTEROL × 8 K POTASSIUM ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20 mM Tris-HCl pH 8.0, 450 mM KCl, 5 mM EDTA, 15 mM MgCl2, 0.02% GDN and 0.05 mg/ml POPE:POPC:POPA 5:5:1 (w:w:w).
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.57 Å
9CZO Ca2+ bound intermediate state of hSlo1 + beta2N-beta4 channel in nanodisc. Deposited 2024-08-05 Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric
Chain A 66–1121（1056 aa）
Chain B 66–1121（1056 aa）
Chain C 66–1121（1056 aa）
Chain D 66–1121（1056 aa）
Not recorded CA CALCIUM ION × 8 K POTASSIUM ION × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 11 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20 mM Tris-HCl pH 8.0, 450 mM KCl, 5 mM EDTA, 15 mM MgCl2, 0.02% GDN and 0.05 mg/ml POPE:POPC:POPA 5:5:1 (w:w:w).
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.87 Å
9D18 Ca2+ bound open-inactivated hSlo1 + beta2N-beta4 channel in detergent-conformation 2 of inactivating domain Deposited 2024-08-07 Parsed fields agree Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric
Chain A 66–1121（1056 aa）
Chain B 66–1121（1056 aa）
Chain C 66–1121（1056 aa）
Chain D 66–1121（1056 aa）
Not recorded MG MAGNESIUM ION × 4 CA CALCIUM ION × 8 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 20 CLR CHOLESTEROL × 11 K POTASSIUM ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20 mM Tris-HCl pH 8.0, 450 mM KCl, 5 mM EDTA, 15 mM MgCl2, 0.02% GDN and 0.05 mg/ml POPE:POPC:POPA 5:5:1 (w:w:w).
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.88 Å
9D19 Ca2+ bound open-inactivated hSlo1 + beta2N-beta4 channel in detergent-conformation 3 of inactivating domain Deposited 2024-08-07 Parsed fields agree Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric
Chain A 66–1121（1056 aa）
Chain B 66–1121（1056 aa）
Chain C 66–1121（1056 aa）
Chain D 66–1121（1056 aa）
Not recorded MG MAGNESIUM ION × 4 CA CALCIUM ION × 8 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 20 CLR CHOLESTEROL × 11 K POTASSIUM ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20 mM Tris-HCl pH 8.0, 450 mM KCl, 5 mM EDTA, 15 mM MgCl2, 0.02% GDN and 0.05 mg/ml POPE:POPC:POPA 5:5:1 (w:w:w).
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.88 Å
9JO3 Cryo-EM structure of human BKca channel-compound 10b complex Deposited 2024-09-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 66–1184（1119 aa）
Chain B 66–1184（1119 aa）
Chain C 66–1184（1119 aa）
Chain D 66–1184（1119 aa）
Not recorded MG MAGNESIUM ION × 4 CA CALCIUM ION × 8 K POTASSIUM ION × 3 A1L4E 5-azanyl-2-[2,3,5,6-tetrakis(fluoranyl)-4-(trifluoromethyl)phenoxy]phenol × 4 Y01 CHOLESTEROL HEMISUCCINATE × 16 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
9JO4 Cryo-EM structure of human BKca channel-compound 51b complex Deposited 2024-09-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 66–1179（1114 aa）
Chain B 66–1179（1114 aa）
Chain C 66–1179（1114 aa）
Chain D 66–1179（1114 aa）
Not recorded A1L4F 5-(5-morpholin-4-ylpentylamino)-2-[2,3,5,6-tetrakis(fluoranyl)-4-(trifluoromethyl)phenoxy]phenol × 4 MG MAGNESIUM ION × 4 CA CALCIUM ION × 8 K POTASSIUM ION × 3 Y01 CHOLESTEROL HEMISUCCINATE × 16 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å

27 other PDB entries and 27 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name KCMA1_HUMAN
Isoform Q12791-5
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 1–1056； UniProt 66–1121 Author chain B； PDBConstruct 1–1056； UniProt 66–1121 Author chain C； PDBConstruct 1–1056； UniProt 66–1121 Author chain D； PDBConstruct 1–1056； UniProt 66–1121

Large-conductance Ca2+-activated K+ channel beta2 subunit,Calcium-activated potassium channel subunit beta-4

Homo sapiens

UniProt B5BNX0

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Insufficient information Heteromer Protein × 8 PDB declaration: octameric（8） Consistent with protein copy count Chain E； UniProt 2–44 Chain F； UniProt 2–44 Chain G； UniProt 2–44 Chain H； UniProt 2–44 Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ; Isoform 5 of Calcium-activated potassium channel subunit alpha-1 × 4 （Q12791） MG MAGNESIUM ION × 4 CA CALCIUM ION × 8 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 20 CLR CHOLESTEROL × 11 K POTASSIUM ION × 4 ELECTRON MICROSCOPY cryo-EM buffer：pH 8；20 mM Tris-HCl pH 8.0, 450 mM KCl, 5 mM EDTA, 15 mM MgCl2, 0.02% GDN and 0.05 mg/ml POPE:POPC:POPA 5:5:1 (w:w:w). cryo-EM vitrification conditions：Cryogen ETHANE Resolution 2.88 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9CZQ Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
9CZH Ca2+ bound intermediate state of hSlo1 + beta2N-beta4 channel in detergent. Deposited 2024-08-05 Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric
Chain E 2–44（43 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ;
Chain F 2–44（43 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ;
Chain G 2–44（43 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ;
Chain H 2–44（43 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ;
Not recorded CA CALCIUM ION × 8 K POTASSIUM ION × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 10 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20 mM Tris-HCl pH 8.0, 450 mM KCl, 5 mM EDTA, 15 mM MgCl2, 0.02% GDN and 0.05 mg/ml POPE:POPC:POPA 5:5:1 (w:w:w).
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9CZJ Ca2+ free hSlo1 + beta2N-beta4 channel in detergent. Deposited 2024-08-05 Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric
Chain E 2–44（43 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ;
Chain F 2–44（43 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ;
Chain G 2–44（43 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ;
Chain H 2–44（43 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ;
Not recorded CLR CHOLESTEROL × 4 K POTASSIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20 mM Tris-HCl pH 8.0, 450 mM KCl, 5 mM EDTA, 15 mM MgCl2, 0.02% GDN and 0.05 mg/ml POPE:POPC:POPA 5:5:1 (w:w:w).
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.54 Å
9CZK Ca2+ free hSlo1 + beta2N-beta4 channel in nanodisc. Deposited 2024-08-05 Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric
Chain E 2–44（43 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ;
Chain F 2–44（43 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ;
Chain G 2–44（43 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ;
Chain H 2–44（43 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ;
Not recorded K POTASSIUM ION × 2 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 4 CLR CHOLESTEROL × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20 mM Tris-HCl pH 8.0, 450 mM KCl, 5 mM EDTA, 15 mM MgCl2, 0.02% GDN and 0.05 mg/ml POPE:POPC:POPA 5:5:1 (w:w:w).
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
9CZM Ca2+ bound open-inactivated hSlo1 + beta2N-beta4 channel in nanodisc. Deposited 2024-08-05 Different construct Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric
Chain E 2–44（43 aa）
Chain F 2–44（43 aa）
Chain G 2–44（43 aa）
Chain H 2–44（43 aa）
Not recorded POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 32 MG MAGNESIUM ION × 4 CA CALCIUM ION × 8 CLR CHOLESTEROL × 8 K POTASSIUM ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20 mM Tris-HCl pH 8.0, 450 mM KCl, 5 mM EDTA, 15 mM MgCl2, 0.02% GDN and 0.05 mg/ml POPE:POPC:POPA 5:5:1 (w:w:w).
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.57 Å
9CZO Ca2+ bound intermediate state of hSlo1 + beta2N-beta4 channel in nanodisc. Deposited 2024-08-05 Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric
Chain E 2–44（43 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ;
Chain F 2–44（43 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ;
Chain G 2–44（43 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ;
Chain H 2–44（43 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ;
Not recorded CA CALCIUM ION × 8 K POTASSIUM ION × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 11 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20 mM Tris-HCl pH 8.0, 450 mM KCl, 5 mM EDTA, 15 mM MgCl2, 0.02% GDN and 0.05 mg/ml POPE:POPC:POPA 5:5:1 (w:w:w).
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.87 Å
9D18 Ca2+ bound open-inactivated hSlo1 + beta2N-beta4 channel in detergent-conformation 2 of inactivating domain Deposited 2024-08-07 Parsed fields agree Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric
Chain E 2–44（43 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ;
Chain F 2–44（43 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ;
Chain G 2–44（43 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ;
Chain H 2–44（43 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ;
Not recorded MG MAGNESIUM ION × 4 CA CALCIUM ION × 8 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 20 CLR CHOLESTEROL × 11 K POTASSIUM ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20 mM Tris-HCl pH 8.0, 450 mM KCl, 5 mM EDTA, 15 mM MgCl2, 0.02% GDN and 0.05 mg/ml POPE:POPC:POPA 5:5:1 (w:w:w).
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.88 Å
9D19 Ca2+ bound open-inactivated hSlo1 + beta2N-beta4 channel in detergent-conformation 3 of inactivating domain Deposited 2024-08-07 Parsed fields agree Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric
Chain E 2–44（43 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ;
Chain F 2–44（43 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ;
Chain G 2–44（43 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ;
Chain H 2–44（43 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ;
Not recorded MG MAGNESIUM ION × 4 CA CALCIUM ION × 8 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 20 CLR CHOLESTEROL × 11 K POTASSIUM ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20 mM Tris-HCl pH 8.0, 450 mM KCl, 5 mM EDTA, 15 mM MgCl2, 0.02% GDN and 0.05 mg/ml POPE:POPC:POPA 5:5:1 (w:w:w).
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.88 Å

7 other PDB entries and 7 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name B5BNX0_HUMAN
Isoform —
PDB entities 2
Chains and sequence ranges Author chain E； PDBConstruct 1–43； UniProt 2–44 Author chain F； PDBConstruct 1–43； UniProt 2–44 Author chain G； PDBConstruct 1–43； UniProt 2–44 Author chain H； PDBConstruct 1–43； UniProt 2–44

Large-conductance Ca2+-activated K+ channel beta2 subunit,Calcium-activated potassium channel subunit beta-4

Homo sapiens

UniProt Q86W47

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Insufficient information Heteromer Protein × 8 PDB declaration: octameric（8） Consistent with protein copy count Chain E； UniProt 15–210 Chain F； UniProt 15–210 Chain G； UniProt 15–210 Chain H； UniProt 15–210 Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ; Isoform 5 of Calcium-activated potassium channel subunit alpha-1 × 4 （Q12791） MG MAGNESIUM ION × 4 CA CALCIUM ION × 8 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 20 CLR CHOLESTEROL × 11 K POTASSIUM ION × 4 ELECTRON MICROSCOPY cryo-EM buffer：pH 8；20 mM Tris-HCl pH 8.0, 450 mM KCl, 5 mM EDTA, 15 mM MgCl2, 0.02% GDN and 0.05 mg/ml POPE:POPC:POPA 5:5:1 (w:w:w). cryo-EM vitrification conditions：Cryogen ETHANE Resolution 2.88 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9CZQ Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5Y7L Solution structure of Hbeta4 extracellular loop of BK potassium channel Deposited 2017-08-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 45–166（122 aa） Fragment：UNP RESIDUES 45-166
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 6.8；293 K；Ionic strength (raw mmCIF value) 75；Pressure 1
NMR sample composition 0.5 mM [U-13C; U-15N] Hbeta4, 25 mM sodium phosphate, 50 mM sodium chloride, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 0.5 mM [U-13C; U-15N] Hbeta4, 25 mM sodium phosphate, 50 mM sodium chloride, 100% D2O | 100% D2O
Resolution not provided
6V22 Cryo-EM structure of Ca2+-bound hsSlo1-beta4 channel complex Deposited 2019-11-22 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain E 1–210（210 aa）
Chain F 1–210（210 aa）
Chain G 1–210（210 aa）
Chain H 1–210（210 aa）
Not recorded MG MAGNESIUM ION × 4 CA CALCIUM ION × 8 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 60 CLR CHOLESTEROL × 16 K POTASSIUM ION × 4 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；Blot for 4 seconds before plunging.
Resolution 3.20 Å
6V35 Cryo-EM structure of Ca2+-free hsSlo1-beta4 channel complex Deposited 2019-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain E 1–210（210 aa）
Chain F 1–210（210 aa）
Chain G 1–210（210 aa）
Chain H 1–210（210 aa）
Not recorded PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 40 CLR CHOLESTEROL × 8 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；Blot for 4 seconds before plunging.
Resolution 3.50 Å
9CZH Ca2+ bound intermediate state of hSlo1 + beta2N-beta4 channel in detergent. Deposited 2024-08-05 Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric
Chain E 15–210（196 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ;
Chain F 15–210（196 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ;
Chain G 15–210（196 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ;
Chain H 15–210（196 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ;
Not recorded CA CALCIUM ION × 8 K POTASSIUM ION × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 10 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20 mM Tris-HCl pH 8.0, 450 mM KCl, 5 mM EDTA, 15 mM MgCl2, 0.02% GDN and 0.05 mg/ml POPE:POPC:POPA 5:5:1 (w:w:w).
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9CZJ Ca2+ free hSlo1 + beta2N-beta4 channel in detergent. Deposited 2024-08-05 Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric
Chain E 15–210（196 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ;
Chain F 15–210（196 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ;
Chain G 15–210（196 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ;
Chain H 15–210（196 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ;
Not recorded CLR CHOLESTEROL × 4 K POTASSIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20 mM Tris-HCl pH 8.0, 450 mM KCl, 5 mM EDTA, 15 mM MgCl2, 0.02% GDN and 0.05 mg/ml POPE:POPC:POPA 5:5:1 (w:w:w).
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.54 Å
9CZK Ca2+ free hSlo1 + beta2N-beta4 channel in nanodisc. Deposited 2024-08-05 Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric
Chain E 15–210（196 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ;
Chain F 15–210（196 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ;
Chain G 15–210（196 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ;
Chain H 15–210（196 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ;
Not recorded K POTASSIUM ION × 2 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 4 CLR CHOLESTEROL × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20 mM Tris-HCl pH 8.0, 450 mM KCl, 5 mM EDTA, 15 mM MgCl2, 0.02% GDN and 0.05 mg/ml POPE:POPC:POPA 5:5:1 (w:w:w).
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
9CZM Ca2+ bound open-inactivated hSlo1 + beta2N-beta4 channel in nanodisc. Deposited 2024-08-05 Different construct Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric
Chain E 15–210（196 aa）
Chain F 15–210（196 aa）
Chain G 15–210（196 aa）
Chain H 15–210（196 aa）
Not recorded POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 32 MG MAGNESIUM ION × 4 CA CALCIUM ION × 8 CLR CHOLESTEROL × 8 K POTASSIUM ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20 mM Tris-HCl pH 8.0, 450 mM KCl, 5 mM EDTA, 15 mM MgCl2, 0.02% GDN and 0.05 mg/ml POPE:POPC:POPA 5:5:1 (w:w:w).
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.57 Å
9CZO Ca2+ bound intermediate state of hSlo1 + beta2N-beta4 channel in nanodisc. Deposited 2024-08-05 Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric
Chain E 15–210（196 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ;
Chain F 15–210（196 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ;
Chain G 15–210（196 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ;
Chain H 15–210（196 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ;
Not recorded CA CALCIUM ION × 8 K POTASSIUM ION × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 11 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20 mM Tris-HCl pH 8.0, 450 mM KCl, 5 mM EDTA, 15 mM MgCl2, 0.02% GDN and 0.05 mg/ml POPE:POPC:POPA 5:5:1 (w:w:w).
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.87 Å
9D18 Ca2+ bound open-inactivated hSlo1 + beta2N-beta4 channel in detergent-conformation 2 of inactivating domain Deposited 2024-08-07 Parsed fields agree Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric
Chain E 15–210（196 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ;
Chain F 15–210（196 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ;
Chain G 15–210（196 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ;
Chain H 15–210（196 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ;
Not recorded MG MAGNESIUM ION × 4 CA CALCIUM ION × 8 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 20 CLR CHOLESTEROL × 11 K POTASSIUM ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20 mM Tris-HCl pH 8.0, 450 mM KCl, 5 mM EDTA, 15 mM MgCl2, 0.02% GDN and 0.05 mg/ml POPE:POPC:POPA 5:5:1 (w:w:w).
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.88 Å
9D19 Ca2+ bound open-inactivated hSlo1 + beta2N-beta4 channel in detergent-conformation 3 of inactivating domain Deposited 2024-08-07 Parsed fields agree Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric
Chain E 15–210（196 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ;
Chain F 15–210（196 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ;
Chain G 15–210（196 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ;
Chain H 15–210（196 aa） Fragment：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ;
Not recorded MG MAGNESIUM ION × 4 CA CALCIUM ION × 8 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 20 CLR CHOLESTEROL × 11 K POTASSIUM ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20 mM Tris-HCl pH 8.0, 450 mM KCl, 5 mM EDTA, 15 mM MgCl2, 0.02% GDN and 0.05 mg/ml POPE:POPC:POPA 5:5:1 (w:w:w).
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.88 Å

10 other PDB entries and 10 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name KCMB4_HUMAN
Isoform —
PDB entities 2
Chains and sequence ranges Author chain E； PDBConstruct 44–239； UniProt 15–210 Author chain F； PDBConstruct 44–239； UniProt 15–210 Author chain G； PDBConstruct 44–239； UniProt 15–210 Author chain H； PDBConstruct 44–239； UniProt 15–210

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 9czq

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 9czq
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2. Structure Basics 2. Structure Basics

Entry ID entry_id9czq
Deposition date deposition_date2024-08-05
Structure title titleCa2+ bound open-inactivated hSlo1 + beta2N-beta4 channel in detergent.
Keywords keywords;Potassium ion channel, calcium and voltage gated ion channel, big potassium channel, human BK, hSlo1, open-inactivated hSlo1, ball and chain inactivation, hSlo1 inactivating subunit complex, detergent micelle, MEMBRANE PROTEIN ;; MEMBRANE PROTEIN
Experimental Method methodELECTRON MICROSCOPY
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier55.07 Å
Radius of gyration Rg (electron density) rg_electron53.87 Å
Forward intensity I(0) i03260700000.00
Molecular weight molecular_weight512410.0 kDa
Excluded volume excluded_volume653860 ų
Envelope volume envelope_volume942920 ų
Hydration-shell volume shell_volume139130 ų
Envelope diameter envelope_diameter168.9 Å
Shell Rg shell_rg62.69 Å
Envelope Rg envelope_rg52.28 Å
Shape Rg shape_rg53.87 Å
Total Rg total_rg54.11 Å
Total atoms total_atoms36018
Residues n_residues4392
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax172.2 Å
Rg (real space) rg_real54.77 Å
Rg uncertainty (real space) rg_real_error1.43 Å
I(0) (real space) i0_real3.2610e+09
I(0) uncertainty (real space) i0_real_error6.9330e+07
Rg (reciprocal space) rg_reciprocal55.31 Å
I(0) (reciprocal space) i0_reciprocal3263000000.0000
Solution quality estimate total_estimate0.8832
Solution quality rating solution_quality GOOD a GOOD solution
P(r) peaks n_peaks2
Primary peak position r_peak_primary71.2 Å
Skewness Skewness skewness0.091
Kurtosis Kurtosis kurtosis-0.534
Angular range angular_range— – 0.1450 Å−1
Current regularization parameter α current_alpha0.0000
Highest regularization parameter α highest_alpha476900000.0000
Real-space data points n_real_points30
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.914; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.953; Smooth: 0.783
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (7)

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8. Citations (1)

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9. Files and Curves (10)