9ei4

Cryo-EM structure of Human RNA polymerase II Elongation Complex bound to the RECQL5 helicase in the presence of ADP

Method: ELECTRON MICROSCOPY

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1. Protein Identity and Related Structures Protein Identity & Related Structures

DNA-directed RNA polymerase II subunit RPB1

OrganismNot specified

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 13 DNA 2 RNA 1	DNA-directed RNA polymerase II subunit RPB2 × 1 （P30876） DNA-directed RNA polymerase II subunit RPB3 × 1 （P19387） DNA-directed RNA polymerase II subunit RPB4 × 1 （O15514） DNA-directed RNA polymerases I, II, and III subunit RPABC1 × 1 （P19388） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （P61218） DNA-directed RNA polymerase II subunit RPB7 × 1 （P62487） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （P52434） DNA-directed RNA polymerase II subunit RPB9 × 1 （P36954） DNA-directed RNA polymerases I, II, and III subunit RPABC5 × 1 （P62875） DNA-directed RNA polymerase II subunit RPB11-a × 1 （P52435） DNA-directed RNA polymerases I, II, and III subunit RPABC4 × 1 （P53803） Non-template DNA, nucleic acid scaffold × 1 RNA, nucleic acid scaffold × 1 Template DNA, nucleic acid scaffold × 1 ATP-dependent DNA helicase Q5 × 1 （O94762） ZINC ION × 8 MAGNESIUM ION × 1 ADENOSINE-5'-DIPHOSPHATE × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPB1_HUMAN
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 1–1970； UniProt 1–1970

DNA-directed RNA polymerase II subunit RPB2

OrganismNot specified

UniProt P30876

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 13 DNA 2 RNA 1	DNA-directed RNA polymerase II subunit RPB1 × 1 （P24928） DNA-directed RNA polymerase II subunit RPB3 × 1 （P19387） DNA-directed RNA polymerase II subunit RPB4 × 1 （O15514） DNA-directed RNA polymerases I, II, and III subunit RPABC1 × 1 （P19388） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （P61218） DNA-directed RNA polymerase II subunit RPB7 × 1 （P62487） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （P52434） DNA-directed RNA polymerase II subunit RPB9 × 1 （P36954） DNA-directed RNA polymerases I, II, and III subunit RPABC5 × 1 （P62875） DNA-directed RNA polymerase II subunit RPB11-a × 1 （P52435） DNA-directed RNA polymerases I, II, and III subunit RPABC4 × 1 （P53803） Non-template DNA, nucleic acid scaffold × 1 RNA, nucleic acid scaffold × 1 Template DNA, nucleic acid scaffold × 1 ATP-dependent DNA helicase Q5 × 1 （O94762） ZINC ION × 8 MAGNESIUM ION × 1 ADENOSINE-5'-DIPHOSPHATE × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPB2_HUMAN
Isoform	—
PDB entities	2
Chains and sequence ranges	Author chain B； PDBConstruct 1–1174； UniProt 1–1174

DNA-directed RNA polymerase II subunit RPB3

OrganismNot specified

UniProt P19387

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 13 DNA 2 RNA 1	DNA-directed RNA polymerase II subunit RPB1 × 1 （P24928） DNA-directed RNA polymerase II subunit RPB2 × 1 （P30876） DNA-directed RNA polymerase II subunit RPB4 × 1 （O15514） DNA-directed RNA polymerases I, II, and III subunit RPABC1 × 1 （P19388） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （P61218） DNA-directed RNA polymerase II subunit RPB7 × 1 （P62487） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （P52434） DNA-directed RNA polymerase II subunit RPB9 × 1 （P36954） DNA-directed RNA polymerases I, II, and III subunit RPABC5 × 1 （P62875） DNA-directed RNA polymerase II subunit RPB11-a × 1 （P52435） DNA-directed RNA polymerases I, II, and III subunit RPABC4 × 1 （P53803） Non-template DNA, nucleic acid scaffold × 1 RNA, nucleic acid scaffold × 1 Template DNA, nucleic acid scaffold × 1 ATP-dependent DNA helicase Q5 × 1 （O94762） ZINC ION × 8 MAGNESIUM ION × 1 ADENOSINE-5'-DIPHOSPHATE × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPB3_HUMAN
Isoform	—
PDB entities	3
Chains and sequence ranges	Author chain C； PDBConstruct 1–275； UniProt 1–275

DNA-directed RNA polymerase II subunit RPB4

OrganismNot specified

UniProt O15514

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 13 DNA 2 RNA 1	DNA-directed RNA polymerase II subunit RPB1 × 1 （P24928） DNA-directed RNA polymerase II subunit RPB2 × 1 （P30876） DNA-directed RNA polymerase II subunit RPB3 × 1 （P19387） DNA-directed RNA polymerases I, II, and III subunit RPABC1 × 1 （P19388） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （P61218） DNA-directed RNA polymerase II subunit RPB7 × 1 （P62487） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （P52434） DNA-directed RNA polymerase II subunit RPB9 × 1 （P36954） DNA-directed RNA polymerases I, II, and III subunit RPABC5 × 1 （P62875） DNA-directed RNA polymerase II subunit RPB11-a × 1 （P52435） DNA-directed RNA polymerases I, II, and III subunit RPABC4 × 1 （P53803） Non-template DNA, nucleic acid scaffold × 1 RNA, nucleic acid scaffold × 1 Template DNA, nucleic acid scaffold × 1 ATP-dependent DNA helicase Q5 × 1 （O94762） ZINC ION × 8 MAGNESIUM ION × 1 ADENOSINE-5'-DIPHOSPHATE × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPB4_HUMAN
Isoform	—
PDB entities	4
Chains and sequence ranges	Author chain D； PDBConstruct 1–142； UniProt 1–142

DNA-directed RNA polymerases I, II, and III subunit RPABC1

OrganismNot specified

UniProt P19388

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 13 DNA 2 RNA 1	DNA-directed RNA polymerase II subunit RPB1 × 1 （P24928） DNA-directed RNA polymerase II subunit RPB2 × 1 （P30876） DNA-directed RNA polymerase II subunit RPB3 × 1 （P19387） DNA-directed RNA polymerase II subunit RPB4 × 1 （O15514） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （P61218） DNA-directed RNA polymerase II subunit RPB7 × 1 （P62487） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （P52434） DNA-directed RNA polymerase II subunit RPB9 × 1 （P36954） DNA-directed RNA polymerases I, II, and III subunit RPABC5 × 1 （P62875） DNA-directed RNA polymerase II subunit RPB11-a × 1 （P52435） DNA-directed RNA polymerases I, II, and III subunit RPABC4 × 1 （P53803） Non-template DNA, nucleic acid scaffold × 1 RNA, nucleic acid scaffold × 1 Template DNA, nucleic acid scaffold × 1 ATP-dependent DNA helicase Q5 × 1 （O94762） ZINC ION × 8 MAGNESIUM ION × 1 ADENOSINE-5'-DIPHOSPHATE × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPAB1_HUMAN
Isoform	—
PDB entities	5
Chains and sequence ranges	Author chain E； PDBConstruct 1–210； UniProt 1–210

DNA-directed RNA polymerases I, II, and III subunit RPABC2

OrganismNot specified

UniProt P61218

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 13 DNA 2 RNA 1	DNA-directed RNA polymerase II subunit RPB1 × 1 （P24928） DNA-directed RNA polymerase II subunit RPB2 × 1 （P30876） DNA-directed RNA polymerase II subunit RPB3 × 1 （P19387） DNA-directed RNA polymerase II subunit RPB4 × 1 （O15514） DNA-directed RNA polymerases I, II, and III subunit RPABC1 × 1 （P19388） DNA-directed RNA polymerase II subunit RPB7 × 1 （P62487） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （P52434） DNA-directed RNA polymerase II subunit RPB9 × 1 （P36954） DNA-directed RNA polymerases I, II, and III subunit RPABC5 × 1 （P62875） DNA-directed RNA polymerase II subunit RPB11-a × 1 （P52435） DNA-directed RNA polymerases I, II, and III subunit RPABC4 × 1 （P53803） Non-template DNA, nucleic acid scaffold × 1 RNA, nucleic acid scaffold × 1 Template DNA, nucleic acid scaffold × 1 ATP-dependent DNA helicase Q5 × 1 （O94762） ZINC ION × 8 MAGNESIUM ION × 1 ADENOSINE-5'-DIPHOSPHATE × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPAB2_HUMAN
Isoform	—
PDB entities	6
Chains and sequence ranges	Author chain F； PDBConstruct 1–127； UniProt 1–127

DNA-directed RNA polymerase II subunit RPB7

OrganismNot specified

UniProt P62487

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 13 DNA 2 RNA 1	DNA-directed RNA polymerase II subunit RPB1 × 1 （P24928） DNA-directed RNA polymerase II subunit RPB2 × 1 （P30876） DNA-directed RNA polymerase II subunit RPB3 × 1 （P19387） DNA-directed RNA polymerase II subunit RPB4 × 1 （O15514） DNA-directed RNA polymerases I, II, and III subunit RPABC1 × 1 （P19388） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （P61218） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （P52434） DNA-directed RNA polymerase II subunit RPB9 × 1 （P36954） DNA-directed RNA polymerases I, II, and III subunit RPABC5 × 1 （P62875） DNA-directed RNA polymerase II subunit RPB11-a × 1 （P52435） DNA-directed RNA polymerases I, II, and III subunit RPABC4 × 1 （P53803） Non-template DNA, nucleic acid scaffold × 1 RNA, nucleic acid scaffold × 1 Template DNA, nucleic acid scaffold × 1 ATP-dependent DNA helicase Q5 × 1 （O94762） ZINC ION × 8 MAGNESIUM ION × 1 ADENOSINE-5'-DIPHOSPHATE × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPB7_HUMAN
Isoform	—
PDB entities	7
Chains and sequence ranges	Author chain G； PDBConstruct 1–172； UniProt 1–172

DNA-directed RNA polymerases I, II, and III subunit RPABC3

OrganismNot specified

UniProt P52434

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 13 DNA 2 RNA 1	DNA-directed RNA polymerase II subunit RPB1 × 1 （P24928） DNA-directed RNA polymerase II subunit RPB2 × 1 （P30876） DNA-directed RNA polymerase II subunit RPB3 × 1 （P19387） DNA-directed RNA polymerase II subunit RPB4 × 1 （O15514） DNA-directed RNA polymerases I, II, and III subunit RPABC1 × 1 （P19388） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （P61218） DNA-directed RNA polymerase II subunit RPB7 × 1 （P62487） DNA-directed RNA polymerase II subunit RPB9 × 1 （P36954） DNA-directed RNA polymerases I, II, and III subunit RPABC5 × 1 （P62875） DNA-directed RNA polymerase II subunit RPB11-a × 1 （P52435） DNA-directed RNA polymerases I, II, and III subunit RPABC4 × 1 （P53803） Non-template DNA, nucleic acid scaffold × 1 RNA, nucleic acid scaffold × 1 Template DNA, nucleic acid scaffold × 1 ATP-dependent DNA helicase Q5 × 1 （O94762） ZINC ION × 8 MAGNESIUM ION × 1 ADENOSINE-5'-DIPHOSPHATE × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPAB3_HUMAN
Isoform	—
PDB entities	8
Chains and sequence ranges	Author chain H； PDBConstruct 1–150； UniProt 1–150

DNA-directed RNA polymerase II subunit RPB9

OrganismNot specified

UniProt P36954

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 13 DNA 2 RNA 1	DNA-directed RNA polymerase II subunit RPB1 × 1 （P24928） DNA-directed RNA polymerase II subunit RPB2 × 1 （P30876） DNA-directed RNA polymerase II subunit RPB3 × 1 （P19387） DNA-directed RNA polymerase II subunit RPB4 × 1 （O15514） DNA-directed RNA polymerases I, II, and III subunit RPABC1 × 1 （P19388） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （P61218） DNA-directed RNA polymerase II subunit RPB7 × 1 （P62487） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （P52434） DNA-directed RNA polymerases I, II, and III subunit RPABC5 × 1 （P62875） DNA-directed RNA polymerase II subunit RPB11-a × 1 （P52435） DNA-directed RNA polymerases I, II, and III subunit RPABC4 × 1 （P53803） Non-template DNA, nucleic acid scaffold × 1 RNA, nucleic acid scaffold × 1 Template DNA, nucleic acid scaffold × 1 ATP-dependent DNA helicase Q5 × 1 （O94762） ZINC ION × 8 MAGNESIUM ION × 1 ADENOSINE-5'-DIPHOSPHATE × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPB9_HUMAN
Isoform	—
PDB entities	9
Chains and sequence ranges	Author chain I； PDBConstruct 1–125； UniProt 1–125

DNA-directed RNA polymerases I, II, and III subunit RPABC5

OrganismNot specified

UniProt P62875

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 13 DNA 2 RNA 1	DNA-directed RNA polymerase II subunit RPB1 × 1 （P24928） DNA-directed RNA polymerase II subunit RPB2 × 1 （P30876） DNA-directed RNA polymerase II subunit RPB3 × 1 （P19387） DNA-directed RNA polymerase II subunit RPB4 × 1 （O15514） DNA-directed RNA polymerases I, II, and III subunit RPABC1 × 1 （P19388） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （P61218） DNA-directed RNA polymerase II subunit RPB7 × 1 （P62487） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （P52434） DNA-directed RNA polymerase II subunit RPB9 × 1 （P36954） DNA-directed RNA polymerase II subunit RPB11-a × 1 （P52435） DNA-directed RNA polymerases I, II, and III subunit RPABC4 × 1 （P53803） Non-template DNA, nucleic acid scaffold × 1 RNA, nucleic acid scaffold × 1 Template DNA, nucleic acid scaffold × 1 ATP-dependent DNA helicase Q5 × 1 （O94762） ZINC ION × 8 MAGNESIUM ION × 1 ADENOSINE-5'-DIPHOSPHATE × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPAB5_HUMAN
Isoform	—
PDB entities	10
Chains and sequence ranges	Author chain J； PDBConstruct 1–67； UniProt 1–67

DNA-directed RNA polymerase II subunit RPB11-a

OrganismNot specified

UniProt P52435

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 13 DNA 2 RNA 1	DNA-directed RNA polymerase II subunit RPB1 × 1 （P24928） DNA-directed RNA polymerase II subunit RPB2 × 1 （P30876） DNA-directed RNA polymerase II subunit RPB3 × 1 （P19387） DNA-directed RNA polymerase II subunit RPB4 × 1 （O15514） DNA-directed RNA polymerases I, II, and III subunit RPABC1 × 1 （P19388） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （P61218） DNA-directed RNA polymerase II subunit RPB7 × 1 （P62487） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （P52434） DNA-directed RNA polymerase II subunit RPB9 × 1 （P36954） DNA-directed RNA polymerases I, II, and III subunit RPABC5 × 1 （P62875） DNA-directed RNA polymerases I, II, and III subunit RPABC4 × 1 （P53803） Non-template DNA, nucleic acid scaffold × 1 RNA, nucleic acid scaffold × 1 Template DNA, nucleic acid scaffold × 1 ATP-dependent DNA helicase Q5 × 1 （O94762） ZINC ION × 8 MAGNESIUM ION × 1 ADENOSINE-5'-DIPHOSPHATE × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPB11_HUMAN
Isoform	—
PDB entities	11
Chains and sequence ranges	Author chain K； PDBConstruct 1–117； UniProt 1–117

DNA-directed RNA polymerases I, II, and III subunit RPABC4

OrganismNot specified

UniProt P53803

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 13 DNA 2 RNA 1	DNA-directed RNA polymerase II subunit RPB1 × 1 （P24928） DNA-directed RNA polymerase II subunit RPB2 × 1 （P30876） DNA-directed RNA polymerase II subunit RPB3 × 1 （P19387） DNA-directed RNA polymerase II subunit RPB4 × 1 （O15514） DNA-directed RNA polymerases I, II, and III subunit RPABC1 × 1 （P19388） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （P61218） DNA-directed RNA polymerase II subunit RPB7 × 1 （P62487） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （P52434） DNA-directed RNA polymerase II subunit RPB9 × 1 （P36954） DNA-directed RNA polymerases I, II, and III subunit RPABC5 × 1 （P62875） DNA-directed RNA polymerase II subunit RPB11-a × 1 （P52435） Non-template DNA, nucleic acid scaffold × 1 RNA, nucleic acid scaffold × 1 Template DNA, nucleic acid scaffold × 1 ATP-dependent DNA helicase Q5 × 1 （O94762） ZINC ION × 8 MAGNESIUM ION × 1 ADENOSINE-5'-DIPHOSPHATE × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPAB4_HUMAN
Isoform	—
PDB entities	12
Chains and sequence ranges	Author chain L； PDBConstruct 1–58； UniProt 1–58

ATP-dependent DNA helicase Q5

Homo sapiens

UniProt O94762

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 13 DNA 2 RNA 1	DNA-directed RNA polymerase II subunit RPB1 × 1 （P24928） DNA-directed RNA polymerase II subunit RPB2 × 1 （P30876） DNA-directed RNA polymerase II subunit RPB3 × 1 （P19387） DNA-directed RNA polymerase II subunit RPB4 × 1 （O15514） DNA-directed RNA polymerases I, II, and III subunit RPABC1 × 1 （P19388） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （P61218） DNA-directed RNA polymerase II subunit RPB7 × 1 （P62487） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （P52434） DNA-directed RNA polymerase II subunit RPB9 × 1 （P36954） DNA-directed RNA polymerases I, II, and III subunit RPABC5 × 1 （P62875） DNA-directed RNA polymerase II subunit RPB11-a × 1 （P52435） DNA-directed RNA polymerases I, II, and III subunit RPABC4 × 1 （P53803） Non-template DNA, nucleic acid scaffold × 1 RNA, nucleic acid scaffold × 1 Template DNA, nucleic acid scaffold × 1 ZINC ION × 8 MAGNESIUM ION × 1 ADENOSINE-5'-DIPHOSPHATE × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RECQ5_HUMAN
Isoform	—
PDB entities	16
Chains and sequence ranges	Author chain U； PDBConstruct 1–991； UniProt 1–991

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	9ei4
Deposition date deposition_date	2024-11-25
Structure title title	Cryo-EM structure of Human RNA polymerase II Elongation Complex bound to the RECQL5 helicase in the presence of ADP
Keywords keywords	translocation, Human RNA polymerase II, RECQL5 helicase, TRANSCRIPTION, TRANSFERASE-DNA-RNA complex; TRANSCRIPTION, TRANSFERASE/DNA/RNA
Experimental Method method	ELECTRON MICROSCOPY

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

9ei4__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

9ei4__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

9ei4__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	53.53 Å
Rg (electron density)	53.55 Å
Total Rg	53.63 Å
Atom count	35848
Residues	4419
Excluded volume	635980 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`9ei4__assembly_1__model_1`	hexadecameric （16）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (19)

Entity 1 Entity 1 polymer

Description description	DNA-directed RNA polymerase II subunit RPB1
Molecular weight formula_weight	217420.0 kDa
EC 编号 ec_number	2.7.7.6
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A
Atom count atom_count	35854

MHGGGPPSGDSACPLRTIKRVQFGVLSPDELKRMSVTEGGIKYPETTEGGRPKLGGLMDPRQGVIERTGRCQTCAGNMTECPGHFGHIELAKPVFHVGFLVKTMKVLRCVCFFCSKLLVDSNNPKIKDILAKSKGQPKKRLTHVYDLCKGKNICEGGEEMDNKFGVEQPEGDEDLTKEKGHGGCGRYQPRIRRSGLELYAEWKHVNEDSQEKKILLSPERVHEIFKRISDEECFVLGMEPRYARPEWMIVTVLPVPPLSVRPAVVMQGSARNQDDLTHKLADIVKINNQLRRNEQNGAAAHVIAEDVKLLQFHVATMVDNELPGLPRAMQKSGRPLKSLKQRLKGKEGRVRGNLMGKRVDFSARTVITPDPNLSIDQVGVPRSIAANMTFAEIVTPFNIDRLQELVRRGNSQYPGAKYIIRDNGDRIDLRFHPKPSDLHLQTGYKVERHMCDGDIVIFNRQPTLHKMSMMGHRVRILPWSTFRLNLSVTTPYNADFDGDEMNLHLPQSLETRAEIQELAMVPRMIVTPQSNRPVMGIVQDTLTAVRKFTKRDVFLERGEVMNLLMFLSTWDGKVPQPAILKPRPLWTGKQIFSLIIPGHINCIRTHSTHPDDEDSGPYKHISPGDTKVVVENGELIMGILCKKSLGTSAGSLVHISYLEMGHDITRLFYSNIQTVINNWLLIEGHTIGIGDSIADSKTYQDIQNTIKKAKQDVIEVIEKAHNNELEPTPGNTLRQTFENQVNRILNDARDKTGSSAQKSLSEYNNFKSMVVSGAKGSKINISQVIAVVGQQNVEGKRIPFGFKHRTLPHFIKDDYGPESRGFVENSYLAGLTPTEFFFHAMGGREGLIDTAVKTAETGYIQRRLIKSMESVMVKYDATVRNSINQVVQLRYGEDGLAGESVEFQNLATLKPSNKAFEKKFRFDYTNERALRRTLQEDLVKDVLSNAHIQNELEREFERMREDREVLRVIFPTGDSKVVLPCNLLRMIWNAQKIFHINPRLPSDLHPIKVVEGVKELSKKLVIVNGDDPLSRQAQENATLLFNIHLRSTLCSRRMAEEFRLSGEAFDWLLGEIESKFNQAIAHPGEMVGALAAQSLGEPATQMTLNTFHYAGVSAKNVTLGVPRLKELINISKKPKTPSLTVFLLGQSARDAERAKDILCRLEHTTLRKVTANTAIYYDPNPQSTVVAEDQEWVNVYYEMPDFDVARISPWLLRVELDRKHMTDRKLTMEQIAEKINAGFGDDLNCIFNDDNAEKLVLRIRIMNSDENKMQEEEEVVDKMDDDVFLRCIESNMLTDMTLQGIEQISKVYMHLPQTDNKKKIIITEDGEFKALQEWILETDGVSLMRVLSEKDVDPVRTTSNDIVEIFTVLGIEAVRKALERELYHVISFDGSYVNYRHLALLCDTMTCRGHLMAITRHGVNRQDTGPLMKCSFEETVDVLMEAAAHGESDPMKGVSENIMLGQLAPAGTGCFDLLLDAEKCKYGMEIPTNIPGLGAAGPTGMFFGSAPSPMGGISPAMTPWNQGATPAYGAWSPSVGSGMTPGAAGFSPSAASDASGFSPGYSPAWSPTPGSPGSPGPSSPYIPSPGGAMSPSYSPTSPAYEPRSPGGYTPQSPSYSPTSPSYSPTSPSYSPTSPNYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPNYSPTSPNYTPTSPSYSPTSPSYSPTSPNYTPTSPNYSPTSPSYSPTSPSYSPTSPSYSPSSPRYTPQSPTYTPSSPSYSPSSPSYSPASPKYTPTSPSYSPSSPEYTPTSPKYSPTSPKYSPTSPKYSPTSPTYSPTTPKYSPTSPTYSPTSPVYTPTSPKYSPTSPTYSPTSPKYSPTSPTYSPTSPKGSTYSPTSPGYSPTSPTYSLTSPAISPDDSDEEN

Entity 2 Entity 2 polymer

Description description	DNA-directed RNA polymerase II subunit RPB2
Molecular weight formula_weight	134071.5 kDa
EC 编号 ec_number	2.7.7.6
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	B

MYDADEDMQYDEDDDEITPDLWQEACWIVISSYFDEKGLVRQQLDSFDEFIQMSVQRIVEDAPPIDLQAEAQHASGEVEEPPRYLLKFEQIYLSKPTHWERDGAPSPMMPNEARLRNLTYSAPLYVDITKTVIKEGEEQLQTQHQKTFIGKIPIMLRSTYCLLNGLTDRDLCELNECPLDPGGYFIINGSEKVLIAQEKMATNTVYVFAKKDSKYAYTGECRSCLENSSRPTSTIWVSMLARGGQGAKKSAIGQRIVATLPYIKQEVPIIIVFRALGFVSDRDILEHIIYDFEDPEMMEMVKPSLDEAFVIQEQNVALNFIGSRGAKPGVTKEKRIKYAKEVLQKEMLPHVGVSDFCETKKAYFLGYMVHRLLLAALGRRELDDRDHYGNKRLDLAGPLLAFLFRGMFKNLLKEVRIYAQKFIDRGKDFNLELAIKTRIISDGLKYSLATGNWGDQKKAHQARAGVSQVLNRLTFASTLSHLRRLNSPIGRDGKLAKPRQLHNTLWGMVCPAETPEGHAVGLVKNLALMAYISVGSQPSPILEFLEEWSMENLEEISPAAIADATKIFVNGCWVGIHKDPEQLMNTLRKLRRQMDIIVSEVSMIRDIREREIRIYTDAGRICRPLLIVEKQKLLLKKRHIDQLKEREYNNYSWQDLVASGVVEYIDTLEEETVMLAMTPDDLQEKEVAYCSTYTHCEIHPSMILGVCASIIPFPDHNQSPRNTYQSAMGKQAMGVYITNFHVRMDTLAHVLYYPQKPLVTTRSMEYLRFRELPAGINSIVAIASYTGYNQEDSVIMNRSAVDRGFFRSVFYRSYKEQESKKGFDQEEVFEKPTRETCQGMRHAIYDKLDDDGLIAPGVRVSGDDVIIGKTVTLPENEDELESTNRRYTKRDCSTFLRTSETGIVDQVMVTLNQEGYKFCKIRVRSVRIPQIGDKFASRHGQKGTCGIQYRQEDMPFTCEGITPDIIINPHAIPSRMTIGHLIECLQGKVSANKGEIGDATPFNDAVNVQKISNLLSDYGYHLRGNEVLYNGFTGRKITSQIFIGPTYYQRLKHMVDDKIHSRARGPIQILNRQPMEGRSRDGGLRFGEMERDCQIAHGAAQFLRERLFEASDPYQVHVCNLCGIMAIANTRTHTYECRGCRNKTQISLVRMPYACKLLFQELMSMSIAPRMMSV

Entity 3 Entity 3 polymer

Description description	DNA-directed RNA polymerase II subunit RPB3
Molecular weight formula_weight	31478.1 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	C

MPYANQPTVRITELTDENVKFIIENTDLAVANSIRRVFIAEVPIIAIDWVQIDANSSVLHDEFIAHRLGLIPLISDDIVDKLQYSRDCTCEEFCPECSVEFTLDVRCNEDQTRHVTSRDLISNSPRVIPVTSRNRDNDPNDYVEQDDILIVKLRKGQELRLRAYAKKGFGKEHAKWNPTAGVAFEYDPDNALRHTVYPKPEEWPKSEYSELDEDESQAPYDPNGKPERFYYNVESCGSLRPETIVLSALSGLKKKLSDLQTQLSHEIQSDVLTIN

Entity 4 Entity 4 polymer

Description description	DNA-directed RNA polymerase II subunit RPB4
Molecular weight formula_weight	16331.3 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	D

MAAGGSDPRAGDVEEDASQLIFPKEFETAETLLNSEVHMLLEHRKQQNESAEDEQELSEVFMKTLNYTARFSRFKNRETIASVRSLLLQKKLHKFELACLANLCPETAEESKALIPSLEGRFEDEELQQILDDIQTKRSFQY

Entity 5 Entity 5 polymer

Description description	DNA-directed RNA polymerases I, II, and III subunit RPABC1
Molecular weight formula_weight	24644.3 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	E

MDDEEETYRLWKIRKTIMQLCHDRGYLVTQDELDQTLEEFKAQFGDKPSEGRPRRTDLTVLVAHNDDPTDQMFVFFPEEPKVGIKTIKVYCQRMQEENITRALIVVQQGMTPSAKQSLVDMAPKYILEQFLQQELLINITEHELVPEHVVMTKEEVTELLARYKLRENQLPRIQAGDPVARYFGIKRGQVVKIIRPSETAGRYITYRLVQ

Entity 6 Entity 6 polymer

Description description	DNA-directed RNA polymerases I, II, and III subunit RPABC2
Molecular weight formula_weight	14491.0 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	F

MSDNEDNFDGDDFDDVEEDEGLDDLENAEEEGQENVEILPSGERPQANQKRITTPYMTKYERARVLGTRALQIAMCAPVMVELEGETDPLLIAMKELKARKIPIIIRRYLPDGSYEDWGVDELIITD

Entity 7 Entity 7 polymer

Description description	DNA-directed RNA polymerase II subunit RPB7
Molecular weight formula_weight	19314.3 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	G

MFYHISLEHEILLHPRYFGPNLLNTVKQKLFTEVEGTCTGKYGFVIAVTTIDNIGAGVIQPGRGFVLYPVKYKAIVFRPFKGEVVDAVVTQVNKVGLFTEIGPMSCFISRHSIPSEMEFDPNSNPPCYKTMDEDIVIQQDDEIRLKIVGTRVDKNDIFAIGSLMDDYLGLVS

Entity 8 Entity 8 polymer

Description description	DNA-directed RNA polymerases I, II, and III subunit RPABC3
Molecular weight formula_weight	17162.3 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	H

MAGILFEDIFDVKDIDPEGKKFDRVSRLHCESESFKMDLILDVNIQIYPVDLGDKFRLVIASTLYEDGTLDDGEYNPTDDRPSRADQFEYVMYGKVYRIEGDETSTEAATRLSAYVSYGGLLMRLQGDANNLHGFEVDSRVYLLMKKLAF

Entity 9 Entity 9 polymer

Description description	DNA-directed RNA polymerase II subunit RPB9
Molecular weight formula_weight	14541.2 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	I

MEPDGTYEPGFVGIRFCQECNNMLYPKEDKENRILLYACRNCDYQQEADNSCIYVNKITHEVDELTQIIADVSQDPTLPRTEDHPCQKCGHKEAVFFQSHSARAEDAMRLYYVCTAPHCGHRWTE

Entity 10 Entity 10 polymer

Description description	DNA-directed RNA polymerases I, II, and III subunit RPABC5
Molecular weight formula_weight	7655.1 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	J

MIIPVRCFTCGKIVGNKWEAYLGLLQAEYTEGDALDALGLKRYCCRRMLLAHVDLIEKLLNYAPLEK

Entity 11 Entity 11 polymer

Description description	DNA-directed RNA polymerase II subunit RPB11-a
Molecular weight formula_weight	13310.3 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	K

MNAPPAFESFLLFEGEKKITINKDTKVPNACLFTINKEDHTLGNIIKSQLLKDPQVLFAGYKVPHPLEHKIIIRVQTTPDYSPQEAFTNAITDLISELSLLEERFRVAIKDKQEGIE

Entity 12 Entity 12 polymer

Description description	DNA-directed RNA polymerases I, II, and III subunit RPABC4
Molecular weight formula_weight	7018.2 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	L

MDTQKDVQPPKQQPMIYICGECHTENEIKSRDPIRCRECGYRIMYKKRTKRLVVFDAR

Entity 13 Entity 13 polymer

Description description	Non-template DNA, nucleic acid scaffold
Molecular weight formula_weight	13305.6 kDa
Source method src_method	syn
Copy count entity_count	1

Polymer Polymer

Polymer type type	polydeoxyribonucleotide
Chain IDs strand_id	N

TAGTAAACTAGTATTGAAAGTACTTGAGCTTAGACAGCATGTC

Entity 14 Entity 14 polymer

Description description	RNA, nucleic acid scaffold
Molecular weight formula_weight	6414.9 kDa
Source method src_method	syn
Copy count entity_count	1

Polymer Polymer

Polymer type type	polyribonucleotide
Chain IDs strand_id	P

UAUAUGCAUAAAGACCAGGC

Entity 15 Entity 15 polymer

Description description	Template DNA, nucleic acid scaffold
Molecular weight formula_weight	8515.5 kDa
Source method src_method	syn
Copy count entity_count	1

Polymer Polymer

Polymer type type	polydeoxyribonucleotide
Chain IDs strand_id	T

CTCAAGTACTTACGCCTGGTCATTACTA

Entity 16 Entity 16 polymer

Description description	ATP-dependent DNA helicase Q5
Molecular weight formula_weight	109024.9 kDa
EC 编号 ec_number	5.6.2.4
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	U

MSSHHTTFPFDPERRVRSTLKKVFGFDSFKTPLQESATMAVVKGNKDVFVCMPTGAGKSLCYQLPALLAKGITIVVSPLIALIQDQVDHLLTLKVRVSSLNSKLSAQERKELLADLEREKPQTKILYITPEMAASSSFQPTLNSLVSRHLLSYLVVDEAHCVSQWGHDFRPDYLRLGALRSRLGHAPCVALTATATPQVQEDVFAALHLKKPVAIFKTPCFRANLFYDVQFKELISDPYGNLKDFCLKALGQEADKGLSGCGIVYCRTREACEQLAIELSCRGVNAKAYHAGLKASERTLVQNDWMEEKVPVIVATISFGMGVDKANVRFVAHWNIAKSMAGYYQESGRAGRDGKPSWCRLYYSRNDRDQVSFLIRKEVAKLQEKRGNKASDKATIMAFDALVTFCEELGCRHAAIAKYFGDALPACAKGCDHCQNPTAVRRRLEALERSSSWSKTCIGPSQGNGFDPELYEGGRKGYGDFSRYDEGSGGSGDEGRDEAHKREWNLFYQKQMQLRKGKDPKIEEFVPPDENCPLKEASSRRIPRLTVKAREHCLRLLEEALSSNRQSTRTADEADLRAKAVELEHETFRNAKVANLYKASVLKKVADIHRASKDGQPYDMGGSAKSCSAQAEPPEPNEYDIPPASHVYSLKPKRVGAGFPKGSCPFQTATELMETTRIREQAPQPERGGEHEPPSRPCGLLDEDGSEPLPGPRGEVPGGSAHYGGPSPEKKAKSSSGGSSLAKGRASKKQQLLATAAHKDSQSIARFFCRRVESPALLASAPEAEGACPSCEGVQGPPMAPEKYTGEEDGAGGHSPAPPQTEECLRERPSTCPPRDQGTPEVQPTPAKDTWKGKRPRSQQENPESQPQKRPRPSAKPSVVAEVKGSVSASEQGTLNPTAQDPFQLSAPGVSLKEAANVVVKCLTPFYKEGKFASKELFKGFARHLSHLLTQKTSPGRSVKEEAQNLIRHFFHGRARCESEADWHGLCGPQR

Source Source

Source organism organism	Homo sapiens
Common name common_name	human
NCBI taxonomy ID ncbi_taxonomy_id	9606
Host organism host_organism	Escherichia coli BL21(DE3)

Entity 17 Entity 17 non-polymer

Description description	ZINC ION
Molecular weight formula_weight	65.4 kDa
Source method src_method	syn
Copy count entity_count	8

Entity 18 Entity 18 non-polymer

Description description	MAGNESIUM ION
Molecular weight formula_weight	24.3 kDa
Source method src_method	syn
Copy count entity_count	1

Entity 19 Entity 19 non-polymer

Description description	ADENOSINE-5'-DIPHOSPHATE
Molecular weight formula_weight	427.2 kDa
Source method src_method	syn
Copy count entity_count	1

▶

Unit-cell length a length_a	1.000 Å
Unit-cell length b length_b	1.000 Å
Unit-cell length c length_c	1.000 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	—

Space group space_group	P 1
Space-group number int_tables_number	1
Crystal system crystal_system	triclinic

Resolution resolution	3.70 Å
Resolution测定Method resolution_method	—
颗粒数 num_particles	17442

Cryo-EM structure of Human RNA polymerase II Elongation Complex bound to the RECQL5 helicase in the presence of ADP

1. Protein Identity and Related Structures Protein Identity & Related Structures

DNA-directed RNA polymerase II subunit RPB1

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

DNA-directed RNA polymerase II subunit RPB2

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

DNA-directed RNA polymerase II subunit RPB3

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

DNA-directed RNA polymerase II subunit RPB4

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

DNA-directed RNA polymerases I, II, and III subunit RPABC1

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

DNA-directed RNA polymerases I, II, and III subunit RPABC2

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

DNA-directed RNA polymerase II subunit RPB7

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

DNA-directed RNA polymerases I, II, and III subunit RPABC3

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

DNA-directed RNA polymerase II subunit RPB9

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

DNA-directed RNA polymerases I, II, and III subunit RPABC5

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer