9fda

Structure of E. coli 30S-IF1-IF3-mRNA-Edeine complex

Method: ELECTRON MICROSCOPY

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1. Protein Identity and Related Structures Protein Identity & Related Structures

Small ribosomal subunit protein uS4

Escherichia coli

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 14 RNA 1	Small ribosomal subunit protein uS5 × 1 （P0A7W1） Small ribosomal subunit protein bS6, fully modified isoform × 1 （P02358） Small ribosomal subunit protein uS8 × 1 （P0A7W7） Translation initiation factor IF-1 × 1 （P69222） Translation initiation factor IF-3 × 1 （P0A707） Small ribosomal subunit protein uS11 × 1 （P0A7R9） Small ribosomal subunit protein uS12 × 1 （P0A7S3） Small ribosomal subunit protein uS15 × 1 （P0ADZ4） Small ribosomal subunit protein bS16 × 1 （P0A7T3） Small ribosomal subunit protein uS17 × 1 （P0AG63） Small ribosomal subunit protein bS18 × 1 （P0A7T7） Small ribosomal subunit protein bS20 × 1 （P0A7U7） Small ribosomal subunit protein bS21 × 1 （P68679） 16S rRNA × 1 POTASSIUM ION × 25 MAGNESIUM ION × 53 EDEINE B × 1 water × 15	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RS4_ECOLI
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain D； PDBConstruct 1–206； UniProt 1–206

Small ribosomal subunit protein uS5

Escherichia coli

UniProt P0A7W1

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 14 RNA 1	Small ribosomal subunit protein uS4 × 1 （P0A7V8） Small ribosomal subunit protein bS6, fully modified isoform × 1 （P02358） Small ribosomal subunit protein uS8 × 1 （P0A7W7） Translation initiation factor IF-1 × 1 （P69222） Translation initiation factor IF-3 × 1 （P0A707） Small ribosomal subunit protein uS11 × 1 （P0A7R9） Small ribosomal subunit protein uS12 × 1 （P0A7S3） Small ribosomal subunit protein uS15 × 1 （P0ADZ4） Small ribosomal subunit protein bS16 × 1 （P0A7T3） Small ribosomal subunit protein uS17 × 1 （P0AG63） Small ribosomal subunit protein bS18 × 1 （P0A7T7） Small ribosomal subunit protein bS20 × 1 （P0A7U7） Small ribosomal subunit protein bS21 × 1 （P68679） 16S rRNA × 1 POTASSIUM ION × 25 MAGNESIUM ION × 53 EDEINE B × 1 water × 15	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RS5_ECOLI
Isoform	—
PDB entities	2
Chains and sequence ranges	Author chain E； PDBConstruct 1–167； UniProt 1–167

Small ribosomal subunit protein bS6, fully modified isoform

Escherichia coli

UniProt P02358

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 14 RNA 1	Small ribosomal subunit protein uS4 × 1 （P0A7V8） Small ribosomal subunit protein uS5 × 1 （P0A7W1） Small ribosomal subunit protein uS8 × 1 （P0A7W7） Translation initiation factor IF-1 × 1 （P69222） Translation initiation factor IF-3 × 1 （P0A707） Small ribosomal subunit protein uS11 × 1 （P0A7R9） Small ribosomal subunit protein uS12 × 1 （P0A7S3） Small ribosomal subunit protein uS15 × 1 （P0ADZ4） Small ribosomal subunit protein bS16 × 1 （P0A7T3） Small ribosomal subunit protein uS17 × 1 （P0AG63） Small ribosomal subunit protein bS18 × 1 （P0A7T7） Small ribosomal subunit protein bS20 × 1 （P0A7U7） Small ribosomal subunit protein bS21 × 1 （P68679） 16S rRNA × 1 POTASSIUM ION × 25 MAGNESIUM ION × 53 EDEINE B × 1 water × 15	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RS6_ECOLI
Isoform	—
PDB entities	3
Chains and sequence ranges	Author chain F； PDBConstruct 1–135； UniProt 1–135

Small ribosomal subunit protein uS8

Escherichia coli

UniProt P0A7W7

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 14 RNA 1	Small ribosomal subunit protein uS4 × 1 （P0A7V8） Small ribosomal subunit protein uS5 × 1 （P0A7W1） Small ribosomal subunit protein bS6, fully modified isoform × 1 （P02358） Translation initiation factor IF-1 × 1 （P69222） Translation initiation factor IF-3 × 1 （P0A707） Small ribosomal subunit protein uS11 × 1 （P0A7R9） Small ribosomal subunit protein uS12 × 1 （P0A7S3） Small ribosomal subunit protein uS15 × 1 （P0ADZ4） Small ribosomal subunit protein bS16 × 1 （P0A7T3） Small ribosomal subunit protein uS17 × 1 （P0AG63） Small ribosomal subunit protein bS18 × 1 （P0A7T7） Small ribosomal subunit protein bS20 × 1 （P0A7U7） Small ribosomal subunit protein bS21 × 1 （P68679） 16S rRNA × 1 POTASSIUM ION × 25 MAGNESIUM ION × 53 EDEINE B × 1 water × 15	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RS8_ECOLI
Isoform	—
PDB entities	4
Chains and sequence ranges	Author chain H； PDBConstruct 1–130； UniProt 1–130

Translation initiation factor IF-1

Escherichia coli

UniProt P69222

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 14 RNA 1	Small ribosomal subunit protein uS4 × 1 （P0A7V8） Small ribosomal subunit protein uS5 × 1 （P0A7W1） Small ribosomal subunit protein bS6, fully modified isoform × 1 （P02358） Small ribosomal subunit protein uS8 × 1 （P0A7W7） Translation initiation factor IF-3 × 1 （P0A707） Small ribosomal subunit protein uS11 × 1 （P0A7R9） Small ribosomal subunit protein uS12 × 1 （P0A7S3） Small ribosomal subunit protein uS15 × 1 （P0ADZ4） Small ribosomal subunit protein bS16 × 1 （P0A7T3） Small ribosomal subunit protein uS17 × 1 （P0AG63） Small ribosomal subunit protein bS18 × 1 （P0A7T7） Small ribosomal subunit protein bS20 × 1 （P0A7U7） Small ribosomal subunit protein bS21 × 1 （P68679） 16S rRNA × 1 POTASSIUM ION × 25 MAGNESIUM ION × 53 EDEINE B × 1 water × 15	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	IF1_ECOLI
Isoform	—
PDB entities	5
Chains and sequence ranges	Author chain I； PDBConstruct 1–72； UniProt 1–72

Translation initiation factor IF-3

Escherichia coli

UniProt P0A707

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 14 RNA 1	Small ribosomal subunit protein uS4 × 1 （P0A7V8） Small ribosomal subunit protein uS5 × 1 （P0A7W1） Small ribosomal subunit protein bS6, fully modified isoform × 1 （P02358） Small ribosomal subunit protein uS8 × 1 （P0A7W7） Translation initiation factor IF-1 × 1 （P69222） Small ribosomal subunit protein uS11 × 1 （P0A7R9） Small ribosomal subunit protein uS12 × 1 （P0A7S3） Small ribosomal subunit protein uS15 × 1 （P0ADZ4） Small ribosomal subunit protein bS16 × 1 （P0A7T3） Small ribosomal subunit protein uS17 × 1 （P0AG63） Small ribosomal subunit protein bS18 × 1 （P0A7T7） Small ribosomal subunit protein bS20 × 1 （P0A7U7） Small ribosomal subunit protein bS21 × 1 （P68679） 16S rRNA × 1 POTASSIUM ION × 25 MAGNESIUM ION × 53 EDEINE B × 1 water × 15	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	IF3_ECOLI
Isoform	—
PDB entities	6
Chains and sequence ranges	Author chain J； PDBConstruct 1–180； UniProt 1–180

Small ribosomal subunit protein uS11

Escherichia coli

UniProt P0A7R9

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 14 RNA 1	Small ribosomal subunit protein uS4 × 1 （P0A7V8） Small ribosomal subunit protein uS5 × 1 （P0A7W1） Small ribosomal subunit protein bS6, fully modified isoform × 1 （P02358） Small ribosomal subunit protein uS8 × 1 （P0A7W7） Translation initiation factor IF-1 × 1 （P69222） Translation initiation factor IF-3 × 1 （P0A707） Small ribosomal subunit protein uS12 × 1 （P0A7S3） Small ribosomal subunit protein uS15 × 1 （P0ADZ4） Small ribosomal subunit protein bS16 × 1 （P0A7T3） Small ribosomal subunit protein uS17 × 1 （P0AG63） Small ribosomal subunit protein bS18 × 1 （P0A7T7） Small ribosomal subunit protein bS20 × 1 （P0A7U7） Small ribosomal subunit protein bS21 × 1 （P68679） 16S rRNA × 1 POTASSIUM ION × 25 MAGNESIUM ION × 53 EDEINE B × 1 water × 15	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RS11_ECOLI
Isoform	—
PDB entities	7
Chains and sequence ranges	Author chain K； PDBConstruct 1–129； UniProt 1–129

Small ribosomal subunit protein uS12

Escherichia coli

UniProt P0A7S3

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 14 RNA 1	Small ribosomal subunit protein uS4 × 1 （P0A7V8） Small ribosomal subunit protein uS5 × 1 （P0A7W1） Small ribosomal subunit protein bS6, fully modified isoform × 1 （P02358） Small ribosomal subunit protein uS8 × 1 （P0A7W7） Translation initiation factor IF-1 × 1 （P69222） Translation initiation factor IF-3 × 1 （P0A707） Small ribosomal subunit protein uS11 × 1 （P0A7R9） Small ribosomal subunit protein uS15 × 1 （P0ADZ4） Small ribosomal subunit protein bS16 × 1 （P0A7T3） Small ribosomal subunit protein uS17 × 1 （P0AG63） Small ribosomal subunit protein bS18 × 1 （P0A7T7） Small ribosomal subunit protein bS20 × 1 （P0A7U7） Small ribosomal subunit protein bS21 × 1 （P68679） 16S rRNA × 1 POTASSIUM ION × 25 MAGNESIUM ION × 53 EDEINE B × 1 water × 15	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RS12_ECOLI
Isoform	—
PDB entities	8
Chains and sequence ranges	Author chain L； PDBConstruct 1–124； UniProt 1–124

Small ribosomal subunit protein uS15

Escherichia coli

UniProt P0ADZ4

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 14 RNA 1	Small ribosomal subunit protein uS4 × 1 （P0A7V8） Small ribosomal subunit protein uS5 × 1 （P0A7W1） Small ribosomal subunit protein bS6, fully modified isoform × 1 （P02358） Small ribosomal subunit protein uS8 × 1 （P0A7W7） Translation initiation factor IF-1 × 1 （P69222） Translation initiation factor IF-3 × 1 （P0A707） Small ribosomal subunit protein uS11 × 1 （P0A7R9） Small ribosomal subunit protein uS12 × 1 （P0A7S3） Small ribosomal subunit protein bS16 × 1 （P0A7T3） Small ribosomal subunit protein uS17 × 1 （P0AG63） Small ribosomal subunit protein bS18 × 1 （P0A7T7） Small ribosomal subunit protein bS20 × 1 （P0A7U7） Small ribosomal subunit protein bS21 × 1 （P68679） 16S rRNA × 1 POTASSIUM ION × 25 MAGNESIUM ION × 53 EDEINE B × 1 water × 15	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RS15_ECOLI
Isoform	—
PDB entities	9
Chains and sequence ranges	Author chain O； PDBConstruct 1–89； UniProt 1–89

Small ribosomal subunit protein bS16

Escherichia coli

UniProt P0A7T3

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 14 RNA 1	Small ribosomal subunit protein uS4 × 1 （P0A7V8） Small ribosomal subunit protein uS5 × 1 （P0A7W1） Small ribosomal subunit protein bS6, fully modified isoform × 1 （P02358） Small ribosomal subunit protein uS8 × 1 （P0A7W7） Translation initiation factor IF-1 × 1 （P69222） Translation initiation factor IF-3 × 1 （P0A707） Small ribosomal subunit protein uS11 × 1 （P0A7R9） Small ribosomal subunit protein uS12 × 1 （P0A7S3） Small ribosomal subunit protein uS15 × 1 （P0ADZ4） Small ribosomal subunit protein uS17 × 1 （P0AG63） Small ribosomal subunit protein bS18 × 1 （P0A7T7） Small ribosomal subunit protein bS20 × 1 （P0A7U7） Small ribosomal subunit protein bS21 × 1 （P68679） 16S rRNA × 1 POTASSIUM ION × 25 MAGNESIUM ION × 53 EDEINE B × 1 water × 15	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RS16_ECOLI
Isoform	—
PDB entities	10
Chains and sequence ranges	Author chain P； PDBConstruct 1–82； UniProt 1–82

Small ribosomal subunit protein uS17

Escherichia coli

UniProt P0AG63

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 14 RNA 1	Small ribosomal subunit protein uS4 × 1 （P0A7V8） Small ribosomal subunit protein uS5 × 1 （P0A7W1） Small ribosomal subunit protein bS6, fully modified isoform × 1 （P02358） Small ribosomal subunit protein uS8 × 1 （P0A7W7） Translation initiation factor IF-1 × 1 （P69222） Translation initiation factor IF-3 × 1 （P0A707） Small ribosomal subunit protein uS11 × 1 （P0A7R9） Small ribosomal subunit protein uS12 × 1 （P0A7S3） Small ribosomal subunit protein uS15 × 1 （P0ADZ4） Small ribosomal subunit protein bS16 × 1 （P0A7T3） Small ribosomal subunit protein bS18 × 1 （P0A7T7） Small ribosomal subunit protein bS20 × 1 （P0A7U7） Small ribosomal subunit protein bS21 × 1 （P68679） 16S rRNA × 1 POTASSIUM ION × 25 MAGNESIUM ION × 53 EDEINE B × 1 water × 15	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RS17_ECOLI
Isoform	—
PDB entities	11
Chains and sequence ranges	Author chain Q； PDBConstruct 1–84； UniProt 1–84

Small ribosomal subunit protein bS18

Escherichia coli

UniProt P0A7T7

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 14 RNA 1	Small ribosomal subunit protein uS4 × 1 （P0A7V8） Small ribosomal subunit protein uS5 × 1 （P0A7W1） Small ribosomal subunit protein bS6, fully modified isoform × 1 （P02358） Small ribosomal subunit protein uS8 × 1 （P0A7W7） Translation initiation factor IF-1 × 1 （P69222） Translation initiation factor IF-3 × 1 （P0A707） Small ribosomal subunit protein uS11 × 1 （P0A7R9） Small ribosomal subunit protein uS12 × 1 （P0A7S3） Small ribosomal subunit protein uS15 × 1 （P0ADZ4） Small ribosomal subunit protein bS16 × 1 （P0A7T3） Small ribosomal subunit protein uS17 × 1 （P0AG63） Small ribosomal subunit protein bS20 × 1 （P0A7U7） Small ribosomal subunit protein bS21 × 1 （P68679） 16S rRNA × 1 POTASSIUM ION × 25 MAGNESIUM ION × 53 EDEINE B × 1 water × 15	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RS18_ECOLI
Isoform	—
PDB entities	12
Chains and sequence ranges	Author chain R； PDBConstruct 1–75； UniProt 1–75

Small ribosomal subunit protein bS20

Escherichia coli

UniProt P0A7U7

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 14 RNA 1	Small ribosomal subunit protein uS4 × 1 （P0A7V8） Small ribosomal subunit protein uS5 × 1 （P0A7W1） Small ribosomal subunit protein bS6, fully modified isoform × 1 （P02358） Small ribosomal subunit protein uS8 × 1 （P0A7W7） Translation initiation factor IF-1 × 1 （P69222） Translation initiation factor IF-3 × 1 （P0A707） Small ribosomal subunit protein uS11 × 1 （P0A7R9） Small ribosomal subunit protein uS12 × 1 （P0A7S3） Small ribosomal subunit protein uS15 × 1 （P0ADZ4） Small ribosomal subunit protein bS16 × 1 （P0A7T3） Small ribosomal subunit protein uS17 × 1 （P0AG63） Small ribosomal subunit protein bS18 × 1 （P0A7T7） Small ribosomal subunit protein bS21 × 1 （P68679） 16S rRNA × 1 POTASSIUM ION × 25 MAGNESIUM ION × 53 EDEINE B × 1 water × 15	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RS20_ECOLI
Isoform	—
PDB entities	13
Chains and sequence ranges	Author chain T； PDBConstruct 1–87； UniProt 1–87

Small ribosomal subunit protein bS21

Escherichia coli

UniProt P68679

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 14 RNA 1	Small ribosomal subunit protein uS4 × 1 （P0A7V8） Small ribosomal subunit protein uS5 × 1 （P0A7W1） Small ribosomal subunit protein bS6, fully modified isoform × 1 （P02358） Small ribosomal subunit protein uS8 × 1 （P0A7W7） Translation initiation factor IF-1 × 1 （P69222） Translation initiation factor IF-3 × 1 （P0A707） Small ribosomal subunit protein uS11 × 1 （P0A7R9） Small ribosomal subunit protein uS12 × 1 （P0A7S3） Small ribosomal subunit protein uS15 × 1 （P0ADZ4） Small ribosomal subunit protein bS16 × 1 （P0A7T3） Small ribosomal subunit protein uS17 × 1 （P0AG63） Small ribosomal subunit protein bS18 × 1 （P0A7T7） Small ribosomal subunit protein bS20 × 1 （P0A7U7） 16S rRNA × 1 POTASSIUM ION × 25 MAGNESIUM ION × 53 EDEINE B × 1 water × 15	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RS21_ECOLI
Isoform	—
PDB entities	14
Chains and sequence ranges	Author chain U； PDBConstruct 1–71； UniProt 1–71

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	9fda
Deposition date deposition_date	2024-05-16
Last revision last_revision	2025-03-19
Structure title title	Structure of E. coli 30S-IF1-IF3-mRNA-Edeine complex
Keywords keywords	Ribosomes, 30S subunit, Antibiotic, 30S inhibitors, TRANSLATION; TRANSLATION
Experimental Method method	ELECTRON MICROSCOPY

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

9fda__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

9fda__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

9fda__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	55.52 Å
Rg (electron density)	56.19 Å
Total Rg	56.19 Å
Atom count	34224
Residues	2499
Excluded volume	527870 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`9fda__assembly_1__model_1`	15-meric （15）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (19)

Entity 1 Entity 1 polymer

Description description	Small ribosomal subunit protein uS4
Molecular weight formula_weight	23514.2 kDa
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	D
Atom count atom_count	36060

MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGARKPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVVYRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALELAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK

Source Source

Source organism organism	Escherichia coli
NCBI taxonomy ID ncbi_taxonomy_id	562
Host organism host_organism	Escherichia coli

Entity 2 Entity 2 polymer

Description description	Small ribosomal subunit protein uS5
Molecular weight formula_weight	17629.4 kDa
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	E

MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNGRVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEGTGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKSVEEILGK

Source Source

Source organism organism	Escherichia coli
NCBI taxonomy ID ncbi_taxonomy_id	562
Host organism host_organism	Escherichia coli

Entity 3 Entity 3 polymer

Description description	Small ribosomal subunit protein bS6, fully modified isoform
Molecular weight formula_weight	15727.5 kDa
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	F

MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGRRQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPMVKAKDERRERRDDFANETADDAEAGDSEEEEEE

Source Source

Source organism organism	Escherichia coli
NCBI taxonomy ID ncbi_taxonomy_id	562
Host organism host_organism	Escherichia coli

Entity 4 Entity 4 polymer

Description description	Small ribosomal subunit protein uS8
Molecular weight formula_weight	14146.6 kDa
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	H

MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEGFIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIAVVSTSKGVMTDRAARQAGLGGEIICYVA

Source Source

Source organism organism	Escherichia coli
NCBI taxonomy ID ncbi_taxonomy_id	562
Host organism host_organism	Escherichia coli

Entity 5 Entity 5 polymer

Description description	Translation initiation factor IF-1
Molecular weight formula_weight	8262.6 kDa
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	I

MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNYIRILTGDKVTVELTPYDLSKGRIVFRSR

Source Source

Source organism organism	Escherichia coli
NCBI taxonomy ID ncbi_taxonomy_id	562
Host organism host_organism	Escherichia coli

Entity 6 Entity 6 polymer

Description description	Translation initiation factor IF-3
Molecular weight formula_weight	20601.0 kDa
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	J

MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALEKAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGTDEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVESFPTKIEGRQMIMVLAPKKKQ

Source Source

Source organism organism	Escherichia coli
NCBI taxonomy ID ncbi_taxonomy_id	562
Host organism host_organism	Escherichia coli

Entity 7 Entity 7 polymer

Description description	Small ribosomal subunit protein uS11
Molecular weight formula_weight	15055.5 kDa
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	K

MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGWATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNAAGFRITNITDVTPIPHDGCRPPKKRRVGCRPPKKRRV

Source Source

Source organism organism	Escherichia coli
NCBI taxonomy ID ncbi_taxonomy_id	562
Host organism host_organism	Escherichia coli

Entity 8 Entity 8 polymer

Description description	Small ribosomal subunit protein uS12
Molecular weight formula_weight	13814.2 kDa
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	L

MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKKPNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKXLPGVRYHTVRGALDCSGVKDRKQARSKYGVKRPKA

Source Source

Source organism organism	Escherichia coli
NCBI taxonomy ID ncbi_taxonomy_id	562
Host organism host_organism	Escherichia coli

Entity 9 Entity 9 polymer

Description description	Small ribosomal subunit protein uS15
Molecular weight formula_weight	10290.8 kDa
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	O

MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFAEHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR

Source Source

Source organism organism	Escherichia coli
NCBI taxonomy ID ncbi_taxonomy_id	562
Host organism host_organism	Escherichia coli

Entity 10 Entity 10 polymer

Description description	Small ribosomal subunit protein bS16
Molecular weight formula_weight	9207.6 kDa
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	P

MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIASEKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA

Source Source

Source organism organism	Escherichia coli
NCBI taxonomy ID ncbi_taxonomy_id	562
Host organism host_organism	Escherichia coli

Entity 11 Entity 11 polymer

Description description	Small ribosomal subunit protein uS17
Molecular weight formula_weight	9724.5 kDa
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	Q

MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKLHVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL

Source Source

Source organism organism	Escherichia coli
NCBI taxonomy ID ncbi_taxonomy_id	562
Host organism host_organism	Escherichia coli

Entity 12 Entity 12 polymer

Description description	Small ribosomal subunit protein bS18
Molecular weight formula_weight	9005.5 kDa
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	R

MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRITGTRAKYQRQLARAIKRARYLSLLPYTDRHQ

Source Source

Source organism organism	Escherichia coli
NCBI taxonomy ID ncbi_taxonomy_id	562
Host organism host_organism	Escherichia coli

Entity 13 Entity 13 polymer

Description description	Small ribosomal subunit protein bS20
Molecular weight formula_weight	9708.5 kDa
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	T

MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDKAAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA

Source Source

Source organism organism	Escherichia coli
NCBI taxonomy ID ncbi_taxonomy_id	562
Host organism host_organism	Escherichia coli

Entity 14 Entity 14 polymer

Description description	Small ribosomal subunit protein bS21
Molecular weight formula_weight	8524.0 kDa
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	U

MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTERKRAKASAVKRHAKKLARENARRTRLY

Source Source

Source organism organism	Escherichia coli
NCBI taxonomy ID ncbi_taxonomy_id	562
Host organism host_organism	Escherichia coli

Entity 15 Entity 15 polymer

Description description	16S rRNA
Molecular weight formula_weight	499798.5 kDa
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polyribonucleotide
Chain IDs strand_id	B

AAAUUGAAGAGUUUGAUCAUGGCUCAGAUUGAACGCUGGCGGCAGGCCUAACACAUGCAAGUCGAACGGUAACAGGAAGAAGCUUGCUUCUUUGCUGACGAGUGGCGGACGGGUGAGUAAUGUCUGGGAAACUGCCUGAUGGAGGGGGAUAACUACUGGAAACGGUAGCUAAUACCGCAUAACGUCGCAAGACCAAAGAGGGGGACCUUCGGGCCUCUUGCCAUCGGAUGUGCCCAGAUGGGAUUAGCUAGUAGGUGGGGUAACGGCUCACCUAGGCGACGAUCCCUAGCUGGUCUGAGAGGAUGACCAGCCACACUGGAACUGAGACACGGUCCAGACUCCUACGGGAGGCAGCAGUGGGGAAUAUUGCACAAUGGGCGCAAGCCUGAUGCAGCCAUGCCGCGUGUAUGAAGAAGCCCUUCGGGUUGUAAAGUACUUUCAGCGGGGAGGAAGGGAGUAAAGUUAAUACCUUUGCUCAUUGACGUUACCCGCAGAAGAAGCACCGGCUAACUCCGUGCCAGCAGCCGCGGUAAUACGGAGGGUGCAAGCGUUAAUCGGAAUUACUGGGCGUAAAGCGCACGCAGGCGGUUUGUUAAGUCAGAUGUGAAAUCCCCGGGCUCAACCUGGGAACUGCAUCUGAUACUGGCAAGCUUGAGUCUCGUAGAGGGGGGUAGAAUUCCAGGUGUAGCGGUGAAAUGCGUAGAGAUCUGGAGGAAUACCGGUGGCGAAGGCGGCCCCCUGGACGAAGACUGACGCUCAGGUGCGAAAGCGUGGGGAGCAAACAGGAUUAGAUACCCUGGUAGUCCACGCCGUAAACGAUGUCGACUUGGAGGUUGUGCCCUUGAGGCGUGGCUUCCGGAGCUAACGCGUUAAGUCGACCGCCUGGGGAGUACGGCCGCAAGGUUAAAACUCAAAUGAAUUGACGGGGGCUUGUACACACCGUGGACCAUGUCGUUUCACACCAUGCAACGCGAAGAACCUUACCUGGUGUUGACAUCCAAAGAAGUUUUCAGAGAUGAGACUUAACCUUCGGGAACCGGGCGACAGUUACUGCAUGGCUGUUGUGAGUUCAUGUUGUGAACUGUUGGGUGAAGUCCCGUAACAAGCGUAACCCGUAUCCGGGGUAACCUGCGGUCCGGCCUGGAACUCAAAGGAGACUGCCAGUGAUAAACUGGAGGAAGGUGGGGAUGACGUCAAGUCAUCAUGGCCCUUACGACCAGGGCUACACACGUGCUACAAUGGCGCAUACAAAGAGAAGCGACCUCGCGAGAGCAAGCGGACCUCAUAAAGUGCGUCGUAGUCCGGAUUGGAGUCUGCAACUCGACUCCAUGAAGUCGGAAUCGCUAGUAAUCGUGGAUCAGAAUGCCACGGUGAAUACGUUCCCGGGCCUUGUACACACCGCCCGUCACACCAUGGGAGUGGGUUGCAAAAGAAGUAGGUAGCUUAACCUUCGGGAGGGCGCUUACCACUUUGUGAUUCAUGACUGGGGUGAAGUCGUAACAAGGUAACCGUAGGGGAACCUGCGGUUGGAUCACCUCCUUA

Source Source

Source organism organism	Escherichia coli
NCBI taxonomy ID ncbi_taxonomy_id	562
Host organism host_organism	Escherichia coli

Entity 16 Entity 16 non-polymer

Description description	POTASSIUM ION
Molecular weight formula_weight	39.1 kDa
Source method src_method	syn
Copy count entity_count	25

Entity 17 Entity 17 non-polymer

Description description	MAGNESIUM ION
Molecular weight formula_weight	24.3 kDa
Source method src_method	syn
Copy count entity_count	53

Entity 18 Entity 18 non-polymer

Description description	EDEINE B
Molecular weight formula_weight	781.9 kDa
Source method src_method	syn
Copy count entity_count	1

Entity 19 Entity 19 water

Description description	water
Molecular weight formula_weight	18.0 kDa
Source method src_method	nat
Copy count entity_count	1836

▶

Unit-cell length a length_a	1.000 Å
Unit-cell length b length_b	1.000 Å
Unit-cell length c length_c	1.000 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	—

Space group space_group	P 1
Space-group number int_tables_number	1
Crystal system crystal_system	—

Resolution resolution	2.000 Å
Low-resolution limit resolution_low	211.330 Å
R factor r_factor	0.3439
R_work r_work	0.3439
R_free r_free	—

Resolution resolution	2.00 Å
Resolution测定Method resolution_method	—
颗粒数 num_particles	642300

Structure of E. coli 30S-IF1-IF3-mRNA-Edeine complex

1. Protein Identity and Related Structures Protein Identity & Related Structures

Small ribosomal subunit protein uS4

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Small ribosomal subunit protein uS5

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Small ribosomal subunit protein bS6, fully modified isoform

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Small ribosomal subunit protein uS8

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Translation initiation factor IF-1

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Translation initiation factor IF-3

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Small ribosomal subunit protein uS11

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Small ribosomal subunit protein uS12

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Small ribosomal subunit protein uS15

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Small ribosomal subunit protein bS16

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer