9ss0

30S ribosomal subunit RimM-KO with IF1 and IF3 (State I)

Method: ELECTRON MICROSCOPY

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1. Protein Identity and Related Structures Protein Identity & Related Structures

Small ribosomal subunit protein uS2

OrganismNot specified

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 17 RNA 1	Small ribosomal subunit protein uS4 × 1 （P0A7V8） Small ribosomal subunit protein uS5 × 1 （P0A7W1） Small ribosomal subunit protein bS6, fully modified isoform × 1 （P02358） Small ribosomal subunit protein uS7 × 1 （P02359） Small ribosomal subunit protein uS8 × 1 （P0A7W7） Small ribosomal subunit protein uS9 × 1 （P0A7X3） Small ribosomal subunit protein uS11 × 1 （P0A7R9） Small ribosomal subunit protein uS12 × 1 （P0A7S3） Small ribosomal subunit protein uS15 × 1 （P0ADZ4） Small ribosomal subunit protein bS16 × 1 （P0A7T3） Small ribosomal subunit protein uS17 × 1 （P0AG63） Small ribosomal subunit protein bS18 × 1 （P0A7T7） Small ribosomal subunit protein bS20 × 1 （P0A7U7） Small ribosomal subunit protein bS21 × 1 （P68679） 16S Ribosomal RNA × 1 Translation initiation factor IF-1 × 1 （P69222） Translation initiation factor IF-3 × 1 （P0A707）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RS2_ECOLI
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain AA； PDBConstruct 1–241； UniProt 1–241

Small ribosomal subunit protein uS4

OrganismNot specified

UniProt P0A7V8

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 17 RNA 1	Small ribosomal subunit protein uS2 × 1 （P0A7V0） Small ribosomal subunit protein uS5 × 1 （P0A7W1） Small ribosomal subunit protein bS6, fully modified isoform × 1 （P02358） Small ribosomal subunit protein uS7 × 1 （P02359） Small ribosomal subunit protein uS8 × 1 （P0A7W7） Small ribosomal subunit protein uS9 × 1 （P0A7X3） Small ribosomal subunit protein uS11 × 1 （P0A7R9） Small ribosomal subunit protein uS12 × 1 （P0A7S3） Small ribosomal subunit protein uS15 × 1 （P0ADZ4） Small ribosomal subunit protein bS16 × 1 （P0A7T3） Small ribosomal subunit protein uS17 × 1 （P0AG63） Small ribosomal subunit protein bS18 × 1 （P0A7T7） Small ribosomal subunit protein bS20 × 1 （P0A7U7） Small ribosomal subunit protein bS21 × 1 （P68679） 16S Ribosomal RNA × 1 Translation initiation factor IF-1 × 1 （P69222） Translation initiation factor IF-3 × 1 （P0A707）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RS4_ECOLI
Isoform	—
PDB entities	2
Chains and sequence ranges	Author chain AC； PDBConstruct 1–206； UniProt 1–206

Small ribosomal subunit protein uS5

OrganismNot specified

UniProt P0A7W1

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 17 RNA 1	Small ribosomal subunit protein uS2 × 1 （P0A7V0） Small ribosomal subunit protein uS4 × 1 （P0A7V8） Small ribosomal subunit protein bS6, fully modified isoform × 1 （P02358） Small ribosomal subunit protein uS7 × 1 （P02359） Small ribosomal subunit protein uS8 × 1 （P0A7W7） Small ribosomal subunit protein uS9 × 1 （P0A7X3） Small ribosomal subunit protein uS11 × 1 （P0A7R9） Small ribosomal subunit protein uS12 × 1 （P0A7S3） Small ribosomal subunit protein uS15 × 1 （P0ADZ4） Small ribosomal subunit protein bS16 × 1 （P0A7T3） Small ribosomal subunit protein uS17 × 1 （P0AG63） Small ribosomal subunit protein bS18 × 1 （P0A7T7） Small ribosomal subunit protein bS20 × 1 （P0A7U7） Small ribosomal subunit protein bS21 × 1 （P68679） 16S Ribosomal RNA × 1 Translation initiation factor IF-1 × 1 （P69222） Translation initiation factor IF-3 × 1 （P0A707）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RS5_ECOLI
Isoform	—
PDB entities	3
Chains and sequence ranges	Author chain AD； PDBConstruct 1–167； UniProt 1–167

Small ribosomal subunit protein bS6, fully modified isoform

OrganismNot specified

UniProt P02358

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 17 RNA 1	Small ribosomal subunit protein uS2 × 1 （P0A7V0） Small ribosomal subunit protein uS4 × 1 （P0A7V8） Small ribosomal subunit protein uS5 × 1 （P0A7W1） Small ribosomal subunit protein uS7 × 1 （P02359） Small ribosomal subunit protein uS8 × 1 （P0A7W7） Small ribosomal subunit protein uS9 × 1 （P0A7X3） Small ribosomal subunit protein uS11 × 1 （P0A7R9） Small ribosomal subunit protein uS12 × 1 （P0A7S3） Small ribosomal subunit protein uS15 × 1 （P0ADZ4） Small ribosomal subunit protein bS16 × 1 （P0A7T3） Small ribosomal subunit protein uS17 × 1 （P0AG63） Small ribosomal subunit protein bS18 × 1 （P0A7T7） Small ribosomal subunit protein bS20 × 1 （P0A7U7） Small ribosomal subunit protein bS21 × 1 （P68679） 16S Ribosomal RNA × 1 Translation initiation factor IF-1 × 1 （P69222） Translation initiation factor IF-3 × 1 （P0A707）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RS6_ECOLI
Isoform	—
PDB entities	4
Chains and sequence ranges	Author chain AE； PDBConstruct 1–135； UniProt 1–135

Small ribosomal subunit protein uS7

OrganismNot specified

UniProt P02359

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 17 RNA 1	Small ribosomal subunit protein uS2 × 1 （P0A7V0） Small ribosomal subunit protein uS4 × 1 （P0A7V8） Small ribosomal subunit protein uS5 × 1 （P0A7W1） Small ribosomal subunit protein bS6, fully modified isoform × 1 （P02358） Small ribosomal subunit protein uS8 × 1 （P0A7W7） Small ribosomal subunit protein uS9 × 1 （P0A7X3） Small ribosomal subunit protein uS11 × 1 （P0A7R9） Small ribosomal subunit protein uS12 × 1 （P0A7S3） Small ribosomal subunit protein uS15 × 1 （P0ADZ4） Small ribosomal subunit protein bS16 × 1 （P0A7T3） Small ribosomal subunit protein uS17 × 1 （P0AG63） Small ribosomal subunit protein bS18 × 1 （P0A7T7） Small ribosomal subunit protein bS20 × 1 （P0A7U7） Small ribosomal subunit protein bS21 × 1 （P68679） 16S Ribosomal RNA × 1 Translation initiation factor IF-1 × 1 （P69222） Translation initiation factor IF-3 × 1 （P0A707）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RS7_ECOLI
Isoform	—
PDB entities	5
Chains and sequence ranges	Author chain AF； PDBConstruct 1–179； UniProt 1–179

Small ribosomal subunit protein uS8

OrganismNot specified

UniProt P0A7W7

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 17 RNA 1	Small ribosomal subunit protein uS2 × 1 （P0A7V0） Small ribosomal subunit protein uS4 × 1 （P0A7V8） Small ribosomal subunit protein uS5 × 1 （P0A7W1） Small ribosomal subunit protein bS6, fully modified isoform × 1 （P02358） Small ribosomal subunit protein uS7 × 1 （P02359） Small ribosomal subunit protein uS9 × 1 （P0A7X3） Small ribosomal subunit protein uS11 × 1 （P0A7R9） Small ribosomal subunit protein uS12 × 1 （P0A7S3） Small ribosomal subunit protein uS15 × 1 （P0ADZ4） Small ribosomal subunit protein bS16 × 1 （P0A7T3） Small ribosomal subunit protein uS17 × 1 （P0AG63） Small ribosomal subunit protein bS18 × 1 （P0A7T7） Small ribosomal subunit protein bS20 × 1 （P0A7U7） Small ribosomal subunit protein bS21 × 1 （P68679） 16S Ribosomal RNA × 1 Translation initiation factor IF-1 × 1 （P69222） Translation initiation factor IF-3 × 1 （P0A707）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RS8_ECOLI
Isoform	—
PDB entities	6
Chains and sequence ranges	Author chain AG； PDBConstruct 1–130； UniProt 1–130

Small ribosomal subunit protein uS9

OrganismNot specified

UniProt P0A7X3

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 17 RNA 1	Small ribosomal subunit protein uS2 × 1 （P0A7V0） Small ribosomal subunit protein uS4 × 1 （P0A7V8） Small ribosomal subunit protein uS5 × 1 （P0A7W1） Small ribosomal subunit protein bS6, fully modified isoform × 1 （P02358） Small ribosomal subunit protein uS7 × 1 （P02359） Small ribosomal subunit protein uS8 × 1 （P0A7W7） Small ribosomal subunit protein uS11 × 1 （P0A7R9） Small ribosomal subunit protein uS12 × 1 （P0A7S3） Small ribosomal subunit protein uS15 × 1 （P0ADZ4） Small ribosomal subunit protein bS16 × 1 （P0A7T3） Small ribosomal subunit protein uS17 × 1 （P0AG63） Small ribosomal subunit protein bS18 × 1 （P0A7T7） Small ribosomal subunit protein bS20 × 1 （P0A7U7） Small ribosomal subunit protein bS21 × 1 （P68679） 16S Ribosomal RNA × 1 Translation initiation factor IF-1 × 1 （P69222） Translation initiation factor IF-3 × 1 （P0A707）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RS9_ECOLI
Isoform	—
PDB entities	7
Chains and sequence ranges	Author chain AH； PDBConstruct 1–130； UniProt 1–130

Small ribosomal subunit protein uS11

OrganismNot specified

UniProt P0A7R9

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 17 RNA 1	Small ribosomal subunit protein uS2 × 1 （P0A7V0） Small ribosomal subunit protein uS4 × 1 （P0A7V8） Small ribosomal subunit protein uS5 × 1 （P0A7W1） Small ribosomal subunit protein bS6, fully modified isoform × 1 （P02358） Small ribosomal subunit protein uS7 × 1 （P02359） Small ribosomal subunit protein uS8 × 1 （P0A7W7） Small ribosomal subunit protein uS9 × 1 （P0A7X3） Small ribosomal subunit protein uS12 × 1 （P0A7S3） Small ribosomal subunit protein uS15 × 1 （P0ADZ4） Small ribosomal subunit protein bS16 × 1 （P0A7T3） Small ribosomal subunit protein uS17 × 1 （P0AG63） Small ribosomal subunit protein bS18 × 1 （P0A7T7） Small ribosomal subunit protein bS20 × 1 （P0A7U7） Small ribosomal subunit protein bS21 × 1 （P68679） 16S Ribosomal RNA × 1 Translation initiation factor IF-1 × 1 （P69222） Translation initiation factor IF-3 × 1 （P0A707）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RS11_ECOLI
Isoform	—
PDB entities	8
Chains and sequence ranges	Author chain AJ； PDBConstruct 1–129； UniProt 1–129

Small ribosomal subunit protein uS12

OrganismNot specified

UniProt P0A7S3

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 17 RNA 1	Small ribosomal subunit protein uS2 × 1 （P0A7V0） Small ribosomal subunit protein uS4 × 1 （P0A7V8） Small ribosomal subunit protein uS5 × 1 （P0A7W1） Small ribosomal subunit protein bS6, fully modified isoform × 1 （P02358） Small ribosomal subunit protein uS7 × 1 （P02359） Small ribosomal subunit protein uS8 × 1 （P0A7W7） Small ribosomal subunit protein uS9 × 1 （P0A7X3） Small ribosomal subunit protein uS11 × 1 （P0A7R9） Small ribosomal subunit protein uS15 × 1 （P0ADZ4） Small ribosomal subunit protein bS16 × 1 （P0A7T3） Small ribosomal subunit protein uS17 × 1 （P0AG63） Small ribosomal subunit protein bS18 × 1 （P0A7T7） Small ribosomal subunit protein bS20 × 1 （P0A7U7） Small ribosomal subunit protein bS21 × 1 （P68679） 16S Ribosomal RNA × 1 Translation initiation factor IF-1 × 1 （P69222） Translation initiation factor IF-3 × 1 （P0A707）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RS12_ECOLI
Isoform	—
PDB entities	9
Chains and sequence ranges	Author chain AK； PDBConstruct 1–124； UniProt 1–124

Small ribosomal subunit protein uS15

OrganismNot specified

UniProt P0ADZ4

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 17 RNA 1	Small ribosomal subunit protein uS2 × 1 （P0A7V0） Small ribosomal subunit protein uS4 × 1 （P0A7V8） Small ribosomal subunit protein uS5 × 1 （P0A7W1） Small ribosomal subunit protein bS6, fully modified isoform × 1 （P02358） Small ribosomal subunit protein uS7 × 1 （P02359） Small ribosomal subunit protein uS8 × 1 （P0A7W7） Small ribosomal subunit protein uS9 × 1 （P0A7X3） Small ribosomal subunit protein uS11 × 1 （P0A7R9） Small ribosomal subunit protein uS12 × 1 （P0A7S3） Small ribosomal subunit protein bS16 × 1 （P0A7T3） Small ribosomal subunit protein uS17 × 1 （P0AG63） Small ribosomal subunit protein bS18 × 1 （P0A7T7） Small ribosomal subunit protein bS20 × 1 （P0A7U7） Small ribosomal subunit protein bS21 × 1 （P68679） 16S Ribosomal RNA × 1 Translation initiation factor IF-1 × 1 （P69222） Translation initiation factor IF-3 × 1 （P0A707）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RS15_ECOLI
Isoform	—
PDB entities	10
Chains and sequence ranges	Author chain AN； PDBConstruct 1–89； UniProt 1–89

Small ribosomal subunit protein bS16

OrganismNot specified

UniProt P0A7T3

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 17 RNA 1	Small ribosomal subunit protein uS2 × 1 （P0A7V0） Small ribosomal subunit protein uS4 × 1 （P0A7V8） Small ribosomal subunit protein uS5 × 1 （P0A7W1） Small ribosomal subunit protein bS6, fully modified isoform × 1 （P02358） Small ribosomal subunit protein uS7 × 1 （P02359） Small ribosomal subunit protein uS8 × 1 （P0A7W7） Small ribosomal subunit protein uS9 × 1 （P0A7X3） Small ribosomal subunit protein uS11 × 1 （P0A7R9） Small ribosomal subunit protein uS12 × 1 （P0A7S3） Small ribosomal subunit protein uS15 × 1 （P0ADZ4） Small ribosomal subunit protein uS17 × 1 （P0AG63） Small ribosomal subunit protein bS18 × 1 （P0A7T7） Small ribosomal subunit protein bS20 × 1 （P0A7U7） Small ribosomal subunit protein bS21 × 1 （P68679） 16S Ribosomal RNA × 1 Translation initiation factor IF-1 × 1 （P69222） Translation initiation factor IF-3 × 1 （P0A707）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RS16_ECOLI
Isoform	—
PDB entities	11
Chains and sequence ranges	Author chain AO； PDBConstruct 1–82； UniProt 1–82

Small ribosomal subunit protein uS17

OrganismNot specified

UniProt P0AG63

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 17 RNA 1	Small ribosomal subunit protein uS2 × 1 （P0A7V0） Small ribosomal subunit protein uS4 × 1 （P0A7V8） Small ribosomal subunit protein uS5 × 1 （P0A7W1） Small ribosomal subunit protein bS6, fully modified isoform × 1 （P02358） Small ribosomal subunit protein uS7 × 1 （P02359） Small ribosomal subunit protein uS8 × 1 （P0A7W7） Small ribosomal subunit protein uS9 × 1 （P0A7X3） Small ribosomal subunit protein uS11 × 1 （P0A7R9） Small ribosomal subunit protein uS12 × 1 （P0A7S3） Small ribosomal subunit protein uS15 × 1 （P0ADZ4） Small ribosomal subunit protein bS16 × 1 （P0A7T3） Small ribosomal subunit protein bS18 × 1 （P0A7T7） Small ribosomal subunit protein bS20 × 1 （P0A7U7） Small ribosomal subunit protein bS21 × 1 （P68679） 16S Ribosomal RNA × 1 Translation initiation factor IF-1 × 1 （P69222） Translation initiation factor IF-3 × 1 （P0A707）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RS17_ECOLI
Isoform	—
PDB entities	12
Chains and sequence ranges	Author chain AP； PDBConstruct 1–84； UniProt 1–84

Small ribosomal subunit protein bS18

OrganismNot specified

UniProt P0A7T7

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 17 RNA 1	Small ribosomal subunit protein uS2 × 1 （P0A7V0） Small ribosomal subunit protein uS4 × 1 （P0A7V8） Small ribosomal subunit protein uS5 × 1 （P0A7W1） Small ribosomal subunit protein bS6, fully modified isoform × 1 （P02358） Small ribosomal subunit protein uS7 × 1 （P02359） Small ribosomal subunit protein uS8 × 1 （P0A7W7） Small ribosomal subunit protein uS9 × 1 （P0A7X3） Small ribosomal subunit protein uS11 × 1 （P0A7R9） Small ribosomal subunit protein uS12 × 1 （P0A7S3） Small ribosomal subunit protein uS15 × 1 （P0ADZ4） Small ribosomal subunit protein bS16 × 1 （P0A7T3） Small ribosomal subunit protein uS17 × 1 （P0AG63） Small ribosomal subunit protein bS20 × 1 （P0A7U7） Small ribosomal subunit protein bS21 × 1 （P68679） 16S Ribosomal RNA × 1 Translation initiation factor IF-1 × 1 （P69222） Translation initiation factor IF-3 × 1 （P0A707）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RS18_ECOLI
Isoform	—
PDB entities	13
Chains and sequence ranges	Author chain AQ； PDBConstruct 1–75； UniProt 1–75

Small ribosomal subunit protein bS20

OrganismNot specified

UniProt P0A7U7

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 17 RNA 1	Small ribosomal subunit protein uS2 × 1 （P0A7V0） Small ribosomal subunit protein uS4 × 1 （P0A7V8） Small ribosomal subunit protein uS5 × 1 （P0A7W1） Small ribosomal subunit protein bS6, fully modified isoform × 1 （P02358） Small ribosomal subunit protein uS7 × 1 （P02359） Small ribosomal subunit protein uS8 × 1 （P0A7W7） Small ribosomal subunit protein uS9 × 1 （P0A7X3） Small ribosomal subunit protein uS11 × 1 （P0A7R9） Small ribosomal subunit protein uS12 × 1 （P0A7S3） Small ribosomal subunit protein uS15 × 1 （P0ADZ4） Small ribosomal subunit protein bS16 × 1 （P0A7T3） Small ribosomal subunit protein uS17 × 1 （P0AG63） Small ribosomal subunit protein bS18 × 1 （P0A7T7） Small ribosomal subunit protein bS21 × 1 （P68679） 16S Ribosomal RNA × 1 Translation initiation factor IF-1 × 1 （P69222） Translation initiation factor IF-3 × 1 （P0A707）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RS20_ECOLI
Isoform	—
PDB entities	14
Chains and sequence ranges	Author chain AS； PDBConstruct 1–87； UniProt 1–87

Small ribosomal subunit protein bS21

OrganismNot specified

UniProt P68679

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 17 RNA 1	Small ribosomal subunit protein uS2 × 1 （P0A7V0） Small ribosomal subunit protein uS4 × 1 （P0A7V8） Small ribosomal subunit protein uS5 × 1 （P0A7W1） Small ribosomal subunit protein bS6, fully modified isoform × 1 （P02358） Small ribosomal subunit protein uS7 × 1 （P02359） Small ribosomal subunit protein uS8 × 1 （P0A7W7） Small ribosomal subunit protein uS9 × 1 （P0A7X3） Small ribosomal subunit protein uS11 × 1 （P0A7R9） Small ribosomal subunit protein uS12 × 1 （P0A7S3） Small ribosomal subunit protein uS15 × 1 （P0ADZ4） Small ribosomal subunit protein bS16 × 1 （P0A7T3） Small ribosomal subunit protein uS17 × 1 （P0AG63） Small ribosomal subunit protein bS18 × 1 （P0A7T7） Small ribosomal subunit protein bS20 × 1 （P0A7U7） 16S Ribosomal RNA × 1 Translation initiation factor IF-1 × 1 （P69222） Translation initiation factor IF-3 × 1 （P0A707）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RS21_ECOLI
Isoform	—
PDB entities	15
Chains and sequence ranges	Author chain AT； PDBConstruct 1–71； UniProt 1–71

Translation initiation factor IF-1

OrganismNot specified

UniProt P69222

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 17 RNA 1	Small ribosomal subunit protein uS2 × 1 （P0A7V0） Small ribosomal subunit protein uS4 × 1 （P0A7V8） Small ribosomal subunit protein uS5 × 1 （P0A7W1） Small ribosomal subunit protein bS6, fully modified isoform × 1 （P02358） Small ribosomal subunit protein uS7 × 1 （P02359） Small ribosomal subunit protein uS8 × 1 （P0A7W7） Small ribosomal subunit protein uS9 × 1 （P0A7X3） Small ribosomal subunit protein uS11 × 1 （P0A7R9） Small ribosomal subunit protein uS12 × 1 （P0A7S3） Small ribosomal subunit protein uS15 × 1 （P0ADZ4） Small ribosomal subunit protein bS16 × 1 （P0A7T3） Small ribosomal subunit protein uS17 × 1 （P0AG63） Small ribosomal subunit protein bS18 × 1 （P0A7T7） Small ribosomal subunit protein bS20 × 1 （P0A7U7） Small ribosomal subunit protein bS21 × 1 （P68679） 16S Ribosomal RNA × 1 Translation initiation factor IF-3 × 1 （P0A707）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	IF1_ECOLI
Isoform	—
PDB entities	17
Chains and sequence ranges	Author chain F1； PDBConstruct 1–72； UniProt 1–72

Translation initiation factor IF-3

OrganismNot specified

UniProt P0A707

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 17 RNA 1	Small ribosomal subunit protein uS2 × 1 （P0A7V0） Small ribosomal subunit protein uS4 × 1 （P0A7V8） Small ribosomal subunit protein uS5 × 1 （P0A7W1） Small ribosomal subunit protein bS6, fully modified isoform × 1 （P02358） Small ribosomal subunit protein uS7 × 1 （P02359） Small ribosomal subunit protein uS8 × 1 （P0A7W7） Small ribosomal subunit protein uS9 × 1 （P0A7X3） Small ribosomal subunit protein uS11 × 1 （P0A7R9） Small ribosomal subunit protein uS12 × 1 （P0A7S3） Small ribosomal subunit protein uS15 × 1 （P0ADZ4） Small ribosomal subunit protein bS16 × 1 （P0A7T3） Small ribosomal subunit protein uS17 × 1 （P0AG63） Small ribosomal subunit protein bS18 × 1 （P0A7T7） Small ribosomal subunit protein bS20 × 1 （P0A7U7） Small ribosomal subunit protein bS21 × 1 （P68679） 16S Ribosomal RNA × 1 Translation initiation factor IF-1 × 1 （P69222）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	IF3_ECOLI
Isoform	—
PDB entities	18
Chains and sequence ranges	Author chain F3； PDBConstruct 1–180； UniProt 1–180

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	9ss0
Deposition date deposition_date	2025-09-25
Structure title title	30S ribosomal subunit RimM-KO with IF1 and IF3 (State I)
Keywords keywords	Ribosome assembly, RimM, initiation factor IF1, initiation factor IF3, anti-association, RIBOSOME; RIBOSOME
Experimental Method method	ELECTRON MICROSCOPY

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

9ss0__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

9ss0__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

9ss0__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	66.56 Å
Rg (electron density)	66.52 Å
Total Rg	66.61 Å
Atom count	47149
Residues	3442
Excluded volume	722870 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`9ss0__assembly_1__model_1`	18-meric （18）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (18)

Entity 1 Entity 1 polymer

Description description	Small ribosomal subunit protein uS2
Molecular weight formula_weight	26781.7 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	AA
Atom count atom_count	47149

MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLEKTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLTNWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDALFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVAATVREGRSQDLASQAEESFVEAE

Entity 2 Entity 2 polymer

Description description	Small ribosomal subunit protein uS4
Molecular weight formula_weight	23514.2 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	AC

MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGARKPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVVYRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALELAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK

Entity 3 Entity 3 polymer

Description description	Small ribosomal subunit protein uS5
Molecular weight formula_weight	17629.4 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	AD

MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNGRVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEGTGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKSVEEILGK

Entity 4 Entity 4 polymer

Description description	Small ribosomal subunit protein bS6, fully modified isoform
Molecular weight formula_weight	15727.5 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	AE

MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGRRQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPMVKAKDERRERRDDFANETADDAEAGDSEEEEEE

Entity 5 Entity 5 polymer

Description description	Small ribosomal subunit protein uS7
Molecular weight formula_weight	20055.2 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	AF

MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVYSALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMRWIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRWLSLRSFSHQAGASSKQPALGYLN

Entity 6 Entity 6 polymer

Description description	Small ribosomal subunit protein uS8
Molecular weight formula_weight	14146.6 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	AG

MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEGFIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIAVVSTSKGVMTDRAARQAGLGGEIICYVA

Entity 7 Entity 7 polymer

Description description	Small ribosomal subunit protein uS9
Molecular weight formula_weight	14886.3 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	AH

MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETARMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAGFVTRDARQVERKKVGLRKARRRPQFSKR

Entity 8 Entity 8 polymer

Description description	Small ribosomal subunit protein uS11
Molecular weight formula_weight	13871.0 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	AJ

MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGWATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNAAGFRITNITDVTPIPHNGCRPPKKRRV

Entity 9 Entity 9 polymer

Description description	Small ribosomal subunit protein uS12
Molecular weight formula_weight	13768.2 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	AK

MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKKPNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGALDCSGVKDRKQARSKYGVKRPKA

Entity 10 Entity 10 polymer

Description description	Small ribosomal subunit protein uS15
Molecular weight formula_weight	10290.8 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	AN

MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFAEHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR

Entity 11 Entity 11 polymer

Description description	Small ribosomal subunit protein bS16
Molecular weight formula_weight	9207.6 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	AO

MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIASEKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA

Entity 12 Entity 12 polymer

Description description	Small ribosomal subunit protein uS17
Molecular weight formula_weight	9724.5 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	AP

MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKLHVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL

Entity 13 Entity 13 polymer

Description description	Small ribosomal subunit protein bS18
Molecular weight formula_weight	9005.5 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	AQ

MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRITGTRAKYQRQLARAIKRARYLSLLPYTDRHQ

Entity 14 Entity 14 polymer

Description description	Small ribosomal subunit protein bS20
Molecular weight formula_weight	9708.5 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	AS

MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDKAAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA

Entity 15 Entity 15 polymer

Description description	Small ribosomal subunit protein bS21
Molecular weight formula_weight	8524.0 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	AT

MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTERKRAKASAVKRHAKKLARENARRTRLY

Entity 16 Entity 16 polymer

Description description	16S Ribosomal RNA
Molecular weight formula_weight	499031.6 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polyribonucleotide
Chain IDs strand_id	D1

AAUUGAAGAGUUUGAUCAUGGCUCAGAUUGAACGCUGGCGGCAGGCCUAACACAUGCAAGUCGAACGGUAACAGGAAGAAGCUUGCUUCUUUGCUGACGAGUGGCGGACGGGUGAGUAAUGUCUGGGAAACUGCCUGAUGGAGGGGGAUAACUACUGGAAACGGUAGCUAAUACCGCAUAACGUCGCAAGACCAAAGAGGGGGACCUUCGGGCCUCUUGCCAUCGGAUGUGCCCAGAUGGGAUUAGCUAGUAGGUGGGGUAACGGCUCACCUAGGCGACGAUCCCUAGCUGGUCUGAGAGGAUGACCAGCCACACUGGAACUGAGACACGGUCCAGACUCCUACGGGAGGCAGCAGUGGGGAAUAUUGCACAAUGGGCGCAAGCCUGAUGCAGCCAUGCCGCGUGUAUGAAGAAGGCCUUCGGGUUGUAAAGUACUUUCAGCGGGGAGGAAGGGAGUAAAGUUAAUACCUUUGCUCAUUGACGUUACCCGCAGAAGAAGCACCGGCUAACUCCGUGCCAGCAGCCGCGGUAAUACGGAGGGUGCAAGCGUUAAUCGGAAUUACUGGGCGUAAAGCGCACGCAGGCGGUUUGUUAAGUCAGAUGUGAAAUCCCCGGGCUCAACCUGGGAACUGCAUCUGAUACUGGCAAGCUUGAGUCUCGUAGAGGGGGGUAGAAUUCCAGGUGUAGCGGUGAAAUGCGUAGAGAUCUGGAGGAAUACCGGUGGCGAAGGCGGCCCCCUGGACGAAGACUGACGCUCAGGUGCGAAAGCGUGGGGAGCAAACAGGAUUAGAUACCCUGGUAGUCCACGCCGUAAACGAUGUCGACUUGGAGGUUGUGCCCUUGAGGCGUGGCUUCCGGAGCUAACGCGUUAAGUCGACCGCCUGGGGAGUACGGCCGCAAGGUUAAAACUCAAAUGAAUUGACGGGGGCCCGCACAAGCGGUGGAGCAUGUGGUUUAAUUCGAUGCAACGCGAAGAACCUUACCUGGUCUUGACAUCCACGGAAGUUUUCAGAGAUGAGAAUGUGCCUUCGGGAACCGUGAGACAGGUGCUGCAUGGCUGUCGUCAGCUCGUGUUGUGAAAUGUUGGGUUAAGUCCCGCAACGAGCGCAACCCUUAUCCUUUGUUGCCAGCGGUCCGGCCGGGAACUCAAAGGAGACUGCCAGUGAUAAACUGGAGGAAGGUGGGGAUGACGUCAAGUCAUCAUGGCCCUUACGACCAGGGCUACACACGUGCUACAAUGGCGCAUACAAAGAGAAGCGACCUCGCGAGAGCAAGCGGACCUCAUAAAGUGCGUCGUAGUCCGGAUUGGAGUCUGCAACUCGACUCCAUGAAGUCGGAAUCGCUAGUAAUCGUGGAUCAGAAUGCCACGGUGAAUACGUUCCCGGGCCUUGUACACACCGCCCGUCACACCAUGGGAGUGGGUUGCAAAAGAAGUAGGUAGCUUAACCUUCGGGAGGGCGCUUACCACUUUGUGAUUCAUGACUGGGGUGAAGUCGUAACAAGGUAACCGUAGGGGAACCUGCGGUUGGAUCACCUCCUU

Entity 17 Entity 17 polymer

Description description	Translation initiation factor IF-1
Molecular weight formula_weight	8262.6 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	F1

MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNYIRILTGDKVTVELTPYDLSKGRIVFRSR

Entity 18 Entity 18 polymer

Description description	Translation initiation factor IF-3
Molecular weight formula_weight	20601.0 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	F3

MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALEKAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGTDEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVESFPTKIEGRQMIMVLAPKKKQ

▶

Unit-cell length a length_a	1.000 Å
Unit-cell length b length_b	1.000 Å
Unit-cell length c length_c	1.000 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	—

Space group space_group	P 1
Space-group number int_tables_number	1
Crystal system crystal_system	—

Resolution resolution	3.00 Å
Resolution测定Method resolution_method	—
颗粒数 num_particles	12314

30S ribosomal subunit RimM-KO with IF1 and IF3 (State I)

1. Protein Identity and Related Structures Protein Identity & Related Structures

Small ribosomal subunit protein uS2

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Small ribosomal subunit protein uS4

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Small ribosomal subunit protein uS5

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Small ribosomal subunit protein bS6, fully modified isoform

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Small ribosomal subunit protein uS7

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Small ribosomal subunit protein uS8

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Small ribosomal subunit protein uS9

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Small ribosomal subunit protein uS11

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Small ribosomal subunit protein uS12

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Small ribosomal subunit protein uS15

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer