9i78

Cryo-EM structure of Chaetomium thermophilum ribosome-bound SND3 translocon

Method: ELECTRON MICROSCOPY D_max: 196.3 Å Quality: EXCELLENT

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

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1. 结构基本信息 1. Structure Basics

条目编号 entry_id	9i78
沉积日期 deposition_date	2025-01-31
最后修订 last_revision	2025-11-12
结构标题 title	Cryo-EM structure of Chaetomium thermophilum ribosome-bound SND3 translocon
关键词 keywords	membrane protein biogenesis, SND3, ribosome, co-translation translocation, insertase; RIBOSOME
实验方法 method	ELECTRON MICROSCOPY

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2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	76.87 Å
回转半径 Rg (电子) rg_electron	78.59 Å
零角强度 I(0) i0	108727000000.00
分子量 molecular_weight	1974600.0 kDa
排除体积 excluded_volume	2125200 Å³
包络体积 envelope_volume	3387500 Å³
水化壳体积 shell_volume	323900 Å³
包络直径 envelope_diameter	268.1 Å
壳层 Rg shell_rg	93.79 Å
包络 Rg envelope_rg	79.02 Å
形状 Rg shape_rg	78.69 Å
总 Rg total_rg	78.44 Å
总原子数 total_atoms	133840
残基数 n_residues	10996
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

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3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	196.3 Å
Rg (实空间) rg_real	74.79 Å
Rg 误差 (实空间) rg_real_error	0.30 Å
I(0) (实空间) i0_real	1.0510e+11
I(0) 误差 (实空间) i0_real_error	1.6430e+09
Rg (倒空间) rg_reciprocal	77.76 Å
I(0) (倒空间) i0_reciprocal	109000000000.0000
解质量估计 total_estimate	0.9066
解质量评级 solution_quality	EXCELLENT a EXCELLENT solution
P(r) 峰数 n_peaks	2
主峰位置 r_peak_primary	88.3 Å
偏度 Skewness skewness	0.138
峰度 Kurtosis kurtosis	-0.563
角度范围 angular_range	— – 0.1000 Å⁻¹
当前正则化参数 α current_alpha	0.2278
最高正则化参数 α highest_alpha	12050000000.0000
实空间数据点数 n_real_points	21
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.000; Oscil: 0.967; Stabil: 0.983; Sysdev: 1.000; Positv: 1.000; Valcen: 0.995; Smooth: 0.000

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4. 晶体学与实验 4. Crystallography & Experiment

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5. 实体与聚合体信息 (51)

Entity 1 polymer

描述 description	Translocon Sec61/SecY plug domain-containing protein
分子量 formula_weight	52322.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	1
原子数 atom_count	133840

MSSLRFLDLVKPFVPFLPEVQQPESKIPFNQKLMWTGLTLLIFLVMSQMPLYGIVSSDSSDPLYWLRMMMASNRGTLMELGITPIISSGMVFQLLAGTHMIDVNLDLKSDRELYQSAQKLFALILSVGTATVYVFTGLYGPPSELGAGIVFLLILQLVLAGMIVILLDELLQKGYGLGSGISLFIATNICESIMWKAFSPTSINTGRGPEYEGAVIALFHLLMTWPNKQRALQEAFYRQNLPNIMNLLATIVVFAAVIYLQGFRVEIPVKSSRQRGARGSYPVRLFYTSNMPIMLQSTLSSNIFLISQMLYSRFSDNLLVRLFGVWEAKDGSSQLSAVSGLVYYMSPPLNFKEALLDPIHTFVYIAYMLTACAIFSKTWIEVSGSSPRDVAKQLKDQGLVMAGHRDQSMYKELKRVIPTAAAFGGACIGALSVASDLMGALGSGTGTLLAVTIIYGYFEIAAKEGDLAGMKGMIMG

Entity 2 polymer

描述 description	SEC61 beta subunit
分子量 formula_weight	12552.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	2

MASPRATSPAPAGEKGSATGASTSASPGVLGGPRGSSPPPPGGARTAIRRRAAADQKERLANQRPNSTRAAGAGGSSNTMLKLYTDESPGLKVDPVVVLVLSLVFIFSVVALHVIAKITRKFSS

Entity 3 polymer

描述 description	SEC61 gamma subunit
分子量 formula_weight	7818.2 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	3

MADQIQELLDVPREFLKDGIQFINRAQKPDRREFIKISQAVGVGFLIMGAVGYFVKLIHVPLNNILVGGA

Entity 4 polymer

描述 description	CCDC47
分子量 formula_weight	51723.9 kDa
来源方法 src_method	man
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	4

MANVLNNLFGGGKPSVSPDPLVAGDDFADFSQAAEPSPVTVQPLATTPSAVPLQPARPYTDYKDDDDKKWYRLHERYTWSDFKSEGIILCIISVFIILHVIGASLNRRKAKKWIRAHAAPLAAEFAVVGYSGIPKNVSDKKGEELVKALQDSNIAQGDNLIKERSLFEFATYATGRANVAFLEVKIALTKRFNPLTAFFESVLGFFFESGPEVGDRVEATLYPFDGKEADVVPDFPGAAELRSKDPKSTYDNFVWAIVHKECMKKARNDRYDLSLTYTKDHAKLPNWLAVMSESAEITDALLTPELIKAAEAAGDLFEYLVVTDQPEDKPKTLNETRPRKRVILKYRVPSNDDYTSLLPIFEYFLRMPDHLVQVAHFRPEVLRKVKVVRDEEIRKIQKAEEESKAEERAQEREKAKKAKRDQELSQLDAKAQKKYLEREREKELKRSMKKATIRA

来源 Source

来源生物 organism	Thermochaetoides thermophila DSM 1495
NCBI 分类编号 ncbi_taxonomy_id	759272
宿主生物 host_organism	Thermochaetoides thermophila DSM 1495

Entity 5 polymer

描述 description	Putative inorganic phosphate transporter protein
分子量 formula_weight	24180.2 kDa
来源方法 src_method	man
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	6

MALSPQITNLIIIFGMMQVSKKIPFDDPNVLNMCRAAYLASNLIILCISLYIKSVVDKKKDMTTLKYIEPAPPGSSEEGKLVTTTVHAYDVEQVKILIKSQLFGIGMMAFMHLYLKYTNPLLIQSIIPLKGALESNMAKIHLWGMPATGDLKRPFKQPGGLLGGLFGQSVQADKKAVEAAERAGRGGVKEEGSGSAWSHPQFEKGGGSGGGSGGSAWSHPQFEK

来源 Source

来源生物 organism	Thermochaetoides thermophila DSM 1495
NCBI 分类编号 ncbi_taxonomy_id	759272
宿主生物 host_organism	Thermochaetoides thermophila DSM 1495

Entity 6 polymer

描述 description	Translocon-associated protein subunit alpha
分子量 formula_weight	29964.1 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	7

MVNFKWSALALLALRVAAVFAQEESADAEPGVLKEVSPDELKVSIDTTFPDADIFGVKLVNGRPTKAIIEITNHEEVPVDVAFIGGILKTTKPLPEDAPHYASIVRNLTAVRYGVQVPAGEKHQLPYQFVLDMMPQDVNLVLSAVITNAAAGKIYEVEAHSGPASIVDPPTSIFDPQIIFLYLFLTAFFSGIAYWVYKTWLEQFFPQAKPSRAAGPAKGKKARRAGGATSDAEPVSGSESAGGNTSGAGEKEYDESWIPAHHINRPVARKVKGGSKSK

Entity 7 polymer

描述 description	60S ribosomal protein L2-like protein
分子量 formula_weight	27598.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	LA

MGRVIRNQRKGRGSVFTAHTRLRKAPAKFRPLDYAERHGYIRGIVKEIIHDPGRGAPLARVVFRSPYKYKQITETFIANEGMYTGQFIYAGKNAALTVGNILPLSSVPEGTVVSNVEEKPGDRGALGRTSGNYVTVVGHNPDEGKTRIKLPSGAKKVVPSSSRGMIGIVAGGGRTDKPLLKASRAKHKFAVKRNRWPKTRGVAMNPVDHPHGGGNHQHIGKASTISRYAAQGQKAGLIAARRTGLLRGTQKTKE

Entity 8 polymer

描述 description	60S ribosomal protein L3-like protein
分子量 formula_weight	44456.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	LB

MSHRKYEAPRHGSLAFLPRKRAARHRGRVKSFPKDDPKKPVHLTAAMGYKAGMTTIVRDLDRPGAKAHKKEVVEAVTIIDCPPMVVVGLVGYIETPRGLRSLTTVWAEHLSDEVKRRFYKNWYKSKKKAFTKYAKKYAENNGASITRELERIKKYCTVVRVLAHTQIRKTPLKQKKAHLMEIQINGGSVADKVEFGRSLFEKPVTIDTIFEKDEMIDVIAVTKGHGFVGVTARWGTKKLPRKTHKGLRKVACIGAWHPSHVQWTVARAGQMGYHHRTSVNHKIYRIGKGDDEANASTETDLTKKKITPMGGFVRYGEVNNDYVMIKGSVPGVKKRIMTLRKSLFTHTSRKALEKVELKWIDTSSEFGHGAFQTAAEKKQFMGTLKKDLQTSA

Entity 9 polymer

描述 description	60S ribosomal protein L4-like protein
分子量 formula_weight	39439.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	LC

MASRPTVTVFGADGKPTGATEVLPKVFSAPIRPDIVKHVHTGMAKNKRQPYAVSEKAGHQTSAESWGTGRAVARIPRVSGGGTHRAGQGAFGNMCRSGRMFAPTKIWRKWHVKINQGQKRFATASALAASAVAPLLMARGHQVSTVPEVPLVVDSAAVAGDAVAKTAAAYKLLKAIGAGPDVEKVKKSKKLRAGKGKMRGRRHRQRRGPLIVYSPEHDGKELVKGFRNIPGVETCPVDALNLLQLAPGGHLGRFIVWTSAAIKQLDAVYESKKGFFLPANIVSQADLSRLINSTEIQSVLRAPKGEARTKRACVQKKNPLRNKQIMLRLNPYASTFAKEKLGEVKAEEGKPPKVPASFKELLHEA

Entity 10 polymer

描述 description	60S ribosomal protein l5-like protein
分子量 formula_weight	35034.6 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	LD

MAFHKLVKNSAYYSRFQTKFKRRRQGKTDYYARKRLITQAKNKYNAPKYRLVVRFTNRDIITQMVTSEINGDKIFAAAYSHELRAYGINHGLTNWAAAYATGLLLARRVLAKLGLDKTFTGVEEPNGEYTLTEAAETEDGERRPFKAILDVGLARTSTGARVFGVMKGASDGGIFIPHSENRFPGYDIETEELDTEVLKKYIYGGHVAEYMETLADDDEERYKSQFVKYIEDDVEADSLEELYAEAHKQIRADPFRKYVSDAPKKSKEEWKAESLKYKKAKLSREERKARVEAKIKQLLAEQDE

Entity 11 polymer

描述 description	60S ribosomal protein L6
分子量 formula_weight	22133.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	LE

MSAAPTTKTFGKGTRTVPAPSEKAQKWYPAEDEAQPKKVRKAVRPWTPRKSLQPGTVLILLAGRFRGKRVVLLKCLDQGVLLVTGPFKINGVPLRRVNARYVIATSVKVDLTGVDQAKIDEVAQPKYFTAEKAKEKASEEAFFKQGEKPQKKPVSSTRAADQKAIDKALIANIKKVDMLASYLASSFSLRKGDKPHLMKF

Entity 12 polymer

描述 description	60S ribosomal protein l7-like protein
分子量 formula_weight	28821.8 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	LF

MSSTTVPTQNDILVPETLLKKRKSQEKARAERAAALEKRKQANKEKRQVIFKRAEKYVKEYREQEREKIRLARIAKQQGSFHIPAEAKLVFVIRIKGINKIPPKPRKILQLLRLRQINNGVFVKVTKATAEMIKIVEPWVAYGYPNLKSVRELIYKRGYGKVNGQRIPLTDNAIIEENLGKYGIICIEDLIHEIFTVGPNFKQAANFLWPFKLSNPNGGFRPRKFKHFIEGGDLGNREEHINALIRAMN

Entity 13 polymer

描述 description	60S ribosomal protein L8
分子量 formula_weight	29744.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	LG

MPPKSGKKVAPAPFPQGKAGAKKAPKNPLLEKRPRNFGIGQDIQPKRNLSRMVKWPEYIRLQRQKKILRMRLKVPPAIAQFQYTLDKNLAAQAFKLLNKYRPETKQEKKERLLREATAIKEGKKKEDVSKKPYTVKYGLNHVVGLIENKKASLVLIANDVDPIELVVFLPALCRKMGIPYAIIKGKARLGTLVHKKTAAVVAITEVRSEDKNELAKLISAVKEGYLEKVEDTRKRWGGGIMGFKAQKREEKRKKSLETAIKV

Entity 14 polymer

描述 description	60S ribosomal protein l9-like protein
分子量 formula_weight	21582.1 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	LH

MRYIHSEETIPVPENLKVSIKSRLVTVEGPRGKLQKDLSHIAVNFSVVKKGVIGLEIHHGSRKDVAALRTVRTIINNMIIGVTKGFKYKMRYVYAHFPINVNVEKNAETGCYEVEIRNFIGEKIVRKVVMAPGVEVEISKAQKDELILSGNSLEAVSQSAADIQQICKVRNKDIRKFLDGIYVSEKGNIVED

Entity 15 polymer

描述 description	60S ribosomal protein L10-like protein
分子量 formula_weight	25255.4 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	LI

MARRPARCYRYCKNKPYPKSRFNRGVPDPKIKIYDLGRKRATVDDFPLCVHLVSNEYEQLSSEALEAARICANKYLVKYAGKEGFHLRVRAHPYHVIRINKMLSCAGADRLQTGMRGAFGKPQGTVARVDIGQILISVRTRDSNRAIALEALRRCQYKFPGRQKIIVSKNWGFTPLRREEYLEKKAAGRVRVDGAYVQFLTNHGNLEENMRRFPDAFKA

Entity 16 polymer

描述 description	Putative ribosomal protein
分子量 formula_weight	20119.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	LJ

MSSEKAQNPMRELRIQKLVLNISVGESGDRLTRAAKVLEQLSGQTPVYSKARYTVRQFGIRRNEKIAVHVTVRGPKAEEILERGLKVKEYELRRRNFSETGNFGFGISEHIDLGIKYDPSIGIYGMDFYCCMTRPGERVAKRRRCKSRIGASHRITREETIRWFKQRFDGIVR

Entity 17 polymer

描述 description	60S ribosomal protein L13
分子量 formula_weight	24191.2 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	LL

MAIKHNQQIPNNHFRKHWQRRVRCHFDQPGKKVTRRLARRAKAAALAPRPVDKLRPIVRCPTVKYNRRTRLGRGFTLEELKAAGIPRLLAPTIGIAVDHRRKNLSEESLAANVQRLKDYKARLILFPRKSNKPKKADTPKDQQTAETTTSLRTSFGVEQPLAPGFTEISKSEIPAGIEGGAYRALRKARSDARLVGVREKRAKEKAEAEANKK

Entity 18 polymer

描述 description	60S ribosomal protein L14-like protein
分子量 formula_weight	16111.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	LM

MAEINVEATSWRRVEVGRVLKLENGSLAAIVEIIDHKRVLADGPSSDPKLATPRGIVPLSRALLTPIVIPKLPRGARTGAVKKAWEAYGVDAKWKETNWAKKQLQQERRQSLTDFDRFKVMRLKKQRRFEERKALAKIKAAA

Entity 19 polymer

描述 description	Ribosomal protein L15
分子量 formula_weight	24307.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	LN

MGALKYLEELSKKKQSDVVRFLLRVRCWEYRQLNVIHRASRPSRPDKARRLGYKAKQGYVIYRVRVRRGGRKRPVPKGATYGKPTNQGVNQLKYQRSLRATAEERVGRRCSNLRVLNSYWVNQDSTYKYYEVILVDPNHKAIRRDPRINWICNPVHKHRECRGLTSTGKKSRGLNKGHRFNKTRAGRRKTWKRHNTLSLWRYR

Entity 20 polymer

描述 description	60S ribosomal protein L16-like protein
分子量 formula_weight	22888.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	LO

XSSFESVVVIDGKGHLLGRLASIVAKQLLSGQKIVVVRCEALNISGEFFRAKLKYHAYLRKMTRYNPTRGGPFHFRAPSRIFYKAVRGMIPHKTARGAAALERLKVFEGVPPPYDKKKKMVVPQALRVLRLQPGRKYCTVGRLSHEVGWKYQDVVARLEERRKAKGAAYYERKKLAARQLSEAKKAAAAKVDPKVSEALAAYGY

Entity 21 polymer

描述 description	60S ribosomal protein l17-like protein
分子量 formula_weight	21072.2 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	LP

MVRYAATEIAPTKSARARGSYLRVSFKNTRETAQAINGWKLQRALTFLQNVIDKKEAVPMRRYAGSTGRTAQGKQWGVSRARWPVKSAQFLIGLLKNAEANADAKGLDTGNLIIKHIQVNQAPKQRRRTYRAHGRINPYMSNPCHIELILTEAEETVQKSEAVVRDVESHLSSRQRGVRIRRALTAA

Entity 22 polymer

描述 description	Ribosomal protein L18-like protein
分子量 formula_weight	24195.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	LQ

MDLVCAFIGPLFAPGARRLNLEEIAAESSSGIDLRWKHVRSSHRKAPKSDNVYLKLLVKLYRFLARRTEAPFNKVVLRRLFMSRINRPPVSLSRIARNLKNGNEKKTVVVVATVTDDNRLLQVPKMEVAALRFTAKARARIEAAGGRTLTLDQLALEKPTGANTLLLRGPKNAREAVKHFGFGPHKHKKPYVRSKGRKFERARGRRRSRGFKV

Entity 23 polymer

描述 description	Ribosomal protein L19
分子量 formula_weight	22409.2 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	LR

MVNLRTQKRLAASVLGCGEGKVWLDPNEVSEISNANSRQSIRKLVADGLIIKKPVTMHSRSRARELNLARRIGRHRGFGKRKGTADARMPQQVLWMRRQRVLRRLLVKYRASGKIDKHLYHELYHLAKGNTFKHKRALVEHIHRAKAEKARERQIKEEMDAKRARTKAARERKQERQAAKRNALLGEEEESK

Entity 24 polymer

描述 description	60S ribosomal protein L20
分子量 formula_weight	20341.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	LS

MGRLQEYQVIGRHLPTEANPNPALYRMRIFAPNEVVAKSRFWYFLRGLKKVKKATGEIVSVNVISEKHPLKVKNFGIWLRYDSRSGTHNMYKEYRETSRVAAVEAMYADMAARHRARFRSIHILKVVELEKTEDVKRPYIKQLVAKNLSFPLPHRVPKISTKKIFSAKRPSTFS

Entity 25 polymer

描述 description	60S ribosomal protein l21-like protein
分子量 formula_weight	18159.2 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	LT

MGHAAGLRSGTRYAFSRGFRKHGQIPLSTYLRTYRVGDIVDIKVNGAVQKGMPHKFYHGKTGVVYNVTKSAVGVIVYKRVKHRYIEKRINVRIEHVKPSRSREDFLRRVKENAELKKKAKAEGVPVQLKRQPAMPREAHTVSIADNKPVTLAPVAYETTI

Entity 26 polymer

描述 description	60S ribosomal protein L22-like protein
分子量 formula_weight	14302.6 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	LU

MAPVAKKSGAKGKGPKVTKKFIINASQPASDKIFDVSAFEKFLNERIKVDGRVGNLGDVIKISQIGDGKVEIIAHNDLSGRYLKYLTKKFLKKMQLRDWLRVVATSKGVYELKFYNIVNDEAEEEED

Entity 27 polymer

描述 description	60S ribosomal protein l23-like protein
分子量 formula_weight	14674.4 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	LV

MAKQKRGAPGGKLKMTLGLPVGAIMNCADNSGARNLYIIAVKGAGARLNRLPAGGVGDMVMATVKKGKPELRKKVHPAVIVRQAKPWKRFDGVFLYFEDNAGVIVNPKGEMKGSAITGPVGKEAAELWPRIASNAGVVM

Entity 28 polymer

描述 description	60S ribosomal protein L25-like protein
分子量 formula_weight	17175.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	LX

MAPKDVKKGGASKAAKGAQAKKAAQAALKGVHSHKKTKVRKSTTFHRPKTLVLSRAPKYPRKSIPHEPRLDEHKIIIHPLNTEGALKKIEEQNTLVFIVDVKANKAQIKQALKKLYDIDTVKINTLIRPDGTKKAFARLTPDVDALDIAATKLGLV

Entity 29 polymer

描述 description	60S ribosomal protein L26-like protein
分子量 formula_weight	15565.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	LY

MKVRPTVSSSRRKARKAHFSAPSSVRRVIMSAPLSKELREKYNVRSIPIRKGDEVQIVRGAHKDKEGKVTSVYRLKYVIHVERVTREKATGQTVPIGIHPSNVVITKLHLDKDRENILARIKAGREQVAKAKGKKTAA

Entity 30 polymer

描述 description	60S ribosomal protein L27
分子量 formula_weight	15803.7 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	LZ

MKFLKTSRVCLVTRGRYAGKKVVIIQPVDQGTKSHPYGHALVAGIERYPSKITRRMSKARIEKRSKIKPFIKVINYNHLMPTRYTLELEGLKAVINADTFKEPSQREEAKKTVKKVLEDRYNSGKNRWFFTPLRF

Entity 31 polymer

描述 description	60S ribosomal protein L28-like protein
分子量 formula_weight	16848.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	La

MPTRFSKTRKHRGHVSAGKGRVGKHRKHPGGRGMAGGQHHHRTNLDKYHPGYFGKVGMRHFHLLRNHYWRPSINIDKLWSLVPSDVREQYLSGQKKDTAPVIDLLSHGYAKLLGKGRLPEIPVVVRARYVSAEAERKVKEAGGVVELVA

Entity 32 polymer

描述 description	60S ribosomal protein L29
分子量 formula_weight	7529.7 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	Lb

MAKSKNSSQHNQSRKAHRNGIKKPKTQRYPSLKGTDPKFRRNHRHALHGTAKALKEFKEGKRETA

Entity 33 polymer

描述 description	60S ribosomal protein l30-like protein
分子量 formula_weight	11602.6 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	Lc

MAPKKSKSEAQSIGAKLALVIKSGKVVLGYRSTLKALRSGKAKLILIAANTPPLRKSELEYYSMMSKTPVHHYSGTNIELGTACGKLFRCSTMAILDAGDSDILADQQ

Entity 34 polymer

描述 description	Putative 60S ribosomal protein
分子量 formula_weight	13872.2 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	Ld

MSSTQKKQRSAIADVVAREYTIHLHKRLHGVTFKKRAPRAIKEIKKFAQKAMGTSDVRLDPQLNKKVWEQGIKGVPFRIRVRISRRRNDEEGAKEKLYSYVQAVNVKNPKGLLTSVVEEE

Entity 35 polymer

描述 description	60S ribosomal protein L32-like protein
分子量 formula_weight	15096.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	Le

MVAAKKHVPIVKKRTKRFMRHQSDRFKCLDSAWRKPKGIDNRVRRRFKGNLAMPSIGYGSNKKTKHMMPSGHKAFLVHNVKDVELLLMHNRTYAAEIAHNVSSRKRIDIITRAKQLGVKVTNAKAKVTTEV

Entity 36 polymer

描述 description	60S ribosomal protein l33-like protein
分子量 formula_weight	12330.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	Lf

MPSEAGHRLYVKGRHLSYQRGRRNTHPKTSLIKIEGVDDTAAANFYLGKRVAYVYRAQKEVRGTKIRVIWGKITRPHGNSGVVRAKFTHPLPARSFGASVRIMLYPSSI

Entity 37 polymer

描述 description	Ribosomal protein l34-like protein
分子量 formula_weight	13493.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	Lg

MAPTRVTYRRRNPYNTTSNRTRVIKTPGGQLRVLHIKKRGTAPKCGDCGIKLPGIPALRPREYATISKPKKTVQRAYGGSRCGNCVRDRIIRAFLIEEQKIVKKVLKEQSQAEKKASKK

Entity 38 polymer

描述 description	Dolichyl-diphosphooligosaccharide--protein glycotransferase
分子量 formula_weight	14643.4 kDa
EC 编号 ec_number	2.4.99.18
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	Lh

MSSNGKVKAGQLWSKNKEELTKILGELKTELSQLRIQKISSSGAKLNKIHDLRKSIARVLTVINAKQRAQLRLFYKNKKYLPLDLRPKLTRALRRRLSKEDASRVLEKTKKRLTHFPQRKYAVKAA

Entity 39 polymer

描述 description	60S ribosomal protein L36
分子量 formula_weight	12784.1 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	Li

MSEDATPKAPVERTGLIRGLNKGHKTTRRVLKERPSRRKGAQSKRTQFVRSLIREVAGLAPYERRVIELLRNGKDKRARKFSKKKLGTFGRAKRKCEELQRIIAESRRAH

Entity 40 polymer

描述 description	Ribosomal protein L37
分子量 formula_weight	10660.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	Lj

MTKGTSSFGKRHNKTHGLCRRCGRRSLHNQKKVCASCGYPAAKTRKYNWSEKAKRRKVTGTGRMRYLSTVPRRFKNGFRTGVPKGARGPAVSTSS

Entity 41 polymer

描述 description	60S ribosomal protein L38-like protein
分子量 formula_weight	9537.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	Lk

MPQEVSDIKKFIEICRRKDASSARIKKNPKTQQIKFKVRCQRFLYTLVLKDSDKAEKLKQSLPPNLQIKDVPKRNKRKSSA

Entity 42 polymer

描述 description	Ribosomal protein eL39
分子量 formula_weight	6297.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	Ll

MPSHKTFRIKQKLAKAQKQNRPIPQWIRLRTGNTIRYNAKRRHWRKTRLGL

Entity 43 polymer

描述 description	Putative ribosomal protein
分子量 formula_weight	14609.2 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	Lm

MQIFVKTLTGKTITLEVESSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGIIEPSLKALASKFNCDKMICRKCYARLPPRATNCRKRKCGHSNQLRPKKKLK

Entity 44 polymer

描述 description	60S ribosomal protein L41-A
分子量 formula_weight	3354.2 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	Ln

MRAKWRKKRTRRLKRKRRKVRARSK

Entity 45 polymer

描述 description	60S ribosomal protein L44-like protein
分子量 formula_weight	12045.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	Lo

MVNVPKTRKTFCKGKDCQKHTLHKVTQYKAGKASAFAQGKRRYDRKQSGYGGQTKPVFHKKAKTTKKIVLRLECSVCKTKKQLPLKRCKHFELGGDKKTKGAALVF

Entity 46 polymer

描述 description	60S ribosomal protein L43-like protein
分子量 formula_weight	10159.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	Lp

MSKRTKKVGISGKYGTRYGASLRKLVKKQEVTQHARYTCTFCGKNSVRRTAVGIWSCKSCKKTMAGGAYTVSTPAAAAMRSTLRRLREITEA

Entity 47 polymer

描述 description	Putative 60S ribosomal protein
分子量 formula_weight	15960.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	Lq

MSNVSADLIWEVSRNYNSFLVKQRTGTFSRDALNLTNQHSRKHAGFVNDKALGIVPAEKGVKVIAKKVKAANKPASSLYTVTYKSTARKAYKAIASQAAKHGYRADLRQAAVARASAILRSQRPVKPEPPKKLRGAAARRAAAAGKQ

Entity 48 polymer

描述 description	26S rRNA
分子量 formula_weight	1078730.1 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polyribonucleotide
链编号 strand_id	L1

AGGUUGACCUCGGAUCAGGUAGGAGGACCCGCUGAACUUAAGCAUAUCAAUAAGCGGAGGAAAAGAAACCAACAGGGAUUGCCCUAGUAACGGCGAGUGAAGCGGCAACAGCUCAAAUUUGAAAGCUGGCUUCGGCCCGCGUUGUAAUUUGGAGAGGAUGCUUUGGGCGAGGCUCCUUCUGAGUUCCCUGGAACGGGACGCCACAGAGGGUGAGAGCCCCGUAUAGUUGGAAGCCAAGCCUGUGUAAAGCUCCUUCGACGAGUCGAGUAGUUUGGGAAUGCUGCUCAAAAUGGGAGGUAAAUUUCUUCUAAAGCUAAAUACCGGCCAGAGACCGAUAGCGCACAAGUAGAGUGAUCGAAAGAUGAAAAGCACUUUGAAAAGAGGGUUAAAUAGCACGUGAAAUUGUUGAAAGGGAAGCGCUUGUGACCAGACUUGCGCCCGGCGGAUCAUCCGGUGUUCUCACCGGUGCACUCCGCCGGGCUCAGGCCAGCAUCGGUUCUGGCGGGGGGAUAAAGGCCCAGGGAAUGUGGCUCCUCCGGGAGUGUUAUAGCCCUGGGUGUAAUACCCUCGCCGGGACCGAGGACCGCGCUCUGCAAGGAUGCUGGCGUAAUGGUCACCAGCGACCCGUCUUGAAACACGGACCAAGGAGUCAAGGUUUUGCGCGAGUGUUUGGGUGUAAAACCCGCACGCGUAAUGAAAGUGAACGUAGGUGAGAGCUUCGGCGCAUCAUCGACCGAUCCUGAUGUAUUCGGAUGGAUUUGAGUAGGAGCGUUAAGCCUUGGACCCGAAAGAUGGUGAACUAUGCUUGGAUAGGGUGAAGCCAGAGGAAACUCUGGUGGAGGCUCGCAGCGGUUCUGACGUGCAAAUCGAUCGUCAAAUCUGAGCAUGGGGGCGAAAGACUAAUCGAACCAUCUAGUAGCUGGUUACCGCCGAAGUUUCCCUCAGGAUAGCAGUGUCGACCUUCAGUUUUAUGAGGUAAAGCGAAUGAUUAGGGACUCGGGGGCGAUUUUUAGCCUUCAUCCAUUCUCAAACUUUAAAUAUGUAAGAAGCCCUUGUUACUUAACUGAACGUGGGCAUUCGAAUGUAUCGACACUAGUGGGCCAUUUUUGGUAAGCAGAACUGGCGAUGCGGGAUGAACCGAACGCGGGGUUAAGGUGCCGGAGUGGACGCUCAUCAGACACCACAAAAGGCGUUAGUACAUCUUGACAGCAGGACGGUGGCCAUGGAAGUCGGAAUCCGCUAAGGACUGUGUAACAACUCACCUGCCGAAUGUACUAGCCCUGAAAAUGGAUGGCGCUCAAGCGUCCCACCCAUACCCCGCCCUCAGGGUAGAAACGAUGCCCUGAGGAGUAGGCGGCCGUGGAGGUCAGUGACGAAGCCUAGGGCGUGAGCCCGGGUCGAACGGCCUCUAGUGCAGAUCUUGGUGGUAGUAGCAAAUACUUCAAUGAGAACUUGAAGGACCGAAGUGGGGAAAGGUUCCAUGUGAACAGCGGUUGGACAUGGGUUAGUCGAUCCUAAGCCAUAGGGAAGUUCCGUUUCAAAGGGGCACUCGUGCCCCGUGUGGCGAAAGGGAAGCCGGUUAAUAUUCCGGCACCUGGAUGUGGGUUUUGCGCGGCAACGCAACUGAACGCGGAGACGACGGCGGGGGCCCCGGGCAGAGUUCUCUUUUCUUCUUAACGGUCUAUCACCCUGGAAACAGUUUGUCUGGAGAUAGGGUUUAAUGGCCGGAAGAGCCCGACACUUCUGUCGGGUCCGGUGCGCUCUCGACGUCCCUUGAAAAUCCGCGGGAGGGAAUAAUUCUCACGCCAGGUCGUACUCAUAACCGCAGCAGGUCCCCAAGGUGAACAGCCUCUGGUUGAUAGAACAAUGUAGAUAAGGGAAGUCGGCAAAAUAGAUCCGUAACUUCGGGAAAAGGAUUGGCUCUAAGGGUUGGGCACGUUGGGCUUUGGGCGGACGCCCUGGGAGCAGAGGGCCUCUAGCCGGGCAACCGGCCGGCGGCCCUCAGCACCCGGGGUUGAAGCCCUUAGCAGGCUUCGGCCGUCCGGCGUGCGGUUAACAACCAACUUAGAACUGGUACGGACAGGGGGAAUCUGACUGUCUAAUUAAAACAUAGCAUUGCGAUGGCCAGAAAGUGGUGUUGACGCAAUGUGAUUUCUGCCCAGUGCUCUGAAUGUCAAAGUGAAGAAAUUCAACCAAGCGCGGGUAAACGGCGGGAGUAACUAUGACUCUCUUAAGGUAGCCAAAUGCCUCGUCAUCUAAUUAGUGACGCGCAUGAAUGGAUUAACGAGAUUCCCACUGUCCCUAUCUACUAUCUAGCGAAACCACAGCCAAGGGAACGGGCUUGGCAAAAUCAGCGGGGAAAGAAGACCCUGUUGAGCUUGACUCUAGUUUGACAUUGUGAAAAGACAUAGGAGGUGUAGAAUAGGUGGGAGCUUCGGCGCCAGUGAAAUACCACUACUCCUAUUGUUUUUUUACUUAUUCAAUGAAGCGGGGCUGGACUUGCGUCCAACUUCUGGAGUUAAGGUCCUUCGCGGGCCGACCCGGGUUGAAGACAUUGUCAGGUGGGGAGUUUGGCUGGGGCGGCACAUCUGUUAAACCAUAACGCAGGUGUCCUAAGGGGGGCUCAUGGAGAACAGAAAUCUCCAGUAGAACAAAAGGGUAAAAGUCCCCUUGAUUUUGAUUUUCAGUGUGAAUACAAACCAUGAAAGUGUGGCCUAUCGAUCCUUUAGUCCCUCGAAAUUUGAGGCUAGAGGUGCCAGAAAAGUUACCACAGGGAUAACUGGCUUGUGGCGGCCAAGCGUUCAUAGCGACGUCGCUUUUUGAUCCUUCGAUGUCGGCUCUUCCUAUCAUACCGAAGCAGAAUUCGGUAAGCGUUGGAUUGUUCACCCACUAAUAGGGAACGUGAGCUGGGUUUAGACCGUCGUGAGACAGGUUAGUUUUACCCUACUGAUGAACUCGUCGCAAUGGUAAUUCAGCUUAGUACGAGAGGAACCGCUGAUUCAGAUAAUUGGUUUUUGCGGUUGUCCGACCGGGCAGUGCCGCGAAGCUACCAUCUGCUGGAUAAUGGCUGAACGCCUCUAAGUCAGAAUCCAUGCCAGAACGCGACGAUACUACCCGCACGUUGUAGACGUAUAAGAAUAGGCUCCGGCCUCGUAUCCUAGCAGGCGAUUCCUCCGCCGGCCUCGAAGUGGCCGUCGGUAAUUCGCGUAUUGCAAUUUAGACACGCGCGGGAUCAAAUCCUUUGCAGACGACUUAGAUGUGCGAAAGGGUCCUGUAAGCAGUAGAGUAGCCUUGUUGUUACGAUCUGCUGAGGGUAAGCCCUCCUUCGCCUAGAUUU

Entity 49 polymer

描述 description	5.8S rRNA
分子量 formula_weight	50147.7 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polyribonucleotide
链编号 strand_id	L2

AAACUUUCAACAACGGAUCUCUUGGUUCUGGCAUCGAUGAAGAACGCAGCGAAAUGCGAUAAGUAAUGUGAAUUGCAGAAUUCCGUGAAUCAUCGAAUCUUUGAACGCACAUUGCGCCCGCCGGUAUUCCGGCGGGCAUGCCUGUUCGAGCGUCAU

Entity 50 polymer

描述 description	5S rRNA
分子量 formula_weight	38596.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polyribonucleotide
链编号 strand_id	L3

ACAUACGACCAUACCUAGUGGAAAACUCGGGAUCCCGUCCGCUCUCCCAUAGAUAAGCCACUAAGGGCGGGGUUAGUACUCAGGUCGGUGACGACUGGGGAAUCCCCCGUGUUGUAUGUU

Entity 51 non-polymer

描述 description	MAGNESIUM ION
分子量 formula_weight	24.3 kDa
来源方法 src_method	syn
拷贝数 entity_count	379

▶

7. 引用文献 (1)

▶

晶胞边长 a length_a	1.000 Å
晶胞边长 b length_b	1.000 Å
晶胞边长 c length_c	1.000 Å
晶胞夹角 α angle_alpha	90.00 °
晶胞夹角 β angle_beta	90.00 °
晶胞夹角 γ angle_gamma	90.00 °
晶胞分子数 Z z_pdb	—

空间群 space_group	P 1
空间群编号 int_tables_number	1
晶系 crystal_system	triclinic

分辨率 resolution	2.20 Å
分辨率测定方法 resolution_method	—
颗粒数 num_particles	119533

fit	/app/data/saxs_computation/i7/9i78/9i78.fit
pddf	/app/data/pr_computation/i7/9i78/9i78.pr.out
profile	/app/data/saxs_computation/i7/9i78/9i78.dat

Cryo-EM structure of Chaetomium thermophilum ribosome-bound SND3 translocon

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

1. 结构基本信息 1. Structure Basics

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

4. 晶体学与实验 4. Crystallography & Experiment

晶胞参数 Unit Cell

对称性 Symmetry

冷冻电镜 EM 3D

5. 实体与聚合体信息 (51)

Entity 1 polymer

聚合体 Polymer

Entity 2 polymer

聚合体 Polymer

Entity 3 polymer

聚合体 Polymer

Entity 4 polymer

聚合体 Polymer

来源 Source

Entity 5 polymer

聚合体 Polymer

来源 Source

Entity 6 polymer

聚合体 Polymer

Entity 7 polymer

聚合体 Polymer

Entity 8 polymer

聚合体 Polymer

Entity 9 polymer

聚合体 Polymer

Entity 10 polymer

聚合体 Polymer

Entity 11 polymer

聚合体 Polymer

Entity 12 polymer

聚合体 Polymer

Entity 13 polymer

聚合体 Polymer

Entity 14 polymer

聚合体 Polymer

Entity 15 polymer

聚合体 Polymer

Entity 16 polymer

聚合体 Polymer

Entity 17 polymer

聚合体 Polymer

Entity 18 polymer

聚合体 Polymer

Entity 19 polymer

聚合体 Polymer

Entity 20 polymer

聚合体 Polymer

Entity 21 polymer

聚合体 Polymer

Entity 22 polymer

聚合体 Polymer

Entity 23 polymer

聚合体 Polymer

Entity 24 polymer

聚合体 Polymer

Entity 25 polymer

聚合体 Polymer

Entity 26 polymer

聚合体 Polymer

Entity 27 polymer

聚合体 Polymer

Entity 28 polymer

聚合体 Polymer

Entity 29 polymer

聚合体 Polymer

Entity 30 polymer

聚合体 Polymer

Entity 31 polymer

聚合体 Polymer

Entity 32 polymer

聚合体 Polymer

Entity 33 polymer

聚合体 Polymer

Entity 34 polymer