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9tyh

Structure of the MAP2K MEK1 in an active conformation in complex with its substrate MAPK ERK2

Method: ELECTRON MICROSCOPY D_max: 91.2 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9tyh
deposition_date	2026-01-19
title	Structure of the MAP2K MEK1 in an active conformation in complex with its substrate MAPK ERK2
keywords	protein kinases, phosphoryl transfer, MAPK, MAP2K, cancer signaling, SIGNALING PROTEIN; SIGNALING PROTEIN
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	28.69 Å
rg_electron	27.86 Å
i0	83114100.00
molecular_weight	72688.0 kDa
excluded_volume	91506 Å³
envelope_volume	112660 Å³
shell_volume	33746 Å³
envelope_diameter	91.6 Å
shell_rg	35.08 Å
envelope_rg	27.52 Å
shape_rg	27.87 Å
total_rg	28.56 Å
total_atoms	10217
n_residues	628
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	91.2 Å
rg_real	28.64 Å
rg_real_error	0.57 Å
i0_real	8.3110e+07
i0_real_error	1.2640e+06
rg_reciprocal	28.67 Å
i0_reciprocal	83120000.0000
total_estimate	0.8990
solution_quality	GOOD a GOOD solution
n_peaks	1
r_peak_primary	33.2 Å
skewness	0.263
kurtosis	-0.485
angular_range	— – 0.2750 Å⁻¹
current_alpha	0.0000
highest_alpha	26440000.0000
n_real_points	56
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.934; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.993; Smooth: 0.886

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4. Crystallography & Experiment

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5. Entities & Polymer Info (3)

Entity 1 polymer

description	Mitogen-activated protein kinase 1
formula_weight	41725.0 kDa
ec_number	2.7.11.24
mutation	Thr185Val
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	B
atom_count	10217

QGPMAAAAAAGAGPEMVRGQVFDVGPRYTNLSYIGEGAYGMVCSAYDNVNKVRVAIKKISPFEHQTYCQRTLREIKILLRFRHENIIGINDIIRAPTIEQMKDVYIVQDLMETDLYKLLKTQHLSNDHICYFLYQILRGLKYIHSANVLHRDLKPSNLLLNTTCDLKICDFGLARVADPDHDHTGFLVEYVATRWYRAPEIMLNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGILGSPSQEDLNCIINLKARNYLLSLPHKNKVPWNRLFPNADSKALDLLDKMLTFNPHKRIEVEQALAHPYLEQYYDPSDEPIAEAPFKFDMELDDLPKEKLKELIFEETARFQPGYRS

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Escherichia coli

Entity 2 polymer

description	;Dual specificity mitogen-activated protein kinase kinase 1,Uncharacterized protein,Dual specificity mitogen-activated protein kinase kinase 1 ;
formula_weight	44981.6 kDa
ec_number	2.7.12.2
mutation	residue 3-11 replaced with GRA24 KIM (15 residues),Ser218Asp,Ser222Asp
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A

MPLLERRGVSELPPLYINPAPDGSAVNGTSSAETNLEALQKKLEELELDEQQRKRLEAFLTQKQKVGELKDDDFEKISELGAGNGGVVFKVSHKPSGLVMARKLIHLEIKPAIRNQIIRELQVLHECNSPYIVGFYGAFYSDGEISICMEHMDGGSLDQVLKKAGRIPEQILGKVSIAVIKGLTYLREKHKIMHRDVKPSNILVNSRGEIKLCDFGVSGQLIDDMANDFVGTRSYMSPERLQGTHYSVQSDIWSMGLSLVEMAVGRYPIPPPDAKELELMFGCQVEGDAAETPPRPRTPGRPLSSYGMDSRPPMAIFELLDYIVNEPPPKLPSGVFSLEFQDFVNKCLIKNPAERADLKQLMVHAFIKRSDAEEVDFAGWLCSTIGLNQPSTPTHAAGVLEVLFQ

Source

organism	Toxoplasma gondii ME49
ncbi_taxonomy_id	508771
host_organism	Trichoplusia ni

Entity 3 non-polymer

description	ADENOSINE-5'-DIPHOSPHATE
formula_weight	427.2 kDa
src_method	syn
entity_count	2

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6. Citations (1)

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length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	triclinic

resolution	3.40 Å
resolution_method	—
num_particles	113564

fit	/app/data/saxs_computation/ty/9tyh/9tyh.fit
pddf	/app/data/pr_computation/ty/9tyh/9tyh.pr.out
profile	/app/data/saxs_computation/ty/9tyh/9tyh.dat

Structure of the MAP2K MEK1 in an active conformation in complex with its substrate MAPK ERK2

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

EM 3D

5. Entities & Polymer Info (3)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

Entity 3 non-polymer

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)