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9wmu

;Co-transcriptional histone H3K36 methylation complex containing RNA polymerase II elongation complex, Set2, and the upstream nucleosome. (temp130, type A) ;

Method: ELECTRON MICROSCOPY D_max: 261.3 Å Quality: EXCELLENT

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9wmu
deposition_date	2025-09-03
title	;Co-transcriptional histone H3K36 methylation complex containing RNA polymerase II elongation complex, Set2, and the upstream nucleosome. (temp130, type A) ;
keywords	chromatin, nucleosome, TRANSCRIPTION; TRANSCRIPTION
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	94.07 Å
rg_electron	92.30 Å
i0	21049600000.00
molecular_weight	1154800.0 kDa
excluded_volume	1412900 Å³
envelope_volume	2497700 Å³
shell_volume	235950 Å³
envelope_diameter	339.4 Å
shell_rg	82.30 Å
envelope_rg	92.35 Å
shape_rg	92.15 Å
total_rg	92.70 Å
total_atoms	80704
n_residues	9479
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	261.3 Å
rg_real	89.95 Å
rg_real_error	1.08 Å
i0_real	2.0200e+10
i0_real_error	4.1010e+08
rg_reciprocal	89.63 Å
i0_reciprocal	20750000000.0000
total_estimate	0.9125
solution_quality	EXCELLENT a EXCELLENT solution
n_peaks	1
r_peak_primary	92.2 Å
skewness	0.426
kurtosis	-0.589
angular_range	— – 0.0850 Å⁻¹
current_alpha	0.8503
highest_alpha	1544000000.0000
n_real_points	18
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.965; Stabil: 0.988; Sysdev: 1.000; Positv: 1.000; Valcen: 1.000; Smooth: 0.005

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4. Crystallography & Experiment

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5. Entities & Polymer Info (33)

Entity 1 polymer

description	DNA-directed RNA polymerase subunit
formula_weight	194107.4 kDa
ec_number	2.7.7.6
src_method	nat
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	80704

MSQFPYSSAPLRSVKEVQFGLLSPEEIRAISVVKIEYPEIMDESRQRPREGGLNDPKLGSIDRNFKCQTCGEGMAECPGHFGHMELAKPVFHIGFIPKIKKVCECICMNCGKLLLDETNPTMAQAIRIRDPKKRFNAVWQLCKTKMVCEADAPVDEYSEQKVVSRGGCGNTQPVVRKDGMKLWGTWKKSGFSDRDAQPERKLLTPGEILNVFKHISPEDCFRLGFNEDYARPEWMIITVLPVPPPQVRPSIAMDETTQGQDDLTHKLSDILKANINVQKLEMDGSPQHIINEVEQLLQFHVATYMDNDIAGQPQALQKSGRPVKAIRARLKGKEGRLRGNLMGKRVDFSARTVISGDPNLELDQVGVPISIAKTLSYPETVTQYNIHRLTEYVRNGPNEHPGAKYVIRDNGDRIDLRYHKRAGDIVLQYGWKVERHLMDDDPVLFNRQPSLHKMSMMAHRVKVMPYSTFRLNLSVTSPYNADFDGDEMNLHVPQSEETRAELSQLCAVPLQIVSPQSNKPVMGIVQDTLCGVRKMTLRDTFIEYEQVMNMLFWVPSWDGVVPQPAILKPKPLWTGKQLLSIAIPSGIHLQRTDGGNSLLSPKDNGMLIVDGKVMFGVVDKKTVGSGGGGLIHTVMREKGPKICAELFGNIQKVVNYWLLHNGFSIGIGDAIADASTMKEITHAISSAKEQVQEIIYKAQHNELELKPGMTLRESFEGEVSRTLNDARDSAGRSAEMNLKDLNNVKQMVSAGSKGSFINIAQMSACVGQQMVEGKRIAFGFADRSLPHFTKDDFSPESKGFVENSYLRGLTPQEFFFHAMAGREGLIDTAVKTAETGYIQRRLVKALEDIMVHYDGTTRNSLGDIIQFLYGEDGLDGTQVERQTIDTIPGSDKAFHKRYYVDLMDEKNSIKPDVIEYAADILGDVELQKELNSEYEQLVSDRKFLREIVFVNGDHNWPLPVNLRRIIQNAQQIFHLDRAKASDLTIPEIIHGVRDLCKKLFVLRGENELIKEAQQNATSLFQCLVRARLATRRILEEFRLNRDAFEWVLGTIEAQFQRSLVHPGEMVGVIAAQSIGEPATQMTLNTFHYAGVSSKNVTLGVPRLKEILNVAKNIKTPALTVYLDREIALDIEKAKVIQSSIEYTTLKNVTSATEIYYDPDPTSTVIEEDFDTVEAYFSIPDEKVEETIDKQSPWLLRLELDRARMLDKQLTMNQVADKISEVFSDDLFVMWSEDNADKLIIRCRVIRDPKAMDEELEAEEDQMLKRIEAHMLDLIALRGIPGISKVYMVKHKVSVPDESGEYKNEELWALETDGINLAEVMAVPGVDSSRTYSNSFVEILSVLGIEATRSSLYKEILNVIAFDGSYVNYRHMALLVDVMTSRGYLMAITRHGINRADTGALMRCSFEETVEILFEAGAAAELDDCRGVSENVMLGQLAPMGTGAFDVMIDEKLLTSLPADYAPTMPLFKGKATQGSATPYDNNAQYDDEFNHDDVADVMFSPMAETGSGDDRSGGLTEYAGIQSPYQPTSPGLSATSPGFAPTSPGFAPTSPRYSPTSPGYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPQYSPTSPQYSPTSPQYSPTSPQYSPTSPQYSPTSPQYSPTSPQYSPTSPQYSPTSPQYSPTSPQYSPTSPQYSPTSPQYSPTSPQYSPTSPQYSPASPQYSPSRHSPNGESKEGE

Entity 2 polymer

description	DNA-directed RNA polymerase subunit beta
formula_weight	139746.1 kDa
ec_number	2.7.7.6
src_method	nat
entity_count	1

Polymer

type	polypeptide(L)
strand_id	B

MSYDPYSIDDTITTEDCWTVISAFFEEKGLVSQQLDSFDEFMETSIQDLVWEEPRLILDQPAQHTNEKDNINKRYEIRFGKIYLSRPTMTEADGTTHAMFPQEARLRNLTYSSPVYLDMEKSMFTSIDDEGNPNATLDWQQVHEPIKDGVEEGNKVHIGKVPIMLRSKFCSLRTLDEVDLYKMKECPYDMGGYFVINGSEKVLIAQERSAANIVQVFKKAAPSPISHVAEIRSALEKGSRLISTMQIKLYGREDKGTGRTIKATLPYVKQDIPIVIVFRALGVVPDGEILQHICYDENDWQMLEMLKPCIEEGFVIQDKEVALDFIGRRGSAALGIRREKRIQYAKDILQKELLPHITQEEGFETRKTFFLGYMVNRLLLCALERKDQDDRDHFGKKRLDLAGPLLANLFRILFRKLTREIYRYMQRCIETDRDFNLNLAVKSTTITSGLKYSLATGNWGEQKKAMSSRAGVSQVLNRYTYSSTLSHLRRTNTPIGRDGKLAKPRQLHNTHWGLVCPAETPEGQACGLVKNLSLLSGISIGSPSEPIINFLEEWGMEPLEDYDPAQHTKSTRIFVNGVWTGIHRDPSMLVSTMRDLRRSGAISPEVSIIRDIREREFKIFTDVGRVYRPLFIVEDDESKDNKGELRITKEHIRKIQQGYDDDAMNDDSEEQEQDVYGWSSLVTSGVIEYVDGEEEETIMIAMTPEDLQTRSLEQKEIDLNDTAKRIKPEMSTSSHHTFTHCEIHPSMILGVAASIIPFPDHNQSPRNTYQSAMGKQAMGVFLTNYNVRMDTMANILYYPQKPLAKTQAMEYLKFRELPAGQNAIVAIACYSGYNQEDSMIMNQSSIDRGLFRSLFFRSYMDQEKRFGISIVEEFEKPTRATTLRLKHGTYEKLDEDGLIAPGVRVSGDDIIIGKTTPIPPDTEELGQRTKYHTKRDASTPLRSTENGIVDQVLLTTNQEGLKFVKVRMRTTKVPQIGDKFASRHGQKGTIGVTYRHEDMPFSAEGIVPDLIINPHAIPSRMTVAHLIECLLSKVGSIRGYEGDATPFTDLTVDAVSNLLRDNGYQSRGFEVMYNGHTGKKLMAQVFFGPTYYQRLRHMVDDKIHARARGPVQVLTRQPVEGRSRDGGLRFGEMERDCMIAHGAAGFLKERLMEASDAFRVHVCGICGLMSVIANLKKNQFECRSCKNKTNIYQLHIPYAAKLLFQELMAMNIAPRLYTERSGVSMRS

Entity 3 polymer

description	RNA polymerase II third largest subunit B44, part of central core
formula_weight	34216.3 kDa
src_method	nat
entity_count	1

Polymer

type	polypeptide(L)
strand_id	C

MSKEPKVNIINAQDDEVELMLSDVNLSLANSLRRTMLAEVPTLAIDLVEIKMNTSVLADEFISHRLGLIPLVSEDVEEMKYSRDCTCEDYCDECSVVLELSARHEGEEGTTDVYSSSLIKVSGPGNLNVGEPVRRDDYDQGILLCKLRNHQELNIRCIAKKGIAKEHAKWSPCSAIAFEYDPHNKLKHTDFWFEVDAKKEWPDSKYATWEEPPKPGEVFDYKAKPNRFYMTVETTGSLKANQVFSRGIKTLQEKLANVLFELENSRPANTTAYGGATAYGGQTVYGRETSYGGNTNYGDYNAPY

Entity 4 polymer

description	RNA polymerase II subunit B32
formula_weight	20623.0 kDa
src_method	nat
entity_count	1

Polymer

type	polypeptide(L)
strand_id	D

MNVSTSTVGARRRRAKQQVDDEENATLLRLGPEFALKQYDHDGNEHDLIALSLSESRLLIREALKARSRARNGGVDIESSNGEIDDDELAKVTSGAVANGVVKKTLDYLNTFARFKDEETCTAVDQLLHNSSDCSVLHPFEIAQLSSLGCEDVDEAITLIPSLAAKKEVNLQRILDELNRLEDPYK

Entity 5 polymer

description	DNA-directed RNA polymerases I, II, and III subunit RPABC1
formula_weight	24962.7 kDa
src_method	nat
entity_count	1

Polymer

type	polypeptide(L)
strand_id	E

MEDNNRIISRLWRSFRTVKEMAADRGYFISQEEMDQSLEEFRSKICDSMGNPQRKLMSFLANPTPEALEKYSDLGTLWVEFCDEPSVGIKTMRNFCLRIQEKNFSTGIFIYQNNITPSANKMIPTVSPAIIETFQESDLVVNITHHELVPKHIRLSDGEKSQLLQRYKLKESQLPRIQREDPVARYLGLKRGQVVKIIRRSETSGRYASYRICL

Entity 6 polymer

description	RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III
formula_weight	17803.6 kDa
src_method	nat
entity_count	1

Polymer

type	polypeptide(L)
strand_id	F

MSEDEAFNEQTENFENFEDEHFSDDNFEDRSTQPEDYAVGVTADGRQIINGDGIQEVNGTIKAHRKRSNKELAILKEERTTTPYLTKYERARILGTRALQISMNAPVLVDIEGETDPLQIAMKELSQRKIPLVIRRYLPDGSYEDWGCDELIVDN

Entity 7 polymer

description	RNA polymerase II subunit
formula_weight	18802.6 kDa
src_method	nat
entity_count	1

Polymer

type	polypeptide(L)
strand_id	G

MFFLKDLSLILTLHPSYFGPQMNQYLREKLLTDVEGTCTGQFGYIVTVLDGMNIDVGKGRIIPGSGSAEFEVKYRAVVWKPFKGEVVDAIVSNVSPIGFFADVGPLNVFVSTRLIPDNLVYNPSNSPPAYMSNDELITKGSKVRLKVVGTRTDVNEIYAIGSIKEDFLGAI

Entity 8 polymer

description	DNA-directed RNA polymerases I, II, and III subunit RPABC3
formula_weight	16249.2 kDa
src_method	nat
entity_count	1

Polymer

type	polypeptide(L)
strand_id	H

MSSALFDDIFTVQTVDNGRYNKVSRIIGISTTNSAIKLTLDINNEMFPVSQDDSLTVTLANSLSLDGEDESANFSKSWRPPKPTDKSLADDYDYVMFGTVYKFEEGDEDKIKVYVSFGGLLMCLEGGYKSLASLKQDNLYILIRR

Entity 9 polymer

description	DNA-directed RNA polymerase subunit
formula_weight	13612.3 kDa
src_method	nat
entity_count	1

Polymer

type	polypeptide(L)
strand_id	I

MASFRFCLECNNMLYPKEDKENQRLLYSCRNCDYTELAEDPKVYRHELITNIGETAGIVDDIGQDPTLPRSDKECPECHSRDCVFFQSQQRRKDTNMTLFYVCLNCKKTFRDESE

Entity 10 polymer

description	RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III
formula_weight	8554.1 kDa
src_method	nat
entity_count	1

Polymer

type	polypeptide(L)
strand_id	J

MIIPVRCFSCGKVVGDKWDAYLRLLEEGKQEGDALDELKLKRYCCRRMVLTHVDLIEKFLRYNPLEKKDFDS

Entity 11 polymer

description	RNA polymerase II subunit B12.5
formula_weight	13832.9 kDa
src_method	nat
entity_count	1

Polymer

type	polypeptide(L)
strand_id	K

MNAPDRFELFILPDDVPKLKITPDSRVPNCIIIKFEREDHTLANLLREELALYPDVTFVAYKVEHPLFANFVMRLQTEEGTRPKQALERACASIINKLKTLDHKFNEEWNIKNFSLND

Entity 12 polymer

description	RNA polymerase subunit ABC10-alpha
formula_weight	7862.0 kDa
src_method	nat
entity_count	1

Polymer

type	polypeptide(L)
strand_id	L

MSREGFVAPSGTDLAAAASGVAPNKHYGVKYTCGACAHNFSLNKSDPVRCKECGHRVIYKARTKRMIQFDAR

Entity 13 polymer

description	Transcription elongation factor 1 homolog
formula_weight	12606.9 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	M

GPGMGKRKSSARKPAPKIKQKLETQFTCLFCNHDNSVVCTLDKKNSIGLLECKKCNLSFQAPINSLSQPIDIYSDWIDACEAVAEENADVNGDNFIENDGADREQDDDYDDEF

Source

organism	Komagataella phaffii (strain GS115 / ATCC 20864)
ncbi_taxonomy_id	644223
host_organism	Escherichia coli

Entity 14 polymer

description	DNA (198-MER)
formula_weight	61251.7 kDa
src_method	syn
entity_count	1

Polymer

type	polydeoxyribonucleotide
strand_id	N

GCTTACGTCAGTCTGGCCATCTTTGTGTTTGGTGTGTTTGGGTGGTGGCCGTTTTCGTTGTTTTTTTCTGTCTCGTGCCTGGTGTCTTGGGTGTTTTCCCCTTGGCGGTTTTTTCGCGGGGGTCTGCGCGTTCGTGCGTTTTTGCGGTGCTTGTGCTGTCTTCGTCCTTTTGTGCGGCCTCGAAAACGGGATTCTGAT

Entity 15 polymer

description	;RNA (5'-R(PUPCPCPUPUPUPUPGPUPGPCPGPGPCPCPUPCPGP*A)-3') ;
formula_weight	5984.5 kDa
src_method	syn
entity_count	1

Polymer

type	polyribonucleotide
strand_id	P

UCCUUUUGUGCGGCCUCGA

Entity 16 polymer

description	DNA (198-MER)
formula_weight	60997.2 kDa
src_method	syn
entity_count	1

Polymer

type	polydeoxyribonucleotide
strand_id	T

ATCAGAATCCCGTTTTCGAGGCCGCACAAAAGGACGAAGACAGCACAAGCACCGCAAAAACGCACGAACGCGCAGACCCCCGCGAAAAAACCGCCAAGGGGAAAACACCCAAGACACCAGGCACGAGACAGAAAAAAACAACGAAAACGGCCACCACCCAAACACACCAAACACAAGAGCTAATTGACTGACGTAAGC

Entity 17 polymer

description	Transcription elongation factor SPT4
formula_weight	12039.6 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	V

MRERACMLCGIVLPGRVFMQNGCPNCDSVLNLRDSDQATVNECTSSSFEGLVAVGDNEHSWVAKWLRVDRFQPGLYAVRVDGRLPSDIVAALEQYGVYYRPRDGSVID

Source

organism	Komagataella phaffii (strain GS115 / ATCC 20864)
ncbi_taxonomy_id	644223
host_organism	Escherichia coli

Entity 18 polymer

description	Transcription elongation factor SPT5
formula_weight	101459.4 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	W

GPGMSETHKNQLDKVSTVSPDGPSEAVKEHSLQSKDLSKNDGQFIVPLDRNVIEQEEHKQVKSSAQAHNTTGDAADNEIEDGVPSEDVEFDKFKEDDYDEDDEVEEEGDIRSRKRRRHNQFLDVEAEVDDEEDDDDDDDDVELKHDFIQDDHIQHETQNEGFIAGHVDDDRLHRKLDQSREKIADQDAQELADEFKQRYGRSASSKYMGSASTTAPQRLLIPTVDDPGIWGVKVRLGKEKDVVRQILKKKLAREGTKNPLEIYSAFQRDSFKGHVYIEARKAEAINDALKGNVNVFSNNSKFLVGIVEYKDLLRPVKSSDVKLTRGSYVRVKNGKFKGDLAQVDEVLENGLEARLKLVPRLDYGKDLSHLSTSSSVDSTKNRRKFYTSKFRPAQRLFSEAEARVHEPTIRRDRDGFVTYGGEEYYEGFLYKTFRLQNLIVNSINPTLNELSLFQSNEESTTIDLSTIADSLKETAKNLVSFQPGDNVEIINGELNHLTGTVSSVNQSTIVSVRLHSDDDTINSETVEIPTSDLRKIFNVGDHVRVIHGKHTDDTGLIVEVNGDKVEFISNQTKRTVIVFSNYLIKSTDSTVSINESGRFELHDLVQVNSDLVGIVIRAQKDSFDVLCSDGKLLSLPPVSIYSKLNLNPNQQIAIDSNGVEVKVGDTVREFTGERRQGTILHVYRNFLFLRSREIVENQGVFVTSSNRVKTITSKSNGTGGQISGPDLSRMNPSRVIPPPSIPVANQRMTGRDPTLNKTVKIRQGGYKGKIGIVKEANGDRFRVELHNPNKTIPIPCSFLLIESTHGWVPYEDFVASDRRGGNIPRHEISGHVQQPQHGRAPAWGSGGKTPAWGSGGKTPAWGSGGSGGKTPAWGSGGKTPTWGSGAKTPAWGSGSKTPAWSGLDEEDRRDF

Source

organism	Komagataella phaffii (strain GS115 / ATCC 20864)
ncbi_taxonomy_id	644223
host_organism	Escherichia coli

Entity 19 polymer

description	Histone H3.3
formula_weight	15645.3 kDa
mutation	K36M
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	a,e

GSHMARTKQTARKSTGGKAPRKQLATKAARKSAPSTGGVMKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSAAIGALQEASEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Escherichia coli

Entity 20 polymer

description	Histone H4
formula_weight	11676.7 kDa
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	b,f

GSHMSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKVFLENVIRDAVTYTEHAKRKTVTAMDVVYALKRQGRTLYGFGG

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Escherichia coli

Entity 21 polymer

description	Histone H2A type 1-B/E
formula_weight	14447.8 kDa
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	c,g

GSHMSGRGKQGGKARAKAKTRSSRAGLQFPVGRVHRLLRKGNYSERVGAGAPVYLAAVLEYLTAEILELAGNAARDNKKTRIIPRHLQLAIRNDEELNKLLGRVTIAQGGVLPNIQAVLLPKKTESHHKAKGK

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Escherichia coli

Entity 22 polymer

description	Histone H2B type 1-J
formula_weight	14217.5 kDa
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	d,h

GSHMPEPAKSAPAPKKGSKKAVTKAQKKDGKKRKRSRKESYSIYVYKVLKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSAK

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Escherichia coli

Entity 23 polymer

description	Transcription elongation factor Spt6
formula_weight	173241.6 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	m

GPGMSAPSPSVSEEDINETRNSIDADASTVQDLLDQDAQEGSGSDNEGSNIVDSSEEDDDEDDEEEMQKVREGFIVDDDDENDEDGIPSSTKRKHRKKHKKRSAEVVEEVDEDDLQLLMENSGAVPGQQQNVKFKRLKRAEQDEKAQDSDSRGLNDMFSDEEGPGGVVEEGSEEEGLEDNLTTKTQRSGNLPHDEFDDFIEEDEFSDEDDEARDERLARMRSAKAAPQLAGLQGEQYQEIIEIFGDGTDYQWTLDAEEEMEQPQQDQEYDEAGEEIKGTTTSLADVFEPSELKEKMLTDEDNVIRVTDLPERFQAYRKSIKNYKLSDVDYSNERDWIVEQLKLEKRDFLQHLTQAHSSVAHLEEKFEASVKKIVDFIAIESFEVPFIWNHRRDYALHTYNDDSNNTIIVKLLNEDDLWRIVQLDLDYHSIHDKKAALSSIYKQLDLDVVDPTYEEFFGSARTLSELQDIDDYLTFNYSSQVKNLTAVAELSIEGNGSGEDEEQTTKSSFAEVKMKRKYSKYAIYDRIRQDAIYPVVQSIANISQMRENLAQSKRLHQVEDPIESPMDMIADIMSTEKDKTTFISSEKAYQAVKQFFSEQLSYEPFIRKTIRTAFQSFGVINIELTERGKLQIEPESPYFDFKYAKNRPISALTATPDLYLRMIQAENDGLVNIKVELPMLSTVVDHFYNILKSDGTSEISEKWNALRNDAWKQSLDKLIPLVQLNVKESIRRDCERVLYFQVKNSFTKKIDQAPYQPPTYAKGTIPRVLTLSFGEGNRGDAVLGVFMDDSGDVKSQIKFDEDFQSRDFSDSLTRYIKSNNINPDIIGISGFNIHTKKLFDKVNELVNEERLTIEYDNEYGYDREEDGRSDKHLIRVIYVNDETARLYQHSSKSSAEYPNRPQLAKYCIGLAKYIQSPLLEYLALDESMYSLHIHKHQNLLPREKLIDAVQTSIVDIVNLVGVDINEAVRAPYHALALPYVCGLGPRKAAGLIQSIQRIGSNLVNRAHLITEQLTSKTVFLNMASFVYIVFDPDVERNPQGEMDLLDSTRIHPEDYSLARKMAADALDIEDIDDDDESAMRNAIYEMVFPRSPPKDEDDLTFKLDELILDDYATELERKHQLKKRSTLQIIKEELQSRYREIRRDFHILNEAEIFQLLTRETVDSFRKGMVIPVYVRKVESSYMSVSTQSLIAGNIQRQDILEPNDRRDPREVYSVGQTVRACILDVDYYNFKCQLSLLRQFTENQVAGLNVNRNPKFWDIESENRDRQEEIDKQREESRESRVIKHPFFHNMKSKEAEDYLAARPVGDVVIRPSSKGSNHITISWKVAPQLYQHIDVLEENKDDANAIGRVLLVGKYRYHDLDELLVEYVNNVANKVELMVSHDKFMSDSLDYVKEWLERYSKANGNRSHYIFTFNRKAPGWFFLLFKLNPTSEIKIWNVKALPDGYLLANNVYPDTNSLCNGFKTLMSSRRQIKQRSNRAGGEYNNSHAGAYDNGYSNAPRY

Source

organism	Komagataella phaffii (strain GS115 / ATCC 20864)
ncbi_taxonomy_id	644223
host_organism	Spodoptera frugiperda

Entity 24 polymer

description	Protein that interacts with Spt6p and copurifies with Spt5p and RNA polymerase II
formula_weight	46907.8 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	n

GPGMSDEESENKKLLDQVLPEKTDQETQPEADAALAEQEAPENEPNEPASDDDSDSDLSDISDVDEEKVAEGLRYLDEQTAASLSKHKAAQPSSTHKKKEPTRRRPKQQTARETQYEPDVVEDEAARRTRLFEEKLDAAIKRKPNKRKKNDDVDLEQMQDELIQQLKLQMEESAIRDANNIEQGKPAIFKLKLLPKVKDILLRANLADSILDNNLLASVRLWLEPLPDASLPAYQIQKVLFDAIKSLPIKTSHLRESGLGKVMVFYQKSKRVEPNLKRTAEKLISDWTRPIMGASDNYKDMRVRTQQFDPAQFAESLPGRVSVRPQEAKTLYEEAAERRKRAAIPQARTAAYTIAPQVNTELLMSSARRTLPSGVGSSLSGEDQYKRLNSRLNTMGTKRKSSAKKGGISIEGRGLPQ

Source

organism	Komagataella phaffii (strain GS115 / ATCC 20864)
ncbi_taxonomy_id	644223
host_organism	Escherichia coli

Entity 25 polymer

description	Component of the Paf1p complex
formula_weight	124979.0 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	q

MKDHLIHNHHKHEHAHAEHDYKDDDDKEHLYFQGSSGSSGMSLLDQDYYLSALPEDKRAELVTLQLTIPLKSGDEVVTIDFNEDVDVSQLCVLLESENARPDLWLSAAKVFVSKGNIAGSQEIIRKALQSNVILDSSASVTSLFHNFSFWLSLMEYVSTNSREDQFLEYASNSLQASNSLDSGLILNGIGNGVLYAKSRRYDEALKEFDNLLKKKSTNILAILGKAQILYKRQKYSQALELYQKALTINPLIVPDPRLGIGLCFWHLNNKQLAEQAWHNSLKVHPQNNLNTKILICLAKFDYCFNESKDDDEFTALYRESLEFLHSCLKEDAKHPLLLMVLASYYFSKEDYEKVEKLCNLVLKENSRNAAFVSASHFWLARVAYHKEDYVQAQKQFKQAEDSQNSNTLAKLGYAQCLIARNEVGDATIYLEKFFKENQDSKSSEMMLLLGIIYSQSGKSYYKAIIFLEKYVAVCQEENYPILPEAYLVLSRVYENKDLNVALDYLMKANDILGDKANVYVLNNLGIYHFFRNNVSQSSDFFAQSLEALNNVSPQNKEALSITLHYNKARVEEVSNQSEAEKLYSKLMEKCPGYTSNKIRYIYLLALKSNGNNYADVQQLLDDFPSDLEVRSFYGWFLKRYGRKNGLKQDLESQHHKDTLINYDKHDCYALLSLGNIYATIAREMKVTDQKQNEIKRQQYLRAAQFYHKVLSIDPKNIYAAQGIAIIFSDKERTGLALEIFKKVRDTVQDLGTFINLGHCFMEAKQFGKAIESYTIALEKFSNGMDSKLLVLIGRAWYHRGFYEKSMDAYKKALEVSEQAYQLSKLPALRFNIVFIQFQIADFVKSLPNTQRDLTTLENALSGLNDAIKSLLKLAELEQPPYPSEDLKARATMGSNTLRNQLERAIQDQQDYEMSIQEKLRTARRKQQLDEEKRLEQEQRRLEEARKRQEAELIKRQELIKQAEEWNKMDIDAAKDNNDVLSEDGGEGEKKTTKKRKRKQKVSEDIAPDLDRINEENGNTEDYDPILDNEEEENYDEKDSENKENGQNGQNEEVYPESEGDDEDDVTNTKRQKRSKGIVDEEDSE

Source

organism	Komagataella phaffii (strain GS115 / ATCC 20864)
ncbi_taxonomy_id	644223
host_organism	Escherichia coli

Entity 26 polymer

description	RNAPII-associated chromatin remodeling Paf1 complex subunit
formula_weight	62301.2 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	r

MKDHLIHNHHKHEHAHAEHLYFQGSSGSSGMSDKEQDLDEDLLALAGAGSDSESNSAYSPEQEVSRKSKKIVNDSEDENNDSDGEQLLNPYPLEDKFKDEADRARLLGLSEMEREAILYERSQEVIKLKEKHLLSLRAKQSQLEKTAREGPKSSKTNKLSELKKQREQKSKRQRSDEYSDEEDDYRYEEVGDNDDVEMSDNYIDEDDAVFVEPKKSSRSQELSKPATLSDINKIIFGRTAMSKYWYYPEFDDVVKGMYLRLNTGSGGNGFSPYKVVEVLGSQRIKGSAYGLNSKENNCDMYLKVAFPNQKEMVRPLFVFSDSSITHPEFDLFLRELDAEGLSVMDLRDVDYKYHQLKEMSSRSLSNDEVNSIVKMKQSLSSNTGFNTVLKKAQLQEELEEARDAHDHERVARIEAELKSIGAESVVASKASSSMLKIDQRNKKLNNRFIRKAEMAAVEKRKLRGTSESDPFYRLATTARMFYKSANLGADDSNQPKTKAELEEEAKQEKKLEMQKLESMVKSNYRNGGLDRIISKIDFDFDLEL

Source

organism	Komagataella phaffii (strain GS115 / ATCC 20864)
ncbi_taxonomy_id	644223
host_organism	Escherichia coli

Entity 27 polymer

description	Histone-lysine N-methyltransferase, H3 lysine-36 specific
formula_weight	83724.5 kDa
ec_number	2.1.1.359
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	s

GPGMRERTVSDEEVDNYGHASHFKRKVQIYDYPADKTEEALTTFKTLSQNSYQNKTLGNAHNGRSTADGMSCDCEENWDTVSGINNACGEYSECINRLTSIECISGTCSCGDDCQNQRFQKKQYAPIAVFETEKKGYGVRAQADIRQDAFIYEYLGEVIDESTFRKRKENYDNQGLEHFYFMMLQKGEFIDATAKGGLGRFCNHSCRPNAYVDKWEVGNKLRMGIFAKREIYKGEEICFDYNVDRYGANPQKCYCGEDNCIGFLGGRTQTDSANILPVPIAEALGSTNAQEQRWVRLMKEQNKSIKASDYSSINEDYVNSLEMRPIADKDEASKVTSALLKTQDYIILRKLIERVSLTKDPEVLKEIVRLRGYQCVANILTMIVLDQEQQQVDVESFTLSVLDMLESWPNSSRNRISSSQIESVLESVKTKLKKIQPIGKKINSLLNSWSKLEISYKIPKSQSVGASNDVDNGHAESSTFQLRDEQKENNAASKISPFRIVDNMLLPEQWRWAVDESTGANYYYNKSQNITQWDLPPGCELLNIEVSEKNRDKKPPIDKSRLELKERQRKQEYERVKKEREREKLRLQREQEQEEQRRTETLKKIIANAEAKQQLLSKRLADEENEGKKRHKKSKRPTREQEWNKLFATVVPNMIKKHESQIGRDNLKHCAKDIVHQLSSKEIKKDTSKKPPKELSSEKKAKVKAFVGPYMEKYLDKWKAKHEKK

Source

organism	Komagataella phaffii (strain GS115 / ATCC 20864)
ncbi_taxonomy_id	644223
host_organism	Escherichia coli

Entity 28 polymer

description	Leo1
formula_weight	52387.7 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	u

MKDHLIHNHHKHEHAHAEHLYFQGSSGSSGMSQELEQNRHSGEESPDPVLDANTSDVDDDGLDLFGDDDDEQPEAKPGQLDDSSNDAEEDEQEKAGKPEAKVTSHTKSVYDNGETLDISLPIHPKSHIPQGKQDRWVVKLPDFLDINAEPFDPRPFEMNVKTHEDKNQELLDKLIAVNTVRWRYAKSETGGIFKETNSQIIQWEDGTYSLRVGSEIFDMFTTNTDDNYLVSEHNEEGILMTESTLSKSVKLVPASFQSTTHQKLAKALSAKQKKESYARSVVTKEDPEERQRRLESQENERYRLERRRKQAEEEEDYDSYAVSTSRSRPSRASQMYNAADEDDDDDDVAIGRTLASRRNNGYEDDGFIIDDEEEEEAVENDSADEESENEGDDDEEDDEEAAERLNRLKRAGASKYTNEGEQAQSEPVKSEEADNASQRDDAPRKKRRVILDDDEEEEE

Source

organism	Komagataella phaffii (strain GS115 / ATCC 20864)
ncbi_taxonomy_id	644223
host_organism	Escherichia coli

Entity 29 polymer

description	RNAP II-associated protein
formula_weight	46046.0 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	v

GSAMTSSKSRQDYIAKVRYQNDLPAPPCPPKLLKYEIEKEAPQKEFLKDSRLLSALFSKDNFRYLMNETSDGLDVNYLRIPGIIENEKSLGKLFSSYKNLAIENLHPDDRLLLVDPNKSATLNEESPVFFLRRPQYVSDGEKINLQNFTNKRKHQDMEDTNPRSQLHSVERTFDEVIDPRNKNRLQSLIHPRKKIKAVKAWHFFPDTSTFDQVFHSLKFVGSASLSKDRPLNEQLGQVSETDSTSVNASILTSLFKPIEINPHNKWISLYAVTDKLSAESFRKSFNSIKDDNIVNRHVIYDHIKDFDQMFRGHKKLFEDFAISFDDISDRAFFVPIVGRLELKKKRIVPGLVDMVNRTNYAHIRMDLRNPSTQETAIRDSRREQYDPVNYSSIQEE

Source

organism	Komagataella phaffii (strain GS115 / ATCC 20864)
ncbi_taxonomy_id	644223
host_organism	Escherichia coli

Entity 30 polymer

description	Constituent of Paf1 complex with RNA polymerase II, Paf1p, Hpr1p, Ctr9, Leo1, Rtf1 and Ccr4p
formula_weight	44760.7 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	x

MKDHLIHNHHKHEHAHAEHLYFQGSSGSSGMSEPLIALRQAIKDKKPVGVNEGETIGNAKVLNIGEKQYSLDAPTSFVINGKEFNLKVIYQCWVFRDSSSADYITECEKENIDGISFVERSELISWLKGEITSSAFIKGEKVGITEENGKNEGETKSNNKRKLSDDPLLKEIASNERVLIDHNKVLRGLKPKDFSSVAKDCELRILKEKPANAKSSDSNGRSSTSVSSSSKDARNKEPIIVLSPAASSLVRMSNVKEFLQEGKFLDPSKEPASSSNLLAIQRKSSRFKTPIKLLVVDNVEKLFTKSEYWDRVVAIVTTGKDWQFKNYKYKDPQILFQKFNGFYFKYKGDAVPASVKSWNVKVLDIDRVERFSDRQVVEQFWDTVENTLVAKRYKS

Source

organism	Komagataella phaffii (strain GS115 / ATCC 20864)
ncbi_taxonomy_id	644223
host_organism	Escherichia coli

Entity 31 non-polymer

description	ZINC ION
formula_weight	65.4 kDa
src_method	syn
entity_count	13

Entity 32 non-polymer

description	MAGNESIUM ION
formula_weight	24.3 kDa
src_method	syn
entity_count	1

Entity 33 non-polymer

description	S-ADENOSYL-L-HOMOCYSTEINE
formula_weight	384.4 kDa
src_method	syn
entity_count	1

▶

6. Citations (1)

▶

length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	—

resolution	4.28 Å
resolution_method	—
num_particles	15255

fit	/app/data/saxs_computation/wm/9wmu/9wmu.fit
pddf	/app/data/pr_computation/wm/9wmu/9wmu.pr.out
profile	/app/data/saxs_computation/wm/9wmu/9wmu.dat

;Co-transcriptional histone H3K36 methylation complex containing RNA polymerase II elongation complex, Set2, and the upstream nucleosome. (temp130, type A) ;

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

EM 3D

5. Entities & Polymer Info (33)

Entity 1 polymer

Polymer

Entity 2 polymer

Polymer

Entity 3 polymer

Polymer

Entity 4 polymer

Polymer

Entity 5 polymer

Polymer

Entity 6 polymer

Polymer

Entity 7 polymer

Polymer

Entity 8 polymer

Polymer

Entity 9 polymer

Polymer

Entity 10 polymer

Polymer

Entity 11 polymer

Polymer

Entity 12 polymer

Polymer

Entity 13 polymer

Polymer

Source

Entity 14 polymer

Polymer

Entity 15 polymer

Polymer

Entity 16 polymer

Polymer

Entity 17 polymer

Polymer

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Entity 18 polymer

Polymer

Source

Entity 19 polymer

Polymer

Source

Entity 20 polymer

Polymer

Source

Entity 21 polymer

Polymer

Source

Entity 22 polymer

Polymer

Source

Entity 23 polymer

Polymer

Source

Entity 24 polymer

Polymer

Source

Entity 25 polymer

Polymer

Source

Entity 26 polymer

Polymer

Source

Entity 27 polymer

Polymer

Source

Entity 28 polymer

Polymer