Current Protein Identity:G1SZ12 New Search
Main Difference Dimensions in This Set
Different construct Different assembly state Different ligand/ion Different experimental conditions Different structure-quality metrics

Difference tags compare only the current result set; every original PDB and assembly record remains separate.

Related-Structure Differences

Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.

PDB Entry Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Experimental Method Experimental Conditions Structure Quality
5LZS Structure of the mammalian ribosomal elongation complex with aminoacyl-tRNA, eEF1A, and didemnin B Deposited 2016-10-02 Assembly 1 Protein–RNA Heteromer;Protein × 78 PDB declaration: 86-meric(86) Consistent with all polymers
Chain M 1–218(218 aa)
Not recorded MG MAGNESIUM ION × 276 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 7C4 (2~{S})-~{N}-[(2~{R})-1-[[(3~{S},6~{S},8~{S},12~{S},13~{R},16~{S},17~{R},20~{S},23~{S})-13-[(2~{S})-butan-2-yl]-20-[(4-methoxyphenyl)methyl]-6,17,21-trimethyl-3-(2-methylpropyl)-12-oxidanyl-2,5,7,10,15,19,22-heptakis(oxidanylidene)-8-propan-2-yl-9,18-dioxa-1,4,14,21-tetrazabicyclo[21.3.0]hexacosan-16-yl]amino]-4-methyl-1-oxidanylidene-pentan-2-yl]-~{N}-methyl-1-[(2~{S})-2-oxidanylpropanoyl]pyrrolidine-2-carboxamide × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE;3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.31 Å
5LZT Structure of the mammalian ribosomal termination complex with eRF1 and eRF3. Deposited 2016-10-02 Assembly 1 Protein–RNA Heteromer;Protein × 80 PDB declaration: 87-meric(87) Consistent with all polymers
Chain M 1–218(218 aa)
Not recorded MG MAGNESIUM ION × 282 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE;3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.65 Å
5LZU Structure of the mammalian ribosomal termination complex with accommodated eRF1 Deposited 2016-10-02 Assembly 1 Protein–RNA Heteromer;Protein × 78 PDB declaration: 85-meric(85) Consistent with all polymers
Chain M 1–218(218 aa)
Not recorded MG MAGNESIUM ION × 258 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE;3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.75 Å
5LZV Structure of the mammalian ribosomal termination complex with accommodated eRF1(AAQ) and ABCE1. Deposited 2016-10-02 Assembly 1 Protein–RNA Heteromer;Protein × 80 PDB declaration: 87-meric(87) Consistent with all polymers
Chain M 1–218(218 aa)
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE;3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.35 Å
5LZW Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a truncated mRNA. Deposited 2016-10-02 Assembly 1 Protein–RNA Heteromer;Protein × 80 PDB declaration: 87-meric(87) Consistent with all polymers
Chain M 1–218(218 aa)
Not recorded MG MAGNESIUM ION × 281 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE;3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.53 Å
5LZX Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a UGA stop codon. Deposited 2016-10-02 Assembly 1 Protein–RNA Heteromer;Protein × 80 PDB declaration: 87-meric(87) Consistent with all polymers
Chain M 1–218(218 aa)
Not recorded MG MAGNESIUM ION × 264 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE;3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.67 Å
5LZY Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a polyadenylated mRNA. Deposited 2016-10-02 Assembly 1 Protein–RNA Heteromer;Protein × 79 PDB declaration: 86-meric(86) Consistent with all polymers
Chain M 1–218(218 aa)
Not recorded MG MAGNESIUM ION × 236 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE;3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.99 Å
5LZZ Structure of the mammalian rescue complex with Pelota and Hbs1l (combined) Deposited 2016-10-02 Assembly 1 Protein–RNA Heteromer;Protein × 80 PDB declaration: 87-meric(87) Consistent with all polymers
Chain M 1–218(218 aa)
Not recorded MG MAGNESIUM ION × 264 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE;3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.47 Å
6D90 Mammalian 80S ribosome with a double translocated CrPV-IRES, P-site tRNA and eRF1. Deposited 2018-04-27 Assembly 1 Protein–RNA Heteromer;Protein × 79 PDB declaration: 85-meric(85) Consistent with all polymers
Chain M 1–218(218 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6D9J Mammalian 80S ribosome with a double translocated CrPV-IRES, P-sitetRNA and eRF1. Deposited 2018-04-30 Assembly 1 Protein–RNA Heteromer;Protein × 79 PDB declaration: 85-meric(85) Consistent with all polymers
Chain M 1–218(218 aa)
Not recorded ZN ZINC ION × 4 MG MAGNESIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6HCF Structure of the rabbit 80S ribosome stalled on globin mRNA at the stop codon Deposited 2018-08-14 Assembly 1 Protein–RNA Heteromer;Protein × 81 PDB declaration: 87-meric(87) Consistent with all polymers
Chain M3 1–218(218 aa)
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6HCJ Structure of the rabbit 80S ribosome on globin mRNA in the rotated state with A/P and P/E tRNAs Deposited 2018-08-15 Assembly 1 Protein–RNA Heteromer;Protein × 78 PDB declaration: 85-meric(85) Consistent with all polymers
Chain M3 1–218(218 aa)
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
6HCM Structure of the rabbit collided di-ribosome (stalled monosome) Deposited 2018-08-15 Assembly 1 Protein–RNA Heteromer;Protein × 81 PDB declaration: 87-meric(87) Consistent with all polymers
Chain M3 1–218(218 aa)
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.80 Å
6HCQ Structure of the rabbit collided di-ribosome (collided monosome) Deposited 2018-08-16 Assembly 1 Protein–RNA Heteromer;Protein × 78 PDB declaration: 85-meric(85) Consistent with all polymers
Chain M3 1–218(218 aa)
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.50 Å
6SGC Rabbit 80S ribosome stalled on a poly(A) tail Deposited 2019-08-03 Assembly 1 Protein–RNA Heteromer;Protein × 79 PDB declaration: 86-meric(86) Consistent with all polymers
Chain M2 1–218(218 aa)
Not recorded MG MAGNESIUM ION × 275 ZN ZINC ION × 8 SPD SPERMIDINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
6T59 Structure of rabbit 80S ribosome translating beta-tubulin in complex with tetratricopeptide protein 5 and nascent chain-associated complex Deposited 2019-10-15 Assembly 1 Protein–RNA Heteromer;Protein × 49 PDB declaration: 53-meric(53) Consistent with all polymers
Chain M3 1–218(218 aa)
Not recorded MG MAGNESIUM ION × 220 ZN ZINC ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.11 Å
7MDZ 80S rabbit ribosome stalled with benzamide-CHX Deposited 2021-04-06 Assembly 1 Protein–RNA Heteromer;Protein × 75 PDB declaration: 81-meric(81) Consistent with all polymers
Chain M 1–218(218 aa)
Not recorded MG MAGNESIUM ION × 277 ZN ZINC ION × 8 Z2V N-[(1R,3S,4R,5S)-3-{(1R)-2-[(2R,4r,6S)-2,6-dihydroxypiperidin-4-yl]-1-hydroxyethyl}-4-hydroxy-1,5-dimethylcyclohexyl]benzamide × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE;3 sec blot
Resolution 3.20 Å
7NWG Mammalian pre-termination 80S ribosome with Hybrid P/E- and A/P-site tRNA's bound by Blasticidin S. Deposited 2021-03-16 Assembly 1 Protein–RNA Heteromer;Protein × 79 PDB declaration: 86-meric(86) Consistent with all polymers
Chain M3 1–218(218 aa)
Not recorded MG MAGNESIUM ION × 299 BLS BLASTICIDIN S × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4;50 mM HEPES pH 7.4 100 mM KOAc 5 mM Mg(Oac)2 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE;30s sample incubation on grid followed by 1.1s blotting time.
Resolution 3.80 Å
7NWH Mammalian pre-termination 80S ribosome with eRF1 and eRF3 bound by Blasticidin S. Deposited 2021-03-16 Assembly 1 Protein–RNA Heteromer;Protein × 79 PDB declaration: 84-meric(84) Consistent with all polymers
Chain M 1–218(218 aa)
Not recorded ZN ZINC ION × 8 MG MAGNESIUM ION × 281 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 BLS BLASTICIDIN S × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4;50 mM HEPES pH 7.4 100 mM KOAc 5 mM Mg(Oac)2 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE;30s sample incubation on grid followed by 1.1s blotting time.
Resolution 4.10 Å
7NWI Mammalian pre-termination 80S ribosome with Empty-A site bound by Blasticidin S Deposited 2021-03-16 Assembly 1 Protein–RNA Heteromer;Protein × 78 PDB declaration: 84-meric(84) Consistent with all polymers
Chain M 1–218(218 aa)
Not recorded MG MAGNESIUM ION × 210 ZN ZINC ION × 5 BLS BLASTICIDIN S × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4;50 mM HEPES pH 7.4 100 mM KOAc 5 mM Mg(Oac)2 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE;30s sample incubation on grid followed by 1.1s blotting time.
Resolution 3.13 Å
7O7Y Rabbit 80S ribosome stalled close to the mutated SARS-CoV-2 slippery site by a pseudoknot (high resolution) Deposited 2021-04-14 Assembly 1 Protein–RNA Heteromer;Protein × 79 PDB declaration: 86-meric(86) Consistent with all polymers
Chain BM 1–218(218 aa)
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 330 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.20 Å
7O7Z Rabbit 80S ribosome stalled close to the mutated SARS-CoV-2 slippery site by a pseudoknot (classified for pseudoknot) Deposited 2021-04-14 Assembly 1 Protein–RNA Heteromer;Protein × 79 PDB declaration: 86-meric(86) Consistent with all polymers
Chain BM 1–218(218 aa)
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 329 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.40 Å
7O80 Rabbit 80S ribosome in complex with eRF1 and ABCE1 stalled at the STOP codon in the mutated SARS-CoV-2 slippery site Deposited 2021-04-14 Assembly 1 Protein–RNA Heteromer;Protein × 81 PDB declaration: 88-meric(88) Consistent with all polymers
Chain BM 1–218(218 aa)
Not recorded SPD SPERMIDINE × 1 MG MAGNESIUM ION × 355 UNX UNKNOWN LIGAND × 374 ZN ZINC ION × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.90 Å
7O81 Rabbit 80S ribosome colliding in another ribosome stalled by the SARS-CoV-2 pseudoknot Deposited 2021-04-14 Assembly 1 Protein–RNA Heteromer;Protein × 80 PDB declaration: 87-meric(87) Consistent with all polymers
Chain BM 1–218(218 aa)
Not recorded MG MAGNESIUM ION × 349 UNX UNKNOWN LIGAND × 266 ZN ZINC ION × 8 SPD SPERMIDINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.10 Å
7TM3 Structure of the rabbit 80S ribosome stalled on a 2-TMD Rhodopsin intermediate in complex with the multipass translocon Deposited 2022-01-19 Assembly 1 Protein–RNA Heteromer;Protein × 50 PDB declaration: 54-meric(54) Consistent with all polymers
Chain M 1–218(218 aa)
Not recorded MG MAGNESIUM ION × 220 ZN ZINC ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5;50 mM HEPES-KOH, pH 7.5, 200 mM KOAc, 5 mM Mg(OAc)2, 0.25% digitonin, 50 mM biotin
cryo-EM vitrification conditions Cryogen ETHANE;blot for 4 sec with Whatman filter papers at a blot force of -15 before plunging
Resolution 3.25 Å
7TUT Structure of the rabbit 80S ribosome stalled on a 4-TMD Rhodopsin intermediate in complex with the multipass translocon Deposited 2022-02-03 Assembly 1 Protein–RNA Heteromer;Protein × 52 PDB declaration: 56-meric(56) Consistent with all polymers
Chain M 1–218(218 aa)
Not recorded MG MAGNESIUM ION × 220 ZN ZINC ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5;50 mM HEPES-KOH, pH 7.5, 200 mM potassium acetate, 5 mM magnesium acetate, 0.25% digitonin, 50 mM biotin
cryo-EM vitrification conditions Cryogen ETHANE;Blot for 4 seconds with Whatman filter papers at a blot force of -15 before plunging.
Resolution 3.88 Å
7ZJW Rabbit 80S ribosome as it decodes the Sec-UGA codon Deposited 2022-04-12 Assembly 1 Protein–RNA Heteromer;Protein × 79 PDB declaration: 86-meric(86) Consistent with all polymers
Chain LP 1–218(218 aa)
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 SER SERINE × 1 MG MAGNESIUM ION × 225 K POTASSIUM ION × 18 ZN ZINC ION × 9 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
7ZJX Rabbit 80S ribosome programmed with SECIS and SBP2 Deposited 2022-04-12 Assembly 1 Protein–RNA Heteromer;Protein × 77 PDB declaration: 83-meric(83) Consistent with all polymers
Chain LP 1–218(218 aa)
Not recorded ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8B5L Cryo-EM structure of ribosome-Sec61-TRAP (TRanslocon Associated Protein) translocon complex Deposited 2022-09-23 Assembly 1 Protein–RNA Heteromer;Protein × 48 PDB declaration: 51-meric(51) Consistent with all polymers
Chain M 2–139(138 aa)
Not recorded MG MAGNESIUM ION × 178 ZN ZINC ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.86 Å
8B6C Cryo-EM structure of ribosome-Sec61 in complex with cyclotriazadisulfonamide derivative CK147 Deposited 2022-09-26 Assembly 1 Protein–RNA Heteromer;Protein × 44 PDB declaration: 47-meric(47) Consistent with all polymers
Chain M 2–139(138 aa)
Not recorded MG MAGNESIUM ION × 178 ZN ZINC ION × 5 OWI 4-[[9-(cyclohexylmethyl)-3-methylidene-5-(4-methylphenyl)sulfonyl-1,5,9-triazacyclododec-1-yl]sulfonyl]-~{N},~{N}-dimethyl-aniline × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.79 Å
8BHF Cryo-EM structure of stalled rabbit 80S ribosomes in complex with human CCR4-NOT and CNOT4 Deposited 2022-10-31 Assembly 1 Protein–RNA Heteromer;Protein × 80 PDB declaration: 86-meric(86) Consistent with all polymers
Chain u3 2–139(138 aa)
Not recorded MG MAGNESIUM ION × 275 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8BPO Structure of rabbit 80S ribosome translating beta-tubulin in complex with tetratricopeptide protein 5 (TTC5) and S-phase Cyclin A Associated Protein residing in the ER (SCAPER) Deposited 2022-11-17 Assembly 1 Protein–RNA Heteromer;Protein × 48 PDB declaration: 51-meric(51) Consistent with all polymers
Chain L2 1–218(218 aa)
Not recorded MG MAGNESIUM ION × 214 ZN ZINC ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
8BTK Structure of the TRAP complex with the Sec translocon and a translating ribosome Deposited 2022-11-29 Assembly 1 Insufficient information Heteromer;Protein × 86 PDB declaration: 92-meric(92) Consistent with all polymers
Chain BM 1–218(218 aa)
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 750 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 AAC ACETYLAMINO-ACETIC ACID × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE;3.5 uL of sample was incubated on the grid for 60 seconds at 4*C and 100% humidity before being blotted and plunged into liquid ethane/propane mix cooled to liquid nitrogen temperature
Resolution 3.50 Å
8P2K Ternary complex of translating ribosome, NAC and METAP1 Deposited 2023-05-16 Assembly 1 Protein–RNA Heteromer;Protein × 82 PDB declaration: 88-meric(88) Consistent with all polymers
Chain BM 1–218(218 aa)
Not recorded N ANY 5'-MONOPHOSPHATE NUCLEOTIDE × 73 SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 312 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 AAC ACETYLAMINO-ACETIC ACID × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.90 Å
8RJB Structure of the rabbit 80S ribosome stalled on a 2-TMD rhodopsin intermediate in complex with Sec61-RAMP4 Deposited 2023-12-20 Assembly 1 Protein–RNA Heteromer;Protein × 47 PDB declaration: 51-meric(51) Consistent with all polymers
Chain M 1–218(218 aa)
Not recorded MG MAGNESIUM ION × 218 ZN ZINC ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.69 Å
8RJC Structure of the rabbit 80S ribosome stalled on a 2-TMD rhodopsin intermediate in complex with Sec61-TRAP, open conformation 1 Deposited 2023-12-20 Assembly 1 Protein–RNA Heteromer;Protein × 52 PDB declaration: 56-meric(56) Consistent with all polymers
Chain M 1–218(218 aa)
Not recorded MG MAGNESIUM ION × 218 ZN ZINC ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8RJD Structure of the rabbit 80S ribosome stalled on a 2-TMD rhodopsin intermediate in complex with Sec61-TRAP, open conformation 2 Deposited 2023-12-20 Assembly 1 Protein–RNA Heteromer;Protein × 52 PDB declaration: 56-meric(56) Consistent with all polymers
Chain M 1–218(218 aa)
Not recorded MG MAGNESIUM ION × 218 ZN ZINC ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.79 Å
8SCB Terminating ribosome with SRI-41315 Deposited 2023-04-05 Assembly 1 Protein–RNA Heteromer;Protein × 80 PDB declaration: 87-meric(87) Consistent with all polymers
Chain M 1–218(218 aa)
Not recorded MG MAGNESIUM ION × 312 ZN ZINC ION × 8 ZVM (2S,4aS)-2-cyclobutyl-10-methyl-3-phenyl-2,10-dihydropyrimido[4,5-b]quinoline-4,5(3H,4aH)-dione × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8VFT Translating 80S rabbit ribosome stalled by emetine with eEF2 Deposited 2023-12-22 Assembly 1 Protein–RNA Heteromer;Protein × 78 PDB declaration: 85-meric(85) Consistent with all polymers
Chain M 1–218(218 aa)
Not recorded MG MAGNESIUM ION × 276 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 34G emetine × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8VVP Codon sampling state obtained from Anisomycin-treated mammalian ribosomes Deposited 2024-01-31 Assembly 1 Protein–RNA Heteromer;Protein × 80 PDB declaration: 88-meric(88) Consistent with all polymers
Chain L 1–218(218 aa)
Not recorded MG MAGNESIUM ION × 260 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 6 SPM SPERMINE × 2 K POTASSIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5;20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8VVQ Codon sampling state of elongation inhibitor-treated mammalian ribosomes obtained from merged datasets Deposited 2024-01-31 Assembly 1 Protein–RNA Heteromer;Protein × 80 PDB declaration: 88-meric(88) Consistent with all polymers
Chain L 1–218(218 aa)
Not recorded MG MAGNESIUM ION × 290 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 K POTASSIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5;20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
8VVR Post-decoding/Post-hydrolysis state obtained from Anisomycin-treated mammalian ribosomes Deposited 2024-01-31 Assembly 1 Protein–RNA Heteromer;Protein × 80 PDB declaration: 88-meric(88) Consistent with all polymers
Chain L 1–218(218 aa)
Not recorded MG MAGNESIUM ION × 204 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 3 K POTASSIUM ION × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5;20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VVS Post-decoding post-hydrolysis state obtained from merged datasets of elongation inhibitor-treated mammalian ribosomes Deposited 2024-01-31 Assembly 1 Protein–RNA Heteromer;Protein × 80 PDB declaration: 88-meric(88) Consistent with all polymers
Chain L 1–218(218 aa)
Not recorded MG MAGNESIUM ION × 236 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 4 K POTASSIUM ION × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5;20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VVT Mammalian ribosomes bound to Anisomycin in the rotated conformation Deposited 2024-01-31 Assembly 1 Protein–RNA Heteromer;Protein × 80 PDB declaration: 84-meric(84) Consistent with all polymers
Chain L 1–218(218 aa)
Not recorded MG MAGNESIUM ION × 144 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5;20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8VVU Anisomycin-bound mammalian ribosome with partially accommodated A-site tRNA Deposited 2024-01-31 Assembly 1 Protein–RNA Heteromer;Protein × 78 PDB declaration: 86-meric(86) Consistent with all polymers
Chain L 1–218(218 aa)
Not recorded MG MAGNESIUM ION × 102 ZN ZINC ION × 8 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5;20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
8VVV Mammalian ribosomes bound to Anisomycin in the nonrotated conformation Deposited 2024-01-31 Assembly 1 Protein–RNA Heteromer;Protein × 79 PDB declaration: 83-meric(83) Consistent with all polymers
Chain M 1–218(218 aa)
Not recorded MG MAGNESIUM ION × 294 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5;20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9BDL 80S ribosome with angiogenin Deposited 2024-04-12 Assembly 1 Protein–RNA Heteromer;Protein × 75 PDB declaration: 82-meric(82) Consistent with all polymers
Chain AL14 2–139(138 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
9BDN 80S ribosome with angiogenin and tRNAAla Deposited 2024-04-12 Assembly 1 Protein–RNA Heteromer;Protein × 77 PDB declaration: 85-meric(85) Consistent with all polymers
Chain AL14 2–139(138 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9BDP 80S ribosome bound with angiogenin and complex of eEF1A and Ala-tRNAAla Deposited 2024-04-12 Assembly 1 Protein–RNA Heteromer;Protein × 78 PDB declaration: 86-meric(86) Consistent with all polymers
Chain AL14 2–139(138 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
9F1B Mammalian ternary complex of a translating 80S ribosome, NAC and NatA/E Deposited 2024-04-18 Assembly 1 Insufficient information Heteromer;Protein × 83 PDB declaration: 89-meric(89) Consistent with all polymers
Chain BM 1–218(218 aa)
Not recorded MG MAGNESIUM ION × 420 ZN ZINC ION × 8 UNX UNKNOWN LIGAND × 306 IHP INOSITOL HEXAKISPHOSPHATE × 1 SPD SPERMIDINE × 30 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.01 Å
9F1C Mammalian quaternary complex of a translating 80S ribosome, NAC, MetAP1 and NatA/E Deposited 2024-04-18 Assembly 1 Insufficient information Heteromer;Protein × 84 PDB declaration: 90-meric(90) Consistent with all polymers
Chain BM 1–218(218 aa)
Not recorded UNX UNKNOWN LIGAND × 277 MG MAGNESIUM ION × 420 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SPD SPERMIDINE × 30 SPM SPERMINE × 3 ZN ZINC ION × 8 IHP INOSITOL HEXAKISPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.78 Å
9F1D Mammalian quaternary complex of a translating 80S ribosome, NAC, MetAP1 and NatA/E-HYPK Deposited 2024-04-18 Assembly 1 Protein–RNA Heteromer;Protein × 85 PDB declaration: 91-meric(91) Consistent with all polymers
Chain BM 1–218(218 aa)
Not recorded UNX UNKNOWN LIGAND × 295 SPD SPERMIDINE × 30 MG MAGNESIUM ION × 420 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 IHP INOSITOL HEXAKISPHOSPHATE × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.26 Å
9MR4 NAC: Ribosome nascent chain complex(Oxa1L) Deposited 2025-01-06 Assembly 1 Protein–RNA Heteromer;Protein × 78 PDB declaration: 84-meric(84) Consistent with all polymers
Chain M 1–218(218 aa)
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 285 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.65 Å
9NDP Structure of stalled ribosome and nascent chain in complex with NMT2 and NAC Deposited 2025-02-18 Assembly 1 Insufficient information Heteromer;Protein × 81 PDB declaration: 87-meric(87) Consistent with all polymers
Chain M 1–218(218 aa)
Not recorded MG MAGNESIUM ION × 296 ZN ZINC ION × 6 COA COENZYME A × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.82 Å
9Q7Q ABCE1-eRF1-RNC-AMD1C Deposited 2025-08-25 Assembly 1 Protein–RNA Heteromer;Protein × 80 PDB declaration: 86-meric(86) Consistent with all polymers
Chain M 1–139(139 aa)
Not recorded MG MAGNESIUM ION × 291 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.86 Å
9QQA Ternary complex of translating ribosome, NAC and NMT1 Deposited 2025-03-31 Assembly 1 Protein–RNA Heteromer;Protein × 81 PDB declaration: 86-meric(86) Consistent with all polymers
Chain BM 1–218(218 aa)
Not recorded MG MAGNESIUM ION × 407 UNX UNKNOWN LIGAND × 187 SPD SPERMIDINE × 30 SPM SPERMINE × 3 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.80 Å
9QQB Quaternary complex of a translating ribosome, NAC, NMT1, and NatA Deposited 2025-03-31 Assembly 1 Protein–RNA Heteromer;Protein × 84 PDB declaration: 89-meric(89) Consistent with all polymers
Chain BM 1–218(218 aa)
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 412 UNX UNKNOWN LIGAND × 168 ZN ZINC ION × 8 IHP INOSITOL HEXAKISPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.43 Å
9RHU Rabbit 80S ribosome in complex with eRF1-AAQ, stalled at the Stop codon in mutated F2A sequence Deposited 2025-06-10 Assembly 1 Protein–RNA Heteromer;Protein × 81 PDB declaration: 88-meric(88) Consistent with all polymers
Chain I2 1–218(218 aa)
Not recorded MG MAGNESIUM ION × 367 ZN ZINC ION × 8 K POTASSIUM ION × 37 SPD SPERMIDINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.65 Å
9YPG GTPBP1*GCP*Phe-tRNA*ribosome in the GTPase activation-like state, Structure III Deposited 2025-10-14 Assembly 1 Protein–RNA Heteromer;Protein × 76 PDB declaration: 84-meric(84) Consistent with all polymers
Chain M 1–218(218 aa)
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPO GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIa Deposited 2025-10-14 Assembly 1 Protein–RNA Heteromer;Protein × 76 PDB declaration: 84-meric(84) Consistent with all polymers
Chain M 1–218(218 aa)
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 K POTASSIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPS GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIb Deposited 2025-10-14 Assembly 1 Protein–RNA Heteromer;Protein × 76 PDB declaration: 84-meric(84) Consistent with all polymers
Chain M 1–218(218 aa)
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPT GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIc Deposited 2025-10-14 Assembly 1 Protein–RNA Heteromer;Protein × 76 PDB declaration: 84-meric(84) Consistent with all polymers
Chain M 1–218(218 aa)
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 K POTASSIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9YPV GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IId Deposited 2025-10-14 Assembly 1 Protein–RNA Heteromer;Protein × 76 PDB declaration: 84-meric(84) Consistent with all polymers
Chain M 1–218(218 aa)
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPW GTPBP1*GDP*Phe-tRNA*ribosome in the post-GTP hydrolysis state, Structure IV Deposited 2025-10-14 Assembly 1 Protein–RNA Heteromer;Protein × 76 PDB declaration: 84-meric(84) Consistent with all polymers
Chain M 1–218(218 aa)
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YPY Ribosome with accommodated A-site tRNA, Structure V Deposited 2025-10-14 Assembly 1 Protein–RNA Heteromer;Protein × 75 PDB declaration: 83-meric(83) Consistent with all polymers
Chain M 1–218(218 aa)
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPZ Vacant ribosome with P-site tRNA, substate 1, Structure Ia Deposited 2025-10-14 Assembly 1 Protein–RNA Heteromer;Protein × 75 PDB declaration: 82-meric(82) Consistent with all polymers
Chain M 1–218(218 aa)
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YQ0 Vacant ribosome with P-site tRNA, substate 2, Structure Ib Deposited 2025-10-14 Assembly 1 Protein–RNA Heteromer;Protein × 75 PDB declaration: 82-meric(82) Consistent with all polymers
Chain M 1–218(218 aa)
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YQ1 Vacant ribosome with P-site tRNA, substate 3, Structure Ic Deposited 2025-10-14 Assembly 1 Protein–RNA Heteromer;Protein × 75 PDB declaration: 82-meric(82) Consistent with all polymers
Chain M 1–218(218 aa)
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å