Current Protein Identity：P06654
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1EM7 HELIX VARIANT OF THE B1 DOMAIN FROM STREPTOCOCCAL PROTEIN G Deposited 2000-03-16 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
189–244（56 aa）
Fragment：B1 DOMAIN
|
Mutation：A24E, K28R, Q32E, N35K, D36K | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 4.6；277 K；100 mM sodium acetate, 30% polyethylene glycol momomethyl Ether 2000, 200 mM ammonium sulfate, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
Resolution 2.00 Å R-free 0.267 |
| 1GB1 A NOVEL, HIGHLY STABLE FOLD OF THE IMMUNOGLOBULIN BINDING DOMAIN OF STREPTOCOCCAL PROTEIN G Deposited 1991-05-15 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
228–282（55 aa）
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR | mmCIF provides none of the parsed conditions | Resolution not provided |
| 1IGC IGG1 FAB FRAGMENT (MOPC21) COMPLEX WITH DOMAIN III OF PROTEIN G FROM STREPTOCOCCUS Deposited 1994-08-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
293–352（60 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.60 Å |
| 1IGD THE THIRD IGG-BINDING DOMAIN FROM STREPTOCOCCAL PROTEIN G: AN ANALYSIS BY X-RAY CRYSTALLOGRAPHY OF THE STRUCTURE ALONE AND IN A COMPLEX WITH FAB Deposited 1994-08-05 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
293–352（60 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 1.10 Å |
| 1LE3 NMR Structure of Tryptophan Zipper 4: A Stable Beta-Hairpin Peptide Based on the C-terminal Hairpin of the B1 Domain of Protein G Deposited 2002-04-09 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
267–282（16 aa）
Fragment：C-terminal hairpin of the B1 domain of Protein G
|
Mutation：Y45W/F52W/V54W Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 6；288 K；Ionic strength (raw mmCIF value) 0；Pressure ambient
NMR sample composition
2mM trpzip4 | 92% H2O, 8% D2O, pH 6.0, 0.1mM DSS
|
Resolution not provided |
| 1MPE Ensemble of 20 structures of the tetrameric mutant of the B1 domain of streptococcal protein G Deposited 2002-09-12 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
228–282（55 aa）
Fragment：B1 domain, sequence database residues 228-282
Chain B
228–282（55 aa）
Fragment：B1 domain, sequence database residues 228-282
Chain C
228–282（55 aa）
Fragment：B1 domain, sequence database residues 228-282
Chain D
228–282（55 aa）
Fragment：B1 domain, sequence database residues 228-282
|
Mutation：T2Q, L5V, A26F, F30V, Y33F, A34F Mutation：T2Q, L5V, A26F, F30V, Y33F, A34F Mutation：T2Q, L5V, A26F, F30V, Y33F, A34F Mutation：T2Q, L5V, A26F, F30V, Y33F, A34F | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 5.45；313 K；Ionic strength (raw mmCIF value) 50 mM sodium phosphate, 0.02% sodium azide；Pressure 1
NMR sample composition
3.5 mM (in monomer) U-15N,13C, 50 mM sodium phosphate buffer, 0.02% sodium azide | 90% H2O/10% D2O
NMR sample composition
2.89 mM (in monomer) U-15N, 50 mM sodium phosphate buffer, 0.02% sodium azide | 90% H2O/10% D2O
NMR sample composition
3.5 mM (in monomer) U-15N,13C, 50 mM sodium phosphate buffer, 0.02% sodium azide | 100% D2O
NMR sample composition
1.4 mM (in monomer) 1:1 mixture unlabelled:U-15N,13C, 50 mM sodium phosphate buffer, 0.02% sodium azide | 100% D2O
|
Resolution not provided |
| 1MVK X-ray structure of the tetrameric mutant of the B1 domain of streptococcal protein G Deposited 2002-09-25 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
228–282（55 aa）
Fragment：B1 domain, sequence database residues 228-282
Chain B
228–282（55 aa）
Fragment：B1 domain, sequence database residues 228-282
Chain C
228–282（55 aa）
Fragment：B1 domain, sequence database residues 228-282
Chain D
228–282（55 aa）
Fragment：B1 domain, sequence database residues 228-282
|
Mutation：T2Q, L5V, A26F, F30V, Y33F, A34F Mutation：T2Q, L5V, A26F, F30V, Y33F, A34F Mutation：T2Q, L5V, A26F, F30V, Y33F, A34F Mutation：T2Q, L5V, A26F, F30V, Y33F, A34F | SO4 SULFATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.6；293 K；PEG 8000, ammonium sulfate, sodium acetate, sodium chloride, TrisHCl, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.50 Å R-free 0.283 |
| 1MVK X-ray structure of the tetrameric mutant of the B1 domain of streptococcal protein G Deposited 2002-09-25 | Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain E
228–282（55 aa）
Fragment：B1 domain, sequence database residues 228-282
Chain F
228–282（55 aa）
Fragment：B1 domain, sequence database residues 228-282
Chain G
228–282（55 aa）
Fragment：B1 domain, sequence database residues 228-282
Chain H
228–282（55 aa）
Fragment：B1 domain, sequence database residues 228-282
|
Mutation：T2Q, L5V, A26F, F30V, Y33F, A34F Mutation：T2Q, L5V, A26F, F30V, Y33F, A34F Mutation：T2Q, L5V, A26F, F30V, Y33F, A34F Mutation：T2Q, L5V, A26F, F30V, Y33F, A34F | SO4 SULFATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.6；293 K；PEG 8000, ammonium sulfate, sodium acetate, sodium chloride, TrisHCl, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.50 Å R-free 0.283 |
| 1MVK X-ray structure of the tetrameric mutant of the B1 domain of streptococcal protein G Deposited 2002-09-25 | Assembly 3 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain I
228–282（55 aa）
Fragment：B1 domain, sequence database residues 228-282
Chain J
228–282（55 aa）
Fragment：B1 domain, sequence database residues 228-282
Chain K
228–282（55 aa）
Fragment：B1 domain, sequence database residues 228-282
Chain L
228–282（55 aa）
Fragment：B1 domain, sequence database residues 228-282
|
Mutation：T2Q, L5V, A26F, F30V, Y33F, A34F Mutation：T2Q, L5V, A26F, F30V, Y33F, A34F Mutation：T2Q, L5V, A26F, F30V, Y33F, A34F Mutation：T2Q, L5V, A26F, F30V, Y33F, A34F | SO4 SULFATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.6；293 K；PEG 8000, ammonium sulfate, sodium acetate, sodium chloride, TrisHCl, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.50 Å R-free 0.283 |
| 1PGA TWO CRYSTAL STRUCTURES OF THE B1 IMMUNOGLOBULIN-BINDING DOMAIN OF STREPTOCOCCAL PROTEIN G AND COMPARISON WITH NMR Deposited 1993-11-23 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
228–282（55 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.07 Å |
| 1PGB TWO CRYSTAL STRUCTURES OF THE B1 IMMUNOGLOBULIN-BINDING DOMAIN OF STREPTOCCOCAL PROTEIN G AND COMPARISON WITH NMR Deposited 1993-11-23 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
228–282（55 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 1.92 Å |
| 1PGX THE 1.66 ANGSTROMS X-RAY STRUCTURE OF THE B2 IMMUNOGLOBULIN-BINDING DOMAIN OF STREPTOCOCCAL PROTEIN G AND COMPARISON TO THE NMR STRUCTURE OF THE B1 DOMAIN Deposited 1992-04-03 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
284–366（83 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 1.66 Å |
| 1PN5 NMR structure of the NALP1 Pyrin domain (PYD) Deposited 2003-06-12 | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
228–282（55 aa）
Fragment：Pyrin domain (PYD)
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 6.5；293 K；Pressure 1
NMR sample composition
1mM NALP1 PYD U-15N,13C; 50mM Na / PO4 - Buffer; 50mM NaCl; 1mM CHAPS; 20mM DTT (D10); 0.02% NaN3; 0.1mM EDTA; protease inhibitor cocktail (Complete, Roche); 95% H2O, 5% D2O | 95% H2O/5% D2O
|
Resolution not provided |
| 1Q10 Ensemble of 40 Structures of the Dimeric Mutant of the B1 Domain of Streptococcal Protein G Deposited 2003-07-18 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
228–282（55 aa）
Fragment：B1 Domain
Chain B
228–282（55 aa）
Fragment：B1 Domain
|
Mutation：T228Q,L231V,F256V,Y259F,A260F Mutation：T228Q,L231V,F256V,Y259F,A260F | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 5.5；298 K；Ionic strength (raw mmCIF value) 50mM sodium phosphate buffer；Pressure ambient
NMR sample composition
1.7mM (in monomer) U-15N,13C, 50mM phosphate buffer, 0.02% NaN3, 92.5% H2O, 7.5% D2O | 92.5% H2O, 7.5% D2O
NMR sample composition
0.85mM (in monomer) U-15N,13C, 0.85mM (in monomer) unlabeled, 50mM phosphate buffer (pH 5.5), 0.02 % NaN3, 92.5% H2O, 7.5% D2O | 92.5% H2O, 7.5% D2O
NMR sample composition
0.85mM (in monomer) U-15N,13C, 0.85mM (in monomer) unlabeled, 50mM phosphate buffer, 0.02% NaN3, 100% D2O | 100% D2O
NMR sample composition
1.7mM (in monomer) unlabeled, 50mM phosphate buffer, 0.02% NaN3, 92.5% H2O, 7.5% D2O | 92.5% H2O, 7.5% D2O
NMR sample composition
1.7mM (in monomer) unlabeled, 50mM phosphate buffer, 0.02% NaN3, 100% D2O | 100% D2O
|
Resolution not provided |
| 2CWB Solution Structure of the Ubiquitin-Associated Domain of Human BMSC-UbP and its Complex with Ubiquitin Deposited 2005-06-17 | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
229–282（54 aa）
Fragment：C-TERMINAL UBA DOMAIN,C-TERMINAL UBA DOMAIN
|
Mutation：I12A | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 6.5；298 K；Ionic strength (raw mmCIF value) 100mM NaCl；Pressure ambient
NMR sample composition
1mM HGB1-UBA U-15N,13C; 20mM phosphate buffer; 90% H2O, 10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 2DEN Solution Structure of the Ubiquitin-Associated Domain of Human BMSC-UbP and its Complex with Ubiquitin Deposited 2006-02-14 | Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
229–282（54 aa）
Fragment：UBA domain,UBA domain
|
Mutation：I12A | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 6.5；298 K；Pressure ambient
NMR sample composition
1mM HGB1-UBA | 20mM phosphate, 100mM NaCl, pH6.5
NMR sample composition
1mM Ubiquitin | 20mM phosphate, 100mM NaCl, pH6.5
|
Resolution not provided |
| 2GB1 A NOVEL, HIGHLY STABLE FOLD OF THE IMMUNOGLOBULIN BINDING DOMAIN OF STREPTOCOCCAL PROTEIN G Deposited 1991-05-15 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
228–282（55 aa）
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR | mmCIF provides none of the parsed conditions | Resolution not provided |
| 2IGD ANISOTROPIC STRUCTURE OF PROTEIN G IGG-BINDING DOMAIN III AT 1.1 ANGSTROM RESOLUTION Deposited 1997-04-30 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
293–352（60 aa）
Fragment：IMMUNOGLOBULIN-BINDING DOMAIN III
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.8；CRYSTALS WERE GROWN BY HANGING DROP VAPOUR DIFFUSION FROM 24-26% PEG 4000, 10MM SODIUM ACETATE AT PH 4.8 AND 0.01% SODIUM AZIDE. CELL PARAMETERS ARE NOT THOSE DETERMINED EXPERIMENTALLY. THEY WERE ADJUSTED ON THE BASIS OF THE ENGH & HUBER DICTIONARY AT THE END OF REFINEMENT. THE EXPERIMENTAL ESTIMATES WERE KNOWN TO HAVE POTENTIAL ERRORS DUE TO INACCURACIES IN THE CRYSTAL-TO-DETECTOR AND WAVELENGTH CALIBRATION., vapor diffusion - hanging drop
|
Resolution 1.10 Å R-free 0.125 |
| 2IGH DETERMINATION OF THE SOLUTION STRUCTURES OF DOMAINS II AND III OF PROTEIN G FROM STREPTOCOCCUS BY 1H NMR Deposited 1992-08-26 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
292–352（61 aa）
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR | mmCIF provides none of the parsed conditions | Resolution not provided |
| 2J52 Solution Structure of GB1 domain Protein G and low and high pressure. Deposited 2006-09-11 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
228–282（55 aa）
Fragment：RESIDUES 228-282
|
Mutation：YES | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 5.4；287 K；Ionic strength (raw mmCIF value) 0.1；Pressure 30.0
NMR sample composition
90%WATER/10%D2O
|
Resolution not provided |
| 2J53 Solution Structure of GB1 domain Protein G and low and high pressure. Deposited 2006-09-11 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
228–282（55 aa）
Fragment：RESIDUES 228-282
|
Mutation：YES | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 5.4；287 K；Ionic strength (raw mmCIF value) 0.1；Pressure 2000.0
NMR sample composition
90%WATER/10%D2O
|
Resolution not provided |
| 2JU6 Solid-State Protein Structure Determination with Proton-Detected Triple Resonance 3D Magic-Angle Spinning NMR Spectroscopy Deposited 2007-08-15 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain X
228–282（55 aa）
|
Mutation：T2Q | No recorded non-water small molecule | SOLID-STATE NMR |
NMR measurement conditions
pH 5.5；281 K；Pressure ambient
NMR sample composition
5 mM [U-13C; U-15N; U-2H] GB1, 50 % isopropyl alcohol, 25 % (4R)-2-Methylpentane-2,4-diol, 50 mM sodium phosphate, Solid Slurry | Solid Slurry
|
Resolution not provided |
| 2K0P Determination of a Protein Structure in the Solid State from NMR Chemical Shifts Deposited 2008-02-11 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
228–282（55 aa）
Fragment：GB1
|
Mutation：T2Q | No recorded non-water small molecule | SOLID-STATE NMR |
NMR measurement conditions
278 K；Pressure 1
NMR sample composition
10 mg/mL [U-100% 13C; U-100% 15N] GB1, 0.5 v/v Methyl Pentane diol, 0.25 v/v Isopropanol | 0.5 v/v Methyl Pentane diol/0.25 v/v Isopropanol
|
Resolution not provided |
| 2KBT Attachment of an NMR-invisible solubility enhancement tag (INSET) using a sortase-mediated protein ligation method Deposited 2008-12-07 | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
228–282（55 aa）
Fragment：SH3 2 domain of Proto-oncogene vav,UNP residues 228-282 of Immunoglobulin G-binding protein G
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 7；298 K；Ionic strength (raw mmCIF value) 150；Pressure ambient
NMR sample composition
20 mM MES-1, 2 mM DTT-2, 150 mM NaCl-3, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 2LGI Atomic Resolution Protein Structures using NMR Chemical Shift Tensors Deposited 2011-07-26 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
229–282（54 aa）
Fragment：2-1 repeat region residues 229-282
|
Not recorded | No recorded non-water small molecule | SOLID-STATE NMR |
NMR measurement conditions
pH 5.5；273 K；Ionic strength (raw mmCIF value) 50；Pressure ambient
NMR sample composition
20 mg U-2-13C-glycerol; U-100% 15N GB1 | solid
|
Resolution not provided |
| 2MBB Solution Structure of the human Polymerase iota UBM1-Ubiquitin Complex Deposited 2013-07-29 | Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
229–282（54 aa）
Fragment：UNP P06654 residues 229-282, UNP Q9UNA4 residues 516-555
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 7；298 K；Ionic strength (raw mmCIF value) 100；Pressure ambient
NMR sample composition
3 mM [U-100% 15N] GB1-UBM1, 3 mM [U-100% 15N] ubiquitin, 100 mM potassium chloride, 25 mM sodium phosphate, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
3 mM [U-100% 13C; U-100% 15N] GB1-UBM1, 3 mM [U-100% 13C; U-100% 15N] ubiquitin, 100 mM potassium chloride, 25 mM sodium phosphate, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
3 mM [U-100% 13C; U-100% 15N] GB1-UBM1, 3 mM [U-100% 13C; U-100% 15N] ubiquitin, 100 mM potassium chloride, 25 mM sodium phosphate, 100% D2O | 100% D2O
NMR sample composition
3 mM [U-100% 13C; U-100% 15N] GB1-UBM1, 3 mM ubiquitin, 100 mM potassium chloride, 25 mM sodium phosphate, 100% D2O | 100% D2O
NMR sample composition
3 mM GB1-UBM1, 3 mM [U-100% 13C; U-100% 15N] ubiquitin, 100 mM potassium chloride, 25 mM sodium phosphate, 100% D2O | 100% D2O
|
Resolution not provided |
| 2N7J Sidechain chi1 distribution in B3 domain of protein G from extensive sets of residual dipolar couplings Deposited 2015-09-12 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
299–352（54 aa）
Fragment：residues 299-352
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 6.5；293 K；Ionic strength (raw mmCIF value) 0.05；Pressure ambient
NMR sample composition
2.5 mM [U-100% 13C; U-100% 15N] wild-type GB3, 95% H2O/5% D2O | 95% H2O/5% D2O
NMR sample composition
1.3 mM [U-13C; U-15N] GB3-K19A/V42E/D47K, 100% D2O | 100% D2O
NMR sample composition
1.3 mM [U-100% 13C; U-100% 15N; U-75% 2H] GB3-K19A/V42E/D47K, 100% D2O | 100% D2O
NMR sample composition
1.3 mM [U-13C; U-15N; U-2H] GB3-K19A/V42E/D47K, 95% H2O/5% D2O | 95% H2O/5% D2O
NMR sample composition
2.0 mM [U-100% 13C; U-100% 15N] GB3-K4A/K19E/V42E, 95% H2O/5% D2O | 95% H2O/5% D2O
NMR sample composition
2.0 mM [U-100% 13C; U-100% 15N] GB3-K4A/K19E/V42E, 100% D2O | 100% D2O
NMR sample composition
2.0 mM [U-100% 13C; U-100% 15N; U-75% 2H] GB3-K4A/K19E/V42E, 100% D2O | 100% D2O
NMR sample composition
2.0 mM [U-13C; U-15N; U-2H] GB3-K4A/K19E/V42E, 95% H2O/5% D2O | 95% H2O/5% D2O
NMR sample composition
2.5 mM [U-100% 13C; U-100% 15N] wild-type GB3, 100% D2O | 100% D2O
NMR sample composition
2.5 mM [U-100% 13C; U-100% 15N; U-75% 2H] GB3-K19A/V42E/D47K, 100% D2O | 100% D2O
NMR sample composition
2.5 mM [U-13C; U-15N; U-2H] wild-type GB3, 95% H2O/5% D2O | 95% H2O/5% D2O
NMR sample composition
0.9 mM [U-13C; U-15N] GB3-K4A/K19E/V42E-CHis6, 95% H2O/5% D2O | 95% H2O/5% D2O
NMR sample composition
0.9 mM [U-13C; U-15N] GB3-K4A/K19E/V42E-CHis6, 100% D2O | 100% D2O
NMR sample composition
0.9 mM [U-100% 13C; U-100% 15N; U-75% 2H] GB3-K4A/K19E/V42E-CHis6, 100% D2O | 100% D2O
NMR sample composition
0.9 mM [U-13C; U-15N; U-2H] GB3-K4A/K19E/V42E-CHis6, 95% H2O/5% D2O | 95% H2O/5% D2O
NMR sample composition
1.3 mM [U-13C; U-15N] GB3-K19A/V42E/D47K, 95% H2O/5% D2O | 95% H2O/5% D2O
|
Resolution not provided |
| 2NMQ Simultaneous determination of protein structure and dynamics using rdcs Deposited 2006-10-23 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
298–352（55 aa）
Fragment：protein GB3(residues 298-352)
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR | mmCIF provides none of the parsed conditions | Resolution not provided |
| 2RMM Solution structure of GB1 A34F mutant Deposited 2007-10-30 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
229–282（54 aa）
Chain B
229–282（54 aa）
|
Mutation：A34F Mutation：A34F | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 5.5；298 K；Ionic strength (raw mmCIF value) 0.05；Pressure ambient
NMR sample composition
2.0mM [U-99% 13C; U-99% 15N] entity, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 2RPV Solution Structure of GB1 with LBT probe Deposited 2008-10-28 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
227–282（56 aa）
Fragment：L2GB
|
Mutation：E38C | LA LANTHANUM (III) ION × 1 | SOLUTION NMR |
NMR measurement conditions
pH 6.5；298 K；Ionic strength (raw mmCIF value) 50；Pressure ambient
NMR sample composition
0.8mM [U-99% 13C; U-99% 15N] L2GB, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 3MP9 Structure of Streptococcal protein G B1 domain at pH 3.0 Deposited 2010-04-26 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
227–282（56 aa）
Fragment：B1 domain (UNP residues 227-282)
|
Not recorded | FMT FORMIC ACID × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 3；290 K；Crystals grown in 3.8M sodium formate, 8% isopropanol, 50mM sodium citrate, pH 5.5. Crystals then soaked in 3.8M sodium formate, 8% isopropanol, 50mM sodium citrate pH 3.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K
|
Resolution 1.20 Å R-free 0.181 |
| 3MP9 Structure of Streptococcal protein G B1 domain at pH 3.0 Deposited 2010-04-26 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
227–282（56 aa）
Fragment：B1 domain (UNP residues 227-282)
|
Not recorded | FMT FORMIC ACID × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 3；290 K；Crystals grown in 3.8M sodium formate, 8% isopropanol, 50mM sodium citrate, pH 5.5. Crystals then soaked in 3.8M sodium formate, 8% isopropanol, 50mM sodium citrate pH 3.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K
|
Resolution 1.20 Å R-free 0.181 |
| 3MP9 Structure of Streptococcal protein G B1 domain at pH 3.0 Deposited 2010-04-26 | Assembly 3 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
227–282（56 aa）
Fragment：B1 domain (UNP residues 227-282)
Chain B
227–282（56 aa）
Fragment：B1 domain (UNP residues 227-282)
|
Not recorded | FMT FORMIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 3；290 K；Crystals grown in 3.8M sodium formate, 8% isopropanol, 50mM sodium citrate, pH 5.5. Crystals then soaked in 3.8M sodium formate, 8% isopropanol, 50mM sodium citrate pH 3.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K
|
Resolution 1.20 Å R-free 0.181 |
| 6CNE Selenomethionine variant (V29SeM) of protein GB1 Deposited 2018-03-08 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
229–282（54 aa）
|
Mutation：L5Sem Non-standard monomer：Yes (specific site not provided by mmCIF) | MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 PO4 PHOSPHATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.9；283.15 K；47% MPD
20% IPA
25 mM sodium acetate pH 4.9
20 mg/ml protein in 25 mM sodium acetate buffer pH 5.5 Non-reducing conditions (no TCEP)
|
Resolution 1.20 Å R-free 0.198 |
| 6CNE Selenomethionine variant (V29SeM) of protein GB1 Deposited 2018-03-08 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
229–282（54 aa）
|
Mutation：L5Sem Non-standard monomer：Yes (specific site not provided by mmCIF) | MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 PO4 PHOSPHATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.9；283.15 K；47% MPD
20% IPA
25 mM sodium acetate pH 4.9
20 mg/ml protein in 25 mM sodium acetate buffer pH 5.5 Non-reducing conditions (no TCEP)
|
Resolution 1.20 Å R-free 0.198 |
| 6L91 X-ray structure of synthetic GB1 domain with the mutation K10(DVA). Deposited 2019-11-07 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
227–282（56 aa）
|
Mutation：K10(DVA) Non-standard monomer：Yes (specific site not provided by mmCIF) | GOL GLYCEROL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；294 K；0.1M NaCacodylate (pH 5.5), 20% PEG 4000
|
Resolution 1.84 Å R-free 0.186 |
| 6L9B X-ray structure of synthetic GB1 domain with mutations K10(DVA), T11A Deposited 2019-11-08 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
227–282（56 aa）
|
Mutation：K10(DVA), T11A Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.6；293 K；0.2M CaCl2, 0.1M sodium acetate (pH 4.6), 30% Isopropanol
|
Resolution 1.95 Å R-free 0.250 |
| 6L9D X-ray structure of synthetic GB1 domain with mutations K10(DVA), T11S Deposited 2019-11-08 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
227–282（56 aa）
|
Mutation：K10DVA, T11S Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；293 K；0.1M Sodium Cacodylate (pH 6), 20% PEG 4000
|
Resolution 1.73 Å R-free 0.243 |
| 6LJI X-ray structure of synthetic GB1 domain with mutations K10(DVA), T11V Deposited 2019-12-16 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
227–282（56 aa）
Fragment：GB1 domain
|
Mutation：K10(DVA), T11V Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；294 K；20% PEG 4000, 0.1M MES pH 6.0
|
Resolution 1.84 Å R-free 0.328 |
| 6LJI X-ray structure of synthetic GB1 domain with mutations K10(DVA), T11V Deposited 2019-12-16 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
227–282（56 aa）
Fragment：GB1 domain
|
Mutation：K10(DVA), T11V Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；294 K；20% PEG 4000, 0.1M MES pH 6.0
|
Resolution 1.84 Å R-free 0.328 |
| 6V9I cryo-EM structure of Cullin5 bound to RING-box protein 2 (Cul5-Rbx2) Deposited 2019-12-13 | Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain C
229–282（54 aa）
|
Not recorded | ZN ZINC ION × 3 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；4 second blot time, blot force 20
|
Resolution 5.20 Å |
| 7QTR GB1 in mammalian cells, 50 uM Deposited 2022-01-15 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
229–282（54 aa）
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 7.4；283 K；Ionic strength (raw mmCIF value) 0；Pressure AMBIENT
NMR sample composition
50 uM [U-13C; U-15N] B1 domain of streptococcal protein G (GB1), 95% H2O/5% D2O | 95% H2O/5% D2O
|
Resolution not provided |
| 7QTS GB1 in mammalian cells, 10 uM Deposited 2022-01-15 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
229–282（54 aa）
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 7.4；283 K；Ionic strength (raw mmCIF value) 0；Pressure AMBIENT
NMR sample composition
10 uM [U-13C; U-15N] GB1, in-cell | in-cell
|
Resolution not provided |
| 7RXC CryoEM structure of KDELR with Legobody Deposited 2021-08-22 | Assembly 1 Insufficient information Heteromer；Protein × 5 PDB declaration: pentameric（5） Consistent with protein count |
Chain B
295–352（58 aa）
|
Not recorded | POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 4 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 7RXD CryoEM structure of RBD domain of COVID-19 in complex with Legobody Deposited 2021-08-22 | Assembly 1 Insufficient information Heteromer；Protein × 5 PDB declaration: pentameric（5） Consistent with protein count |
Chain B
295–352（58 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 8JXR Structure of nanobody-bound DRD1_LSD complex Deposited 2023-07-01 | Assembly 1 Insufficient information Heteromer；Protein × 5 PDB declaration: pentameric（5） Consistent with protein count |
Chain C
295–352（58 aa）
|
Mutation：E360Q,K363A,D364F,T367I,R368L | 7LD (8alpha)-N,N-diethyl-6-methyl-9,10-didehydroergoline-8-carboxamide × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.57 Å |
| 8JXS Structure of nanobody-bound DRD1_PF-6142 complex Deposited 2023-07-01 | Assembly 1 Insufficient information Heteromer；Protein × 5 PDB declaration: pentameric（5） Consistent with protein count |
Chain C
295–352（58 aa）
|
Mutation：E360Q,K363A,D364F,T367I,R368L,D404E,A405H | V6X 4-[3-methyl-4-(6-methylimidazo[1,2-a]pyrazin-5-yl)phenoxy]furo[3,2-c]pyridine × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 8T0G Backbone Dialkylation in Peptide Hairpins: Natural Backbone Prototype Deposited 2023-06-01 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
267–282（16 aa）
|
Mutation：T13A Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 6.3；298 K；Ionic strength (raw mmCIF value) 250；Pressure 1
NMR sample composition
2 mM GB1 C-terminal Hairpin Mutant: Ala13 variant, 50 mM sodium phosphate, 0.2 mM DSS, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 9BDT Apolipoprotein B 100 bound to LDL receptor and legobody Deposited 2024-04-12 | Assembly 1 Insufficient information Heteromer；Protein × 7 PDB declaration: heptameric（7） Consistent with protein count |
Chain B
295–352（58 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 4 CA CALCIUM ION × 7 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.40 Å |
| 9CHT Human E3 ligase E6AP in complex with HPV16-E6 and p53 Deposited 2024-07-02 | Assembly 1 Insufficient information Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain B
228–282（55 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.54 Å |
| 9COO Nanobody 4 bound to Apolipoprotein B 100 Deposited 2024-07-17 | Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain B
295–352（58 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 CA CALCIUM ION × 3 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.73 Å |
| 9G4T Beta carbonic anhydrase CsoSCA from the Halothiobacillus neapolitanus alpha-carboxysome Deposited 2024-07-16 | Assembly 1 Insufficient information Homooligomer；Protein × 6 PDB declaration: monomeric（1） Review required |
Chain A
229–282（54 aa）
|
Not recorded | ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.51 Å |
| 9JA5 Cryo-EM structure of Tdk1-Bdf1 complex Deposited 2024-08-24 | Assembly 1 Insufficient information Homooligomer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain A
228–282（55 aa）
Chain B
228–282（55 aa）
Chain C
228–282（55 aa）
Chain D
228–282（55 aa）
Chain E
228–282（55 aa）
Chain F
228–282（55 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；blot for 5 seconds before plunging
|
Resolution 2.70 Å |
| 9JA6 Cryo-EM structure of Tdk1 tetramer complex Deposited 2024-08-24 | Assembly 1 Insufficient information Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
228–282（55 aa）
Chain B
228–282（55 aa）
Chain C
228–282（55 aa）
Chain D
228–282（55 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；blot for 5 seconds before plunging
|
Resolution 4.40 Å |
| 9W3K GPR151-Legobody complex Deposited 2025-07-29 | Assembly 1 Insufficient information Heteromer；Protein × 5 PDB declaration: pentameric（5） Consistent with protein count |
Chain C
295–352（58 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.08 Å |