Current Protein Identity:P07291
New Search
Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1B7T MYOSIN DIGESTED BY PAPAIN Deposited 1999-01-15 | Assembly 1 Protein heterocomplex Heteromer;Protein × 3 PDB declaration: trimeric(3) Consistent with protein count |
Chain Z
2–157(156 aa)
Fragment:PAPAIN DIGESTED, SUBFRAGMENT 1 (S1)
|
Not recorded | MG MAGNESIUM ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 8;pH 8.0
|
Resolution 2.50 Å R-free 0.297 |
| 1DFK NUCLEOTIDE-FREE SCALLOP MYOSIN S1-NEAR RIGOR STATE Deposited 1999-11-19 | Assembly 1 Protein heterocomplex Heteromer;Protein × 3 PDB declaration: trimeric(3) Consistent with protein count |
Chain Z
4–155(152 aa)
Fragment:ESSENTIAL LIGHT CHAIN
|
Not recorded | CA CALCIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 7;277 K;PEG8000, ammonium sulfate, cacodylate, glycerol , pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 4K
|
Resolution 4.20 Å R-free 0.410 |
| 1DFL SCALLOP MYOSIN S1 COMPLEXED WITH MGADP:VANADATE-TRANSITION STATE Deposited 1999-11-19 | Assembly 1 Protein heterocomplex Heteromer;Protein × 3 PDB declaration: trimeric(3) Consistent with protein count |
Chain Z
4–155(152 aa)
Fragment:ESSENTIAL LIGHT CHAIN
|
Not recorded | MG MAGNESIUM ION × 2 VO4 VANADATE ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 6;277 K;PEG 8000, MES, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 4K
|
Resolution 4.20 Å R-free 0.400 |
| 1DFL SCALLOP MYOSIN S1 COMPLEXED WITH MGADP:VANADATE-TRANSITION STATE Deposited 1999-11-19 | Assembly 2 Protein heterocomplex Heteromer;Protein × 3 PDB declaration: trimeric(3) Consistent with protein count |
Chain X
4–155(152 aa)
Fragment:ESSENTIAL LIGHT CHAIN
|
Not recorded | MG MAGNESIUM ION × 2 VO4 VANADATE ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 6;277 K;PEG 8000, MES, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 4K
|
Resolution 4.20 Å R-free 0.400 |
| 1KK7 SCALLOP MYOSIN IN THE NEAR RIGOR CONFORMATION Deposited 2001-12-06 | Assembly 1 Protein heterocomplex Heteromer;Protein × 3 PDB declaration: trimeric(3) Consistent with protein count |
Chain Z
1–156(156 aa)
Fragment:ESSENTIAL LIGHT CHAIN
|
Not recorded | SO4 SULFATE ION × 1 MG MAGNESIUM ION × 2 CA CALCIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP;pH 6.3;277 K;PEG 5000, ammonium sulphate, MgCl2, gycerol, cacodylate, pH 6.3, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
Resolution 3.20 Å R-free 0.313 |
| 1KK8 SCALLOP MYOSIN (S1-ADP-BeFx) IN THE ACTIN-DETACHED CONFORMATION Deposited 2001-12-06 | Assembly 1 Protein heterocomplex Heteromer;Protein × 3 PDB declaration: trimeric(3) Consistent with protein count |
Chain C
1–154(154 aa)
Fragment:MYOSIN ESSENTIAL LIGHT CHAIN
|
Not recorded | MG MAGNESIUM ION × 2 BEF BERYLLIUM TRIFLUORIDE ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 GOL GLYCEROL × 1 CA CALCIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP;pH 8.5;277 K;PEG 20000, magnesium chloride, ethylene glycol, Tris HCl, ADP-berillium floride, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
Resolution 2.30 Å R-free 0.269 |
| 1KQM SCALLOP MYOSIN S1-AMPPNP IN THE ACTIN-DETACHED CONFORMATION Deposited 2002-01-07 | Assembly 1 Protein heterocomplex Heteromer;Protein × 3 PDB declaration: trimeric(3) Consistent with protein count |
Chain C
1–156(156 aa)
|
Not recorded | MG MAGNESIUM ION × 2 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 CA CALCIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP;pH 8.5;277 K;PEG 20000, magnesium chloride, ethylene glycol, Tris HCl, AMPPNP, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
Resolution 3.00 Å R-free 0.316 |
| 1KWO SCALLOP MYOSIN S1-ATPgammaS-p-PDM IN THE ACTIN-DETACHED CONFORMATION Deposited 2002-01-30 | Assembly 1 Protein heterocomplex Heteromer;Protein × 3 PDB declaration: trimeric(3) Consistent with protein count |
Chain C
1–156(156 aa)
|
Not recorded | MG MAGNESIUM ION × 2 AGS PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER × 1 PDM 4-[4-(2,5-DIOXO-PYRROLIDIN-1-YL)-PHENYLAMINO]-4-HYDROXY-BUTYRIC ACID × 1 CA CALCIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP;pH 8.5;277 K;PEG 20000, magnesium chloride, ethylene glycol, Tris HCl, ATPgammaS, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
Resolution 3.80 Å R-free 0.328 |
| 1L2O SCALLOP MYOSIN S1-ADP-p-PDM IN THE ACTIN-DETACHED CONFORMATION Deposited 2002-02-22 | Assembly 1 Protein heterocomplex Heteromer;Protein × 3 PDB declaration: trimeric(3) Consistent with protein count |
Chain C
1–156(156 aa)
|
Not recorded | MG MAGNESIUM ION × 2 PDM 4-[4-(2,5-DIOXO-PYRROLIDIN-1-YL)-PHENYLAMINO]-4-HYDROXY-BUTYRIC ACID × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP;pH 8.5;277 K;PEG 20000, magnesium chloride, ethylene glycol, Tris HCl, ATPgammaS, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
Resolution 2.80 Å R-free 0.327 |
| 1QVI Crystal structure of scallop myosin S1 in the pre-power stroke state to 2.6 Angstrom resolution: flexibility and function in the head Deposited 2003-08-27 | Assembly 1 Protein heterocomplex Heteromer;Protein × 3 PDB declaration: trimeric(3) Consistent with protein count |
Chain Z
1–156(156 aa)
|
Not recorded | MG MAGNESIUM ION × 2 VO4 VANADATE ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP;pH 6;298 K;MES, MgCl2, ADP, Na3VO4, polyethylene glycol 8K, glycerol, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 2.54 Å R-free 0.266 |
| 1S5G Structure of Scallop myosin S1 reveals a novel nucleotide conformation Deposited 2004-01-20 | Assembly 1 Protein heterocomplex Heteromer;Protein × 3 PDB declaration: trimeric(3) Consistent with protein count |
Chain Z
1–156(156 aa)
|
Not recorded | SO4 SULFATE ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 CA CALCIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP;pH 6.5;300 K;PEG 8000, magnesium chloride, ADP, Glycerol, MES, TMAO, cacodylate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 300K
|
Resolution 3.10 Å R-free 0.269 |
| 1SCM STRUCTURE OF THE REGULATORY DOMAIN OF SCALLOP MYOSIN AT 2.8 ANGSTROMS RESOLUTION Deposited 1994-01-06 | Assembly 1 Protein heterocomplex Heteromer;Protein × 3 PDB declaration: trimeric(3) Consistent with protein count |
Chain C
4–152(149 aa)
|
Not recorded | CA CALCIUM ION × 2 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.80 Å |
| 1SR6 Structure of nucleotide-free scallop myosin S1 Deposited 2004-03-22 | Assembly 1 Protein heterocomplex Heteromer;Protein × 3 PDB declaration: trimeric(3) Consistent with protein count |
Chain C
1–156(156 aa)
|
Not recorded | SO4 SULFATE ION × 1 MG MAGNESIUM ION × 1 CA CALCIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 7;300 K;6% PEG 8000, 50mM ammonium sulfate, 8% glycerol, 10mM magnesium chloride, 50mM sodium cacodylate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 300K
|
Resolution 2.75 Å R-free 0.286 |
| 1WDC SCALLOP MYOSIN REGULATORY DOMAIN Deposited 1996-01-19 | Assembly 1 Protein heterocomplex Heteromer;Protein × 3 PDB declaration: trimeric(3) Consistent with protein count |
Chain C
1–156(156 aa)
Fragment:PROTEOLYTIC FRAGMENT, REGULATORY DOMAIN
|
Not recorded | MG MAGNESIUM ION × 1 CA CALCIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7;pH 7.0
|
Resolution 2.00 Å R-free 0.281 |
| 2W4T ISOMETRICALLY CONTRACTING INSECT ASYNCHRONOUS FLIGHT MUSCLE Deposited 2008-12-02 | Assembly 3 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain Z
5–155(151 aa)
Fragment:RESIDUES 5-155
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
20 MM MOPS BUFFER, 5 MM NAN3, AND MGCL2, ATP, CACL2, AND EGTA IN VARYING MILLIMOLAR MILLIMOLAR CONCENTRATIONS;20 MM MOPS BUFFER, 5 MM NAN3, AND MGCL2, ATP, CACL2, AND EGTA IN VARYING MILLIMOLAR MILLIMOLAR CONCENTRATIONS
cryo-EM vitrification conditions
Cryogen HELIUM
|
Resolution 35.00 Å |
| 2W4V Isometrically contracting insect asynchronous flight muscle quick frozen after a quick release step Deposited 2008-12-02 | Assembly 1 Protein heterocomplex Heteromer;Protein × 3 PDB declaration: trimeric(3) Consistent with protein count |
Chain Z
5–155(151 aa)
Fragment:RESIDUES 5-155
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
20 MM MOPS BUFFER, 5 MM NAN3, AND MGCL2, ATP, CACL2, AND EGTA IN VARYING MILLIMOLAR CONCENTRATIONS;20 MM MOPS BUFFER, 5 MM NAN3, AND MGCL2, ATP, CACL2, AND EGTA IN VARYING MILLIMOLAR CONCENTRATIONS
cryo-EM vitrification conditions
Cryogen HELIUM;SMASH AGAINST A LIQUID HELIUM COOLED GOLD COATED COPPER MIRROR
|
Resolution 35.00 Å |
| 2W4W Isometrically contracting insect asynchronous flight muscle quick frozen after a quick stretch step Deposited 2008-12-02 | Assembly 1 Protein heterocomplex Heteromer;Protein × 3 PDB declaration: trimeric(3) Consistent with protein count |
Chain Z
5–155(151 aa)
Fragment:RESIDUES 5-155
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
20 MM MOPS BUFFER, 5 MM NAN3, AND MGCL2, ATP, CACL2, AND EGTA IN VARYING MILLIMOLAR CONCENTRATIONS;20 MM MOPS BUFFER, 5 MM NAN3, AND MGCL2, ATP, CACL2, AND EGTA IN VARYING MILLIMOLAR CONCENTRATIONS
cryo-EM vitrification conditions
Cryogen HELIUM;SMASH AGAINST A LIQUID HELIUM COOLED GOLD COATED COPPER MIRROR
|
Resolution 35.00 Å |
| 3JTD Calcium-free Scallop Myosin Regulatory Domain with ELC-D19A Point Mutation Deposited 2009-09-11 | Assembly 1 Protein heterocomplex Heteromer;Protein × 3 PDB declaration: trimeric(3) Consistent with protein count |
Chain C
2–157(156 aa)
|
Mutation:D19A | MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 8.3;277 K;100 mM bicine pH 8.3, 45 mM ammonium sulfate, 5 mM magnesium chloride, 2.5 mM EGTA, 3.0 mM sodium azide, 15% (wt/vol) PEG 4000, 4% (vol/vol) PEG 200, 6% (wt/vol) 2,3-butanediol, Hanging drop vapor diffusion, temperature 277K, VAPOR DIFFUSION, HANGING DROP
|
Resolution 2.57 Å R-free 0.248 |
| 3JVT Calcium-bound Scallop Myosin Regulatory Domain (Lever Arm) with Reconstituted Complete Light Chains Deposited 2009-09-17 | Assembly 1 Protein heterocomplex Heteromer;Protein × 3 PDB declaration: trimeric(3) Consistent with protein count |
Chain C
2–157(156 aa)
|
Not recorded | MG MAGNESIUM ION × 1 CA CALCIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 8.3;277 K;150 mM HEPES pH 7.0, 50 mM Ammonium Sulfate, 0.5 mM Calcium Chloride, 5 mM Magnesium Chloride, 5 mM Sodium Azide, 16.5% (wt/vol) PEG 4000 combined with equal volume of 16.9 mg/ml protein in 5 mM HEPES pH 7, 20 mM NaCl, 2 mM Magnesium Chloride, 0.2 mM Calcium Chloride, 0.1 mM EGTA, 3.0 mM Sodium Azide, Hanging drop vapor diffusion, temperature 277K, VAPOR DIFFUSION, HANGING DROP
|
Resolution 2.10 Å R-free 0.231 |