Current Protein Identity：P11540
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1A19 BARSTAR (FREE), C82A MUTANT Deposited 1997-12-25 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–89（89 aa）
Chain B
1–89（89 aa）
|
Mutation：C82A Mutation：C82A | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.5；C82A MUTANT WAS CRYSTALLIZED FROM 40-60% AMMONIUM SULFATE, 50MM PHOSPHATE, PH=6.5. PROTEIN CONC=45 MG/ML. C40A AND DTNB LABELLED CRYSTALS ALSO CRYSTALLIZED BUT DID NOT DIFFRACT TO HIGH RESOLUTION.
|
Resolution 2.76 Å R-free 0.292 |
| 1AB7 NMR 15N RELAXATION AND STRUCTURAL STUDIES REVEAL CONFORMATIONAL EXCHANGE IN BARSTAR C40/82A, 30 STRUCTURES Deposited 1997-02-04 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1–89（89 aa）
|
Mutation：C40A, C82A | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 6.7；303 K
|
Resolution not provided |
| 1AY7 RIBONUCLEASE SA COMPLEX WITH BARSTAR Deposited 1997-11-14 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
1–89（89 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7；pH 7.0
|
Resolution 1.70 Å R-free 0.190 |
| 1B27 STRUCTURAL RESPONSE TO MUTATION AT A PROTEIN-PROTEIN INTERFACE Deposited 1998-12-04 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain D
1–89（89 aa）
|
Mutation：C40A, C82A | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 8；18-24% PEG-8K 0.2 M AMMONIUM SULPHATE 0.1 M TRIS PH8.0
|
Resolution 2.10 Å R-free 0.259 |
| 1B27 STRUCTURAL RESPONSE TO MUTATION AT A PROTEIN-PROTEIN INTERFACE Deposited 1998-12-04 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain E
1–89（89 aa）
|
Mutation：C40A, C82A | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 8；18-24% PEG-8K 0.2 M AMMONIUM SULPHATE 0.1 M TRIS PH8.0
|
Resolution 2.10 Å R-free 0.259 |
| 1B27 STRUCTURAL RESPONSE TO MUTATION AT A PROTEIN-PROTEIN INTERFACE Deposited 1998-12-04 | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain F
1–89（89 aa）
|
Mutation：C40A, C82A | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 8；18-24% PEG-8K 0.2 M AMMONIUM SULPHATE 0.1 M TRIS PH8.0
|
Resolution 2.10 Å R-free 0.259 |
| 1B2S STRUCTURAL RESPONSE TO MUTATION AT A PROTEIN-PROTEIN INTERFACE Deposited 1998-11-30 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain D
1–89（89 aa）
|
Mutation：T43A | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 8；18% PEG-4K 0.1M TRIS PH8.0 0.2M LI2SO4
|
Resolution 1.82 Å R-free 0.249 |
| 1B2S STRUCTURAL RESPONSE TO MUTATION AT A PROTEIN-PROTEIN INTERFACE Deposited 1998-11-30 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain E
1–89（89 aa）
|
Mutation：T43A | SO4 SULFATE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 8；18% PEG-4K 0.1M TRIS PH8.0 0.2M LI2SO4
|
Resolution 1.82 Å R-free 0.249 |
| 1B2S STRUCTURAL RESPONSE TO MUTATION AT A PROTEIN-PROTEIN INTERFACE Deposited 1998-11-30 | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain F
1–89（89 aa）
|
Mutation：T43A | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 8；18% PEG-4K 0.1M TRIS PH8.0 0.2M LI2SO4
|
Resolution 1.82 Å R-free 0.249 |
| 1B2U STRUCTURAL RESPONSE TO MUTATION AT A PROTEIN-PROTEIN INTERFACE Deposited 1998-12-01 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain D
1–89（89 aa）
|
Mutation：D36A | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.5；21% PEG-8K 0.2 M AMMONIUM SULPHATE 0.1 M NA CACODYLATE PH6.5
|
Resolution 2.10 Å R-free 0.276 |
| 1B2U STRUCTURAL RESPONSE TO MUTATION AT A PROTEIN-PROTEIN INTERFACE Deposited 1998-12-01 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain E
1–89（89 aa）
|
Mutation：D36A | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.5；21% PEG-8K 0.2 M AMMONIUM SULPHATE 0.1 M NA CACODYLATE PH6.5
|
Resolution 2.10 Å R-free 0.276 |
| 1B2U STRUCTURAL RESPONSE TO MUTATION AT A PROTEIN-PROTEIN INTERFACE Deposited 1998-12-01 | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain F
1–89（89 aa）
|
Mutation：D36A | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.5；21% PEG-8K 0.2 M AMMONIUM SULPHATE 0.1 M NA CACODYLATE PH6.5
|
Resolution 2.10 Å R-free 0.276 |
| 1B3S STRUCTURAL RESPONSE TO MUTATION AT A PROTEIN-PROTEIN INTERFACE Deposited 1998-12-01 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain D
1–89（89 aa）
|
Mutation：Y30F | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 8；0.2M (NH4)2SO4; 0.1M TRIS/HCL PH 8.0: 22% PEG-8000
|
Resolution 2.39 Å R-free 0.324 |
| 1B3S STRUCTURAL RESPONSE TO MUTATION AT A PROTEIN-PROTEIN INTERFACE Deposited 1998-12-01 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain E
1–89（89 aa）
|
Mutation：Y30F | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 8；0.2M (NH4)2SO4; 0.1M TRIS/HCL PH 8.0: 22% PEG-8000
|
Resolution 2.39 Å R-free 0.324 |
| 1B3S STRUCTURAL RESPONSE TO MUTATION AT A PROTEIN-PROTEIN INTERFACE Deposited 1998-12-01 | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain F
1–89（89 aa）
|
Mutation：Y30F | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 8；0.2M (NH4)2SO4; 0.1M TRIS/HCL PH 8.0: 22% PEG-8000
|
Resolution 2.39 Å R-free 0.324 |
| 1BGS RECOGNITION BETWEEN A BACTERIAL RIBONUCLEASE, BARNASE, AND ITS NATURAL INHIBITOR, BARSTAR Deposited 1993-11-02 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain E
1–89（89 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.60 Å |
| 1BGS RECOGNITION BETWEEN A BACTERIAL RIBONUCLEASE, BARNASE, AND ITS NATURAL INHIBITOR, BARSTAR Deposited 1993-11-02 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain F
1–89（89 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.60 Å |
| 1BGS RECOGNITION BETWEEN A BACTERIAL RIBONUCLEASE, BARNASE, AND ITS NATURAL INHIBITOR, BARSTAR Deposited 1993-11-02 | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain G
1–89（89 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.60 Å |
| 1BRS PROTEIN-PROTEIN RECOGNITION: CRYSTAL STRUCTURAL ANALYSIS OF A BARNASE-BARSTAR COMPLEX AT 2.0-A RESOLUTION Deposited 1994-03-11 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain D
1–89（89 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.00 Å |
| 1BRS PROTEIN-PROTEIN RECOGNITION: CRYSTAL STRUCTURAL ANALYSIS OF A BARNASE-BARSTAR COMPLEX AT 2.0-A RESOLUTION Deposited 1994-03-11 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain E
1–89（89 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.00 Å |
| 1BRS PROTEIN-PROTEIN RECOGNITION: CRYSTAL STRUCTURAL ANALYSIS OF A BARNASE-BARSTAR COMPLEX AT 2.0-A RESOLUTION Deposited 1994-03-11 | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain F
1–89（89 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.00 Å |
| 1BTA THREE-DIMENSIONAL SOLUTION STRUCTURE AND 13C ASSIGNMENTS OF BARSTAR USING NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY Deposited 1994-05-09 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1–89（89 aa）
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR | mmCIF provides none of the parsed conditions | Resolution not provided |
| 1BTB THREE-DIMENSIONAL SOLUTION STRUCTURE AND 13C ASSIGNMENTS OF BARSTAR USING NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY Deposited 1994-05-09 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1–89（89 aa）
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR | mmCIF provides none of the parsed conditions | Resolution not provided |
| 1L1K NMR Identification and Characterization of the Flexible Regions in the 160 KD Molten Globule-like Aggregate of Barstar at Low pH Deposited 2002-02-18 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1–20（20 aa）
Fragment：Flexible region (residues 1-20)
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 2.7；298 K；Pressure 1
NMR sample composition
1.2 mM Barstar U-15N, 13C; | 90 % H2O, 10& D2O; pH 2.7
|
Resolution not provided |
| 1X1U Water-mediate interaction at aprotein-protein interface Deposited 2005-04-13 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain D
1–89（89 aa）
|
Mutation：C40A, C82A | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；VAPOR DIFFUSION, HANGING DROP
|
Resolution 2.30 Å R-free 0.270 |
| 1X1U Water-mediate interaction at aprotein-protein interface Deposited 2005-04-13 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain E
1–89（89 aa）
|
Mutation：C40A, C82A | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；VAPOR DIFFUSION, HANGING DROP
|
Resolution 2.30 Å R-free 0.270 |
| 1X1U Water-mediate interaction at aprotein-protein interface Deposited 2005-04-13 | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain F
1–89（89 aa）
|
Mutation：C40A, C82A | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；VAPOR DIFFUSION, HANGING DROP
|
Resolution 2.30 Å R-free 0.270 |
| 1X1W Water-mediate interaction at aprotein-protein interface Deposited 2005-04-14 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain D
0–89（90 aa）
|
Mutation：C40A, C82A, E80A | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；VAPOR DIFFUSION, HANGING DROP
|
Resolution 2.10 Å R-free 0.265 |
| 1X1W Water-mediate interaction at aprotein-protein interface Deposited 2005-04-14 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain E
0–89（90 aa）
|
Mutation：C40A, C82A, E80A | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；VAPOR DIFFUSION, HANGING DROP
|
Resolution 2.10 Å R-free 0.265 |
| 1X1W Water-mediate interaction at aprotein-protein interface Deposited 2005-04-14 | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain F
0–89（90 aa）
|
Mutation：C40A, C82A, E80A | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；VAPOR DIFFUSION, HANGING DROP
|
Resolution 2.10 Å R-free 0.265 |
| 1X1X Water-mediate interaction at aprotein-protein interface Deposited 2005-04-15 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain D
1–89（89 aa）
|
Mutation：C40A, E76A, C82A | No recorded non-water small molecule | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.30 Å R-free 0.237 |
| 1X1X Water-mediate interaction at aprotein-protein interface Deposited 2005-04-15 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain E
1–89（89 aa）
|
Mutation：C40A, E76A, C82A | No recorded non-water small molecule | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.30 Å R-free 0.237 |
| 1X1X Water-mediate interaction at aprotein-protein interface Deposited 2005-04-15 | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain F
1–89（89 aa）
|
Mutation：C40A, E76A, C82A | No recorded non-water small molecule | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.30 Å R-free 0.237 |
| 1X1Y Water-mediate interaction at aprotein-protein interface Deposited 2005-04-15 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain D
0–89（90 aa）
|
Mutation：D35A, C40A, C82A | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；VAPOR DIFFUSION, HANGING DROP
|
Resolution 1.90 Å R-free 0.250 |
| 1X1Y Water-mediate interaction at aprotein-protein interface Deposited 2005-04-15 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain E
0–89（90 aa）
|
Mutation：D35A, C40A, C82A | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；VAPOR DIFFUSION, HANGING DROP
|
Resolution 1.90 Å R-free 0.250 |
| 1X1Y Water-mediate interaction at aprotein-protein interface Deposited 2005-04-15 | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain F
0–89（90 aa）
|
Mutation：D35A, C40A, C82A | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；VAPOR DIFFUSION, HANGING DROP
|
Resolution 1.90 Å R-free 0.250 |
| 2HXX Aminotryptophan Barstar Deposited 2006-08-04 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
2–90（89 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；277 K；2.4 M Ammonium sulfate, 50 mM Tris/Hcl, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
Resolution 2.00 Å R-free 0.290 |
| 2HXX Aminotryptophan Barstar Deposited 2006-08-04 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
2–90（89 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；277 K；2.4 M Ammonium sulfate, 50 mM Tris/Hcl, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
Resolution 2.00 Å R-free 0.290 |
| 2ZA4 Crystal Structural Analysis of Barnase-barstar Complex Deposited 2007-10-01 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
1–90（90 aa）
|
Mutation：D39A, C40A, C82A | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；20% PEG 6000, 0.1M HEPES (pH 7.0), 1.0M lithium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.58 Å R-free 0.200 |
| 2ZA4 Crystal Structural Analysis of Barnase-barstar Complex Deposited 2007-10-01 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain D
1–90（90 aa）
|
Mutation：D39A, C40A, C82A | CL CHLORIDE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；20% PEG 6000, 0.1M HEPES (pH 7.0), 1.0M lithium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.58 Å R-free 0.200 |
| 2ZA4 Crystal Structural Analysis of Barnase-barstar Complex Deposited 2007-10-01 | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
1–90（90 aa）
|
Mutation：D39A, C40A, C82A | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；20% PEG 6000, 0.1M HEPES (pH 7.0), 1.0M lithium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.58 Å R-free 0.200 |
| 2ZA4 Crystal Structural Analysis of Barnase-barstar Complex Deposited 2007-10-01 | Assembly 6 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain D
1–90（90 aa）
|
Mutation：D39A, C40A, C82A | CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；20% PEG 6000, 0.1M HEPES (pH 7.0), 1.0M lithium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.58 Å R-free 0.200 |
| 3DA7 A conformationally strained, circular permutant of barnase Deposited 2008-05-28 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain D
1–90（90 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；277 K；1M Tris, 14% peg 8k, 150mM (NH4)2SO4, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.25 Å R-free 0.250 |
| 3DA7 A conformationally strained, circular permutant of barnase Deposited 2008-05-28 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain C
1–90（90 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；277 K；1M Tris, 14% peg 8k, 150mM (NH4)2SO4, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.25 Å R-free 0.250 |
| 3DA7 A conformationally strained, circular permutant of barnase Deposited 2008-05-28 | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain F
1–90（90 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；277 K；1M Tris, 14% peg 8k, 150mM (NH4)2SO4, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.25 Å R-free 0.250 |
| 3DA7 A conformationally strained, circular permutant of barnase Deposited 2008-05-28 | Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain H
1–90（90 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；277 K；1M Tris, 14% peg 8k, 150mM (NH4)2SO4, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.25 Å R-free 0.250 |
| 6PQK Cryogenic crystal structure of barnase A43C/S80C bound to barstar C40A/S59C/A67C/C82A Deposited 2019-07-09 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain D
1–90（90 aa）
|
Mutation：C40A, S59C, A67C, C82A | PO4 PHOSPHATE ION × 3 EDO 1,2-ETHANEDIOL × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；50mM NaPO4 pH 6.5, 25% PEG 8K, 0.1M AmSO4
4uL protein at 10-15 mg/mL in H2O + 4uL motherliquor per hanging drop, over 1 mL motherliquor in well.
cryoprotectant used before freezing was: 12.5% glycerol + 12.5% ethylene glycol
|
Resolution 1.20 Å R-free 0.160 |
| 6PQK Cryogenic crystal structure of barnase A43C/S80C bound to barstar C40A/S59C/A67C/C82A Deposited 2019-07-09 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
1–90（90 aa）
|
Mutation：C40A, S59C, A67C, C82A | PO4 PHOSPHATE ION × 1 EDO 1,2-ETHANEDIOL × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；50mM NaPO4 pH 6.5, 25% PEG 8K, 0.1M AmSO4
4uL protein at 10-15 mg/mL in H2O + 4uL motherliquor per hanging drop, over 1 mL motherliquor in well.
cryoprotectant used before freezing was: 12.5% glycerol + 12.5% ethylene glycol
|
Resolution 1.20 Å R-free 0.160 |
| 7MRX Cryogenic crystal structure of barnase A43C/S80C bound to barstar C40A/C82A Deposited 2021-05-10 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
1–90（90 aa）
|
Mutation：C41A, C83A | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；50mM NaPO4, 25% PEG 8K, 0.1M AmSO4 4uL protein at 10-15 mg/mL in H2O + 4uL motherliquor per hanging drop, over 1 mL motherliquor in well. cryoprotectant used before freezing was: 25% MPD
|
Resolution 2.29 Å R-free 0.229 |
| 7MRX Cryogenic crystal structure of barnase A43C/S80C bound to barstar C40A/C82A Deposited 2021-05-10 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain D
1–90（90 aa）
|
Mutation：C41A, C83A | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；50mM NaPO4, 25% PEG 8K, 0.1M AmSO4 4uL protein at 10-15 mg/mL in H2O + 4uL motherliquor per hanging drop, over 1 mL motherliquor in well. cryoprotectant used before freezing was: 25% MPD
|
Resolution 2.29 Å R-free 0.229 |
| 7MRX Cryogenic crystal structure of barnase A43C/S80C bound to barstar C40A/C82A Deposited 2021-05-10 | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain F
1–90（90 aa）
|
Mutation：C41A, C83A | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；50mM NaPO4, 25% PEG 8K, 0.1M AmSO4 4uL protein at 10-15 mg/mL in H2O + 4uL motherliquor per hanging drop, over 1 mL motherliquor in well. cryoprotectant used before freezing was: 25% MPD
|
Resolution 2.29 Å R-free 0.229 |