Current Protein Identity：P30665
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 28VY sCMGE assembled on ARS1 DNA with Sld2 and RPA Deposited 2026-02-23 | Assembly 1 Protein–DNA Heteromer；Protein × 13 PDB declaration: 15-meric（15） Consistent with all polymers |
Chain 4
1–933（933 aa）
|
Not recorded | MG MAGNESIUM ION × 6 ZN ZINC ION × 7 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 5 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.70 Å |
| 3JA8 Cryo-EM structure of the MCM2-7 double hexamer Deposited 2015-05-09 | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain 4
1–933（933 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 3JC5 Structure of the eukaryotic replicative CMG helicase and pumpjack motion Deposited 2015-11-24 | Assembly 1 Protein heterocomplex Heteromer；Protein × 11 PDB declaration: undecameric（11） Consistent with protein count |
Chain 4
1–933（933 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
20 mM Tris acetate, pH 7.5, 40 mM potassium glutamate, 2 mM DTT, 0.1 mM EDTA；pH 7.5；20 mM Tris acetate, pH 7.5, 40 mM potassium glutamate, 2 mM DTT, 0.1 mM EDTA
cryo-EM vitrification conditions
Blot for 3 seconds before plunging；Cryogen ETHANE；Blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK IV).
|
Resolution 4.70 Å |
| 3JC6 Structure of the eukaryotic replicative CMG helicase and pumpjack motion Deposited 2015-11-24 | Assembly 1 Protein heterocomplex Heteromer；Protein × 11 PDB declaration: undecameric（11） Consistent with protein count |
Chain 4
1–933（933 aa）
|
Not recorded | ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
20 mM Tris acetate, pH 7.5, 40 mM potassium glutamate, 2 mM DTT, 0.1 mM EDTA；pH 7.5；20 mM Tris acetate, pH 7.5, 40 mM potassium glutamate, 2 mM DTT, 0.1 mM EDTA
cryo-EM vitrification conditions
Blot for 3 seconds before plunging；Cryogen ETHANE；Blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK IV).
|
Resolution 3.70 Å R-free 0.331 |
| 3JC7 Structure of the eukaryotic replicative CMG helicase and pumpjack motion Deposited 2015-11-24 | Assembly 1 Protein heterocomplex Heteromer；Protein × 11 PDB declaration: undecameric（11） Consistent with protein count |
Chain 4
1–933（933 aa）
|
Not recorded | ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
20 mM Tris acetate, pH 7.5, 40 mM potassium glutamate, 2 mM DTT, 0.1 mM EDTA；pH 7.5；20 mM Tris acetate, pH 7.5, 40 mM potassium glutamate, 2 mM DTT, 0.1 mM EDTA
cryo-EM vitrification conditions
Blot for 3 seconds before plunging；Cryogen ETHANE；Blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK IV).
|
Resolution 4.80 Å |
| 5BK4 Cryo-EM structure of Mcm2-7 double hexamer on dsDNA Deposited 2017-09-12 | Assembly 1 Protein–DNA Heteromer；Protein × 12 PDB declaration: tetradecameric（14） Consistent with all polymers |
Chain 4
1–933（933 aa）
Chain C
1–933（933 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.90 Å |
| 5U8S Structure of eukaryotic CMG helicase at a replication fork Deposited 2016-12-14 | Assembly 1 Protein–DNA Heteromer；Protein × 11 PDB declaration: tridecameric（13） Consistent with all polymers |
Chain 4
1–933（933 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 3 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.10 Å R-free 0.391 |
| 5U8T Structure of Eukaryotic CMG Helicase at a Replication Fork and Implications Deposited 2016-12-15 | Assembly 1 Protein–DNA Heteromer；Protein × 11 PDB declaration: dodecameric（12） Consistent with all polymers |
Chain 4
1–933（933 aa）
|
Not recorded | ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 3 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.90 Å R-free 0.394 |
| 5V8F Structural basis of MCM2-7 replicative helicase loading by ORC-Cdc6 and Cdt1 Deposited 2017-03-21 | Assembly 1 Protein–DNA Heteromer；Protein × 14 PDB declaration: hexadecameric（16） Consistent with all polymers |
Chain 4
1–933（933 aa）
|
Not recorded | AGS PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.90 Å |
| 5XF8 Cryo-EM structure of the Cdt1-MCM2-7 complex in AMPPNP state Deposited 2017-04-09 | Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric（7） Consistent with protein count |
Chain 4
1–933（933 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 7.10 Å |
| 6EYC Re-refinement of the MCM2-7 double hexamer using ISOLDE Deposited 2017-11-11 | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain 4
1–933（933 aa）
|
Not recorded | ZN ZINC ION × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5；All details as per 3ja8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 6F0L S. cerevisiae MCM double hexamer bound to duplex DNA Deposited 2017-11-20 | Assembly 1 Protein–DNA Heteromer；Protein × 12 PDB declaration: tetradecameric（14） Consistent with all polymers |
Chain 4
1–933（933 aa）
Chain C
1–933（933 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 4 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.77 Å |
| 6HV9 S. cerevisiae CMG-Pol epsilon-DNA Deposited 2018-10-10 | Assembly 1 Protein–DNA Heteromer；Protein × 13 PDB declaration: hexadecameric（16） Consistent with all polymers |
Chain 4
1–933（933 aa）
|
Not recorded | AGS PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER × 2 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.98 Å |
| 6PTJ Structure of Ctf4 trimer in complex with one CMG helicase Deposited 2019-07-15 | Assembly 1 Protein heterocomplex Heteromer；Protein × 14 PDB declaration: tetradecameric（14） Consistent with protein count |
Chain 4
1–933（933 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 6PTN Structure of Ctf4 trimer in complex with two CMG helicases Deposited 2019-07-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 25 PDB declaration: 25-meric（25） Consistent with protein count |
Chain 4
1–933（933 aa）
Chain k
1–933（933 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.80 Å |
| 6PTO Structure of Ctf4 trimer in complex with three CMG helicases Deposited 2019-07-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 36 PDB declaration: 36-meric（36） Consistent with protein count |
Chain 4
1–933（933 aa）
Chain H
1–933（933 aa）
Chain j
1–933（933 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 7.00 Å |
| 6RQC Cryo-EM structure of an MCM loading intermediate Deposited 2019-05-15 | Assembly 1 Protein–DNA Heteromer；Protein × 12 PDB declaration: tetradecameric（14） Consistent with all polymers |
Chain 4
1–933（933 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 3 ADP ADENOSINE-5'-DIPHOSPHATE × 4 ZN ZINC ION × 5 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE；10 second incubation, 3.5 seconds single side blotting.
|
Resolution 4.40 Å |
| 6SKL Cryo-EM structure of the CMG Fork Protection Complex at a replication fork - Conformation 1 Deposited 2019-08-16 | Assembly 1 Protein–DNA Heteromer；Protein × 16 PDB declaration: octadecameric（18） Consistent with all polymers |
Chain 4
1–933（933 aa）
|
Not recorded | ZN ZINC ION × 5 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 3 MG MAGNESIUM ION × 3 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；Three microlitres of sample was applied on a grid and incubated for 15-30 s at 4 degC before manually blotting with filter paper for 10 s and plunge-freezing
in liquid ethane.
|
Resolution 3.70 Å |
| 6SKO Cryo-EM Structure of the Fork Protection Complex Bound to CMG at a Replication Fork - conformation 2 MCM CTD:ssDNA Deposited 2019-08-16 | Assembly 1 Protein–DNA Heteromer；Protein × 6 PDB declaration: heptameric（7） Consistent with all polymers |
Chain 4
1–933（933 aa）
Fragment：Mcm6-CTD
|
Not recorded | ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 5 MG MAGNESIUM ION × 5 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；Three microlitres of sample was applied on a grid and incubated for 15-30 s at 4 degC before manually blotting with filter paper for 10 s and plunge-freezing
in liquid ethane.
|
Resolution 3.40 Å |
| 6U0M Structure of the S. cerevisiae replicative helicase CMG in complex with a forked DNA Deposited 2019-08-14 | Assembly 1 Protein–DNA Heteromer；Protein × 11 PDB declaration: tridecameric（13） Consistent with all polymers |
Chain 4
177–929（753 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 3 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.90 Å |
| 6WGF Atomic model of mutant Mcm2-7 hexamer with Mcm6 WHD truncation Deposited 2020-04-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain 4
1–933（933 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 7.70 Å |
| 6WGG Atomic model of pre-insertion mutant OCCM-DNA complex(ORC-Cdc6-Cdt1-Mcm2-7 with Mcm6 WHD truncation) Deposited 2020-04-05 | Assembly 1 Protein–DNA Heteromer；Protein × 14 PDB declaration: hexadecameric（16） Consistent with all polymers |
Chain 4
1–933（933 aa）
|
Not recorded | AGS PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER × 4 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 8.10 Å |
| 6WGI Atomic model of the mutant OCCM (ORC-Cdc6-Cdt1-Mcm2-7 with Mcm6 WHD truncation) loaded on DNA at 10.5 A resolution Deposited 2020-04-05 | Assembly 1 Protein–DNA Heteromer；Protein × 14 PDB declaration: hexadecameric（16） Consistent with all polymers |
Chain 4
1–933（933 aa）
|
Not recorded | AGS PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER × 4 MG MAGNESIUM ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 10.00 Å |
| 7P30 3.0 A resolution structure of a DNA-loaded MCM double hexamer Deposited 2021-07-06 | Assembly 1 Protein–DNA Heteromer；Protein × 12 PDB declaration: tetradecameric（14） Consistent with all polymers |
Chain 4
1–933（933 aa）
Chain C
1–933（933 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 10 ZN ZINC ION × 10 ADP ADENOSINE-5'-DIPHOSPHATE × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE；blotted for 3 seconds before plunging
|
Resolution 3.00 Å |
| 7P5Z Structure of a DNA-loaded MCM double hexamer engaged with the Dbf4-dependent kinase Deposited 2021-07-15 | Assembly 1 Protein–DNA Heteromer；Protein × 14 PDB declaration: hexadecameric（16） Consistent with all polymers |
Chain 4
1–933（933 aa）
Chain C
1–933（933 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 10 ZN ZINC ION × 11 ADP ADENOSINE-5'-DIPHOSPHATE × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE；blotted for 3 seconds before plunging
|
Resolution 3.30 Å |
| 7PMK S. cerevisiae replisome-SCF(Dia2) complex bound to double-stranded DNA (conformation I) Deposited 2021-09-02 | Assembly 1 Protein–DNA Heteromer；Protein × 20 PDB declaration: 22-meric（22） Consistent with all polymers |
Chain 4
1–933（933 aa）
|
Not recorded | ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 3 MG MAGNESIUM ION × 3 ZN ZINC ION × 7 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE；Manual plunger
|
Resolution 3.20 Å |
| 7PMN S. cerevisiae replisome-SCF(Dia2) complex bound to double-stranded DNA (conformation II) Deposited 2021-09-02 | Assembly 1 Protein–DNA Heteromer；Protein × 20 PDB declaration: 22-meric（22） Consistent with all polymers |
Chain 4
1–933（933 aa）
|
Not recorded | ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 2 MG MAGNESIUM ION × 2 ZN ZINC ION × 7 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE；Manual plunger
|
Resolution 3.20 Å |
| 7PT6 Structure of MCM2-7 DH complexed with Cdc7-Dbf4 in the presence of ATPgS, state III Deposited 2021-09-26 | Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric（18） Consistent with protein count |
Chain 4
1–933（933 aa）
Chain D
1–933（933 aa）
|
Not recorded | AGS PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER × 12 MG MAGNESIUM ION × 14 ZN ZINC ION × 14 ADP ADENOSINE-5'-DIPHOSPHATE × 4 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；blot for 1.5 seconds and blot force +2
|
Resolution 3.20 Å |
| 7PT7 Structure of MCM2-7 DH complexed with Cdc7-Dbf4 in the presence of ADP:BeF3, state I Deposited 2021-09-26 | Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric（15） Consistent with protein count |
Chain 4
1–933（933 aa）
Chain D
1–933（933 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 13 MG MAGNESIUM ION × 14 ZN ZINC ION × 12 BEF BERYLLIUM TRIFLUORIDE ION × 7 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；blot for 1.5 seconds and blot force +2
|
Resolution 3.80 Å |
| 7QHS S. cerevisiae CMGE nucleating origin DNA melting Deposited 2021-12-14 | Assembly 1 Protein–DNA Heteromer；Protein × 13 PDB declaration: pentadecameric（15） Consistent with all polymers |
Chain 4
1–933（933 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 ZN ZINC ION × 7 MG MAGNESIUM ION × 3 ADP ADENOSINE-5'-DIPHOSPHATE × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 7V3U Cryo-EM structure of MCM double hexamer with structured Mcm4-NSD Deposited 2021-08-11 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain 4
1–933（933 aa）
Chain D
1–933（933 aa）
|
Not recorded | AGS PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER × 10 MG MAGNESIUM ION × 12 ZN ZINC ION × 10 ADP ADENOSINE-5'-DIPHOSPHATE × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 7V3V Cryo-EM structure of MCM double hexamer bound with DDK in State I Deposited 2021-08-11 | Assembly 1 Protein heterocomplex Heteromer；Protein × 14 PDB declaration: tetradecameric（14） Consistent with protein count |
Chain 4
1–933（933 aa）
Chain D
1–933（933 aa）
|
Not recorded | AGS PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER × 11 MG MAGNESIUM ION × 12 ZN ZINC ION × 11 ADP ADENOSINE-5'-DIPHOSPHATE × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 7W8G Cryo-EM structure of MCM double hexamer Deposited 2021-12-07 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain 4
1–933（933 aa）
Chain D
1–933（933 aa）
|
Not recorded | AGS PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER × 10 MG MAGNESIUM ION × 12 ZN ZINC ION × 10 ADP ADENOSINE-5'-DIPHOSPHATE × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.52 Å |
| 7Z13 S. cerevisiae CMGE dimer nucleating origin DNA melting Deposited 2022-02-24 | Assembly 1 Protein–DNA Heteromer；Protein × 26 PDB declaration: 28-meric（28） Consistent with all polymers |
Chain 4
1–933（933 aa）
Chain c
1–933（933 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 8 ZN ZINC ION × 14 MG MAGNESIUM ION × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 4 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 8B9A S. cerevisiae replisome + Ctf4, bound by pol alpha primase. Complex engaged with a fork DNA substrate containing a 60 nucleotide lagging strand. Deposited 2022-10-05 | Assembly 1 Protein–DNA Heteromer；Protein × 21 PDB declaration: 23-meric（23） Consistent with all polymers |
Chain 4
1–933（933 aa）
|
Not recorded | ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 4 MG MAGNESIUM ION × 4 ZN ZINC ION × 4 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 8B9B S. cerevisiae replisome + Ctf4, bound by pol alpha. Complex engaged with a fork DNA substrate containing a 60 nucleotide lagging strand. Deposited 2022-10-05 | Assembly 1 Protein–DNA Heteromer；Protein × 21 PDB declaration: 23-meric（23） Consistent with all polymers |
Chain 4
1–933（933 aa）
|
Not recorded | ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 4 MG MAGNESIUM ION × 4 ZN ZINC ION × 4 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 8B9C S. cerevisiae pol alpha - replisome complex Deposited 2022-10-05 | Assembly 1 Protein–DNA Heteromer；Protein × 18 PDB declaration: eicosameric（20） Consistent with all polymers |
Chain 4
1–933（933 aa）
|
Not recorded | ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 4 MG MAGNESIUM ION × 4 ZN ZINC ION × 4 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.60 Å |
| 8KG6 Yeast replisome in state I Deposited 2023-08-17 | Assembly 1 Protein–DNA Heteromer；Protein × 18 PDB declaration: 20-meric（20） Consistent with all polymers |
Chain 4
1–933（933 aa）
|
Not recorded | ZN ZINC ION × 7 ADP ADENOSINE-5'-DIPHOSPHATE × 2 MG MAGNESIUM ION × 5 AGS PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER × 3 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE；blot with filter paper for 3-4 seconds before plunging.
|
Resolution 3.07 Å |
| 8KG8 Yeast replisome in state II Deposited 2023-08-17 | Assembly 1 Protein–DNA Heteromer；Protein × 16 PDB declaration: octadecameric（18） Consistent with all polymers |
Chain 4
1–933（933 aa）
|
Not recorded | ZN ZINC ION × 7 ADP ADENOSINE-5'-DIPHOSPHATE × 2 MG MAGNESIUM ION × 5 AGS PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER × 3 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE；blot with filter paper for 3-4 seconds before plunging.
|
Resolution 4.23 Å |
| 8KG9 Yeast replisome in state III Deposited 2023-08-17 | Assembly 1 Protein–DNA Heteromer；Protein × 16 PDB declaration: octadecameric（18） Consistent with all polymers |
Chain 4
1–933（933 aa）
|
Not recorded | ZN ZINC ION × 7 ADP ADENOSINE-5'-DIPHOSPHATE × 2 MG MAGNESIUM ION × 4 AGS PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE；blot with filter paper for 3-4 seconds before plunging.
|
Resolution 4.52 Å |
| 8P5E S. cerevisiae nexus-sCMGE after DNA replication initiation Deposited 2023-05-24 | Assembly 1 Protein–DNA Heteromer；Protein × 13 PDB declaration: 15-meric（15） Consistent with all polymers |
Chain 4
1–933（933 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 ZN ZINC ION × 7 MG MAGNESIUM ION × 3 ADP ADENOSINE-5'-DIPHOSPHATE × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.90 Å |
| 8P62 S. cerevisiae ssDNA-sCMGE after DNA replication initiation Deposited 2023-05-25 | Assembly 1 Protein–DNA Heteromer；Protein × 13 PDB declaration: 14-meric（14） Consistent with all polymers |
Chain 4
1–933（933 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 ZN ZINC ION × 7 MG MAGNESIUM ION × 3 ADP ADENOSINE-5'-DIPHOSPHATE × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.90 Å |
| 8P63 S. cerevisiae consensus-sCMGE on ssDNA after DNA replication initiation Deposited 2023-05-25 | Assembly 1 Protein–DNA Heteromer；Protein × 13 PDB declaration: 14-meric（14） Consistent with all polymers |
Chain 4
1–933（933 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 ZN ZINC ION × 7 MG MAGNESIUM ION × 3 ADP ADENOSINE-5'-DIPHOSPHATE × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 8RIF Cryo-EM structure of the MCM double hexamer loaded onto dsDNA. Deposited 2023-12-18 | Assembly 1 Protein–DNA Heteromer；Protein × 12 PDB declaration: 14-meric（14） Consistent with all polymers |
Chain 4
1–933（933 aa）
Chain C
1–933（933 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 8 ZN ZINC ION × 10 ADP ADENOSINE-5'-DIPHOSPHATE × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.79 Å |
| 8RIG Cryo-EM structure of an MCM helicase single hexamer loaded onto dsDNA. Deposited 2023-12-18 | Assembly 1 Protein–DNA Heteromer；Protein × 6 PDB declaration: octameric（8） Consistent with all polymers |
Chain 4
1–933（933 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 6 ZN ZINC ION × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 4 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.41 Å |
| 8W7M Yeast replisome in state V Deposited 2023-08-30 | Assembly 1 Protein–DNA Heteromer；Protein × 15 PDB declaration: hexadecameric（16） Consistent with all polymers |
Chain 4
1–933（933 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 2 MG MAGNESIUM ION × 4 ZN ZINC ION × 5 AGS PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE；blot with filter paper for 3-4 seconds before plunging.
|
Resolution 4.12 Å |
| 8W7S Yeast replisome in state IV Deposited 2023-08-31 | Assembly 1 Protein–DNA Heteromer；Protein × 15 PDB declaration: hexadecameric（16） Consistent with all polymers |
Chain 4
1–933（933 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE；blot with filter paper for 3-4 seconds before plunging.
|
Resolution 7.39 Å |
| 8XGC Structure of yeast replisome associated with FACT and histone hexamer, Composite map Deposited 2023-12-15 | Assembly 1 Protein–DNA Heteromer；Protein × 27 PDB declaration: 29-meric（29） Consistent with all polymers |
Chain 4
1–933（933 aa）
|
Not recorded | ZN ZINC ION × 7 ADP ADENOSINE-5'-DIPHOSPHATE × 5 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 9E2W Cryo-EM structure of yeast CMG helicase stalled at G4-containing DNA template, state 1 Deposited 2024-10-23 | Assembly 1 Protein–DNA Heteromer；Protein × 13 PDB declaration: 15-meric（15） Consistent with all polymers |
Chain 4
1–933（933 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 5 ATP ADENOSINE-5'-TRIPHOSPHATE × 5 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE；Wait time 30 seconds after sample application; blot time 30 seconds with blot force 0
|
Resolution 3.30 Å |
| 9E2X Cryo-EM structure of yeast CMG helicase stalled at G4-containing DNA template, state 2 Deposited 2024-10-23 | Assembly 1 Protein–DNA Heteromer；Protein × 13 PDB declaration: 15-meric（15） Consistent with all polymers |
Chain 4
1–933（933 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 5 ZN ZINC ION × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE；Wait time 30 seconds after sample application; blot time 30 seconds with blot force 0
|
Resolution 3.50 Å |
| 9E2Y Cryo-EM structure of yeast CMG helicase stalled at G4-containing DNA template, state 3 Deposited 2024-10-23 | Assembly 1 Protein–DNA Heteromer；Protein × 12 PDB declaration: 14-meric（14） Consistent with all polymers |
Chain 4
1–933（933 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 5 ATP ADENOSINE-5'-TRIPHOSPHATE × 5 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE；Wait time 30 seconds after sample application; blot time 30 seconds with blot force 0
|
Resolution 3.20 Å |
| 9GJP OCCM maturation intermediate stalled with an Arginine Finger mutation in Mcm5: Conformer 2 Deposited 2024-08-22 | Assembly 1 Protein–DNA Heteromer；Protein × 13 PDB declaration: 15-meric（15） Consistent with all polymers |
Chain 4
1–933（933 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 4 ZN ZINC ION × 5 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 9GJW OCCM maturation intermediate stalled with an Arginine Finger mutation in Mcm2 Deposited 2024-08-22 | Assembly 1 Protein–DNA Heteromer；Protein × 13 PDB declaration: 15-meric（15） Consistent with all polymers |
Chain 4
1–933（933 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 6 ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 3 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 9GM5 OCCM maturation intermediate stalled with an Arginine Finger mutation in Mcm5: Conformer 1 Deposited 2024-08-28 | Assembly 1 Protein–DNA Heteromer；Protein × 13 PDB declaration: 15-meric（15） Consistent with all polymers |
Chain 4
1–933（933 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 4 ZN ZINC ION × 5 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 9I3I Cryo-EM structure of the MCM-ORC (MO) complex featuring an ORC2 regulatory domain involved in cell cycle regulation of MCM-DH loading for DNA replication. Deposited 2025-01-23 | Assembly 1 Protein–DNA Heteromer；Protein × 12 PDB declaration: 14-meric（14） Consistent with all polymers |
Chain 4
1–933（933 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 3 ADP ADENOSINE-5'-DIPHOSPHATE × 4 ZN ZINC ION × 5 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE；10 second incubation, 3.5 seconds single side blotting.
|
Resolution 4.40 Å |
| 9RHI pre-Initiation Complex on ARS1 DNA (monomer) Deposited 2025-06-09 | Assembly 1 Protein–DNA Heteromer；Protein × 17 PDB declaration: 19-meric（19） Consistent with all polymers |
Chain 4
1–933（933 aa）
|
Not recorded | ZN ZINC ION × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 9RHJ Pre-Initiation Complex on ARS1 DNA (dimer) Deposited 2025-06-09 | Assembly 1 Protein–DNA Heteromer；Protein × 30 PDB declaration: 32-meric（32） Consistent with all polymers |
Chain 4
1–933（933 aa）
Chain c
1–933（933 aa）
|
Not recorded | ZN ZINC ION × 10 ADP ADENOSINE-5'-DIPHOSPHATE × 2 MG MAGNESIUM ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 9RHL Phospho-DH bound by Sld3-MBD on ARS1 DNA Deposited 2025-06-09 | Assembly 1 Protein–DNA Heteromer；Protein × 14 PDB declaration: 16-meric（16） Consistent with all polymers |
Chain 4
1–933（933 aa）
Chain c
1–933（933 aa）
|
Not recorded | ZN ZINC ION × 10 ADP ADENOSINE-5'-DIPHOSPHATE × 4 MG MAGNESIUM ION × 4 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 9RHM Phospho-MCM double hexamer bound to Sld3-Sld7-Cdc45 on ARS1 DNA Deposited 2025-06-09 | Assembly 1 Protein–DNA Heteromer；Protein × 17 PDB declaration: 19-meric（19） Consistent with all polymers |
Chain 4
1–933（933 aa）
Chain c
1–933（933 aa）
|
Not recorded | ZN ZINC ION × 10 ADP ADENOSINE-5'-DIPHOSPHATE × 4 MG MAGNESIUM ION × 4 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |