Current Protein Identity：P38398
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1JM7 Solution structure of the BRCA1/BARD1 RING-domain heterodimer Deposited 2001-07-17 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–110（110 aa）
Fragment：RING-Domain
|
Not recorded | ZN ZINC ION × 4 | SOLUTION NMR |
NMR measurement conditions
pH 6.8；315 K；Ionic strength (raw mmCIF value) 0.2M NaCl；Pressure ambient
NMR measurement conditions
pH 6.8；315 K；Ionic strength (raw mmCIF value) 0.2M NaCl；Pressure ambient
NMR measurement conditions
pH 6.8；315 K；Ionic strength (raw mmCIF value) 0.2M NaCl；Pressure ambient
NMR measurement conditions
pH 6.8；315 K；Ionic strength (raw mmCIF value) 0.2M NaCl；Pressure ambient
NMR sample composition
sample 1: 0.8-1.1mM U-15N,13C BRCA1; unlabeled BARD1,
25mM sodium phosphate,
0.2M NaCl,
2mM DTT.
Sample 2:
0.8-1.1mM U-15N,13C BARD1; unlabeled BRCA1,
25mM sodium phosphate,
0.2M NaCl,
2mM DTT.
Sample 3:
0.8-1.1mM U-15N,13C,85%-2H BRCA1; unlabeled BARD1,
25mM sodium phosphate,
0.2M NaCl,
2mM DTT.
SAMPLE 4:
0.8-1.1mM U-15N,13C,85%-2H BARD1; unlabeled BRCA,1
25mM sodium phosphate,
0.2M NaCl,
2mM DTT. | 90% H2O/10% D2O
NMR sample composition
0.8-1.1mM U-15N,13C BARD1; unlabeled BRCA1
25mM sodium phosphate
0.2M NaCl
2mM DTT | 90% H2O/10% D2O
NMR sample composition
0.8-1.1mM U-15N,13C,85%-2H BRCA1; unlabeled BARD1
25mM sodium phosphate
0.2M NaCl
2mM DTT | 90% H2O/10% D2O
NMR sample composition
0.8-1.1mM U-15N,13C,85%-2H BARD1; unlabeled BRCA1
25mM sodium phosphate
0.2M NaCl
2mM DTT | 90% H2O/10% D2O
|
Resolution not provided |
| 1JNX Crystal structure of the BRCT repeat region from the breast cancer associated protein, BRCA1 Deposited 2001-07-26 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain X
1646–1859（214 aa）
Fragment：residues 1646-1859
|
Not recorded | NI NICKEL (II) ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 8.5；298 K；Lithium sulphate, Nickel sulphate, calcium chloride, Tris, pH 8.5,VAPOR DIFFUSION, HANGING DROP at 298K
|
Resolution 2.50 Å R-free 0.302 |
| 1N5O Structural consequences of a cancer-causing BRCA1-BRCT missense mutation Deposited 2002-11-06 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain X
1646–1859（214 aa）
Fragment：BRCT (residues 1646-1859)
|
Mutation：M1775R | CO COBALT (II) ION × 1 SO4 SULFATE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.7；293 K；ammonium sulphate, cobalt chloride, MES, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.80 Å R-free 0.298 |
| 1OQA Solution structure of the BRCT-c domain from human BRCA1 Deposited 2003-03-07 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1755–1863（109 aa）
Fragment：BRCT-c
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 6.8；295.9 K；Pressure 1
NMR sample composition
0.7mM BRCT-c; U-15N,13C, 20mM Potassium phosphate buffer pH 6.8, 5 mM KCl, 10 mM d10-DTT, 99.98% D2O | 99.98% D2O
NMR sample composition
1.3 mM BRCT-c; U-15N, 20 mM Potassium phosphate buffer pH 6.8, 5 mM KCl, 10 mM d10-DTT, 93% H2O, 7% D2O | 93% H2O/7% D2O
NMR sample composition
1.3 mM BRCT-c; U-15N, 20 mM Potassium phosphate buffer pH 6.8, 5 mM KCl, 10 mM d10-DTT, 99.98% D2O | 99.98% D2O
|
Resolution not provided |
| 1T15 Crystal Structure of the Brca1 BRCT Domains in Complex with the Phosphorylated Interacting Region from Bach1 Helicase Deposited 2004-04-15 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1649–1859（211 aa）
Fragment：BCRT 1, BCRT 2
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
MICROBATCH；pH 6.5；291 K；PEG 8000, Ammonium Sulphate, MES, pH 6.5, Microbatch, temperature 291K
|
Resolution 1.85 Å R-free 0.222 |
| 1T29 Crystal structure of the BRCA1 BRCT repeats bound to a phosphorylated BACH1 peptide Deposited 2004-04-20 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1646–1859（214 aa）
Fragment：BRCT repeats of BRCA1 (residues 1646-1859)
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；295 K；PEG4000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 2.30 Å R-free 0.266 |
| 1T2U Structural basis of phosphopeptide recognition by the BRCT domain of BRCA1: structure of BRCA1 missense variant V1809F Deposited 2004-04-22 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1646–1859（214 aa）
Fragment：BRCT DOMAIN 1646-1859
|
Mutation：V1809F | CO COBALT (II) ION × 1 SO4 SULFATE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.7；298 K；Ammounium sulphate, cobaltous chloride, MES, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 6.70
|
Resolution 2.80 Å R-free 0.294 |
| 1T2V Structural basis of phospho-peptide recognition by the BRCT domain of BRCA1, structure with phosphopeptide Deposited 2004-04-22 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1646–1859（214 aa）
Fragment：BRCT DOMAIN 1646-1859
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 5.6；298 K；PEG 4000 ammonium acetate, tri-sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 5.60
|
Resolution 3.30 Å R-free 0.301 |
| 1T2V Structural basis of phospho-peptide recognition by the BRCT domain of BRCA1, structure with phosphopeptide Deposited 2004-04-22 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
1646–1859（214 aa）
Fragment：BRCT DOMAIN 1646-1859
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 5.6；298 K；PEG 4000 ammonium acetate, tri-sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 5.60
|
Resolution 3.30 Å R-free 0.301 |
| 1T2V Structural basis of phospho-peptide recognition by the BRCT domain of BRCA1, structure with phosphopeptide Deposited 2004-04-22 | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain C
1646–1859（214 aa）
Fragment：BRCT DOMAIN 1646-1859
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 5.6；298 K；PEG 4000 ammonium acetate, tri-sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 5.60
|
Resolution 3.30 Å R-free 0.301 |
| 1T2V Structural basis of phospho-peptide recognition by the BRCT domain of BRCA1, structure with phosphopeptide Deposited 2004-04-22 | Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain D
1646–1859（214 aa）
Fragment：BRCT DOMAIN 1646-1859
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 5.6；298 K；PEG 4000 ammonium acetate, tri-sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 5.60
|
Resolution 3.30 Å R-free 0.301 |
| 1T2V Structural basis of phospho-peptide recognition by the BRCT domain of BRCA1, structure with phosphopeptide Deposited 2004-04-22 | Assembly 5 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain E
1646–1859（214 aa）
Fragment：BRCT DOMAIN 1646-1859
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 5.6；298 K；PEG 4000 ammonium acetate, tri-sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 5.60
|
Resolution 3.30 Å R-free 0.301 |
| 1T2V Structural basis of phospho-peptide recognition by the BRCT domain of BRCA1, structure with phosphopeptide Deposited 2004-04-22 | Assembly 6 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1646–1859（214 aa）
Fragment：BRCT DOMAIN 1646-1859
Chain D
1646–1859（214 aa）
Fragment：BRCT DOMAIN 1646-1859
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 5.6；298 K；PEG 4000 ammonium acetate, tri-sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 5.60
|
Resolution 3.30 Å R-free 0.301 |
| 1T2V Structural basis of phospho-peptide recognition by the BRCT domain of BRCA1, structure with phosphopeptide Deposited 2004-04-22 | Assembly 7 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain E
1646–1859（214 aa）
Fragment：BRCT DOMAIN 1646-1859
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 5.6；298 K；PEG 4000 ammonium acetate, tri-sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 5.60
|
Resolution 3.30 Å R-free 0.301 |
| 1Y98 Structure of the BRCT repeats of BRCA1 bound to a CtIP phosphopeptide. Deposited 2004-12-14 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1646–1859（214 aa）
Fragment：BRCT 1, BRCT 2
|
Not recorded | CO COBALT (II) ION × 1 SO4 SULFATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；AMMONIUM SULPHATE, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 2.50 Å R-free 0.270 |
| 1Y98 Structure of the BRCT repeats of BRCA1 bound to a CtIP phosphopeptide. Deposited 2004-12-14 | Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1646–1859（214 aa）
Fragment：BRCT 1, BRCT 2
|
Not recorded | CO COBALT (II) ION × 2 SO4 SULFATE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；AMMONIUM SULPHATE, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 2.50 Å R-free 0.270 |
| 2ING X-ray Structure of the BRCA1 BRCT mutant M1775K Deposited 2006-10-07 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain X
1649–1859（211 aa）
Fragment：BRCT1, BRCT2
|
Mutation：M1775K | CO COBALT (II) ION × 1 SO4 SULFATE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.7；291 K；1.4M Ammonium sulfate, 20mM Cobalt chloride, 100mM 2-(N-Morpholino) ethanesulfonic acid, pH 6.7, VAPOR DIFFUSION, SITTING DROP, temperature 291K
|
Resolution 3.60 Å R-free 0.302 |
| 2ING X-ray Structure of the BRCA1 BRCT mutant M1775K Deposited 2006-10-07 | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain X
1649–1859（211 aa）
Fragment：BRCT1, BRCT2
|
Mutation：M1775K | CO COBALT (II) ION × 2 SO4 SULFATE ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.7；291 K；1.4M Ammonium sulfate, 20mM Cobalt chloride, 100mM 2-(N-Morpholino) ethanesulfonic acid, pH 6.7, VAPOR DIFFUSION, SITTING DROP, temperature 291K
|
Resolution 3.60 Å R-free 0.302 |
| 3COJ Crystal Structure of the BRCT Domains of Human BRCA1 in Complex with a Phosphorylated Peptide from Human Acetyl-CoA Carboxylase 1 Deposited 2008-03-28 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain X
1646–1859（214 aa）
Fragment：BRCT1 and BRCT2 domains
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 4.5；294 K；100 mM sodium acetate (pH 4.5), 25-27% (v/v) PEG400, and 200 mM calcium acetate, VAPOR DIFFUSION, SITTING DROP, temperature 294K
|
Resolution 3.21 Å R-free 0.307 |
| 3COJ Crystal Structure of the BRCT Domains of Human BRCA1 in Complex with a Phosphorylated Peptide from Human Acetyl-CoA Carboxylase 1 Deposited 2008-03-28 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1646–1859（214 aa）
Fragment：BRCT1 and BRCT2 domains
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 4.5；294 K；100 mM sodium acetate (pH 4.5), 25-27% (v/v) PEG400, and 200 mM calcium acetate, VAPOR DIFFUSION, SITTING DROP, temperature 294K
|
Resolution 3.21 Å R-free 0.307 |
| 3COJ Crystal Structure of the BRCT Domains of Human BRCA1 in Complex with a Phosphorylated Peptide from Human Acetyl-CoA Carboxylase 1 Deposited 2008-03-28 | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
1646–1859（214 aa）
Fragment：BRCT1 and BRCT2 domains
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 4.5；294 K；100 mM sodium acetate (pH 4.5), 25-27% (v/v) PEG400, and 200 mM calcium acetate, VAPOR DIFFUSION, SITTING DROP, temperature 294K
|
Resolution 3.21 Å R-free 0.307 |
| 3COJ Crystal Structure of the BRCT Domains of Human BRCA1 in Complex with a Phosphorylated Peptide from Human Acetyl-CoA Carboxylase 1 Deposited 2008-03-28 | Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain C
1646–1859（214 aa）
Fragment：BRCT1 and BRCT2 domains
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 4.5；294 K；100 mM sodium acetate (pH 4.5), 25-27% (v/v) PEG400, and 200 mM calcium acetate, VAPOR DIFFUSION, SITTING DROP, temperature 294K
|
Resolution 3.21 Å R-free 0.307 |
| 3COJ Crystal Structure of the BRCT Domains of Human BRCA1 in Complex with a Phosphorylated Peptide from Human Acetyl-CoA Carboxylase 1 Deposited 2008-03-28 | Assembly 5 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain D
1646–1859（214 aa）
Fragment：BRCT1 and BRCT2 domains
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 4.5；294 K；100 mM sodium acetate (pH 4.5), 25-27% (v/v) PEG400, and 200 mM calcium acetate, VAPOR DIFFUSION, SITTING DROP, temperature 294K
|
Resolution 3.21 Å R-free 0.307 |
| 3COJ Crystal Structure of the BRCT Domains of Human BRCA1 in Complex with a Phosphorylated Peptide from Human Acetyl-CoA Carboxylase 1 Deposited 2008-03-28 | Assembly 6 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain E
1646–1859（214 aa）
Fragment：BRCT1 and BRCT2 domains
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 4.5；294 K；100 mM sodium acetate (pH 4.5), 25-27% (v/v) PEG400, and 200 mM calcium acetate, VAPOR DIFFUSION, SITTING DROP, temperature 294K
|
Resolution 3.21 Å R-free 0.307 |
| 3COJ Crystal Structure of the BRCT Domains of Human BRCA1 in Complex with a Phosphorylated Peptide from Human Acetyl-CoA Carboxylase 1 Deposited 2008-03-28 | Assembly 7 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain F
1646–1859（214 aa）
Fragment：BRCT1 and BRCT2 domains
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 4.5；294 K；100 mM sodium acetate (pH 4.5), 25-27% (v/v) PEG400, and 200 mM calcium acetate, VAPOR DIFFUSION, SITTING DROP, temperature 294K
|
Resolution 3.21 Å R-free 0.307 |
| 3COJ Crystal Structure of the BRCT Domains of Human BRCA1 in Complex with a Phosphorylated Peptide from Human Acetyl-CoA Carboxylase 1 Deposited 2008-03-28 | Assembly 8 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain G
1646–1859（214 aa）
Fragment：BRCT1 and BRCT2 domains
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 4.5；294 K；100 mM sodium acetate (pH 4.5), 25-27% (v/v) PEG400, and 200 mM calcium acetate, VAPOR DIFFUSION, SITTING DROP, temperature 294K
|
Resolution 3.21 Å R-free 0.307 |
| 3K0H The crystal structure of BRCA1 BRCT in complex with a minimal recognition tetrapeptide with an amidated C-terminus Deposited 2009-09-24 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1646–1859（214 aa）
Fragment：BRCT Domain (UNP residues 1646 to 1859)
|
Not recorded | NI NICKEL (II) ION × 1 CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.3；298 K；Tris-HCl, Li2SO4, NiCl2, pH 7.3, VAPOR DIFFUSION, temperature 298K
|
Resolution 2.70 Å R-free 0.296 |
| 3K0K Crystal Structure of BRCA1 BRCT in complex with a minimal recognition tetrapeptide with a free carboxy C-terminus. Deposited 2009-09-24 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1646–1859（214 aa）
Fragment：BRCT Domain (UNP residues 1646 to 1859)
|
Not recorded | NI NICKEL (II) ION × 1 CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.3；298 K；Li2SO4, Tris-HCl, NiCl2, pH 7.3, VAPOR DIFFUSION, temperature 298K
|
Resolution 2.70 Å R-free 0.299 |
| 3K15 Crystal Structure of BRCA1 BRCT D1840T in complex with a minimal recognition tetrapeptide with an amidated C-terminus Deposited 2009-09-25 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1646–1859（214 aa）
Fragment：BRCT Domain (UNP residues 1646 to 1859)
|
Mutation：D1840T | NI NICKEL (II) ION × 1 CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 8.5；298 K；Li2SO4, Tris-HCl, NiCl2, pH 8.5, VAPOR DIFFUSION, temperature 298K
|
Resolution 2.80 Å R-free 0.280 |
| 3K16 Crystal Structure of BRCA1 BRCT D1840T in complex with a minimal recognition tetrapeptide with a free carboxy C-terminus Deposited 2009-09-25 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1646–1859（214 aa）
Fragment：BRCT Domain
|
Mutation：D1840T | NI NICKEL (II) ION × 1 CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 8.5；298 K；Li2SO4, Tris-HCl, NiCl2, pH 8.5, VAPOR DIFFUSION, temperature 298K
|
Resolution 3.00 Å R-free 0.288 |
| 3PXA Impact of BRCA1 BRCT domain missense substitutions on phospho-peptide recognition: G1656D Deposited 2010-12-09 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1646–1859（214 aa）
Fragment：BRCT Domain, UNP residues 1646-1859
|
Mutation：G1656D | NI NICKEL (II) ION × 1 SO4 SULFATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；1.0M Li2SO4
100mM Tris
5mM CaCl2
10mM NiCl2
, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.55 Å R-free 0.261 |
| 3PXB Impact of BRCA1 BRCT domain missense substitutions on phospho-peptide recognition: T1700A Deposited 2010-12-09 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1646–1859（214 aa）
Fragment：BRCT domain, UNP residues 1646-1859
|
Mutation：T1700A | SO4 SULFATE ION × 1 NI NICKEL (II) ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；293 K；0.8 M Li2SO4
100 mM TRIS
5mM CaCl2
10 mM NiCl2, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.50 Å R-free 0.274 |
| 3PXC Impact of BRCA1 BRCT domain missense substitutions on phospho-peptide recognition: R1699Q Deposited 2010-12-09 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain X
1646–1859（214 aa）
Fragment：BRCT domain, UNP residues 1646-1859
|
Mutation：R1699Q | SO4 SULFATE ION × 1 NI NICKEL (II) ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；0.8M Li2SO4
100mM Tris
5mM CaCl2
10mM NiCl2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.80 Å R-free 0.270 |
| 3PXD Impact of BRCA1 BRCT domain missense substitutions on phospho-peptide recognition: R1835P Deposited 2010-12-09 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1646–1859（214 aa）
Fragment：BRCT Domain, UNP residues 1646-1859
|
Mutation：R1835P | SO4 SULFATE ION × 1 NI NICKEL (II) ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；0.8M Li2SO4
100 mM Tris
5mM CaCl2
10mM NiCl2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.80 Å R-free 0.281 |
| 3PXE Impact of BRCA1 BRCT domain missense substitutions on phospho-peptide recognition: E1836K Deposited 2010-12-09 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1646–1859（214 aa）
Fragment：BRCT domain, UNP residues 1646-1859
|
Mutation：E1836K | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；50mM MES
0.15M (NH4)2SO4
26% PEG 8000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.85 Å R-free 0.266 |
| 3PXE Impact of BRCA1 BRCT domain missense substitutions on phospho-peptide recognition: E1836K Deposited 2010-12-09 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
1646–1859（214 aa）
Fragment：BRCT domain, UNP residues 1646-1859
|
Mutation：E1836K | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；50mM MES
0.15M (NH4)2SO4
26% PEG 8000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.85 Å R-free 0.266 |
| 3PXE Impact of BRCA1 BRCT domain missense substitutions on phospho-peptide recognition: E1836K Deposited 2010-12-09 | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain C
1646–1859（214 aa）
Fragment：BRCT domain, UNP residues 1646-1859
|
Mutation：E1836K | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；50mM MES
0.15M (NH4)2SO4
26% PEG 8000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.85 Å R-free 0.266 |
| 3PXE Impact of BRCA1 BRCT domain missense substitutions on phospho-peptide recognition: E1836K Deposited 2010-12-09 | Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain D
1646–1859（214 aa）
Fragment：BRCT domain, UNP residues 1646-1859
|
Mutation：E1836K | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；50mM MES
0.15M (NH4)2SO4
26% PEG 8000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.85 Å R-free 0.266 |
| 4IFI Structure of human BRCA1 BRCT in complex with BAAT peptide Deposited 2012-12-14 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1646–1859（214 aa）
Fragment：BRCT domain, UNP residues 1650-1859
|
Not recorded | ACT ACETATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.8；293 K；30% PEG 6000, 1M LiCl. 0.1M Sodium Acetate , pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.20 Å R-free 0.261 |
| 4IFI Structure of human BRCA1 BRCT in complex with BAAT peptide Deposited 2012-12-14 | Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1646–1859（214 aa）
Fragment：BRCT domain, UNP residues 1650-1859
|
Not recorded | ACT ACETATE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.8；293 K；30% PEG 6000, 1M LiCl. 0.1M Sodium Acetate , pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.20 Å R-free 0.261 |
| 4IGK Structure of human BRCA1 BRCT in complex with ATRIP peptide Deposited 2012-12-17 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1646–1859（214 aa）
Fragment：BRCT domain, UNP residues 1646-1859
|
Not recorded | GOL GLYCEROL × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；20% PEG3350, 0.2 M ammonium acetate, 8% glycerol, 0.1 M HEPES pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.75 Å R-free 0.204 |
| 4IGK Structure of human BRCA1 BRCT in complex with ATRIP peptide Deposited 2012-12-17 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
1646–1859（214 aa）
Fragment：BRCT domain, UNP residues 1646-1859
|
Not recorded | GOL GLYCEROL × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；20% PEG3350, 0.2 M ammonium acetate, 8% glycerol, 0.1 M HEPES pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.75 Å R-free 0.204 |
| 4JLU Crystal structure of BRCA1 BRCT with doubly phosphorylated Abraxas Deposited 2013-03-13 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1649–1859（211 aa）
Fragment：UNP RESIDUES 1649-1859
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；pH 6.5, VAPOR DIFFUSION, HANGING DROP
|
Resolution 3.50 Å R-free 0.327 |
| 4OFB Crystal structure of human BRCA1 BRCT in complex with nonphosphopeptide inhibitor Deposited 2014-01-14 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1646–1859（214 aa）
Fragment：BRCT 1 domain (UNP residues 1646-1859)
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；298 K；0.1M Sodium Cacodylate, 0.1M Magnesium Acetate,24% PEG6000, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 3.05 Å R-free 0.237 |
| 4U4A Complex Structure of BRCA1 BRCT with singly phospho Abraxas Deposited 2014-07-23 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1646–1859（214 aa）
Fragment：BRCT (UNP RESIDUES 1646-1859)
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；295 K；0.01M Cobalt Chloride, 0.1 M(MES), pH 6.5, 30% PEG Mono Methyl Ether (MME) 5000
|
Resolution 3.51 Å R-free 0.362 |
| 4U4A Complex Structure of BRCA1 BRCT with singly phospho Abraxas Deposited 2014-07-23 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
1646–1859（214 aa）
Fragment：BRCT (UNP RESIDUES 1646-1859)
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；295 K；0.01M Cobalt Chloride, 0.1 M(MES), pH 6.5, 30% PEG Mono Methyl Ether (MME) 5000
|
Resolution 3.51 Å R-free 0.362 |
| 4U4A Complex Structure of BRCA1 BRCT with singly phospho Abraxas Deposited 2014-07-23 | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain C
1646–1859（214 aa）
Fragment：BRCT (UNP RESIDUES 1646-1859)
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；295 K；0.01M Cobalt Chloride, 0.1 M(MES), pH 6.5, 30% PEG Mono Methyl Ether (MME) 5000
|
Resolution 3.51 Å R-free 0.362 |
| 4Y18 Structure of BRCA1 BRCT domains in complex with Abraxas double phosphorylated peptide Deposited 2015-02-06 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1646–1859（214 aa）
Fragment：BRCT domains, UNP residues 1646-1859
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；291 K；HEPES, Ammonium Sulphate, PEG 4000.
|
Resolution 3.50 Å R-free 0.297 |
| 4Y18 Structure of BRCA1 BRCT domains in complex with Abraxas double phosphorylated peptide Deposited 2015-02-06 | Assembly 10 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain G
1646–1859（214 aa）
Fragment：BRCT domains, UNP residues 1646-1859
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；291 K；HEPES, Ammonium Sulphate, PEG 4000.
|
Resolution 3.50 Å R-free 0.297 |
| 4Y18 Structure of BRCA1 BRCT domains in complex with Abraxas double phosphorylated peptide Deposited 2015-02-06 | Assembly 11 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain G
1646–1859（214 aa）
Fragment：BRCT domains, UNP residues 1646-1859
Chain H
1646–1859（214 aa）
Fragment：BRCT domains, UNP residues 1646-1859
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；291 K；HEPES, Ammonium Sulphate, PEG 4000.
|
Resolution 3.50 Å R-free 0.297 |
| 4Y18 Structure of BRCA1 BRCT domains in complex with Abraxas double phosphorylated peptide Deposited 2015-02-06 | Assembly 12 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain H
1646–1859（214 aa）
Fragment：BRCT domains, UNP residues 1646-1859
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；291 K；HEPES, Ammonium Sulphate, PEG 4000.
|
Resolution 3.50 Å R-free 0.297 |
| 4Y18 Structure of BRCA1 BRCT domains in complex with Abraxas double phosphorylated peptide Deposited 2015-02-06 | Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1646–1859（214 aa）
Fragment：BRCT domains, UNP residues 1646-1859
Chain B
1646–1859（214 aa）
Fragment：BRCT domains, UNP residues 1646-1859
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；291 K；HEPES, Ammonium Sulphate, PEG 4000.
|
Resolution 3.50 Å R-free 0.297 |
| 4Y18 Structure of BRCA1 BRCT domains in complex with Abraxas double phosphorylated peptide Deposited 2015-02-06 | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
1646–1859（214 aa）
Fragment：BRCT domains, UNP residues 1646-1859
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；291 K；HEPES, Ammonium Sulphate, PEG 4000.
|
Resolution 3.50 Å R-free 0.297 |
| 4Y18 Structure of BRCA1 BRCT domains in complex with Abraxas double phosphorylated peptide Deposited 2015-02-06 | Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain C
1646–1859（214 aa）
Fragment：BRCT domains, UNP residues 1646-1859
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；291 K；HEPES, Ammonium Sulphate, PEG 4000.
|
Resolution 3.50 Å R-free 0.297 |
| 4Y18 Structure of BRCA1 BRCT domains in complex with Abraxas double phosphorylated peptide Deposited 2015-02-06 | Assembly 5 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain C
1646–1859（214 aa）
Fragment：BRCT domains, UNP residues 1646-1859
Chain E
1646–1859（214 aa）
Fragment：BRCT domains, UNP residues 1646-1859
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；291 K；HEPES, Ammonium Sulphate, PEG 4000.
|
Resolution 3.50 Å R-free 0.297 |
| 4Y18 Structure of BRCA1 BRCT domains in complex with Abraxas double phosphorylated peptide Deposited 2015-02-06 | Assembly 6 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain D
1646–1859（214 aa）
Fragment：BRCT domains, UNP residues 1646-1859
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；291 K；HEPES, Ammonium Sulphate, PEG 4000.
|
Resolution 3.50 Å R-free 0.297 |
| 4Y18 Structure of BRCA1 BRCT domains in complex with Abraxas double phosphorylated peptide Deposited 2015-02-06 | Assembly 7 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain D
1646–1859（214 aa）
Fragment：BRCT domains, UNP residues 1646-1859
Chain F
1646–1859（214 aa）
Fragment：BRCT domains, UNP residues 1646-1859
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；291 K；HEPES, Ammonium Sulphate, PEG 4000.
|
Resolution 3.50 Å R-free 0.297 |
| 4Y18 Structure of BRCA1 BRCT domains in complex with Abraxas double phosphorylated peptide Deposited 2015-02-06 | Assembly 8 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain E
1646–1859（214 aa）
Fragment：BRCT domains, UNP residues 1646-1859
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；291 K；HEPES, Ammonium Sulphate, PEG 4000.
|
Resolution 3.50 Å R-free 0.297 |
| 4Y18 Structure of BRCA1 BRCT domains in complex with Abraxas double phosphorylated peptide Deposited 2015-02-06 | Assembly 9 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain F
1646–1859（214 aa）
Fragment：BRCT domains, UNP residues 1646-1859
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；291 K；HEPES, Ammonium Sulphate, PEG 4000.
|
Resolution 3.50 Å R-free 0.297 |
| 4Y2G Structure of BRCA1 BRCT domains in complex with Abraxas single phosphorylated peptide Deposited 2015-02-09 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1646–1859（214 aa）
Fragment：UNP residues 1646-1859
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；291 K；Lithium chloride, Tris, PEG 6000
|
Resolution 2.50 Å R-free 0.252 |
| 6G2I Filament of acetyl-CoA carboxylase and BRCT domains of BRCA1 (ACC-BRCT) at 5.9 A resolution Deposited 2018-03-23 | Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric（18） Consistent with protein count |
Chain H
1667–1880（214 aa）
Chain K
1667–1880（214 aa）
Chain M
1667–1880（214 aa）
Chain O
1667–1880（214 aa）
Chain S
1667–1880（214 aa）
Chain U
1667–1880（214 aa）
Chain W
1667–1880（214 aa）
Chain Y
1667–1880（214 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.90 Å |
| 7LYB Cryo-EM structure of the human nucleosome core particle in complex with BRCA1-BARD1-UbcH5c Deposited 2021-03-06 | Assembly 1 Protein–DNA Heteromer；Protein × 11 PDB declaration: tridecameric（13） Consistent with all polymers |
Chain M
1–100（100 aa）
Fragment：RING domain (UNP residues 1-100)
|
Not recorded | ZN ZINC ION × 4 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.28 Å |
| 8GRQ Cryo-EM structure of BRCA1/BARD1 bound to H2AK127-UbcH5c-Ub nucleosome Deposited 2022-09-02 | Assembly 1 Protein–DNA Heteromer；Protein × 11 PDB declaration: tridecameric（13） Consistent with all polymers |
Chain K
6–97（92 aa）
|
Not recorded | ZN ZINC ION × 4 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.87 Å |
| 8RS8 Crystal structure of BRCA1 BRCTs in complex with a RIF1 phosphopeptide Deposited 2024-01-24 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1646–1859（214 aa）
|
Not recorded | EDO 1,2-ETHANEDIOL × 9 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；287 K；55.5 mM MES, 44.5 mM Imidazole, 120 mM Diethylene glycol, 120 mM Triethylene-glycol, 120 mM Tetraethylene glycol, 120 mM Pentaethylene glycol, 20% (v/v) Ethylene glycol, 10% (w/v) PEG 8000
|
Resolution 1.31 Å R-free 0.179 |
| 8RS8 Crystal structure of BRCA1 BRCTs in complex with a RIF1 phosphopeptide Deposited 2024-01-24 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
1646–1859（214 aa）
|
Not recorded | EDO 1,2-ETHANEDIOL × 7 IMD IMIDAZOLE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；287 K；55.5 mM MES, 44.5 mM Imidazole, 120 mM Diethylene glycol, 120 mM Triethylene-glycol, 120 mM Tetraethylene glycol, 120 mM Pentaethylene glycol, 20% (v/v) Ethylene glycol, 10% (w/v) PEG 8000
|
Resolution 1.31 Å R-free 0.179 |
| 8RS8 Crystal structure of BRCA1 BRCTs in complex with a RIF1 phosphopeptide Deposited 2024-01-24 | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain C
1646–1859（214 aa）
|
Not recorded | EDO 1,2-ETHANEDIOL × 13 IMD IMIDAZOLE × 1 PEG DI(HYDROXYETHYL)ETHER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；287 K；55.5 mM MES, 44.5 mM Imidazole, 120 mM Diethylene glycol, 120 mM Triethylene-glycol, 120 mM Tetraethylene glycol, 120 mM Pentaethylene glycol, 20% (v/v) Ethylene glycol, 10% (w/v) PEG 8000
|
Resolution 1.31 Å R-free 0.179 |
| 8RS8 Crystal structure of BRCA1 BRCTs in complex with a RIF1 phosphopeptide Deposited 2024-01-24 | Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain D
1646–1859（214 aa）
|
Not recorded | EDO 1,2-ETHANEDIOL × 8 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；287 K；55.5 mM MES, 44.5 mM Imidazole, 120 mM Diethylene glycol, 120 mM Triethylene-glycol, 120 mM Tetraethylene glycol, 120 mM Pentaethylene glycol, 20% (v/v) Ethylene glycol, 10% (w/v) PEG 8000
|
Resolution 1.31 Å R-free 0.179 |
| 9QPX BRCA1 BRCT tandem repeat with RNA polymerase II pSer5-CTD peptide Deposited 2025-03-30 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1646–1859（214 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6；277 K；0.1M PCB buffer (sodium propionate, sodium cacodylate, BIS-TRIS propane in the molar ratios 2:1:2) pH 6, 25%PEG 1500
|
Resolution 3.00 Å R-free 0.312 |
| 9QPX BRCA1 BRCT tandem repeat with RNA polymerase II pSer5-CTD peptide Deposited 2025-03-30 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain D
1646–1859（214 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6；277 K；0.1M PCB buffer (sodium propionate, sodium cacodylate, BIS-TRIS propane in the molar ratios 2:1:2) pH 6, 25%PEG 1500
|
Resolution 3.00 Å R-free 0.312 |
| 9QPX BRCA1 BRCT tandem repeat with RNA polymerase II pSer5-CTD peptide Deposited 2025-03-30 | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
1646–1859（214 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6；277 K；0.1M PCB buffer (sodium propionate, sodium cacodylate, BIS-TRIS propane in the molar ratios 2:1:2) pH 6, 25%PEG 1500
|
Resolution 3.00 Å R-free 0.312 |
| 9QPX BRCA1 BRCT tandem repeat with RNA polymerase II pSer5-CTD peptide Deposited 2025-03-30 | Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain C
1646–1859（214 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6；277 K；0.1M PCB buffer (sodium propionate, sodium cacodylate, BIS-TRIS propane in the molar ratios 2:1:2) pH 6, 25%PEG 1500
|
Resolution 3.00 Å R-free 0.312 |