当前蛋白身份：P43379
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差异标签只比较当前检索结果；所有PDB和assembly原始记录仍分别保留。
相关结构差异明细
一行代表一个 PDB 条目中的一个 biological assembly；同一蛋白的多个单体会分别列出。
| PDB 条目 | Assembly / 聚集状态 | 构建体 | 突变与修饰 | 配体、离子与非聚合物 | 实验方法 | 实验环境 | 结构质量 |
|---|---|---|---|---|---|---|---|
| 1CDG NUCLEOTIDE SEQUENCE AND X-RAY STRUCTURE OF CYCLODEXTRIN GLYCOSYLTRANSFERASE FROM BACILLUS CIRCULANS STRAIN 251 IN A MALTOSE-DEPENDENT CRYSTAL FORM 提交 1993-08-02 | Assembly 1 其他组合 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
28–713（686 aa）
|
未记录 | CA CALCIUM ION × 2 | X-RAY DIFFRACTION | mmCIF 未提供已读取条件 | 分辨率 2.00 Å |
| 1CGV SITE DIRECTED MUTATIONS OF THE ACTIVE SITE RESIDUE TYROSINE 195 OF CYCLODEXTRIN GLYCOSYLTRANSFERASE FROM BACILLUS CIRCULANS STRAIN 251 AFFECTING ACTIVITY AND PRODUCT SPECIFICITY 提交 1994-08-05 | Assembly 1 其他组合 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
28–713（686 aa）
|
突变：Y195F | CA CALCIUM ION × 2 | X-RAY DIFFRACTION | mmCIF 未提供已读取条件 | 分辨率 2.50 Å |
| 1CGW SITE DIRECTED MUTATIONS OF THE ACTIVE SITE RESIDUE TYROSINE 195 OF CYCLODEXTRIN GLYCOSYLTRANSFERASE FROM BACILLUS CIRCULANS STRAIN 251 AFFECTING ACTIVITY AND PRODUCT SPECIFICITY 提交 1994-08-05 | Assembly 1 其他组合 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
28–713（686 aa）
|
突变：Y195G | CA CALCIUM ION × 2 | X-RAY DIFFRACTION | mmCIF 未提供已读取条件 | 分辨率 2.50 Å |
| 1CGX SITE DIRECTED MUTATIONS OF THE ACTIVE SITE RESIDUE TYROSINE 195 OF CYCLODEXTRIN GLYXOSYLTRANSFERASE FROM BACILLUS CIRCULANS STRAIN 251 AFFECTING ACTIVITY AND PRODUCT SPECIFICITY 提交 1994-08-05 | Assembly 1 其他组合 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
28–713（686 aa）
|
未记录 | CA CALCIUM ION × 2 | X-RAY DIFFRACTION | mmCIF 未提供已读取条件 | 分辨率 2.50 Å |
| 1CGY SITE DIRECTED MUTATIONS OF THE ACTIVE SITE RESIDUE TYROSINE 195 OF CYCLODEXTRIN GLYXOSYLTRANSFERASE FROM BACILLUS CIRCULANS STRAIN 251 AFFECTING ACTIVITY AND PRODUCT SPECIFICITY 提交 1994-08-05 | Assembly 1 其他组合 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
28–713（686 aa）
|
未记录 | CA CALCIUM ION × 2 | X-RAY DIFFRACTION | mmCIF 未提供已读取条件 | 分辨率 2.50 Å |
| 1CXE COMPLEX OF CGTASE WITH MALTOTETRAOSE AT ROOM TEMPERATURE AND PH 9.1 BASED ON DIFFRACTION DATA OF A CRYSTAL SOAKED WITH ALPHA-CYCLODEXTRIN 提交 1995-07-28 | Assembly 1 其他组合 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
28–713（686 aa）
|
未记录 | CA CALCIUM ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 9.1；pH 9.1
|
分辨率 2.10 Å R-free 0.100 |
| 1CXF COMPLEX OF A (D229N/E257Q) DOUBLE MUTANT CGTASE FROM BACILLUS CIRCULANS STRAIN 251 WITH MALTOTETRAOSE AT 120 K AND PH 9.1 OBTAINED AFTER SOAKING THE CRYSTAL WITH ALPHA-CYCLODEXTRIN 提交 1995-07-28 | Assembly 1 其他组合 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
28–713（686 aa）
|
突变：D229N, E257Q | CA CALCIUM ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 9.1；pH 9.1
|
分辨率 2.10 Å R-free 0.200 |
| 1CXH COMPLEX OF CGTASE WITH MALTOTETRAOSE AT ROOM TEMPERATURE AND PH 9.6 BASED ON DIFFRACTION DATA OF A CRYSTAL SOAKED WITH MALTOHEPTAOSE 提交 1995-07-31 | Assembly 1 其他组合 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
28–713（686 aa）
|
未记录 | CA CALCIUM ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 9.6；pH 9.6
|
分辨率 2.41 Å R-free 0.200 |
| 1CXI WILD-TYPE CGTASE FROM BACILLUS CIRCULANS STRAIN 251 AT 120 K AND PH 7.55 提交 1995-07-31 | Assembly 1 其他组合 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
28–713（686 aa）
|
未记录 | CA CALCIUM ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 7.55；pH 7.55
|
分辨率 2.20 Å R-free 0.200 |
| 1CXK COMPLEX BETWEEN A MALTONONAOSE SUBSTRATE AND BACILLUS CIRCULANS STRAIN 251 CGTASE E257Q/D229N 提交 1999-02-24 | Assembly 1 其他组合 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
28–713（686 aa）
|
突变：E257Q, D229N | CA CALCIUM ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 10.3；pH 10.30
|
分辨率 2.09 Å R-free 0.210 |
| 1D3C MICHAELIS COMPLEX OF BACILLUS CIRCULANS STRAIN 251 CYCLODEXTRIN GLYCOSYLTRANSFERASE WITH GAMMA-CYCLODEXTRIN 提交 1999-09-29 | Assembly 1 其他组合 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
28–713（686 aa）
|
突变：D229N, E257Q | CA CALCIUM ION × 3 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 10.3；298 K；60 % MPD, 100 MM TRIS PH 8.1, pH 10.3, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
分辨率 1.78 Å R-free 0.258 |
| 1DTU BACILLUS CIRCULANS STRAIN 251 CYCLODEXTRIN GLYCOSYLTRANSFERASE: A MUTANT Y89D/S146P COMPLEXED TO AN HEXASACCHARIDE INHIBITOR 提交 2000-01-13 | Assembly 1 其他组合 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
28–713（686 aa）
|
突变：Y89D, S146P | CA CALCIUM ION × 2 ADH 1-AMINO-2,3-DIHYDROXY-5-HYDROXYMETHYL CYCLOHEX-5-ENE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.1；298 K；60 % MPD,
100 MM TRIS PH 7.1,
5% (w/v) maltose, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
分辨率 2.40 Å R-free 0.248 |
| 1EO5 Bacillus circulans strain 251 cyclodextrin glycosyltransferase in complex with maltoheptaose 提交 2000-03-22 | Assembly 1 其他组合 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
28–713（686 aa）
|
突变：E257A,D229A | CA CALCIUM ION × 3 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 6.1；60% MPD, 100 mM Tris, 5% maltose, pH 6.10, VAPOR DIFFUSION, HANGING DROP
|
分辨率 2.00 Å R-free 0.210 |
| 1EO7 BACILLUS CIRCULANS STRAIN 251 CYCLODEXTRIN GLYCOSYLTRANSFERASE IN COMPLEX WITH MALTOHEXAOSE 提交 2000-03-22 | Assembly 1 其他组合 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
28–713（686 aa）
|
突变：E257A/D229A | CA CALCIUM ION × 3 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 6.1；293 K；60% MPD, 100 mM Tris,
5% w/v maltose, pH 6.1, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
分辨率 2.48 Å R-free 0.296 |
| 1KCK Bacillus circulans strain 251 Cyclodextrin glycosyl transferase mutant N193G 提交 2001-11-09 | Assembly 1 其他组合 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
28–713（686 aa）
|
突变：N193G | ADH 1-AMINO-2,3-DIHYDROXY-5-HYDROXYMETHYL CYCLOHEX-5-ENE × 1 GLC alpha-D-glucopyranose × 1 CA CALCIUM ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；295 K；MPD. Ca, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
分辨率 2.43 Å R-free 0.205 |
| 1OT1 Bacillus circulans strain 251 Cyclodextrin glycosyl transferase mutant D135A 提交 2003-03-21 | Assembly 1 其他组合 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
28–713（686 aa）
|
突变：D135A | CA CALCIUM ION × 3 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 ACY ACETIC ACID × 4 EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；295 K；MPD, Ca, HEPES, maltose, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
分辨率 2.00 Å R-free 0.181 |
| 1OT2 Bacillus circulans strain 251 Cyclodextrin glycosyl transferase mutant D135N 提交 2003-03-21 | Assembly 1 其他组合 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
28–713（686 aa）
|
突变：D135N | CA CALCIUM ION × 3 GLC alpha-D-glucopyranose × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 ACY ACETIC ACID × 3 EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；295 K；MPD, Ca, HEPES, maltose, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
分辨率 2.10 Å R-free 0.184 |
| 1PEZ Bacillus circulans strain 251 mutant A230V 提交 2003-05-23 | Assembly 1 其他组合 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
28–713（686 aa）
|
突变：A230V | CA CALCIUM ION × 3 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 1 ACY ACETIC ACID × 8 EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；295 K；MPD, Ca, HEPES, maltose, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
分辨率 2.32 Å R-free 0.199 |
| 1PJ9 Bacillus circulans strain 251 loop mutant 183-195 提交 2003-06-02 | Assembly 1 其他组合 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
28–713（686 aa）
|
突变：T185S, T186Y, N188D, K192R | GLC alpha-D-glucopyranose × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 1 ACY ACETIC ACID × 3 CA CALCIUM ION × 3 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；295 K；MPD, Ca, HEPES, maltose, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
分辨率 2.00 Å R-free 0.176 |
| 1TCM CYCLODEXTRIN GLYCOSYLTRANSFERASE W616A MUTANT FROM BACILLUS CIRCULANS STRAIN 251 提交 1996-10-07 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
28–713（686 aa）
|
突变：W616A | CA CALCIUM ION × 2 | X-RAY DIFFRACTION | mmCIF 未提供已读取条件 | 分辨率 2.20 Å R-free 0.250 |
| 1TCM CYCLODEXTRIN GLYCOSYLTRANSFERASE W616A MUTANT FROM BACILLUS CIRCULANS STRAIN 251 提交 1996-10-07 | Assembly 2 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 B
28–713（686 aa）
|
突变：W616A | CA CALCIUM ION × 2 | X-RAY DIFFRACTION | mmCIF 未提供已读取条件 | 分辨率 2.20 Å R-free 0.250 |
| 2CXG CYCLODEXTRIN GLYCOSYLTRANSFERASE COMPLEXED TO THE INHIBITOR ACARBOSE 提交 1998-05-08 | Assembly 1 其他组合 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
28–713（686 aa）
|
未记录 | ACI 6-AMINO-4-HYDROXYMETHYL-CYCLOHEX-4-ENE-1,2,3-TRIOL × 1 GLC alpha-D-glucopyranose × 1 CA CALCIUM ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 7.55；PROTEIN WAS CRYSTALLIZED FROM OTHER LIQUOR CONTAINING 60% V/V METHYL-PENTANEDIOL (MPD) 100 MM SODIUM-HEPES PH 7.55 AND 0.5% W/V MALTOSE THEN SOAKED IN MOTHER LIQUOR WITH 0.1 % ACARBOSE AND 0.5 % MALTOSE FOR 5 DAYS
|
分辨率 2.50 Å R-free 0.230 |
| 2DIJ COMPLEX OF A Y195F MUTANT CGTASE FROM B. CIRCULANS STRAIN 251 COMPLEXED WITH A MALTONONAOSE INHIBITOR AT PH 9.8 OBTAINED AFTER SOAKING THE CRYSTAL WITH ACARBOSE AND MALTOHEXAOSE 提交 1998-05-27 | Assembly 1 其他组合 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
28–713（686 aa）
|
突变：Y195F | CA CALCIUM ION × 2 ADH 1-AMINO-2,3-DIHYDROXY-5-HYDROXYMETHYL CYCLOHEX-5-ENE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 9.8；SOAKED WITH ACARBOSE AND MALTOHEXAOSE, pH 9.8
|
分辨率 2.60 Å |