当前蛋白身份：P62483
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差异标签只比较当前检索结果；所有PDB和assembly原始记录仍分别保留。
相关结构差异明细
一行代表一个 PDB 条目中的一个 biological assembly；同一蛋白的多个单体会分别列出。
| PDB 条目 | Assembly / 聚集状态 | 构建体 | 突变与修饰 | 配体、离子与非聚合物 | 实验方法 | 实验环境 | 结构质量 |
|---|---|---|---|---|---|---|---|
| 1EXB STRUCTURE OF THE CYTOPLASMIC BETA SUBUNIT-T1 ASSEMBLY OF VOLTAGE-DEPENDENT K CHANNELS 提交 2000-05-02 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 8 PDB 声明：octameric（8） 与蛋白数一致 |
链 A
36–367（332 aa）
片段：BETA SUBUNIT, RESIDUES 36-367
|
未记录 | NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 4 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8.6；277 K；Peg 4000, glycine, pH 8.6, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
分辨率 2.10 Å R-free 0.227 |
| 1QRQ STRUCTURE OF A VOLTAGE-DEPENDENT K+ CHANNEL BETA SUBUNIT 提交 1999-06-15 | Assembly 1 蛋白同源多聚体 同源多聚体；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 A
36–360（325 aa）
片段：BETA SUBUNIT CORE
链 B
36–360（325 aa）
片段：BETA SUBUNIT CORE
链 C
36–360（325 aa）
片段：BETA SUBUNIT CORE
链 D
36–360（325 aa）
片段：BETA SUBUNIT CORE
|
未记录 | NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 4 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 7.5；pH 7.50
|
分辨率 2.80 Å R-free 0.242 |
| 1QRQ STRUCTURE OF A VOLTAGE-DEPENDENT K+ CHANNEL BETA SUBUNIT 提交 1999-06-15 | Assembly 2 蛋白同源多聚体 同源多聚体；蛋白 × 8 PDB 声明：octameric（8） 与蛋白数一致 |
链 A
36–360（325 aa）
片段：BETA SUBUNIT CORE
链 B
36–360（325 aa）
片段：BETA SUBUNIT CORE
链 C
36–360（325 aa）
片段：BETA SUBUNIT CORE
链 D
36–360（325 aa）
片段：BETA SUBUNIT CORE
|
未记录 | NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 8 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 7.5；pH 7.50
|
分辨率 2.80 Å R-free 0.242 |
| 2A79 Mammalian Shaker Kv1.2 potassium channel- beta subunit complex 提交 2005-07-05 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 16 PDB 声明：hexadecameric（16） 与蛋白数一致 |
链 A
36–367（332 aa）
|
未记录 | NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 4 K POTASSIUM ION × 24 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 8.5；293 K；peg 400, potassium chloride, tris buffer, EDTA, DTT, TCEP, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
分辨率 2.90 Å R-free 0.252 |
| 2R9R Shaker family voltage dependent potassium channel (kv1.2-kv2.1 paddle chimera channel) in association with beta subunit 提交 2007-09-13 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 8 PDB 声明：octameric（8） 与蛋白数一致 |
链 A
36–367（332 aa）
|
未记录 | NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 4 K POTASSIUM ION × 16 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 64 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 8.5；293 K；peg 400, potassium chloride, tris buffer, EDTA, DTT, TCEP, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K
|
分辨率 2.40 Å R-free 0.244 |
| 2R9R Shaker family voltage dependent potassium channel (kv1.2-kv2.1 paddle chimera channel) in association with beta subunit 提交 2007-09-13 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 8 PDB 声明：octameric（8） 与蛋白数一致 |
链 G
36–367（332 aa）
|
未记录 | NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 4 K POTASSIUM ION × 16 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 4 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 8.5；293 K；peg 400, potassium chloride, tris buffer, EDTA, DTT, TCEP, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K
|
分辨率 2.40 Å R-free 0.244 |
| 2R9R Shaker family voltage dependent potassium channel (kv1.2-kv2.1 paddle chimera channel) in association with beta subunit 提交 2007-09-13 | Assembly 3 蛋白异源复合物 异源复合物；蛋白 × 16 PDB 声明：hexadecameric（16） 与蛋白数一致 |
链 A
36–367（332 aa）
链 G
36–367（332 aa）
|
未记录 | NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 8 K POTASSIUM ION × 32 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 68 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 8.5；293 K；peg 400, potassium chloride, tris buffer, EDTA, DTT, TCEP, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K
|
分辨率 2.40 Å R-free 0.244 |
| 2R9R Shaker family voltage dependent potassium channel (kv1.2-kv2.1 paddle chimera channel) in association with beta subunit 提交 2007-09-13 | Assembly 4 蛋白同源多聚体 同源多聚体；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 A
36–367（332 aa）
|
未记录 | NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 4 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 8.5；293 K；peg 400, potassium chloride, tris buffer, EDTA, DTT, TCEP, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K
|
分辨率 2.40 Å R-free 0.244 |
| 2R9R Shaker family voltage dependent potassium channel (kv1.2-kv2.1 paddle chimera channel) in association with beta subunit 提交 2007-09-13 | Assembly 6 蛋白同源多聚体 同源多聚体；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 G
36–367（332 aa）
|
未记录 | NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 4 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 8.5；293 K；peg 400, potassium chloride, tris buffer, EDTA, DTT, TCEP, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K
|
分辨率 2.40 Å R-free 0.244 |
| 3EAU Voltage-dependent K+ channel beta subunit in complex with cortisone 提交 2008-08-26 | Assembly 1 蛋白同源多聚体 同源多聚体；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 A
36–361（326 aa）
片段：cytoplasmic Kvbeta subunit
|
未记录 | NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 4 PDN 17,21-DIHYDROXYPREGNA-1,4-DIENE-3,11,20-TRIONE × 8 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；293 K；6-15% glycerol, 1.5 M ammonium sulfate, 0.1 M Tris, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
分辨率 1.82 Å R-free 0.206 |
| 3EB3 Voltage-dependent K+ channel beta subunit (W121A) in complex with cortisone 提交 2008-08-26 | Assembly 1 蛋白同源多聚体 同源多聚体；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 A
36–361（326 aa）
片段：cytoplasmic Kvbeta subunit
|
突变：W121A | NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 4 PDN 17,21-DIHYDROXYPREGNA-1,4-DIENE-3,11,20-TRIONE × 4 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；293 K；6-15% glycerol, 1.5 M ammonium sulfate, 0.1 M Tris, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
分辨率 2.00 Å R-free 0.249 |
| 3EB4 Voltage-dependent K+ channel beta subunit (I211R) in complex with cortisone 提交 2008-08-26 | Assembly 1 蛋白同源多聚体 同源多聚体；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 A
36–361（326 aa）
片段：cytoplasmic Kvbeta subunit
|
突变：I211R | NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 4 PDN 17,21-DIHYDROXYPREGNA-1,4-DIENE-3,11,20-TRIONE × 4 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；293 K；6-15% glycerol, 1.5 M ammonium sulfate, 0.1 M Tris, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
分辨率 2.00 Å R-free 0.256 |
| 3LNM F233W mutant of the Kv2.1 paddle-Kv1.2 chimera channel 提交 2010-02-02 | Assembly 1 信息不足 异源复合物；蛋白 × 8 PDB 声明：octameric（8） 与蛋白数一致 |
链 A
36–367（332 aa）
片段：subunit beta-2 core
|
未记录 | NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 4 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 4 K POTASSIUM ION × 20 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；293 K；25-28% PEG 400, 50mM Tris-HCl, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
分辨率 2.90 Å R-free 0.247 |
| 3LNM F233W mutant of the Kv2.1 paddle-Kv1.2 chimera channel 提交 2010-02-02 | Assembly 2 信息不足 异源复合物；蛋白 × 8 PDB 声明：octameric（8） 与蛋白数一致 |
链 C
36–367（332 aa）
片段：subunit beta-2 core
|
未记录 | NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 4 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 48 K POTASSIUM ION × 20 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；293 K；25-28% PEG 400, 50mM Tris-HCl, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
分辨率 2.90 Å R-free 0.247 |
| 3LUT A Structural Model for the Full-length Shaker Potassium Channel Kv1.2 提交 2010-02-18 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 8 PDB 声明：octameric（8） 与蛋白数一致 |
链 A
1–367（367 aa）
|
未记录 | NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 4 K POTASSIUM ION × 24 | X-RAY DIFFRACTION | mmCIF 未提供已读取条件 | 分辨率 2.90 Å R-free 0.221 |
| 4JTA Crystal structure of Kv1.2-2.1 paddle chimera channel in complex with Charybdotoxin 提交 2013-03-23 | Assembly 1 信息不足 异源复合物；蛋白 × 8 PDB 声明：octameric（8） 与蛋白数一致 |
链 A
36–367（332 aa）
|
未记录 | NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 4 K POTASSIUM ION × 12 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 64 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 8.9；293 K；PEG 400, POTASSIUM CHLORIDE, TRIS , pH 8.9, vapor diffusion, hanging drop, temperature 293K
|
分辨率 2.50 Å R-free 0.236 |
| 4JTA Crystal structure of Kv1.2-2.1 paddle chimera channel in complex with Charybdotoxin 提交 2013-03-23 | Assembly 2 信息不足 异源复合物；蛋白 × 9 PDB 声明：nonameric（9） 与蛋白数一致 |
链 P
36–367（332 aa）
|
未记录 | NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 4 K POTASSIUM ION × 12 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 4 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 8.9；293 K；PEG 400, POTASSIUM CHLORIDE, TRIS , pH 8.9, vapor diffusion, hanging drop, temperature 293K
|
分辨率 2.50 Å R-free 0.236 |
| 4JTC Crystal structure of Kv1.2-2.1 paddle chimera channel in complex with Charybdotoxin in Cs+ 提交 2013-03-23 | Assembly 1 信息不足 异源复合物；蛋白 × 8 PDB 声明：octameric（8） 与蛋白数一致 |
链 A
36–367（332 aa）
|
未记录 | NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 4 CS CESIUM ION × 16 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 64 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 8.9；293 K；PEG 400, Cesium CHLORIDE, TRIS BUFFER, pH 8.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
分辨率 2.56 Å R-free 0.262 |
| 4JTC Crystal structure of Kv1.2-2.1 paddle chimera channel in complex with Charybdotoxin in Cs+ 提交 2013-03-23 | Assembly 2 信息不足 异源复合物；蛋白 × 9 PDB 声明：nonameric（9） 与蛋白数一致 |
链 G
36–367（332 aa）
|
未记录 | NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 4 CS CESIUM ION × 16 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 4 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 8.9；293 K；PEG 400, Cesium CHLORIDE, TRIS BUFFER, pH 8.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
分辨率 2.56 Å R-free 0.262 |
| 4JTD Crystal structure of Kv1.2-2.1 paddle chimera channel in complex with Lys27Met mutant of Charybdotoxin 提交 2013-03-23 | Assembly 1 信息不足 异源复合物；蛋白 × 8 PDB 声明：octameric（8） 与蛋白数一致 |
链 A
36–367（332 aa）
|
未记录 | NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 4 K POTASSIUM ION × 16 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 64 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 8.9；293 K；PEG 400, POTASSIUM CHLORIDE, TRIS , pH 8.9, vapor diffusion, hanging drop, temperature 293K
|
分辨率 2.54 Å R-free 0.235 |
| 4JTD Crystal structure of Kv1.2-2.1 paddle chimera channel in complex with Lys27Met mutant of Charybdotoxin 提交 2013-03-23 | Assembly 2 信息不足 异源复合物；蛋白 × 9 PDB 声明：nonameric（9） 与蛋白数一致 |
链 G
36–367（332 aa）
|
未记录 | NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 4 K POTASSIUM ION × 16 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 4 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 8.9；293 K；PEG 400, POTASSIUM CHLORIDE, TRIS , pH 8.9, vapor diffusion, hanging drop, temperature 293K
|
分辨率 2.54 Å R-free 0.235 |
| 5WIE Crystal structure of a Kv1.2-2.1 chimera K+ channel V406W mutant in an inactivated state 提交 2017-07-19 | Assembly 1 信息不足 异源复合物；蛋白 × 8 PDB 声明：octameric（8） 与蛋白数一致 |
链 A
36–367（332 aa）
|
未记录 | NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 4 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 20 K POTASSIUM ION × 16 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 8；293 K；50 mM Tris-Cl pH 8.3, 29-31% PEG400
|
分辨率 3.30 Å R-free 0.243 |
| 5WIE Crystal structure of a Kv1.2-2.1 chimera K+ channel V406W mutant in an inactivated state 提交 2017-07-19 | Assembly 2 信息不足 异源复合物；蛋白 × 8 PDB 声明：octameric（8） 与蛋白数一致 |
链 G
36–367（332 aa）
|
未记录 | NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 4 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 4 K POTASSIUM ION × 16 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 8；293 K；50 mM Tris-Cl pH 8.3, 29-31% PEG400
|
分辨率 3.30 Å R-free 0.243 |
| 6CI1 The Structure of Full-Length Kv Beta 2.1 Determined by Cryogenic Electron Microscopy 提交 2018-02-23 | Assembly 1 蛋白同源多聚体 同源多聚体；蛋白 × 8 PDB 声明：octameric（8） 与蛋白数一致 |
链 A
36–361（326 aa）
链 B
36–361（326 aa）
链 C
36–361（326 aa）
链 D
36–361（326 aa）
链 E
36–361（326 aa）
链 F
36–361（326 aa）
链 G
36–361（326 aa）
链 H
36–361（326 aa）
|
未记录 | 未记录非水小分子 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 8
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 4.90 Å |
| 6EBK The voltage-activated Kv1.2-2.1 paddle chimera channel in lipid nanodiscs 提交 2018-08-06 | Assembly 1 信息不足 异源复合物；蛋白 × 8 PDB 声明：octameric（8） 与蛋白数一致 |
链 A
37–367（331 aa）
链 C
37–367（331 aa）
链 E
37–367（331 aa）
链 G
37–367（331 aa）
|
突变：cytosolic domain (UNP residues 37-367) 突变：cytosolic domain (UNP residues 37-367) 突变：cytosolic domain (UNP residues 37-367) 突变：cytosolic domain (UNP residues 37-367) | NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 4 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE；A 3 microliter sample was applied to a plasma-cleaned grid and blotted for 10 seconds.
|
分辨率 3.30 Å |
| 6EBL The voltage-activated Kv1.2-2.1 paddle chimera channel in lipid nanodiscs, cytosolic domain 提交 2018-08-06 | Assembly 1 信息不足 异源复合物；蛋白 × 8 PDB 声明：octameric（8） 与蛋白数一致 |
链 A
37–367（331 aa）
链 C
37–367（331 aa）
链 E
37–367（331 aa）
链 G
37–367（331 aa）
|
突变：cytosolic domain (UNP residues 37-367) 突变：cytosolic domain (UNP residues 37-367) 突变：cytosolic domain (UNP residues 37-367) 突变：cytosolic domain (UNP residues 37-367) | NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 4 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE；A 3 microliter sample was applied to a plasma-cleaned grid and blotted for 10 seconds.
|
分辨率 3.00 Å |
| 7SIT Crystal structure of Voltage gated potassium ion channel, Kv 1.2 chimera-3m 提交 2021-10-14 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 8 PDB 声明：octameric（8） 与蛋白数一致 |
链 A
36–367（332 aa）
|
未记录 | NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 4 K POTASSIUM ION × 8 O OXYGEN ATOM × 4 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 8.5；293 K；50 mM Tris, pH 8.5, 28-32% PEG400, CHAPS
|
分辨率 3.32 Å R-free 0.289 |
| 7SIT Crystal structure of Voltage gated potassium ion channel, Kv 1.2 chimera-3m 提交 2021-10-14 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 8 PDB 声明：octameric（8） 与蛋白数一致 |
链 C
36–367（332 aa）
|
未记录 | NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 4 K POTASSIUM ION × 8 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 8.5；293 K；50 mM Tris, pH 8.5, 28-32% PEG400, CHAPS
|
分辨率 3.32 Å R-free 0.289 |
| 7SIZ C-type inactivation in a voltage gated K+ channel 提交 2021-10-15 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 8 PDB 声明：octameric（8） 与蛋白数一致 |
链 A
36–367（332 aa）
|
未记录 | NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 4 K POTASSIUM ION × 24 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 8.6；293 K；50 mM Tris, pH 8.6, 28-30% PEG400, 8 mM CHAPS
|
分辨率 3.10 Å R-free 0.265 |
| 7SIZ C-type inactivation in a voltage gated K+ channel 提交 2021-10-15 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 8 PDB 声明：octameric（8） 与蛋白数一致 |
链 C
36–367（332 aa）
|
未记录 | NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 4 K POTASSIUM ION × 24 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 8.6；293 K；50 mM Tris, pH 8.6, 28-30% PEG400, 8 mM CHAPS
|
分辨率 3.10 Å R-free 0.265 |