当前蛋白身份:P85421 重新检索
本组结构的主要差异维度
构建体不同 组装状态不同 配体/离子不同 实验方法不同 实验环境不同 结构质量指标不同

差异标签只比较当前检索结果;所有PDB和assembly原始记录仍分别保留。

相关结构差异明细

一行代表一个 PDB 条目中的一个 biological assembly;同一蛋白的多个单体会分别列出。

PDB 条目 Assembly / 聚集状态 构建体 突变与修饰 配体、离子与非聚合物 实验方法 实验环境 结构质量
1K83 Crystal Structure of Yeast RNA Polymerase II Complexed with the Inhibitor Alpha Amanitin 提交 2001-10-22 Assembly 1 蛋白异源复合物 异源复合物;蛋白 × 11 PDB 声明:undecameric(11) 与蛋白数一致
链 M 1–8(8 aa)
非标准单体:是(mmCIF未提供具体位点) ZN ZINC ION × 8 MN MANGANESE (II) ION × 1 X-RAY DIFFRACTION
X-ray结晶条件 pH 6;PH 6.00
分辨率 2.80 Å R-free 0.280
2VUM Alpha-amanitin inhibited complete RNA polymerase II elongation complex 提交 2008-05-27 Assembly 1 其他组合 异源复合物;蛋白 × 13 PDB 声明:hexadecameric(16) 与全部聚合物一致
链 M 7–8(2 aa)
链 M 1–6(6 aa)
非标准单体:是(mmCIF未提供具体位点) 非标准单体:是(mmCIF未提供具体位点) ZN ZINC ION × 8 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray结晶条件 pH 7;200 MM AMMONIUM ACETATE, 300 MM SODIUM ACETATE, 50 MM HEPES PH 7.0, 4-7% PEG 6000, 5 MM TCEP
分辨率 3.40 Å R-free 0.288
3CQZ Crystal structure of 10 subunit RNA polymerase II in complex with the inhibitor alpha-amanitin 提交 2008-04-03 Assembly 1 蛋白异源复合物 异源复合物;蛋白 × 11 PDB 声明:undecameric(11) 与蛋白数一致
链 M 1–8(8 aa)
非标准单体:是(mmCIF未提供具体位点) ZN ZINC ION × 8 X-RAY DIFFRACTION mmCIF 未提供已读取条件 分辨率 2.80 Å R-free 0.273
6EXV Structure of mammalian RNA polymerase II elongation complex inhibited by Alpha-amanitin 提交 2017-11-09 Assembly 1 其他组合 异源复合物;蛋白 × 13 PDB 声明:hexadecameric(16) 与全部聚合物一致
链 M 1–8(8 aa)
非标准单体:是(mmCIF未提供具体位点) ZN ZINC ION × 8 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM缓冲液 pH 7.6
cryo-EM玻璃化条件 冷冻剂 ETHANE
分辨率 3.60 Å
8WAK Structure of transcribing complex 2 (TC2), the initially transcribing complex with Pol II positioned 2nt downstream of TSS. 提交 2023-09-07 Assembly 1 其他组合 异源复合物;蛋白 × 48 PDB 声明:51-meric(51) 与全部聚合物一致
链 N 1–8(8 aa)
非标准单体:是(mmCIF未提供具体位点) ZN ZINC ION × 17 SF4 IRON/SULFUR CLUSTER × 1 W0F [[(2~{S},3~{R},4~{S},5~{R})-5-(2-azanyl-6-oxidanylidene-3~{H}-purin-9-yl)-3-methoxy-4-oxidanyl-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] phosphono hydrogen phosphate × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM缓冲液 pH 7.9
cryo-EM玻璃化条件 冷冻剂 ETHANE
分辨率 5.47 Å
8WAL Structure of transcribing complex 3 (TC3), the initially transcribing complex with Pol II positioned 3nt downstream of TSS. 提交 2023-09-07 Assembly 1 其他组合 异源复合物;蛋白 × 48 PDB 声明:51-meric(51) 与全部聚合物一致
链 N 1–8(8 aa)
非标准单体:是(mmCIF未提供具体位点) ZN ZINC ION × 17 SF4 IRON/SULFUR CLUSTER × 1 W0F [[(2~{S},3~{R},4~{S},5~{R})-5-(2-azanyl-6-oxidanylidene-3~{H}-purin-9-yl)-3-methoxy-4-oxidanyl-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] phosphono hydrogen phosphate × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM缓冲液 pH 7.9
cryo-EM玻璃化条件 冷冻剂 ETHANE
分辨率 8.52 Å
8WAN Structure of transcribing complex 4 (TC4), the initially transcribing complex with Pol II positioned 4nt downstream of TSS. 提交 2023-09-07 Assembly 1 其他组合 异源复合物;蛋白 × 48 PDB 声明:51-meric(51) 与全部聚合物一致
链 N 1–8(8 aa)
非标准单体:是(mmCIF未提供具体位点) ZN ZINC ION × 17 SF4 IRON/SULFUR CLUSTER × 1 MG MAGNESIUM ION × 1 W0F [[(2~{S},3~{R},4~{S},5~{R})-5-(2-azanyl-6-oxidanylidene-3~{H}-purin-9-yl)-3-methoxy-4-oxidanyl-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] phosphono hydrogen phosphate × 1 ELECTRON MICROSCOPY
cryo-EM缓冲液 pH 7.9
cryo-EM玻璃化条件 冷冻剂 ETHANE
分辨率 6.07 Å
8WAO Structure of transcribing complex 5 (TC5), the initially transcribing complex with Pol II positioned 5nt downstream of TSS. 提交 2023-09-07 Assembly 1 其他组合 异源复合物;蛋白 × 48 PDB 声明:51-meric(51) 与全部聚合物一致
链 N 1–8(8 aa)
非标准单体:是(mmCIF未提供具体位点) ZN ZINC ION × 17 SF4 IRON/SULFUR CLUSTER × 1 MG MAGNESIUM ION × 1 W0F [[(2~{S},3~{R},4~{S},5~{R})-5-(2-azanyl-6-oxidanylidene-3~{H}-purin-9-yl)-3-methoxy-4-oxidanyl-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] phosphono hydrogen phosphate × 1 ELECTRON MICROSCOPY
cryo-EM缓冲液 pH 7.9
cryo-EM玻璃化条件 冷冻剂 ETHANE
分辨率 6.40 Å
8WAP Structure of transcribing complex 6 (TC6), the initially transcribing complex with Pol II positioned 6nt downstream of TSS. 提交 2023-09-08 Assembly 1 其他组合 异源复合物;蛋白 × 48 PDB 声明:51-meric(51) 与全部聚合物一致
链 N 1–8(8 aa)
非标准单体:是(mmCIF未提供具体位点) ZN ZINC ION × 17 SF4 IRON/SULFUR CLUSTER × 1 MG MAGNESIUM ION × 1 W0F [[(2~{S},3~{R},4~{S},5~{R})-5-(2-azanyl-6-oxidanylidene-3~{H}-purin-9-yl)-3-methoxy-4-oxidanyl-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] phosphono hydrogen phosphate × 1 ELECTRON MICROSCOPY
cryo-EM缓冲液 pH 7.9
cryo-EM玻璃化条件 冷冻剂 ETHANE
分辨率 5.85 Å
8WAQ Structure of transcribing complex 7 (TC7), the initially transcribing complex with Pol II positioned 7nt downstream of TSS. 提交 2023-09-08 Assembly 1 其他组合 异源复合物;蛋白 × 48 PDB 声明:51-meric(51) 与全部聚合物一致
链 N 1–8(8 aa)
非标准单体:是(mmCIF未提供具体位点) ZN ZINC ION × 17 SF4 IRON/SULFUR CLUSTER × 1 MG MAGNESIUM ION × 1 W0F [[(2~{S},3~{R},4~{S},5~{R})-5-(2-azanyl-6-oxidanylidene-3~{H}-purin-9-yl)-3-methoxy-4-oxidanyl-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] phosphono hydrogen phosphate × 1 ELECTRON MICROSCOPY
cryo-EM缓冲液 pH 7.9
cryo-EM玻璃化条件 冷冻剂 ETHANE
分辨率 6.29 Å
8WAR Structure of transcribing complex 8 (TC8), the initially transcribing complex with Pol II positioned 8nt downstream of TSS. 提交 2023-09-08 Assembly 1 其他组合 异源复合物;蛋白 × 48 PDB 声明:51-meric(51) 与全部聚合物一致
链 N 1–8(8 aa)
非标准单体:是(mmCIF未提供具体位点) ZN ZINC ION × 17 SF4 IRON/SULFUR CLUSTER × 1 MG MAGNESIUM ION × 1 W0F [[(2~{S},3~{R},4~{S},5~{R})-5-(2-azanyl-6-oxidanylidene-3~{H}-purin-9-yl)-3-methoxy-4-oxidanyl-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] phosphono hydrogen phosphate × 1 ELECTRON MICROSCOPY
cryo-EM缓冲液 pH 7.9
cryo-EM玻璃化条件 冷冻剂 ETHANE
分辨率 7.20 Å
8WAS Structure of transcribing complex 9 (TC9), the initially transcribing complex with Pol II positioned 9nt downstream of TSS. 提交 2023-09-08 Assembly 1 其他组合 异源复合物;蛋白 × 48 PDB 声明:51-meric(51) 与全部聚合物一致
链 N 1–8(8 aa)
非标准单体:是(mmCIF未提供具体位点) ZN ZINC ION × 17 SF4 IRON/SULFUR CLUSTER × 1 MG MAGNESIUM ION × 1 W0F [[(2~{S},3~{R},4~{S},5~{R})-5-(2-azanyl-6-oxidanylidene-3~{H}-purin-9-yl)-3-methoxy-4-oxidanyl-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] phosphono hydrogen phosphate × 1 ELECTRON MICROSCOPY
cryo-EM缓冲液 pH 7.9
cryo-EM玻璃化条件 冷冻剂 ETHANE
分辨率 6.13 Å
8WAT De novo transcribing complex 10 (TC10), the early elongation complex with Pol II positioned 10nt downstream of TSS 提交 2023-09-08 Assembly 1 其他组合 异源复合物;蛋白 × 15 PDB 声明:octadecameric(18) 与全部聚合物一致
链 N 1–8(8 aa)
非标准单体:是(mmCIF未提供具体位点) ZN ZINC ION × 8 MG MAGNESIUM ION × 1 W0F [[(2~{S},3~{R},4~{S},5~{R})-5-(2-azanyl-6-oxidanylidene-3~{H}-purin-9-yl)-3-methoxy-4-oxidanyl-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] phosphono hydrogen phosphate × 1 ELECTRON MICROSCOPY
cryo-EM缓冲液 pH 7.9
cryo-EM玻璃化条件 冷冻剂 ETHANE
分辨率 2.82 Å
8WAU De novo transcribing complex 11 (TC11), the early elongation complex with Pol II positioned 11nt downstream of TSS 提交 2023-09-08 Assembly 1 其他组合 异源复合物;蛋白 × 15 PDB 声明:octadecameric(18) 与全部聚合物一致
链 N 1–8(8 aa)
非标准单体:是(mmCIF未提供具体位点) W0F [[(2~{S},3~{R},4~{S},5~{R})-5-(2-azanyl-6-oxidanylidene-3~{H}-purin-9-yl)-3-methoxy-4-oxidanyl-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] phosphono hydrogen phosphate × 1 ZN ZINC ION × 8 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM缓冲液 pH 7.9
cryo-EM玻璃化条件 冷冻剂 ETHANE
分辨率 2.78 Å
8WAV De novo transcribing complex 12 (TC12), the early elongation complex with Pol II positioned 12nt downstream of TSS 提交 2023-09-08 Assembly 1 其他组合 异源复合物;蛋白 × 15 PDB 声明:octadecameric(18) 与全部聚合物一致
链 N 1–8(8 aa)
非标准单体:是(mmCIF未提供具体位点) ZN ZINC ION × 8 MG MAGNESIUM ION × 1 W0F [[(2~{S},3~{R},4~{S},5~{R})-5-(2-azanyl-6-oxidanylidene-3~{H}-purin-9-yl)-3-methoxy-4-oxidanyl-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] phosphono hydrogen phosphate × 1 ELECTRON MICROSCOPY
cryo-EM缓冲液 pH 7.9
cryo-EM玻璃化条件 冷冻剂 ETHANE
分辨率 2.72 Å
8WAW De novo transcribing complex 13 (TC13), the early elongation complex with Pol II positioned 13nt downstream of TSS 提交 2023-09-08 Assembly 1 其他组合 异源复合物;蛋白 × 15 PDB 声明:octadecameric(18) 与全部聚合物一致
链 N 1–8(8 aa)
非标准单体:是(mmCIF未提供具体位点) ZN ZINC ION × 8 MG MAGNESIUM ION × 1 W0F [[(2~{S},3~{R},4~{S},5~{R})-5-(2-azanyl-6-oxidanylidene-3~{H}-purin-9-yl)-3-methoxy-4-oxidanyl-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] phosphono hydrogen phosphate × 1 ELECTRON MICROSCOPY
cryo-EM缓冲液 pH 7.9
cryo-EM玻璃化条件 冷冻剂 ETHANE
分辨率 3.02 Å
8WAX De novo transcribing complex 14 (TC14), the early elongation complex with Pol II positioned 14nt downstream of TSS 提交 2023-09-08 Assembly 1 其他组合 异源复合物;蛋白 × 15 PDB 声明:octadecameric(18) 与全部聚合物一致
链 N 1–8(8 aa)
非标准单体:是(mmCIF未提供具体位点) ZN ZINC ION × 8 MG MAGNESIUM ION × 1 W0F [[(2~{S},3~{R},4~{S},5~{R})-5-(2-azanyl-6-oxidanylidene-3~{H}-purin-9-yl)-3-methoxy-4-oxidanyl-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] phosphono hydrogen phosphate × 1 ELECTRON MICROSCOPY
cryo-EM缓冲液 pH 7.9
cryo-EM玻璃化条件 冷冻剂 ETHANE
分辨率 2.75 Å
8WAY De novo transcribing complex 15 (TC15), the early elongation complex with Pol II positioned 15nt downstream of TSS 提交 2023-09-08 Assembly 1 其他组合 异源复合物;蛋白 × 15 PDB 声明:octadecameric(18) 与全部聚合物一致
链 N 1–8(8 aa)
非标准单体:是(mmCIF未提供具体位点) ZN ZINC ION × 8 MG MAGNESIUM ION × 1 W0F [[(2~{S},3~{R},4~{S},5~{R})-5-(2-azanyl-6-oxidanylidene-3~{H}-purin-9-yl)-3-methoxy-4-oxidanyl-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] phosphono hydrogen phosphate × 1 ELECTRON MICROSCOPY
cryo-EM缓冲液 pH 7.9
cryo-EM玻璃化条件 冷冻剂 ETHANE
分辨率 2.85 Å
8WAZ De novo transcribing complex 16 (TC16), the early elongation complex with Pol II positioned 16nt downstream of TSS 提交 2023-09-08 Assembly 1 其他组合 异源复合物;蛋白 × 15 PDB 声明:octadecameric(18) 与全部聚合物一致
链 N 1–8(8 aa)
非标准单体:是(mmCIF未提供具体位点) ZN ZINC ION × 8 MG MAGNESIUM ION × 1 W0F [[(2~{S},3~{R},4~{S},5~{R})-5-(2-azanyl-6-oxidanylidene-3~{H}-purin-9-yl)-3-methoxy-4-oxidanyl-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] phosphono hydrogen phosphate × 1 ELECTRON MICROSCOPY
cryo-EM缓冲液 pH 7.9
cryo-EM玻璃化条件 冷冻剂 ETHANE
分辨率 2.76 Å
8WB0 De novo transcribing complex 17 (TC17), the early elongation complex with Pol II positioned 17nt downstream of TSS 提交 2023-09-08 Assembly 1 其他组合 异源复合物;蛋白 × 15 PDB 声明:octadecameric(18) 与全部聚合物一致
链 N 1–8(8 aa)
非标准单体:是(mmCIF未提供具体位点) ZN ZINC ION × 8 MG MAGNESIUM ION × 1 W0F [[(2~{S},3~{R},4~{S},5~{R})-5-(2-azanyl-6-oxidanylidene-3~{H}-purin-9-yl)-3-methoxy-4-oxidanyl-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] phosphono hydrogen phosphate × 1 ELECTRON MICROSCOPY
cryo-EM缓冲液 pH 7.9
cryo-EM玻璃化条件 冷冻剂 ETHANE
分辨率 2.94 Å