1k28

The Structure of the Bacteriophage T4 Cell-Puncturing Device

Method: X-RAY DIFFRACTION Dmax: 190.0 Å Quality: REASONABLE

SAXS 散射曲线 SAXS Profile

SAXS profile for 1k28

P(r) 距离分布 P(r) Distribution

P(r) distribution for 1k28
下载 Download

1. 结构基本信息 1. Structure Basics

条目编号 entry_id1k28
沉积日期 deposition_date2001-09-26
结构标题 titleThe Structure of the Bacteriophage T4 Cell-Puncturing Device
关键词 keywordsTriple-stranded beta-helix, OB fold, pseudohexamer, T4 tail lysozyme, HUB, gp27-gp5*-gp5C, HYDROLASE-STRUCTURAL PROTEIN COMPLEX; HYDROLASE/STRUCTURAL PROTEIN
实验方法 methodX-RAY DIFFRACTION

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier48.90
回转半径 Rg (电子) rg_electron50.45
零角强度 I(0) i0170704000.00
分子量 molecular_weight103520.0 kDa
排除体积 excluded_volume127910 ų
包络体积 envelope_volume215890 ų
水化壳体积 shell_volume39492 ų
包络直径 envelope_diameter197.6
壳层 Rg shell_rg45.21
包络 Rg envelope_rg54.33
形状 Rg shape_rg50.44
总 Rg total_rg50.24
总原子数 total_atoms7225
残基数 n_residues906
球谐函数阶数 n_harmonics20
q 范围 q_range— – 0.5000 −1
数据点数 n_points101
壳层类型 shell_typedirectional
溶剂电子密度 solvent_density0.3340 e/ų
壳层衬度 contrast_shell0.0300 e/ų
CRYSOL 版本 crysol_version4.1.3

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax190.0
Rg (实空间) rg_real50.37
Rg 误差 (实空间) rg_real_error3.50
I(0) (实空间) i0_real1.7070e+08
I(0) 误差 (实空间) i0_real_error3.8040e+06
Rg (倒空间) rg_reciprocal48.91
I(0) (倒空间) i0_reciprocal170400000.0000
解质量估计 total_estimate0.6562
解质量评级 solution_quality REASONABLE a REASONABLE solution
P(r) 峰数 n_peaks2
主峰位置 r_peak_primary29.7
偏度 Skewness skewness0.745
峰度 Kurtosis kurtosis0.055
角度范围 angular_range— – 0.1600 −1
当前正则化参数 α current_alpha0.0000
最高正则化参数 α highest_alpha10640000.0000
实空间数据点数 n_real_points33
GNOM 版本 gnom_version4.1.3
质量判据 quality_criteria AN1: 0.000; Oscil: 0.252; Stabil: 0.999; Sysdev: 1.000; Positv: 1.000; Valcen: 0.108; Smooth: 0.666

4. 晶体学与实验 4. Crystallography & Experiment

5. 实体与聚合体信息 (5)

6. 折叠分类 (SCOP + CATH) 13 domains

SCOP 2.08 (6 domains)

结构域编号 domain_idd1k28a1
类 Class classb — All beta proteins
折叠类型 Fold foldb.40 — OB-fold
超家族 Superfamily superfamilyb.40.8 — gp5 N-terminal domain-like
家族 Family familyb.40.8.1 — gp4 N-terminal domain-like
结构域编号 domain_idd1k28a2
类 Class classb — All beta proteins
折叠类型 Fold foldb.108 — Triple-stranded beta-helix
超家族 Superfamily superfamilyb.108.1 — Phage fibre proteins
家族 Family familyb.108.1.2 — Tail-associated lysozyme gp5, C-terminal domain
结构域编号 domain_idd1k28a3
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.2 — Lysozyme-like
超家族 Superfamily superfamilyd.2.1 — Lysozyme-like
家族 Family familyd.2.1.3 — Phage lysozyme
结构域编号 domain_idd1k28a4
类 Class classl — Artifacts
折叠类型 Fold foldl.1 — Tags
超家族 Superfamily superfamilyl.1.1 — Tags
家族 Family familyl.1.1.1 — Tags
结构域编号 domain_idd1k28d1
类 Class classb — All beta proteins
折叠类型 Fold foldb.106 — Phage tail proteins
超家族 Superfamily superfamilyb.106.1 — Phage tail proteins
家族 Family familyb.106.1.1 — Baseplate protein-like
结构域编号 domain_idd1k28d2
类 Class classb — All beta proteins
折叠类型 Fold foldb.106 — Phage tail proteins
超家族 Superfamily superfamilyb.106.1 — Phage tail proteins
家族 Family familyb.106.1.1 — Baseplate protein-like

CATH v4.4 (7 domains)

结构域编号 domain_id1k28A01
类 Class class2 — Mainly Beta
架构 Architecture architecture40 — Beta Barrel
拓扑 Topology topology50 — OB fold (Dihydrolipoamide Acetyltransferase, E2P)
同源超家族 H-superfamily homologous superfamily260 — Nucleic acid-binding protein domain
结构域编号 domain_id1k28A02
类 Class class3 — Alpha Beta
架构 Architecture architecture10 — Roll
拓扑 Topology topology450 — Nuclear Transport Factor 2; Chain: A,
同源超家族 H-superfamily homologous superfamily190
结构域编号 domain_id1k28A03
类 Class class1 — Mainly Alpha
架构 Architecture architecture10 — Orthogonal Bundle
拓扑 Topology topology530 — Lysozyme
同源超家族 H-superfamily homologous superfamily40
结构域编号 domain_id1k28D01
类 Class class2 — Mainly Beta
架构 Architecture architecture40 — Beta Barrel
拓扑 Topology topology10 — Thrombin, subunit H
同源超家族 H-superfamily homologous superfamily10 — Trypsin-like serine proteases
结构域编号 domain_id1k28D02
类 Class class3 — Alpha Beta
架构 Architecture architecture55 — 3-Layer(bab) Sandwich
拓扑 Topology topology50 — Phage tail protein beta-alpha-beta fold
同源超家族 H-superfamily homologous superfamily20
结构域编号 domain_id1k28D03
类 Class class2 — Mainly Beta
架构 Architecture architecture40 — Beta Barrel
拓扑 Topology topology30 — Elongation Factor Tu (Ef-tu); domain 3
同源超家族 H-superfamily homologous superfamily150 — Bacteriophage T4, Gp27, baseplate hub, domain 3
结构域编号 domain_id1k28D04
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology1920 — Phage tail proteins - 2 layer sandwich fold
同源超家族 H-superfamily homologous superfamily40

7. 引用文献 (1)

8. 文件与曲线 (10)