4iqz

The crystal structure of a large insert in RNA polymerase (RpoC) subunit from E. coli

Method: X-RAY DIFFRACTION Dmax: 106.7 Å Quality: REASONABLE

1. 蛋白身份与相关结构 Protein Identity & Related Structures

;DNA-directed RNA polymerase subunit beta' ;

Escherichia coli

UniProt P0A8T7

当前结构中的状态

Assembly 聚集状态 构建体 突变与修饰 配体、离子与共同组分 实验方法与环境 结构质量
1 蛋白单体 单体 蛋白 × 1 PDB 声明:monomeric(1) 与蛋白拷贝数一致 链 A; UniProt 932–1141 片段:unp residues 932-1141 非标准单体:是(mmCIF未提供具体位点) IOD IODIDE ION × 15 NA SODIUM ION × 2 X-RAY DIFFRACTION X-ray结晶条件:VAPOR DIFFUSION, HANGING DROP;pH 4.2;277 K;0.2 M KCl, 0.04 M Tris pH 7.9, 0.1 M Phosphate-Citrate, 1.6 M Sodium di-Hydrogen Phosphate, 0.4 M di-Potassium Hydrogen Phosphate, 0.05 M Sodium Iodide , VAPOR DIFFUSION, HANGING DROP, temperature 277K 分辨率 2.10 Å R-free 0.240
2 蛋白单体 单体 蛋白 × 1 PDB 声明:monomeric(1) 与蛋白拷贝数一致 链 B; UniProt 932–1141 片段:unp residues 932-1141 非标准单体:是(mmCIF未提供具体位点) IOD IODIDE ION × 13 NA SODIUM ION × 1 X-RAY DIFFRACTION X-ray结晶条件:VAPOR DIFFUSION, HANGING DROP;pH 4.2;277 K;0.2 M KCl, 0.04 M Tris pH 7.9, 0.1 M Phosphate-Citrate, 1.6 M Sodium di-Hydrogen Phosphate, 0.4 M di-Potassium Hydrogen Phosphate, 0.05 M Sodium Iodide , VAPOR DIFFUSION, HANGING DROP, temperature 277K 分辨率 2.10 Å R-free 0.240
3 蛋白单体 单体 蛋白 × 1 PDB 声明:monomeric(1) 与蛋白拷贝数一致 链 C; UniProt 932–1141 片段:unp residues 932-1141 非标准单体:是(mmCIF未提供具体位点) IOD IODIDE ION × 11 NA SODIUM ION × 2 X-RAY DIFFRACTION X-ray结晶条件:VAPOR DIFFUSION, HANGING DROP;pH 4.2;277 K;0.2 M KCl, 0.04 M Tris pH 7.9, 0.1 M Phosphate-Citrate, 1.6 M Sodium di-Hydrogen Phosphate, 0.4 M di-Potassium Hydrogen Phosphate, 0.05 M Sodium Iodide , VAPOR DIFFUSION, HANGING DROP, temperature 277K 分辨率 2.10 Å R-free 0.240
4 蛋白单体 单体 蛋白 × 1 PDB 声明:monomeric(1) 与蛋白拷贝数一致 链 D; UniProt 932–1141 片段:unp residues 932-1141 非标准单体:是(mmCIF未提供具体位点) IOD IODIDE ION × 13 NA SODIUM ION × 1 X-RAY DIFFRACTION X-ray结晶条件:VAPOR DIFFUSION, HANGING DROP;pH 4.2;277 K;0.2 M KCl, 0.04 M Tris pH 7.9, 0.1 M Phosphate-Citrate, 1.6 M Sodium di-Hydrogen Phosphate, 0.4 M di-Potassium Hydrogen Phosphate, 0.05 M Sodium Iodide , VAPOR DIFFUSION, HANGING DROP, temperature 277K 分辨率 2.10 Å R-free 0.240
5 蛋白单体 单体 蛋白 × 1 PDB 声明:monomeric(1) 与蛋白拷贝数一致 链 E; UniProt 932–1141 片段:unp residues 932-1141 非标准单体:是(mmCIF未提供具体位点) IOD IODIDE ION × 4 NA SODIUM ION × 1 X-RAY DIFFRACTION X-ray结晶条件:VAPOR DIFFUSION, HANGING DROP;pH 4.2;277 K;0.2 M KCl, 0.04 M Tris pH 7.9, 0.1 M Phosphate-Citrate, 1.6 M Sodium di-Hydrogen Phosphate, 0.4 M di-Potassium Hydrogen Phosphate, 0.05 M Sodium Iodide , VAPOR DIFFUSION, HANGING DROP, temperature 277K 分辨率 2.10 Å R-free 0.240

数据库中的同蛋白其他状态

以下每一行都是同一 UniProt 蛋白在另一个 PDB 条目中的 biological assembly, “相对当前条目”直接指出证据层面的不同;没有差异标签表示当前已读取字段一致。

共 239 个其他 PDB 条目、271 个 assembly。 打开独立比较页并筛选聚集状态

查看构建体与数据证据
UniProt名称 RPOC_ECOLI
Isoform
PDB实体 1
链与序列区间 作者链 A; PDB构建体 21–230; UniProt 932–1141 作者链 B; PDB构建体 21–230; UniProt 932–1141 作者链 C; PDB构建体 21–230; UniProt 932–1141 作者链 D; PDB构建体 21–230; UniProt 932–1141 作者链 E; PDB构建体 21–230; UniProt 932–1141

页面优先展示蛋白身份、当前 assembly、共同组分、聚集状态和跨 PDB 结构链接。 链映射与序列区间收在“数据证据”中;数据库内部编号、导入时间和 assembly 操作表达式仅用于维护,因此不在读者页面展示。

SAXS 散射曲线 SAXS Profile

SAXS profile for 4iqz

P(r) 距离分布 P(r) Distribution

P(r) distribution for 4iqz
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2. 结构基本信息 2. Structure Basics

条目编号 entry_id4iqz
沉积日期 deposition_date2013-01-14
结构标题 titleThe crystal structure of a large insert in RNA polymerase (RpoC) subunit from E. coli
关键词 keywordsFunction of insertion is unknown, UNKNOWN FUNCTION; UNKNOWN FUNCTION
实验方法 methodX-RAY DIFFRACTION

3. SAXS 参数 (CRYSOL 理论计算) 3. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier32.30
回转半径 Rg (电子) rg_electron32.05
零角强度 I(0) i0169441000.00
分子量 molecular_weight91280.0 kDa
排除体积 excluded_volume106770 ų
包络体积 envelope_volume143230 ų
水化壳体积 shell_volume38211 ų
包络直径 envelope_diameter115.3
壳层 Rg shell_rg37.90
包络 Rg envelope_rg32.07
形状 Rg shape_rg32.21
总 Rg total_rg32.03
总原子数 total_atoms5935
残基数 n_residues836
球谐函数阶数 n_harmonics20
q 范围 q_range— – 0.5000 −1
数据点数 n_points101
壳层类型 shell_typedirectional
溶剂电子密度 solvent_density0.3340 e/ų
壳层衬度 contrast_shell0.0300 e/ų
CRYSOL 版本 crysol_version4.1.3

4. P(r) 距离分布 (GNOM 反演) 4. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax106.7
Rg (实空间) rg_real32.28
Rg 误差 (实空间) rg_real_error0.66
I(0) (实空间) i0_real1.6940e+08
I(0) 误差 (实空间) i0_real_error2.6350e+06
Rg (倒空间) rg_reciprocal32.29
I(0) (倒空间) i0_reciprocal169400000.0000
解质量估计 total_estimate0.6892
解质量评级 solution_quality REASONABLE a REASONABLE solution
P(r) 峰数 n_peaks2
主峰位置 r_peak_primary34.8
偏度 Skewness skewness0.281
峰度 Kurtosis kurtosis-0.498
角度范围 angular_range— – 0.2450 −1
当前正则化参数 α current_alpha0.0000
最高正则化参数 α highest_alpha26760000.0000
实空间数据点数 n_real_points50
GNOM 版本 gnom_version4.1.3
质量判据 quality_criteria AN1: 0.000; Oscil: 0.900; Stabil: 1.000; Sysdev: 0.096; Positv: 1.000; Valcen: 0.989; Smooth: 0.978

5. 晶体学与实验 5. Crystallography & Experiment

6. 实体与聚合物信息 Entities & Polymers (4)

7. 折叠分类 (SCOP + CATH) 10 domains

CATH v4.4 (10 domains)

结构域编号 domain_id4iqzA01
类 Class class2 — Mainly Beta
架构 Architecture architecture40 — Beta Barrel
拓扑 Topology topology50 — OB fold (Dihydrolipoamide Acetyltransferase, E2P)
同源超家族 H-superfamily homologous superfamily100 — RNA polymerase II/Efflux pump adaptor protein, barrel-sandwich hybrid domain
结构域编号 domain_id4iqzA02
类 Class class2 — Mainly Beta
架构 Architecture architecture40 — Beta Barrel
拓扑 Topology topology50 — OB fold (Dihydrolipoamide Acetyltransferase, E2P)
同源超家族 H-superfamily homologous superfamily100 — RNA polymerase II/Efflux pump adaptor protein, barrel-sandwich hybrid domain
结构域编号 domain_id4iqzB01
类 Class class2 — Mainly Beta
架构 Architecture architecture40 — Beta Barrel
拓扑 Topology topology50 — OB fold (Dihydrolipoamide Acetyltransferase, E2P)
同源超家族 H-superfamily homologous superfamily100 — RNA polymerase II/Efflux pump adaptor protein, barrel-sandwich hybrid domain
结构域编号 domain_id4iqzB02
类 Class class2 — Mainly Beta
架构 Architecture architecture40 — Beta Barrel
拓扑 Topology topology50 — OB fold (Dihydrolipoamide Acetyltransferase, E2P)
同源超家族 H-superfamily homologous superfamily100 — RNA polymerase II/Efflux pump adaptor protein, barrel-sandwich hybrid domain
结构域编号 domain_id4iqzC01
类 Class class2 — Mainly Beta
架构 Architecture architecture40 — Beta Barrel
拓扑 Topology topology50 — OB fold (Dihydrolipoamide Acetyltransferase, E2P)
同源超家族 H-superfamily homologous superfamily100 — RNA polymerase II/Efflux pump adaptor protein, barrel-sandwich hybrid domain
结构域编号 domain_id4iqzC02
类 Class class2 — Mainly Beta
架构 Architecture architecture40 — Beta Barrel
拓扑 Topology topology50 — OB fold (Dihydrolipoamide Acetyltransferase, E2P)
同源超家族 H-superfamily homologous superfamily100 — RNA polymerase II/Efflux pump adaptor protein, barrel-sandwich hybrid domain
结构域编号 domain_id4iqzD01
类 Class class2 — Mainly Beta
架构 Architecture architecture40 — Beta Barrel
拓扑 Topology topology50 — OB fold (Dihydrolipoamide Acetyltransferase, E2P)
同源超家族 H-superfamily homologous superfamily100 — RNA polymerase II/Efflux pump adaptor protein, barrel-sandwich hybrid domain
结构域编号 domain_id4iqzD02
类 Class class2 — Mainly Beta
架构 Architecture architecture40 — Beta Barrel
拓扑 Topology topology50 — OB fold (Dihydrolipoamide Acetyltransferase, E2P)
同源超家族 H-superfamily homologous superfamily100 — RNA polymerase II/Efflux pump adaptor protein, barrel-sandwich hybrid domain
结构域编号 domain_id4iqzE01
类 Class class2 — Mainly Beta
架构 Architecture architecture40 — Beta Barrel
拓扑 Topology topology50 — OB fold (Dihydrolipoamide Acetyltransferase, E2P)
同源超家族 H-superfamily homologous superfamily100 — RNA polymerase II/Efflux pump adaptor protein, barrel-sandwich hybrid domain
结构域编号 domain_id4iqzE02
类 Class class2 — Mainly Beta
架构 Architecture architecture40 — Beta Barrel
拓扑 Topology topology50 — OB fold (Dihydrolipoamide Acetyltransferase, E2P)
同源超家族 H-superfamily homologous superfamily100 — RNA polymerase II/Efflux pump adaptor protein, barrel-sandwich hybrid domain

8. 引用文献 (1)

9. 文件与曲线 (10)