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5u5p

Crystal Structure and X-ray Diffraction Data Collection of Importin-alpha from Mus Musculus Complexed with a MLH1 NLS Peptide

Method: X-RAY DIFFRACTION Dmax: 98.5 Å Quality: REASONABLE
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1. Protein Identity and Related Structures Protein Identity & Related Structures

DNA mismatch repair protein Mlh1

OrganismNot specified

UniProt P40692

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 3 PDB declaration: trimeric（3） Consistent with protein copy count Chain B； UniProt 467–476 Chain C； UniProt 467–476 Not recorded Importin subunit alpha-1 × 1 （P52293） DTT 2,3-DIHYDROXY-1,4-DITHIOBUTANE × 1 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；291 K；0.600-0.625 M sodium citrate (pH 6) 10 mM DTT Resolution 2.17 Å R-free 0.211

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5U5P Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
3RBN Crystal structure of MutL protein homolog 1 isoform 1 [Homo sapiens] Deposited 2011-03-29 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 486–751（266 aa） Fragment：UNP residues 486-751
Chain B 486–751（266 aa） Fragment：UNP residues 486-751
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；22% PEG4000, 0.2 M sodium acetate, 0.1 M Tris-HCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.16 Å R-free 0.251
4P7A Crystal Structure of human MLH1 Deposited 2014-03-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–347（347 aa）
Not recorded MG MAGNESIUM ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 UNX UNKNOWN LIGAND × 18 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；20% PEG4000, 10% ISOPROPANOL, 0.1 M HEPES
Resolution 2.30 Å R-free 0.254
6WBA Structure of Mouse Importin alpha MLH1- R470A NLS Peptide Complex Deposited 2020-03-26 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 466–476（11 aa） Fragment：NLS peptide
Chain C 466–476（11 aa） Fragment：NLS peptide
Mutation：R470A Mutation：R470A No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；291 K；0.1 M sodium citrate (pH 6), 0.6 M sodium citrate and 10 mM DTT
Resolution 2.15 Å R-free 0.197
6WBB Structure of Mouse Importin alpha - MLH1-E475A NLS peptide complex Deposited 2020-03-26 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 466–476（11 aa） Fragment：NLS peptide
Chain C 466–476（11 aa） Fragment：NLS peptide
Mutation：E475A Mutation：E475A No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；291 K；0.1 M sodium citrate (pH 6), 10 mM DTT, 0.55 M sodium citrate
Resolution 2.66 Å R-free 0.227
6WBC Structure of Mouse Importin alpha- MLH1-R472K NLS Peptide Complex Deposited 2020-03-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 466–476（11 aa） Fragment：NLS peptide
Mutation：R472K No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；291 K；0.1 M sodium citrate (pH 6), 0.575 M sodium citrate , 10 mM DTT
Resolution 2.15 Å R-free 0.213
7M60 Structure of Mouse Importin alpha MLH1-S467A NLS Peptide Complex Deposited 2021-03-25 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 466–476（11 aa） Fragment：residues 466-476
Chain C 466–476（11 aa） Fragment：residues 466-476
Mutation：S467A Mutation：S467A No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；291 K；0.1M sodium citrate pH6, 0.65 M sodium citrate, 10 mM DTT
Resolution 2.30 Å R-free 0.207

6 other PDB entries and 6 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name MLH1_HUMAN
Isoform —
PDB entities 1
Chains and sequence ranges Author chain B； PDBConstruct 1–10； UniProt 467–476 Author chain C； PDBConstruct 1–10； UniProt 467–476

Importin subunit alpha-1

Mus musculus

UniProt P52293

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 3 PDB declaration: trimeric（3） Consistent with protein copy count Chain A； UniProt 70–529 Fragment：residues 70-529 DNA mismatch repair protein Mlh1 × 2 （P40692） DTT 2,3-DIHYDROXY-1,4-DITHIOBUTANE × 1 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；291 K；0.600-0.625 M sodium citrate (pH 6) 10 mM DTT Resolution 2.17 Å R-free 0.211

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5U5P Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
1EJL MOUSE IMPORTIN ALPHA-SV40 LARGE T ANTIGEN NLS PEPTIDE COMPLEX Deposited 2000-03-03 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain I 70–529（460 aa） Fragment：NLS-BINDING DOMAIN, RESIDUES 70-529
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；sodium citrate, Hepes, DTT, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.80 Å R-free 0.254
1EJY MOUSE IMPORTIN ALPHA-NUCLEOPLASMIN NLS PEPTIDE COMPLEX Deposited 2000-03-05 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain I 70–529（460 aa） Fragment：NLS BINDING DOMAIN, RESIDUES 70-529
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；sodium citrate, Hepes, DTT, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.90 Å R-free 0.268
1IAL IMPORTIN ALPHA, MOUSE Deposited 1999-01-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 44–496（453 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 6；pH 6.0
Resolution 2.50 Å R-free 0.240
1IQ1 CRYSTAL STRUCTURE OF THE IMPORTIN-ALPHA(44-54)-IMPORTIN-ALPHA(70-529) COMPLEX Deposited 2001-05-28 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric
Chain A 44–54（11 aa） Fragment：AUTOINHIBITORY PEPTIDE(RESIDUES 44-54)
Chain B 44–54（11 aa） Fragment：AUTOINHIBITORY PEPTIDE(RESIDUES 44-54)
Chain C 70–529（460 aa） Fragment：ARMADILLO REPEAT DOMAIN(RESIDUES 70-529)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；291 K；sodium phosphate, DTT, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 2.80 Å R-free 0.248
1PJM Mouse Importin alpha-bipartite NLS from human retinoblastoma protein Complex Deposited 2003-06-03 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 70–529（460 aa） Fragment：NLS binding domain (70-529)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；293 K；Sodium Citrate, DTT, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.50 Å R-free 0.239
1PJN Mouse Importin alpha-bipartite NLS N1N2 from Xenopus laevis phosphoprotein Complex Deposited 2003-06-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 531–552（22 aa） Fragment：NLS (nuclear localization signal) bipartite peptide
Mutation：Q555G No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；293 K；Sodium Citrate, DTT, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.50 Å R-free 0.233
1Y2A Structure of mammalian importin bound to the non-classical PLSCR1-NLS Deposited 2004-11-22 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain C 70–497（428 aa） Fragment：residues 70-497
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；298 K；0.6-0.7 M sodium citrate, 100 mM Hepes pH 6.0, 10 mM -mercaptoethonal, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
Resolution 2.20 Å R-free 0.258
23QH Structure of importin alpha bound to the Psittacine Adenovirus B Fiber 1 protein nuclear localization signal Deposited 2026-02-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain E 70–529（460 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；296 K；0.6 M sodium citrate, 0.1 M HEPES
Resolution 2.10 Å R-free 0.215
2C1M Nup50:importin-alpha complex Deposited 2005-09-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 75–498（424 aa） Fragment：DELTA IBB, RESIDUES 75-498
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions DETAILS GIVEN IN PUBLICATION
Resolution 2.20 Å R-free 0.254
3BTR AR-NLS:Importin-alpha complex Deposited 2007-12-30 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain C 70–496（427 aa） Fragment：UNP residues 70-496
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；291 K；pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 2.60 Å R-free 0.229
3KND TPX2:importin-alpha complex Deposited 2009-11-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 70–529（460 aa） Fragment：residues 70-529
Not recorded SO4 SULFATE ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；291 K；1.1-1.2 M ammonium sulphate, 100 mM Tris-Hcl, 10 mM DTT, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 2.15 Å R-free 0.214
3L3Q Mouse importin alpha-pepTM NLS peptide complex Deposited 2009-12-17 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 71–497（427 aa） Fragment：NLS binding domain (UNP residues 71-497)
Not recorded FLC CITRATE ANION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；293 K；Sodium Citrate, DTT, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.30 Å R-free 0.227
3OQS Crystal structure of importin-alpha bound to a CLIC4 NLS peptide Deposited 2010-09-03 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 70–529（460 aa） Fragment：NLS Binding Domain (UNP residues 70-529)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.4；293 K；0.7M sodium citrate, 10mM DTT, 70mM HEPES pH 7.4, vapor diffusion, hanging drop, temperature 293K
Resolution 2.00 Å R-free 0.237
3Q5U A minimal NLS from human scramblase 4 complexed with importin alpha Deposited 2010-12-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 70–529（460 aa） Fragment：unp residues 70-529
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；293 K；2.3 uL of deltaIBB-importin alpha concentrated to 20 mg/ml were mixed with 0.7 microlitre of a 2-fold molar excess of hPLSCR4-NLS peptide; 3 uL of reservoir solution containing ~0.8 M sodium citrate, 100 mM Hepes, and 10 mM beta-mercaptoethanol were added to the drop, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.50 Å R-free 0.226
3RZ9 Mouse importin alpha-Ku80 NLS peptide complex Deposited 2011-05-11 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 70–529（460 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；293 K；Sodium Citrate, DTT, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.29 Å R-free 0.218
3RZX Mouse importin alpha-Ku70 NLS peptide complex Deposited 2011-05-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 70–529（460 aa） Fragment：NLS binding domain
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；293 K；Sodium Citrate, DTT, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.61 Å R-free 0.223
3TPM Crystal structure of MAL RPEL domain in complex with importin-alpha Deposited 2011-09-08 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 75–496（422 aa） Fragment：ARM-repeat domain, UNP residues 75-496
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.4；293 K；50mM Tris-HCl, 1.4M Li2SO4, pH 7.4, VAPOR DIFFUSION, temperature 293K
Resolution 2.10 Å R-free 0.208
3TPO Crystal structure of D192A/E396A mutant of mouse importin alpha2 Deposited 2011-09-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–529（529 aa）
Mutation：D192A, E396A No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；293 K；0.1M HEPES, 1.0M KNa-tartrate, pH 7.0, VAPOR DIFFUSION, temperature 293K
Resolution 2.10 Å R-free 0.192
3UKW Mouse importin alpha: Bimax1 peptide complex Deposited 2011-11-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 70–529（460 aa） Fragment：UNP Residues 70-529
Not recorded BTB 2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；0.8M sodium citrate, 10mM DTT, 0.1M BisTris, 10% glycerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.10 Å R-free 0.200
3UKX Mouse importin alpha: Bimax2 peptide complex Deposited 2011-11-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 70–529（460 aa） Fragment：UNP Residues 70-529
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；0.75M sodium citrate, 10mM DTT, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.20 Å R-free 0.203
3UKY Mouse importin alpha: yeast CBP80 cNLS complex Deposited 2011-11-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 70–529（460 aa） Fragment：UNP Residues 70-529
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；0.8M sodium citrate, 10mM DTT, 0.1M HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.35 Å R-free 0.215
3UKZ Mouse importin alpha: mouse CBP80 cNLS complex Deposited 2011-11-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 70–529（460 aa） Fragment：UNP Residues 70-529
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；0.82M sodium citrate, 10mM DTT, 0.1M HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.30 Å R-free 0.208
3UL0 Mouse importin alpha: mouse CBP80Y8D cNLS complex Deposited 2011-11-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 70–529（460 aa） Fragment：UNP Residues 70-529
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；0.7M sodium citrate, 10mM DTT, 0.1M HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.00 Å R-free 0.208
3UL1 Mouse importin alpha: nucleoplasmin cNLS peptide complex Deposited 2011-11-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 70–529（460 aa） Fragment：UNP Residues 70-529
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.2；293 K；0.79M sodium citrate, 10mM DTT, 0.1M HEPES, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 1.90 Å R-free 0.222
3UVU Structural basis of nuclear import of Flap endonuclease 1 (FEN1) Deposited 2011-11-30 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 70–529（460 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.8；293 K；Sodium citrate, DTT, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.38 Å R-free 0.215
3VE6 Crystal Structure Analysis of Venezuelan Equine Encephalitis Virus Capsid Protein NLS and Importin Alpha Deposited 2012-01-07 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 71–496（426 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；vapor diffusion, hanging drop
Resolution 2.83 Å R-free 0.270
3ZIN Gu_alpha_helicase Deposited 2013-01-10 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 72–496（425 aa） Fragment：RESIDUES 72-496
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.5；0.8 M SODIUM CITRATE, 0.1 M HEPES BUFFER (PH 6.5) AND 10 MM DTT
Resolution 2.00 Å R-free 0.199
3ZIO minor-site specific NLS (A28) Deposited 2013-01-10 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 72–496（425 aa） Fragment：RESIDUES 72-496
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.5；0.65 M SODIUM CITRATE, 0.1 M HEPES BUFFER (PH 6.5) AND 10 MM DTT
Resolution 2.10 Å R-free 0.202
3ZIP minor-site specific NLS (A58) Deposited 2013-01-10 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 72–497（426 aa） Fragment：RESIDUES 72-497
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.5；0.75 M SODIUM CITRATE, 0.1 M HEPES BUFFER (PH 6.5) AND 10 MM DTT
Resolution 2.40 Å R-free 0.191
3ZIQ minor-site specific NLS (B6) Deposited 2013-01-10 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 72–497（426 aa） Fragment：RESIDUES 72-497
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.5；0.7 M SODIUM CITRATE, 0.1 M HEPES BUFFER (PH 6.5) AND 10 MM DTT
Resolution 2.10 Å R-free 0.202
3ZIR minor-site specific NLS (B141) Deposited 2013-01-10 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 72–497（426 aa） Fragment：RESIDUES 72-497
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.5；0.75 M SODIUM CITRATE, 0.1 M HEPES BUFFER (PH 6.5) AND 10 MM DTT
Resolution 2.30 Å R-free 0.202
4BA3 mImp_alphadIBB_A89NLS Deposited 2012-09-11 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 70–529（460 aa） Fragment：NLS BINDING DOMAIN, RESIDUES 70-529
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions 0.8 M SODIUM CITRATE, 0.1 M HEPES BUFFER, PH 6.5 AND 10 MM DTT
Resolution 2.10 Å R-free 0.204
4HTV Mouse importin alpha: BFDV Cap NLS peptide complex Deposited 2012-11-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 70–528（459 aa） Fragment：UNP residues 70-528
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；290 K；0.675 M trisodium citrate, 20 mM DTT, 0.1 M HEPES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K
Resolution 3.00 Å R-free 0.228
4MZ5 Structure of importin-alpha: dUTPase NLS complex Deposited 2013-09-29 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain E 70–528（459 aa） Fragment：UNP residues 70-528
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；Sodium citrate, DTT, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.10 Å R-free 0.232
4MZ6 Structure of importin-alpha: dUTPase S11E NLS mutant complex Deposited 2013-09-29 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain E 70–528（459 aa） Fragment：UNP residues 70-528
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；Sodium citrate, DTT, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 1.88 Å R-free 0.195
4OIH Importin Alpha in Complex with the Bipartite NLS of Prp20 Deposited 2014-01-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 70–529（460 aa） Fragment：UNP residues 70-529
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；296.15 K；500mM sodium citrate, 10mM DTT, pH 6.0-8.0, VAPOR DIFFUSION, HANGING DROP, temperature 296.15K
Resolution 2.10 Å R-free 0.201
4U54 IMPORTIN-ALPHA MINOR NLS SITE INHIBITOR Deposited 2014-07-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: Monomeric
Chain A 74–498（425 aa） Fragment：UNP residues 74-498
Not recorded 3C5 N-methyl-1-[3-(pyridin-3-yl)phenyl]methanamine × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；291 K；See publication for details. Precipitant was 1.05-1.2 M ammonium sulphate, 0.1M citrate pH 6.0, 20-40 mM DTT.
Resolution 2.41 Å R-free 0.227
4U58 IMPORTIN-ALPHA MINOR NLS SITE INHIBITOR Deposited 2014-07-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: Monomeric
Chain A 72–497（426 aa） Fragment：UNP residues 72-497
Not recorded 3C6 N~2~-[4-(pyridin-3-yl)benzoyl]-L-lysinamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；291 K；see publication
Resolution 2.56 Å R-free 0.264
4U5L IMPORTIN-ALPHA MINOR NLS SITE INHIBITOR Deposited 2014-07-25 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: MONOMERIC
Chain A 72–497（426 aa）
Mutation：deleted IBB domain 3D6 N~2~-[4-(pyridin-3-yl)benzyl]-L-lysinamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；291 K；see manuscript
Resolution 2.53 Å R-free 0.222
4U5N IMPORTIN-ALPHA MINOR NLS SITE INHIBITOR Deposited 2014-07-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: MONOMERIC
Chain A 72–497（426 aa）
Not recorded 3D4 N~2~-[4-(pyridin-3-yl)benzyl]-L-lysyl-N-[(1R,2S,3R)-1-{[(2R)-1-amino-1-oxo-3-phenylpropan-2-yl]amino}-1,3-dihydroxybutan-2-yl]glycinamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；291 K；see manuscript
Resolution 2.31 Å R-free 0.221
4U5O IMPORTIN-ALPHA MINOR NLS SITE INHIBITOR Deposited 2014-07-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: MONOMERIC
Chain A 72–497（426 aa）
Not recorded 3D5 N~2~-[4-(pyridin-3-yl)benzyl]-D-lysinamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；291 K；see manuscript
Resolution 2.00 Å R-free 0.247
4U5S IMPORTIN-ALPHA MINOR NLS SITE INHIBITOR Deposited 2014-07-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: Monomeric
Chain A 72–497（426 aa） Fragment：UNP residues 72-497
Not recorded 3D0 N-[(2S)-2-[(N~2~-acetyl-D-lysyl)amino]-3-(pyridin-3-ylmethoxy)propyl]-L-allothreonyl-D-phenylalaninamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；291 K；see manuscript
Resolution 2.12 Å R-free 0.231
4U5U IMPORTIN-ALPHA MINOR NLS SITE INHIBITOR Deposited 2014-07-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: Monomeric
Chain A 72–497（426 aa） Fragment：UNP residues 72-497
Not recorded 3D2 N~2~-[3-(pyridin-3-yl)benzyl]-L-lysinamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；291 K；see manuscript
Resolution 1.96 Å R-free 0.215
4U5V IMPORTIN-ALPHA MINOR NLS SITE INHIBITOR Deposited 2014-07-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: Monomeric
Chain A 72–497（426 aa） Fragment：UNP residues 72-497
Not recorded RH2 N~2~-{[5-(pyridin-3-yl)thiophen-2-yl]methyl}-L-lysinamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；291 K；see manuscript
Resolution 1.97 Å R-free 0.259
4UAF Importin alpha 1 delta IBB in complex with Influenza PB2 nuclear localization domain Deposited 2014-08-08 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 69–529（461 aa）
Not recorded PO4 PHOSPHATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；298 K；0.49 M Na phosphate monobasic 0.91 M K phosphate dibasic pH 6.9
Resolution 1.70 Å R-free 0.193
4YI0 Structure of mouse importin a1 bound to Pom121NLS Deposited 2015-02-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain C 70–529（460 aa） Fragment：unp residues 70-529
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.6；293 K；0.1 M sodium citrate tribasic dihydrate, 0.7 M sodium citrate tribasic dihydrate, 25 mM DTT
Resolution 1.81 Å R-free 0.210
4ZDU Crystal structure of importin-alpha bound to a non-classical nuclear localization signal of the influenza A virus nucleoprotein Deposited 2015-04-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 72–498（427 aa） Fragment：UNP residues 72-498
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；PEG 4000
Resolution 2.30 Å R-free 0.246
5B56 Crystal structure of HIV-1 VPR C-Terminal domain and DIBB-M-Importin-Alpha2 complex Deposited 2016-04-25 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 70–529（460 aa） Fragment：UNP RESIDUES 70-529
Chain B 70–529（460 aa） Fragment：UNP RESIDUES 70-529
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.2；293 K；50MM MES, 100MM AMMONIUM SULFATE, 10MM MGCL2, 20-23%(W/V) PEG8000, 5MM DTT
Resolution 2.30 Å R-free 0.226
5CTT Crystal structure of human SART3/TIP110 NLS-mouse importin alpha complex Deposited 2015-07-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 72–497（426 aa） Fragment：UNP residues 72-497
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；100mM HEPES, pH 6.6, 1.6M sodium citrate, 10mM DTT
Resolution 1.70 Å R-free 0.219
5D5K Crystal Structure NLS from human PARP-2 complexed with Importin alpha delta IBB Deposited 2015-08-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain C 70–529（460 aa） Fragment：unp residues 70-529
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 5.6；295 K；0.6-0.7M sodium citrate, 0.1M sodium citrate buffer pH 5.6, and 7-10mM DTT
Resolution 1.90 Å R-free 0.171
5E6Q Importin alpha binding to XRCC1 NLS peptide Deposited 2015-10-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 70–529（460 aa）
Not recorded SO4 SULFATE ION × 1 GOL GLYCEROL × 1 CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；1.5M Ammonium sulfate, 0.1M bis-tris propane
Resolution 2.31 Å R-free 0.208
5EKF Crystallization and X-ray Diffraction Data Collection of Importin-alpha from Mus musculus Complexed with a XPG NLS Peptide, fragment 1 Deposited 2015-11-03 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 70–529（460 aa） Fragment：UNP residues 70-529
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；291 K；0.6-0.7 M sodium citrate, 10 mM DTT
Resolution 2.00 Å R-free 0.192
5EKG Crystallization and X-ray Diffraction Data Collection of Importin-alpha from Mus musculus Complexed with a XPG NLS Peptide, fragment 2 Deposited 2015-11-03 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 70–529（460 aa） Fragment：UNP residues 70-529
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；291 K；0.6-0.7 M sodium citrate, 10 mM DTT
Resolution 2.80 Å R-free 0.228
5FC8 Mouse importin alpha: Dengue 3 NS5 C-terminal NLS peptide complex Deposited 2015-12-15 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain E 71–529（459 aa） Fragment：UNP residues 71-529
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；1 M sodium citrate, 10 mM DTT
Resolution 2.10 Å R-free 0.207
5GXW Importin and NuMA complex Deposited 2016-09-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 70–497（428 aa） Fragment：UNP residues 70-497
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；1M KCl, 0.8M ammonium sulfate, 100mM Hepes, pH 7.0
Resolution 2.39 Å R-free 0.218
5HHG Mouse importin alpha: Dengue 2 NS5 C-terminal NLS peptide complex Deposited 2016-01-11 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain E 71–497（427 aa） Fragment：UNP residues 71-497
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；1.5M Ammonium Sulfate, 0.1M Sodium HEPES
Resolution 2.20 Å R-free 0.214
5HUW Structure of HSV-1 Large Terminase NLS bound to importin alpha Deposited 2016-01-27 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain C 2–529（528 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；298 K；0.6M sodium citrate, 100 mM Hepes, pH 6.0, and 10 mM beta-mercaptoethanol
Resolution 1.95 Å R-free 0.195
5HUY Structure of HCMV Small Terminase NLS bound to importin alpha Deposited 2016-01-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain C 2–529（528 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；293 K；2.3 uL of deltaIBB-importin alpha concentrated to 20 mg/ml were mixed with 0.7 microlitre of a 2-fold molar excess of HCMV-NLS peptide; 3 uL of reservoir solution containing ~0.7 M sodium citrate, 100 mM Hepes, and 10 mM beta-mercaptoethanol were added to the drop, pH 6.0
Resolution 1.98 Å R-free 0.196
5K9S Importin-alpha in complex with HNF1-beta peptide Deposited 2016-06-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 72–529（458 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；291.15 K；10% PEG 8K, 0.09 M NPS, 20% ethylene glycol, 0.1 M Buffer 1 (Morpheus I)
Resolution 2.40 Å R-free 0.221
5KLR Prototypical P4[R]cNLS Deposited 2016-06-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 70–529（460 aa） Fragment：UNP residues 70-529
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；10mM DTT, 1M sodium citrate pH7.0
Resolution 2.20 Å R-free 0.185
5KLT Prototypical P4[M]cNLS Deposited 2016-06-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 70–529（460 aa） Fragment：UNp residues 70-529
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；296 K；1M Sodium citrate pH6, 10mM DTT
Resolution 2.60 Å R-free 0.228
5SVZ HIV-1 Tat NLS in complex with importin alpha Deposited 2016-08-08 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 70–529（460 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；296 K；1.25 M sodium citrate pH 7 and 10 mM DTT
Resolution 2.00 Å R-free 0.192
5U5R Crystal Structure and X-ray Diffraction Data Collection of Importin-alpha from Mus musculus Complexed with a PMS2 NLS Peptide Deposited 2016-12-07 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 70–529（460 aa）
Not recorded DTT 2,3-DIHYDROXY-1,4-DITHIOBUTANE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；291 K；0.600-0.625 M sodium citrate (pH 6) and 10 mM DTT
Resolution 2.10 Å R-free 0.207
5UMZ Structure of TNRC6A NLS in complex with importin-alpha Deposited 2017-01-30 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 70–528（459 aa） Fragment：UNP residues 70-528
Not recorded GOL GLYCEROL × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；sodium citrate, Hepes 7.0, 10mM DTT
Resolution 1.90 Å R-free 0.182
5V5O Structure of NLS2K of influenza A virus nucleoprotein bound to importin alpha Deposited 2017-03-14 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain C 2–529（528 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；291 K；0.1 M HEPES pH 6.0, 0.6 M sodium citrate tribasic dihydrate, 10 mM b-mercaptoethanol
Resolution 2.24 Å R-free 0.206
5V5P Structure of NLS2R of influenza A virus nucleoprotein bound to importin alpha Deposited 2017-03-14 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain C 2–529（528 aa） Fragment：residues 2-529
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；291 K；0.6 M sodium citrate, 100 mM Hepes, and 10 mM beta-mercaptoethanol
Resolution 2.15 Å R-free 0.210
5W41 Zika MR766 NLS in complex with Importin alpha subunit-1 Deposited 2017-06-08 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 70–529（460 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；1M Sodium citrate, 0.01M DTT, pH6.5
Resolution 2.20 Å R-free 0.203
5W4E Importin binding to Tdt NLS peptide Deposited 2017-06-10 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 71–529（459 aa）
Not recorded GOL GLYCEROL × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；0.1 M HEPES 0.7 M Sodium Citrate
Resolution 2.18 Å R-free 0.192
5W4F Importin binding to pol Mu NLS peptide Deposited 2017-06-10 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 70–529（460 aa）
Not recorded GOL GLYCEROL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；0.1 M HEPES 0.8 M Sodium Citrate
Resolution 1.98 Å R-free 0.199
5W4G Importin binding to NLS peptide of DNA polymerase lambda Deposited 2017-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 70–529（460 aa）
Not recorded SO4 SULFATE ION × 4 GOL GLYCEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；0.1 M Bis Tris Propane 1.4 M Ammonium Sulfate
Resolution 2.04 Å R-free 0.200
5WUM Crystal structure of mouse importin-alpha1 bound to S385-phosphorylated NLS of EBNA1 Deposited 2016-12-19 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 70–529（460 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；MES, sodium citrate, DTT
Resolution 2.00 Å R-free 0.211
5WUN Crystal structure of mouse importin-alpha1 bound to non-phosphorylated NLS of EBNA1 Deposited 2016-12-19 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 70–529（460 aa） Fragment：UNP RESIDUES 70-529
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；MES, sodium citrate, DTT
Resolution 2.20 Å R-free 0.204
5X8N Crystal structure of mouse importin-alpha1 bound to the nuclear localization signal of Epstein-Barr virus EBNA-LP protein Deposited 2017-03-03 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 70–529（460 aa） Fragment：UNP residues 70-529
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；MES, sodium citrate, DTT
Resolution 2.15 Å R-free 0.210
6BVT Importin alpha 1 in cargo free state Deposited 2017-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain E 70–529（460 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；0.01M DTT, 0.1M sodium HEPES, 1M Ammonium Sulfate
Resolution 2.50 Å R-free 0.213
6BW0 Nipah virus W protein C-terminus in complex with Importin alpha 1 Deposited 2017-12-14 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain E 70–529（460 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；0.01M DTT, 0.1M sodium HEPES pH7, 0.7M sodium citrate
Resolution 2.10 Å R-free 0.203
6BW1 Hendra virus W protein C-terminus in complex with Importin alpha 1 Deposited 2017-12-14 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain E 70–529（460 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；0.01M DTT, 0.1M sodium HEPES pH7, 0.7M sodium citrate
Resolution 2.20 Å R-free 0.212
6D7M Crystal structure of the W184R/W231R Importin alpha mutant Deposited 2018-04-25 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 70–529（460 aa）
Mutation：W184R, W231R EDO 1,2-ETHANEDIOL × 3 CL CHLORIDE ION × 1 SIN SUCCINIC ACID × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；0.1M Bis-Tris-Propane, 1.0M Sodium Succinate
Resolution 2.19 Å R-free 0.222
6D7N Crystal structure of the W357R/W399R Importin alpha mutant Deposited 2018-04-25 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain B 70–529（460 aa）
Mutation：W357R, W399R EDO 1,2-ETHANEDIOL × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；1.0M triammonium citrate, 0.1M Bis-Tris-Propane
Resolution 2.30 Å R-free 0.217
6IU7 Crystal structure of importin-alpha1 bound to the 53BP1 nuclear localization signal (wild-type) Deposited 2018-11-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 72–498（427 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.7；293 K；sodium citrate, HEPES, DTT
Resolution 1.90 Å R-free 0.185
6IUA Crystal structure of importin-alpha1 bound to the 53BP1 nuclear localization signal (S1678D) Deposited 2018-11-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 72–498（427 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.7；293 K；sodium citrate, HEPES, DTT
Resolution 1.70 Å R-free 0.179
6IW8 Crystal structure of Importin-alpha and wild-type GM130 Deposited 2018-12-04 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain C 70–498（429 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions EVAPORATION；293 K；0.1M Sodium malonate, 12% w/v Polyethylene glycol 3350 (pH 7.0)
Resolution 2.80 Å R-free 0.255
6IWA Crystal structure of Importin-alpha and phosphoserine GM130 Deposited 2018-12-05 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain C 70–498（429 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions EVAPORATION；293 K；0.1M Sodium citrate tribasic dihydrate, 16% w/v Polyethylene glycol 8000 (pH 5.5)
Resolution 2.40 Å R-free 0.216
6K06 Crystal structure of Importin-alpha and phosphomimetic GM130 Deposited 2019-05-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain C 70–498（429 aa）
Mutation：S25D No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions EVAPORATION；293 K；0.2M Potassium thiocyante, 20% w/v Polyethylene glycol 3350
Resolution 1.75 Å R-free 0.199
6MJL Crystal structure of ChREBP NLS peptide bound to importin alpha. Deposited 2018-09-21 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 72–497（426 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions EVAPORATION；293 K；500 mM Na Citrate 10 mM DTT 20 mM HEPES pH 6.5
Resolution 2.50 Å R-free 0.244
6P6A Structure of Mouse Importin alpha - NIT2 NLS peptide complex Deposited 2019-06-03 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 70–529（460 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；Sodium Citrate, DTT, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.15 Å R-free 0.196
6P6E Structure of Mouse Importin alpha - PAC3 NLS peptide complex Deposited 2019-06-03 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 70–529（460 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；Sodium Citrate, DTT, ph 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.99 Å R-free 0.197
6WBA Structure of Mouse Importin alpha MLH1- R470A NLS Peptide Complex Deposited 2020-03-26 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 70–529（460 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；291 K；0.1 M sodium citrate (pH 6), 0.6 M sodium citrate and 10 mM DTT
Resolution 2.15 Å R-free 0.197
6WBB Structure of Mouse Importin alpha - MLH1-E475A NLS peptide complex Deposited 2020-03-26 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain I 70–529（460 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；291 K；0.1 M sodium citrate (pH 6), 10 mM DTT, 0.55 M sodium citrate
Resolution 2.66 Å R-free 0.227
6WBC Structure of Mouse Importin alpha- MLH1-R472K NLS Peptide Complex Deposited 2020-03-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 70–529（460 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；291 K；0.1 M sodium citrate (pH 6), 0.575 M sodium citrate , 10 mM DTT
Resolution 2.15 Å R-free 0.213
6WX7 SOX2 bound to Importin-alpha 2 Deposited 2020-05-09 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 70–529（460 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；298 K；750mM sodium citrate (pH7), 10 mM DTT
Resolution 2.70 Å R-free 0.227
7JJM Crystal structure of Importin alpha 2 in complex with LSD1 NLS Deposited 2020-07-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 62–529（468 aa）
Not recorded CL CHLORIDE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；295 K；0.7 M sodium citrate, 0.01M DTT, 0.1M sodium HEPES pH 7
Resolution 2.06 Å R-free 0.259
7JK7 Crystal structure of Importin alpha 2 in complex with LSD1 NLS S111E mutant Deposited 2020-07-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 62–529（468 aa）
Not recorded GOL GLYCEROL × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；295 K；0.7 M sodium citrate, 0.01M DTT, 0.1M sodium HEPES pH 7
Resolution 1.96 Å R-free 0.206
7JVO Importin alpha bound to the C-terminus of ACE2 Deposited 2020-08-22 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 70–529（460 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；296 K；600 mM citrate, pH 7.0
Resolution 2.20 Å R-free 0.210
7L04 Crystal Structure of Adeno-Associated Virus Porcine Origin capsid protein in complex with Importin-alpha 2 Deposited 2020-12-11 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain E 70–529（460 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；0.6M Na citrate, 0.01M DTT, 0.1M HEPES pH 6.5
Resolution 2.26 Å R-free 0.234
7LEQ Structure of importin a2 bound to p50 NLS Deposited 2021-01-14 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain E 70–497（428 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；0.6M sodium citrate, 100 Hepes pH 6.0
Resolution 2.24 Å R-free 0.219
7LET Structure of importin a2 bound to the p50- and p65-NLSs Deposited 2021-01-15 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain E 70–497（428 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；298 K；0.6 Na Citrate, 100 mM HEPES pH 6
Resolution 2.40 Å R-free 0.241
7LEU Structure of importin a2 bound to p65-NLS Deposited 2021-01-15 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain E 70–497（428 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；298 K；0.55M Na citrate, 100mM HEPES pH 6.0
Resolution 2.82 Å R-free 0.236
7M60 Structure of Mouse Importin alpha MLH1-S467A NLS Peptide Complex Deposited 2021-03-25 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 70–529（460 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；291 K；0.1M sodium citrate pH6, 0.65 M sodium citrate, 10 mM DTT
Resolution 2.30 Å R-free 0.207
7RFX Importin alpha2 in complex with MERS ORF4B Deposited 2021-07-14 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 70–529（460 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；296 K；0.7M sodium citrate (pH 7.0) and 10 mM DTT
Resolution 2.10 Å R-free 0.208
7RFZ Importin alpha 2 in complex with MERS ORF4B NLS peptide Deposited 2021-07-14 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 70–529（460 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；296 K；0.65 M Sodium Citrate, 0.1 M HEPES, pH 7.0
Resolution 1.95 Å R-free 0.211
7RG0 Importin alpha2 in complex with MERS ORF4B R24A mutant Deposited 2021-07-14 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 70–529（460 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；296 K；0.6 M Sodium Citrate, 0.1 M HEPES, pH 7.0
Resolution 2.00 Å R-free 0.213
7RG1 Importin alpha2 in complex with MERS ORF4B H26A mutant Deposited 2021-07-14 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 70–529（460 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；296 K；0.7 M Sodium Citrate, 0.1 M HEPES, pH 7.5
Resolution 1.85 Å R-free 0.211
7RG2 Importin alpha2 in complex with MERS ORF4B R33A mutant Deposited 2021-07-14 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 70–529（460 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；296 K；0.7 M Sodium Citrate, 0.1 M HEPES pH 6.5
Resolution 2.00 Å R-free 0.216
7RG3 Importin alpha2 in complex with MERS ORF4B R37A mutant Deposited 2021-07-14 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 70–529（460 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；296 K；0.7 M Sodium Citrate, 0.1 M HEPES, pH 7.5
Resolution 2.00 Å R-free 0.194
7RG4 Importin alpha2 in complex with p50 NLS Deposited 2021-07-14 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain E 70–529（460 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；296 K；0.7 M sodium citrate, 0.01M DTT, 0.1M sodium HEPES pH 7
Resolution 2.60 Å R-free 0.243
7RG6 Importin alpha2 in complex with ORF4B Bat coronavirus HKU5 Deposited 2021-07-14 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 70–529（460 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；296 K；0.1M HEPES pH6.5, 0.6M Citrate, 0.01M DTT
Resolution 2.10 Å R-free 0.223
7TMX Structure of Mouse Importin alpha NEIL1 NLS Peptide Complex Deposited 2022-01-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain I 70–529（460 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；291 K；sodium citrate buffer (pH 6.0), sodium citrate and DTT
Resolution 2.30 Å R-free 0.254
7TMY Structure of Mouse Importin alpha NEIL3 NLS Peptide Complex Deposited 2022-01-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain I 70–529（460 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；291 K；sodium citrate buffer (pH 6.0), sodium citrate and DTT
Resolution 2.21 Å R-free 0.217
7UMI Importin a1 bound to Cp183-CTD Deposited 2022-04-07 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain E 70–529（460 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；0.6 M sodium citrate, 0.1 M HEPES pH 7.2, 10 mM DTT
Resolution 1.99 Å R-free 0.215
8ECH Tick-borne encephalitis virus capsid protein NLS bound to host importin alpha 2 Deposited 2022-09-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain E 70–529（460 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；295.15 K；0.7 M sodium citrate, 0.1 M HEPES, pH 6.5
Resolution 2.05 Å R-free 0.219
8F2Q Human Parvovirus B19 Nonstructural NS1 Protein NLS bound to Importin Alpha 2 Deposited 2022-11-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 70–529（460 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；296 K；0.7M sodium citrate, 0.1M HEPES pH6.5, 0.01M DTT
Resolution 2.70 Å R-free 0.229
8FK3 Adeno-Associated Virus Porcine Origin capsid protein basic regions in complex with Importin-alpha 2 Deposited 2022-12-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 70–529（460 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；0.75M Na Citrate, 0.01M DTT, 0.1M HEPES pH 6.5
Resolution 2.60 Å R-free 0.249
8FUA Crystal structure of mouse Importin alpha in complex with Hendra virus matrix protein NLS1 Deposited 2023-01-17 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 70–529（460 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；0.1M sodium HEPES, 0.72M sodium citrate, 1% dithiothreitol
Resolution 1.90 Å R-free 0.190
8FUC Crystal structure of mouse Importin alpha in complex with Hendra virus matrix protein minor site NLS2 Deposited 2023-01-17 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 70–529（460 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；0.72M sodium citrate, 0.1M sodium HEPES, 1% dithiothreitol
Resolution 2.10 Å R-free 0.217
8G8Q Monopartite p52 NLS in complex with Importin alpha 2 Deposited 2023-02-19 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 70–529（460 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；295 K；0.01M DTT, 0.1M sodium HEPES pH7.5, 0.8.5M sodium citrate
Resolution 2.60 Å R-free 0.210
8G8R RelB NLS in complex with Importin alpha 2 Deposited 2023-02-19 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 70–529（460 aa）
Not recorded DTT 2,3-DIHYDROXY-1,4-DITHIOBUTANE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；295 K；0.01M DTT, 0.1M sodium HEPES pH7.5, 0.8.5M sodium citrate
Resolution 2.60 Å R-free 0.213
8G8S bipartite p52 NLS in complex with Importin alpha 2 Deposited 2023-02-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 70–529（460 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；295 K；0.01M DTT, 0.1M sodium HEPES pH7, 0.6M sodium citrate
Resolution 2.10 Å R-free 0.186
8HE0 Crystal structure of importin-alpha1 bound to the HIF-1alpha nuclear localization signal (wild-type) Deposited 2022-11-07 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 72–498（427 aa） Fragment：UNP residues 72-498
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.7；293 K；tri-sodium citrate, HEPES, DTT
Resolution 1.80 Å R-free 0.198
8HE3 Crystal structure of importin-alpha1 bound to the HIF-1alpha nuclear localization signal (delta 724-751) Deposited 2022-11-07 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 72–498（427 aa） Fragment：UNP residues 72-498
Not recorded SO4 SULFATE ION × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；ammonium sulfate, HEPES, DTT
Resolution 1.90 Å R-free 0.207
8Q8K KI Polyomavirus LTA NLS bound to importin alpha 2 Deposited 2023-08-18 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 70–529（460 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；296 K；0.2 M NaCl, 0.1 M Tris pH 8.0, 16 % PEG4000. Protein stock pre-treated with 5:1 molar ratio IMPa: ivermectin
Resolution 2.70 Å R-free 0.247
8Q8K KI Polyomavirus LTA NLS bound to importin alpha 2 Deposited 2023-08-18 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 70–529（460 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；296 K；0.2 M NaCl, 0.1 M Tris pH 8.0, 16 % PEG4000. Protein stock pre-treated with 5:1 molar ratio IMPa: ivermectin
Resolution 2.70 Å R-free 0.247
8QXW HCMV DNA polymerase processivity factor UL44 unphosphorylated NLS 410-433 bound to mouse importin alpha 2 Deposited 2023-10-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 70–529（460 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；296 K；Sodium citrate 0.65M, HEPES pH7.5, DTT 0.01M
Resolution 2.00 Å R-free 0.221
8QXX HCMV DNA polymerase processivity factor UL44 phosphorylated NLS 410-433 bound to mouse importin alpha 2 Deposited 2023-10-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 70–529（460 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；296 K；Sodium citrate 0.65M, HEPES pH7.0, DTT 0.01M
Resolution 1.90 Å R-free 0.204
8SG7 Adeno-Associated Virus Bat origin capsid protein basic regions in complex with importin-alpha 2 Deposited 2023-04-11 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 70–529（460 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；0.75M Na Citrate, 0.01M DTT, 0.1M HEPES, pH7.5
Resolution 2.40 Å R-free 0.223
8SUD JC Polyomavirus LTA NLS bound to importin alpha 2 Deposited 2023-05-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 70–529（460 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；296 K；0.85 M sodium citrate, 0.1 M HEPES pH 7.5, 0.01 M DTT
Resolution 2.10 Å R-free 0.218
8SV0 The crystal structure of the classical binding interface of Importin alpha 2 and nuclear localisation signal sequence in Psittacine siadenovirus core protein VII Deposited 2023-05-14 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 70–529（460 aa）
Not recorded NA SODIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；296 K；0.6 M sodium citrate, 0.1 M sodium HEPES, 1% dithiothreitol
Resolution 2.20 Å R-free 0.242
8TUQ STL Polyomavirus LTA NLS bound to importin alpha 2 Deposited 2023-08-17 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 70–529（460 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；296 K；0.80 M sodium citrate, 0.1 M HEPES pH 6.5, 0.01 M DTT
Resolution 2.00 Å R-free 0.200
8TUR Merkel Cell Polyomavirus LTA bipartite NLS bound to importin alpha 2 Deposited 2023-08-17 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 70–529（460 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；296 K；0.65 M sodium citrate, 0.1 M HEPES pH 6.5, 0.01 M DTT
Resolution 2.15 Å R-free 0.208
8TUS WU Polyomavirus LTA NLS bound to importin alpha 2 Deposited 2023-08-17 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 70–529（460 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；296 K；0.75 M sodium citrate, 0.1 M HEPES pH 7.0, 0.01 M DTT
Resolution 2.60 Å R-free 0.238
8TUT Merkel Cell Polyomavirus LTA NLSct bound to importin alpha 2 Deposited 2023-08-17 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 70–529（460 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；296 K；0.70 M sodium citrate, 0.10 M HEPES pH 7.0, 0.01 M DTT
Resolution 2.55 Å R-free 0.225
8TUU MW Polyomavirus LTA bipartite NLS bound to importin alpha 2 Deposited 2023-08-17 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 70–529（460 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；296 K；0.7 M sodium citrate, 0.1 M HEPES pH 7.5, 0.01 M DTT
Resolution 2.50 Å R-free 0.235
8TUV Merkel Cell Polyomavirus LTA NLSm bound to importin alpha 2 Deposited 2023-08-17 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 70–529（460 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；296 K；0.75 M sodium citrate, 0.1 M HEPES pH 6.5, 0.01 M DTT
Resolution 2.30 Å R-free 0.218
8U36 The crystal structure of the classical binding interface of Importin alpha 2 and a nuclear localisation signal sequence in Frog siadenovirus core protein VII Deposited 2023-09-07 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 70–529（460 aa） Fragment：UNP residues 70-529
Not recorded NA SODIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；296 K；0.65 M sodium citrate, 0.1 M sodium HEPES, 1% dithiothreitol
Resolution 2.60 Å R-free 0.242
8VPR Crystal structure of mouse Importin alpha in complex with Dengue 1 NS5 central NLS peptide Deposited 2024-01-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 70–529（460 aa） Fragment：UNP residues 70-529
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；296 K；0.75 M sodium citrate, 0.1 M HEPES
Resolution 2.70 Å R-free 0.242
8VPU Crystal structure of mouse Importin alpha in complex with dengue virus 3 NS5 central NLS peptide Deposited 2024-01-17 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 70–529（460 aa） Fragment：UNP residues 70-529
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；296 K；0.75 sodium citrate, 0.1 M HEPES
Resolution 2.10 Å R-free 0.208
9CFT Adeno-associated virus serotype 6 basic regions in complex with importin alpha 2 Deposited 2024-06-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 70–529（460 aa） Fragment：UNP residues 70-529
Not recorded GOL GLYCEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；0.65 M Na citrate, 0.01 M DTT, 0.1 M HEPES, pH 7.0
Resolution 2.30 Å R-free 0.238
9CL8 Importin alpha isoform 2 with synthetic zero net-charge nuclear localization signal Deposited 2024-07-10 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 70–529（460 aa）
Not recorded GOL GLYCEROL × 4 DTT 2,3-DIHYDROXY-1,4-DITHIOBUTANE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.4；296 K；0.8 M NaCit, 0.1M HEPES pH 6.4, 60% glycerol, 10 mM DTT
Resolution 2.11 Å R-free 0.192
9CLE Importin alpha isoform 2 with synthetic zero net-charge nuclear localization signal Deposited 2024-07-11 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 70–529（460 aa）
Not recorded GOL GLYCEROL × 7 DTT 2,3-DIHYDROXY-1,4-DITHIOBUTANE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.6；296 K；0.7 M NaCit, 0.1 M HEPES pH 6.6, 60% glycerol, 10 mM DTT
Resolution 2.34 Å R-free 0.205
9CLF Importin alpha isoform 2 with synthetic zero net-charge nuclear localization signal Deposited 2024-07-11 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 70–529（460 aa）
Not recorded GOL GLYCEROL × 3 DTT 2,3-DIHYDROXY-1,4-DITHIOBUTANE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；296 K；0.65 M NaCit, 0.1 M HEPES pH 7.0, 60% glycerol, 10 mM DTT
Resolution 2.36 Å R-free 0.208
9CLG Importin alpha isoform 2 with synthetic zero net-charge nuclear localization signal Deposited 2024-07-11 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 70–529（460 aa）
Not recorded GOL GLYCEROL × 6 DTT 2,3-DIHYDROXY-1,4-DITHIOBUTANE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.2；296 K；0.8 M NaCit, 0.1 M HEPES pH 6.2, 60% glycerol, 10 mM DTT
Resolution 2.42 Å R-free 0.202
9MIK Gallid alphaherpesvirus-1 large tegument protein bipartite NLS1 in complex with Importin alpha Deposited 2024-12-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain E 70–529（460 aa） Fragment：UNP residues 70-529
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；0.6 M sodium citrate, 0.1 M HEPES
Resolution 2.60 Å R-free 0.212
9MKA Gallid alphaherpesvirus-1 large tegument protein NLS 1 in complex with Importin alpha Deposited 2024-12-16 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain E 71–497（427 aa） Fragment：UNP residues 71-497
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；0.6 M sodium citrate, 0.1 M HEPES
Resolution 2.50 Å R-free 0.208
9N54 Bipartite p63 NLS in complex with Importin Alpha 2 Deposited 2025-02-03 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain E 70–529（460 aa） Fragment：UNP residues 70-529
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；296 K；0.65 M sodium citrate, 0.1 M HEPES and 10 mM DTT
Resolution 2.20 Å R-free 0.206
9NIB Black sea bass polyomavirus LTA NLS bound to improtin alpha 2 Deposited 2025-02-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 70–529（460 aa） Fragment：UNP residues 70-529
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；0.65 M Na citrate, 0.01 M DTT, 0.1 M HEPES, pH 7.0
Resolution 2.20 Å R-free 0.216
9NZ1 Adeno-associated virus serotype 11 basic regions in complex with importin alpha 2 Deposited 2025-03-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 70–529（460 aa） Fragment：UNP residues 70-529
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295 K；0.65 M Na citrate, 0.01 M DTT, 0.1 M HEPES pH 7.0
Resolution 2.68 Å R-free 0.239
9OVZ Gallid alphaherpesvirus-2 large tegument protein NLS in complex with Importin alpha Deposited 2025-06-02 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 70–529（460 aa） Fragment：UNP residues 70-529
Not recorded GOL GLYCEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；0.7 M sodium citrate, 0.1 M HEPES, 0.01 M DTT, pH 7.0
Resolution 2.40 Å R-free 0.225
9PYR Importin alpha 2 in complex with Haliotid herpesvirus 1 large tegument protein NLS region Deposited 2025-08-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain E 70–529（460 aa） Fragment：UNP residues 70-529
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；0.6M sodium citrate, 0.1M HEPES pH 7.0, and 10mM DTT
Resolution 2.60 Å R-free 0.217
9Y0R Importin alpha 2 in complex with ATF2 basic region Deposited 2025-08-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 70–529（460 aa） Fragment：UNP residues 70-529
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；296 K；sodium citrate 0.7 M, 0.1 M HEPES, 10 mM DTT, pH 7
Resolution 2.20 Å R-free 0.222
9Y7K Importin a2 in complex with Guertu virus non-structural S (NSs) NLS peptide Deposited 2025-09-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 70–529（460 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；296 K；0.70 M sodium citrate, 0.01 M DTT, 0.1 M HEPES, pH 7.0
Resolution 2.25 Å R-free 0.205
9YTZ Structure of Ovine gammaherpesvirus 2 ORF73 LANA bound to importin alpha 2 Deposited 2025-10-22 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain E 70–529（460 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；296 K；0.6M sodium citrate, 0.1M HEPES pH 7.0, and 10mM DTT
Resolution 2.85 Å R-free 0.265
9YY4 Importin a2 in complex with Lone Star virus non-structural S (NSs) NLS peptide Deposited 2025-10-28 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 70–529（460 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；0.75 M sodium citrate, 0.01 M DTT, 0.1 M HEPES, pH 7.0
Resolution 2.35 Å R-free 0.229

150 other PDB entries and 151 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name IMA1_MOUSE
Isoform —
PDB entities 2
Chains and sequence ranges Author chain A； PDBConstruct 51–510； UniProt 70–529

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 5u5p

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 5u5p
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2. Structure Basics 2. Structure Basics

Entry ID entry_id5u5p
Deposition date deposition_date2016-12-07
Structure title titleCrystal Structure and X-ray Diffraction Data Collection of Importin-alpha from Mus Musculus Complexed with a MLH1 NLS Peptide
Keywords keywordsNuclear transport, Importin-alpha, MLH1, NLS, TRANSPORT PROTEIN; TRANSPORT PROTEIN
Experimental Method methodX-RAY DIFFRACTION
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier27.73 Å
Radius of gyration Rg (electron density) rg_electron27.56 Å
Forward intensity I(0) i037116100.00
Molecular weight molecular_weight48553.0 kDa
Excluded volume excluded_volume61255 ų
Envelope volume envelope_volume72206 ų
Hydration-shell volume shell_volume23387 ų
Envelope diameter envelope_diameter102.2 Å
Shell Rg shell_rg32.74 Å
Envelope Rg envelope_rg27.78 Å
Shape Rg shape_rg27.59 Å
Total Rg total_rg27.99 Å
Total atoms total_atoms3410
Residues n_residues444
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax98.5 Å
Rg (real space) rg_real28.08 Å
Rg uncertainty (real space) rg_real_error0.86 Å
I(0) (real space) i0_real3.7120e+07
I(0) uncertainty (real space) i0_real_error5.6970e+05
Rg (reciprocal space) rg_reciprocal27.97 Å
I(0) (reciprocal space) i0_reciprocal37110000.0000
Solution quality estimate total_estimate0.5696
Solution quality rating solution_quality REASONABLE a REASONABLE solution
P(r) peaks n_peaks2
Primary peak position r_peak_primary22.5 Å
Skewness Skewness skewness0.556
Kurtosis Kurtosis kurtosis-0.356
Angular range angular_range— – 0.2850 Å−1
Current regularization parameter α current_alpha0.0000
Highest regularization parameter α highest_alpha15920000.0000
Real-space data points n_real_points58
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.591; Stabil: 1.000; Sysdev: 0.075; Positv: 1.000; Valcen: 0.518; Smooth: 0.883
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (4)

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7. Fold Classification (SCOP + CATH) 1 domains

CATH v4.4 (1 domains)

Domain ID domain_id5u5pA00
Class class1 — Mainly Alpha
Architecture architecture25 — Alpha Horseshoe
Topology topology10 — Leucine-rich Repeat Variant
Homologous superfamily homologous superfamily10 — Leucine-rich Repeat Variant
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8. Citations (1)

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9. Files and Curves (10)