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1ejl

MOUSE IMPORTIN ALPHA-SV40 LARGE T ANTIGEN NLS PEPTIDE COMPLEX

Method: X-RAY DIFFRACTION D_max: 98.5 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1ejl
deposition_date	2000-03-03
title	MOUSE IMPORTIN ALPHA-SV40 LARGE T ANTIGEN NLS PEPTIDE COMPLEX
keywords	;importin alpha/karyopherin alpha; nuclear localization sequence (NLS) recognition; simian virus (SV40) large T-antigen, PROTEIN BINDING ;; PROTEIN BINDING
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	28.25 Å
rg_electron	27.94 Å
i0	34942800.00
molecular_weight	47852.0 kDa
excluded_volume	60717 Å³
envelope_volume	72938 Å³
shell_volume	23272 Å³
envelope_diameter	102.2 Å
shell_rg	33.12 Å
envelope_rg	28.09 Å
shape_rg	27.95 Å
total_rg	28.45 Å
total_atoms	3368
n_residues	440
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	98.5 Å
rg_real	28.61 Å
rg_real_error	1.03 Å
i0_real	3.4940e+07
i0_real_error	4.8950e+05
rg_reciprocal	28.50 Å
i0_reciprocal	34940000.0000
total_estimate	0.7933
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	22.9 Å
skewness	0.536
kurtosis	-0.398
angular_range	— – 0.2800 Å⁻¹
current_alpha	0.0000
highest_alpha	12330000.0000
n_real_points	57
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.638; Stabil: 0.994; Sysdev: 1.000; Positv: 1.000; Valcen: 0.510; Smooth: 0.903

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4. Crystallography & Experiment

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5. Entities & Polymer Info (2)

Entity 1 polymer

description	SV40 LARGE T ANTIGEN NLS PEPTIDE
formula_weight	888.2 kDa
src_method	syn
entity_count	2

Polymer

type	polypeptide(L)
strand_id	A,B
atom_count	3368

PKKKRKV

Entity 2 polymer

description	IMPORTIN ALPHA
formula_weight	49886.6 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	I

NQGTVNWSVEDIVKGINSNNLESQLQATQAARKLLSREKQPPIDNIIRAGLIPKFVSFLGKTDCSPIQFESAWALTNIASGTSEQTKAVVDGGAIPAFISLLASPHAHISEQAVWALGNIAGDGSAFRDLVIKHGAIDPLLALLAVPDLSTLACGYLRNLTWTLSNLCRNKNPAPPLDAVEQILPTLVRLLHHNDPEVLADSCWAISYLTDGPNERIEMVVKKGVVPQLVKLLGATELPIVTPALRAIGNIVTGTDEQTQKVIDAGALAVFPSLLTNPKTNIQKEATWTMSNITAGRQDQIQQVVNHGLVPFLVGVLSKADFKTQKEAAWAITNYTSGGTVEQIVYLVHCGIIEPLMNLLSAKDTKIIQVILDAISNIFQAAEKLGETEKLSIMIEECGGLDKIEALQRHENESVYKASLNLIEKYFSVEEEEDQNVVPETTSEGFAFQVQDGAPGTFNF

Source

organism	Mus musculus
common_name	house mouse
ncbi_taxonomy_id	10090
host_organism	Escherichia coli

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6. Citations (2)

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length_a	78.228 Å
length_b	89.858 Å
length_c	99.490 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	8

space_group	P 21 21 21
int_tables_number	19
crystal_system	—

resolution	2.800 Å
resolution_low	30.000 Å
r_factor	0.2150
r_work	0.2150
r_free	0.2540

fit	/app/data/saxs_computation/ej/1ejl/1ejl.fit
pddf	/app/data/pr_computation/ej/1ejl/1ejl.pr.out
profile	/app/data/saxs_computation/ej/1ejl/1ejl.dat

MOUSE IMPORTIN ALPHA-SV40 LARGE T ANTIGEN NLS PEPTIDE COMPLEX

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction

5. Entities & Polymer Info (2)

Entity 1 polymer

Polymer

Entity 2 polymer

Polymer

Source

6. Citations (2)

7. Files & Curves (10)

Files (7)

Curves (3)