8rru

Structure of RyR1 reconstituted into lipid liposomes in primed state in complex with FKBP and Nb9657.

Method: ELECTRON MICROSCOPY D_max: 326.7 Å Quality: EXCELLENT

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1. Protein Identity and Related Structures Protein Identity & Related Structures

Peptidyl-prolyl cis-trans isomerase FKBP1B

OrganismNot specified

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein heterocomplex Heteromer Protein × 12 PDB declaration: 12-meric（12） Consistent with protein copy count	Chain A； UniProt 2–108 Chain D； UniProt 2–108 Chain H； UniProt 2–108 Chain I； UniProt 2–108	Not recorded	Ryanodine receptor 1 × 4 （P11716） Nanobody 9657 × 4 ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 CA CALCIUM ION × 4	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE	Resolution 4.70 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 8RRU	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
8RRT Structure of rabbit RyR1 reconstituted into lipid liposomes in open state in complex with FKBP and Nb9657 Deposited 2024-01-23	Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: 12-meric	Chain A 2–108（107 aa） Chain D 2–108（107 aa） Chain H 2–108（107 aa） Chain I 2–108（107 aa）	Not recorded	ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 CA CALCIUM ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.60 Å
8RRV Structure of RyR1 in detergent in close state in complex with FKBP and Nb9657. Deposited 2024-01-23	Different ligand/ion Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: 12-meric	Chain A 2–108（107 aa） Chain D 2–108（107 aa） Chain H 2–108（107 aa） Chain I 2–108（107 aa）	Not recorded	ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
8RRW Structure of RyR1 in detergent in open state in complex with FKBP and Nb9657. Deposited 2024-01-23	Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: 12-meric	Chain A 2–108（107 aa） Chain D 2–108（107 aa） Chain H 2–108（107 aa） Chain I 2–108（107 aa）	Not recorded	ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 CA CALCIUM ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.20 Å
8RRX Structure of RyR1 reconstituted into lipid nanodisc in primed state in complex with Ca2+, ATP, caffeine and Nb9657 Deposited 2024-01-23	Different ligand/ion Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: 12-meric	Chain F 2–108（107 aa） Chain H 2–108（107 aa） Chain J 2–108（107 aa） Chain L 2–108（107 aa）	Not recorded	ZN ZINC ION × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 CA CALCIUM ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.10 Å
8RS0 Structure of RyR1 in detergent in primed state in complex with nanobody and FKBP Deposited 2024-01-24	Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: 12-meric	Chain A 2–108（107 aa） Chain D 2–108（107 aa） Chain H 2–108（107 aa） Chain I 2–108（107 aa）	Not recorded	ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 CA CALCIUM ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.30 Å
9HEO Open-state RyR1 in 0.05% POPC micelles, in complex with a nanobody and FKBP Deposited 2024-11-14	Different ligand/ion Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: 12-meric	Chain E 2–108（107 aa） Chain F 2–108（107 aa） Chain I 2–108（107 aa） Chain L 2–108（107 aa）	Not recorded	ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 CA CALCIUM ION × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 72	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.40 Å
9HEQ Open-state RyR1 in 0.01% POPC micelles, in complex with a nanobody and FKBP12 Deposited 2024-11-14	Different ligand/ion Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: 12-meric	Chain E 2–108（107 aa） Chain F 2–108（107 aa） Chain I 2–108（107 aa） Chain L 2–108（107 aa）	Not recorded	POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 40 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 CA CALCIUM ION × 4 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.60 Å
9R8O Primed-state RyR1 in 0.05% POPC micelles, in complex with a nanobody and FKBP Deposited 2025-05-16	Different ligand/ion Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: 12-meric	Chain B 2–108（107 aa） Chain E 2–108（107 aa） Chain H 2–108（107 aa） Chain L 2–108（107 aa）	Not recorded	ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 CA CALCIUM ION × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 40 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.30 Å
9RCW Primed-state RyR1 in 0.01% POPC micelles, in complex with a nanobody and FKBP12 Deposited 2025-05-30	Different ligand/ion Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: 12-meric	Chain E 2–108（107 aa） Chain F 2–108（107 aa） Chain I 2–108（107 aa） Chain L 2–108（107 aa）	Not recorded	ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 CA CALCIUM ION × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 24	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.30 Å

9 other PDB entries and 9 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	FKB1B_RABIT
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 1–107； UniProt 2–108 Author chain D； PDBConstruct 1–107； UniProt 2–108 Author chain H； PDBConstruct 1–107； UniProt 2–108 Author chain I； PDBConstruct 1–107； UniProt 2–108

Ryanodine receptor 1

OrganismNot specified

UniProt P11716

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein heterocomplex Heteromer Protein × 12 PDB declaration: 12-meric（12） Consistent with protein copy count	Chain B； UniProt 11–5037 Chain E； UniProt 11–5037 Chain G； UniProt 11–5037 Chain J； UniProt 11–5037	Not recorded	Peptidyl-prolyl cis-trans isomerase FKBP1B × 4 （Q8HYX6） Nanobody 9657 × 4 ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 CA CALCIUM ION × 4	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE	Resolution 4.70 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 8RRU	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
2BCX Crystal structure of calmodulin in complex with a ryanodine receptor peptide Deposited 2005-10-19	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain B 3614–3643（30 aa） Fragment：residues 3614-3643	Not recorded	CA CALCIUM ION × 4	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4.7；293 K；23% PEG 4000, 0.1 M sodium acetate, 5 mM DTT, pH 4.7, VAPOR DIFFUSION, SITTING DROP, temperature 293K	Resolution 2.00 Å R-free 0.249
2XOA Crystal Structure of the N-terminal three domains of the skeletal muscle Ryanodine Receptor (RyR1) Deposited 2010-08-10	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1–559（559 aa） Fragment：N-TERMINAL DISEASE HOT SPOT, RESIDUES 1-559	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions pH 8；pH 8	Resolution 2.50 Å R-free 0.234
3HSM Crystal structure of distal N-terminal beta-trefoil domain of Ryanodine Receptor type 1 Deposited 2009-06-10	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1–210（210 aa） Fragment：sequence database residues 1-210	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；298 K；0.1M MES, 0.1M MgCl2, 24% PEG 3350, 5mM DTT, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K	Resolution 2.50 Å R-free 0.276
3HSM Crystal structure of distal N-terminal beta-trefoil domain of Ryanodine Receptor type 1 Deposited 2009-06-10	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain B 1–210（210 aa） Fragment：sequence database residues 1-210	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；298 K；0.1M MES, 0.1M MgCl2, 24% PEG 3350, 5mM DTT, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K	Resolution 2.50 Å R-free 0.276
3ILA Crystal structure of rabbit ryanodine receptor 1 N-terminal domain (9-205) Deposited 2009-08-06	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 9–205（197 aa） Fragment：N-Terminal Domain	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295 K；15-25% PEG 3350, 0.2M sodium chloride, 0.1M BisTris, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K	Resolution 2.90 Å R-free 0.320
3ILA Crystal structure of rabbit ryanodine receptor 1 N-terminal domain (9-205) Deposited 2009-08-06	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain B 9–205（197 aa） Fragment：N-Terminal Domain	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295 K；15-25% PEG 3350, 0.2M sodium chloride, 0.1M BisTris, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K	Resolution 2.90 Å R-free 0.320
3ILA Crystal structure of rabbit ryanodine receptor 1 N-terminal domain (9-205) Deposited 2009-08-06	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain C 9–205（197 aa） Fragment：N-Terminal Domain	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295 K；15-25% PEG 3350, 0.2M sodium chloride, 0.1M BisTris, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K	Resolution 2.90 Å R-free 0.320
3ILA Crystal structure of rabbit ryanodine receptor 1 N-terminal domain (9-205) Deposited 2009-08-06	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain D 9–205（197 aa） Fragment：N-Terminal Domain	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295 K；15-25% PEG 3350, 0.2M sodium chloride, 0.1M BisTris, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K	Resolution 2.90 Å R-free 0.320
3ILA Crystal structure of rabbit ryanodine receptor 1 N-terminal domain (9-205) Deposited 2009-08-06	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 5 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain E 9–205（197 aa） Fragment：N-Terminal Domain	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295 K；15-25% PEG 3350, 0.2M sodium chloride, 0.1M BisTris, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K	Resolution 2.90 Å R-free 0.320
3ILA Crystal structure of rabbit ryanodine receptor 1 N-terminal domain (9-205) Deposited 2009-08-06	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 6 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain F 9–205（197 aa） Fragment：N-Terminal Domain	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295 K；15-25% PEG 3350, 0.2M sodium chloride, 0.1M BisTris, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K	Resolution 2.90 Å R-free 0.320
3ILA Crystal structure of rabbit ryanodine receptor 1 N-terminal domain (9-205) Deposited 2009-08-06	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 7 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain G 9–205（197 aa） Fragment：N-Terminal Domain	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295 K；15-25% PEG 3350, 0.2M sodium chloride, 0.1M BisTris, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K	Resolution 2.90 Å R-free 0.320
3ILA Crystal structure of rabbit ryanodine receptor 1 N-terminal domain (9-205) Deposited 2009-08-06	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 8 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain H 9–205（197 aa） Fragment：N-Terminal Domain	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295 K；15-25% PEG 3350, 0.2M sodium chloride, 0.1M BisTris, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K	Resolution 2.90 Å R-free 0.320
3ILA Crystal structure of rabbit ryanodine receptor 1 N-terminal domain (9-205) Deposited 2009-08-06	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 9 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain I 9–205（197 aa） Fragment：N-Terminal Domain	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295 K；15-25% PEG 3350, 0.2M sodium chloride, 0.1M BisTris, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K	Resolution 2.90 Å R-free 0.320
3J8H Structure of the rabbit ryanodine receptor RyR1 in complex with FKBP12 at 3.8 Angstrom resolution Deposited 2014-10-26	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain A 1–1491（1491 aa） Chain A 1510–1976（467 aa） Chain A 2057–2460（404 aa） Chain A 2494–2562（69 aa） Chain A 2734–2940（207 aa） Chain A 3645–5037（1393 aa） Chain C 1–1491（1491 aa） Chain C 1510–1976（467 aa） Chain C 2057–2460（404 aa） Chain C 2494–2562（69 aa） Chain C 2734–2940（207 aa） Chain C 3645–5037（1393 aa） Chain E 1–1491（1491 aa） Chain E 1510–1976（467 aa） Chain E 2057–2460（404 aa） Chain E 2494–2562（69 aa） Chain E 2734–2940（207 aa） Chain E 3645–5037（1393 aa） Chain G 1–1491（1491 aa） Chain G 1510–1976（467 aa） Chain G 2057–2460（404 aa） Chain G 2494–2562（69 aa） Chain G 2734–2940（207 aa） Chain G 3645–5037（1393 aa）	Not recorded	ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer 20 mM MOPS sodium, pH 7.4, 250 mM NaCl, 2 mM DTT, 2 mM EGTA, 0.015% Tween20, protein inhibitors；pH 7.4；20 mM MOPS sodium, pH 7.4, 250 mM NaCl, 2 mM DTT, 2 mM EGTA, 0.015% Tween20, protein inhibitors cryo-EM vitrification conditions protein solution was applied to glow-discharged carbon coated grid and blotted for 2 seconds before plunging；Cryogen NITROGEN；Protein solution was applied to glow-discharged carbon coated grid and blotted for 2 seconds before plunging into liquid nitrogen (FEI VITROBOT MARK III)	Resolution 3.80 Å
3RQR Crystal structure of the RYR domain of the rabbit ryanodine receptor Deposited 2011-04-28	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain A 2733–2940（208 aa） Fragment：Residues 2733-2940	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions pH 8.5；291 K；1.2M Na Citrate, 100mM Tris pH8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K	Resolution 2.16 Å R-free 0.258
4ERT Crystal structure of rabbit ryanodine receptor 1 (2734-2940) Deposited 2012-04-20	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 2734–2940（207 aa）	Not recorded	GOL GLYCEROL × 2 K POTASSIUM ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295 K；19% PEG3350, 30% Glycerol, 0.1M HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K	Resolution 1.95 Å R-free 0.225
4ESU Crystal structure of rabbit ryanodine receptor 1 mutant S2776M Deposited 2012-04-23	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 2734–2940（207 aa）	Mutation：S2776M	GOL GLYCEROL × 5	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295 K；25% PEG3350, 25% Glycerol, 0.1M HEPES, pH 7.0, vapor diffusion, hanging drop, temperature 295K	Resolution 1.59 Å R-free 0.229
4ETT Crystal structure of rabbit ryanodine receptor 1 mutant E2764K Deposited 2012-04-24	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 2734–2940（207 aa）	Mutation：E2764K	GOL GLYCEROL × 4	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295 K；25% PEG3350, 25% Glycerol, 0.1M HEPES, pH 7.0, vapor diffusion, hanging drop, temperature 295K	Resolution 2.19 Å R-free 0.287
4ETU Crystal structure of rabbit ryanodine receptor 1 mutant R2939S Deposited 2012-04-24	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 2734–2940（207 aa）	Mutation：R2939S	GOL GLYCEROL × 3	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295 K；15% PEG3350, 25% Glycerol, 0.1M HEPES, pH 7.0, vapor diffusion, hanging drop, temperature 295K	Resolution 2.19 Å R-free 0.262
4I0Y CRYSTAL STRUCTURE OF RABBIT RYANODINE RECEPTOR 1 (RESIDUES 1-536) DISEASE MUTANT C36R Deposited 2012-11-19	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1–536（536 aa） Fragment：N-TERMINAL DISEASE HOT SPOT, RESIDUES 1-536	Mutation：C36R	GOL GLYCEROL × 1	X-RAY DIFFRACTION X-ray crystallization conditions EVAPORATION；pH 9.5；pH 9.5, EVAPORATION	Resolution 2.80 Å R-free 0.246
4I1E Crystal structure of Rabbit Ryanodine Receptor 1 (residues 1-536) disease mutant G249R Deposited 2012-11-20	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1–536（536 aa） Fragment：N-TERMINAL DISEASE HOT SPOT, RESIDUES 1-536	Mutation：G249R	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions EVAPORATION；pH 8；pH 8.0, EVAPORATION	Resolution 2.40 Å R-free 0.258
4I2S Crystal structure of rabbit ryanodine receptor 1 (residues 1-536) disease mutant I404M Deposited 2012-11-22	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1–536（536 aa） Fragment：N-TERMINAL DISEASE HOT SPOT, RESIDUES 1-536	Mutation：I404M	GOL GLYCEROL × 1	X-RAY DIFFRACTION X-ray crystallization conditions EVAPORATION；pH 8；pH 8.0, EVAPORATION	Resolution 2.50 Å R-free 0.249
4I37 Crystal structure of rabbit ryanodine receptor 1 (residues 1-536) disease mutant R402G Deposited 2012-11-25	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1–536（536 aa） Fragment：N-TERMINAL DISEASE HOT SPOT, RESIDUES 1-536	Mutation：R402G	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions EVAPORATION；pH 6.5；pH 6.5, EVAPORATION	Resolution 2.95 Å R-free 0.289
4I3N Crystal structure of rabbit ryanodine receptor 1 (residues 1-536) disease mutant D61N Deposited 2012-11-26	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1–536（536 aa） Fragment：N-TERMINAL DISEASE HOT SPOT, RESIDUES 1-536	Mutation：D61N	GOL GLYCEROL × 1	X-RAY DIFFRACTION X-ray crystallization conditions EVAPORATION；pH 9.5；pH 9.5, EVAPORATION	Resolution 2.95 Å R-free 0.257
4I6I Crystal structure of rabbit ryanodine receptor 1 (residues 1-559) disease mutant R45C Deposited 2012-11-29	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1–559（559 aa） Fragment：N-TERMINAL DISEASE HOT SPOT, RESIDUES 1-559	Mutation：R45C	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions EVAPORATION；pH 7；pH 7.0, EVAPORATION	Resolution 2.50 Å R-free 0.277
4I7I Crystal structure of rabbit ryanodine receptor 1 (residues 1-536) disease mutant L14R Deposited 2012-11-30	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1–536（536 aa） Fragment：N-TERMINAL DISEASE HOT SPOT, RESIDUES 1-536	Mutation：L14R	GOL GLYCEROL × 1	X-RAY DIFFRACTION X-ray crystallization conditions EVAPORATION；pH 8；pH 8.0, EVAPORATION	Resolution 2.90 Å R-free 0.249
4I8M Crystal structure of rabbit Ryanodine receptor 1 (residues 1-536) disease mutant V219I Deposited 2012-12-03	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1–536（536 aa） Fragment：N-TERMINAL DISEASE HOT SPOT, RESIDUES 1-536	Mutation：V219I	GOL GLYCEROL × 1	X-RAY DIFFRACTION X-ray crystallization conditions EVAPORATION；pH 9；pH 9.0, EVAPORATION	Resolution 2.80 Å R-free 0.253
4I96 Crystal structure of the N-terminal two domains of the skeletal muscle ryanodine receptor (rabbit RyR1) residues 217-536 Deposited 2012-12-04	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 217–536（320 aa） Fragment：N-TERMINAL DISEASE HOT SPOT, RESIDUES 217-536	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions EVAPORATION；pH 8；pH 8.0, EVAPORATION	Resolution 2.73 Å R-free 0.241
4P9J Crystal Structure of rabbit Ryanodine Receptor 1 SPRY2 Domain (1070-1246) Deposited 2014-04-04	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain C 1070–1246（177 aa） Fragment：UNP Residues 1070-1246	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；298.15 K；sodium citrate	Resolution 1.84 Å R-free 0.220
4P9J Crystal Structure of rabbit Ryanodine Receptor 1 SPRY2 Domain (1070-1246) Deposited 2014-04-04	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1070–1246（177 aa） Fragment：UNP Residues 1070-1246	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；298.15 K；sodium citrate	Resolution 1.84 Å R-free 0.220
4P9J Crystal Structure of rabbit Ryanodine Receptor 1 SPRY2 Domain (1070-1246) Deposited 2014-04-04	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain B 1070–1246（177 aa） Fragment：UNP Residues 1070-1246	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；298.15 K；sodium citrate	Resolution 1.84 Å R-free 0.220
4UWA Structure of the ryanodine receptor at resolution of 6.1 A in closed state Deposited 2014-08-11	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–533（533 aa） Chain A 649–1571（923 aa） Chain A 2734–2939（206 aa） Chain B 1–533（533 aa） Chain B 649–1571（923 aa） Chain B 2734–2939（206 aa） Chain C 1–533（533 aa） Chain C 649–1571（923 aa） Chain C 2734–2939（206 aa） Chain D 1–533（533 aa） Chain D 649–1571（923 aa） Chain D 2734–2939（206 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer 10 MM MOPS, 200MM NACL, 2MM DTT, 1MM EGTA；pH 7.4；10 MM MOPS, 200MM NACL, 2MM DTT, 1MM EGTA cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE	Resolution 6.10 Å
4UWE Structure of the ryanodine receptor at resolution of 8.5 A in partially open state Deposited 2014-08-11	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–533（533 aa） Chain A 649–1571（923 aa） Chain A 2734–2939（206 aa） Chain B 1–533（533 aa） Chain B 649–1571（923 aa） Chain B 2734–2939（206 aa） Chain C 1–533（533 aa） Chain C 649–1571（923 aa） Chain C 2734–2939（206 aa） Chain D 1–533（533 aa） Chain D 649–1571（923 aa） Chain D 2734–2939（206 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer 10 MM MOPS, 200 MM NACL, 2 MM DTT AND 10 MM CACL2, 0.2% FLUORINATED OCTYL- MALTOSIDE；pH 7.4；10 MM MOPS, 200 MM NACL, 2 MM DTT AND 10 MM CACL2, 0.2% FLUORINATED OCTYL- MALTOSIDE cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE	Resolution 8.50 Å
5C30 Crystal Structure of Rabbit Ryanodine Receptor 1 Repeat12 Domain Deposited 2015-06-16	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 857–1054（198 aa） Fragment：repeat12 domain (UNP residues 857-1054)	Mutation：C906A, C937A, C1040A	IPA ISOPROPYL ALCOHOL × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；0.1 M HEPES, pH 7.0, 19% w/v PEG20000	Resolution 1.55 Å R-free 0.165
5GKY Structure of RyR1 in a closed state (C1 conformer) Deposited 2016-07-07	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain A 1–5037（5037 aa） Chain C 1–5037（5037 aa） Chain E 1–5037（5037 aa） Chain G 1–5037（5037 aa）	Not recorded	ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4；20 mM MOPS-Na, pH 7.4, 250 mM NaCl, 2 mM DTT, 0.015% Tween20 (w/v) (Sigma-Aldrich) and protease inhibitor cocktail including 2 mM PMSF, 2.6 ug/ml aprotinin, 1.4 ug/ml pepstatin, and 10 ug/ml leupeptin (Amresco) cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.80 Å
5GKZ Structure of RyR1 in a closed state (C3 conformer) Deposited 2016-07-07	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain A 1–5037（5037 aa） Chain C 1–5037（5037 aa） Chain E 1–5037（5037 aa） Chain G 1–5037（5037 aa）	Not recorded	ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4；20 mM MOPS-Na, pH 7.4, 250 mM NaCl, 2 mM DTT, 0.015% Tween20 (w/v) (Sigma-Aldrich) and protease inhibitor cocktail including 2 mM PMSF, 2.6 ug/ml aprotinin, 1.4 ug/ml pepstatin, and 10 ug/ml leupeptin (Amresco) cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.00 Å
5GL0 Structure of RyR1 in a closed state (C4 conformer) Deposited 2016-07-07	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain A 1–5037（5037 aa） Chain C 1–5037（5037 aa） Chain E 1–5037（5037 aa） Chain G 1–5037（5037 aa）	Not recorded	ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4；20 mM MOPS-Na, pH 7.4, 250 mM NaCl, 2 mM DTT, 0.015% Tween20 (w/v) (Sigma-Aldrich) and protease inhibitor cocktail including 2 mM PMSF, 2.6 ug/ml aprotinin, 1.4 ug/ml pepstatin, and 10 ug/ml leupeptin (Amresco) cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.20 Å
5GL1 Structure of RyR1 in an open state Deposited 2016-07-07	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain A 1–5037（5037 aa） Chain C 1–5037（5037 aa） Chain E 1–5037（5037 aa） Chain G 1–5037（5037 aa）	Not recorded	ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4；20 mM MOPS-Na, pH 7.4, 250 mM NaCl, 2 mM DTT, 0.5% CHAPS (w/v) (Sigma-Aldrich) and protease inhibitor cocktail including 2 mM PMSF, 2.6 ug/ml aprotinin, 1.4 ug/ml pepstatin, and 10 ug/ml leupeptin (Amresco),50 uM CaCl2 and 10 uM PCB95 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 5.70 Å
5J8V Structure of rabbit ryanodine receptor RyR1 open state activated by calcium ion Deposited 2016-04-08	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–5037（5037 aa） Chain B 1–5037（5037 aa） Chain C 1–5037（5037 aa） Chain D 1–5037（5037 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4；1:1000 diluted protease inhibitor cocktail was also added in buffer cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2s before plunging.	Resolution 4.90 Å
5T15 Structural basis for gating and activation of RyR1 (30 uM Ca2+ dataset, all particles) Deposited 2016-08-17	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain B 12–1275（1264 aa） Chain B 1573–2479（907 aa） Chain B 2734–2939（206 aa） Chain B 3639–5037（1399 aa） Chain E 12–1275（1264 aa） Chain E 1573–2479（907 aa） Chain E 2734–2939（206 aa） Chain E 3639–5037（1399 aa） Chain G 12–1275（1264 aa） Chain G 1573–2479（907 aa） Chain G 2734–2939（206 aa） Chain G 3639–5037（1399 aa） Chain I 12–1275（1264 aa） Chain I 1573–2479（907 aa） Chain I 2734–2939（206 aa） Chain I 3639–5037（1399 aa）	Not recorded	ZN ZINC ION × 4 CA CALCIUM ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds	Resolution 3.60 Å
5T9M Structure of rabbit RyR1 (Ca2+-only dataset, class 1) Deposited 2016-09-09	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain B 12–1275（1264 aa） Chain B 1573–2479（907 aa） Chain B 2734–2939（206 aa） Chain B 3639–5037（1399 aa） Chain E 12–1275（1264 aa） Chain E 1573–2479（907 aa） Chain E 2734–2939（206 aa） Chain E 3639–5037（1399 aa） Chain G 12–1275（1264 aa） Chain G 1573–2479（907 aa） Chain G 2734–2939（206 aa） Chain G 3639–5037（1399 aa） Chain I 12–1275（1264 aa） Chain I 1573–2479（907 aa） Chain I 2734–2939（206 aa） Chain I 3639–5037（1399 aa）	Not recorded	ZN ZINC ION × 4 CA CALCIUM ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds	Resolution 4.00 Å
5T9N Structure of rabbit RyR1 (Ca2+-only dataset, class 2) Deposited 2016-09-09	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain B 12–1275（1264 aa） Chain B 1573–2479（907 aa） Chain B 2734–2939（206 aa） Chain B 3639–5037（1399 aa） Chain E 12–1275（1264 aa） Chain E 1573–2479（907 aa） Chain E 2734–2939（206 aa） Chain E 3639–5037（1399 aa） Chain G 12–1275（1264 aa） Chain G 1573–2479（907 aa） Chain G 2734–2939（206 aa） Chain G 3639–5037（1399 aa） Chain I 12–1275（1264 aa） Chain I 1573–2479（907 aa） Chain I 2734–2939（206 aa） Chain I 3639–5037（1399 aa）	Not recorded	ZN ZINC ION × 4 CA CALCIUM ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds	Resolution 3.80 Å
5T9R Structure of rabbit RyR1 (Ca2+-only dataset, class 3) Deposited 2016-09-09	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain B 12–1275（1264 aa） Chain B 1573–2479（907 aa） Chain B 2734–2939（206 aa） Chain B 3639–5037（1399 aa） Chain E 12–1275（1264 aa） Chain E 1573–2479（907 aa） Chain E 2734–2939（206 aa） Chain E 3639–5037（1399 aa） Chain G 12–1275（1264 aa） Chain G 1573–2479（907 aa） Chain G 2734–2939（206 aa） Chain G 3639–5037（1399 aa） Chain I 12–1275（1264 aa） Chain I 1573–2479（907 aa） Chain I 2734–2939（206 aa） Chain I 3639–5037（1399 aa）	Not recorded	ZN ZINC ION × 4 CA CALCIUM ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds	Resolution 5.80 Å
5T9S Structure of rabbit RyR1 (Ca2+-only dataset, class 4) Deposited 2016-09-09	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain B 12–1275（1264 aa） Chain B 1573–2479（907 aa） Chain B 2734–2939（206 aa） Chain B 3639–5037（1399 aa） Chain E 12–1275（1264 aa） Chain E 1573–2479（907 aa） Chain E 2734–2939（206 aa） Chain E 3639–5037（1399 aa） Chain G 12–1275（1264 aa） Chain G 1573–2479（907 aa） Chain G 2734–2939（206 aa） Chain G 3639–5037（1399 aa） Chain I 12–1275（1264 aa） Chain I 1573–2479（907 aa） Chain I 2734–2939（206 aa） Chain I 3639–5037（1399 aa）	Not recorded	ZN ZINC ION × 4 CA CALCIUM ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds	Resolution 4.20 Å
5T9V Structure of rabbit RyR1 (Caffeine/ATP/Ca2+ dataset, class 1) Deposited 2016-09-09	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain B 12–1275（1264 aa） Chain B 1573–2479（907 aa） Chain B 2734–2939（206 aa） Chain B 3639–4253（615 aa） Chain B 4540–5037（498 aa） Chain E 12–1275（1264 aa） Chain E 1573–2479（907 aa） Chain E 2734–2939（206 aa） Chain E 3639–4253（615 aa） Chain E 4540–5037（498 aa） Chain G 12–1275（1264 aa） Chain G 1573–2479（907 aa） Chain G 2734–2939（206 aa） Chain G 3639–4253（615 aa） Chain G 4540–5037（498 aa） Chain I 12–1275（1264 aa） Chain I 1573–2479（907 aa） Chain I 2734–2939（206 aa） Chain I 3639–4253（615 aa） Chain I 4540–5037（498 aa）	Not recorded	ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds	Resolution 4.40 Å
5TA3 Structure of rabbit RyR1 (Caffeine/ATP/Ca2+ dataset, class 2) Deposited 2016-09-09	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain B 12–1275（1264 aa） Chain B 1573–2479（907 aa） Chain B 2734–2939（206 aa） Chain B 3639–4253（615 aa） Chain B 4541–5037（497 aa） Chain E 12–1275（1264 aa） Chain E 1573–2479（907 aa） Chain E 2734–2939（206 aa） Chain E 3639–4253（615 aa） Chain E 4541–5037（497 aa） Chain G 12–1275（1264 aa） Chain G 1573–2479（907 aa） Chain G 2734–2939（206 aa） Chain G 3639–4253（615 aa） Chain G 4541–5037（497 aa） Chain I 12–1275（1264 aa） Chain I 1573–2479（907 aa） Chain I 2734–2939（206 aa） Chain I 3639–4253（615 aa） Chain I 4541–5037（497 aa）	Not recorded	ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds	Resolution 4.40 Å
5TAL Structure of rabbit RyR1 (Caffeine/ATP/Ca2+ dataset, class 1&2) Deposited 2016-09-10	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain B 12–1275（1264 aa） Chain B 1573–2479（907 aa） Chain B 2734–2939（206 aa） Chain B 3639–4253（615 aa） Chain B 4541–5037（497 aa） Chain E 12–1275（1264 aa） Chain E 1573–2479（907 aa） Chain E 2734–2939（206 aa） Chain E 3639–4253（615 aa） Chain E 4541–5037（497 aa） Chain G 12–1275（1264 aa） Chain G 1573–2479（907 aa） Chain G 2734–2939（206 aa） Chain G 3639–4253（615 aa） Chain G 4541–5037（497 aa） Chain I 12–1275（1264 aa） Chain I 1573–2479（907 aa） Chain I 2734–2939（206 aa） Chain I 3639–4253（615 aa） Chain I 4541–5037（497 aa）	Not recorded	ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds	Resolution 4.30 Å
5TAM Structure of rabbit RyR1 (Caffeine/ATP/Ca2+ dataset, class 4) Deposited 2016-09-10	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain B 12–1275（1264 aa） Chain B 1573–2479（907 aa） Chain B 2734–2939（206 aa） Chain B 3639–4253（615 aa） Chain B 4541–5037（497 aa） Chain E 12–1275（1264 aa） Chain E 1573–2479（907 aa） Chain E 2734–2939（206 aa） Chain E 3639–4253（615 aa） Chain E 4541–5037（497 aa） Chain G 12–1275（1264 aa） Chain G 1573–2479（907 aa） Chain G 2734–2939（206 aa） Chain G 3639–4253（615 aa） Chain G 4541–5037（497 aa） Chain I 12–1275（1264 aa） Chain I 1573–2479（907 aa） Chain I 2734–2939（206 aa） Chain I 3639–4253（615 aa） Chain I 4541–5037（497 aa）	Not recorded	ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds	Resolution 4.30 Å
5TAN Structure of rabbit RyR1 (Caffeine/ATP/Ca2+ dataset, class 3) Deposited 2016-09-10	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain B 12–1275（1264 aa） Chain B 1573–2479（907 aa） Chain B 2734–2939（206 aa） Chain B 3639–4253（615 aa） Chain B 4541–5037（497 aa） Chain E 12–1275（1264 aa） Chain E 1573–2479（907 aa） Chain E 2734–2939（206 aa） Chain E 3639–4253（615 aa） Chain E 4541–5037（497 aa） Chain G 12–1275（1264 aa） Chain G 1573–2479（907 aa） Chain G 2734–2939（206 aa） Chain G 3639–4253（615 aa） Chain G 4541–5037（497 aa） Chain I 12–1275（1264 aa） Chain I 1573–2479（907 aa） Chain I 2734–2939（206 aa） Chain I 3639–4253（615 aa） Chain I 4541–5037（497 aa）	Not recorded	ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds	Resolution 4.30 Å
5TAP Structure of rabbit RyR1 (Caffeine/ATP/EGTA dataset, all particles) Deposited 2016-09-10	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain B 12–1275（1264 aa） Chain B 1573–2479（907 aa） Chain B 2734–2939（206 aa） Chain B 3639–4253（615 aa） Chain B 4541–5037（497 aa） Chain E 12–1275（1264 aa） Chain E 1573–2479（907 aa） Chain E 2734–2939（206 aa） Chain E 3639–4253（615 aa） Chain E 4541–5037（497 aa） Chain G 12–1275（1264 aa） Chain G 1573–2479（907 aa） Chain G 2734–2939（206 aa） Chain G 3639–4253（615 aa） Chain G 4541–5037（497 aa） Chain I 12–1275（1264 aa） Chain I 1573–2479（907 aa） Chain I 2734–2939（206 aa） Chain I 3639–4253（615 aa） Chain I 4541–5037（497 aa）	Not recorded	ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds	Resolution 4.40 Å
5TAQ Structure of rabbit RyR1 (Caffeine/ATP/Ca2+ dataset, class 3&4) Deposited 2016-09-10	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain B 12–1275（1264 aa） Chain B 1573–2479（907 aa） Chain B 2734–2939（206 aa） Chain B 3639–4253（615 aa） Chain B 4541–5037（497 aa） Chain E 12–1275（1264 aa） Chain E 1573–2479（907 aa） Chain E 2734–2939（206 aa） Chain E 3639–4253（615 aa） Chain E 4541–5037（497 aa） Chain G 12–1275（1264 aa） Chain G 1573–2479（907 aa） Chain G 2734–2939（206 aa） Chain G 3639–4253（615 aa） Chain G 4541–5037（497 aa） Chain I 12–1275（1264 aa） Chain I 1573–2479（907 aa） Chain I 2734–2939（206 aa） Chain I 3639–4253（615 aa） Chain I 4541–5037（497 aa）	Not recorded	ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds	Resolution 4.10 Å
5TAS Structure of rabbit RyR1 (Caffeine/ATP/EGTA dataset, class 1) Deposited 2016-09-10	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain B 12–1275（1264 aa） Chain B 1573–2479（907 aa） Chain B 2734–2939（206 aa） Chain B 3639–4253（615 aa） Chain B 4541–5037（497 aa） Chain E 12–1275（1264 aa） Chain E 1573–2479（907 aa） Chain E 2734–2939（206 aa） Chain E 3639–4253（615 aa） Chain E 4541–5037（497 aa） Chain G 12–1275（1264 aa） Chain G 1573–2479（907 aa） Chain G 2734–2939（206 aa） Chain G 3639–4253（615 aa） Chain G 4541–5037（497 aa） Chain I 12–1275（1264 aa） Chain I 1573–2479（907 aa） Chain I 2734–2939（206 aa） Chain I 3639–4253（615 aa） Chain I 4541–5037（497 aa）	Not recorded	ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds	Resolution 6.20 Å
5TAT Structure of rabbit RyR1 (Caffeine/ATP/EGTA dataset, class 2) Deposited 2016-09-10	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain B 12–1275（1264 aa） Chain B 1573–2479（907 aa） Chain B 2734–2939（206 aa） Chain B 3639–4253（615 aa） Chain B 4541–5037（497 aa） Chain E 12–1275（1264 aa） Chain E 1573–2479（907 aa） Chain E 2734–2939（206 aa） Chain E 3639–4253（615 aa） Chain E 4541–5037（497 aa） Chain G 12–1275（1264 aa） Chain G 1573–2479（907 aa） Chain G 2734–2939（206 aa） Chain G 3639–4253（615 aa） Chain G 4541–5037（497 aa） Chain I 12–1275（1264 aa） Chain I 1573–2479（907 aa） Chain I 2734–2939（206 aa） Chain I 3639–4253（615 aa） Chain I 4541–5037（497 aa）	Not recorded	ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds	Resolution 4.80 Å
5TAU Structure of rabbit RyR1 (Caffeine/ATP/EGTA dataset, class 3) Deposited 2016-09-10	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain B 12–1275（1264 aa） Chain B 1573–2479（907 aa） Chain B 2734–2939（206 aa） Chain B 3639–4253（615 aa） Chain B 4541–5037（497 aa） Chain E 12–1275（1264 aa） Chain E 1573–2479（907 aa） Chain E 2734–2939（206 aa） Chain E 3639–4253（615 aa） Chain E 4541–5037（497 aa） Chain G 12–1275（1264 aa） Chain G 1573–2479（907 aa） Chain G 2734–2939（206 aa） Chain G 3639–4253（615 aa） Chain G 4541–5037（497 aa） Chain I 12–1275（1264 aa） Chain I 1573–2479（907 aa） Chain I 2734–2939（206 aa） Chain I 3639–4253（615 aa） Chain I 4541–5037（497 aa）	Not recorded	ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds	Resolution 6.20 Å
5TAV Structure of rabbit RyR1 (Caffeine/ATP/EGTA dataset, class 4) Deposited 2016-09-10	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain B 12–1275（1264 aa） Chain B 1573–2479（907 aa） Chain B 2734–2939（206 aa） Chain B 3639–4253（615 aa） Chain B 4541–5037（497 aa） Chain E 12–1275（1264 aa） Chain E 1573–2479（907 aa） Chain E 2734–2939（206 aa） Chain E 3639–4253（615 aa） Chain E 4541–5037（497 aa） Chain G 12–1275（1264 aa） Chain G 1573–2479（907 aa） Chain G 2734–2939（206 aa） Chain G 3639–4253（615 aa） Chain G 4541–5037（497 aa） Chain I 12–1275（1264 aa） Chain I 1573–2479（907 aa） Chain I 2734–2939（206 aa） Chain I 3639–4253（615 aa） Chain I 4541–5037（497 aa）	Not recorded	ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds	Resolution 4.80 Å
5TAW Structure of rabbit RyR1 (ryanodine dataset, all particles) Deposited 2016-09-10	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain B 12–1275（1264 aa） Chain B 1573–2479（907 aa） Chain B 2734–2939（206 aa） Chain B 3639–4253（615 aa） Chain B 4541–5037（497 aa） Chain E 12–1275（1264 aa） Chain E 1573–2479（907 aa） Chain E 2734–2939（206 aa） Chain E 3639–4253（615 aa） Chain E 4541–5037（497 aa） Chain G 12–1275（1264 aa） Chain G 1573–2479（907 aa） Chain G 2734–2939（206 aa） Chain G 3639–4253（615 aa） Chain G 4541–5037（497 aa） Chain I 12–1275（1264 aa） Chain I 1573–2479（907 aa） Chain I 2734–2939（206 aa） Chain I 3639–4253（615 aa） Chain I 4541–5037（497 aa）	Not recorded	ZN ZINC ION × 4 CA CALCIUM ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds	Resolution 4.40 Å
5TAX Structure of rabbit RyR1 (ryanodine dataset, class 1) Deposited 2016-09-10	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain B 12–1275（1264 aa） Chain B 1573–2479（907 aa） Chain B 2734–2939（206 aa） Chain B 3639–4253（615 aa） Chain B 4541–5037（497 aa） Chain E 12–1275（1264 aa） Chain E 1573–2479（907 aa） Chain E 2734–2939（206 aa） Chain E 3639–4253（615 aa） Chain E 4541–5037（497 aa） Chain G 12–1275（1264 aa） Chain G 1573–2479（907 aa） Chain G 2734–2939（206 aa） Chain G 3639–4253（615 aa） Chain G 4541–5037（497 aa） Chain I 12–1275（1264 aa） Chain I 1573–2479（907 aa） Chain I 2734–2939（206 aa） Chain I 3639–4253（615 aa） Chain I 4541–5037（497 aa）	Not recorded	ZN ZINC ION × 4 CA CALCIUM ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds	Resolution 6.60 Å
5TAY Structure of rabbit RyR1 (ryanodine dataset, class 2) Deposited 2016-09-10	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain B 12–1275（1264 aa） Chain B 1573–2479（907 aa） Chain B 2734–2939（206 aa） Chain B 3639–4253（615 aa） Chain B 4541–5037（497 aa） Chain E 12–1275（1264 aa） Chain E 1573–2479（907 aa） Chain E 2734–2939（206 aa） Chain E 3639–4253（615 aa） Chain E 4541–5037（497 aa） Chain G 12–1275（1264 aa） Chain G 1573–2479（907 aa） Chain G 2734–2939（206 aa） Chain G 3639–4253（615 aa） Chain G 4541–5037（497 aa） Chain I 12–1275（1264 aa） Chain I 1573–2479（907 aa） Chain I 2734–2939（206 aa） Chain I 3639–4253（615 aa） Chain I 4541–5037（497 aa）	Not recorded	ZN ZINC ION × 4 CA CALCIUM ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds	Resolution 4.60 Å
5TAZ Structure of rabbit RyR1 (ryanodine dataset, class 3) Deposited 2016-09-10	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain B 12–1275（1264 aa） Chain B 1573–2479（907 aa） Chain B 2734–2939（206 aa） Chain B 3639–4253（615 aa） Chain B 4541–5037（497 aa） Chain E 12–1275（1264 aa） Chain E 1573–2479（907 aa） Chain E 2734–2939（206 aa） Chain E 3639–4253（615 aa） Chain E 4541–5037（497 aa） Chain G 12–1275（1264 aa） Chain G 1573–2479（907 aa） Chain G 2734–2939（206 aa） Chain G 3639–4253（615 aa） Chain G 4541–5037（497 aa） Chain I 12–1275（1264 aa） Chain I 1573–2479（907 aa） Chain I 2734–2939（206 aa） Chain I 3639–4253（615 aa） Chain I 4541–5037（497 aa）	Not recorded	ZN ZINC ION × 4 CA CALCIUM ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds	Resolution 4.30 Å
5TB0 Structure of rabbit RyR1 (EGTA-only dataset, all particles) Deposited 2016-09-10	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain B 12–1275（1264 aa） Chain B 1573–2479（907 aa） Chain B 2734–2939（206 aa） Chain B 3639–4253（615 aa） Chain B 4541–5037（497 aa） Chain E 12–1275（1264 aa） Chain E 1573–2479（907 aa） Chain E 2734–2939（206 aa） Chain E 3639–4253（615 aa） Chain E 4541–5037（497 aa） Chain G 12–1275（1264 aa） Chain G 1573–2479（907 aa） Chain G 2734–2939（206 aa） Chain G 3639–4253（615 aa） Chain G 4541–5037（497 aa） Chain I 12–1275（1264 aa） Chain I 1573–2479（907 aa） Chain I 2734–2939（206 aa） Chain I 3639–4253（615 aa） Chain I 4541–5037（497 aa）	Not recorded	ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds	Resolution 4.40 Å
5TB1 Structure of rabbit RyR1 (EGTA-only dataset, class 1) Deposited 2016-09-10	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain B 12–1275（1264 aa） Chain B 1573–2479（907 aa） Chain B 2734–2939（206 aa） Chain B 3639–4253（615 aa） Chain B 4541–5037（497 aa） Chain E 12–1275（1264 aa） Chain E 1573–2479（907 aa） Chain E 2734–2939（206 aa） Chain E 3639–4253（615 aa） Chain E 4541–5037（497 aa） Chain G 12–1275（1264 aa） Chain G 1573–2479（907 aa） Chain G 2734–2939（206 aa） Chain G 3639–4253（615 aa） Chain G 4541–5037（497 aa） Chain I 12–1275（1264 aa） Chain I 1573–2479（907 aa） Chain I 2734–2939（206 aa） Chain I 3639–4253（615 aa） Chain I 4541–5037（497 aa）	Not recorded	ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds	Resolution 4.60 Å
5TB2 Structure of rabbit RyR1 (EGTA-only dataset, class 2) Deposited 2016-09-11	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain B 12–1275（1264 aa） Chain B 1573–2479（907 aa） Chain B 2734–2939（206 aa） Chain B 3639–4253（615 aa） Chain B 4541–5037（497 aa） Chain E 12–1275（1264 aa） Chain E 1573–2479（907 aa） Chain E 2734–2939（206 aa） Chain E 3639–4253（615 aa） Chain E 4541–5037（497 aa） Chain G 12–1275（1264 aa） Chain G 1573–2479（907 aa） Chain G 2734–2939（206 aa） Chain G 3639–4253（615 aa） Chain G 4541–5037（497 aa） Chain I 12–1275（1264 aa） Chain I 1573–2479（907 aa） Chain I 2734–2939（206 aa） Chain I 3639–4253（615 aa） Chain I 4541–5037（497 aa）	Not recorded	ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds	Resolution 4.60 Å
5TB3 Structure of rabbit RyR1 (EGTA-only dataset, class 3) Deposited 2016-09-11	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain B 12–1275（1264 aa） Chain B 1573–2479（907 aa） Chain B 2734–2939（206 aa） Chain B 3639–4253（615 aa） Chain B 4541–5037（497 aa） Chain E 12–1275（1264 aa） Chain E 1573–2479（907 aa） Chain E 2734–2939（206 aa） Chain E 3639–4253（615 aa） Chain E 4541–5037（497 aa） Chain G 12–1275（1264 aa） Chain G 1573–2479（907 aa） Chain G 2734–2939（206 aa） Chain G 3639–4253（615 aa） Chain G 4541–5037（497 aa） Chain I 12–1275（1264 aa） Chain I 1573–2479（907 aa） Chain I 2734–2939（206 aa） Chain I 3639–4253（615 aa） Chain I 4541–5037（497 aa）	Not recorded	ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds	Resolution 4.70 Å
5TB4 Structure of rabbit RyR1 (EGTA-only dataset, class 4) Deposited 2016-09-11	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain B 12–1275（1264 aa） Chain B 1573–2479（907 aa） Chain B 2734–2939（206 aa） Chain B 3639–4253（615 aa） Chain B 4541–5037（497 aa） Chain E 12–1275（1264 aa） Chain E 1573–2479（907 aa） Chain E 2734–2939（206 aa） Chain E 3639–4253（615 aa） Chain E 4541–5037（497 aa） Chain G 12–1275（1264 aa） Chain G 1573–2479（907 aa） Chain G 2734–2939（206 aa） Chain G 3639–4253（615 aa） Chain G 4541–5037（497 aa） Chain I 12–1275（1264 aa） Chain I 1573–2479（907 aa） Chain I 2734–2939（206 aa） Chain I 3639–4253（615 aa） Chain I 4541–5037（497 aa）	Not recorded	ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds	Resolution 4.50 Å
6FG3 Structure of Ryanodine receptor 1 in nanodiscs in the presence of calcium, ATP and ryanodine Deposited 2018-01-09	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–1275（1275 aa） Chain A 1573–2479（907 aa） Chain A 2734–2939（206 aa） Chain A 3639–5037（1399 aa） Chain B 1–1275（1275 aa） Chain B 1573–2479（907 aa） Chain B 2734–2939（206 aa） Chain B 3639–5037（1399 aa） Chain C 1–1275（1275 aa） Chain C 1573–2479（907 aa） Chain C 2734–2939（206 aa） Chain C 3639–5037（1399 aa） Chain D 1–1275（1275 aa） Chain D 1573–2479（907 aa） Chain D 2734–2939（206 aa） Chain D 3639–5037（1399 aa）	Not recorded	ZN ZINC ION × 4 CA CALCIUM ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 7.30 Å
6FOO Structure of Ryanodine Receptor 1 in nanodiscs in the presence of calcium and ATP Deposited 2018-02-08	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–1297（1297 aa） Chain A 1573–2479（907 aa） Chain A 2734–2939（206 aa） Chain A 3639–5037（1399 aa） Chain B 1–1297（1297 aa） Chain B 1573–2479（907 aa） Chain B 2734–2939（206 aa） Chain B 3639–5037（1399 aa） Chain C 1–1297（1297 aa） Chain C 1573–2479（907 aa） Chain C 2734–2939（206 aa） Chain C 3639–5037（1399 aa） Chain D 1–1297（1297 aa） Chain D 1573–2479（907 aa） Chain D 2734–2939（206 aa） Chain D 3639–5037（1399 aa）	Not recorded	ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 8.20 Å
6M2W Structure of RyR1 (Ca2+/Caffeine/ATP/CaM1234/CHL) Deposited 2020-03-01	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric	Chain A 1–2634（2634 aa） Chain A 2741–2939（199 aa） Chain A 3623–4253（631 aa） Chain A 4541–5037（497 aa） Chain D 1–2634（2634 aa） Chain D 2741–2939（199 aa） Chain D 3623–4253（631 aa） Chain D 4541–5037（497 aa） Chain G 1–2634（2634 aa） Chain G 2741–2939（199 aa） Chain G 3623–4253（631 aa） Chain G 4541–5037（497 aa） Chain J 1–2634（2634 aa） Chain J 2741–2939（199 aa） Chain J 3623–4253（631 aa） Chain J 4541–5037（497 aa）	Not recorded	CFF CAFFEINE × 4 CA CALCIUM ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 ZN ZINC ION × 4 F0U 5-bromanyl-N-[4-chloranyl-2-methyl-6-(methylcarbamoyl)phenyl]-2-(3-chloranylpyridin-2-yl)pyrazole-3-carboxamide × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.80 Å
6WOT Cryo-EM structure of recombinant rabbit Ryanodine Receptor type 1 mutant R164C in complex with FKBP12.6 Deposited 2020-04-25	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain A 1–5037（5037 aa） Chain B 1–5037（5037 aa） Chain C 1–5037（5037 aa） Chain D 1–5037（5037 aa）	Mutation：R164C Mutation：R164C Mutation：R164C Mutation：R164C	ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.54 Å
7CF9 Structure of RyR1 (Ca2+/CHL) Deposited 2020-06-24	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain A 1–2634（2634 aa） Chain A 2741–2939（199 aa） Chain A 3623–4253（631 aa） Chain A 4541–5037（497 aa） Chain C 1–2634（2634 aa） Chain C 2741–2939（199 aa） Chain C 3623–4253（631 aa） Chain C 4541–5037（497 aa） Chain E 1–2634（2634 aa） Chain E 2741–2939（199 aa） Chain E 3623–4253（631 aa） Chain E 4541–5037（497 aa） Chain G 1–2634（2634 aa） Chain G 2741–2939（199 aa） Chain G 3623–4253（631 aa） Chain G 4541–5037（497 aa）	Not recorded	CA CALCIUM ION × 4 ZN ZINC ION × 4 F0U 5-bromanyl-N-[4-chloranyl-2-methyl-6-(methylcarbamoyl)phenyl]-2-(3-chloranylpyridin-2-yl)pyrazole-3-carboxamide × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.70 Å
7M6A High resolution structure of the membrane embedded skeletal muscle ryanodine receptor Deposited 2021-03-25	Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain A 1–5037（5037 aa） Chain B 1–5037（5037 aa） Chain G 1–5037（5037 aa） Chain I 1–5037（5037 aa）	Not recorded	ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CA CALCIUM ION × 4 ZN ZINC ION × 4 CFF CAFFEINE × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.36 Å
7M6L High resolution structure of the membrane embedded skeletal muscle ryanodine receptor Deposited 2021-03-25	Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain A 1–5037（5037 aa） Chain B 1–5037（5037 aa） Chain G 1–5037（5037 aa） Chain I 1–5037（5037 aa）	Not recorded	ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CA CALCIUM ION × 4 ZN ZINC ION × 4 CFF CAFFEINE × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.98 Å
7T64 Rabbit RyR1 disease mutant Y523S in complex with FKBP12.6 embedded in lipidic nanodisc in the closed state Deposited 2021-12-13	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain A 1–5037（5037 aa） Chain B 1–5037（5037 aa） Chain C 1–5037（5037 aa） Chain D 1–5037（5037 aa）	Mutation：Y523S Mutation：Y523S Mutation：Y523S Mutation：Y523S	ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE；Blot force 2; blot for 1.0s before plunging	Resolution 4.00 Å
7T65 Rabbit RyR1 disease mutant Y523S in complex with FKBP12.6 embedded in lipidic nanodisc in the open state Deposited 2021-12-13	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain A 1–5037（5037 aa） Chain B 1–5037（5037 aa） Chain C 1–5037（5037 aa） Chain D 1–5037（5037 aa）	Mutation：Y523S Mutation：Y523S Mutation：Y523S Mutation：Y523S	ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CA CALCIUM ION × 4 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE；Blot force 2; blot for 1.0s before plunging	Resolution 4.05 Å
7TDG Rabbit RyR1 with AMP-PCP and high Ca2+ embedded in nanodisc in inactivated conformation (Dataset-A) Deposited 2021-12-31	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–1275（1275 aa） Chain A 1565–2486（922 aa） Chain A 2704–2941（238 aa） Chain A 3614–5037（1424 aa） Chain B 1–1275（1275 aa） Chain B 1565–2486（922 aa） Chain B 2704–2941（238 aa） Chain B 3614–5037（1424 aa） Chain C 1–1275（1275 aa） Chain C 1565–2486（922 aa） Chain C 2704–2941（238 aa） Chain C 3614–5037（1424 aa） Chain D 1–1275（1275 aa） Chain D 1565–2486（922 aa） Chain D 2704–2941（238 aa） Chain D 3614–5037（1424 aa）	Not recorded	ZN ZINC ION × 4 CA CALCIUM ION × 8 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 8	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE；Sample was blotted for 1 second on both sides with Whatman hardened ashless filter paper with blot force 2.	Resolution 3.80 Å
7TDH Rabbit RyR1 with AMP-PCP and high Ca2+ embedded in nanodisc in open conformation Deposited 2021-12-31	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–1275（1275 aa） Chain A 1565–2486（922 aa） Chain A 2704–2941（238 aa） Chain A 3614–5037（1424 aa） Chain B 1–1275（1275 aa） Chain B 1565–2486（922 aa） Chain B 2704–2941（238 aa） Chain B 3614–5037（1424 aa） Chain C 1–1275（1275 aa） Chain C 1565–2486（922 aa） Chain C 2704–2941（238 aa） Chain C 3614–5037（1424 aa） Chain D 1–1275（1275 aa） Chain D 1565–2486（922 aa） Chain D 2704–2941（238 aa） Chain D 3614–5037（1424 aa）	Not recorded	ZN ZINC ION × 4 CA CALCIUM ION × 8 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE；Sample was blotted for 1 second on both sides with Whatman hardened ashless filter paper with blot force 2.	Resolution 4.00 Å
7TDI Rabbit RyR1 with AMP-PCP and high Ca2+ embedded in nanodisc in closed-inactivated conformation class 2 (Dataset-A) Deposited 2021-12-31	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–1275（1275 aa） Chain A 1565–2486（922 aa） Chain A 2704–2941（238 aa） Chain A 3614–5037（1424 aa） Chain B 1–1275（1275 aa） Chain B 1565–2486（922 aa） Chain B 2704–2941（238 aa） Chain B 3614–5037（1424 aa） Chain C 1–1275（1275 aa） Chain C 1565–2486（922 aa） Chain C 2704–2941（238 aa） Chain C 3614–5037（1424 aa） Chain D 1–1275（1275 aa） Chain D 1565–2486（922 aa） Chain D 2704–2941（238 aa） Chain D 3614–5037（1424 aa）	Not recorded	ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 4 ZN ZINC ION × 4 CA CALCIUM ION × 8	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE；Sample was blotted for 1 second on both sides with Whatman hardened ashless filter paper with blot force 2.	Resolution 3.30 Å
7TDJ Rabbit RyR1 with AMP-PCP and high Ca2+ embedded in nanodisc in closed-inactivated conformation class 1(Dataset-A) Deposited 2021-12-31	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–1275（1275 aa） Chain A 1565–2486（922 aa） Chain A 2704–2941（238 aa） Chain A 3614–5037（1424 aa） Chain B 1–1275（1275 aa） Chain B 1565–2486（922 aa） Chain B 2704–2941（238 aa） Chain B 3614–5037（1424 aa） Chain C 1–1275（1275 aa） Chain C 1565–2486（922 aa） Chain C 2704–2941（238 aa） Chain C 3614–5037（1424 aa） Chain D 1–1275（1275 aa） Chain D 1565–2486（922 aa） Chain D 2704–2941（238 aa） Chain D 3614–5037（1424 aa）	Not recorded	ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 4 ZN ZINC ION × 4 CA CALCIUM ION × 8	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE；Sample was blotted for 1 second on both sides with Whatman hardened ashless filter paper with blot force 2.	Resolution 3.70 Å
7TDK Rabbit RyR1 with AMP-PCP and high Ca2+ embedded in nanodisc in closed-inactivated conformation class 3 (Dataset-A) Deposited 2021-12-31	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–1275（1275 aa） Chain A 1565–2486（922 aa） Chain A 2704–2941（238 aa） Chain A 3614–5037（1424 aa） Chain B 1–1275（1275 aa） Chain B 1565–2486（922 aa） Chain B 2704–2941（238 aa） Chain B 3614–5037（1424 aa） Chain C 1–1275（1275 aa） Chain C 1565–2486（922 aa） Chain C 2704–2941（238 aa） Chain C 3614–5037（1424 aa） Chain D 1–1275（1275 aa） Chain D 1565–2486（922 aa） Chain D 2704–2941（238 aa） Chain D 3614–5037（1424 aa）	Not recorded	ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 4 ZN ZINC ION × 4 CA CALCIUM ION × 8	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE；Sample was blotted for 1 second on both sides with Whatman hardened ashless filter paper with blot force 2.	Resolution 3.80 Å
7TZC A drug and ATP binding site in type 1 ryanodine receptor Deposited 2022-02-15	Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric	Chain A 1–5037（5037 aa） Chain B 1–5037（5037 aa） Chain G 1–5037（5037 aa） Chain I 1–5037（5037 aa）	Not recorded	CA CALCIUM ION × 20 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 ZN ZINC ION × 4 CFF CAFFEINE × 4 KVR 4-[(7-methoxy-2,3-dihydro-1,4-benzothiazepin-4(5H)-yl)methyl]benzoic acid × 4 L9R (2S)-3-(octadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 8	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.45 Å
8DRP Focus/local refined map in C4 of signal subtracted RyR1 particles Deposited 2022-07-21	Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–5037（5037 aa） Chain B 1–5037（5037 aa） Chain C 1–5037（5037 aa） Chain D 1–5037（5037 aa）	Not recorded	CFF CAFFEINE × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.84 Å
8DTB Focus/local refined map in C1 of signal subtracted RyR1 particles in complex with ImperaCalcin Deposited 2022-07-25	Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric	Chain A 1–5037（5037 aa） Chain D 1–5037（5037 aa） Chain G 1–5037（5037 aa） Chain J 1–5037（5037 aa）	Not recorded	ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 3	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.14 Å
8DUJ Global map in C1 of RyR1 particles in complex with ImperaCalcin Deposited 2022-07-27	Different construct Different oligomeric state Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 13 PDB declaration: tridecameric	Chain A 1–5037（5037 aa） Chain D 1–5037（5037 aa） Chain G 1–5037（5037 aa） Chain J 1–5037（5037 aa）	Not recorded	CFF CAFFEINE × 4 CA CALCIUM ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.70 Å
8DVE RyR1 in presence of IpCa-T26E phosphomimetic and activating ligands Deposited 2022-07-28	Different construct Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric	Chain A 1–5037（5037 aa） Chain D 1–5037（5037 aa） Chain G 1–5037（5037 aa） Chain J 1–5037（5037 aa）	Not recorded	CFF CAFFEINE × 4 CA CALCIUM ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.84 Å
8RRT Structure of rabbit RyR1 reconstituted into lipid liposomes in open state in complex with FKBP and Nb9657 Deposited 2024-01-23	Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: 12-meric	Chain B 11–5037（5027 aa） Chain E 11–5037（5027 aa） Chain G 11–5037（5027 aa） Chain J 11–5037（5027 aa）	Not recorded	ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 CA CALCIUM ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.60 Å
8RRV Structure of RyR1 in detergent in close state in complex with FKBP and Nb9657. Deposited 2024-01-23	Different construct Different ligand/ion Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: 12-meric	Chain B 1–5037（5037 aa） Chain E 1–5037（5037 aa） Chain G 1–5037（5037 aa） Chain J 1–5037（5037 aa）	Not recorded	ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
8RRW Structure of RyR1 in detergent in open state in complex with FKBP and Nb9657. Deposited 2024-01-23	Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: 12-meric	Chain B 11–5037（5027 aa） Chain E 11–5037（5027 aa） Chain G 11–5037（5027 aa） Chain J 11–5037（5027 aa）	Not recorded	ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 CA CALCIUM ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.20 Å
8RRX Structure of RyR1 reconstituted into lipid nanodisc in primed state in complex with Ca2+, ATP, caffeine and Nb9657 Deposited 2024-01-23	Different ligand/ion Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: 12-meric	Chain A 11–5037（5027 aa） Chain D 11–5037（5027 aa） Chain G 11–5037（5027 aa） Chain I 11–5037（5027 aa）	Not recorded	ZN ZINC ION × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 CA CALCIUM ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.10 Å
8RS0 Structure of RyR1 in detergent in primed state in complex with nanobody and FKBP Deposited 2024-01-24	Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: 12-meric	Chain B 11–5037（5027 aa） Chain E 11–5037（5027 aa） Chain G 11–5037（5027 aa） Chain J 11–5037（5027 aa）	Not recorded	ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 CA CALCIUM ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.30 Å
8SEN Cryo-EM Structure of RyR1 Deposited 2023-04-10	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric	Chain A 1–5037（5037 aa） Chain B 1–5037（5037 aa） Chain C 1–5037（5037 aa） Chain D 1–5037（5037 aa）	Not recorded	ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.49 Å
8SEO Cryo-EM Structure of RyR1 + ATP-gamma-S Deposited 2023-04-10	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric	Chain A 1–5037（5037 aa） Chain B 1–5037（5037 aa） Chain C 1–5037（5037 aa） Chain D 1–5037（5037 aa）	Not recorded	AGS PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER × 4 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.92 Å
8SEP Cryo-EM Structure of RyR1 + ADP Deposited 2023-04-10	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric	Chain A 1–5037（5037 aa） Chain B 1–5037（5037 aa） Chain C 1–5037（5037 aa） Chain D 1–5037（5037 aa）	Not recorded	ADP ADENOSINE-5'-DIPHOSPHATE × 4 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.57 Å
8SEQ Cryo-EM Structure of RyR1 + AMP Deposited 2023-04-10	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric	Chain A 1–5037（5037 aa） Chain B 1–5037（5037 aa） Chain C 1–5037（5037 aa） Chain D 1–5037（5037 aa）	Not recorded	AMP ADENOSINE MONOPHOSPHATE × 4 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.40 Å
8SER Cryo-EM Structure of RyR1 + Adenosine Deposited 2023-04-10	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric	Chain A 1–5037（5037 aa） Chain B 1–5037（5037 aa） Chain C 1–5037（5037 aa） Chain D 1–5037（5037 aa）	Not recorded	ADN ADENOSINE × 4 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.42 Å
8SES Cryo-EM Structure of RyR1 + Adenine Deposited 2023-04-10	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric	Chain A 1–5037（5037 aa） Chain B 1–5037（5037 aa） Chain C 1–5037（5037 aa） Chain D 1–5037（5037 aa）	Not recorded	ZN ZINC ION × 4 ADE ADENINE × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.98 Å
8SET Cryo-EM Structure of RyR1 + cAMP Deposited 2023-04-10	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric	Chain A 1–5037（5037 aa） Chain B 1–5037（5037 aa） Chain C 1–5037（5037 aa） Chain D 1–5037（5037 aa）	Not recorded	CMP ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE × 4 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.42 Å
8SEU Cryo-EM Structure of RyR1 (Local Refinement of TMD) Deposited 2023-04-10	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–5037（5037 aa） Chain B 1–5037（5037 aa） Chain C 1–5037（5037 aa） Chain D 1–5037（5037 aa）	Not recorded	ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.00 Å
8SEV Cryo-EM Structure of RyR1 + ATP-gamma-S (Local Refinement of TMD) Deposited 2023-04-10	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–5037（5037 aa） Chain B 1–5037（5037 aa） Chain C 1–5037（5037 aa） Chain D 1–5037（5037 aa）	Not recorded	AGS PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER × 4 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.17 Å
8SEW Cryo-EM Structure of RyR1 + ADP (Local Refinement of TMD) Deposited 2023-04-10	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–5037（5037 aa） Chain B 1–5037（5037 aa） Chain C 1–5037（5037 aa） Chain D 1–5037（5037 aa）	Not recorded	ADP ADENOSINE-5'-DIPHOSPHATE × 4 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.89 Å
8SEX Cryo-EM Structure of RyR1 + AMP (Local Refinement of TMD) Deposited 2023-04-10	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–5037（5037 aa） Chain B 1–5037（5037 aa） Chain C 1–5037（5037 aa） Chain D 1–5037（5037 aa）	Not recorded	AMP ADENOSINE MONOPHOSPHATE × 4 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.84 Å
8SEY Cryo-EM Structure of RyR1 + Adenosine (Local Refinement of TMD) Deposited 2023-04-10	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–5037（5037 aa） Chain B 1–5037（5037 aa） Chain C 1–5037（5037 aa） Chain D 1–5037（5037 aa）	Not recorded	ADN ADENOSINE × 4 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.99 Å
8SEZ Cryo-EM Structure of RyR1 + Adenine (Local Refinement of TMD) Deposited 2023-04-10	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–5037（5037 aa） Chain B 1–5037（5037 aa） Chain C 1–5037（5037 aa） Chain D 1–5037（5037 aa）	Not recorded	ADE ADENINE × 4 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.54 Å
8SF0 Cryo-EM Structure of RyR1 + cAMP (Local Refinement of TMD) Deposited 2023-04-10	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–5037（5037 aa） Chain B 1–5037（5037 aa） Chain C 1–5037（5037 aa） Chain D 1–5037（5037 aa）	Not recorded	CMP ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE × 4 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.90 Å
8X48 Cryo-EM structure of Ryanodine receptor 1 (EGTA) Deposited 2023-11-15	Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–5037（5037 aa） Chain B 1–5037（5037 aa） Chain C 1–5037（5037 aa） Chain D 1–5037（5037 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.00 Å
8X49 Cryo-EM structure of Ryanodine receptor 1 (100 nM Ca2+) Deposited 2023-11-15	Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–5037（5037 aa） Chain B 1–5037（5037 aa） Chain C 1–5037（5037 aa） Chain D 1–5037（5037 aa）	Not recorded	CA CALCIUM ION × 4 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.40 Å
8X4A Cryo-EM structure of Ryanodine receptor 1 (TM helix S0,100 nM Ca2+, closed state) Deposited 2023-11-15	Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–5037（5037 aa） Chain B 1–5037（5037 aa） Chain C 1–5037（5037 aa） Chain D 1–5037（5037 aa）	Not recorded	CA CALCIUM ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.80 Å
8X4B Cryo-EM structure of Ryanodine receptor 1 (TM helix S0,100 nM Ca2+, open state) Deposited 2023-11-15	Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–5037（5037 aa） Chain B 1–5037（5037 aa） Chain C 1–5037（5037 aa） Chain D 1–5037（5037 aa）	Not recorded	CA CALCIUM ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.20 Å
8X4C Cryo-EM structure of Ryanodine receptor 1 (20 uM Ca2+, 2 mM ATP) Deposited 2023-11-15	Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–5037（5037 aa） Chain B 1–5037（5037 aa） Chain C 1–5037（5037 aa） Chain D 1–5037（5037 aa）	Not recorded	ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CA CALCIUM ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.80 Å
8X4D Cryo-EM structure of Ryanodine receptor 1 (TM helix S0,20 uM Ca2+, 2 mM ATP) Deposited 2023-11-15	Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–5037（5037 aa） Chain B 1–5037（5037 aa） Chain C 1–5037（5037 aa） Chain D 1–5037（5037 aa）	Not recorded	CA CALCIUM ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.90 Å
8X4E Cryo-EM structure of Ryanodine receptor 1 (TM helix S0, 5 mM Ca2+) Deposited 2023-11-15	Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–5037（5037 aa） Chain B 1–5037（5037 aa） Chain C 1–5037（5037 aa） Chain D 1–5037（5037 aa）	Not recorded	ZN ZINC ION × 4 CA CALCIUM ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.30 Å
8XJI Structure of chimeric RyR complex with flubendiamide Deposited 2023-12-21	Different construct Different mutation/modification Different ligand/ion Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric	Chain A 1–5037（5037 aa） Chain B 1–5037（5037 aa） Chain C 1–5037（5037 aa） Chain D 1–5037（5037 aa）	Mutation：R4563K, F4564Y,C4657I, L4792S Mutation：R4563K, F4564Y,C4657I, L4792S Mutation：R4563K, F4564Y,C4657I, L4792S Mutation：R4563K, F4564Y,C4657I, L4792S	A1LVX Flubendiamide × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.91 Å
8XKH Structure of chimeric RyR Complex with tetraniliprole Deposited 2023-12-23	Different construct Different mutation/modification Different ligand/ion Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric	Chain A 1–5037（5037 aa） Chain B 1–5037（5037 aa） Chain C 1–5037（5037 aa） Chain D 1–5037（5037 aa）	Mutation：R4563K, F4564Y,C4657I, L4792S Mutation：R4563K, F4564Y,C4657I, L4792S Mutation：R4563K, F4564Y,C4657I, L4792S Mutation：R4563K, F4564Y,C4657I, L4792S	ZN ZINC ION × 4 CA CALCIUM ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 A1LV1 tetraniliprole × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.87 Å
8XLF Structure of chimeric RyR Deposited 2023-12-25	Different construct Different mutation/modification Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric	Chain A 1–5037（5037 aa） Chain B 1–5037（5037 aa） Chain C 1–5037（5037 aa） Chain D 1–5037（5037 aa）	Mutation：R4563K, F4564Y,C4657I, L4792S Mutation：R4563K, F4564Y,C4657I, L4792S Mutation：R4563K, F4564Y,C4657I, L4792S Mutation：R4563K, F4564Y,C4657I, L4792S	CA CALCIUM ION × 4 ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.62 Å
8XLH Structure of chimeric RyR-I4657M/G4819E Deposited 2023-12-26	Different construct Different mutation/modification Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric	Chain A 1–5037（5037 aa） Chain B 1–5037（5037 aa） Chain C 1–5037（5037 aa） Chain D 1–5037（5037 aa）	Mutation：R4563K, F4564Y,C4657M, L4792S,G4819E Mutation：R4563K, F4564Y,C4657M, L4792S,G4819E Mutation：R4563K, F4564Y,C4657M, L4792S,G4819E Mutation：R4563K, F4564Y,C4657M, L4792S,G4819E	ZN ZINC ION × 4 CA CALCIUM ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.62 Å
8Y40 Structure of chimeric RyR-I4657M/G4819E complex with chlorantraniliprole Deposited 2024-01-29	Different construct Different mutation/modification Different ligand/ion Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: 12-meric	Chain A 1–5037（5037 aa） Chain B 1–5037（5037 aa） Chain C 1–5037（5037 aa） Chain D 1–5037（5037 aa）	Mutation：R4563K, F4564Y,C4657M, L4792S,G4819E Mutation：R4563K, F4564Y,C4657M, L4792S,G4819E Mutation：R4563K, F4564Y,C4657M, L4792S,G4819E Mutation：R4563K, F4564Y,C4657M, L4792S,G4819E	F0U 5-bromanyl-N-[4-chloranyl-2-methyl-6-(methylcarbamoyl)phenyl]-2-(3-chloranylpyridin-2-yl)pyrazole-3-carboxamide × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.58 Å
9CGP RyR1 disease mutant Y523S with FKBP12.6, nanodisc and inhibitor dantrolene in the absence of calcium with refined P1 domain Deposited 2024-06-30	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain A 1–5037（5037 aa） Chain B 1–5037（5037 aa） Chain C 1–5037（5037 aa） Chain D 1–5037（5037 aa）	Mutation：Y523S Mutation：Y523S Mutation：Y523S Mutation：Y523S	ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 U1C Dantrolene × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.34 Å
9CGQ Rabbit RyR1 disease mutant Y523S in complex with FKBP12.6, nanodisc and inhibitor dantrolene in the presence of activating calcium Deposited 2024-06-30	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain A 1–5037（5037 aa） Chain B 1–5037（5037 aa） Chain C 1–5037（5037 aa） Chain D 1–5037（5037 aa）	Mutation：Y523S Mutation：Y523S Mutation：Y523S Mutation：Y523S	ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CA CALCIUM ION × 4 U1C Dantrolene × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.23 Å
9E17 Structure of RyR1 in the primed state in the presence of caffeine (reprocessed/reanalyzed from EMPIAR-10997, 7TZC, EMD-26205) Deposited 2024-10-21	Different construct Different ligand/ion Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: 12-meric	Chain A 1–5037（5037 aa） Chain B 1–5037（5037 aa） Chain G 1–5037（5037 aa） Chain I 1–5037（5037 aa）	Not recorded	CA CALCIUM ION × 20 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 ZN ZINC ION × 4 CFF CAFFEINE × 4 KVR 4-[(7-methoxy-2,3-dihydro-1,4-benzothiazepin-4(5H)-yl)methyl]benzoic acid × 4 L9R (2S)-3-(octadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 8	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.45 Å
9E18 Structure of RyR1 in the primed state in the presence of pentoxifylline Deposited 2024-10-21	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain A 1–5037（5037 aa） Chain B 1–5037（5037 aa） Chain C 1–5037（5037 aa） Chain D 1–5037（5037 aa）	Not recorded	ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CA CALCIUM ION × 4 ZN ZINC ION × 4 PNX 3,7-DIMETHYL-1-(5-OXOHEXYL)-3,7-DIHYDRO-1H-PURINE-2,6-DIONE × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4；2 mM pentoxifylline was added to the final sample from a 20 mM stock solution in buffer. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.68 Å
9E19 Structure of RyR1 in the open state in the presence of pentoxifylline Deposited 2024-10-21	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain A 1–5037（5037 aa） Chain B 1–5037（5037 aa） Chain C 1–5037（5037 aa） Chain D 1–5037（5037 aa）	Not recorded	ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CA CALCIUM ION × 4 ZN ZINC ION × 4 PNX 3,7-DIMETHYL-1-(5-OXOHEXYL)-3,7-DIHYDRO-1H-PURINE-2,6-DIONE × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4；2 mM pentoxifylline was added to the final sample from a 20 mM stock solution in buffer. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.04 Å
9E1A Structure of RyR1 in the primed state in the presence of dyphylline Deposited 2024-10-21	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain A 1–5037（5037 aa） Chain B 1–5037（5037 aa） Chain C 1–5037（5037 aa） Chain D 1–5037（5037 aa）	Not recorded	ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CA CALCIUM ION × 4 ZN ZINC ION × 4 A1BD2 dyphylline × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4；2 mM dyphylline was added to the final sample from a 20 mM stock solution in buffer. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.35 Å
9E1B Structure of RyR1 in the open state in the presence of dyphylline Deposited 2024-10-21	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain A 1–5037（5037 aa） Chain B 1–5037（5037 aa） Chain C 1–5037（5037 aa） Chain D 1–5037（5037 aa）	Not recorded	ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CA CALCIUM ION × 4 ZN ZINC ION × 4 A1BD2 dyphylline × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4；2 mM dyphylline was added to the final sample from a 20 mM stock solution in buffer. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.49 Å
9E1C Structure of RyR1 in the primed state in the presence of IBMX Deposited 2024-10-21	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain A 1–5037（5037 aa） Chain B 1–5037（5037 aa） Chain C 1–5037（5037 aa） Chain D 1–5037（5037 aa）	Not recorded	ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CA CALCIUM ION × 4 ZN ZINC ION × 4 IBM 3-ISOBUTYL-1-METHYLXANTHINE × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4；2 mM IBMX was added to the final sample from a 100 mM stock solution in 100% DMSO. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.63 Å
9E1D Structure of RyR1 in the primed state in the presence of enprofylline Deposited 2024-10-21	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain A 1–5037（5037 aa） Chain B 1–5037（5037 aa） Chain C 1–5037（5037 aa） Chain D 1–5037（5037 aa）	Not recorded	ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CA CALCIUM ION × 4 ZN ZINC ION × 4 A1BD3 enprofylline × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4；2 mM enprofylline was added to the final sample from a 100 mM stock solution in 100% DMSO. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.76 Å
9E1E Structure of RyR1 in the primed state in the presence of uracil Deposited 2024-10-21	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain A 1–5037（5037 aa） Chain B 1–5037（5037 aa） Chain C 1–5037（5037 aa） Chain D 1–5037（5037 aa）	Not recorded	ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CA CALCIUM ION × 4 ZN ZINC ION × 4 URA URACIL × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4；2 mM uracil was added to the final sample from a 100 mM stock solution in 100% DMSO. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.92 Å
9E1F Structure of RyR1 in the primed state in the presence of allopurinol Deposited 2024-10-21	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain A 1–5037（5037 aa） Chain B 1–5037（5037 aa） Chain C 1–5037（5037 aa） Chain D 1–5037（5037 aa）	Not recorded	ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CA CALCIUM ION × 4 ZN ZINC ION × 4 A1BD4 allopurinol × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4；2 mM allopurinol was added to the final sample from a 100 mM stock solution in 100% DMSO. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.03 Å
9E1G Structure of RyR1 in the primed state in the presence of oxypurinol Deposited 2024-10-21	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain A 1–5037（5037 aa） Chain B 1–5037（5037 aa） Chain C 1–5037（5037 aa） Chain D 1–5037（5037 aa）	Not recorded	ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CA CALCIUM ION × 4 ZN ZINC ION × 4 141 Oxypurinol × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4；2 mM oxypurinol was added to the final sample from a 100 mM stock solution in 100% DMSO. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.17 Å
9E1H Structure of RyR1 in the primed state in the presence of oxopyricid Deposited 2024-10-21	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain A 1–5037（5037 aa） Chain B 1–5037（5037 aa） Chain C 1–5037（5037 aa） Chain D 1–5037（5037 aa）	Not recorded	ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CA CALCIUM ION × 4 ZN ZINC ION × 4 A1BD5 oxopyricid × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4；2 mM oxopyricid was added to the final sample from a 20 mM stock solution in buffer. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.26 Å
9E1I Structure of RyR1 in the open state in the presence of oxopyricid Deposited 2024-10-21	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain A 1–5037（5037 aa） Chain B 1–5037（5037 aa） Chain C 1–5037（5037 aa） Chain D 1–5037（5037 aa）	Not recorded	ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CA CALCIUM ION × 4 ZN ZINC ION × 4 A1BD5 oxopyricid × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4；2 mM oxopyricid was added to the final sample from a 20 mM stock solution in buffer. cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
9HEO Open-state RyR1 in 0.05% POPC micelles, in complex with a nanobody and FKBP Deposited 2024-11-14	Different ligand/ion Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: 12-meric	Chain A 11–5037（5027 aa） Chain C 11–5037（5027 aa） Chain G 11–5037（5027 aa） Chain J 11–5037（5027 aa）	Not recorded	ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 CA CALCIUM ION × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 72	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.40 Å
9HEQ Open-state RyR1 in 0.01% POPC micelles, in complex with a nanobody and FKBP12 Deposited 2024-11-14	Different ligand/ion Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: 12-meric	Chain A 11–5037（5027 aa） Chain C 11–5037（5027 aa） Chain G 11–5037（5027 aa） Chain J 11–5037（5027 aa）	Not recorded	POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 40 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 CA CALCIUM ION × 4 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.60 Å
9OL4 Cryo-EM Structure of Ryanodine Receptor 1: Drug Bound Open Conformation Deposited 2025-05-12	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain A 1–5037（5037 aa） Chain G 1–5037（5037 aa） Chain M 1–5037（5037 aa） Chain S 1–5037（5037 aa）	Not recorded	CFF CAFFEINE × 4 CA CALCIUM ION × 4 117 7-[2-(4-FLUORO-PHENYL)-5-ISOPROPYL-3-PHENYL-4-PHENYLCARBAMOYL-PYRROL-1-YL]- 3,5-DIHYDROXY-HEPTANOIC ACID × 12 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.37 Å
9OL5 Rabbit Ryanodine Receptor 1: Atorvastatin Bound Closed Conformation Deposited 2025-05-12	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain A 1–5037（5037 aa） Chain G 1–5037（5037 aa） Chain M 1–5037（5037 aa） Chain S 1–5037（5037 aa）	Not recorded	CA CALCIUM ION × 4 117 7-[2-(4-FLUORO-PHENYL)-5-ISOPROPYL-3-PHENYL-4-PHENYLCARBAMOYL-PYRROL-1-YL]- 3,5-DIHYDROXY-HEPTANOIC ACID × 4 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.56 Å
9OL6 Rabbit Ryanodine Receptor 1: DMSO Control Closed Conformation Deposited 2025-05-12	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain A 1–5037（5037 aa） Chain G 1–5037（5037 aa） Chain M 1–5037（5037 aa） Chain S 1–5037（5037 aa）	Not recorded	CA CALCIUM ION × 4 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.11 Å
9R8O Primed-state RyR1 in 0.05% POPC micelles, in complex with a nanobody and FKBP Deposited 2025-05-16	Different ligand/ion Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: 12-meric	Chain C 11–5037（5027 aa） Chain F 11–5037（5027 aa） Chain I 11–5037（5027 aa） Chain J 11–5037（5027 aa）	Not recorded	ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 CA CALCIUM ION × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 40 ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.30 Å
9RCW Primed-state RyR1 in 0.01% POPC micelles, in complex with a nanobody and FKBP12 Deposited 2025-05-30	Different ligand/ion Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: 12-meric	Chain A 11–5037（5027 aa） Chain C 11–5037（5027 aa） Chain G 11–5037（5027 aa） Chain J 11–5037（5027 aa）	Not recorded	ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 CA CALCIUM ION × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 24	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.30 Å

124 other PDB entries and 135 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	RYR1_RABIT
Isoform	—
PDB entities	2
Chains and sequence ranges	Author chain B； PDBConstruct 1–5027； UniProt 11–5037 Author chain E； PDBConstruct 1–5027； UniProt 11–5037 Author chain G； PDBConstruct 1–5027； UniProt 11–5037 Author chain J； PDBConstruct 1–5027； UniProt 11–5037

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

P(r) Distance Distribution P(r) Distribution

Download Download SAXS (.dat) P(r) (.pr.out) CIF

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	8rru
Deposition date deposition_date	2024-01-23
Structure title title	Structure of RyR1 reconstituted into lipid liposomes in primed state in complex with FKBP and Nb9657.
Keywords keywords	Ion channel, Ca2+, tetramer, TRANSPORT PROTEIN; TRANSPORT PROTEIN
Experimental Method method	ELECTRON MICROSCOPY

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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier	— Å
Radius of gyration Rg (electron density) rg_electron	110.00 Å
Forward intensity I(0) i0	56936500000.00
Molecular weight molecular_weight	2046100.0 kDa
Excluded volume excluded_volume	2561000 Å³
Envelope volume envelope_volume	5012900 Å³
Hydration-shell volume shell_volume	380130 Å³
Envelope diameter envelope_diameter	389.7 Å
Shell Rg shell_rg	106.40 Å
Envelope Rg envelope_rg	105.40 Å
Shape Rg shape_rg	110.10 Å
Total Rg total_rg	109.90 Å
Total atoms total_atoms	143883
Residues n_residues	18208
Spherical-harmonic order n_harmonics	20
q range q_range	— – 0.5000 Å⁻¹
Data points n_points	101
Shell type shell_type	directional
Solvent electron density solvent_density	0.3340 e/Å³
Shell contrast contrast_shell	0.0300 e/Å³
CRYSOL version crysol_version	4.1.3

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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax	326.7 Å
Rg (real space) rg_real	109.90 Å
Rg uncertainty (real space) rg_real_error	1.32 Å
I(0) (real space) i0_real	5.5170e+10
I(0) uncertainty (real space) i0_real_error	1.0960e+09
Rg (reciprocal space) rg_reciprocal	109.90 Å
I(0) (reciprocal space) i0_reciprocal	56810000000.0000
Solution quality estimate total_estimate	0.9016
Solution quality rating solution_quality	EXCELLENT a EXCELLENT solution
P(r) peaks n_peaks	1
Primary peak position r_peak_primary	140.0 Å
Skewness Skewness skewness	0.240
Kurtosis Kurtosis kurtosis	-0.522
Angular range angular_range	— – 0.0700 Å⁻¹
Current regularization parameter α current_alpha	1.9620
Highest regularization parameter α highest_alpha	7239000000.0000
Real-space data points n_real_points	15
GNOM version gnom_version	4.1.3
Quality Criteria quality_criteria	AN1: 0.000; Oscil: 0.993; Stabil: 0.920; Sysdev: 1.000; Positv: 1.000; Valcen: 0.994; Smooth: 0.000

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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (7)

Entity 1 Entity 1 polymer

Description description	Peptidyl-prolyl cis-trans isomerase FKBP1B
Molecular weight formula_weight	11667.3 kDa
EC 编号 ec_number	5.2.1.8
Source method src_method	nat
Copy count entity_count	4

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A,D,H,I
Atom count atom_count	143907

GVEIETISPGDGRTFPKKGQTCVVHYTGMLQNGKKFDSSRDRNKPFKFRIGKQEVIKGFEEGAAQMSLGQRAKLTCTPDVAYGATGHPGVIPPNATLIFDVELLNLE

Entity 2 Entity 2 polymer

Description description	Ryanodine receptor 1
Molecular weight formula_weight	564931.8 kDa
Source method src_method	nat
Copy count entity_count	4

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	B,E,G,J

VQFLRTDDEVVLQCSATVLKEQLKLCLAAEGFGNRLCFLEPTSNAQNVPPDLAICCFTLEQSLSVRALQEMLANTVEAGVESSQGGGHRTLLYGHAILLRHAHSRMYLSCLTTSRSMTDKLAFDVGLQEDATGEACWWTMHPASKQRSEGEKVRVGDDLILVSVSSERYLHLSTASGELQVDASFMQTLWNMNPICSCCEEGYVTGGHVLRLFHGHMDECLTISAADSDDQRRLVYYEGGAVCTHARSLWRLEPLRISWSGSHLRWGQPLRIRHVTTGRYLALTEDQGLVVVDACKAHTKATSFCFRVSKEKLDTAPKRDVEGMGPPEIKYGESLCFVQHVASGLWLTYAAPDPKALRLGVLKKKAILHQEGHMDDALFLTRCQQEESQAARMIHSTAGLYNQFIKGLDSFSGKPRGSGPPAGPALPIEAVILSLQDLIGYFEPPSEELQHEEKQSKLRSLRNRQSLFQEEGMLSLVLNCIDRLNVYTTAAHFAEYAGEEAAESWKEIVNLLYELLASLIRGNRANCALFSTNLDWVVSKLDRLEASSGILEVLYCVLIESPEVLNIIQENHIKSIISLLDKHGRNHKVLDVLCSLCVCNGVAVRSNQDLITENLLPGRELLLQTNLINYVTSIRPNIFVGRAEGSTQYGKWYFEVMVDEVVPFLTAQATHLRVGWALTEGYSPYPGGGEGWGGNGVGDDLYSYGFDGLHLWTGHVARPVTSPGQHLLAPEDVVSCCLDLSVPSISFRINGCPVQGVFEAFNLDGLFFPVVSFSAGVKVRFLLGGRHGEFKFLPPPGYAPCHEAVLPRERLRLEPIKEYRREGPRGPHLVGPSRCLSHTDFVPCPVDTVQIVLPPHLERIREKLAENIHELWALTRIEQGWTYGPVRDDNKRLHPCLVNFHSLPEPERNYNLQMSGETLKTLLALGCHVGMADEKAEDNLKKTKLPKTYMMSNGYKPAPLDLSHVRLTPAQTTLVDRLAENGHNVWARDRVAQGWSYSAVQDIPARRNPRLVPYRLLDEATKRSNRDSLCQAVRTLLGYGYNIEPPDQEPSQVENQSRWDRVRIFRAEKSYTVQSGRWYFEFEAVTTGEMRVGWARPELRPDVELGADELAYVFNGHRGQRWHLGSEPFGRPWQSGDVVGCMIDLTENTIIFTLNGEVLMSDSGSETAFREIEIGDGFLPVCSLGPGQVGHLNLGQDVSSLRFFAICGLQEGFEPFAINMQRPVTTWFSKSLPQFEPVPPEHPHYEVARMDGTVDTPPCLRLAHRTWGSQNSLVEMLFLRLSLPVQFHQHFRCTAGATPLAPPGLQPPAEDEARAAEPDPDYENLRRSAGGWGEAEGGKEGTAKEGTPGGTPQPGVEAQPVRAENEKDATTEKNKKRGFLFKAKKAAMMTQPPATPALPRLPHDVVPADNRDDPEIILNTTTYYYSVRVFAGQEPSCVWVGWVTPDYHQHDMNFDLSKVRAVTVTMGDEQGNVHSSLKCSNCYMVWGGDFVSPGQQGRISHTDLVIGCLVDLATGLMTFTANGKESNTFFQVEPNTKLFPAVFVLPTHQNVIQFELGKQKNIMPLSAAMFLSERKNPAPQCPPRLEVQMLMPVSWSRMPNHFLQVETRRAGERLGWAVQCQDPLTMMALHIPEENRCMDILELSERLDLQRFHSHTLRLYRAVCALGNNRVAHALCSHVDQAQLLHALEDAHLPGPLRAGYYDLLISIHLESACRSRRSMLSEYIVPLTPETRAITLFPPGRKGGNARRHGLPGVGVTTSLRPPHHFSPPCFVAALPAAGVAEAPARLSPAIPLEALRDKALRMLGEAVRDGGQHARDPVGGSVEFQFVPVLKLVSTLLVMGIFGDEDVKQILKMIEPEVFTEEEEEEEEEEEEEEEEEEDEEEKEEDEEEEEKEDAEKEEEEAPEGEKEDLEEGLLQMKLPESVKLQMCNLLEYFCDQELQHRVESLAAFAERYVDKLQANQRSRYALLMRAFTMSAAETARRTREFRSPPQEQINMLLHFKDEADEEDCPLPEDIRQDLQDFHQDLLAHCGIQLEGEEEEPEEETSLSSRLRSLLETVRLVKKKEEKPEEELPAEEKKPQSLQELVSHMVVRWAQEDYVQSPELVRAMFSLLHRQYDGLGELLRALPRAYTISPSSVEDTMSLLECLGQIRSLLIVQMGPQEENLMIQSIGNIMNNKVFYQHPNLMRALGMHETVMEVMVNVLGGGETKEIRFPKMVTSCCRFLCYFCRISRQNQRSMFDHLSYLLENSGIGLGMQGSTPLDVAAASVIDNNELALALQEQDLEKVVSYLAGCGLQSCPMLLAKGYPDIGWNPCGGERYLDFLRFAVFVNGESVEENANVVVRLLIRKPECFGPALRGEGGSGLLAAIEEAIRISEDPARDGPGVRRDRRREHFGEEPPEENRVHLGHAIMSFYAALIDLLGRCAPEMHLIQAGKGEALRIRAILRSLVPLDDLVGIISLPLQIPTLGKDGALVQPKMSASFVPDHKASMVLFLDRVYGIENQDFLLHVLDVGFLPDMRAAASLDTATFSTTEMALALNRYLCLAVLPLITKCAPLFAGTEHRAIMVDSMLHTVYRLSRGRSLTKAQRDVIEDCLMALCRYIRPSMLQHLLRRLVFDVPILNEFAKMPLKLLTNHYERCWKYYCLPTGWANFGVTSEEELHLTRKLFWGIFDSLAHKKYDQELYRMAMPCLCAIAGALPPDYVDASYSSKAEKKATVDAEGNFDPRPVETLNVIIPEKLDSFINKFAEYTHEKWAFDKIQNNWSYGENVDEELKTHPMLRPYKTFSEKDKEIYRWPIKESLKAMIAWEWTIEKAREGEEERTEKKKTRKISQTAQTYDPREGYNPQPPDLSGVTLSRELQAMAEQLAENYHNTWGRKKKQELEAKGGGTHPLLVPYDTLTAKEKARDREKAQELLKFLQMNGYAVTRGLKDMELDTSSIEKRFAFGFLQQLLRWMDISQEFIAHLEAVVSSGRVEKSPHEQEIKFFAKILLPLINQYFTNHCLYFLSTPAKVLGSGGHASNKEKEMITSLFCKLAALVRHRVSLFGTDAPAVVNCLHILARSLDARTVMKSGPEIVKAGLRSFFESASEDIEKMVENLRLGKVSQARTQVKGVGQNLTYTTVALLPVLTTLFQHIAQHQFGDDVILDDVQVSCYRTLCSIYSLGTTKNTYVEKLRPALGECLARLAAAMPVAFLEPQLNEYNACSVYTTKSPRERAILGLPNSVEEMCPDIPVLDRLMADIGGLAESGARYTEMPHVIEITLPMLCSYLPRWWERGPEAPPPALPAGAPPPCTAVTSDHLNSLLGNILRIIVNNLGIDEATWMKRLAVFAQPIVSRARPELLHSHFIPTIGRLRKRAGKVVAEEEQLRLEAKAEAEEGELLVRDEFSVLCRDLYALYPLLIRYVDNNRAHWLTEPNANAEELFRMVGEIFIYWSKSHNFKREEQNFVVQNEINNMSFLTADSKSKMAKAGDAQSGGSDQERTKKKRRGDRYSVQTSLIVATLKKMLPIGLNMCAPTDQDLIMLAKTRYALKDTDEEVREFLQNNLHLQGKVEGSPSLRWQMALYRGLPGREEDADDPEKIVRRVQEVSAVLYHLEQTEHPYKSKKAVWHKLLSKQRRRAVVACFRMTPLYNLPTHRACNMFLESYKAAWILTEDHSFEDRMIDDLSKAGEQEEEEEEVEEKKPDPLHQLVLHFSRTALTEKSKLDEDYLYMAYADIMAKSCHLEEGGENGEAEEEEVEVSFEEKEMEKQRLLYQQSRLHTRGAAEMVLQMISACKGETGAMVSSTLKLGISILNGGNAEVQQKMLDYLKDKKEVGFFQSIQALMQTCSVLDLNAFERQNKAEGLGMVNEDGTVINRQNGEKVMADDEFTQDLFRFLQLLCEGHNNDFQNYLRTQTGNTTTINIIICTVDYLLRLQESISDFYWYYSGKDVIEEQGKRNFSKAMSVAKQVFNSLTEYIQGPCTGNQQSLAHSRLWDAVVGFLHVFAHMMMKLAQDSSQIELLKELLDLQKDMVVMLLSLLEGNVVNGMIARQMVDMLVESSSNVEMILKFFDMFLKLKDIVGSEAFQDYVTDPRGLISKKDFQKAMDSQKQFTGPEIQFLLSCSEADENEMINFEEFANRFQEPARDIGFNVAVLLTNLSEHVPHDPRLRNFLELAESILEYFRPYLGRIEIMGASRRIERIYFEISETNRAQWEMPQVKESKRQFIFDVVNEGGEAEKMELFVSFCEDTIFEMQIAAQISEPEGEPEADEDEGMGEAAAEGAEEGAAGAEGAAGTVAAGATARLAAAAARALRGLSYRSLRRRVRRLRRLTAREAATALAALLWAVVARAGAAGAGAAAGALRLLWGSLFGGGLVEGAKKVTVTELLAGMPDPTSDEVHGEQPAGPGGDADGAGEGEGEGDAAEGDGDEEVAGHEAGPGGAEGVVAVADGGPFRPEGAGGLGDMGDTTPAEPPTPEGSPILKRKLGVDGEEEELVPEPEPEPEPEPEKADEENGEKEEVPEAPPEPPKKAPPSPPAKKEEAGGAGMEFWGELEVQRVKFLNYLSRNFYTLRFLALFLAFAINFILLFYKVSDSPPGEDDMEGSAAGDLAGAGSGGGSGWGSGAGEEAEGDEDENMVYYFLEESTGYMEPALWCLSLLHTLVAFLCIIGYNCLKVPLVIFKREKELARKLEFDGLYITEQPGDDDVKGQWDRLVLNTPSFPSNYWDKFVKRKVLDKHGDIFGRERIAELLGMDLASLEITAHNERKPDPPPGLLTWLMSIDVKYQIWKFGVIFTDNSFLYLGWYMVMSLLGHYNNFFFAAHLLDIAMGVKTLRTILSSVTHNGKQLVMTVGLLAVVVYLYTVVAFNFFRKFYNKSEDEDEPDMKCDDMMTCYLFHMYVGVRAGGGIGDEIEDPAGDEYELYRVVFDITFFFFVIVILLAIIQGLIIDAFGELRDQQEQVKEDMETKCFICGIGSDYFDTTPHGFETHTLEEHNLANYMFFLMYLINKDETEHTGQESYVWKMYQERCWDFFPAGDCFRKQYEDQLS

Entity 3 Entity 3 polymer

Description description	Nanobody 9657
Molecular weight formula_weight	15125.5 kDa
Source method src_method	man
Copy count entity_count	4

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	C,F,K,M

QVQLQESGGGLMQAGGSLRLSCTASGSIFSINSMGWYRQAPGKQRELVATITSGNSINYADSVKGRFTISRDNAKNTVYLQMNSLKPEDTAVYYCNADRVPNGYNPWGTPNDEYDYWGQGTQVTVSSHHHHHHEPEA

来源 Source

来源生物 organism	Vicugna pacos
NCBI Class编号 ncbi_taxonomy_id	30538
宿主生物 host_organism	Escherichia coli

Entity 4 Entity 4 non-polymer

Description description	ZINC ION
Molecular weight formula_weight	65.4 kDa
Source method src_method	syn
Copy count entity_count	4

Entity 5 Entity 5 non-polymer

Description description	ADENOSINE-5'-TRIPHOSPHATE
Molecular weight formula_weight	507.2 kDa
Source method src_method	syn
Copy count entity_count	4

Entity 6 Entity 6 non-polymer

Description description	CAFFEINE
Molecular weight formula_weight	194.2 kDa
Source method src_method	syn
Copy count entity_count	4

Entity 7 Entity 7 non-polymer

Description description	CALCIUM ION
Molecular weight formula_weight	40.1 kDa
Source method src_method	syn
Copy count entity_count	4

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8. Citations (1)

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Unit-cell length a length_a	1.000 Å
Unit-cell length b length_b	1.000 Å
Unit-cell length c length_c	1.000 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	—

Space group space_group	P 1
Space-group number int_tables_number	1
Crystal system crystal_system	—

Resolution resolution	4.70 Å
Resolution测定方法 resolution_method	—
颗粒数 num_particles	16530

fit	/app/data/saxs_computation/rr/8rru/8rru.fit
pddf	/app/data/pr_computation/rr/8rru/8rru.pr.out
profile	/app/data/saxs_computation/rr/8rru/8rru.dat

Structure of RyR1 reconstituted into lipid liposomes in primed state in complex with FKBP and Nb9657.

1. Protein Identity and Related Structures Protein Identity & Related Structures

Peptidyl-prolyl cis-trans isomerase FKBP1B

State in the Current Structure

Other States of the Same Protein in the Database

Ryanodine receptor 1

State in the Current Structure

Other States of the Same Protein in the Database

SAXS scattering curve SAXS Profile

P(r) Distance Distribution P(r) Distribution

2. Structure Basics 2. Structure Basics

3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

5. Crystallography and Experiment 5. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

冷冻电镜 EM 3D

6. Entities and Polymers Entities & Polymers (7)

Entity 1 Entity 1 polymer

Polymer Polymer

Entity 2 Entity 2 polymer

Polymer Polymer

Entity 3 Entity 3 polymer

Polymer Polymer

来源 Source

Entity 4 Entity 4 non-polymer

Entity 5 Entity 5 non-polymer

Entity 6 Entity 6 non-polymer

Entity 7 Entity 7 non-polymer

8. Citations (1)

9. Files and Curves (10)

Files (7)

Curves (3)