Current Protein Identity：P00573
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1ARO T7 RNA POLYMERASE COMPLEXED WITH T7 LYSOZYME Deposited 1997-08-08 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain P
1–883（883 aa）
|
Mutation：C347S, C723S, C839S | HG MERCURY (II) ION × 7 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7；pH 7.0
|
Resolution 2.80 Å R-free 0.309 |
| 1CEZ CRYSTAL STRUCTURE OF A T7 RNA POLYMERASE-T7 PROMOTER COMPLEX Deposited 1999-03-11 | Assembly 1 Protein–DNA Monomer；Protein × 1 PDB declaration: trimeric（3） Consistent with all polymers |
Chain A
1–883（883 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 8.7；pH 8.7
|
Resolution 2.40 Å R-free 0.270 |
| 1H38 Structure of a T7 RNA polymerase elongation complex at 2.9A resolution Deposited 2002-08-24 | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric（4） Consistent with all polymers |
Chain A
1–883（883 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 8.1；10% PEG 8000, 8% GLYCEROL, 5MM BETA-MERCAPTOETHANOL,100 MM TRIS BUFFER, PH8.1, pH 8.10
|
Resolution 2.90 Å R-free 0.284 |
| 1H38 Structure of a T7 RNA polymerase elongation complex at 2.9A resolution Deposited 2002-08-24 | Assembly 2 Other combination Monomer；Protein × 1 PDB declaration: tetrameric（4） Consistent with all polymers |
Chain B
1–883（883 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 8.1；10% PEG 8000, 8% GLYCEROL, 5MM BETA-MERCAPTOETHANOL,100 MM TRIS BUFFER, PH8.1, pH 8.10
|
Resolution 2.90 Å R-free 0.284 |
| 1H38 Structure of a T7 RNA polymerase elongation complex at 2.9A resolution Deposited 2002-08-24 | Assembly 3 Other combination Monomer；Protein × 1 PDB declaration: tetrameric（4） Consistent with all polymers |
Chain C
1–883（883 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 8.1；10% PEG 8000, 8% GLYCEROL, 5MM BETA-MERCAPTOETHANOL,100 MM TRIS BUFFER, PH8.1, pH 8.10
|
Resolution 2.90 Å R-free 0.284 |
| 1H38 Structure of a T7 RNA polymerase elongation complex at 2.9A resolution Deposited 2002-08-24 | Assembly 4 Other combination Monomer；Protein × 1 PDB declaration: tetrameric（4） Consistent with all polymers |
Chain D
1–883（883 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 8.1；10% PEG 8000, 8% GLYCEROL, 5MM BETA-MERCAPTOETHANOL,100 MM TRIS BUFFER, PH8.1, pH 8.10
|
Resolution 2.90 Å R-free 0.284 |
| 1MSW Structural basis for the transition from initiation to elongation transcription in T7 RNA polymerase Deposited 2002-09-19 | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric（4） Consistent with all polymers |
Chain D
1–883（883 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；294 K；PEG 8000, LiSO4, Mg(OAc)2, Tris-HCL, pH 7.0, VAPOR DIFFUSION, HANGING DROP at 294K
|
Resolution 2.10 Å R-free 0.275 |
| 1S0V Structural basis for substrate selection by T7 RNA polymerase Deposited 2004-01-05 | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric（4） Consistent with all polymers |
Chain A
1–882（882 aa）
|
Not recorded | MG MAGNESIUM ION × 2 APC DIPHOSPHOMETHYLPHOSPHONIC ACID ADENOSYL ESTER × 1 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 3.20 Å R-free 0.307 |
| 1S0V Structural basis for substrate selection by T7 RNA polymerase Deposited 2004-01-05 | Assembly 2 Other combination Monomer；Protein × 1 PDB declaration: tetrameric（4） Consistent with all polymers |
Chain B
1–882（882 aa）
|
Not recorded | MG MAGNESIUM ION × 2 APC DIPHOSPHOMETHYLPHOSPHONIC ACID ADENOSYL ESTER × 1 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 3.20 Å R-free 0.307 |
| 1S0V Structural basis for substrate selection by T7 RNA polymerase Deposited 2004-01-05 | Assembly 3 Other combination Monomer；Protein × 1 PDB declaration: tetrameric（4） Consistent with all polymers |
Chain C
1–882（882 aa）
|
Not recorded | MG MAGNESIUM ION × 2 APC DIPHOSPHOMETHYLPHOSPHONIC ACID ADENOSYL ESTER × 1 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 3.20 Å R-free 0.307 |
| 1S0V Structural basis for substrate selection by T7 RNA polymerase Deposited 2004-01-05 | Assembly 4 Other combination Monomer；Protein × 1 PDB declaration: tetrameric（4） Consistent with all polymers |
Chain D
1–882（882 aa）
|
Not recorded | MG MAGNESIUM ION × 2 APC DIPHOSPHOMETHYLPHOSPHONIC ACID ADENOSYL ESTER × 1 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 3.20 Å R-free 0.307 |
| 1S76 T7 RNA polymerase alpha beta methylene ATP elongation complex Deposited 2004-01-29 | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric（4） Consistent with all polymers |
Chain D
1–883（883 aa）
|
Not recorded | APC DIPHOSPHOMETHYLPHOSPHONIC ACID ADENOSYL ESTER × 1 MG MAGNESIUM ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；T7 RNAP, DNA, RNA, alpha-beta methylene ATP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.88 Å R-free 0.290 |
| 1S77 T7 RNAP product pyrophosphate elongation complex Deposited 2004-01-29 | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric（4） Consistent with all polymers |
Chain D
1–883（883 aa）
|
Not recorded | MG MAGNESIUM ION × 2 POP PYROPHOSPHATE 2- × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.2；298 K；T7RNAP, DNA,RNA, 3'deoxyATP, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.69 Å R-free 0.285 |
| 2PI4 T7RNAP complexed with a phi10 protein and initiating GTPs. Deposited 2007-04-12 | Assembly 1 Protein–DNA Monomer；Protein × 1 PDB declaration: trimeric（3） Consistent with all polymers |
Chain A
6–883（878 aa）
|
Not recorded | MG MAGNESIUM ION × 2 GH3 3'-DEOXY-GUANOSINE-5'-TRIPHOSPHATE × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；300 K；50 mM Tris-HCl, 200 mM Li2SO4, 20% PEG 8000, 5% Glycerol, 15 mM magnesium acetate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 300K
|
Resolution 2.50 Å R-free 0.297 |
| 2PI5 T7 RNA polymerase complexed with a phi10 promoter Deposited 2007-04-12 | Assembly 1 Protein–DNA Monomer；Protein × 1 PDB declaration: trimeric（3） Consistent with all polymers |
Chain A
6–883（878 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；300 K；50 mM Tris-HCl, 200 mM Li2SO4, 20% PEG 8000, 5% Glycerol, 15 mM magnesium acetate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 300K
|
Resolution 2.90 Å R-free 0.268 |
| 3E2E Crystal Structure of an Intermediate Complex of T7 RNAP and 7nt of RNA Deposited 2008-08-05 | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric（4） Consistent with all polymers |
Chain A
1–883（883 aa）
|
Mutation：P266L | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；285 K；PEG 6000, MgCl2, Tris ph8.0 at 285K, VAPOR DIFFUSION, SITTING DROP
|
Resolution 3.00 Å R-free 0.292 |
| 3E3J Crystal Structure of an Intermediate Complex of T7 RNAP and 8nt of RNA Deposited 2008-08-07 | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric（4） Consistent with all polymers |
Chain B
1–883（883 aa）
|
Mutation：P266L | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；285 K；PEG 6000, MgCl2, Tris ph8.5 at 285K, VAPOR DIFFUSION, SITTING DROP
|
Resolution 6.70 Å R-free 0.454 |
| 3E3J Crystal Structure of an Intermediate Complex of T7 RNAP and 8nt of RNA Deposited 2008-08-07 | Assembly 2 Other combination Monomer；Protein × 1 PDB declaration: tetrameric（4） Consistent with all polymers |
Chain C
1–883（883 aa）
|
Mutation：P266L | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；285 K；PEG 6000, MgCl2, Tris ph8.5 at 285K, VAPOR DIFFUSION, SITTING DROP
|
Resolution 6.70 Å R-free 0.454 |
| 4RNP BACTERIOPHAGE T7 RNA POLYMERASE, HIGH SALT CRYSTAL FORM, LOW TEMPERATURE DATA, ALPHA-CARBONS ONLY Deposited 1997-09-11 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1–883（883 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 3.00 Å |
| 4RNP BACTERIOPHAGE T7 RNA POLYMERASE, HIGH SALT CRYSTAL FORM, LOW TEMPERATURE DATA, ALPHA-CARBONS ONLY Deposited 1997-09-11 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
1–883（883 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 3.00 Å |
| 4RNP BACTERIOPHAGE T7 RNA POLYMERASE, HIGH SALT CRYSTAL FORM, LOW TEMPERATURE DATA, ALPHA-CARBONS ONLY Deposited 1997-09-11 | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain C
1–883（883 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 3.00 Å |
| 8DH0 T7 RNA polymerase elongation complex with unnatural base dDs Deposited 2022-06-24 | Assembly 1 Other combination Homooligomer；Protein × 4 PDB declaration: tetradecameric（14） Consistent with all polymers |
Chain B
1–883（883 aa）
Chain F
1–883（883 aa）
Chain J
1–883（883 aa）
Chain N
1–883（883 aa）
|
Not recorded | GOL GLYCEROL × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.1；298 K；10% PEG 8000, 8% glycerol, 5 mM B-mercaptoethanol, 100 mM Tris pH 8.1
|
Resolution 2.90 Å R-free 0.261 |
| 8DH1 T7 RNA polymerase elongation complex with unnatural base dDs-PaTP pair Deposited 2022-06-24 | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric（4） Consistent with all polymers |
Chain B
1–883（883 aa）
|
Not recorded | GOL GLYCEROL × 2 S8L 1-{5-O-[(S)-hydroxy{[(R)-hydroxy(phosphonooxy)phosphoryl]oxy}phosphoryl]-beta-D-ribofuranosyl}-1H-pyrrole-2-carbaldehyde × 1 MG MAGNESIUM ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.1；298 K；10% PEG 8000, 8% glycerol, 5 mM B-mercaptoethanol, 100 mM Tris pH 8.1
|
Resolution 2.65 Å R-free 0.272 |
| 8DH1 T7 RNA polymerase elongation complex with unnatural base dDs-PaTP pair Deposited 2022-06-24 | Assembly 2 Other combination Monomer；Protein × 1 PDB declaration: tetrameric（4） Consistent with all polymers |
Chain E
1–883（883 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.1；298 K；10% PEG 8000, 8% glycerol, 5 mM B-mercaptoethanol, 100 mM Tris pH 8.1
|
Resolution 2.65 Å R-free 0.272 |
| 8DH1 T7 RNA polymerase elongation complex with unnatural base dDs-PaTP pair Deposited 2022-06-24 | Assembly 3 Other combination Monomer；Protein × 1 PDB declaration: trimeric（3） Consistent with all polymers |
Chain I
1–883（883 aa）
|
Not recorded | GOL GLYCEROL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.1；298 K；10% PEG 8000, 8% glycerol, 5 mM B-mercaptoethanol, 100 mM Tris pH 8.1
|
Resolution 2.65 Å R-free 0.272 |
| 8DH1 T7 RNA polymerase elongation complex with unnatural base dDs-PaTP pair Deposited 2022-06-24 | Assembly 4 Other combination Monomer；Protein × 1 PDB declaration: tetrameric（4） Consistent with all polymers |
Chain L
1–883（883 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.1；298 K；10% PEG 8000, 8% glycerol, 5 mM B-mercaptoethanol, 100 mM Tris pH 8.1
|
Resolution 2.65 Å R-free 0.272 |
| 8DH2 T7 RNA polymerase elongation complex with unnatural base dDs-ATP mismatch Deposited 2022-06-24 | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric（4） Consistent with all polymers |
Chain B
1–883（883 aa）
|
Not recorded | GOL GLYCEROL × 2 3PO TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.1；298 K；10% PEG 8000, 8% glycerol, 5 mM B-mercaptoethanol, 100 mM Tris pH 8.1
|
Resolution 2.90 Å R-free 0.307 |
| 8DH2 T7 RNA polymerase elongation complex with unnatural base dDs-ATP mismatch Deposited 2022-06-24 | Assembly 2 Other combination Monomer；Protein × 1 PDB declaration: tetrameric（4） Consistent with all polymers |
Chain E
1–883（883 aa）
|
Not recorded | 3PO TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.1；298 K；10% PEG 8000, 8% glycerol, 5 mM B-mercaptoethanol, 100 mM Tris pH 8.1
|
Resolution 2.90 Å R-free 0.307 |
| 8DH2 T7 RNA polymerase elongation complex with unnatural base dDs-ATP mismatch Deposited 2022-06-24 | Assembly 3 Other combination Monomer；Protein × 1 PDB declaration: trimeric（3） Consistent with all polymers |
Chain I
1–883（883 aa）
|
Not recorded | GOL GLYCEROL × 1 3PO TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.1；298 K；10% PEG 8000, 8% glycerol, 5 mM B-mercaptoethanol, 100 mM Tris pH 8.1
|
Resolution 2.90 Å R-free 0.307 |
| 8DH2 T7 RNA polymerase elongation complex with unnatural base dDs-ATP mismatch Deposited 2022-06-24 | Assembly 4 Other combination Monomer；Protein × 1 PDB declaration: tetrameric（4） Consistent with all polymers |
Chain L
1–883（883 aa）
|
Not recorded | 3PO TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.1；298 K；10% PEG 8000, 8% glycerol, 5 mM B-mercaptoethanol, 100 mM Tris pH 8.1
|
Resolution 2.90 Å R-free 0.307 |
| 8DH3 T7 RNA polymerase elongation complex with unnatural base dPa Deposited 2022-06-24 | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric（4） Consistent with all polymers |
Chain B
1–883（883 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.1；298 K；10% PEG 8000, 8% glycerol, 5 mM B-mercaptoethanol, 100 mM Tris pH 8.1
|
Resolution 3.00 Å R-free 0.276 |
| 8DH3 T7 RNA polymerase elongation complex with unnatural base dPa Deposited 2022-06-24 | Assembly 2 Other combination Monomer；Protein × 1 PDB declaration: tetrameric（4） Consistent with all polymers |
Chain E
1–883（883 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.1；298 K；10% PEG 8000, 8% glycerol, 5 mM B-mercaptoethanol, 100 mM Tris pH 8.1
|
Resolution 3.00 Å R-free 0.276 |
| 8DH3 T7 RNA polymerase elongation complex with unnatural base dPa Deposited 2022-06-24 | Assembly 3 Other combination Monomer；Protein × 1 PDB declaration: tetrameric（4） Consistent with all polymers |
Chain I
1–883（883 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.1；298 K；10% PEG 8000, 8% glycerol, 5 mM B-mercaptoethanol, 100 mM Tris pH 8.1
|
Resolution 3.00 Å R-free 0.276 |
| 8DH3 T7 RNA polymerase elongation complex with unnatural base dPa Deposited 2022-06-24 | Assembly 4 Other combination Monomer；Protein × 1 PDB declaration: tetrameric（4） Consistent with all polymers |
Chain M
1–883（883 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.1；298 K；10% PEG 8000, 8% glycerol, 5 mM B-mercaptoethanol, 100 mM Tris pH 8.1
|
Resolution 3.00 Å R-free 0.276 |
| 8DH4 T7 RNA polymerase elongation complex with unnatural base dPa-DsTP pair Deposited 2022-06-24 | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric（4） Consistent with all polymers |
Chain B
1–883（883 aa）
|
Not recorded | S96 (7P)-3-{5-O-[(R)-hydroxy{[(S)-hydroxy(phosphonooxy)phosphoryl]oxy}phosphoryl]-beta-D-ribofuranosyl}-7-(thiophen-2-yl)-3H-imidazo[4,5-b]pyridine × 1 MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.1；298 K；10% PEG 8000, 8% glycerol, 5 mM B-mercaptoethanol, 100 mM Tris pH 8.1
|
Resolution 2.80 Å R-free 0.275 |
| 8DH4 T7 RNA polymerase elongation complex with unnatural base dPa-DsTP pair Deposited 2022-06-24 | Assembly 2 Other combination Monomer；Protein × 1 PDB declaration: tetrameric（4） Consistent with all polymers |
Chain E
1–883（883 aa）
|
Not recorded | S96 (7P)-3-{5-O-[(R)-hydroxy{[(S)-hydroxy(phosphonooxy)phosphoryl]oxy}phosphoryl]-beta-D-ribofuranosyl}-7-(thiophen-2-yl)-3H-imidazo[4,5-b]pyridine × 1 MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.1；298 K；10% PEG 8000, 8% glycerol, 5 mM B-mercaptoethanol, 100 mM Tris pH 8.1
|
Resolution 2.80 Å R-free 0.275 |
| 8DH4 T7 RNA polymerase elongation complex with unnatural base dPa-DsTP pair Deposited 2022-06-24 | Assembly 3 Other combination Monomer；Protein × 1 PDB declaration: tetrameric（4） Consistent with all polymers |
Chain I
1–883（883 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.1；298 K；10% PEG 8000, 8% glycerol, 5 mM B-mercaptoethanol, 100 mM Tris pH 8.1
|
Resolution 2.80 Å R-free 0.275 |
| 8DH4 T7 RNA polymerase elongation complex with unnatural base dPa-DsTP pair Deposited 2022-06-24 | Assembly 4 Other combination Monomer；Protein × 1 PDB declaration: tetrameric（4） Consistent with all polymers |
Chain M
1–883（883 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.1；298 K；10% PEG 8000, 8% glycerol, 5 mM B-mercaptoethanol, 100 mM Tris pH 8.1
|
Resolution 2.80 Å R-free 0.275 |
| 8DH5 T7 RNA polymerase elongation complex with unnatural base dPa-ATP mismatch Deposited 2022-06-24 | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric（4） Consistent with all polymers |
Chain B
1–883（883 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.1；298 K；10% PEG 8000, 8% glycerol, 5 mM B-mercaptoethanol, 100 mM Tris pH 8.1
|
Resolution 2.85 Å R-free 0.267 |
| 8DH5 T7 RNA polymerase elongation complex with unnatural base dPa-ATP mismatch Deposited 2022-06-24 | Assembly 2 Other combination Monomer；Protein × 1 PDB declaration: tetrameric（4） Consistent with all polymers |
Chain E
1–883（883 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.1；298 K；10% PEG 8000, 8% glycerol, 5 mM B-mercaptoethanol, 100 mM Tris pH 8.1
|
Resolution 2.85 Å R-free 0.267 |
| 8DH5 T7 RNA polymerase elongation complex with unnatural base dPa-ATP mismatch Deposited 2022-06-24 | Assembly 3 Other combination Monomer；Protein × 1 PDB declaration: tetrameric（4） Consistent with all polymers |
Chain I
1–883（883 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.1；298 K；10% PEG 8000, 8% glycerol, 5 mM B-mercaptoethanol, 100 mM Tris pH 8.1
|
Resolution 2.85 Å R-free 0.267 |
| 8DH5 T7 RNA polymerase elongation complex with unnatural base dPa-ATP mismatch Deposited 2022-06-24 | Assembly 4 Other combination Monomer；Protein × 1 PDB declaration: tetrameric（4） Consistent with all polymers |
Chain M
1–883（883 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.1；298 K；10% PEG 8000, 8% glycerol, 5 mM B-mercaptoethanol, 100 mM Tris pH 8.1
|
Resolution 2.85 Å R-free 0.267 |
| 9YXM 10-23 DNAzyme in complex with T7 RNA Polymerase Deposited 2025-10-27 | Assembly 1 Protein–DNA Monomer；Protein × 1 PDB declaration: trimeric（3） Consistent with all polymers |
Chain A
1–883（883 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY | mmCIF provides none of the parsed conditions | Resolution 4.62 Å |