Current Protein Identity：P09883
New Search
Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1BXI CRYSTAL STRUCTURE OF THE ESCHERICHIA COLI COLICIN E9 DNASE DOMAIN WITH ITS COGNATE IMMUNITY PROTEIN IM9 Deposited 1998-10-04 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
449–582（134 aa）
Fragment：DNASE DOMAIN, RESIDUES 450-581
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | NI NICKEL (II) ION × 1 PO4 PHOSPHATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 5.3；24%(W/V)PEG 4K, 0.1MM SODIUM-ACETATE, PH5.3
|
Resolution 2.05 Å R-free 0.315 |
| 1EMV CRYSTAL STRUCTURE OF COLICIN E9 DNASE DOMAIN WITH ITS COGNATE IMMUNITY PROTEIN IM9 (1.7 ANGSTROMS) Deposited 2000-03-20 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
450–582（133 aa）
Fragment：C-TERMINAL DOMAIN, DNASE DOMAIN
|
Not recorded | PO4 PHOSPHATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.3；277 K；24% PEG 4000, 0.1M sodium acetate, pH 5.3, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
Resolution 1.70 Å R-free 0.260 |
| 1FR2 CRYSTAL STRUCTURE OF THE E9 DNASE DOMAIN WITH A MUTANT IMMUNITY PROTEIN IM9(E41A) Deposited 2000-09-07 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
450–582（133 aa）
Fragment：C-TERMINAL DOMAIN, DNASE DOMAIN
|
Not recorded | PO4 PHOSPHATE ION × 1 ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.3；277 K；24% PEG 4000, 0.1M sodium acetate, , pH 5.3, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
Resolution 1.60 Å R-free 0.232 |
| 1FSJ CRYSTAL STRUCTURE OF THE E9 DNASE DOMAIN Deposited 2000-09-11 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
450–582（133 aa）
Fragment：C-TERMINAL DOMAIN OF E9 COLICIN
|
Not recorded | PO4 PHOSPHATE ION × 1 ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.4；285 K；30% (w/v) PEG 5000, 0.1M sodium-acetate, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 285K
|
Resolution 1.80 Å R-free 0.222 |
| 1FSJ CRYSTAL STRUCTURE OF THE E9 DNASE DOMAIN Deposited 2000-09-11 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain C
450–582（133 aa）
Fragment：C-TERMINAL DOMAIN OF E9 COLICIN
|
Not recorded | PO4 PHOSPHATE ION × 1 ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.4；285 K；30% (w/v) PEG 5000, 0.1M sodium-acetate, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 285K
|
Resolution 1.80 Å R-free 0.222 |
| 1FSJ CRYSTAL STRUCTURE OF THE E9 DNASE DOMAIN Deposited 2000-09-11 | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain D
450–582（133 aa）
Fragment：C-TERMINAL DOMAIN OF E9 COLICIN
|
Not recorded | PO4 PHOSPHATE ION × 1 ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.4；285 K；30% (w/v) PEG 5000, 0.1M sodium-acetate, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 285K
|
Resolution 1.80 Å R-free 0.222 |
| 1FSJ CRYSTAL STRUCTURE OF THE E9 DNASE DOMAIN Deposited 2000-09-11 | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain E
450–582（133 aa）
Fragment：C-TERMINAL DOMAIN OF E9 COLICIN
|
Not recorded | PO4 PHOSPHATE ION × 1 ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.4；285 K；30% (w/v) PEG 5000, 0.1M sodium-acetate, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 285K
|
Resolution 1.80 Å R-free 0.222 |
| 1V13 CRYSTAL STRUCTURE OF THE MUTANT HIS103ALA OF THE COLICIN E9 DNASE DOMAIN IN COMPLEX WITH ZN+2 (2.0 ANGSTROMS) Deposited 2004-04-06 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
450–582（133 aa）
Chain B
450–582（133 aa）
|
Mutation：YES Mutation：YES | ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 5.8；pH 5.80
|
Resolution 2.00 Å R-free 0.307 |
| 1V14 Crystal Structure of the Colicin E9, mutant His103Ala, in complex with Mg+2 and dsDNA (resolution 2.9A) Deposited 2004-04-06 | Assembly 1 Protein–DNA Monomer；Protein × 1 PDB declaration: trimeric（3） Consistent with all polymers |
Chain A
450–582（133 aa）
Fragment：C-TERMINAL DOMAIN, RESIDUES 450-582
|
Mutation：YES | MG MAGNESIUM ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7.5；pH 7.50
|
Resolution 2.90 Å R-free 0.306 |
| 1V14 Crystal Structure of the Colicin E9, mutant His103Ala, in complex with Mg+2 and dsDNA (resolution 2.9A) Deposited 2004-04-06 | Assembly 2 Protein–DNA Monomer；Protein × 1 PDB declaration: trimeric（3） Consistent with all polymers |
Chain B
450–582（133 aa）
Fragment：C-TERMINAL DOMAIN, RESIDUES 450-582
|
Mutation：YES | MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7.5；pH 7.50
|
Resolution 2.90 Å R-free 0.306 |
| 1V14 Crystal Structure of the Colicin E9, mutant His103Ala, in complex with Mg+2 and dsDNA (resolution 2.9A) Deposited 2004-04-06 | Assembly 3 Protein–DNA Monomer；Protein × 1 PDB declaration: trimeric（3） Consistent with all polymers |
Chain C
450–582（133 aa）
Fragment：C-TERMINAL DOMAIN, RESIDUES 450-582
|
Mutation：YES | MG MAGNESIUM ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7.5；pH 7.50
|
Resolution 2.90 Å R-free 0.306 |
| 1V14 Crystal Structure of the Colicin E9, mutant His103Ala, in complex with Mg+2 and dsDNA (resolution 2.9A) Deposited 2004-04-06 | Assembly 4 Protein–DNA Monomer；Protein × 1 PDB declaration: trimeric（3） Consistent with all polymers |
Chain D
450–582（133 aa）
Fragment：C-TERMINAL DOMAIN, RESIDUES 450-582
|
Mutation：YES | MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7.5；pH 7.50
|
Resolution 2.90 Å R-free 0.306 |
| 1V15 CRYSTAL STRUCTURE OF THE COLICIN E9, MUTANT HIS103ALA, IN COMPLEX WITH ZN+2 AND DSDNA (RESOLUTION 2.4A) Deposited 2004-04-06 | Assembly 1 Protein–DNA Monomer；Protein × 1 PDB declaration: trimeric（3） Consistent with all polymers |
Chain A
450–582（133 aa）
Fragment：C-TERMINAL DOMAIN, RESIDUES 450-582
|
Mutation：YES | ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7.5；pH 7.50
|
Resolution 2.40 Å R-free 0.329 |
| 1V15 CRYSTAL STRUCTURE OF THE COLICIN E9, MUTANT HIS103ALA, IN COMPLEX WITH ZN+2 AND DSDNA (RESOLUTION 2.4A) Deposited 2004-04-06 | Assembly 2 Protein–DNA Monomer；Protein × 1 PDB declaration: trimeric（3） Consistent with all polymers |
Chain B
450–582（133 aa）
Fragment：C-TERMINAL DOMAIN, RESIDUES 450-582
|
Mutation：YES | ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7.5；pH 7.50
|
Resolution 2.40 Å R-free 0.329 |
| 1V15 CRYSTAL STRUCTURE OF THE COLICIN E9, MUTANT HIS103ALA, IN COMPLEX WITH ZN+2 AND DSDNA (RESOLUTION 2.4A) Deposited 2004-04-06 | Assembly 3 Protein–DNA Monomer；Protein × 1 PDB declaration: trimeric（3） Consistent with all polymers |
Chain C
450–582（133 aa）
Fragment：C-TERMINAL DOMAIN, RESIDUES 450-582
|
Mutation：YES | ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7.5；pH 7.50
|
Resolution 2.40 Å R-free 0.329 |
| 1V15 CRYSTAL STRUCTURE OF THE COLICIN E9, MUTANT HIS103ALA, IN COMPLEX WITH ZN+2 AND DSDNA (RESOLUTION 2.4A) Deposited 2004-04-06 | Assembly 4 Protein–DNA Monomer；Protein × 1 PDB declaration: trimeric（3） Consistent with all polymers |
Chain D
450–582（133 aa）
Fragment：C-TERMINAL DOMAIN, RESIDUES 450-582
|
Mutation：YES | ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7.5；pH 7.50
|
Resolution 2.40 Å R-free 0.329 |
| 2GYK Crystal structure of the complex of the Colicin E9 DNase domain with a mutant immunity protein, IMME9 (D51A) Deposited 2006-05-09 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
450–582（133 aa）
Fragment：C-TERMINAL DOMAIN, DNASE DOMAIN
|
Not recorded | ZN ZINC ION × 1 PO4 PHOSPHATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.3；277 K；24% (W/V) PEG 4K, 100mM SODIUM ACETATE BUFFER pH 5.3, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
Resolution 1.60 Å R-free 0.232 |
| 2GYK Crystal structure of the complex of the Colicin E9 DNase domain with a mutant immunity protein, IMME9 (D51A) Deposited 2006-05-09 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain F
450–582（133 aa）
Fragment：C-TERMINAL DOMAIN, DNASE DOMAIN
|
Not recorded | ZN ZINC ION × 1 PO4 PHOSPHATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.3；277 K；24% (W/V) PEG 4K, 100mM SODIUM ACETATE BUFFER pH 5.3, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
Resolution 1.60 Å R-free 0.232 |
| 2GZE Crystal structure of the E9 DNase domain with a mutant immunity protein IM9 (Y55A) Deposited 2006-05-11 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
450–582（133 aa）
Fragment：Colicin E9,C-terminal Domain,DNase Domain
|
Not recorded | ZN ZINC ION × 1 PO4 PHOSPHATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.3；277 K；24% (W/V) PEG 4K, 100mM SODIUM ACETATE BUFFER pH 5.3, VAPOUR DIFFUSSION,SITTING DROP, temperature 277K, VAPOR DIFFUSION, SITTING DROP
|
Resolution 1.80 Å R-free 0.242 |
| 2GZF Crystal structure of the E9 DNase domain with a mutant immunity protein IM9 (Y54F) Deposited 2006-05-11 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
450–582（133 aa）
Fragment：Colicin E9,C-terminal Domain,DNase Domain
|
Not recorded | ZN ZINC ION × 1 PO4 PHOSPHATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOUR DIFFUSSION,SITTING DROP；pH 5.3；277 K；24% (W/V) PEG 4K, 100mM SODIUM ACETATE BUFFER pH 5.3, VAPOUR DIFFUSSION,SITTING DROP, temperature 277K
|
Resolution 1.75 Å R-free 0.222 |
| 2GZG Crystal Structure of the E9 DNase Domain with a Mutant Immunity Protein IM9 (Y55F) Deposited 2006-05-11 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
450–582（133 aa）
Fragment：COLICIN E9,C-TERMINAL DOMAIN,DNASE DOMAIN
|
Not recorded | ZN ZINC ION × 1 PO4 PHOSPHATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.3；277 K；24% (W/V) PEG 4K, 100mM SODIUM ACETATE BUFFER pH 5.3, temperature 277K, VAPOR DIFFUSION, SITTING DROP
|
Resolution 1.70 Å R-free 0.204 |
| 2GZI Crystal Structure of the E9 DNase Domain with a Mutant Immunity Protein IM9 (V34A) Deposited 2006-05-11 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
450–582（133 aa）
Fragment：COLICIN E9,C-TERMINAL DOMAIN,DNASE DOMAIN
|
Not recorded | ZN ZINC ION × 1 PO4 PHOSPHATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.3；277 K；24% (W/V) PEG 4K, 100mM SODIUM ACETATE BUFFER pH 5.3, temperature 277K, VAPOR DIFFUSION, SITTING DROP
|
Resolution 1.70 Å R-free 0.256 |
| 2GZJ Crystal Structure of the E9 DNase Domain with a Mutant Immunity Protein IM9 (D51A) Deposited 2006-05-11 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
450–582（133 aa）
Fragment：COLICIN E9,C-TERMINAL DOMAIN,DNASE DOMAIN
|
Not recorded | ZN ZINC ION × 1 PO4 PHOSPHATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.3；277 K；24% (W/V) PEG 4K, 100mM SODIUM ACETATE BUFFER pH 5.3, temperature 277K, VAPOR DIFFUSION, SITTING DROP
|
Resolution 1.60 Å R-free 0.232 |
| 2GZJ Crystal Structure of the E9 DNase Domain with a Mutant Immunity Protein IM9 (D51A) Deposited 2006-05-11 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain F
450–582（133 aa）
Fragment：COLICIN E9,C-TERMINAL DOMAIN,DNASE DOMAIN
|
Not recorded | ZN ZINC ION × 1 PO4 PHOSPHATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.3；277 K；24% (W/V) PEG 4K, 100mM SODIUM ACETATE BUFFER pH 5.3, temperature 277K, VAPOR DIFFUSION, SITTING DROP
|
Resolution 1.60 Å R-free 0.232 |
| 2IVZ Structure of TolB in complex with a peptide of the colicin E9 T- domain Deposited 2006-06-23 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain E
32–47（16 aa）
Fragment：T-DOMAIN, RESIDUES 32-47
|
Not recorded | CA CALCIUM ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7.5；24% POLYETHYLENE GLYCOL MONOMETHYL ETHER 5000, 80 MM CACL2, 100 MM HEPES, PH 7.5
|
Resolution 2.00 Å R-free 0.222 |
| 2IVZ Structure of TolB in complex with a peptide of the colicin E9 T- domain Deposited 2006-06-23 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain F
32–47（16 aa）
Fragment：T-DOMAIN, RESIDUES 32-47
|
Not recorded | CA CALCIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7.5；24% POLYETHYLENE GLYCOL MONOMETHYL ETHER 5000, 80 MM CACL2, 100 MM HEPES, PH 7.5
|
Resolution 2.00 Å R-free 0.222 |
| 2IVZ Structure of TolB in complex with a peptide of the colicin E9 T- domain Deposited 2006-06-23 | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain G
32–47（16 aa）
Fragment：T-DOMAIN, RESIDUES 32-47
|
Not recorded | CA CALCIUM ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7.5；24% POLYETHYLENE GLYCOL MONOMETHYL ETHER 5000, 80 MM CACL2, 100 MM HEPES, PH 7.5
|
Resolution 2.00 Å R-free 0.222 |
| 2IVZ Structure of TolB in complex with a peptide of the colicin E9 T- domain Deposited 2006-06-23 | Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain H
32–47（16 aa）
Fragment：T-DOMAIN, RESIDUES 32-47
|
Not recorded | CA CALCIUM ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7.5；24% POLYETHYLENE GLYCOL MONOMETHYL ETHER 5000, 80 MM CACL2, 100 MM HEPES, PH 7.5
|
Resolution 2.00 Å R-free 0.222 |
| 2K5X Chemical shift structure of COLICIN E9 DNASE domain with its cognate immunity protein IM9 Deposited 2008-07-01 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
450–582（133 aa）
Fragment：UNP residues 450-582
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 6.2；298 K
|
Resolution not provided |
| 2VLN N75A mutant of E9 DNase domain in complex with Im9 Deposited 2008-01-15 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
450–582（133 aa）
Fragment：DNASE DOMAIN, RESIDUES 450-582
|
Mutation：YES | MLA MALONIC ACID × 3 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 1.60 Å R-free 0.203 |
| 2VLO K97A mutant of E9 DNase domain in complex with Im9 Deposited 2008-01-15 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
450–582（133 aa）
Fragment：DNASE DOMAIN, RESIDUES 450-582
|
Mutation：YES | SO4 SULFATE ION × 6 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 1.80 Å R-free 0.228 |
| 2VLP R54A mutant of E9 DNase domain in complex with Im9 Deposited 2008-01-15 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
450–582（133 aa）
Fragment：DNASE DOMAIN, RESIDUES 450-582
|
Mutation：YES | MLA MALONIC ACID × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
2.9 M AMMONIUM SULPHATE AND 100 MM BIS-TRIS PH 5.5
|
Resolution 2.00 Å R-free 0.229 |
| 2VLQ F86A mutant of E9 DNase domain in complex with Im9 Deposited 2008-01-15 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
450–582（133 aa）
Fragment：DNASE DOMAIN, RESIDUES 450-582
|
Mutation：YES | MLA MALONIC ACID × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
2.9 M AMMONIUM SULPHATE AND 100 MM BIS-TRIS PH 5.5
|
Resolution 1.60 Å R-free 0.199 |
| 2WPT The crystal structure of Im2 in complex with colicin E9 DNase Deposited 2009-08-10 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
450–582（133 aa）
|
Mutation：YES | GOL GLYCEROL × 2 NO3 NITRATE ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.8；pH 6.8
|
Resolution 1.78 Å R-free 0.223 |
| 3O0E Crystal structure of OmpF in complex with colicin peptide OBS1 Deposited 2010-07-19 | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain L
2–18（17 aa）
Fragment：UNP Residues 2-18
Chain N
2–18（17 aa）
Fragment：UNP Residues 2-18
Chain P
2–18（17 aa）
Fragment：UNP Residues 2-18
|
Not recorded | BOG octyl beta-D-glucopyranoside × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；25% PEG 3350, 0.2M Li2SO4, 0.1M sodium cacodylate, pH 6.5, sitting drop vapor diffusion, temperature 293K
|
Resolution 3.01 Å R-free 0.302 |
| 3O0E Crystal structure of OmpF in complex with colicin peptide OBS1 Deposited 2010-07-19 | Assembly 2 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain M
2–18（17 aa）
Fragment：UNP Residues 2-18
Chain O
2–18（17 aa）
Fragment：UNP Residues 2-18
Chain Q
2–18（17 aa）
Fragment：UNP Residues 2-18
|
Not recorded | BOG octyl beta-D-glucopyranoside × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；25% PEG 3350, 0.2M Li2SO4, 0.1M sodium cacodylate, pH 6.5, sitting drop vapor diffusion, temperature 293K
|
Resolution 3.01 Å R-free 0.302 |
| 4JML Crystal structure of the TolB(P201C)-ColicinE9 TBE peptide(A33C) complex. Deposited 2013-03-14 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain E
32–47（16 aa）
Fragment：T-domain, Residues 32-47
|
Mutation：A33C | CA CALCIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；80mM calcium chloride,
24% PEG 5000MME, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 2.00 Å R-free 0.226 |
| 5EW5 Crystal Structure of Colicin E9 In Complex with Its Immunity Protein Im9 Deposited 2015-11-20 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–582（582 aa）
|
Mutation：Y324C, L447C, D448A, K449M | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；293 K；1.3M sodium malonate, PEG3350
|
Resolution 3.20 Å R-free 0.271 |
| 5EW5 Crystal Structure of Colicin E9 In Complex with Its Immunity Protein Im9 Deposited 2015-11-20 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
1–582（582 aa）
|
Mutation：Y324C, L447C, D448A, K449M | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；293 K；1.3M sodium malonate, PEG3350
|
Resolution 3.20 Å R-free 0.271 |
| 5EW5 Crystal Structure of Colicin E9 In Complex with Its Immunity Protein Im9 Deposited 2015-11-20 | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain C
1–582（582 aa）
|
Mutation：Y324C, L447C, D448A, K449M | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；293 K；1.3M sodium malonate, PEG3350
|
Resolution 3.20 Å R-free 0.271 |
| 5EW5 Crystal Structure of Colicin E9 In Complex with Its Immunity Protein Im9 Deposited 2015-11-20 | Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain D
1–582（582 aa）
|
Mutation：Y324C, L447C, D448A, K449M | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；293 K；1.3M sodium malonate, PEG3350
|
Resolution 3.20 Å R-free 0.271 |
| 7NST ColicinE9 partial translocation complex Deposited 2021-03-08 | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric（5） Consistent with protein count |
Chain D
1–314（314 aa）
|
Mutation：A33C | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE；3 uL of diluted translocon preparation were applied on freshly glow discharged grids coated with graphene oxide (as described in https://doi.org/10.1038/s41594-019-0355-2); after 30sec waiting grids were blotted for 8-10 using -10 force and plunge frozen in liquid ethane
|
Resolution 3.70 Å |
| 7NSU ColicinE9 intact translocation complex Deposited 2021-03-08 | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain D
1–582（582 aa）
|
Mutation：A33C | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE；3 uL of diluted translocon preparation were applied on freshly glow discharged grids coated with graphene oxide (as described in https://doi.org/10.1038/s41594-019-0355-2); after 30sec waiting grids were blotted for 8-10 using -10 force and plunge frozen in liquid ethane
|
Resolution 4.70 Å |