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Protein Structures and Oligomeric States

Enter a protein name, UniProt accession or PDB ID. Name searches ask you to confirm the protein identity before related structures are queried.

Current Protein Identity：P15690 New Search
50PDB entries
50Biological assembly
50chain/assembly records
Main Difference Dimensions in This Set
Different construct Different assembly state Different ligand/ion Different experimental conditions Different structure-quality metrics

Difference tags compare only the current result set; every original PDB and assembly record remains separate.

Related-Structure Differences

Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.

PDB Entry Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Experimental Method Experimental Conditions Structure Quality
5LC5 Structure of mammalian respiratory Complex I, class2 Deposited 2016-06-19 Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric（45） Consistent with protein count
Chain G 1–232（232 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 4.35 Å
5LDW Structure of mammalian respiratory Complex I, class1 Deposited 2016-06-28 Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric（45） Consistent with protein count
Chain G 31–232（202 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus present in a cold room, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 4.27 Å
5LDX Structure of mammalian respiratory Complex I, class3. Deposited 2016-06-28 Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric（45） Consistent with protein count
Chain G 31–232（202 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer 20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7；pH 8
cryo-EM vitrification conditions Cryogen ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
Resolution 5.60 Å
5O31 Mitochondrial complex I in the deactive state Deposited 2017-05-23 Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric（45） Consistent with protein count
Chain G 24–727（704 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.55
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.13 Å
7DGQ Activity optimized supercomplex state1 Deposited 2020-11-12 Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric（80） Consistent with protein count
Chain A 24–727（704 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 CU COPPER (II) ION × 3 HEA HEME-A × 2 MG MAGNESIUM ION × 1 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 UQ2 UBIQUINONE-2 × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.00 Å
7DGR Activity optimized supercomplex state2 Deposited 2020-11-12 Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric（80） Consistent with protein count
Chain A 24–727（704 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 3 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.60 Å
7DGS Activity optimized supercomplex state3 Deposited 2020-11-12 Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric（80） Consistent with protein count
Chain A 24–727（704 aa）
Not recorded FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 7.80 Å
7DGZ Activity optimized complex I (closed form) Deposited 2020-11-12 Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric（45） Consistent with protein count
Chain A 24–727（704 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
7DH0 Activity optimized complex I (open form) Deposited 2020-11-12 Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric（45） Consistent with protein count
Chain A 24–727（704 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
7DKF Activity optimized supercomplex state4 Deposited 2020-11-24 Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric（80） Consistent with protein count
Chain A2 24–727（704 aa）
Not recorded HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 8.30 Å
7QSD Bovine complex I in the active state at 3.1 A Deposited 2022-01-13 Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric（45） Consistent with protein count
Chain G 1–727（727 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 4 LMT DODECYL-BETA-D-MALTOSIDE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7QSK Bovine complex I in lipid nanodisc, Active-Q10 Deposited 2022-01-13 Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric（45） Consistent with protein count
Chain G 1–727（727 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.84 Å
7QSL Bovine complex I in lipid nanodisc, Active-apo Deposited 2022-01-13 Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric（45） Consistent with protein count
Chain G 1–727（727 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.76 Å
7QSM Bovine complex I in lipid nanodisc, Deactive-ligand (composite) Deposited 2022-01-13 Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric（45） Consistent with protein count
Chain G 1–727（727 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 GOL GLYCEROL × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.30 Å
7QSN Bovine complex I in lipid nanodisc, Deactive-apo Deposited 2022-01-13 Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric（45） Consistent with protein count
Chain G 1–727（727 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 2.81 Å
7QSO Bovine complex I in lipid nanodisc, State 3 (Slack) Deposited 2022-01-13 Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric（45） Consistent with protein count
Chain G 1–727（727 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 6 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 2 CDL CARDIOLIPIN × 6 U10 UBIQUINONE-10 × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；blot for 10 seconds before plunging
Resolution 3.02 Å
7R41 Bovine complex I in the presence of IM1761092, active class i (Composite map) Deposited 2022-02-08 Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric（45） Consistent with protein count
Chain G 1–727（727 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R42 Bovine complex I in the presence of IM1761092, active class ii (Composite map) Deposited 2022-02-08 Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric（45） Consistent with protein count
Chain G 1–727（727 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R43 Bovine complex I in the presence of IM1761092, active class iii (Composite map) Deposited 2022-02-08 Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric（45） Consistent with protein count
Chain G 1–727（727 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 CDL CARDIOLIPIN × 5 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R44 Bovine complex I in the presence of IM1761092, active class iv (Composite map) Deposited 2022-02-08 Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric（45） Consistent with protein count
Chain G 1–727（727 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R45 Bovine complex I in the presence of IM1761092, deactive class i (Composite map) Deposited 2022-02-08 Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric（45） Consistent with protein count
Chain G 1–727（727 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R46 Bovine complex I in the presence of IM1761092, deactive class ii (Composite map) Deposited 2022-02-08 Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric（45） Consistent with protein count
Chain G 1–727（727 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 11 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R47 Bovine complex I in the presence of IM1761092, deactive class iii (Composite map) Deposited 2022-02-08 Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric（45） Consistent with protein count
Chain G 1–727（727 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R48 Bovine complex I in the presence of IM1761092, deactive class iv (Composite map) Deposited 2022-02-08 Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric（45） Consistent with protein count
Chain G 1–727（727 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4C Bovine complex I in the presence of IM1761092, deactive class v (Composite map) Deposited 2022-02-08 Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric（45） Consistent with protein count
Chain G 1–727（727 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4D Bovine complex I in the presence of IM1761092, deactive class vi (Composite map) Deposited 2022-02-08 Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric（45） Consistent with protein count
Chain G 1–727（727 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7R4F Bovine complex I in the presence of IM1761092, slack class i (Composite map) Deposited 2022-02-08 Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric（45） Consistent with protein count
Chain G 1–727（727 aa）
Not recorded LMT DODECYL-BETA-D-MALTOSIDE × 7 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 CDL CARDIOLIPIN × 6 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7R4G Bovine complex I in the presence of IM1761092, slack class ii (Composite map) Deposited 2022-02-08 Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric（45） Consistent with protein count
Chain G 1–727（727 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 10 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.14
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8Q0A Inward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric（45） Consistent with protein count
Chain G 1–727（727 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 8 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0F Inward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric（45） Consistent with protein count
Chain G 1–727（727 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 21 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0J Inward-facing, slack proteoliposome complex I at 3.8 A. Initially purified in DDM. Deposited 2023-07-28 Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric（45） Consistent with protein count
Chain G 1–727（727 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.80 Å
8Q0M Outward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric（45） Consistent with protein count
Chain G 1–727（727 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0O Outward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. Deposited 2023-07-28 Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric（45） Consistent with protein count
Chain G 1–727（727 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.10 Å
8Q0Q Outward-facing, slack proteoliposome complex I at 3.6 A. Initially purified in DDM Deposited 2023-07-28 Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric（45） Consistent with protein count
Chain G 1–727（727 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.60 Å
8Q1P Inward-facing, open2 proteoliposome complex I at 2.9 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric（45） Consistent with protein count
Chain G 1–727（727 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q1U Inward-facing, open1 proteoliposome complex I at 3.3 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric（45） Consistent with protein count
Chain G 1–727（727 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 3.30 Å
8Q1Y Outward-facing, open2 proteoliposome complex I at 2.6 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric（45） Consistent with protein count
Chain G 1–727（727 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q25 Outward-facing, open1 proteoliposome complex I at 2.8 A, after deactivation treatment. Initially purified in LMNG. Deposited 2023-08-01 Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric（45） Consistent with protein count
Chain G 1–727（727 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.80 Å
8Q45 Inward-facing, closed proteoliposome complex I at 2.7 A. Initially purified in LMNG. Deposited 2023-08-05 Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric（45） Consistent with protein count
Chain G 1–727（727 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.70 Å
8Q46 Inward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric（45） Consistent with protein count
Chain G 1–727（727 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q47 Inward-facing, open1 proteoliposome complex I at 2.9 A. Initially purified in LMNG. Deposited 2023-08-05 Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric（45） Consistent with protein count
Chain G 1–727（727 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 26 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.90 Å
8Q48 Outward-facing, closed proteoliposome complex I at 2.5 A. Initially purified in LMNG. Deposited 2023-08-05 Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric（45） Consistent with protein count
Chain G 1–727（727 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 35 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.50 Å
8Q49 Outward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric（45） Consistent with protein count
Chain G 1–727（727 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 FVH [[(2~{R},3~{S},4~{R},5~{R})-5-[(3~{R})-3-aminocarbonylpiperidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2~{R},3~{R},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3-oxidanyl-4-phosphonooxy-oxolan-2-yl]methyl hydrogen phosphate × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
8Q4A Outward-facing, open1 proteoliposome complex I at 2.6 A. Initially purified in LMNG. Deposited 2023-08-05 Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric（45） Consistent with protein count
Chain G 1–727（727 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Sample was incubated on grid for 30 s before plunge freezing
Resolution 2.60 Å
9SMF Reduced bovine complex I in lipid nanodisc, NADH-active-Q10 Deposited 2025-09-08 Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric（45） Consistent with protein count
Chain G 1–727（727 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.51 Å
9SMG Reduced bovine complex I in lipid nanodisc, NADH-active-altQ10 Deposited 2025-09-08 Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric（45） Consistent with protein count
Chain G 1–727（727 aa）
Not recorded PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.42 Å
9SMH Reduced bovine complex I in lipid nanodisc, NADH-active-DDM Deposited 2025-09-08 Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric（45） Consistent with protein count
Chain G 1–727（727 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.61 Å
9SMI Reduced bovine complex I in lipid nanodisc, NADH-deactive Deposited 2025-09-08 Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric（45） Consistent with protein count
Chain G 1–727（727 aa）
Not recorded 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 27 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 LMT DODECYL-BETA-D-MALTOSIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 CHD CHOLIC ACID × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Mix with NADH, then blot for 10 seconds before plunging
Resolution 2.01 Å
9W2U Cryo-EM structure of complex I on the bovine heart submitochondrial particles, open Deposited 2025-07-28 Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric（45） Consistent with protein count
Chain G 29–727（699 aa）
Not recorded FME N-FORMYLMETHIONINE × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 MYR MYRISTIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å
9W2V Cryo-EM structure of complex I on the bovine heart submitochondrial particles, closed Deposited 2025-07-28 Assembly 1 Protein heterocomplex Heteromer；Protein × 45 PDB declaration: 45-meric（45） Consistent with protein count
Chain G 29–727（699 aa）
Not recorded FME N-FORMYLMETHIONINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å

This page presents structural evidence recorded in PDB/mmCIF.“No recorded non-water small molecule” does not prove that the experimental sample was ligand-free; experimental conditions belong to the PDB experiment, while ligands and copy counts are shown for each biological assembly.