Current Protein Identity：P29083
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1VD4 Solution structure of the zinc finger domain of TFIIE alpha Deposited 2004-03-18 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
113–174（62 aa）
Fragment：Zinc Finger Domain
|
Not recorded | ZN ZINC ION × 1 | SOLUTION NMR |
NMR measurement conditions
pH 6；305 K；Ionic strength (raw mmCIF value) 50mM NaCl；Pressure ambient
NMR sample composition
3-4mM TFIIE alpha Zinc finger domain, U-15N, U-15N, 13C; 20mM potassium phosphate beffer, 0.03mM ZnCl2, 5mM d-DTT | 90% H2O/10% D2O
NMR sample composition
3-4mM TFIIE alpha Zinc finger domain, U-15N, U-15N, 13C; 20mM potassium phosphate beffer, 0.03mM ZnCl2, 5mM d-DTT | 99.9% D2O
|
Resolution not provided |
| 2JTX NMR structure of the TFIIE-alpha carboxyl terminus Deposited 2007-08-08 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
336–439（104 aa）
Fragment：carboxyl terminus, residues 336-439
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 7.5；300 K；Ionic strength (raw mmCIF value) 0.2；Pressure ambient
NMR sample composition
1 mM TFIIE-alpha;, 20 mM sodium phosphate, 200 mM sodium chloride, 1 mM EDTA, 100% D2O | 100% D2O
NMR sample composition
1 mM [U-100% 15N] TFIIE-alpha;, 20 mM sodium phosphate, 200 mM sodium chloride, 1 mM EDTA, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
1 mM [U-100% 13C; U-100% 15N] TFIIE-alpha;, 20 mM sodium phosphate, 200 mM sodium chloride, 1 mM EDTA, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
1 mM [U-100% 13C; U-100% 15N] TFIIE-alpha;, 20 mM sodium phosphate, 200 mM sodium chloride, 1 mM EDTA, 100% D2O | 100% D2O
|
Resolution not provided |
| 2RNQ Solution structure of the C-terminal acidic domain of TFIIE alpha Deposited 2008-01-31 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
378–439（62 aa）
Fragment：C-terminal acidic doman, UNP residues 378-439
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 6.5；295 K；Ionic strength (raw mmCIF value) 0；Pressure ambient
NMR sample composition
1-2mM TFIIE-ALPHA, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
1-2mM TFIIE-ALPHA, 100% D2O | 100% D2O
NMR sample composition
1-2mM [U-100% 15N] TFIIE-ALPHA, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
1-2mM [U-100% 13C; U-100% 15N] TFIIE-ALPHA, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
1-2mM [U-100% 13C; U-100% 15N] TFIIE-ALPHA, 100% D2O | 100% D2O
|
Resolution not provided |
| 2RNR Solution structure of the complex between TFIIE alpha C-terminal acidic domain and TFIIH p62 PH domain Deposited 2008-01-31 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
378–439（62 aa）
Fragment：C-terminal acidic domain, UNP residues 378-439
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 6.8；305 K；Ionic strength (raw mmCIF value) 0；Pressure ambient
NMR sample composition
0.5MM [U-100% 15N] TFIIE-ALPHA, 0.5MM TFIIH P62; 20MM POTASSIUM PHOSPHATE, 5MM [U-99% 2H] DTT, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
0.5MM [U-100% 13C; U-100% 15N] TFIIE-ALPHA, 0.5MM TFIIH P62; 20MM POTASSIUM PHOSPHATE, 5MM [U-99% 2H] DTT, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
0.5MM [U-100% 13C; U-100% 15N] TFIIE-ALPHA, 0.5MM TFIIH P62; 20MM POTASSIUM PHOSPHATE, 5MM [U-99% 2H] DTT, 100% D2O | 100% D2O
NMR sample composition
0.5MM [U-100% 15N] TFIIH P62, 0.5mM TFIIE-ALPHA; 20MM POTASSIUM PHOSPHATE, 5MM [U-99% 2H] DTT, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
0.5MM [U-100% 13C; U-100% 15N] TFIIH P62, 0.5MM TFIIE-ALPHA; 20MM POTASSIUM PHOSPHATE, 5MM [U-99% 2H] DTT, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
0.5MM [U-100% 13C; U-100% 15N] TFIIH P62, 0.5MM TFIIE-ALPHA; 20MM POTASSIUM PHOSPHATE, 5MM [U-99% 2H] DTT, 100% D2O | 100% D2O
|
Resolution not provided |
| 5GPY Crystal structure of the human TFIIE complex Deposited 2016-08-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–217（217 aa）
Fragment：UNP residues 1-217
|
Not recorded | ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.9；293 K；12% PEG3350
|
Resolution 2.10 Å R-free 0.237 |
| 5IY6 Human holo-PIC in the closed state Deposited 2016-03-24 | Assembly 1 Protein–DNA Heteromer；Protein × 27 PDB declaration: 29-meric（29） Consistent with all polymers |
Chain Q
1–439（439 aa）
|
Not recorded | MG MAGNESIUM ION × 2 ZN ZINC ION × 11 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE；Blot for 4 seconds before plunging into liquid ethane (FEI VITROBOT MARK IV).
|
Resolution 7.20 Å |
| 5IY7 Human holo-PIC in the open state Deposited 2016-03-24 | Assembly 1 Protein–DNA Heteromer；Protein × 27 PDB declaration: 29-meric（29） Consistent with all polymers |
Chain Q
1–439（439 aa）
|
Not recorded | MG MAGNESIUM ION × 2 ZN ZINC ION × 11 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE；Blot for 4 seconds before plunging into liquid ethane (FEI VITROBOT MARK IV).
|
Resolution 8.60 Å |
| 5IY8 Human holo-PIC in the initial transcribing state Deposited 2016-03-24 | Assembly 1 Protein–DNA Heteromer；Protein × 27 PDB declaration: 29-meric（29） Consistent with all polymers |
Chain Q
1–439（439 aa）
|
Not recorded | MG MAGNESIUM ION × 2 ZN ZINC ION × 11 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE；Blot for 4 seconds before plunging into liquid ethane (FEI VITROBOT MARK IV).
|
Resolution 7.90 Å |
| 5IY9 Human holo-PIC in the initial transcribing state (no IIS) Deposited 2016-03-24 | Assembly 1 Other combination Heteromer；Protein × 26 PDB declaration: 29-meric（29） Consistent with all polymers |
Chain Q
1–439（439 aa）
|
Not recorded | MG MAGNESIUM ION × 2 ZN ZINC ION × 10 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE；Blot for 4 seconds before plunging into liquid ethane (FEI VITROBOT MARK IV).
|
Resolution 6.30 Å |
| 5IYA Human core-PIC in the closed state Deposited 2016-03-24 | Assembly 1 Protein–DNA Heteromer；Protein × 21 PDB declaration: 23-meric（23） Consistent with all polymers |
Chain Q
1–439（439 aa）
|
Not recorded | MG MAGNESIUM ION × 2 ZN ZINC ION × 11 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE；Blot for 4 seconds before plunging into liquid ethane (FEI VITROBOT MARK IV).
|
Resolution 5.40 Å |
| 5IYB Human core-PIC in the open state Deposited 2016-03-24 | Assembly 1 Protein–DNA Heteromer；Protein × 21 PDB declaration: 23-meric（23） Consistent with all polymers |
Chain Q
1–439（439 aa）
|
Not recorded | MG MAGNESIUM ION × 2 ZN ZINC ION × 11 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE；Blot for 4 seconds before plunging into liquid ethane (FEI VITROBOT MARK IV).
|
Resolution 3.90 Å |
| 5IYC Human core-PIC in the initial transcribing state Deposited 2016-03-24 | Assembly 1 Protein–DNA Heteromer；Protein × 21 PDB declaration: 23-meric（23） Consistent with all polymers |
Chain Q
1–439（439 aa）
|
Not recorded | MG MAGNESIUM ION × 2 ZN ZINC ION × 11 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE；Blot for 4 seconds before plunging into liquid ethane (FEI VITROBOT MARK IV).
|
Resolution 3.90 Å |
| 5IYD Human core-PIC in the initial transcribing state (no IIS) Deposited 2016-03-24 | Assembly 1 Other combination Heteromer；Protein × 20 PDB declaration: 23-meric（23） Consistent with all polymers |
Chain Q
1–439（439 aa）
|
Not recorded | MG MAGNESIUM ION × 2 ZN ZINC ION × 11 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE；Blot for 4 seconds before plunging into liquid ethane (FEI VITROBOT MARK IV).
|
Resolution 3.90 Å |
| 6O9L Human holo-PIC in the closed state Deposited 2019-03-14 | Assembly 1 Protein–DNA Heteromer；Protein × 31 PDB declaration: 33-meric（33） Consistent with all polymers |
Chain Q
1–439（439 aa）
|
Not recorded | MG MAGNESIUM ION × 2 ZN ZINC ION × 17 SF4 IRON/SULFUR CLUSTER × 1 | ELECTRON MICROSCOPY |
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 7.20 Å |
| 7EG9 TFIID-based intermediate PIC on SCP promoter Deposited 2021-03-24 | Assembly 1 Protein–DNA Heteromer；Protein × 39 PDB declaration: 41-meric（41） Consistent with all polymers |
Chain U
1–439（439 aa）
|
Not recorded | ZN ZINC ION × 10 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 7EGA TFIID-based intermediate PIC on PUMA promoter Deposited 2021-03-24 | Assembly 1 Protein–DNA Heteromer；Protein × 39 PDB declaration: 41-meric（41） Consistent with all polymers |
Chain U
1–439（439 aa）
|
Not recorded | ZN ZINC ION × 10 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.10 Å |
| 7EGB TFIID-based holo PIC on SCP promoter Deposited 2021-03-24 | Assembly 1 Protein–DNA Heteromer；Protein × 49 PDB declaration: 51-meric（51） Consistent with all polymers |
Chain U
1–439（439 aa）
|
Not recorded | ZN ZINC ION × 17 SF4 IRON/SULFUR CLUSTER × 1 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 7EGC p53-bound TFIID-based holo PIC on HDM2 promoter Deposited 2021-03-24 | Assembly 1 Protein–DNA Heteromer；Protein × 49 PDB declaration: 51-meric（51） Consistent with all polymers |
Chain U
1–439（439 aa）
|
Not recorded | ZN ZINC ION × 16 SF4 IRON/SULFUR CLUSTER × 1 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.90 Å |
| 7ENA TFIID-based PIC-Mediator holo-complex in pre-assembled state (pre-hPIC-MED) Deposited 2021-04-16 | Assembly 1 Protein–DNA Heteromer；Protein × 74 PDB declaration: 76-meric（76） Consistent with all polymers |
Chain EA
1–439（439 aa）
|
Not recorded | ZN ZINC ION × 16 MG MAGNESIUM ION × 1 SF4 IRON/SULFUR CLUSTER × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.07 Å |
| 7ENC TFIID-based PIC-Mediator holo-complex in fully-assembled state (hPIC-MED) Deposited 2021-04-16 | Assembly 1 Protein–DNA Heteromer；Protein × 74 PDB declaration: 76-meric（76） Consistent with all polymers |
Chain EA
1–439（439 aa）
|
Not recorded | ZN ZINC ION × 16 SF4 IRON/SULFUR CLUSTER × 1 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.13 Å |
| 7LBM Structure of the human Mediator-bound transcription pre-initiation complex Deposited 2021-01-08 | Assembly 1 Protein–DNA Heteromer；Protein × 54 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain Q
1–439（439 aa）
|
Not recorded | MG MAGNESIUM ION × 2 ZN ZINC ION × 16 SF4 IRON/SULFUR CLUSTER × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.80 Å |
| 7NVR Human Mediator with RNA Polymerase II Pre-initiation complex Deposited 2021-03-15 | Assembly 1 Protein–DNA Heteromer；Protein × 56 PDB declaration: 58-meric（58） Consistent with all polymers |
Chain W
1–439（439 aa）
|
Not recorded | SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 18 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.50 Å |
| 7NVS RNA polymerase II core pre-initiation complex with closed promoter DNA in proximal position Deposited 2021-03-16 | Assembly 1 Protein–DNA Heteromer；Protein × 20 PDB declaration: 22-meric（22） Consistent with all polymers |
Chain W
1–439（439 aa）
|
Not recorded | ZN ZINC ION × 10 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.80 Å |
| 7NVT RNA polymerase II core pre-initiation complex with closed promoter DNA in distal position Deposited 2021-03-16 | Assembly 1 Protein–DNA Heteromer；Protein × 20 PDB declaration: 22-meric（22） Consistent with all polymers |
Chain W
1–439（439 aa）
|
Not recorded | ZN ZINC ION × 10 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 7NVU RNA polymerase II core pre-initiation complex with open promoter DNA Deposited 2021-03-16 | Assembly 1 Protein–DNA Heteromer；Protein × 20 PDB declaration: 22-meric（22） Consistent with all polymers |
Chain W
1–439（439 aa）
|
Not recorded | ZN ZINC ION × 10 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.50 Å |
| 7NVV XPB-containing part of TFIIH in a post-translocated state (with ADP-BeF3) Deposited 2021-03-16 | Assembly 1 Protein–DNA Heteromer；Protein × 6 PDB declaration: octameric（8） Consistent with all polymers |
Chain W
1–439（439 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 BEF BERYLLIUM TRIFLUORIDE ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 7NVW TFIIH in a pre-translocated state (without ADP-BeF3) Deposited 2021-03-16 | Assembly 1 Protein–DNA Heteromer；Protein × 11 PDB declaration: tridecameric（13） Consistent with all polymers |
Chain W
1–439（439 aa）
|
Not recorded | SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 7 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.30 Å |
| 7NVX TFIIH in a post-translocated state (with ADP-BeF3) Deposited 2021-03-16 | Assembly 1 Protein–DNA Heteromer；Protein × 11 PDB declaration: tridecameric（13） Consistent with all polymers |
Chain W
1–439（439 aa）
|
Not recorded | SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 7 ADP ADENOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 BEF BERYLLIUM TRIFLUORIDE ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.90 Å |
| 7NVY RNA polymerase II pre-initiation complex with closed promoter DNA in proximal position Deposited 2021-03-16 | Assembly 1 Protein–DNA Heteromer；Protein × 30 PDB declaration: 32-meric（32） Consistent with all polymers |
Chain W
1–439（439 aa）
|
Not recorded | SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 17 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 7.30 Å |
| 7NVZ RNA polymerase II pre-initiation complex with closed promoter DNA in distal position Deposited 2021-03-16 | Assembly 1 Protein–DNA Heteromer；Protein × 30 PDB declaration: 32-meric（32） Consistent with all polymers |
Chain W
1–439（439 aa）
|
Not recorded | SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 17 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 7.20 Å |
| 7NW0 RNA polymerase II pre-initiation complex with open promoter DNA Deposited 2021-03-16 | Assembly 1 Protein–DNA Heteromer；Protein × 30 PDB declaration: 32-meric（32） Consistent with all polymers |
Chain W
1–439（439 aa）
|
Not recorded | SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 15 ADP ADENOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 2 BEF BERYLLIUM TRIFLUORIDE ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.60 Å |
| 8BVW RNA polymerase II pre-initiation complex with the distal +1 nucleosome (PIC-Nuc18W) Deposited 2022-12-20 | Assembly 1 Protein–DNA Heteromer；Protein × 40 PDB declaration: 42-meric（42） Consistent with all polymers |
Chain W
1–273（273 aa）
Chain W
274–439（166 aa）
|
Not recorded | SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 17 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.00 Å |
| 8BYQ RNA polymerase II pre-initiation complex with the proximal +1 nucleosome (PIC-Nuc10W) Deposited 2022-12-14 | Assembly 1 Protein–DNA Heteromer；Protein × 38 PDB declaration: 40-meric（40） Consistent with all polymers |
Chain W
1–196（196 aa）
|
Not recorded | SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 16 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.10 Å |
| 8GXQ PIC-Mediator in complex with +1 nucleosome (T40N) in MH-binding state Deposited 2022-09-21 | Assembly 1 Protein–DNA Heteromer；Protein × 83 PDB declaration: 87-meric（87） Consistent with all polymers |
Chain EA
1–439（439 aa）
|
Not recorded | ZN ZINC ION × 19 SF4 IRON/SULFUR CLUSTER × 1 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.04 Å |
| 8GXS PIC-Mediator in complex with +1 nucleosome (T40N) in H-binding state Deposited 2022-09-21 | Assembly 1 Protein–DNA Heteromer；Protein × 83 PDB declaration: 87-meric（87） Consistent with all polymers |
Chain EA
1–439（439 aa）
|
Not recorded | ZN ZINC ION × 19 SF4 IRON/SULFUR CLUSTER × 1 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.16 Å |
| 8S51 RNA polymerase II core initially transcribing complex with an ordered RNA of 8 nt Deposited 2024-02-22 | Assembly 1 Other combination Heteromer；Protein × 20 PDB declaration: 23-meric（23） Consistent with all polymers |
Chain W
1–439（439 aa）
|
Not recorded | ZN ZINC ION × 10 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 8S52 RNA polymerase II core initially transcribing complex with an ordered RNA of 10 nt Deposited 2024-02-22 | Assembly 1 Other combination Heteromer；Protein × 20 PDB declaration: 23-meric（23） Consistent with all polymers |
Chain W
1–439（439 aa）
|
Not recorded | ZN ZINC ION × 10 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 8S54 RNA polymerase II early elongation complex bound to TFIIE and TFIIF - state b (composite structure) Deposited 2024-02-22 | Assembly 1 Other combination Heteromer；Protein × 15 PDB declaration: 18-meric（18） Consistent with all polymers |
Chain W
1–439（439 aa）
|
Not recorded | ZN ZINC ION × 9 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 8S55 RNA polymerase II early elongation complex bound to TFIIE and TFIIF - state a (composite structure) Deposited 2024-02-22 | Assembly 1 Other combination Heteromer；Protein × 16 PDB declaration: 19-meric（19） Consistent with all polymers |
Chain W
1–439（439 aa）
|
Not recorded | ZN ZINC ION × 9 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 8S5N RNA polymerase II core initially transcribing complex with an ordered RNA of 12 nt Deposited 2024-02-24 | Assembly 1 Other combination Heteromer；Protein × 20 PDB declaration: 23-meric（23） Consistent with all polymers |
Chain W
1–439（439 aa）
|
Not recorded | ZN ZINC ION × 10 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 8WAK Structure of transcribing complex 2 (TC2), the initially transcribing complex with Pol II positioned 2nt downstream of TSS. Deposited 2023-09-07 | Assembly 1 Other combination Heteromer；Protein × 48 PDB declaration: 51-meric（51） Consistent with all polymers |
Chain U
1–439（439 aa）
|
Not recorded | ZN ZINC ION × 17 SF4 IRON/SULFUR CLUSTER × 1 W0F [[(2~{S},3~{R},4~{S},5~{R})-5-(2-azanyl-6-oxidanylidene-3~{H}-purin-9-yl)-3-methoxy-4-oxidanyl-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] phosphono hydrogen phosphate × 1 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.47 Å |
| 8WAL Structure of transcribing complex 3 (TC3), the initially transcribing complex with Pol II positioned 3nt downstream of TSS. Deposited 2023-09-07 | Assembly 1 Other combination Heteromer；Protein × 48 PDB declaration: 51-meric（51） Consistent with all polymers |
Chain U
1–439（439 aa）
|
Not recorded | ZN ZINC ION × 17 SF4 IRON/SULFUR CLUSTER × 1 W0F [[(2~{S},3~{R},4~{S},5~{R})-5-(2-azanyl-6-oxidanylidene-3~{H}-purin-9-yl)-3-methoxy-4-oxidanyl-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] phosphono hydrogen phosphate × 1 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 8.52 Å |
| 8WAN Structure of transcribing complex 4 (TC4), the initially transcribing complex with Pol II positioned 4nt downstream of TSS. Deposited 2023-09-07 | Assembly 1 Other combination Heteromer；Protein × 48 PDB declaration: 51-meric（51） Consistent with all polymers |
Chain U
1–439（439 aa）
|
Not recorded | ZN ZINC ION × 17 SF4 IRON/SULFUR CLUSTER × 1 MG MAGNESIUM ION × 1 W0F [[(2~{S},3~{R},4~{S},5~{R})-5-(2-azanyl-6-oxidanylidene-3~{H}-purin-9-yl)-3-methoxy-4-oxidanyl-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] phosphono hydrogen phosphate × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.07 Å |
| 8WAO Structure of transcribing complex 5 (TC5), the initially transcribing complex with Pol II positioned 5nt downstream of TSS. Deposited 2023-09-07 | Assembly 1 Other combination Heteromer；Protein × 48 PDB declaration: 51-meric（51） Consistent with all polymers |
Chain U
1–439（439 aa）
|
Not recorded | ZN ZINC ION × 17 SF4 IRON/SULFUR CLUSTER × 1 MG MAGNESIUM ION × 1 W0F [[(2~{S},3~{R},4~{S},5~{R})-5-(2-azanyl-6-oxidanylidene-3~{H}-purin-9-yl)-3-methoxy-4-oxidanyl-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] phosphono hydrogen phosphate × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.40 Å |
| 8WAP Structure of transcribing complex 6 (TC6), the initially transcribing complex with Pol II positioned 6nt downstream of TSS. Deposited 2023-09-08 | Assembly 1 Other combination Heteromer；Protein × 48 PDB declaration: 51-meric（51） Consistent with all polymers |
Chain U
1–439（439 aa）
|
Not recorded | ZN ZINC ION × 17 SF4 IRON/SULFUR CLUSTER × 1 MG MAGNESIUM ION × 1 W0F [[(2~{S},3~{R},4~{S},5~{R})-5-(2-azanyl-6-oxidanylidene-3~{H}-purin-9-yl)-3-methoxy-4-oxidanyl-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] phosphono hydrogen phosphate × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.85 Å |
| 8WAQ Structure of transcribing complex 7 (TC7), the initially transcribing complex with Pol II positioned 7nt downstream of TSS. Deposited 2023-09-08 | Assembly 1 Other combination Heteromer；Protein × 48 PDB declaration: 51-meric（51） Consistent with all polymers |
Chain U
1–439（439 aa）
|
Not recorded | ZN ZINC ION × 17 SF4 IRON/SULFUR CLUSTER × 1 MG MAGNESIUM ION × 1 W0F [[(2~{S},3~{R},4~{S},5~{R})-5-(2-azanyl-6-oxidanylidene-3~{H}-purin-9-yl)-3-methoxy-4-oxidanyl-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] phosphono hydrogen phosphate × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.29 Å |
| 8WAR Structure of transcribing complex 8 (TC8), the initially transcribing complex with Pol II positioned 8nt downstream of TSS. Deposited 2023-09-08 | Assembly 1 Other combination Heteromer；Protein × 48 PDB declaration: 51-meric（51） Consistent with all polymers |
Chain U
1–439（439 aa）
|
Not recorded | ZN ZINC ION × 17 SF4 IRON/SULFUR CLUSTER × 1 MG MAGNESIUM ION × 1 W0F [[(2~{S},3~{R},4~{S},5~{R})-5-(2-azanyl-6-oxidanylidene-3~{H}-purin-9-yl)-3-methoxy-4-oxidanyl-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] phosphono hydrogen phosphate × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 7.20 Å |
| 8WAS Structure of transcribing complex 9 (TC9), the initially transcribing complex with Pol II positioned 9nt downstream of TSS. Deposited 2023-09-08 | Assembly 1 Other combination Heteromer；Protein × 48 PDB declaration: 51-meric（51） Consistent with all polymers |
Chain U
1–439（439 aa）
|
Not recorded | ZN ZINC ION × 17 SF4 IRON/SULFUR CLUSTER × 1 MG MAGNESIUM ION × 1 W0F [[(2~{S},3~{R},4~{S},5~{R})-5-(2-azanyl-6-oxidanylidene-3~{H}-purin-9-yl)-3-methoxy-4-oxidanyl-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] phosphono hydrogen phosphate × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.13 Å |