Current Protein Identity：P63272
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 3H7H Crystal structure of the human transcription elongation factor DSIF, hSpt4/hSpt5 (176-273) Deposited 2009-04-27 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
2–117（116 aa）
|
Not recorded | BME BETA-MERCAPTOETHANOL × 1 ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；290 K；Ammonium sulfate, PEG 3350, Tris/HCl, beta-mercaptoethanol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K
|
Resolution 1.55 Å R-free 0.222 |
| 5OIK Structure of an RNA polymerase II-DSIF transcription elongation complex Deposited 2017-07-18 | Assembly 1 Other combination Heteromer；Protein × 14 PDB declaration: heptadecameric（17） Consistent with all polymers |
Chain Y
1–117（117 aa）
|
Not recorded | ZN ZINC ION × 8 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.25；pH was adjusted at 25 degrees Celsius
cryo-EM vitrification conditions
Cryogen ETHANE；Sample (four microliters) was applied to glow-discharged Quantifoil R 2/1 holey carbon grids, which were then blotted for 8.5s and plunge-frozen in liquid ethane.
|
Resolution 3.70 Å |
| 6GMH Structure of activated transcription complex Pol II-DSIF-PAF-SPT6 Deposited 2018-05-26 | Assembly 1 Other combination Heteromer；Protein × 20 PDB declaration: heptadecameric（17） Review required |
Chain Y
1–117（117 aa）
|
Not recorded | ZN ZINC ION × 9 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；10s wait prior to blotting, blotting 8.5s
|
Resolution 3.10 Å |
| 6GML Structure of paused transcription complex Pol II-DSIF-NELF Deposited 2018-05-27 | Assembly 1 Other combination Heteromer；Protein × 18 PDB declaration: 21-meric（21） Consistent with all polymers |
Chain Y
1–117（117 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 9 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 6TED Structure of complete, activated transcription complex Pol II-DSIF-PAF-SPT6 uncovers allosteric elongation activation by RTF1 Deposited 2019-11-11 | Assembly 1 Other combination Heteromer；Protein × 21 PDB declaration: 24-meric（24） Consistent with all polymers |
Chain Y
1–117（117 aa）
|
Not recorded | ZN ZINC ION × 9 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；2 microliters applied to both sides of grid. Sample incubated on grid for 10s prior to blotting. Blotting for 8.5s.
|
Resolution 3.10 Å |
| 7OKY Structure of active transcription elongation complex Pol II-DSIF-ELL2-EAF1(composite structure) Deposited 2021-05-18 | Assembly 1 Other combination Heteromer；Protein × 16 PDB declaration: nonadecameric（19） Consistent with all polymers |
Chain Y
1–117（117 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 9 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.14 Å |
| 7UND Pol II-DSIF-SPT6-PAF1c-TFIIS-nucleosome complex (stalled at +38) Deposited 2022-04-10 | Assembly 1 Other combination Heteromer；Protein × 30 PDB declaration: 33-meric（33） Consistent with all polymers |
Chain Y
1–117（117 aa）
|
Not recorded | ZN ZINC ION × 9 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 7YCX The structure of INTAC-PEC complex Deposited 2022-07-02 | Assembly 1 Other combination Heteromer；Protein × 31 PDB declaration: 34-meric（34） Consistent with all polymers |
Chain i
1–117（117 aa）
|
Not recorded | ZN ZINC ION × 10 MN MANGANESE (II) ION × 2 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.18 Å |
| 8P4C Structural insights into human co-transcriptional capping - structure 3 Deposited 2023-05-20 | Assembly 1 Other combination Heteromer；Protein × 15 PDB declaration: octadecameric（18） Consistent with all polymers |
Chain Y
1–117（117 aa）
|
Not recorded | ZN ZINC ION × 3 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 8P4D Structural insights into human co-transcriptional capping - structure 4 Deposited 2023-05-20 | Assembly 1 Other combination Heteromer；Protein × 15 PDB declaration: octadecameric（18） Consistent with all polymers |
Chain Y
1–117（117 aa）
|
Not recorded | ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 8P4F Structural insights into human co-transcriptional capping - structure 6 Deposited 2023-05-20 | Assembly 1 Other combination Heteromer；Protein × 15 PDB declaration: octadecameric（18） Consistent with all polymers |
Chain Y
1–117（117 aa）
|
Not recorded | MGT 7N-METHYL-8-HYDROGUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 8 MG MAGNESIUM ION × 1 SAM S-ADENOSYLMETHIONINE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.00 Å |
| 8UHA Structure of paused transcription complex Pol II-DSIF-NELF - tilted Deposited 2023-10-08 | Assembly 1 Other combination Heteromer；Protein × 18 PDB declaration: 21-meric（21） Consistent with all polymers |
Chain Y
1–117（117 aa）
|
Not recorded | ZN ZINC ION × 9 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 8UHD Structure of paused transcription complex Pol II-DSIF-NELF - post-translocated Deposited 2023-10-08 | Assembly 1 Other combination Heteromer；Protein × 18 PDB declaration: 21-meric（21） Consistent with all polymers |
Chain Y
1–117（117 aa）
|
Not recorded | ZN ZINC ION × 9 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.80 Å |
| 8W8E human co-transcriptional RNA capping enzyme RNGTT Deposited 2023-09-02 | Assembly 1 Other combination Heteromer；Protein × 19 PDB declaration: 22-meric（22） Consistent with all polymers |
Chain Y
1–117（117 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 3.90 Å |
| 8W8F human co-transcriptional RNA capping enzyme RNGTT-CMTR1 Deposited 2023-09-02 | Assembly 1 Other combination Heteromer；Protein × 16 PDB declaration: nonadecameric（19） Consistent with all polymers |
Chain Y
1–117（117 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 4.00 Å |
| 8XRM RNA polymerase II elongation complex with DSIF, SPT6, and ELOF1 transcribing genomic DNA extracted from human nuclei Deposited 2024-01-07 | Assembly 1 Other combination Heteromer；Protein × 16 PDB declaration: 19-meric（19） Consistent with all polymers |
Chain Y
1–117（117 aa）
|
Not recorded | ZN ZINC ION × 10 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.13 Å |
| 9EGX RNA polymerase II-DSIF-SPT6-PAF1c-TFIIS-IWS1-hexasome, bp +27 Deposited 2024-11-21 | Assembly 1 Other combination Heteromer；Protein × 29 PDB declaration: 32-meric（32） Consistent with all polymers |
Chain Y
1–117（117 aa）
|
Not recorded | ZN ZINC ION × 9 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 9EGY RNA polymerase II-DSIF-SPT6-PAF1c-TFIIS-IWS1-nucleosome, bp +27 Deposited 2024-11-21 | Assembly 1 Other combination Heteromer；Protein × 31 PDB declaration: 34-meric（34） Consistent with all polymers |
Chain Y
1–117（117 aa）
|
Not recorded | ZN ZINC ION × 9 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 9EGZ RNA polymerase II-DSIF-SPT6-PAF1c-TFIIS-IWS1-SETD2-nucleosome, bp +27 Deposited 2024-11-21 | Assembly 1 Other combination Heteromer；Protein × 32 PDB declaration: 35-meric（35） Consistent with all polymers |
Chain Y
1–117（117 aa）
|
Not recorded | ZN ZINC ION × 12 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 9EH0 RNA polymerase II-DSIF-SPT6-PAF1c-TFIIS-IWS1-SETD2-nucleosome, 30 bp upstream Deposited 2024-11-21 | Assembly 1 Other combination Heteromer；Protein × 32 PDB declaration: 35-meric（35） Consistent with all polymers |
Chain Y
1–117（117 aa）
|
Not recorded | ZN ZINC ION × 9 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 9EH1 RNA polymerase II-DSIF-SPT6-PAF1c-TFIIS-IWS1-SETD2-nucleosome, 20 bp upstream Deposited 2024-11-21 | Assembly 1 Other combination Heteromer；Protein × 32 PDB declaration: 35-meric（35） Consistent with all polymers |
Chain Y
2–117（116 aa）
|
Not recorded | ZN ZINC ION × 9 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 9EH2 RNA polymerase II-DSIF-SPT6-PAF1c-TFIIS-IWS1-SETD2-FACT nucleosome upstream Deposited 2024-11-21 | Assembly 1 Other combination Heteromer；Protein × 32 PDB declaration: 35-meric（35） Consistent with all polymers |
Chain Y
1–117（117 aa）
|
Not recorded | ZN ZINC ION × 9 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 9FYX Influenza A/H7N9 polymerase pre-cleavage cap-snatching complex Deposited 2024-07-04 | Assembly 1 Other combination Heteromer；Protein × 18 PDB declaration: 23-meric（23） Consistent with all polymers |
Chain Y
1–117（117 aa）
|
Not recorded | ZN ZINC ION × 9 G1G 7-METHYL-GUANOSINE-5'-TRIPHOSPHATE-5'-(2'-O-METHYL)-GUANOSINE × 1 MG MAGNESIUM ION × 2 PO4 PHOSPHATE ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8；20 mM BICINE pH 8.0
150 mM NaCl
0.1 mM MgCl2
4% glycerol
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 9G0A Influenza A/H7N9 polymerase post-cleavage cap-snatching complex Deposited 2024-07-07 | Assembly 1 Other combination Heteromer；Protein × 18 PDB declaration: 23-meric（23） Consistent with all polymers |
Chain Y
1–117（117 aa）
|
Not recorded | ZN ZINC ION × 8 MG MAGNESIUM ION × 2 G1G 7-METHYL-GUANOSINE-5'-TRIPHOSPHATE-5'-(2'-O-METHYL)-GUANOSINE × 1 PO4 PHOSPHATE ION × 3 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8；20 mM BICINE pH 8.0
150 mM NaCl
3 mM MgCl2
4% glycerol
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 9HVQ Structure of the transcribing Pol II-DSIF-PAF-SPT6-RECQL5 complex Deposited 2024-12-31 | Assembly 1 Other combination Heteromer；Protein × 21 PDB declaration: 24-meric（24） Consistent with all polymers |
Chain Y
1–117（117 aa）
|
Not recorded | ZN ZINC ION × 8 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5；20 mM HEPES pH 7.5, 50 mM KCl, 4 mM MgCl2, 1 mM DTT
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.00 Å |
| 9J0N Paused elongation complex of mammalian RNA polymerase II with nucleosome (PEC2-nuc) Deposited 2024-08-02 | Assembly 1 Other combination Heteromer；Protein × 27 PDB declaration: 30-meric（30） Consistent with all polymers |
Chain Y
1–117（117 aa）
|
Not recorded | ZN ZINC ION × 9 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 9J0O Arrested elongation complex of mammalian RNA polymerase II with nucleosome (AEC1-nuc) Deposited 2024-08-02 | Assembly 1 Other combination Heteromer；Protein × 26 PDB declaration: 29-meric（29） Consistent with all polymers |
Chain Y
1–117（117 aa）
|
Not recorded | ZN ZINC ION × 9 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 9J0P Arrested elongation complex of mammalian RNA polymerase II with nucleosome (AEC2-nuc) Deposited 2024-08-02 | Assembly 1 Other combination Heteromer；Protein × 26 PDB declaration: 29-meric（29） Consistent with all polymers |
Chain Y
1–117（117 aa）
|
Not recorded | ZN ZINC ION × 9 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 9MLC Pol II-DSIF-SPT6-PAF1c-TFIIS-IWS1-ELOF1-LEDGF-nucleosome activated elongation complex Composite map T Deposited 2024-12-19 | Assembly 1 Other combination Heteromer；Protein × 24 PDB declaration: 27-meric（27） Consistent with all polymers |
Chain Y
1–117（117 aa）
|
Not recorded | ZN ZINC ION × 10 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.40 Å |
| 9RTT Activated Elongation Complex with IWS1 and ELOF1 Deposited 2025-07-03 | Assembly 1 Other combination Heteromer；Protein × 22 PDB declaration: 25-meric（25） Consistent with all polymers |
Chain Y
1–117（117 aa）
|
Not recorded | ZN ZINC ION × 9 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.01 Å |
| 9VD9 Cryo-EM structure of the human DSS1-INTAC-PEC complex Deposited 2025-06-07 | Assembly 1 Insufficient information Heteromer；Protein × 35 PDB declaration: 38-meric（38） Consistent with all polymers |
Chain i
1–117（117 aa）
|
Not recorded | ZN ZINC ION × 10 MN MANGANESE (II) ION × 2 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.60 Å |