9eh1

RNA polymerase II-DSIF-SPT6-PAF1c-TFIIS-IWS1-SETD2-nucleosome, 20 bp upstream

Method: ELECTRON MICROSCOPY

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1. Protein Identity and Related Structures Protein Identity & Related Structures

DNA-directed RNA polymerase subunit

OrganismNot specified

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 32 DNA 2 RNA 1	DNA-directed RNA polymerase subunit beta × 1 （A0A4X1TVZ5） DNA-directed RNA polymerase II subunit RPB3 × 1 （A0A481DF93） RNA polymerase Rpb4/RPC9 core domain-containing protein × 1 （A0A8D0KES4） DNA-directed RNA polymerase II subunit E × 1 （A0A4X1VTX4） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （A0A8D1QTB4） DNA-directed RNA polymerase subunit × 1 （A0A4X1VKG7） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （I3LCB2） DNA-directed RNA polymerase II subunit RPB9 × 1 （P60899） DNA-directed RNA polymerase I, II, and III subunit RPABC5 × 1 DNA-directed RNA polymerase II subunit RPB11-a × 1 （F1RKE4） RNA polymerase II subunit K × 1 （A0A8D0JYF1） Transcription elongation factor SPT6 × 1 Non-template DNA × 1 Protein IWS1 homolog × 1 （Q96ST2） RNA × 1 RNA polymerase-associated protein CTR9 homolog × 1 （Q6PD62） RNA polymerase-associated protein RTF1 homolog × 1 （Q92541） Transcription elongation factor A protein 1 × 1 （P23193） Template DNA × 1 RNA polymerase-associated protein LEO1 × 1 RNA polymerase II-associated factor 1 homolog × 1 Superkiller complex protein 8, N-terminally processed × 1 （Q9GZS3） Parafibromin × 1 （Q6P1J9） Transcription elongation factor SPT4 × 1 （P63272） Transcription elongation factor SPT5 × 1 Histone H3 × 2 （A0A310TTQ1） Histone H4 × 2 （P62799） Histone H2A type 1 × 2 （P06897） Histone H2B 1.1 × 2 （P02281） Histone-lysine N-methyltransferase SETD2 × 1 （Q9BYW2） ZINC ION × 9 MAGNESIUM ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	A0A8D1DS11_PIG
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 1–1544； UniProt 10–1553

DNA-directed RNA polymerase subunit beta

OrganismNot specified

UniProt A0A4X1TVZ5

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 32 DNA 2 RNA 1	DNA-directed RNA polymerase subunit × 1 （A0A8D1DS11） DNA-directed RNA polymerase II subunit RPB3 × 1 （A0A481DF93） RNA polymerase Rpb4/RPC9 core domain-containing protein × 1 （A0A8D0KES4） DNA-directed RNA polymerase II subunit E × 1 （A0A4X1VTX4） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （A0A8D1QTB4） DNA-directed RNA polymerase subunit × 1 （A0A4X1VKG7） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （I3LCB2） DNA-directed RNA polymerase II subunit RPB9 × 1 （P60899） DNA-directed RNA polymerase I, II, and III subunit RPABC5 × 1 DNA-directed RNA polymerase II subunit RPB11-a × 1 （F1RKE4） RNA polymerase II subunit K × 1 （A0A8D0JYF1） Transcription elongation factor SPT6 × 1 Non-template DNA × 1 Protein IWS1 homolog × 1 （Q96ST2） RNA × 1 RNA polymerase-associated protein CTR9 homolog × 1 （Q6PD62） RNA polymerase-associated protein RTF1 homolog × 1 （Q92541） Transcription elongation factor A protein 1 × 1 （P23193） Template DNA × 1 RNA polymerase-associated protein LEO1 × 1 RNA polymerase II-associated factor 1 homolog × 1 Superkiller complex protein 8, N-terminally processed × 1 （Q9GZS3） Parafibromin × 1 （Q6P1J9） Transcription elongation factor SPT4 × 1 （P63272） Transcription elongation factor SPT5 × 1 Histone H3 × 2 （A0A310TTQ1） Histone H4 × 2 （P62799） Histone H2A type 1 × 2 （P06897） Histone H2B 1.1 × 2 （P02281） Histone-lysine N-methyltransferase SETD2 × 1 （Q9BYW2） ZINC ION × 9 MAGNESIUM ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	A0A4X1TVZ5_PIG
Isoform	—
PDB entities	2
Chains and sequence ranges	Author chain B； PDBConstruct 1–1159； UniProt 93–1251

DNA-directed RNA polymerase II subunit RPB3

OrganismNot specified

UniProt A0A481DF93

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 32 DNA 2 RNA 1	DNA-directed RNA polymerase subunit × 1 （A0A8D1DS11） DNA-directed RNA polymerase subunit beta × 1 （A0A4X1TVZ5） RNA polymerase Rpb4/RPC9 core domain-containing protein × 1 （A0A8D0KES4） DNA-directed RNA polymerase II subunit E × 1 （A0A4X1VTX4） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （A0A8D1QTB4） DNA-directed RNA polymerase subunit × 1 （A0A4X1VKG7） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （I3LCB2） DNA-directed RNA polymerase II subunit RPB9 × 1 （P60899） DNA-directed RNA polymerase I, II, and III subunit RPABC5 × 1 DNA-directed RNA polymerase II subunit RPB11-a × 1 （F1RKE4） RNA polymerase II subunit K × 1 （A0A8D0JYF1） Transcription elongation factor SPT6 × 1 Non-template DNA × 1 Protein IWS1 homolog × 1 （Q96ST2） RNA × 1 RNA polymerase-associated protein CTR9 homolog × 1 （Q6PD62） RNA polymerase-associated protein RTF1 homolog × 1 （Q92541） Transcription elongation factor A protein 1 × 1 （P23193） Template DNA × 1 RNA polymerase-associated protein LEO1 × 1 RNA polymerase II-associated factor 1 homolog × 1 Superkiller complex protein 8, N-terminally processed × 1 （Q9GZS3） Parafibromin × 1 （Q6P1J9） Transcription elongation factor SPT4 × 1 （P63272） Transcription elongation factor SPT5 × 1 Histone H3 × 2 （A0A310TTQ1） Histone H4 × 2 （P62799） Histone H2A type 1 × 2 （P06897） Histone H2B 1.1 × 2 （P02281） Histone-lysine N-methyltransferase SETD2 × 1 （Q9BYW2） ZINC ION × 9 MAGNESIUM ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	A0A481DF93_PIG
Isoform	—
PDB entities	3
Chains and sequence ranges	Author chain C； PDBConstruct 1–269； UniProt 47–315

RNA polymerase Rpb4/RPC9 core domain-containing protein

OrganismNot specified

UniProt A0A8D0KES4

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 32 DNA 2 RNA 1	DNA-directed RNA polymerase subunit × 1 （A0A8D1DS11） DNA-directed RNA polymerase subunit beta × 1 （A0A4X1TVZ5） DNA-directed RNA polymerase II subunit RPB3 × 1 （A0A481DF93） DNA-directed RNA polymerase II subunit E × 1 （A0A4X1VTX4） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （A0A8D1QTB4） DNA-directed RNA polymerase subunit × 1 （A0A4X1VKG7） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （I3LCB2） DNA-directed RNA polymerase II subunit RPB9 × 1 （P60899） DNA-directed RNA polymerase I, II, and III subunit RPABC5 × 1 DNA-directed RNA polymerase II subunit RPB11-a × 1 （F1RKE4） RNA polymerase II subunit K × 1 （A0A8D0JYF1） Transcription elongation factor SPT6 × 1 Non-template DNA × 1 Protein IWS1 homolog × 1 （Q96ST2） RNA × 1 RNA polymerase-associated protein CTR9 homolog × 1 （Q6PD62） RNA polymerase-associated protein RTF1 homolog × 1 （Q92541） Transcription elongation factor A protein 1 × 1 （P23193） Template DNA × 1 RNA polymerase-associated protein LEO1 × 1 RNA polymerase II-associated factor 1 homolog × 1 Superkiller complex protein 8, N-terminally processed × 1 （Q9GZS3） Parafibromin × 1 （Q6P1J9） Transcription elongation factor SPT4 × 1 （P63272） Transcription elongation factor SPT5 × 1 Histone H3 × 2 （A0A310TTQ1） Histone H4 × 2 （P62799） Histone H2A type 1 × 2 （P06897） Histone H2B 1.1 × 2 （P02281） Histone-lysine N-methyltransferase SETD2 × 1 （Q9BYW2） ZINC ION × 9 MAGNESIUM ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	A0A8D0KES4_PIG
Isoform	—
PDB entities	4
Chains and sequence ranges	Author chain D； PDBConstruct 1–126； UniProt 56–181

DNA-directed RNA polymerase II subunit E

OrganismNot specified

UniProt A0A4X1VTX4

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 32 DNA 2 RNA 1	DNA-directed RNA polymerase subunit × 1 （A0A8D1DS11） DNA-directed RNA polymerase subunit beta × 1 （A0A4X1TVZ5） DNA-directed RNA polymerase II subunit RPB3 × 1 （A0A481DF93） RNA polymerase Rpb4/RPC9 core domain-containing protein × 1 （A0A8D0KES4） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （A0A8D1QTB4） DNA-directed RNA polymerase subunit × 1 （A0A4X1VKG7） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （I3LCB2） DNA-directed RNA polymerase II subunit RPB9 × 1 （P60899） DNA-directed RNA polymerase I, II, and III subunit RPABC5 × 1 DNA-directed RNA polymerase II subunit RPB11-a × 1 （F1RKE4） RNA polymerase II subunit K × 1 （A0A8D0JYF1） Transcription elongation factor SPT6 × 1 Non-template DNA × 1 Protein IWS1 homolog × 1 （Q96ST2） RNA × 1 RNA polymerase-associated protein CTR9 homolog × 1 （Q6PD62） RNA polymerase-associated protein RTF1 homolog × 1 （Q92541） Transcription elongation factor A protein 1 × 1 （P23193） Template DNA × 1 RNA polymerase-associated protein LEO1 × 1 RNA polymerase II-associated factor 1 homolog × 1 Superkiller complex protein 8, N-terminally processed × 1 （Q9GZS3） Parafibromin × 1 （Q6P1J9） Transcription elongation factor SPT4 × 1 （P63272） Transcription elongation factor SPT5 × 1 Histone H3 × 2 （A0A310TTQ1） Histone H4 × 2 （P62799） Histone H2A type 1 × 2 （P06897） Histone H2B 1.1 × 2 （P02281） Histone-lysine N-methyltransferase SETD2 × 1 （Q9BYW2） ZINC ION × 9 MAGNESIUM ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	A0A4X1VTX4_PIG
Isoform	—
PDB entities	5
Chains and sequence ranges	Author chain E； PDBConstruct 1–209； UniProt 2–210

DNA-directed RNA polymerases I, II, and III subunit RPABC2

OrganismNot specified

UniProt A0A8D1QTB4

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 32 DNA 2 RNA 1	DNA-directed RNA polymerase subunit × 1 （A0A8D1DS11） DNA-directed RNA polymerase subunit beta × 1 （A0A4X1TVZ5） DNA-directed RNA polymerase II subunit RPB3 × 1 （A0A481DF93） RNA polymerase Rpb4/RPC9 core domain-containing protein × 1 （A0A8D0KES4） DNA-directed RNA polymerase II subunit E × 1 （A0A4X1VTX4） DNA-directed RNA polymerase subunit × 1 （A0A4X1VKG7） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （I3LCB2） DNA-directed RNA polymerase II subunit RPB9 × 1 （P60899） DNA-directed RNA polymerase I, II, and III subunit RPABC5 × 1 DNA-directed RNA polymerase II subunit RPB11-a × 1 （F1RKE4） RNA polymerase II subunit K × 1 （A0A8D0JYF1） Transcription elongation factor SPT6 × 1 Non-template DNA × 1 Protein IWS1 homolog × 1 （Q96ST2） RNA × 1 RNA polymerase-associated protein CTR9 homolog × 1 （Q6PD62） RNA polymerase-associated protein RTF1 homolog × 1 （Q92541） Transcription elongation factor A protein 1 × 1 （P23193） Template DNA × 1 RNA polymerase-associated protein LEO1 × 1 RNA polymerase II-associated factor 1 homolog × 1 Superkiller complex protein 8, N-terminally processed × 1 （Q9GZS3） Parafibromin × 1 （Q6P1J9） Transcription elongation factor SPT4 × 1 （P63272） Transcription elongation factor SPT5 × 1 Histone H3 × 2 （A0A310TTQ1） Histone H4 × 2 （P62799） Histone H2A type 1 × 2 （P06897） Histone H2B 1.1 × 2 （P02281） Histone-lysine N-methyltransferase SETD2 × 1 （Q9BYW2） ZINC ION × 9 MAGNESIUM ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	A0A8D1QTB4_PIG
Isoform	—
PDB entities	6
Chains and sequence ranges	Author chain F； PDBConstruct 1–78； UniProt 60–137

DNA-directed RNA polymerase subunit

OrganismNot specified

UniProt A0A4X1VKG7

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 32 DNA 2 RNA 1	DNA-directed RNA polymerase subunit × 1 （A0A8D1DS11） DNA-directed RNA polymerase subunit beta × 1 （A0A4X1TVZ5） DNA-directed RNA polymerase II subunit RPB3 × 1 （A0A481DF93） RNA polymerase Rpb4/RPC9 core domain-containing protein × 1 （A0A8D0KES4） DNA-directed RNA polymerase II subunit E × 1 （A0A4X1VTX4） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （A0A8D1QTB4） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （I3LCB2） DNA-directed RNA polymerase II subunit RPB9 × 1 （P60899） DNA-directed RNA polymerase I, II, and III subunit RPABC5 × 1 DNA-directed RNA polymerase II subunit RPB11-a × 1 （F1RKE4） RNA polymerase II subunit K × 1 （A0A8D0JYF1） Transcription elongation factor SPT6 × 1 Non-template DNA × 1 Protein IWS1 homolog × 1 （Q96ST2） RNA × 1 RNA polymerase-associated protein CTR9 homolog × 1 （Q6PD62） RNA polymerase-associated protein RTF1 homolog × 1 （Q92541） Transcription elongation factor A protein 1 × 1 （P23193） Template DNA × 1 RNA polymerase-associated protein LEO1 × 1 RNA polymerase II-associated factor 1 homolog × 1 Superkiller complex protein 8, N-terminally processed × 1 （Q9GZS3） Parafibromin × 1 （Q6P1J9） Transcription elongation factor SPT4 × 1 （P63272） Transcription elongation factor SPT5 × 1 Histone H3 × 2 （A0A310TTQ1） Histone H4 × 2 （P62799） Histone H2A type 1 × 2 （P06897） Histone H2B 1.1 × 2 （P02281） Histone-lysine N-methyltransferase SETD2 × 1 （Q9BYW2） ZINC ION × 9 MAGNESIUM ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	A0A4X1VKG7_PIG
Isoform	—
PDB entities	7
Chains and sequence ranges	Author chain G； PDBConstruct 1–171； UniProt 1–171

DNA-directed RNA polymerases I, II, and III subunit RPABC3

OrganismNot specified

UniProt I3LCB2

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 32 DNA 2 RNA 1	DNA-directed RNA polymerase subunit × 1 （A0A8D1DS11） DNA-directed RNA polymerase subunit beta × 1 （A0A4X1TVZ5） DNA-directed RNA polymerase II subunit RPB3 × 1 （A0A481DF93） RNA polymerase Rpb4/RPC9 core domain-containing protein × 1 （A0A8D0KES4） DNA-directed RNA polymerase II subunit E × 1 （A0A4X1VTX4） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （A0A8D1QTB4） DNA-directed RNA polymerase subunit × 1 （A0A4X1VKG7） DNA-directed RNA polymerase II subunit RPB9 × 1 （P60899） DNA-directed RNA polymerase I, II, and III subunit RPABC5 × 1 DNA-directed RNA polymerase II subunit RPB11-a × 1 （F1RKE4） RNA polymerase II subunit K × 1 （A0A8D0JYF1） Transcription elongation factor SPT6 × 1 Non-template DNA × 1 Protein IWS1 homolog × 1 （Q96ST2） RNA × 1 RNA polymerase-associated protein CTR9 homolog × 1 （Q6PD62） RNA polymerase-associated protein RTF1 homolog × 1 （Q92541） Transcription elongation factor A protein 1 × 1 （P23193） Template DNA × 1 RNA polymerase-associated protein LEO1 × 1 RNA polymerase II-associated factor 1 homolog × 1 Superkiller complex protein 8, N-terminally processed × 1 （Q9GZS3） Parafibromin × 1 （Q6P1J9） Transcription elongation factor SPT4 × 1 （P63272） Transcription elongation factor SPT5 × 1 Histone H3 × 2 （A0A310TTQ1） Histone H4 × 2 （P62799） Histone H2A type 1 × 2 （P06897） Histone H2B 1.1 × 2 （P02281） Histone-lysine N-methyltransferase SETD2 × 1 （Q9BYW2） ZINC ION × 9 MAGNESIUM ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	I3LCB2_PIG
Isoform	—
PDB entities	8
Chains and sequence ranges	Author chain H； PDBConstruct 1–149； UniProt 2–150

DNA-directed RNA polymerase II subunit RPB9

OrganismNot specified

UniProt P60899

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 32 DNA 2 RNA 1	DNA-directed RNA polymerase subunit × 1 （A0A8D1DS11） DNA-directed RNA polymerase subunit beta × 1 （A0A4X1TVZ5） DNA-directed RNA polymerase II subunit RPB3 × 1 （A0A481DF93） RNA polymerase Rpb4/RPC9 core domain-containing protein × 1 （A0A8D0KES4） DNA-directed RNA polymerase II subunit E × 1 （A0A4X1VTX4） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （A0A8D1QTB4） DNA-directed RNA polymerase subunit × 1 （A0A4X1VKG7） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （I3LCB2） DNA-directed RNA polymerase I, II, and III subunit RPABC5 × 1 DNA-directed RNA polymerase II subunit RPB11-a × 1 （F1RKE4） RNA polymerase II subunit K × 1 （A0A8D0JYF1） Transcription elongation factor SPT6 × 1 Non-template DNA × 1 Protein IWS1 homolog × 1 （Q96ST2） RNA × 1 RNA polymerase-associated protein CTR9 homolog × 1 （Q6PD62） RNA polymerase-associated protein RTF1 homolog × 1 （Q92541） Transcription elongation factor A protein 1 × 1 （P23193） Template DNA × 1 RNA polymerase-associated protein LEO1 × 1 RNA polymerase II-associated factor 1 homolog × 1 Superkiller complex protein 8, N-terminally processed × 1 （Q9GZS3） Parafibromin × 1 （Q6P1J9） Transcription elongation factor SPT4 × 1 （P63272） Transcription elongation factor SPT5 × 1 Histone H3 × 2 （A0A310TTQ1） Histone H4 × 2 （P62799） Histone H2A type 1 × 2 （P06897） Histone H2B 1.1 × 2 （P02281） Histone-lysine N-methyltransferase SETD2 × 1 （Q9BYW2） ZINC ION × 9 MAGNESIUM ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RPB9_PIG
Isoform	—
PDB entities	9
Chains and sequence ranges	Author chain I； PDBConstruct 1–116； UniProt 10–125

DNA-directed RNA polymerase II subunit RPB11-a

OrganismNot specified

UniProt F1RKE4

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 32 DNA 2 RNA 1	DNA-directed RNA polymerase subunit × 1 （A0A8D1DS11） DNA-directed RNA polymerase subunit beta × 1 （A0A4X1TVZ5） DNA-directed RNA polymerase II subunit RPB3 × 1 （A0A481DF93） RNA polymerase Rpb4/RPC9 core domain-containing protein × 1 （A0A8D0KES4） DNA-directed RNA polymerase II subunit E × 1 （A0A4X1VTX4） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （A0A8D1QTB4） DNA-directed RNA polymerase subunit × 1 （A0A4X1VKG7） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （I3LCB2） DNA-directed RNA polymerase II subunit RPB9 × 1 （P60899） DNA-directed RNA polymerase I, II, and III subunit RPABC5 × 1 RNA polymerase II subunit K × 1 （A0A8D0JYF1） Transcription elongation factor SPT6 × 1 Non-template DNA × 1 Protein IWS1 homolog × 1 （Q96ST2） RNA × 1 RNA polymerase-associated protein CTR9 homolog × 1 （Q6PD62） RNA polymerase-associated protein RTF1 homolog × 1 （Q92541） Transcription elongation factor A protein 1 × 1 （P23193） Template DNA × 1 RNA polymerase-associated protein LEO1 × 1 RNA polymerase II-associated factor 1 homolog × 1 Superkiller complex protein 8, N-terminally processed × 1 （Q9GZS3） Parafibromin × 1 （Q6P1J9） Transcription elongation factor SPT4 × 1 （P63272） Transcription elongation factor SPT5 × 1 Histone H3 × 2 （A0A310TTQ1） Histone H4 × 2 （P62799） Histone H2A type 1 × 2 （P06897） Histone H2B 1.1 × 2 （P02281） Histone-lysine N-methyltransferase SETD2 × 1 （Q9BYW2） ZINC ION × 9 MAGNESIUM ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	F1RKE4_PIG
Isoform	—
PDB entities	11
Chains and sequence ranges	Author chain K； PDBConstruct 1–115； UniProt 1–115

RNA polymerase II subunit K

OrganismNot specified

UniProt A0A8D0JYF1

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 32 DNA 2 RNA 1	DNA-directed RNA polymerase subunit × 1 （A0A8D1DS11） DNA-directed RNA polymerase subunit beta × 1 （A0A4X1TVZ5） DNA-directed RNA polymerase II subunit RPB3 × 1 （A0A481DF93） RNA polymerase Rpb4/RPC9 core domain-containing protein × 1 （A0A8D0KES4） DNA-directed RNA polymerase II subunit E × 1 （A0A4X1VTX4） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （A0A8D1QTB4） DNA-directed RNA polymerase subunit × 1 （A0A4X1VKG7） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （I3LCB2） DNA-directed RNA polymerase II subunit RPB9 × 1 （P60899） DNA-directed RNA polymerase I, II, and III subunit RPABC5 × 1 DNA-directed RNA polymerase II subunit RPB11-a × 1 （F1RKE4） Transcription elongation factor SPT6 × 1 Non-template DNA × 1 Protein IWS1 homolog × 1 （Q96ST2） RNA × 1 RNA polymerase-associated protein CTR9 homolog × 1 （Q6PD62） RNA polymerase-associated protein RTF1 homolog × 1 （Q92541） Transcription elongation factor A protein 1 × 1 （P23193） Template DNA × 1 RNA polymerase-associated protein LEO1 × 1 RNA polymerase II-associated factor 1 homolog × 1 Superkiller complex protein 8, N-terminally processed × 1 （Q9GZS3） Parafibromin × 1 （Q6P1J9） Transcription elongation factor SPT4 × 1 （P63272） Transcription elongation factor SPT5 × 1 Histone H3 × 2 （A0A310TTQ1） Histone H4 × 2 （P62799） Histone H2A type 1 × 2 （P06897） Histone H2B 1.1 × 2 （P02281） Histone-lysine N-methyltransferase SETD2 × 1 （Q9BYW2） ZINC ION × 9 MAGNESIUM ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	A0A8D0JYF1_PIG
Isoform	—
PDB entities	12
Chains and sequence ranges	Author chain L； PDBConstruct 1–47； UniProt 12–58

Protein IWS1 homolog

Homo sapiens

UniProt Q96ST2

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 32 DNA 2 RNA 1	DNA-directed RNA polymerase subunit × 1 （A0A8D1DS11） DNA-directed RNA polymerase subunit beta × 1 （A0A4X1TVZ5） DNA-directed RNA polymerase II subunit RPB3 × 1 （A0A481DF93） RNA polymerase Rpb4/RPC9 core domain-containing protein × 1 （A0A8D0KES4） DNA-directed RNA polymerase II subunit E × 1 （A0A4X1VTX4） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （A0A8D1QTB4） DNA-directed RNA polymerase subunit × 1 （A0A4X1VKG7） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （I3LCB2） DNA-directed RNA polymerase II subunit RPB9 × 1 （P60899） DNA-directed RNA polymerase I, II, and III subunit RPABC5 × 1 DNA-directed RNA polymerase II subunit RPB11-a × 1 （F1RKE4） RNA polymerase II subunit K × 1 （A0A8D0JYF1） Transcription elongation factor SPT6 × 1 Non-template DNA × 1 RNA × 1 RNA polymerase-associated protein CTR9 homolog × 1 （Q6PD62） RNA polymerase-associated protein RTF1 homolog × 1 （Q92541） Transcription elongation factor A protein 1 × 1 （P23193） Template DNA × 1 RNA polymerase-associated protein LEO1 × 1 RNA polymerase II-associated factor 1 homolog × 1 Superkiller complex protein 8, N-terminally processed × 1 （Q9GZS3） Parafibromin × 1 （Q6P1J9） Transcription elongation factor SPT4 × 1 （P63272） Transcription elongation factor SPT5 × 1 Histone H3 × 2 （A0A310TTQ1） Histone H4 × 2 （P62799） Histone H2A type 1 × 2 （P06897） Histone H2B 1.1 × 2 （P02281） Histone-lysine N-methyltransferase SETD2 × 1 （Q9BYW2） ZINC ION × 9 MAGNESIUM ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	IWS1_HUMAN
Isoform	—
PDB entities	15
Chains and sequence ranges	Author chain O； PDBConstruct 1–132； UniProt 554–685

RNA polymerase-associated protein CTR9 homolog

Homo sapiens

UniProt Q6PD62

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 32 DNA 2 RNA 1	DNA-directed RNA polymerase subunit × 1 （A0A8D1DS11） DNA-directed RNA polymerase subunit beta × 1 （A0A4X1TVZ5） DNA-directed RNA polymerase II subunit RPB3 × 1 （A0A481DF93） RNA polymerase Rpb4/RPC9 core domain-containing protein × 1 （A0A8D0KES4） DNA-directed RNA polymerase II subunit E × 1 （A0A4X1VTX4） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （A0A8D1QTB4） DNA-directed RNA polymerase subunit × 1 （A0A4X1VKG7） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （I3LCB2） DNA-directed RNA polymerase II subunit RPB9 × 1 （P60899） DNA-directed RNA polymerase I, II, and III subunit RPABC5 × 1 DNA-directed RNA polymerase II subunit RPB11-a × 1 （F1RKE4） RNA polymerase II subunit K × 1 （A0A8D0JYF1） Transcription elongation factor SPT6 × 1 Non-template DNA × 1 Protein IWS1 homolog × 1 （Q96ST2） RNA × 1 RNA polymerase-associated protein RTF1 homolog × 1 （Q92541） Transcription elongation factor A protein 1 × 1 （P23193） Template DNA × 1 RNA polymerase-associated protein LEO1 × 1 RNA polymerase II-associated factor 1 homolog × 1 Superkiller complex protein 8, N-terminally processed × 1 （Q9GZS3） Parafibromin × 1 （Q6P1J9） Transcription elongation factor SPT4 × 1 （P63272） Transcription elongation factor SPT5 × 1 Histone H3 × 2 （A0A310TTQ1） Histone H4 × 2 （P62799） Histone H2A type 1 × 2 （P06897） Histone H2B 1.1 × 2 （P02281） Histone-lysine N-methyltransferase SETD2 × 1 （Q9BYW2） ZINC ION × 9 MAGNESIUM ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	CTR9_HUMAN
Isoform	—
PDB entities	17
Chains and sequence ranges	Author chain Q； PDBConstruct 1–890； UniProt 3–892

RNA polymerase-associated protein RTF1 homolog

Homo sapiens

UniProt Q92541

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 32 DNA 2 RNA 1	DNA-directed RNA polymerase subunit × 1 （A0A8D1DS11） DNA-directed RNA polymerase subunit beta × 1 （A0A4X1TVZ5） DNA-directed RNA polymerase II subunit RPB3 × 1 （A0A481DF93） RNA polymerase Rpb4/RPC9 core domain-containing protein × 1 （A0A8D0KES4） DNA-directed RNA polymerase II subunit E × 1 （A0A4X1VTX4） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （A0A8D1QTB4） DNA-directed RNA polymerase subunit × 1 （A0A4X1VKG7） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （I3LCB2） DNA-directed RNA polymerase II subunit RPB9 × 1 （P60899） DNA-directed RNA polymerase I, II, and III subunit RPABC5 × 1 DNA-directed RNA polymerase II subunit RPB11-a × 1 （F1RKE4） RNA polymerase II subunit K × 1 （A0A8D0JYF1） Transcription elongation factor SPT6 × 1 Non-template DNA × 1 Protein IWS1 homolog × 1 （Q96ST2） RNA × 1 RNA polymerase-associated protein CTR9 homolog × 1 （Q6PD62） Transcription elongation factor A protein 1 × 1 （P23193） Template DNA × 1 RNA polymerase-associated protein LEO1 × 1 RNA polymerase II-associated factor 1 homolog × 1 Superkiller complex protein 8, N-terminally processed × 1 （Q9GZS3） Parafibromin × 1 （Q6P1J9） Transcription elongation factor SPT4 × 1 （P63272） Transcription elongation factor SPT5 × 1 Histone H3 × 2 （A0A310TTQ1） Histone H4 × 2 （P62799） Histone H2A type 1 × 2 （P06897） Histone H2B 1.1 × 2 （P02281） Histone-lysine N-methyltransferase SETD2 × 1 （Q9BYW2） ZINC ION × 9 MAGNESIUM ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RTF1_HUMAN
Isoform	—
PDB entities	18
Chains and sequence ranges	Author chain R； PDBConstruct 1–248； UniProt 353–600

Transcription elongation factor A protein 1

Homo sapiens

UniProt P23193

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 32 DNA 2 RNA 1	DNA-directed RNA polymerase subunit × 1 （A0A8D1DS11） DNA-directed RNA polymerase subunit beta × 1 （A0A4X1TVZ5） DNA-directed RNA polymerase II subunit RPB3 × 1 （A0A481DF93） RNA polymerase Rpb4/RPC9 core domain-containing protein × 1 （A0A8D0KES4） DNA-directed RNA polymerase II subunit E × 1 （A0A4X1VTX4） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （A0A8D1QTB4） DNA-directed RNA polymerase subunit × 1 （A0A4X1VKG7） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （I3LCB2） DNA-directed RNA polymerase II subunit RPB9 × 1 （P60899） DNA-directed RNA polymerase I, II, and III subunit RPABC5 × 1 DNA-directed RNA polymerase II subunit RPB11-a × 1 （F1RKE4） RNA polymerase II subunit K × 1 （A0A8D0JYF1） Transcription elongation factor SPT6 × 1 Non-template DNA × 1 Protein IWS1 homolog × 1 （Q96ST2） RNA × 1 RNA polymerase-associated protein CTR9 homolog × 1 （Q6PD62） RNA polymerase-associated protein RTF1 homolog × 1 （Q92541） Template DNA × 1 RNA polymerase-associated protein LEO1 × 1 RNA polymerase II-associated factor 1 homolog × 1 Superkiller complex protein 8, N-terminally processed × 1 （Q9GZS3） Parafibromin × 1 （Q6P1J9） Transcription elongation factor SPT4 × 1 （P63272） Transcription elongation factor SPT5 × 1 Histone H3 × 2 （A0A310TTQ1） Histone H4 × 2 （P62799） Histone H2A type 1 × 2 （P06897） Histone H2B 1.1 × 2 （P02281） Histone-lysine N-methyltransferase SETD2 × 1 （Q9BYW2） ZINC ION × 9 MAGNESIUM ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	TCEA1_HUMAN
Isoform	—
PDB entities	19
Chains and sequence ranges	Author chain S； PDBConstruct 1–170； UniProt 132–301

Superkiller complex protein 8, N-terminally processed

Homo sapiens

UniProt Q9GZS3

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 32 DNA 2 RNA 1	DNA-directed RNA polymerase subunit × 1 （A0A8D1DS11） DNA-directed RNA polymerase subunit beta × 1 （A0A4X1TVZ5） DNA-directed RNA polymerase II subunit RPB3 × 1 （A0A481DF93） RNA polymerase Rpb4/RPC9 core domain-containing protein × 1 （A0A8D0KES4） DNA-directed RNA polymerase II subunit E × 1 （A0A4X1VTX4） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （A0A8D1QTB4） DNA-directed RNA polymerase subunit × 1 （A0A4X1VKG7） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （I3LCB2） DNA-directed RNA polymerase II subunit RPB9 × 1 （P60899） DNA-directed RNA polymerase I, II, and III subunit RPABC5 × 1 DNA-directed RNA polymerase II subunit RPB11-a × 1 （F1RKE4） RNA polymerase II subunit K × 1 （A0A8D0JYF1） Transcription elongation factor SPT6 × 1 Non-template DNA × 1 Protein IWS1 homolog × 1 （Q96ST2） RNA × 1 RNA polymerase-associated protein CTR9 homolog × 1 （Q6PD62） RNA polymerase-associated protein RTF1 homolog × 1 （Q92541） Transcription elongation factor A protein 1 × 1 （P23193） Template DNA × 1 RNA polymerase-associated protein LEO1 × 1 RNA polymerase II-associated factor 1 homolog × 1 Parafibromin × 1 （Q6P1J9） Transcription elongation factor SPT4 × 1 （P63272） Transcription elongation factor SPT5 × 1 Histone H3 × 2 （A0A310TTQ1） Histone H4 × 2 （P62799） Histone H2A type 1 × 2 （P06897） Histone H2B 1.1 × 2 （P02281） Histone-lysine N-methyltransferase SETD2 × 1 （Q9BYW2） ZINC ION × 9 MAGNESIUM ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	SKI8_HUMAN
Isoform	—
PDB entities	23
Chains and sequence ranges	Author chain W； PDBConstruct 1–300； UniProt 5–304

Parafibromin

Homo sapiens

UniProt Q6P1J9

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 32 DNA 2 RNA 1	DNA-directed RNA polymerase subunit × 1 （A0A8D1DS11） DNA-directed RNA polymerase subunit beta × 1 （A0A4X1TVZ5） DNA-directed RNA polymerase II subunit RPB3 × 1 （A0A481DF93） RNA polymerase Rpb4/RPC9 core domain-containing protein × 1 （A0A8D0KES4） DNA-directed RNA polymerase II subunit E × 1 （A0A4X1VTX4） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （A0A8D1QTB4） DNA-directed RNA polymerase subunit × 1 （A0A4X1VKG7） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （I3LCB2） DNA-directed RNA polymerase II subunit RPB9 × 1 （P60899） DNA-directed RNA polymerase I, II, and III subunit RPABC5 × 1 DNA-directed RNA polymerase II subunit RPB11-a × 1 （F1RKE4） RNA polymerase II subunit K × 1 （A0A8D0JYF1） Transcription elongation factor SPT6 × 1 Non-template DNA × 1 Protein IWS1 homolog × 1 （Q96ST2） RNA × 1 RNA polymerase-associated protein CTR9 homolog × 1 （Q6PD62） RNA polymerase-associated protein RTF1 homolog × 1 （Q92541） Transcription elongation factor A protein 1 × 1 （P23193） Template DNA × 1 RNA polymerase-associated protein LEO1 × 1 RNA polymerase II-associated factor 1 homolog × 1 Superkiller complex protein 8, N-terminally processed × 1 （Q9GZS3） Transcription elongation factor SPT4 × 1 （P63272） Transcription elongation factor SPT5 × 1 Histone H3 × 2 （A0A310TTQ1） Histone H4 × 2 （P62799） Histone H2A type 1 × 2 （P06897） Histone H2B 1.1 × 2 （P02281） Histone-lysine N-methyltransferase SETD2 × 1 （Q9BYW2） ZINC ION × 9 MAGNESIUM ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	CDC73_HUMAN
Isoform	—
PDB entities	24
Chains and sequence ranges	Author chain X； PDBConstruct 1–43； UniProt 218–260

Transcription elongation factor SPT4

Homo sapiens

UniProt P63272

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 32 DNA 2 RNA 1	DNA-directed RNA polymerase subunit × 1 （A0A8D1DS11） DNA-directed RNA polymerase subunit beta × 1 （A0A4X1TVZ5） DNA-directed RNA polymerase II subunit RPB3 × 1 （A0A481DF93） RNA polymerase Rpb4/RPC9 core domain-containing protein × 1 （A0A8D0KES4） DNA-directed RNA polymerase II subunit E × 1 （A0A4X1VTX4） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （A0A8D1QTB4） DNA-directed RNA polymerase subunit × 1 （A0A4X1VKG7） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （I3LCB2） DNA-directed RNA polymerase II subunit RPB9 × 1 （P60899） DNA-directed RNA polymerase I, II, and III subunit RPABC5 × 1 DNA-directed RNA polymerase II subunit RPB11-a × 1 （F1RKE4） RNA polymerase II subunit K × 1 （A0A8D0JYF1） Transcription elongation factor SPT6 × 1 Non-template DNA × 1 Protein IWS1 homolog × 1 （Q96ST2） RNA × 1 RNA polymerase-associated protein CTR9 homolog × 1 （Q6PD62） RNA polymerase-associated protein RTF1 homolog × 1 （Q92541） Transcription elongation factor A protein 1 × 1 （P23193） Template DNA × 1 RNA polymerase-associated protein LEO1 × 1 RNA polymerase II-associated factor 1 homolog × 1 Superkiller complex protein 8, N-terminally processed × 1 （Q9GZS3） Parafibromin × 1 （Q6P1J9） Transcription elongation factor SPT5 × 1 Histone H3 × 2 （A0A310TTQ1） Histone H4 × 2 （P62799） Histone H2A type 1 × 2 （P06897） Histone H2B 1.1 × 2 （P02281） Histone-lysine N-methyltransferase SETD2 × 1 （Q9BYW2） ZINC ION × 9 MAGNESIUM ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	SPT4H_HUMAN
Isoform	—
PDB entities	25
Chains and sequence ranges	Author chain Y； PDBConstruct 1–116； UniProt 2–117

Histone H3

Xenopus laevis

UniProt A0A310TTQ1

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 32 DNA 2 RNA 1	DNA-directed RNA polymerase subunit × 1 （A0A8D1DS11） DNA-directed RNA polymerase subunit beta × 1 （A0A4X1TVZ5） DNA-directed RNA polymerase II subunit RPB3 × 1 （A0A481DF93） RNA polymerase Rpb4/RPC9 core domain-containing protein × 1 （A0A8D0KES4） DNA-directed RNA polymerase II subunit E × 1 （A0A4X1VTX4） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （A0A8D1QTB4） DNA-directed RNA polymerase subunit × 1 （A0A4X1VKG7） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （I3LCB2） DNA-directed RNA polymerase II subunit RPB9 × 1 （P60899） DNA-directed RNA polymerase I, II, and III subunit RPABC5 × 1 DNA-directed RNA polymerase II subunit RPB11-a × 1 （F1RKE4） RNA polymerase II subunit K × 1 （A0A8D0JYF1） Transcription elongation factor SPT6 × 1 Non-template DNA × 1 Protein IWS1 homolog × 1 （Q96ST2） RNA × 1 RNA polymerase-associated protein CTR9 homolog × 1 （Q6PD62） RNA polymerase-associated protein RTF1 homolog × 1 （Q92541） Transcription elongation factor A protein 1 × 1 （P23193） Template DNA × 1 RNA polymerase-associated protein LEO1 × 1 RNA polymerase II-associated factor 1 homolog × 1 Superkiller complex protein 8, N-terminally processed × 1 （Q9GZS3） Parafibromin × 1 （Q6P1J9） Transcription elongation factor SPT4 × 1 （P63272） Transcription elongation factor SPT5 × 1 Histone H4 × 2 （P62799） Histone H2A type 1 × 2 （P06897） Histone H2B 1.1 × 2 （P02281） Histone-lysine N-methyltransferase SETD2 × 1 （Q9BYW2） ZINC ION × 9 MAGNESIUM ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	A0A310TTQ1_XENLA
Isoform	—
PDB entities	27
Chains and sequence ranges	Author chain a； PDBConstruct 1–136； UniProt 1–136 Author chain e； PDBConstruct 1–136； UniProt 1–136

Histone H4

Xenopus laevis

UniProt P62799

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 32 DNA 2 RNA 1	DNA-directed RNA polymerase subunit × 1 （A0A8D1DS11） DNA-directed RNA polymerase subunit beta × 1 （A0A4X1TVZ5） DNA-directed RNA polymerase II subunit RPB3 × 1 （A0A481DF93） RNA polymerase Rpb4/RPC9 core domain-containing protein × 1 （A0A8D0KES4） DNA-directed RNA polymerase II subunit E × 1 （A0A4X1VTX4） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （A0A8D1QTB4） DNA-directed RNA polymerase subunit × 1 （A0A4X1VKG7） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （I3LCB2） DNA-directed RNA polymerase II subunit RPB9 × 1 （P60899） DNA-directed RNA polymerase I, II, and III subunit RPABC5 × 1 DNA-directed RNA polymerase II subunit RPB11-a × 1 （F1RKE4） RNA polymerase II subunit K × 1 （A0A8D0JYF1） Transcription elongation factor SPT6 × 1 Non-template DNA × 1 Protein IWS1 homolog × 1 （Q96ST2） RNA × 1 RNA polymerase-associated protein CTR9 homolog × 1 （Q6PD62） RNA polymerase-associated protein RTF1 homolog × 1 （Q92541） Transcription elongation factor A protein 1 × 1 （P23193） Template DNA × 1 RNA polymerase-associated protein LEO1 × 1 RNA polymerase II-associated factor 1 homolog × 1 Superkiller complex protein 8, N-terminally processed × 1 （Q9GZS3） Parafibromin × 1 （Q6P1J9） Transcription elongation factor SPT4 × 1 （P63272） Transcription elongation factor SPT5 × 1 Histone H3 × 2 （A0A310TTQ1） Histone H2A type 1 × 2 （P06897） Histone H2B 1.1 × 2 （P02281） Histone-lysine N-methyltransferase SETD2 × 1 （Q9BYW2） ZINC ION × 9 MAGNESIUM ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	H4_XENLA
Isoform	—
PDB entities	28
Chains and sequence ranges	Author chain b； PDBConstruct 1–78； UniProt 25–102 Author chain f； PDBConstruct 1–78； UniProt 25–102

Histone H2A type 1

Xenopus laevis

UniProt P06897

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 32 DNA 2 RNA 1	DNA-directed RNA polymerase subunit × 1 （A0A8D1DS11） DNA-directed RNA polymerase subunit beta × 1 （A0A4X1TVZ5） DNA-directed RNA polymerase II subunit RPB3 × 1 （A0A481DF93） RNA polymerase Rpb4/RPC9 core domain-containing protein × 1 （A0A8D0KES4） DNA-directed RNA polymerase II subunit E × 1 （A0A4X1VTX4） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （A0A8D1QTB4） DNA-directed RNA polymerase subunit × 1 （A0A4X1VKG7） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （I3LCB2） DNA-directed RNA polymerase II subunit RPB9 × 1 （P60899） DNA-directed RNA polymerase I, II, and III subunit RPABC5 × 1 DNA-directed RNA polymerase II subunit RPB11-a × 1 （F1RKE4） RNA polymerase II subunit K × 1 （A0A8D0JYF1） Transcription elongation factor SPT6 × 1 Non-template DNA × 1 Protein IWS1 homolog × 1 （Q96ST2） RNA × 1 RNA polymerase-associated protein CTR9 homolog × 1 （Q6PD62） RNA polymerase-associated protein RTF1 homolog × 1 （Q92541） Transcription elongation factor A protein 1 × 1 （P23193） Template DNA × 1 RNA polymerase-associated protein LEO1 × 1 RNA polymerase II-associated factor 1 homolog × 1 Superkiller complex protein 8, N-terminally processed × 1 （Q9GZS3） Parafibromin × 1 （Q6P1J9） Transcription elongation factor SPT4 × 1 （P63272） Transcription elongation factor SPT5 × 1 Histone H3 × 2 （A0A310TTQ1） Histone H4 × 2 （P62799） Histone H2B 1.1 × 2 （P02281） Histone-lysine N-methyltransferase SETD2 × 1 （Q9BYW2） ZINC ION × 9 MAGNESIUM ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	H2A1_XENLA
Isoform	—
PDB entities	29
Chains and sequence ranges	Author chain c； PDBConstruct 1–130； UniProt 1–130 Author chain g； PDBConstruct 1–130； UniProt 1–130

Histone H2B 1.1

Xenopus laevis

UniProt P02281

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 32 DNA 2 RNA 1	DNA-directed RNA polymerase subunit × 1 （A0A8D1DS11） DNA-directed RNA polymerase subunit beta × 1 （A0A4X1TVZ5） DNA-directed RNA polymerase II subunit RPB3 × 1 （A0A481DF93） RNA polymerase Rpb4/RPC9 core domain-containing protein × 1 （A0A8D0KES4） DNA-directed RNA polymerase II subunit E × 1 （A0A4X1VTX4） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （A0A8D1QTB4） DNA-directed RNA polymerase subunit × 1 （A0A4X1VKG7） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （I3LCB2） DNA-directed RNA polymerase II subunit RPB9 × 1 （P60899） DNA-directed RNA polymerase I, II, and III subunit RPABC5 × 1 DNA-directed RNA polymerase II subunit RPB11-a × 1 （F1RKE4） RNA polymerase II subunit K × 1 （A0A8D0JYF1） Transcription elongation factor SPT6 × 1 Non-template DNA × 1 Protein IWS1 homolog × 1 （Q96ST2） RNA × 1 RNA polymerase-associated protein CTR9 homolog × 1 （Q6PD62） RNA polymerase-associated protein RTF1 homolog × 1 （Q92541） Transcription elongation factor A protein 1 × 1 （P23193） Template DNA × 1 RNA polymerase-associated protein LEO1 × 1 RNA polymerase II-associated factor 1 homolog × 1 Superkiller complex protein 8, N-terminally processed × 1 （Q9GZS3） Parafibromin × 1 （Q6P1J9） Transcription elongation factor SPT4 × 1 （P63272） Transcription elongation factor SPT5 × 1 Histone H3 × 2 （A0A310TTQ1） Histone H4 × 2 （P62799） Histone H2A type 1 × 2 （P06897） Histone-lysine N-methyltransferase SETD2 × 1 （Q9BYW2） ZINC ION × 9 MAGNESIUM ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	H2B11_XENLA
Isoform	—
PDB entities	30
Chains and sequence ranges	Author chain d； PDBConstruct 2–123； UniProt 5–126 Author chain h； PDBConstruct 2–123； UniProt 5–126

Histone-lysine N-methyltransferase SETD2

Homo sapiens

UniProt Q9BYW2

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 32 DNA 2 RNA 1	DNA-directed RNA polymerase subunit × 1 （A0A8D1DS11） DNA-directed RNA polymerase subunit beta × 1 （A0A4X1TVZ5） DNA-directed RNA polymerase II subunit RPB3 × 1 （A0A481DF93） RNA polymerase Rpb4/RPC9 core domain-containing protein × 1 （A0A8D0KES4） DNA-directed RNA polymerase II subunit E × 1 （A0A4X1VTX4） DNA-directed RNA polymerases I, II, and III subunit RPABC2 × 1 （A0A8D1QTB4） DNA-directed RNA polymerase subunit × 1 （A0A4X1VKG7） DNA-directed RNA polymerases I, II, and III subunit RPABC3 × 1 （I3LCB2） DNA-directed RNA polymerase II subunit RPB9 × 1 （P60899） DNA-directed RNA polymerase I, II, and III subunit RPABC5 × 1 DNA-directed RNA polymerase II subunit RPB11-a × 1 （F1RKE4） RNA polymerase II subunit K × 1 （A0A8D0JYF1） Transcription elongation factor SPT6 × 1 Non-template DNA × 1 Protein IWS1 homolog × 1 （Q96ST2） RNA × 1 RNA polymerase-associated protein CTR9 homolog × 1 （Q6PD62） RNA polymerase-associated protein RTF1 homolog × 1 （Q92541） Transcription elongation factor A protein 1 × 1 （P23193） Template DNA × 1 RNA polymerase-associated protein LEO1 × 1 RNA polymerase II-associated factor 1 homolog × 1 Superkiller complex protein 8, N-terminally processed × 1 （Q9GZS3） Parafibromin × 1 （Q6P1J9） Transcription elongation factor SPT4 × 1 （P63272） Transcription elongation factor SPT5 × 1 Histone H3 × 2 （A0A310TTQ1） Histone H4 × 2 （P62799） Histone H2A type 1 × 2 （P06897） Histone H2B 1.1 × 2 （P02281） ZINC ION × 9 MAGNESIUM ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	SETD2_HUMAN
Isoform	—
PDB entities	31
Chains and sequence ranges	Author chain l； PDBConstruct 1–589； UniProt 1452–2040

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	9eh1
Deposition date deposition_date	2024-11-21
Structure title title	RNA polymerase II-DSIF-SPT6-PAF1c-TFIIS-IWS1-SETD2-nucleosome, 20 bp upstream
Keywords keywords	SETD2, Transcription, H3K36me3, TRANSFERASE-RNA-DNA complex; TRANSCRIPTION, TRANSFERASE/RNA/DNA
Experimental Method method	ELECTRON MICROSCOPY

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

9eh1__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

9eh1__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

9eh1__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	94.13 Å
Rg (electron density)	92.83 Å
Total Rg	93.14 Å
Atom count	72243
Residues	9011
Excluded volume	1246700 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`9eh1__assembly_1__model_1`	35-meric （35）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (33)

Entity 1 Entity 1 polymer

Description description	DNA-directed RNA polymerase subunit
Molecular weight formula_weight	173530.1 kDa
EC 编号 ec_number	2.7.7.6
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A
Atom count atom_count	72243

DSACPLRTIKRVQFGVLSPDELKRMSVTEGGIKYPETTEGGRPKLGGLMDPRQGVIERTGRCQTCAGNMTECPGHFGHIELAKPVFHVGFLVKTMKVLRCVCFFCSKLLVDSNNPKIKDILAKSKGQPKKRLTHVYDLCKGKNICEGGEEMDNKFGVEQPEGDEDLTKEKGHGGCGRYQPRIRRSGLELYAEWKHVNEDSQEKKILLSPERVHEIFKRISDEECFVLGMEPRYARPEWMIVTVLPVPPLSVRPAVVMQGSARNQDDLTHKLADIVKINNQLRRNEQNGAAAHVIAEDVKLLQFHVATMVDNELPGLPRAMQKSGRPLKSLKQRLKGKEGRVRGNLMGKRVDFSARTVITPDPNLSIDQVGVPRSIAANMTFAEIVTPFNIDRLQELVRRGNSQYPGAKYIIRDNGDRIDLRFHPKPSDLHLQTGYKVERHMCDGDIVIFNRQPTLHKMSMMGHRVRILPWSTFRLNLSVTTPYNADFDGDEMNLHLPQSLETRAEIQELAMVPRMIVTPQSNRPVMGIVQDTLTAVRKFTKRDVFLERGEVMNLLMFLSTWDGKVPQPAILKPRPLWTGKQIFSLIIPGHINCIRTHSTHPDDEDSGPYKHISPGDTKVVVENGELIMGILCKKSLGTSAGSLVHISYLEMGHDITRLFYSNIQTVINNWLLIEGHTIGIGDSIADSKTYQDIQNTIKKAKQDVIEVIEKAHNNELEPTPGNTLRQTFENQVNRILNDARDKTGSSAQKSLSEYNNFKSMVVSGAKGSKINISQVIAVVGQQNVEGKRIPFGFKHRTLPHFIKDDYGPESRGFVENSYLAGLTPTEFFFHAMGGREGLIDTAVKTAETGYIQRRLIKSMESVMVKYDATVRNSINQVVQLRYGEDGLAGESVEFQNLATLKPSNKAFEKKFRFDYTNERALRRTLQEDLVKDVLSNAHIQNELEREFERMREDREVLRVIFPTGDSKVVLPCNLLRMIWNAQKIFHINPRLPSDLHPIKVVEGVKELSKKLVIVNGDDPLSRQAQENATLLFNIHLRSTLCSRRMAEEFRLSGEAFDWLLGEIESKFNQAIAHPGEMVGALAAQSLGEPATQMTLNTFHYAGVSAKNVTLGVPRLKELINISKKPKTPSLTVFLLGQSARDAERAKDILCRLEHTTLRKVTANTAIYYDPNPQSTVVAEDQEWVNVYYEMPDFDVARISPWLLRVELDRKHMTDRKLTMEQIAEKINAGFGDDLNCIFNDDNAEKLVLRIRIMNSDENKMQEEEEVVDKMDDDVFLRCIESNMLTDMTLQGIEQISKVYMHLPQTDNKKKIIITEDGEFKALQEWILETDGVSLMRVLSEKDVDPVRTTSNDIVEIFTVLGIEAVRKALERELYHVISFDGSYVNYRHLALLCDTMTCRGHLMAITRHGVNRQDTGPLMKCSFEETVDVLMEAAAHGESDPMKGVSENIMLGQLAPAGTGCFDLLLDAEKCKYGMEIPTNIPGLGAAGPTGMFFGSAPSPMGGISPAMTPWNQGATPAYGAWSPSVGSGMTPGAAGFSPSAASD

Entity 2 Entity 2 polymer

Description description	DNA-directed RNA polymerase subunit beta
Molecular weight formula_weight	132189.7 kDa
EC 编号 ec_number	2.7.7.6
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	B

EITPDLWQEACWIVISSYFDEKGLVRQQLDSFDEFIQMSVQRIVEDAPPIDLQAEAQHASGEVEEPPRYLLKFEQIYLSKPTHWERDGAPSPMMPNEARLRNLTYSAPLYVDITKTVIKEGEEQLQTQHQKTFIGKIPIMLRSTYCLLNGLTDRDLCELNECPLDPGGYFIINGSEKVLIAQEKMATNTVYVFAKKDSKYAYTGECRSCLENSSRPTSTIWVSMLARGGQGAKKSAIGQRIVATLPYIKQEVPIIIVFRALGFVSDRDILEHIIYDFEDPEMMEMVKPSLDEAFVIQEQNVALNFIGSRGAKPGVTKEKRIKYAKEVLQKEMLPHVGVSDFCETKKAYFLGYMVHRLLLAALGRRELDDRDHYGNKRLDLAGPLLAFLFRGMFKNLLKEVRIYAQKFIDRGKDFNLELAIKTRIISDGLKYSLATGNWGDQKKAHQARAGVSQVLNRLTFASTLSHLRRLNSPIGRDGKLAKPRQLHNTLWGMVCPAETPEGHAVGLVKNLALMAYISVGSQPSPILEFLEEWSMENLEEISPAAIADATKIFVNGCWVGIHKDPEQLMNTLRKLRRQMDIIVSEVSMIRDIREREIRIYTDAGRICRPLLIVEKQKLLLKKRHIDQLKEREYNNYSWQDLVASGVVEYIDTLEEETVMLAMTPDDLQEKEVAYCSTYTHCEIHPSMILGVCASIIPFPDHNQSPRNTYQSAMGKQAMGVYITNFHVRMDTLAHVLYYPQKPLVTTRSMEYLRFRELPAGINSIVAIASYTGYNQEDSVIMNRSAVDRGFFRSVFYRSYKEQESKKGFDQEEVFEKPTRETCQGMRHAIYDKLDDDGLIAPGVRVSGDDVIIGKTVTLPENEDELEGTNRRYTKRDCSTFLRTSETGIVDQVMVTLNQEGYKFCKIRVRSVRIPQIGDKFASRHGQKGTCGIQYRQEDMPFTCEGITPDIIINPHAIPSRMTIGHLIECLQGKVSANKGEIGDATPFNDAVNVQKISNLLSDYGYHLRGNEVLYNGFTGRKITSQIFIGPTYYQRLKHMVDDKIHSRARGPIQILNRQPMEGRSRDGGLRFGEMERDCQIAHGAAQFLRERLFEASDPYQVHVCNLCGIMAIANTRTHTYECRGCRNKTQISLVRMPYACKLLFQELMSMSIAPRMMSV

Entity 3 Entity 3 polymer

Description description	DNA-directed RNA polymerase II subunit RPB3
Molecular weight formula_weight	30767.2 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	C

PYANQPTVRITELTDENVKFIIENTDLAVANSIRRVFIAEVPIIAIDWVQIDANSSVLHDEFIAHRLGLIPLTSDDIVDKLQYSRDCTCEEFCPECSVEFTLDVRCNEDQTRHVTSRDLISNSPRVIPVTSRNRDNDPSDYVEQDDILIVKLRKGQELRLRAYAKKGFGKEHAKWNPTAGVAFEYDPDNALRHTVYPKPEEWPKSEYSELDEDESQAPYDPNGKPERFYYNVESCGSLRPETIVLSALSGLKKKLSDLQTQLSHEIQSD

Entity 4 Entity 4 polymer

Description description	RNA polymerase Rpb4/RPC9 core domain-containing protein
Molecular weight formula_weight	14706.5 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	D

EEDASQLIFPKEFETAETLLNSEVHMLLEHRKQQNESAEDEQELSEVFMKTLNYTARFSRFKNRETIASVRSLLLQKKLHKFELACLANLCPETAEESKALIPSLEGRFEDEELQQILDDIQTKRS

Entity 5 Entity 5 polymer

Description description	DNA-directed RNA polymerase II subunit E
Molecular weight formula_weight	24513.1 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	E

DDEEETYRLWKIRKTIMQLCHDRGYLVTQDELDQTLEEFKAQFGDKPSEGRPRRTDLTVLVAHNDDPTDQMFVFFPEEPKVGIKTIKVYCQRMQEENITRALIVVQQGMTPSAKQSLVDMAPKYILEQFLQQELLINITEHELVPEHVVMTKEEVTELLARYKLRENQLPRIQAGDPVARYFGIKRGQVVKIIRPSETAGRYITYRLVQ

Entity 6 Entity 6 polymer

Description description	DNA-directed RNA polymerases I, II, and III subunit RPABC2
Molecular weight formula_weight	8978.6 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	F

KRITTPYMTKYERARVLGTRALQIAMCAPVMVELEGETDPLLIAMKELKARKIPIIIRRYLPDGSYEDWGVDELIISD

Entity 7 Entity 7 polymer

Description description	DNA-directed RNA polymerase subunit
Molecular weight formula_weight	19227.2 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	G

MFYHISLEHEILLHPRYFGPNLLNTVKQKLFTEVEGTCTGKYGFVIAVTTIDNIGAGVIQPGRGFVLYPVKYKAIVFRPFKGEVVDAVVTQVNKVGLFTEIGPMSCFISRHSIPSEMEFDPNSNPPCYKTMDEDIVIQQDDEIRLKIVGTRVDKNDIFAIGSLMDDYLGLV

Entity 8 Entity 8 polymer

Description description	DNA-directed RNA polymerases I, II, and III subunit RPABC3
Molecular weight formula_weight	17031.1 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	H

AGILFEDIFDVKDIDPEGKKFDRVSRLHCESESFKMDLILDVNIQIYPVDLGDKFRLVIASTLYEDGTLDDGEYNPTDDRPSRADQFEYVMYGKVYRIEGDETSTEAATRLSAYVSYGGLLMRLQGDANNLHGFEVDSRVYLLMKKLAF

Entity 9 Entity 9 polymer

Description description	DNA-directed RNA polymerase II subunit RPB9
Molecular weight formula_weight	13521.2 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	I

GFVGIRFCQECNNMLYPKEDKENRILLYACRNCDYQQEADNSCIYVNKITHEVDELTQIIADVSQDPTLPRTEDHPCQKCGHKEAVFFQSHSARAEDAMRLYYVCTAPHCGHRWTE

Entity 10 Entity 10 polymer

Description description	DNA-directed RNA polymerase I, II, and III subunit RPABC5
Molecular weight formula_weight	7525.9 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	J

MIIPVRCFTCGKIVGNKWEAYLGLLQAEYTEGDALDALGLKRYCCRRMLLAHVDLIEKLLNYAPLE

Entity 11 Entity 11 polymer

Description description	DNA-directed RNA polymerase II subunit RPB11-a
Molecular weight formula_weight	13068.0 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	K

MNAPPAFESFLLFEGEKKITINKDTKVPNACLFTINKEDHTLGNIIKSQLLKDPQVLFAGYKVPHPLEHKIIIRVQTTPDYSPQEAFTNAITDLISELSLLEERFRVAIKDKQEG

Entity 12 Entity 12 polymer

Description description	RNA polymerase II subunit K
Molecular weight formula_weight	5747.8 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	L

QQPMIYICGECHTENEIKSRDPIRCRECGYRIMYKKRTKRLVVFDAR

Entity 13 Entity 13 polymer

Description description	Transcription elongation factor SPT6
Molecular weight formula_weight	117027.7 kDa
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	M

DQDNEIRATDLPERFQLRSIPVKGAEDDELEEEADWIYRNAFATPTISLSRKGPSTIQKIKEALGFMRNQHFEVPFIAFYRKEYVEPELHINDLWRVWQWDEKWTQLRIRKENLTRLFEKMQAYQYEQISAIRALDTTDMERLKDVQSMDELKDVYNHFLLYYGRDIPKKFGLTPEQFGENLRDSYQRHETEQFPAEPLELAKDTPEAVLEGARYMVALQIAREPLVRQVLRQTFQERAKLNITPTKKGRKDVDEAHYAYSFKYLKNKPVKELRDDQFLKICLAEDEGLLTTDISIDLKGTYFEEIKQFYYRDEFSHQVQEWNRQRTMAIERALQQFLYVQMAKELKNKLLAEAKEYVIKACSRKLYNWLRVAPYRPDQQQGKGIRVLGIAFSSARDHPVFCALVNGEGEVTDFLRLPHFTEEREKKAQDIETLKKFLLNKKPHVVTVAGENRDAQMLIEDVKRIVHELDQGQQLSSIGVELVDNELAILYMNSKKSEAEFRDYPPVLRQAVSLARRIQDPLIEFAQVCSEDILCLKFHPLQEHVVKEELLNALYCEFINRVNEVGVDVNRAIAHPYSQALIQYVCGLGPRKGTHLLKILKQNNTRLESRTQLVTMCHMGPKVFMNCAGFLKIDTEVLDGSRVHPETYEWARKMAVDALEYDESAEDANPAGALEEILENPERLKDLDLDAFAEELERQGYGDKHITLYDIRAELSCRYKDLRTAYRSPNTEEIFNMLTKETPETFYIGKLIICNVTGIAHIGVKTRLDNGVTGFIPTKFLSDKVVKRPEERVKVGMTVHCRIMKIDIEKFSADLTCRTSDLMIAHPSFHNINFKQAEKMMETMDQGDVIIRPSSKGENHLTVTWKVSDGIYQHVDVRATLWINSEEFEDLDEIVARYVQPMASFARDLLNHKYYQDCSGGDRKKLEELLIKTKKEKPTFIPYFICACKELPGKFLLGYQPRGKPRIEYVTVTPEGFRYRGQIFPTVNGLFRWFKDHYQDPV

Source Source

Source organism organism	Homo sapiens
NCBI taxonomy ID ncbi_taxonomy_id	9606
Host organism host_organism	Trichoplusia ni

Entity 14 Entity 14 polymer

Description description	Non-template DNA
Molecular weight formula_weight	52348.3 kDa
Source method src_method	syn
Copy count entity_count	1

Polymer Polymer

Polymer type type	polydeoxyribonucleotide
Chain IDs strand_id	N

CAGAATCCCGGTGCCGAGGCCGCTCAATTGGTCGTAGACAGCTCTAGCACCGCTTAAACGCACGTACGCGCTGTCCCCCGCGTTTTAACCGCCAAGGGGATTACTCCCTAGTCTCCAGGCACGTGTCAGATATATACATCGATAACGGCCAGTTGTGTTTGGTGTGTTTG

Entity 15 Entity 15 polymer

Description description	Protein IWS1 homolog
Molecular weight formula_weight	14972.5 kDa
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	O

SDADDVVSAMIVKMNEAAEEDRQLNNQKKPALKKLTLLPAVVMHLKKQDLKETFIDSGVMSAIKEWLSPLPDRSLPALKIREELLKILQELPSVSQETLKHSGIGRAVMYLYKHPKESRSNKDMAGKLINEW

Source Source

Source organism organism	Homo sapiens
Common name common_name	human
NCBI taxonomy ID ncbi_taxonomy_id	9606
Host organism host_organism	Trichoplusia ni

Entity 16 Entity 16 polymer

Description description	RNA
Molecular weight formula_weight	3475.1 kDa
Source method src_method	syn
Copy count entity_count	1

Polymer Polymer

Polymer type type	polyribonucleotide
Chain IDs strand_id	P

AAAAUUAGCUC

Entity 17 Entity 17 polymer

Description description	RNA polymerase-associated protein CTR9 homolog
Molecular weight formula_weight	102859.1 kDa
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	Q

RGSIEIPLRDTDEVIELDFDQLPEGDEVISILKQEHTQLHIWIALALEYYKQGKTEEFVKLLEAARIDGNLDYRDHEKDQMTCLDTLAAYYVQQARKEKNKDNKKDLITQATLLYTMADKIIMYDQNHLLGRACFCLLEGDKMDQADAQFHFVLNQSPNNIPALLGKACISFNKKDYRGALAYYKKALRTNPGCPAEVRLGMGHCFVKLNKLEKARLAFSRALELNSKCVGALVGLAVLELNNKEADSIKNGVQLLSRAYTIDPSNPMVLNHLANHFFFKKDYSKVQHLALHAFHNTEVEAMQAESCYQLARSFHVQEDYDQAFQYYYQATQFASSSFVLPFFGLGQMYIYRGDKENASQCFEKVLKAYPNNYETMKILGSLYAASEDQEKRDIAKGHLKKVTEQYPDDVEAWIELAQILEQTDIQGALSAYGTATRILQEKVQADVPPEILNNVGALHFRLGNLGEAKKYFLASLDRAKAEAEHDEHYYNAISVTTSYNLARLYEAMCEFHEAEKLYKNILREHPNYVDCYLRLGAMARDKGNFYEASDWFKEALQINQDHPDAWSLIGNLHLAKQEWGPGQKKFERILKQPSTQSDTYSMLALGNVWLQTLHQPTRDREKEKRHQDRALAIYKQVLRNDAKNLYAANGIGAVLAHKGYFREARDVFAQVREATADISDVWLNLAHIYVEQKQYISAVQMYENCLRKFYKHQNTEVVLYLARALFKCGKLQECKQTLLKARHVAPSDTVLMFNVALVLQRLATSVLKDEKSNLKEVLNAVKELELAHRYFSYLSKVGDKMRFDLALAATEARQCSDLLSQAQYHVARARKQDEEERELRAKQEQEKELLRQKLLKEQEEKRLREKEEQKKLLEQRAQYVEKTKNILMFT

Source Source

Source organism organism	Homo sapiens
Common name common_name	human
NCBI taxonomy ID ncbi_taxonomy_id	9606
Host organism host_organism	Trichoplusia ni

Entity 18 Entity 18 polymer

Description description	RNA polymerase-associated protein RTF1 homolog
Molecular weight formula_weight	29128.2 kDa
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	R

VSLPEELNRVRLSRHKLERWCHMPFFAKTVTGCFVRIGIGNHNSKPVYRVAEITGVVETAKVYQLGGTRTNKGLQLRHGNDQRVFRLEFVSNQEFTESEFMKWKEAMFSAGMQLPTLDEINKKELSIKEALNYKFNDQDIEEIVKEKERFRKAPPNYAMKKTQLLKEKAMAEDLGDQDKAKQIQDQLNELEERAEALDRQRTKNISAISYINQRNREWNIVESEKALVAESHNMKNQQMDPFTRRQCK

Source Source

Source organism organism	Homo sapiens
Common name common_name	human
NCBI taxonomy ID ncbi_taxonomy_id	9606
Host organism host_organism	Trichoplusia ni

Entity 19 Entity 19 polymer

Description description	Transcription elongation factor A protein 1
Molecular weight formula_weight	19371.1 kDa
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	S

RAPSTSDSVRLKCREMLAAALRTGDDYIAIGADEEELGSQIEEAIYQEIRNTDMKYKNRVRSRISNLKDAKNPNLRKNVLCGNIPPDLFARMTAEEMASDELKEMRKNLTKEAIREHQMAKTGGTQTDLFTCGKCKKKNCTYTQVQTRSADEPMTTFVVCNECGNRWKFC

Source Source

Source organism organism	Homo sapiens
Common name common_name	human
NCBI taxonomy ID ncbi_taxonomy_id	9606
Host organism host_organism	Escherichia coli

Entity 20 Entity 20 polymer

Description description	Template DNA
Molecular weight formula_weight	56053.8 kDa
Source method src_method	syn
Copy count entity_count	1

Polymer Polymer

Polymer type type	polydeoxyribonucleotide
Chain IDs strand_id	T

CAAACACACCAAACACAAGAGCTAATTGACTGGCCGTTATCGATGTATATATCTGACACGTGCCTGGAGACTAGGGAGTAATCCCCTTGGCGGTTAAAACGCGGGGGACAGCGCGTACGTGCGTTTAAGCGGTGCTAGAGCTGTCTACGACCAATTGAGCGGCCTCGGCACCGGGATTCTG

Entity 21 Entity 21 polymer

Description description	RNA polymerase-associated protein LEO1
Molecular weight formula_weight	14451.3 kDa
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	U

GNDLYFVKLPNFLSVEPRPFDPQYYEDEFEEEGRTRLKLKVENNARIVKWSDGSMSLHLGNEVFDVYKAPLQGDHNHLFIRQGTGLQGQAVFKTKLTFRPHSTDSATHRKMTLSLADRCSKTQKI

Source Source

Source organism organism	Homo sapiens
NCBI taxonomy ID ncbi_taxonomy_id	9606
Host organism host_organism	Trichoplusia ni

Entity 22 Entity 22 polymer

Description description	RNA polymerase II-associated factor 1 homolog
Molecular weight formula_weight	28991.5 kDa
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	V

ERSGVVCRVKYCNSLPDIPFDPKFITYPFDQNRFVQYKATSLEKQHKHDLLTEPDLGVTIDLINPDTYRIDPNVLLDPADEKLLEEEIQRSQQHAKVVPWMRKTEYISTEFNKDRDSQITAIEKTFEDAQKSISQHYSKPRVTPVEVMPVSQAMIRGMMDEEGNQFVAYFLPVEETLKKRKRDQEEEMDYAPDDVYDYKIAREYNWNVKNKASKGYEENYFFIFREGDGVYYNELETRVRLSKR

Source Source

Source organism organism	Homo sapiens
NCBI taxonomy ID ncbi_taxonomy_id	9606
Host organism host_organism	Trichoplusia ni

Entity 23 Entity 23 polymer

Description description	Superkiller complex protein 8, N-terminally processed
Molecular weight formula_weight	33029.8 kDa
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	W

YGILFKQEQAHDDAIWSVAWGTNKKENSETVVTGSLDDLVKVWKWRDERLDLQWSLEGHQLGVVSVDISHTLPIAASSSLDAHIRLWDLENGKQIKSIDAGPVDAWTLAFSPDSQYLATGTHVGKVNIFGVESGKKEYSLDTRGKFILSIAYSPDGKYLASGAIDGIINIFDIATGKLLHTLEGHAMPIRSLTFSPDSQLLVTASDDGYIKIYDVQHANLAGTLSGHASWVLNVAFCPDDTHFVSSSSDKSVKVWDVGTRTCVHTFFDHQDQVWGVKYNGNGSKIVSVGDDQEIHIYDCP

Source Source

Source organism organism	Homo sapiens
Common name common_name	human
NCBI taxonomy ID ncbi_taxonomy_id	9606
Host organism host_organism	Trichoplusia ni

Entity 24 Entity 24 polymer

Description description	Parafibromin
Molecular weight formula_weight	5029.8 kDa
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	X

VDVTRDIVSRERVWRTRTTILQSTGKNFSKNIFAILQSVKARE

Source Source

Source organism organism	Homo sapiens
Common name common_name	human
NCBI taxonomy ID ncbi_taxonomy_id	9606
Host organism host_organism	Trichoplusia ni

Entity 25 Entity 25 polymer

Description description	Transcription elongation factor SPT4
Molecular weight formula_weight	13079.0 kDa
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	Y

ALETVPKDLRHLRACLLCSLVKTIDQFEYDGCDNCDAYLQMKGNREMVYDCTSSSFDGIIAMMSPEDSWVSKWQRVSNFKPGVYAVSVTGRLPQGIVRELKSRGVAYKSRDTAIKT

Source Source

Source organism organism	Homo sapiens
Common name common_name	human
NCBI taxonomy ID ncbi_taxonomy_id	9606
Host organism host_organism	Escherichia coli

Entity 26 Entity 26 polymer

Description description	Transcription elongation factor SPT5
Molecular weight formula_weight	57815.3 kDa
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	Z

DPNLWTVKCKIGEERATAISLMRKFIAYQFTDTPLQIKSVVAPEHVKGYIYVEAYKQTHVKQAIEGVGNLRLGYWNQQMVPIKEMTDVLKVVKANLKPKSWVRLKRGIYKDDIAQVDYVEPSQNTISLKMIPRIDYDRIKAPPQRLFDAEKIRSLGGDVASDGDFLIFEGNRYSRKGFLFKSFAMSAVITEGVKPTLSELEKFREHNFQPGDNVEVCEGELINLQGKILSVDGNKITIMPKHEDLKDMLEFPAQELRKYFKMGDHVKVIAGRFEGDTGLIVRVEENFVILFSDLTMHELKVLPRDLQLCSETASGVDVGGQHEWGELVQLDPQTVGVIVRLERETFQVLNMYGKVVTVRHQAVTRKKDNRFAVALDSEQNNIHVKDIVKVIDGPHSGREGEIRHLFRSFAFLHCKKLVENGGMFVCKTRHLVLANELIGQTVRISQGPYKGYIGVVKDATESTARVELHSTCQTISVDRQRLTTVGSRRPGGMTSTYGRYGSQTPMYGSG

Source Source

Source organism organism	Homo sapiens
NCBI taxonomy ID ncbi_taxonomy_id	9606
Host organism host_organism	Escherichia coli

Entity 27 Entity 27 polymer

Description description	Histone H3
Molecular weight formula_weight	15437.1 kDa
Source method src_method	man
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	a,e

MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVMKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVMALQEASEAYLVALFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA

Source Source

Source organism organism	Xenopus laevis
Common name common_name	African clawed frog
NCBI taxonomy ID ncbi_taxonomy_id	8355
Host organism host_organism	Escherichia coli

Entity 28 Entity 28 polymer

Description description	Histone H4
Molecular weight formula_weight	8853.3 kDa
Source method src_method	man
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	b,f

DNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKVFLENVIRDAVTYTEHAKRKTVTAMDVVYALKRQGRTLYGFG

Source Source

Source organism organism	Xenopus laevis
Common name common_name	African clawed frog
NCBI taxonomy ID ncbi_taxonomy_id	8355
Host organism host_organism	Escherichia coli

Entity 29 Entity 29 polymer

Description description	Histone H2A type 1
Molecular weight formula_weight	14109.4 kDa
Source method src_method	man
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	c,g

MSGRGKQGGKTRAKAKTRSSRAGLQFPVGRVHRLLRKGNYAERVGAGAPVYLAAVLEYLTAEILELAGNAARDNKKTRIIPRHLQLAVRNDEELNKLLGRVTIAQGGVLPNIQSVLLPKKTESSKSAKSK

Source Source

Source organism organism	Xenopus laevis
Common name common_name	African clawed frog
NCBI taxonomy ID ncbi_taxonomy_id	8355
Host organism host_organism	Escherichia coli

Entity 30 Entity 30 polymer

Description description	Histone H2B 1.1
Molecular weight formula_weight	13655.9 kDa
Source method src_method	man
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	d,h

MAKSAPAPKKGSKKAVTKTQKKDGKKRRKTRKESYAIYVYKVLKQVHPDTGISSKAMSIMNSFVNDVFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSAK

Source Source

Source organism organism	Xenopus laevis
Common name common_name	African clawed frog
NCBI taxonomy ID ncbi_taxonomy_id	8355
Host organism host_organism	Escherichia coli

Entity 31 Entity 31 polymer

Description description	Histone-lysine N-methyltransferase SETD2
Molecular weight formula_weight	67378.5 kDa
EC 编号 ec_number	2.1.1.359,2.1.1.-
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	l

DDFRDPQRWKECAKQGKMPCYFDLIEENVYLTERKKNKSHRDIKRMQCECTPLSKDERAQGEIACGEDCLNRLLMIECSSRCPNGDYCSNRRFQRKQHADVEVILTEKKGWGLRAAKDLPSNTFVLEYCGEVLDHKEFKARVKEYARNKNIHYYFMALKNDEIIDATQKGNCSRFMNHSCEPNCETQKWTVNGQLRVGFFTTKLVPSGSELTFDYQFQRYGKEAQKCFCGSANCRGYLGGENRVSIRAAGGKMKKERSRKKDSVDGELEALMENGEGLSDKNQVLSLSRLMVRIETLEQKLTCLELIQNTHSQSCLKSFLERHGLSLLWIWMAELGDGRESNQKLQEEIIKTLEHLPIPTKNMLEESKVLPIIQRWSQTKTAVPPLSEGDGYSSENTSRAHTPLNTPDPSTKLSTEADTDTPKKLMFRRLKIISENSMDSAISDATSELEGKDGKEDLDQLENVPVEEEEELQSQQLLPQQLPECKVDSETNIEASKLPTSEPEADAEIEPKESNGTKLEEPINEETPSQDEEEGVSDVESERSQEQPDKTVDISDLATKLLDSWKDLKEVYRIPKKSQTEKENTTTER

Source Source

Source organism organism	Homo sapiens
Common name common_name	human
NCBI taxonomy ID ncbi_taxonomy_id	9606
Host organism host_organism	Trichoplusia ni

Entity 32 Entity 32 non-polymer

Description description	ZINC ION
Molecular weight formula_weight	65.4 kDa
Source method src_method	syn
Copy count entity_count	9

Entity 33 Entity 33 non-polymer

Description description	MAGNESIUM ION
Molecular weight formula_weight	24.3 kDa
Source method src_method	syn
Copy count entity_count	1

▶

Unit-cell length a length_a	1.000 Å
Unit-cell length b length_b	1.000 Å
Unit-cell length c length_c	1.000 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	—

Space group space_group	P 1
Space-group number int_tables_number	1
Crystal system crystal_system	—

Resolution resolution	3.100 Å
Low-resolution limit resolution_low	— Å
R factor r_factor	—
R_work r_work	—
R_free r_free	—

Resolution resolution	3.10 Å
Resolution测定Method resolution_method	—
颗粒数 num_particles	121657

RNA polymerase II-DSIF-SPT6-PAF1c-TFIIS-IWS1-SETD2-nucleosome, 20 bp upstream

1. Protein Identity and Related Structures Protein Identity & Related Structures

DNA-directed RNA polymerase subunit

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

DNA-directed RNA polymerase subunit beta

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

DNA-directed RNA polymerase II subunit RPB3

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

RNA polymerase Rpb4/RPC9 core domain-containing protein

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

DNA-directed RNA polymerase II subunit E

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

DNA-directed RNA polymerases I, II, and III subunit RPABC2

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

DNA-directed RNA polymerase subunit

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

DNA-directed RNA polymerases I, II, and III subunit RPABC3

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

DNA-directed RNA polymerase II subunit RPB9

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

DNA-directed RNA polymerase II subunit RPB11-a

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer