Current Protein Identity：P83876
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1PQN dominant negative human hDim1 (hDim1 1-128) Deposited 2003-06-18 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
2–128（127 aa）
Fragment：dominant negative human hDim1
|
Mutation：deltaC14 | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 7.65；310 K；Pressure ambient
NMR sample composition
1mM hDim1 1-128,U-15N; 10mM phosphate buffer; 95% H2O, 5% D2O, 0.2% 2,2-dimethyl-2-silapentane-5-sulfonate
(DSS), 0.04% NaN3 | 95% H2O/5% D2O
NMR sample composition
1mM hDim1 1-128,U-15N,13C; 10mM phosphate buffer; 95% H2O, 5% D2O, 0.2% 2,2-dimethyl-2-silapentane-5-sulfonate
(DSS), 0.04% NaN3 | 95% H2O/5% D2O
NMR sample composition
1mM hDim1 1-128,U-13C; 10mM phosphate buffer; 100% D2O, 0.2% 2,2-dimethyl-2-silapentane-5-sulfonate
(DSS), 0.04% NaN3 | 100% D2O
|
Resolution not provided |
| 1QGV HUMAN SPLICEOSOMAL PROTEIN U5-15KD Deposited 1999-05-06 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1–142（142 aa）
Fragment：FULL-LENGTH
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.6；294 K；1.0 M AMMONIUM SULPHATE 0.2 M HEPES/NAOH, PH 7.6 AT 294 K, VAPOR DIFFUSION, HANGING DROP
|
Resolution 1.40 Å R-free 0.269 |
| 1SYX The crystal structure of a binary U5 snRNP complex Deposited 2004-04-02 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–142（142 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；277 K；PEG 2000mme, MES, calcium acetate, 1,4-butanediol, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
Resolution 2.35 Å R-free 0.262 |
| 1SYX The crystal structure of a binary U5 snRNP complex Deposited 2004-04-02 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain C
1–142（142 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；277 K；PEG 2000mme, MES, calcium acetate, 1,4-butanediol, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
Resolution 2.35 Å R-free 0.262 |
| 1SYX The crystal structure of a binary U5 snRNP complex Deposited 2004-04-02 | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain E
1–142（142 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；277 K；PEG 2000mme, MES, calcium acetate, 1,4-butanediol, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
Resolution 2.35 Å R-free 0.262 |
| 3JCR 3D structure determination of the human*U4/U6.U5* tri-snRNP complex Deposited 2016-01-21 | Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 36-meric（36） Consistent with all polymers |
Chain E
1–142（142 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
20 mM HEPES, pH 7.9, 100 mM KCl, 5 mM MgCl2, 0.1 mM EDTA；pH 7.9；20 mM HEPES, pH 7.9, 100 mM KCl, 5 mM MgCl2, 0.1 mM EDTA
cryo-EM vitrification conditions
Cryogen ETHANE；Plunged into liquid ethane (FEI VITROBOT MARK IV).
|
Resolution 7.00 Å |
| 4BWQ Crystal structure of U5-15kD in a complex with PQBP1 Deposited 2013-07-04 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain E
4–137（134 aa）
Fragment：RESIDUES 4-137
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.10 Å R-free 0.252 |
| 4BWQ Crystal structure of U5-15kD in a complex with PQBP1 Deposited 2013-07-04 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain G
4–137（134 aa）
Fragment：RESIDUES 4-137
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.10 Å R-free 0.252 |
| 4BWQ Crystal structure of U5-15kD in a complex with PQBP1 Deposited 2013-07-04 | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain C
4–137（134 aa）
Fragment：RESIDUES 4-137
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.10 Å R-free 0.252 |
| 4BWQ Crystal structure of U5-15kD in a complex with PQBP1 Deposited 2013-07-04 | Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
4–137（134 aa）
Fragment：RESIDUES 4-137
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.10 Å R-free 0.252 |
| 4BWS Crystal structure of the heterotrimer of PQBP1, U5-15kD and U5-52kD. Deposited 2013-07-04 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
4–137（134 aa）
Fragment：RESIDUES 4-137
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.50 Å R-free 0.277 |
| 4BWS Crystal structure of the heterotrimer of PQBP1, U5-15kD and U5-52kD. Deposited 2013-07-04 | Assembly 2 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain D
4–137（134 aa）
Fragment：RESIDUES 4-137
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.50 Å R-free 0.277 |
| 4CDO Crystal structure of PQBP1 bound to spliceosomal U5-15kD Deposited 2013-11-05 | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain C
4–137（134 aa）
Fragment：RESIDUES 4-137,223-265
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.50 Å R-free 0.238 |
| 4CDO Crystal structure of PQBP1 bound to spliceosomal U5-15kD Deposited 2013-11-05 | Assembly 2 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
4–137（134 aa）
Fragment：RESIDUES 4-137,223-265
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.50 Å R-free 0.238 |
| 5O9Z Cryo-EM structure of a pre-catalytic human spliceosome primed for activation (B complex) Deposited 2017-06-20 | Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain J
1–142（142 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.50 Å |
| 6AH0 The Cryo-EM Structure of the Precusor of Human Pre-catalytic Spliceosome (pre-B complex) Deposited 2018-08-15 | Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain O
1–142（142 aa）
|
Not recorded | IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.70 Å |
| 6AHD The Cryo-EM Structure of Human Pre-catalytic Spliceosome (B complex) at 3.8 angstrom resolution Deposited 2018-08-17 | Assembly 1 Protein–RNA Heteromer；Protein × 58 PDB declaration: 63-meric（63） Consistent with all polymers |
Chain O
1–142（142 aa）
|
Not recorded | IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 6QW6 Structure of the human U5.U4/U6 tri-snRNP at 2.9A resolution. Deposited 2019-03-05 | Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric（39） Consistent with all polymers |
Chain 5D
1–142（142 aa）
|
Not recorded | IHP INOSITOL HEXAKISPHOSPHATE × 1 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE；Wait 30s, blot for 2s to 3s.
|
Resolution 2.92 Å |
| 6QX9 Structure of a human fully-assembled precatalytic spliceosome (pre-B complex). Deposited 2019-03-07 | Assembly 1 Protein–RNA Heteromer；Protein × 65 PDB declaration: 71-meric（71） Consistent with all polymers |
Chain 5D
1–142（142 aa）
|
Not recorded | ZN ZINC ION × 6 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 IHP INOSITOL HEXAKISPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.28 Å |
| 8H6E Cryo-EM structure of human exon-defined spliceosome in the late pre-B state. Deposited 2022-10-17 | Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric（61） Consistent with all polymers |
Chain 4F
1–142（142 aa）
|
Not recorded | IHP INOSITOL HEXAKISPHOSPHATE × 1 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 8H6J Cryo-EM structure of human exon-defined spliceosome in the mature pre-B state. Deposited 2022-10-18 | Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric（61） Consistent with all polymers |
Chain 4F
1–142（142 aa）
|
Not recorded | IHP INOSITOL HEXAKISPHOSPHATE × 1 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.25 Å |
| 8H6K Cryo-EM structure of human exon-defined spliceosome in the mature B state. Deposited 2022-10-18 | Assembly 1 Protein–RNA Heteromer；Protein × 58 PDB declaration: 63-meric（63） Consistent with all polymers |
Chain 4F
1–142（142 aa）
|
Not recorded | IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.70 Å |
| 8H6L Cryo-EM structure of human exon-defined spliceosome in the early B state. Deposited 2022-10-18 | Assembly 1 Protein–RNA Heteromer；Protein × 54 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain 4F
1–142（142 aa）
|
Not recorded | IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.60 Å |
| 8Q7N cryo-EM structure of the human spliceosomal B complex protomer (tri-snRNP core region) Deposited 2023-08-16 | Assembly 1 Protein–RNA Heteromer；Protein × 17 PDB declaration: 21-meric（21） Consistent with all polymers |
Chain D
1–142（142 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 8QO9 Cryo-EM structure of a human spliceosomal B complex protomer Deposited 2023-09-28 | Assembly 1 Protein–RNA Heteromer；Protein × 66 PDB declaration: 71-meric（71） Consistent with all polymers |
Chain D
1–142（142 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.29 Å |
| 8QOZ Cryo-EM Structure of Pre-B+5'ss+ATPgammaS Complex (core part) Deposited 2023-09-29 | Assembly 1 Protein–RNA Heteromer；Protein × 13 PDB declaration: 17-meric（17） Consistent with all polymers |
Chain D
1–142（142 aa）
|
Not recorded | IHP INOSITOL HEXAKISPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 8QP8 Cryo-EM Structure of Pre-B Complex (core part) Deposited 2023-09-30 | Assembly 1 Protein–RNA Heteromer；Protein × 12 PDB declaration: 15-meric（15） Consistent with all polymers |
Chain D
1–142（142 aa）
|
Not recorded | IHP INOSITOL HEXAKISPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 8QP9 Cryo-EM Structure of Pre-B+AMPPNP Complex (core part) Deposited 2023-09-30 | Assembly 1 Protein–RNA Heteromer；Protein × 13 PDB declaration: 16-meric（16） Consistent with all polymers |
Chain D
1–142（142 aa）
|
Not recorded | IHP INOSITOL HEXAKISPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.10 Å |
| 8QPA Cryo-EM Structure of Pre-B+5'ssLNG Complex (core part) Deposited 2023-10-01 | Assembly 1 Protein–RNA Heteromer；Protein × 13 PDB declaration: heptadecameric（17） Consistent with all polymers |
Chain D
1–142（142 aa）
|
Not recorded | IHP INOSITOL HEXAKISPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 8QPB Cryo-EM Structure of Pre-B+ATP Complex (core part) Deposited 2023-10-01 | Assembly 1 Protein–RNA Heteromer；Protein × 13 PDB declaration: 17-meric（17） Consistent with all polymers |
Chain D
1–142（142 aa）
|
Not recorded | IHP INOSITOL HEXAKISPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 8QPE Cryo-EM Structure of Pre-B-like Complex (core part) Deposited 2023-10-01 | Assembly 1 Protein–RNA Heteromer；Protein × 15 PDB declaration: 20-meric（20） Consistent with all polymers |
Chain D
1–142（142 aa）
|
Not recorded | IHP INOSITOL HEXAKISPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 8QPK Cryo-EM Structure of Pre-B+5'ss Complex (core part) Deposited 2023-10-02 | Assembly 1 Protein–RNA Heteromer；Protein × 12 PDB declaration: hexadecameric（16） Consistent with all polymers |
Chain D
1–142（142 aa）
|
Not recorded | IHP INOSITOL HEXAKISPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.20 Å |
| 8QXD Cryo-EM structure of the cross-exon pre-B complex Deposited 2023-10-24 | Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric（61） Consistent with all polymers |
Chain D
1–142（142 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 9.60 Å |
| 8QZS Cryo-EM structure of the cross-exon B-like complex Deposited 2023-10-29 | Assembly 1 Protein–RNA Heteromer；Protein × 61 PDB declaration: 68-meric（68） Consistent with all polymers |
Chain D
1–142（142 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.10 Å |
| 8R08 Cryo-EM structure of the cross-exon pre-B+AMPPNP complex Deposited 2023-10-31 | Assembly 1 Protein–RNA Heteromer；Protein × 64 PDB declaration: 71-meric（71） Consistent with all polymers |
Chain D
1–142（142 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.10 Å |
| 8R09 Cryo-EM structure of the cross-exon pre-B+5'ss+ATPgammaS complex Deposited 2023-10-31 | Assembly 1 Protein–RNA Heteromer；Protein × 54 PDB declaration: 60-meric（60） Consistent with all polymers |
Chain D
1–142（142 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.30 Å |
| 8R0A Cryo-EM structure of the cross-exon pre-B+5'ss complex Deposited 2023-10-31 | Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 62-meric（62） Consistent with all polymers |
Chain D
1–142（142 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.80 Å |
| 8R0B Cryo-EM structure of the cross-exon pre-B+ATP complex Deposited 2023-10-31 | Assembly 1 Protein–RNA Heteromer；Protein × 54 PDB declaration: 60-meric（60） Consistent with all polymers |
Chain D
1–142（142 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.40 Å |
| 8RM5 Cryo-EM structure of the cross-exon pre-B+5'ssLNG+ATPyS complex Deposited 2024-01-05 | Assembly 1 Protein–RNA Heteromer；Protein × 54 PDB declaration: 60-meric（60） Consistent with all polymers |
Chain D
1–142（142 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.90 Å |