当前蛋白身份：Q02368
重新检索
差异标签只比较当前检索结果；所有PDB和assembly原始记录仍分别保留。
相关结构差异明细
一行代表一个 PDB 条目中的一个 biological assembly；同一蛋白的多个单体会分别列出。
| PDB 条目 | Assembly / 聚集状态 | 构建体 | 突变与修饰 | 配体、离子与非聚合物 | 实验方法 | 实验环境 | 结构质量 |
|---|---|---|---|---|---|---|---|
| 5LC5 Structure of mammalian respiratory Complex I, class2 提交 2016-06-19 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 45 PDB 声明：45-meric（45） 与蛋白数一致 |
链 o
58–115（58 aa）
|
未记录 | SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 8
cryo-EM玻璃化条件
冷冻剂 ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
|
分辨率 4.35 Å |
| 5LDW Structure of mammalian respiratory Complex I, class1 提交 2016-06-28 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 45 PDB 声明：45-meric（45） 与蛋白数一致 |
链 o
2–137（136 aa）
|
未记录 | SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 8；20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7
cryo-EM玻璃化条件
冷冻剂 ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus present in a cold room, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
|
分辨率 4.27 Å |
| 5LDX Structure of mammalian respiratory Complex I, class3. 提交 2016-06-28 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 45 PDB 声明：45-meric（45） 与蛋白数一致 |
链 o
58–115（58 aa）
|
未记录 | SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7；pH 8
cryo-EM玻璃化条件
冷冻剂 ETHANE；The specimen was vitrified in an environmental plunge-freeze apparatus, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged.
|
分辨率 5.60 Å |
| 5O31 Mitochondrial complex I in the deactive state 提交 2017-05-23 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 45 PDB 声明：45-meric（45） 与蛋白数一致 |
链 o
2–137（136 aa）
|
未记录 | SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.55
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 4.13 Å |
| 7DGQ Activity optimized supercomplex state1 提交 2020-11-12 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 80 PDB 声明：80-meric（80） 与蛋白数一致 |
链 d
2–118（117 aa）
|
未记录 | PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 CU COPPER (II) ION × 3 HEA HEME-A × 2 MG MAGNESIUM ION × 1 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 UQ2 UBIQUINONE-2 × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.4
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 5.00 Å |
| 7DGR Activity optimized supercomplex state2 提交 2020-11-12 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 80 PDB 声明：80-meric（80） 与蛋白数一致 |
链 d
2–118（117 aa）
|
未记录 | FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 3 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.4
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 4.60 Å |
| 7DGS Activity optimized supercomplex state3 提交 2020-11-12 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 80 PDB 声明：80-meric（80） 与蛋白数一致 |
链 d
2–118（117 aa）
|
未记录 | FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.4
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 7.80 Å |
| 7DGZ Activity optimized complex I (closed form) 提交 2020-11-12 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 45 PDB 声明：45-meric（45） 与蛋白数一致 |
链 d
2–137（136 aa）
|
未记录 | 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 2 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.4
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.80 Å |
| 7DH0 Activity optimized complex I (open form) 提交 2020-11-12 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 45 PDB 声明：45-meric（45） 与蛋白数一致 |
链 d
2–137（136 aa）
|
未记录 | 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.4
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 4.20 Å |
| 7DKF Activity optimized supercomplex state4 提交 2020-11-24 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 80 PDB 声明：80-meric（80） 与蛋白数一致 |
链 d2
2–137（136 aa）
|
未记录 | HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.4
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 8.30 Å |
| 7QSD Bovine complex I in the active state at 3.1 A 提交 2022-01-13 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 45 PDB 声明：45-meric（45） 与蛋白数一致 |
链 o
1–137（137 aa）
|
未记录 | PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 CDL CARDIOLIPIN × 4 LMT DODECYL-BETA-D-MALTOSIDE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.14
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.10 Å |
| 7QSK Bovine complex I in lipid nanodisc, Active-Q10 提交 2022-01-13 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 45 PDB 声明：45-meric（45） 与蛋白数一致 |
链 o
1–137（137 aa）
|
未记录 | 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE；blot for 10 seconds before plunging
|
分辨率 2.84 Å |
| 7QSL Bovine complex I in lipid nanodisc, Active-apo 提交 2022-01-13 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 45 PDB 声明：45-meric（45） 与蛋白数一致 |
链 o
1–137（137 aa）
|
未记录 | 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE；blot for 10 seconds before plunging
|
分辨率 2.76 Å |
| 7QSM Bovine complex I in lipid nanodisc, Deactive-ligand (composite) 提交 2022-01-13 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 45 PDB 声明：45-meric（45） 与蛋白数一致 |
链 o
1–137（137 aa）
|
未记录 | 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 GOL GLYCEROL × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE；blot for 10 seconds before plunging
|
分辨率 2.30 Å |
| 7QSN Bovine complex I in lipid nanodisc, Deactive-apo 提交 2022-01-13 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 45 PDB 声明：45-meric（45） 与蛋白数一致 |
链 o
1–137（137 aa）
|
未记录 | PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE；blot for 10 seconds before plunging
|
分辨率 2.81 Å |
| 7QSO Bovine complex I in lipid nanodisc, State 3 (Slack) 提交 2022-01-13 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 45 PDB 声明：45-meric（45） 与蛋白数一致 |
链 o
1–137（137 aa）
|
未记录 | 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 6 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 2 CDL CARDIOLIPIN × 6 U10 UBIQUINONE-10 × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE；blot for 10 seconds before plunging
|
分辨率 3.02 Å |
| 7R41 Bovine complex I in the presence of IM1761092, active class i (Composite map) 提交 2022-02-08 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 45 PDB 声明：45-meric（45） 与蛋白数一致 |
链 o
1–137（137 aa）
|
未记录 | LMT DODECYL-BETA-D-MALTOSIDE × 16 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.14
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 2.30 Å |
| 7R42 Bovine complex I in the presence of IM1761092, active class ii (Composite map) 提交 2022-02-08 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 45 PDB 声明：45-meric（45） 与蛋白数一致 |
链 o
1–137（137 aa）
|
未记录 | LMT DODECYL-BETA-D-MALTOSIDE × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.14
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 2.30 Å |
| 7R43 Bovine complex I in the presence of IM1761092, active class iii (Composite map) 提交 2022-02-08 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 45 PDB 声明：45-meric（45） 与蛋白数一致 |
链 o
1–137（137 aa）
|
未记录 | LMT DODECYL-BETA-D-MALTOSIDE × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 CDL CARDIOLIPIN × 5 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.14
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 2.40 Å |
| 7R44 Bovine complex I in the presence of IM1761092, active class iv (Composite map) 提交 2022-02-08 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 45 PDB 声明：45-meric（45） 与蛋白数一致 |
链 o
1–137（137 aa）
|
未记录 | LMT DODECYL-BETA-D-MALTOSIDE × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.14
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 2.40 Å |
| 7R45 Bovine complex I in the presence of IM1761092, deactive class i (Composite map) 提交 2022-02-08 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 45 PDB 声明：45-meric（45） 与蛋白数一致 |
链 o
1–137（137 aa）
|
未记录 | LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.14
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 2.40 Å |
| 7R46 Bovine complex I in the presence of IM1761092, deactive class ii (Composite map) 提交 2022-02-08 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 45 PDB 声明：45-meric（45） 与蛋白数一致 |
链 o
1–137（137 aa）
|
未记录 | LMT DODECYL-BETA-D-MALTOSIDE × 11 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 14 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.14
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 2.40 Å |
| 7R47 Bovine complex I in the presence of IM1761092, deactive class iii (Composite map) 提交 2022-02-08 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 45 PDB 声明：45-meric（45） 与蛋白数一致 |
链 o
1–137（137 aa）
|
未记录 | LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.14
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 2.30 Å |
| 7R48 Bovine complex I in the presence of IM1761092, deactive class iv (Composite map) 提交 2022-02-08 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 45 PDB 声明：45-meric（45） 与蛋白数一致 |
链 o
2–137（136 aa）
|
未记录 | LMT DODECYL-BETA-D-MALTOSIDE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 13 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.14
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 2.30 Å |
| 7R4C Bovine complex I in the presence of IM1761092, deactive class v (Composite map) 提交 2022-02-08 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 45 PDB 声明：45-meric（45） 与蛋白数一致 |
链 o
1–137（137 aa）
|
未记录 | LMT DODECYL-BETA-D-MALTOSIDE × 12 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 12 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.14
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 2.30 Å |
| 7R4D Bovine complex I in the presence of IM1761092, deactive class vi (Composite map) 提交 2022-02-08 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 45 PDB 声明：45-meric（45） 与蛋白数一致 |
链 o
1–137（137 aa）
|
未记录 | LMT DODECYL-BETA-D-MALTOSIDE × 13 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 11 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.14
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 2.30 Å |
| 7R4F Bovine complex I in the presence of IM1761092, slack class i (Composite map) 提交 2022-02-08 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 45 PDB 声明：45-meric（45） 与蛋白数一致 |
链 o
1–137（137 aa）
|
未记录 | LMT DODECYL-BETA-D-MALTOSIDE × 7 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 3 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 CDL CARDIOLIPIN × 6 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.14
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 2.40 Å |
| 7R4G Bovine complex I in the presence of IM1761092, slack class ii (Composite map) 提交 2022-02-08 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 45 PDB 声明：45-meric（45） 与蛋白数一致 |
链 o
1–137（137 aa）
|
未记录 | 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 SF4 IRON/SULFUR CLUSTER × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 10 I49 1-carbamimidoyl-3-[2-(3-chloranyl-4-iodanyl-phenyl)ethyl]guanidine × 2 CDL CARDIOLIPIN × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.14
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 2.50 Å |
| 8Q0A Inward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. 提交 2023-07-28 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 45 PDB 声明：45-meric（45） 与蛋白数一致 |
链 o
1–137（137 aa）
|
未记录 | 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 8 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE；Sample was incubated on grid for 30 s before plunge freezing
|
分辨率 3.10 Å |
| 8Q0F Inward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. 提交 2023-07-28 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 45 PDB 声明：45-meric（45） 与蛋白数一致 |
链 o
1–137（137 aa）
|
未记录 | 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 21 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE；Sample was incubated on grid for 30 s before plunge freezing
|
分辨率 3.10 Å |
| 8Q0J Inward-facing, slack proteoliposome complex I at 3.8 A. Initially purified in DDM. 提交 2023-07-28 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 45 PDB 声明：45-meric（45） 与蛋白数一致 |
链 o
1–137（137 aa）
|
未记录 | 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE；Sample was incubated on grid for 30 s before plunge freezing
|
分辨率 3.80 Å |
| 8Q0M Outward-facing, closed proteoliposome complex I at 3.1 A. Initially purified in DDM. 提交 2023-07-28 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 45 PDB 声明：45-meric（45） 与蛋白数一致 |
链 o
1–137（137 aa）
|
未记录 | 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE；Sample was incubated on grid for 30 s before plunge freezing
|
分辨率 3.10 Å |
| 8Q0O Outward-facing, open2 proteoliposome complex I at 3.1 A. Initially purified in DDM. 提交 2023-07-28 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 45 PDB 声明：45-meric（45） 与蛋白数一致 |
链 o
1–137（137 aa）
|
未记录 | 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 28 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE；Sample was incubated on grid for 30 s before plunge freezing
|
分辨率 3.10 Å |
| 8Q0Q Outward-facing, slack proteoliposome complex I at 3.6 A. Initially purified in DDM 提交 2023-07-28 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 45 PDB 声明：45-meric（45） 与蛋白数一致 |
链 o
1–137（137 aa）
|
未记录 | 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 10 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 4 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE；Sample was incubated on grid for 30 s before plunge freezing
|
分辨率 3.60 Å |
| 8Q1P Inward-facing, open2 proteoliposome complex I at 2.9 A, after deactivation treatment. Initially purified in LMNG. 提交 2023-08-01 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 45 PDB 声明：45-meric（45） 与蛋白数一致 |
链 o
1–137（137 aa）
|
未记录 | 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE；Sample was incubated on grid for 30 s before plunge freezing
|
分辨率 2.90 Å |
| 8Q1U Inward-facing, open1 proteoliposome complex I at 3.3 A, after deactivation treatment. Initially purified in LMNG. 提交 2023-08-01 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 45 PDB 声明：45-meric（45） 与蛋白数一致 |
链 o
1–137（137 aa）
|
未记录 | 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE；Sample was incubated on grid for 30 s before plunge freezing
|
分辨率 3.30 Å |
| 8Q1Y Outward-facing, open2 proteoliposome complex I at 2.6 A, after deactivation treatment. Initially purified in LMNG. 提交 2023-08-01 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 45 PDB 声明：45-meric（45） 与蛋白数一致 |
链 o
1–137（137 aa）
|
未记录 | 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE；Sample was incubated on grid for 30 s before plunge freezing
|
分辨率 2.60 Å |
| 8Q25 Outward-facing, open1 proteoliposome complex I at 2.8 A, after deactivation treatment. Initially purified in LMNG. 提交 2023-08-01 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 45 PDB 声明：45-meric（45） 与蛋白数一致 |
链 o
1–137（137 aa）
|
未记录 | 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 ZN ZINC ION × 2 MG MAGNESIUM ION × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE；Sample was incubated on grid for 30 s before plunge freezing
|
分辨率 2.80 Å |
| 8Q45 Inward-facing, closed proteoliposome complex I at 2.7 A. Initially purified in LMNG. 提交 2023-08-05 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 45 PDB 声明：45-meric（45） 与蛋白数一致 |
链 o
1–137（137 aa）
|
未记录 | 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 16 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE；Sample was incubated on grid for 30 s before plunge freezing
|
分辨率 2.70 Å |
| 8Q46 Inward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. 提交 2023-08-05 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 45 PDB 声明：45-meric（45） 与蛋白数一致 |
链 o
1–137（137 aa）
|
未记录 | 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 24 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 7 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE；Sample was incubated on grid for 30 s before plunge freezing
|
分辨率 2.60 Å |
| 8Q47 Inward-facing, open1 proteoliposome complex I at 2.9 A. Initially purified in LMNG. 提交 2023-08-05 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 45 PDB 声明：45-meric（45） 与蛋白数一致 |
链 o
1–137（137 aa）
|
未记录 | PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 26 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE；Sample was incubated on grid for 30 s before plunge freezing
|
分辨率 2.90 Å |
| 8Q48 Outward-facing, closed proteoliposome complex I at 2.5 A. Initially purified in LMNG. 提交 2023-08-05 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 45 PDB 声明：45-meric（45） 与蛋白数一致 |
链 o
1–137（137 aa）
|
未记录 | PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 35 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 9 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE；Sample was incubated on grid for 30 s before plunge freezing
|
分辨率 2.50 Å |
| 8Q49 Outward-facing, open2 proteoliposome complex I at 2.6 A. Initially purified in LMNG. 提交 2023-08-05 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 45 PDB 声明：45-meric（45） 与蛋白数一致 |
链 o
1–137（137 aa）
|
未记录 | PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 32 SF4 IRON/SULFUR CLUSTER × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CHD CHOLIC ACID × 1 ZN ZINC ION × 2 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 FVH [[(2~{R},3~{S},4~{R},5~{R})-5-[(3~{R})-3-aminocarbonylpiperidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2~{R},3~{R},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3-oxidanyl-4-phosphonooxy-oxolan-2-yl]methyl hydrogen phosphate × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE；Sample was incubated on grid for 30 s before plunge freezing
|
分辨率 2.60 Å |
| 8Q4A Outward-facing, open1 proteoliposome complex I at 2.6 A. Initially purified in LMNG. 提交 2023-08-05 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 45 PDB 声明：45-meric（45） 与蛋白数一致 |
链 o
1–137（137 aa）
|
未记录 | 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 29 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 17 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 9 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 CDL CARDIOLIPIN × 8 ZN ZINC ION × 2 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE；Sample was incubated on grid for 30 s before plunge freezing
|
分辨率 2.60 Å |
| 9SMF Reduced bovine complex I in lipid nanodisc, NADH-active-Q10 提交 2025-09-08 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 45 PDB 声明：45-meric（45） 与蛋白数一致 |
链 o
1–137（137 aa）
|
未记录 | 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 20 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 U10 UBIQUINONE-10 × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 8 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE；Mix with NADH, then blot for 10 seconds before plunging
|
分辨率 2.51 Å |
| 9SMG Reduced bovine complex I in lipid nanodisc, NADH-active-altQ10 提交 2025-09-08 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 45 PDB 声明：45-meric（45） 与蛋白数一致 |
链 o
1–137（137 aa）
|
未记录 | PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 U10 UBIQUINONE-10 × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE；Mix with NADH, then blot for 10 seconds before plunging
|
分辨率 2.42 Å |
| 9SMH Reduced bovine complex I in lipid nanodisc, NADH-active-DDM 提交 2025-09-08 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 45 PDB 声明：45-meric（45） 与蛋白数一致 |
链 o
1–137（137 aa）
|
未记录 | 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 17 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 CDL CARDIOLIPIN × 7 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 CHD CHOLIC ACID × 1 MYR MYRISTIC ACID × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE；Mix with NADH, then blot for 10 seconds before plunging
|
分辨率 2.61 Å |
| 9SMI Reduced bovine complex I in lipid nanodisc, NADH-deactive 提交 2025-09-08 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 45 PDB 声明：45-meric（45） 与蛋白数一致 |
链 o
1–137（137 aa）
|
未记录 | 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 27 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 7 SF4 IRON/SULFUR CLUSTER × 6 LMT DODECYL-BETA-D-MALTOSIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 5 CHD CHOLIC ACID × 1 DGT 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 EHZ ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate × 2 MYR MYRISTIC ACID × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE；Mix with NADH, then blot for 10 seconds before plunging
|
分辨率 2.01 Å |
| 9W2U Cryo-EM structure of complex I on the bovine heart submitochondrial particles, open 提交 2025-07-28 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 45 PDB 声明：45-meric（45） 与蛋白数一致 |
链 o
2–123（122 aa）
|
未记录 | FME N-FORMYLMETHIONINE × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 11 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 18 CDL CARDIOLIPIN × 6 CHD CHOLIC ACID × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 MYR MYRISTIC ACID × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 2.60 Å |
| 9W2V Cryo-EM structure of complex I on the bovine heart submitochondrial particles, closed 提交 2025-07-28 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 45 PDB 声明：45-meric（45） 与蛋白数一致 |
链 o
2–123（122 aa）
|
未记录 | FME N-FORMYLMETHIONINE × 4 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 19 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 12 SF4 IRON/SULFUR CLUSTER × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 K POTASSIUM ION × 1 CDL CARDIOLIPIN × 6 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 2.80 Å |