Ribonuclease H
OrganismNot specified
State in the Current Structure
| Assembly | Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Associated Components | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|
| 1 | Protein–DNA Homooligomer Protein × 2 DNA 2 PDB declaration: tetrameric（4） Consistent with all polymer counts | Chain A； UniProt 61–194 | Mutation：D132N | ;DNA (5'-D(*DCP*DGP*DCP*DGP*DAP*DAP*DTP*DTP*DCP*DGP*DCP*DG)-3') ; × 2 | X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, SITTING DROP；pH 4.6；291 K；0.1 M sodium acetate, 8 % (w/v) PEG 4000, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 291K | Resolution 1.80 Å R-free 0.241 |
| 2 | Protein–DNA Homooligomer Protein × 2 DNA 2 PDB declaration: tetrameric（4） Consistent with all polymer counts | Chain C； UniProt 61–194 | Mutation：D132N | ;DNA (5'-D(*DCP*DGP*DCP*DGP*DAP*DAP*DTP*DTP*DCP*DGP*DCP*DG)-3') ; × 2 NA SODIUM ION × 2 | X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, SITTING DROP；pH 4.6；291 K；0.1 M sodium acetate, 8 % (w/v) PEG 4000, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 291K | Resolution 1.80 Å R-free 0.241 |
Other States of the Same Protein in the Database
Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.
| Other PDB | Difference from Current Entry 3D0P | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1ZBF Crystal structure of B. halodurans RNase H catalytic domain mutant D132N Deposited 2005-04-08 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
59–196（138 aa）
Fragment：catalytic domain (residues 59-196)
|
Mutation：D132N | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 4.6；277 K；PEG 2000 monomethyl ether, ammonium sulfate, sodium acetate, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
Resolution 1.50 Å R-free 0.214 |
| 1ZBI Bacillus halodurans RNase H catalytic domain mutant D132N in complex with 12-mer RNA/DNA hybrid Deposited 2005-04-08 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Homooligomer；Protein × 2 PDB declaration: tetrameric |
Chain A
59–196（138 aa）
Fragment：catalytic domain (residues 59-196)
Chain B
59–196（138 aa）
Fragment：catalytic domain (residues 59-196)
|
Mutation：D132N Mutation：D132N | MG MAGNESIUM ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；294 K；MPD, NaCl, HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K
|
Resolution 1.85 Å R-free 0.248 |
| 2G8F B. halodurans RNase H catalytic domain E188A mutant in complex with Mg2+ and RNA/DNA hybrid (non-P nick at the active site) Deposited 2006-03-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: trimeric |
Chain A
59–196（138 aa）
Fragment：Bh-RNase HC
|
Mutation：E188A | MG MAGNESIUM ION × 2 CL CHLORIDE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.6；298 K；30% MPD, 0.1M citrate pH 5.6, 0.25M ammonium acetate, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 1.65 Å |
| 2G8H B. halodurans RNase H catalytic domain D192N mutant in complex with Mg2+ and RNA/DNA hybrid (non-P nick at the active site) Deposited 2006-03-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: trimeric |
Chain A
59–196（138 aa）
Fragment：Bh-RNase HC
|
Mutation：D192N | MG MAGNESIUM ION × 2 VO4 VANADATE ION × 6 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；278 K；32% MPD, 0.1M Tris pH 7.0, 0.2 M NaCl, VAPOR DIFFUSION, SITTING DROP, temperature 278K
|
Resolution 1.85 Å R-free 0.254 |
| 2G8I B. halodurans RNase H catalytic domain D192N mutant in complex with Mn2+ and RNA/DNA hybrid (non-P nick at the active site) Deposited 2006-03-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: trimeric |
Chain A
59–196（138 aa）
Fragment：Bh-RNase HC
|
Mutation：D192N | MN MANGANESE (II) ION × 4 CL CHLORIDE ION × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；278 K；35% MPD, 0.1M Tris pH 7.0, 0.2 M NaCl, VAPOR DIFFUSION, SITTING DROP, temperature 278K
|
Resolution 1.65 Å R-free 0.202 |
| 2G8K B. halodurans RNase H catalytic domain D192N mutant in complex with Ca2+ and RNA/DNA hybrid (non-P nick at the active site) Deposited 2006-03-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: trimeric |
Chain A
59–196（138 aa）
Fragment：Bh-RNase HC
|
Mutation：D192N | CA CALCIUM ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.6；278 K；27% MPD, 0.25M ammonium acetate, Na Citrate pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 278K
|
Resolution 1.65 Å R-free 0.210 |
| 2G8U B. halodurans RNase H catalytic domain D132N mutant in complex with Mg2+ and RNA/DNA hybrid (non-P nick at the active site) Deposited 2006-03-03 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: trimeric |
Chain A
59–196（138 aa）
Fragment：Bh-RNase HC
|
Mutation：D132N | MG MAGNESIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；278 K；25% MPD, 0.2M NaCl, 0.1M HEPES pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 278K
|
Resolution 2.70 Å R-free 0.288 |
| 2G8V B. halodurans RNase H catalytic domain E188A mutant in complex with Mg2+ and RNA/DNA hybrid (reaction product) Deposited 2006-03-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: trimeric |
Chain A
59–196（138 aa）
Fragment：Bh-RNase HC
|
Mutation：E188A | MG MAGNESIUM ION × 2 EOH ETHANOL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；278 K；20% EtOH, 0.1 M Tris pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 278K
|
Resolution 1.85 Å R-free 0.240 |
| 2G8W B. halodurans RNase H catalytic domain E188A mutant in complex with Ca2+ and RNA/DNA hybrid Deposited 2006-03-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: trimeric |
Chain A
59–196（138 aa）
Fragment：Bh-RNase HC
|
Mutation：E188A | CA CALCIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6；278 K；12% isopropanol, 0.1M MES 6.0, 0.3M CaAc2, VAPOR DIFFUSION, SITTING DROP, temperature 278K
|
Resolution 2.05 Å R-free 0.242 |
| 2R7Y Selenium Derivatized RNA/DNA Hybrid in complex with RNase H CATALYTIC DOMAIN MUTANT D132N Deposited 2007-09-10 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: trimeric |
Chain A
62–193（132 aa）
|
Mutation：D132N | PO4 PHOSPHATE ION × 1 MG MAGNESIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；0.1 M MES, 12% PEG 20000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.80 Å R-free 0.231 |
| 3EY1 A Conformational Transition in the Structure of a 2'-Thiomethyl-Modified DNA Visualized at High Resolution Deposited 2008-10-17 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: tetrameric |
Chain A
59–196（138 aa）
Fragment：RNase H domain, UNP residues 59-196
|
Mutation：D132N | GOL GLYCEROL × 6 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 4.6；291 K；0.1 M Sodium Acetate (pH 4.6), 0.2 M ammonium sulphate, 25% PEG 4000., VAPOR DIFFUSION, SITTING DROP, temperature 291K
|
Resolution 1.60 Å R-free 0.261 |
| 3I8D The Pairing Geometry of the Hydrophobic Thymine Analog 2,4-Difluorotoluene in Duplex DNA as Analyzed by X-ray Crystallography Deposited 2009-07-09 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: tetrameric |
Chain A
62–193（132 aa）
Fragment：RNase-H
|
Mutation：D132N | GOL GLYCEROL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 4.6；291 K；0.1 M NaOAc 3H2O (pH 4.6) and 8% (w/v) PEG 4000
, VAPOR DIFFUSION, SITTING DROP, temperature 291K
|
Resolution 1.61 Å R-free 0.238 |
| 3I8D The Pairing Geometry of the Hydrophobic Thymine Analog 2,4-Difluorotoluene in Duplex DNA as Analyzed by X-ray Crystallography Deposited 2009-07-09 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein–DNA Homooligomer；Protein × 2 PDB declaration: tetrameric |
Chain C
62–193（132 aa）
Fragment：RNase-H
|
Mutation：D132N | GOL GLYCEROL × 6 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 4.6；291 K；0.1 M NaOAc 3H2O (pH 4.6) and 8% (w/v) PEG 4000
, VAPOR DIFFUSION, SITTING DROP, temperature 291K
|
Resolution 1.61 Å R-free 0.238 |
| 3TWH Selenium Derivatized RNA/DNA Hybrid in complex with RNase H Catalytic Domain D132N Mutant Deposited 2011-09-21 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: trimeric |
Chain A
59–196（138 aa）
Fragment：catalytic domain
|
Mutation：D132N | MG MAGNESIUM ION × 2 PO4 PHOSPHATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；0.1 M MES, 12% PEG 20000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.79 Å R-free 0.208 |
| 3ULD High resolution structure of DNA/RNA hybrid in complex with RNase H catalytic domain D132N mutant Deposited 2011-11-10 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: trimeric |
Chain A
59–196（138 aa）
Fragment：catalytic domain
|
Mutation：D132N | MG MAGNESIUM ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6；298 K；0.04 M Magnesium chloride, 0.05 M Sodium cacodylate, 5% MPD, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 1.60 Å R-free 0.235 |
| 4HTU Structure of 5-chlorouracil modified A:U base pair Deposited 2012-11-01 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–DNA Monomer；Protein × 1 PDB declaration: trimeric |
Chain A
61–194（134 aa）
|
Not recorded | MG MAGNESIUM ION × 1 GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；277 K；0.2 M magnesium acetate, 0.1 M sodium cacodylate (pH 6.5) and 20% (w/v) PEG 8000, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
Resolution 1.49 Å R-free 0.218 |
| 4HTU Structure of 5-chlorouracil modified A:U base pair Deposited 2012-11-01 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein–DNA Monomer；Protein × 1 PDB declaration: trimeric |
Chain B
61–194（134 aa）
|
Not recorded | MG MAGNESIUM ION × 1 GOL GLYCEROL × 2 EDO 1,2-ETHANEDIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；277 K；0.2 M magnesium acetate, 0.1 M sodium cacodylate (pH 6.5) and 20% (w/v) PEG 8000, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
Resolution 1.49 Å R-free 0.218 |
| 4HUE Structure of 5-chlorouracil modified G:U base pair Deposited 2012-11-02 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–DNA Monomer；Protein × 1 PDB declaration: trimeric |
Chain A
61–194（134 aa）
|
Mutation：D132N | GOL GLYCEROL × 3 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；277 K；0.2 M magnesium acetate, 0.1 M sodium cacodylate and 20% (w/v) PEG 8000, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
Resolution 1.56 Å R-free 0.220 |
| 4HUE Structure of 5-chlorouracil modified G:U base pair Deposited 2012-11-02 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein–DNA Monomer；Protein × 1 PDB declaration: trimeric |
Chain B
61–194（134 aa）
|
Mutation：D132N | GOL GLYCEROL × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；277 K；0.2 M magnesium acetate, 0.1 M sodium cacodylate and 20% (w/v) PEG 8000, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
Resolution 1.56 Å R-free 0.220 |
| 4HUF Structure of 5-chlorouracil modified A:U base pair Deposited 2012-11-02 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–DNA Monomer；Protein × 1 PDB declaration: trimeric |
Chain A
61–194（134 aa）
|
Mutation：D132N | GOL GLYCEROL × 2 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；0.2 M magnesium acetate, 0.1 M sodium cacodylate (pH 6.5) and 20% (w/v) PEG 8000 , VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.69 Å R-free 0.228 |
| 4HUF Structure of 5-chlorouracil modified A:U base pair Deposited 2012-11-02 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein–DNA Monomer；Protein × 1 PDB declaration: trimeric |
Chain B
61–194（134 aa）
|
Mutation：D132N | MG MAGNESIUM ION × 1 EDO 1,2-ETHANEDIOL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；0.2 M magnesium acetate, 0.1 M sodium cacodylate (pH 6.5) and 20% (w/v) PEG 8000 , VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.69 Å R-free 0.228 |
| 4HUG Structure of 5-chlorouracil modified A:U base pairs Deposited 2012-11-02 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–DNA Monomer；Protein × 1 PDB declaration: trimeric |
Chain A
61–194（134 aa）
|
Mutation：D132N | MG MAGNESIUM ION × 1 GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 6.5；277 K；0.2 M magnesium acetate, 0.1 M sodium cacodylate and 20% (w/v) PEG 8000 , pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
Resolution 1.64 Å R-free 0.222 |
| 4HUG Structure of 5-chlorouracil modified A:U base pairs Deposited 2012-11-02 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein–DNA Monomer；Protein × 1 PDB declaration: trimeric |
Chain B
61–194（134 aa）
|
Mutation：D132N | MG MAGNESIUM ION × 1 GOL GLYCEROL × 1 EDO 1,2-ETHANEDIOL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 6.5；277 K；0.2 M magnesium acetate, 0.1 M sodium cacodylate and 20% (w/v) PEG 8000 , pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
Resolution 1.64 Å R-free 0.222 |
| 4OPJ Bh-RNaseH:tcdA-DNA complex Deposited 2014-02-05 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–DNA Monomer；Protein × 1 PDB declaration: trimeric |
Chain A
59–196（138 aa）
Fragment：UNP residues 59-196
|
Mutation：D132N | GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 4.6；291 K；0.1 M sodium acetate, 10% w/v PEG4000, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 291K
|
Resolution 1.54 Å R-free 0.220 |
| 4OPJ Bh-RNaseH:tcdA-DNA complex Deposited 2014-02-05 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein–DNA Monomer；Protein × 1 PDB declaration: trimeric |
Chain C
59–196（138 aa）
Fragment：UNP residues 59-196
|
Mutation：D132N | GOL GLYCEROL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 4.6；291 K；0.1 M sodium acetate, 10% w/v PEG4000, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 291K
|
Resolution 1.54 Å R-free 0.220 |
| 4OPK Bh-RNaseH:2'-SMe-DNA complex Deposited 2014-02-05 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–DNA Monomer；Protein × 1 PDB declaration: trimeric |
Chain A
59–196（138 aa）
Fragment：UNP residues 59-196
|
Mutation：D132N | GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 4.6；291 K；0.1 M sodium acetate, 8% w/v PEG4000, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 291K
|
Resolution 1.54 Å R-free 0.258 |
| 4OPK Bh-RNaseH:2'-SMe-DNA complex Deposited 2014-02-05 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein–DNA Monomer；Protein × 1 PDB declaration: trimeric |
Chain C
59–196（138 aa）
Fragment：UNP residues 59-196
|
Mutation：D132N | GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 4.6；291 K；0.1 M sodium acetate, 8% w/v PEG4000, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 291K
|
Resolution 1.54 Å R-free 0.258 |
| 5SWM BACILLUS HALODURANS RNASE H MUTANT D132N IN COMPLEX WITH 12-MER FRNA/DNA HYBRID Deposited 2016-08-08 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: trimeric |
Chain A
59–196（138 aa）
|
Mutation：D132N | NA SODIUM ION × 1 CL CHLORIDE ION × 1 PEG DI(HYDROXYETHYL)ETHER × 1 GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；291 K；0.4 M ammonium phosphate monobasic
|
Resolution 1.50 Å R-free 0.185 |
| 5SWM BACILLUS HALODURANS RNASE H MUTANT D132N IN COMPLEX WITH 12-MER FRNA/DNA HYBRID Deposited 2016-08-08 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Other combination Monomer；Protein × 1 PDB declaration: trimeric |
Chain B
59–196（138 aa）
|
Mutation：D132N | GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；291 K；0.4 M ammonium phosphate monobasic
|
Resolution 1.50 Å R-free 0.185 |
| 5US2 2-Se-T2-DNA and native RNA hybrid in complex with RNase H catalytic domain D132N mutant Deposited 2017-02-13 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: trimeric |
Chain A
59–196（138 aa）
Fragment：residues 59-196
|
Mutation：D132N | MG MAGNESIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；0.1M MES pH 6.5, 12%(w/v) PEG 20000
|
Resolution 1.90 Å R-free 0.227 |
| 5USA 5-Se-T2-DNA and native RNA hybrid in complex with RNase H catalytic domain D132N mutant Deposited 2017-02-13 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: trimeric |
Chain A
59–196（138 aa）
Fragment：residues 59-196
|
Mutation：D132N | MG MAGNESIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；0.1M MES pH 6.5, 12%(w/v) PEG 20000
|
Resolution 1.80 Å R-free 0.216 |
| 5USE 5-Se-T4-DNA and native RNA hybrid in complex with RNase H catalytic domain D132N mutant Deposited 2017-02-13 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: trimeric |
Chain A
59–196（138 aa）
Fragment：residues 59-196
|
Mutation：D132N | MG MAGNESIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；0.1M MES pH 6.5, 12%(w/v) PEG 20000
|
Resolution 1.73 Å R-free 0.234 |
| 5USG 5-Se-T2/4-DNA and native RNA hybrid in complex with RNase H catalytic domain D132N mutant Deposited 2017-02-13 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: trimeric |
Chain A
59–196（138 aa）
Fragment：residues 59-196
|
Mutation：D132N | MG MAGNESIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；0.1M MES pH 6.5, 12%(w/v) PEG 20000
|
Resolution 1.70 Å R-free 0.221 |
| 5VAJ BhRNase H - amide-RNA/DNA complex Deposited 2017-03-27 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Homooligomer；Protein × 2 PDB declaration: tetrameric |
Chain A
59–196（138 aa）
Chain B
59–196（138 aa）
|
Not recorded | GOL GLYCEROL × 9 MG MAGNESIUM ION × 4 ACT ACETATE ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；291 K；50 mM sodium cacodylate (pH 6.5) and 0.7 M sodium acetate.
|
Resolution 1.95 Å R-free 0.217 |
| 5W7N 2-Se-T2/4-DNA and native RNA hybrid in complex with RNase H catalytic domain D132N mutant Deposited 2017-06-20 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: trimeric |
Chain A
62–193（132 aa）
Fragment：residues 62-193
|
Mutation：D132N | MG MAGNESIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；0.1M MES pH 6.5, 12%(w/v) PEG 20000
|
Resolution 1.80 Å R-free 0.232 |
| 5W7O 2-Se-T4-DNA and native RNA hybrid in complex with RNase H catalytic domain D132N mutant Deposited 2017-06-20 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: trimeric |
Chain A
62–193（132 aa）
Fragment：residues 62-193
|
Mutation：D132N | MG MAGNESIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；0.1M MES pH 6.5, 12%(w/v) PEG 20000
|
Resolution 1.75 Å R-free 0.229 |
| 5WJR High resolution native hexamer DNA and RNA hybrid in complex with RNase H catalytic domain D132N mutant Deposited 2017-07-24 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: trimeric |
Chain A
59–196（138 aa）
Fragment：residues 59-196
|
Mutation：D132N | MG MAGNESIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；0.1M MES pH 6.5, 12%(w/v) PEG 20000
|
Resolution 1.70 Å R-free 0.234 |
| 6DMN Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Soaked in 2 mM Ca2+ and 200 mM K+ at 21 C Deposited 2018-06-05 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric |
Chain A
59–196（138 aa）
|
Not recorded | CA CALCIUM ION × 2 IOD IODIDE ION × 4 GOL GLYCEROL × 2 EDO 1,2-ETHANEDIOL × 4 K POTASSIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
|
Resolution 1.27 Å R-free 0.171 |
| 6DMV Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Soaked for 40 s in 2 mM Mg2+ and 200 mM K+ at 21 C Deposited 2018-06-05 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric |
Chain A
59–196（138 aa）
|
Not recorded | MG MAGNESIUM ION × 2 K POTASSIUM ION × 2 IOD IODIDE ION × 4 GOL GLYCEROL × 4 EDO 1,2-ETHANEDIOL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
|
Resolution 1.52 Å R-free 0.182 |
| 6DO8 Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 2 mM Mg2+ and 200 mM K+ for 80 s at 21 C Deposited 2018-06-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric |
Chain A
59–196（138 aa）
Fragment：Catalytic Domain
|
Not recorded | MG MAGNESIUM ION × 2 K POTASSIUM ION × 2 IOD IODIDE ION × 4 GOL GLYCEROL × 5 EDO 1,2-ETHANEDIOL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
|
Resolution 1.41 Å R-free 0.168 |
| 6DO9 Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 2 mM Mg2+ and 200 mM K+ for 120 s at 21 C Deposited 2018-06-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric |
Chain A
61–196（136 aa）
Fragment：Catalytic Domain
|
Not recorded | MG MAGNESIUM ION × 2 K POTASSIUM ION × 3 IOD IODIDE ION × 4 GOL GLYCEROL × 6 PGE TRIETHYLENE GLYCOL × 1 EDO 1,2-ETHANEDIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
|
Resolution 1.36 Å R-free 0.175 |
| 6DOA Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 2 mM Mg2+ and 200 mM K+ for 480 s at 21 C Deposited 2018-06-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric |
Chain A
61–196（136 aa）
Fragment：Catalytic Domain
|
Not recorded | MG MAGNESIUM ION × 2 K POTASSIUM ION × 3 IOD IODIDE ION × 4 EDO 1,2-ETHANEDIOL × 4 GOL GLYCEROL × 4 PGE TRIETHYLENE GLYCOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
|
Resolution 1.47 Å R-free 0.186 |
| 6DOB Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 2 mM Mg2+ and 200 mM K+ for 200 s at 21 C Deposited 2018-06-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric |
Chain A
59–196（138 aa）
Fragment：Catalytic Domain
|
Not recorded | MG MAGNESIUM ION × 2 K POTASSIUM ION × 3 IOD IODIDE ION × 4 EDO 1,2-ETHANEDIOL × 4 GOL GLYCEROL × 5 PGE TRIETHYLENE GLYCOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
|
Resolution 1.34 Å R-free 0.196 |
| 6DOC Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 2 mM Mg2+ and 200 mM K+ for 240 s at 21 C Deposited 2018-06-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric |
Chain A
59–196（138 aa）
Fragment：Catalytic Domain
|
Not recorded | MG MAGNESIUM ION × 2 K POTASSIUM ION × 3 IOD IODIDE ION × 4 EDO 1,2-ETHANEDIOL × 4 GOL GLYCEROL × 5 PGE TRIETHYLENE GLYCOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
|
Resolution 1.50 Å R-free 0.221 |
| 6DOD Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 2 mM Mg2+ and 200 mM K+ for 360 s at 21 C Deposited 2018-06-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric |
Chain A
59–196（138 aa）
Fragment：Catalytic Domain
|
Not recorded | MG MAGNESIUM ION × 2 K POTASSIUM ION × 3 IOD IODIDE ION × 4 EDO 1,2-ETHANEDIOL × 4 GOL GLYCEROL × 5 PGE TRIETHYLENE GLYCOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
|
Resolution 1.53 Å R-free 0.195 |
| 6DOE Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 2 mM Mg2+ and 200 mM K+ for 420 s at 21 C Deposited 2018-06-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric |
Chain A
61–196（136 aa）
Fragment：Catalytic Domain
|
Not recorded | MG MAGNESIUM ION × 2 K POTASSIUM ION × 3 IOD IODIDE ION × 4 EDO 1,2-ETHANEDIOL × 4 GOL GLYCEROL × 5 PGE TRIETHYLENE GLYCOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
|
Resolution 1.45 Å R-free 0.198 |
| 6DOF Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 2 mM Mg2+ and 200 mM K+ for 540 s at 21 C Deposited 2018-06-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric |
Chain A
59–196（138 aa）
Fragment：Catalytic Domain
|
Not recorded | MG MAGNESIUM ION × 2 K POTASSIUM ION × 3 IOD IODIDE ION × 4 EDO 1,2-ETHANEDIOL × 4 GOL GLYCEROL × 5 PGE TRIETHYLENE GLYCOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
|
Resolution 1.43 Å R-free 0.187 |
| 6DOG Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 2 mM Mg2+ and 200 mM K+ for 600 s at 21 C Deposited 2018-06-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric |
Chain A
59–196（138 aa）
Fragment：Catalytic Domain
|
Not recorded | MG MAGNESIUM ION × 2 K POTASSIUM ION × 3 IOD IODIDE ION × 4 EDO 1,2-ETHANEDIOL × 4 GOL GLYCEROL × 5 PGE TRIETHYLENE GLYCOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
|
Resolution 1.28 Å R-free 0.183 |
| 6DOH Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Soak in 0.5 mM EGTA and 200 mM K+ at 21 C Deposited 2018-06-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric |
Chain A
61–193（133 aa）
Fragment：Catalytic Domain
|
Not recorded | CA CALCIUM ION × 1 K POTASSIUM ION × 3 IOD IODIDE ION × 4 GOL GLYCEROL × 4 EDO 1,2-ETHANEDIOL × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
|
Resolution 1.36 Å R-free 0.200 |
| 6DOI Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid (1.54 Angstrom wavelength): Soak in 0.5 mM EGTA and 200 mM K+ at 21 C Deposited 2018-06-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric |
Chain A
61–193（133 aa）
Fragment：Catalytic Domain
|
Not recorded | K POTASSIUM ION × 3 CA CALCIUM ION × 1 IOD IODIDE ION × 4 GOL GLYCEROL × 4 EDO 1,2-ETHANEDIOL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
|
Resolution 1.95 Å R-free 0.195 |
| 6DOJ Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 2 mM Mg2+ and 5 mM K+ for 120 s at 21 C Deposited 2018-06-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric |
Chain A
61–195（135 aa）
Fragment：catalytic domain (UNP residues 61-196)
|
Not recorded | MG MAGNESIUM ION × 2 K POTASSIUM ION × 1 IOD IODIDE ION × 1 GOL GLYCEROL × 4 EDO 1,2-ETHANEDIOL × 5 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM potassium iodide, 5 mM calcium chloride
|
Resolution 1.40 Å R-free 0.185 |
| 6DOK Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 2 mM Mg2+ and 25 mM K+ for 120 s at 21 C (dataset 1) Deposited 2018-06-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric |
Chain A
61–195（135 aa）
Fragment：catalytic domain (UNP residues 61-196)
|
Not recorded | MG MAGNESIUM ION × 2 K POTASSIUM ION × 1 IOD IODIDE ION × 2 GOL GLYCEROL × 4 EDO 1,2-ETHANEDIOL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM potassium iodide, 5 mM calcium chloride
|
Resolution 1.38 Å R-free 0.170 |
| 6DOL Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 2 mM Mg2+ and 25 mM K+ for 120 s at 21 C (dataset 2) Deposited 2018-06-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric |
Chain A
61–195（135 aa）
Fragment：catalytic domain (UNP residues 61-196)
|
Not recorded | MG MAGNESIUM ION × 2 K POTASSIUM ION × 1 IOD IODIDE ION × 2 GOL GLYCEROL × 4 EDO 1,2-ETHANEDIOL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM potassium iodide, 5 mM calcium chloride
|
Resolution 1.43 Å R-free 0.185 |
| 6DOM Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 2 mM Mg2+ and 50 mM K+ for 120 s at 21 C Deposited 2018-06-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric |
Chain A
61–196（136 aa）
Fragment：catalytic domain (UNP residues 61-196)
|
Not recorded | MG MAGNESIUM ION × 2 K POTASSIUM ION × 3 IOD IODIDE ION × 3 GOL GLYCEROL × 5 EDO 1,2-ETHANEDIOL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM potassium iodide, 5 mM calcium chloride
|
Resolution 1.43 Å R-free 0.184 |
| 6DON Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 2 mM Mg2+ and 100 mM K+ for 120 s at 21 C (dataset 1) Deposited 2018-06-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric |
Chain A
61–196（136 aa）
Fragment：catalytic domain (UNP residues 61-196)
|
Not recorded | MG MAGNESIUM ION × 2 K POTASSIUM ION × 3 IOD IODIDE ION × 3 GOL GLYCEROL × 5 EDO 1,2-ETHANEDIOL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM potassium iodide, 5 mM calcium chloride
|
Resolution 1.42 Å R-free 0.195 |
| 6DOO Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 2 mM Mg2+ and 100 mM K+ for 120 s at 21 C (dataset 2) Deposited 2018-06-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric |
Chain A
61–196（136 aa）
Fragment：catalytic domain (UNP residues 61-196)
|
Not recorded | MG MAGNESIUM ION × 2 K POTASSIUM ION × 3 IOD IODIDE ION × 4 GOL GLYCEROL × 5 PGE TRIETHYLENE GLYCOL × 1 EDO 1,2-ETHANEDIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM potassium iodide, 5 mM calcium chloride
|
Resolution 1.44 Å R-free 0.170 |
| 6DOP Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 2 mM Mg2+ and 200 mM K+ for 120 s at 21 C (dataset 1) Deposited 2018-06-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric |
Chain A
59–196（138 aa）
Fragment：Catalytic Domain
|
Not recorded | MG MAGNESIUM ION × 2 K POTASSIUM ION × 2 IOD IODIDE ION × 4 GOL GLYCEROL × 6 PGE TRIETHYLENE GLYCOL × 1 EDO 1,2-ETHANEDIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
|
Resolution 1.25 Å R-free 0.178 |
| 6DOQ Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 2 mM Mg2+ and 200 mM K+ for 120 s at 21 C (dataset 2) Deposited 2018-06-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric |
Chain A
59–196（138 aa）
Fragment：Catalytic Domain
|
Not recorded | MG MAGNESIUM ION × 2 K POTASSIUM ION × 3 IOD IODIDE ION × 4 GOL GLYCEROL × 5 PGE TRIETHYLENE GLYCOL × 1 EDO 1,2-ETHANEDIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
|
Resolution 1.42 Å R-free 0.177 |
| 6DOR Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 2 mM Mg2+ and 300 mM K+ for 120 s at 21 C (dataset 1) Deposited 2018-06-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric |
Chain A
61–196（136 aa）
Fragment：catalytic domain (UNP residues 61-196)
|
Not recorded | MG MAGNESIUM ION × 2 K POTASSIUM ION × 3 IOD IODIDE ION × 4 GOL GLYCEROL × 5 PGE TRIETHYLENE GLYCOL × 1 EDO 1,2-ETHANEDIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM potassium iodide, 5 mM calcium chloride
|
Resolution 1.50 Å R-free 0.194 |
| 6DOS Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 2 mM Mg2+ and 300 mM K+ for 120 s at 21 C (dataset 2) Deposited 2018-06-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric |
Chain A
61–195（135 aa）
Fragment：catalytic domain (UNP residues 61-196)
|
Not recorded | MG MAGNESIUM ION × 2 K POTASSIUM ION × 2 IOD IODIDE ION × 4 GOL GLYCEROL × 6 PGE TRIETHYLENE GLYCOL × 1 EDO 1,2-ETHANEDIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM potassium iodide, 5 mM calcium chloride
|
Resolution 1.32 Å R-free 0.171 |
| 6DOT Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 5 mM Mg2+ and 200 mM Rb+ for 40 s at 21 C Deposited 2018-06-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric |
Chain A
61–196（136 aa）
Fragment：Catalytic Domain
|
Not recorded | MG MAGNESIUM ION × 2 RB RUBIDIUM ION × 3 CL CHLORIDE ION × 3 GOL GLYCEROL × 5 EDO 1,2-ETHANEDIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
|
Resolution 1.42 Å R-free 0.168 |
| 6DOU Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 5 mM Mg2+ and 200 mM Rb+ for 120 s at 21 C Deposited 2018-06-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric |
Chain A
61–196（136 aa）
Fragment：Catalytic Domain
|
Not recorded | MG MAGNESIUM ION × 2 RB RUBIDIUM ION × 5 CL CHLORIDE ION × 3 GOL GLYCEROL × 4 EDO 1,2-ETHANEDIOL × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
|
Resolution 1.49 Å R-free 0.188 |
| 6DOV Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 5 mM Mg2+ and 200 mM Rb+ for 80 s at 21 C Deposited 2018-06-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric |
Chain A
61–196（136 aa）
Fragment：Catalytic Domain
|
Not recorded | MG MAGNESIUM ION × 2 RB RUBIDIUM ION × 5 CL CHLORIDE ION × 3 GOL GLYCEROL × 4 EDO 1,2-ETHANEDIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
|
Resolution 1.52 Å R-free 0.190 |
| 6DOW Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 5 mM Mg2+ and 200 mM Rb+ for 160 s at 21 C Deposited 2018-06-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric |
Chain A
61–196（136 aa）
Fragment：Catalytic Domain
|
Not recorded | MG MAGNESIUM ION × 2 RB RUBIDIUM ION × 5 CL CHLORIDE ION × 3 GOL GLYCEROL × 3 EDO 1,2-ETHANEDIOL × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
|
Resolution 1.50 Å R-free 0.198 |
| 6DOX Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 5 mM Mg2+ and 200 mM Rb+ for 360 s at 21 C Deposited 2018-06-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric |
Chain A
61–196（136 aa）
Fragment：Catalytic Domain
|
Not recorded | MG MAGNESIUM ION × 2 RB RUBIDIUM ION × 5 CL CHLORIDE ION × 3 GOL GLYCEROL × 5 EDO 1,2-ETHANEDIOL × 6 PEG DI(HYDROXYETHYL)ETHER × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
|
Resolution 1.45 Å R-free 0.175 |
| 6DOY Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 2 mM Mg2+ and 200 mM Li+ for 120 s at 21 C Deposited 2018-06-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: trimeric |
Chain A
59–196（138 aa）
Fragment：Catalytic Domain
|
Not recorded | IOD IODIDE ION × 4 GOL GLYCEROL × 2 EDO 1,2-ETHANEDIOL × 8 LI LITHIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
|
Resolution 1.45 Å R-free 0.187 |
| 6DOZ Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 1 mM Mg2+ and 75 mM K+ for 40 s at 21 C Deposited 2018-06-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric |
Chain A
59–196（138 aa）
Fragment：Catalytic Domain residues 59-196
|
Not recorded | MG MAGNESIUM ION × 2 K POTASSIUM ION × 2 IOD IODIDE ION × 3 GOL GLYCEROL × 3 EDO 1,2-ETHANEDIOL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
|
Resolution 1.57 Å R-free 0.185 |
| 6DP0 Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 2.5 mM Mg2+ and 75 mM K+ for 40 s at 21 C Deposited 2018-06-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric |
Chain A
61–195（135 aa）
Fragment：Catalytic Domain residues 61-195
|
Not recorded | MG MAGNESIUM ION × 2 K POTASSIUM ION × 2 IOD IODIDE ION × 4 GOL GLYCEROL × 4 EDO 1,2-ETHANEDIOL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
|
Resolution 1.45 Å R-free 0.236 |
| 6DP1 Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 5 mM Mg2+ and 75 mM K+ for 40 s at 21 C Deposited 2018-06-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric |
Chain A
59–196（138 aa）
Fragment：Catalytic Domain residues 59-196
|
Not recorded | MG MAGNESIUM ION × 2 K POTASSIUM ION × 2 IOD IODIDE ION × 4 GOL GLYCEROL × 4 EDO 1,2-ETHANEDIOL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
|
Resolution 1.42 Å R-free 0.190 |
| 6DP2 Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 7.5 mM Mg2+ and 75 mM K+ for 40 s at 21 C Deposited 2018-06-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric |
Chain A
59–196（138 aa）
Fragment：Catalytic Domain residues 59-196
|
Not recorded | MG MAGNESIUM ION × 2 K POTASSIUM ION × 2 IOD IODIDE ION × 4 GOL GLYCEROL × 4 EDO 1,2-ETHANEDIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
|
Resolution 1.66 Å R-free 0.196 |
| 6DP3 Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 10 mM Mg2+ and 75 mM K+ for 40 s at 21 C Deposited 2018-06-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric |
Chain A
59–196（138 aa）
Fragment：Catalytic Domain residues 59-196
|
Not recorded | MG MAGNESIUM ION × 2 K POTASSIUM ION × 3 IOD IODIDE ION × 3 GOL GLYCEROL × 9 EDO 1,2-ETHANEDIOL × 6 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
|
Resolution 1.46 Å R-free 0.198 |
| 6DP4 Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 20 mM Mg2+ and 75 mM K+ for 40 s at 21 C Deposited 2018-06-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric |
Chain A
59–196（138 aa）
Fragment：Catalytic Domain residues 59-196
|
Not recorded | MG MAGNESIUM ION × 2 K POTASSIUM ION × 3 IOD IODIDE ION × 3 GOL GLYCEROL × 7 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
|
Resolution 1.37 Å R-free 0.176 |
| 6DP5 Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 40 mM Mg2+ and 75 mM K+ for 40 s at 21 C Deposited 2018-06-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric |
Chain A
59–196（138 aa）
Fragment：Catalytic Domain residues 59-196
|
Not recorded | MG MAGNESIUM ION × 2 K POTASSIUM ION × 3 IOD IODIDE ION × 4 GOL GLYCEROL × 2 EDO 1,2-ETHANEDIOL × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
|
Resolution 1.43 Å R-free 0.176 |
| 6DP6 Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 80 mM Mg2+ and 75 mM K+ for 40 s at 21 C Deposited 2018-06-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric |
Chain A
59–196（138 aa）
Fragment：Catalytic Domain residues 59-196
|
Not recorded | MG MAGNESIUM ION × 2 K POTASSIUM ION × 3 IOD IODIDE ION × 4 EDO 1,2-ETHANEDIOL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
|
Resolution 1.40 Å R-free 0.186 |
| 6DP7 Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 500 mM Mn2+ and 200 mM K+ for 40 s at 21 C Deposited 2018-06-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric |
Chain A
61–195（135 aa）
|
Not recorded | MN MANGANESE (II) ION × 4 K POTASSIUM ION × 1 IOD IODIDE ION × 3 EDO 1,2-ETHANEDIOL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
|
Resolution 1.38 Å R-free 0.192 |
| 6DP8 Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 5 mM Mg2+ and 200 mM Li+ for 240 s at 21 C Deposited 2018-06-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric |
Chain A
59–196（138 aa）
Fragment：Catalytic Domain
|
Not recorded | MG MAGNESIUM ION × 2 LI LITHIUM ION × 1 CL CHLORIDE ION × 3 GOL GLYCEROL × 3 EDO 1,2-ETHANEDIOL × 6 PEG DI(HYDROXYETHYL)ETHER × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
|
Resolution 1.32 Å R-free 0.170 |
| 6DP9 Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 2 mM Mn2+ and 200 mM K+ for 40 s at 21 C Deposited 2018-06-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric |
Chain A
61–196（136 aa）
|
Not recorded | MN MANGANESE (II) ION × 2 K POTASSIUM ION × 3 IOD IODIDE ION × 4 GOL GLYCEROL × 4 EDO 1,2-ETHANEDIOL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
|
Resolution 1.40 Å R-free 0.184 |
| 6DPA Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 4 mM Mn2+ and 200 mM K+ for 40 s at 21 C Deposited 2018-06-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric |
Chain A
61–196（136 aa）
|
Not recorded | MN MANGANESE (II) ION × 2 K POTASSIUM ION × 3 IOD IODIDE ION × 4 GOL GLYCEROL × 3 EDO 1,2-ETHANEDIOL × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
|
Resolution 1.49 Å R-free 0.186 |
| 6DPB Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 8 mM Mn2+ and 200 mM K+ for 40 s at 21 C Deposited 2018-06-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric |
Chain A
61–196（136 aa）
|
Not recorded | MN MANGANESE (II) ION × 4 K POTASSIUM ION × 3 IOD IODIDE ION × 4 GOL GLYCEROL × 4 EDO 1,2-ETHANEDIOL × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
|
Resolution 1.32 Å R-free 0.172 |
| 6DPC Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 12 mM Mn2+ and 200 mM K+ for 40 s at 21 C Deposited 2018-06-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric |
Chain A
61–196（136 aa）
|
Not recorded | MN MANGANESE (II) ION × 5 K POTASSIUM ION × 3 IOD IODIDE ION × 4 GOL GLYCEROL × 4 EDO 1,2-ETHANEDIOL × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
|
Resolution 1.34 Å R-free 0.180 |
| 6DPD Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 16 mM Mn2+ and 200 mM K+ for 40 s at 21 C Deposited 2018-06-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric |
Chain A
61–196（136 aa）
|
Not recorded | MN MANGANESE (II) ION × 6 K POTASSIUM ION × 1 IOD IODIDE ION × 5 GOL GLYCEROL × 3 EDO 1,2-ETHANEDIOL × 4 PGE TRIETHYLENE GLYCOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
|
Resolution 1.46 Å R-free 0.173 |
| 6DPE Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 20 mM Mn2+ and 200 mM K+ for 40 s at 21 C Deposited 2018-06-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric |
Chain A
61–196（136 aa）
|
Not recorded | MN MANGANESE (II) ION × 5 K POTASSIUM ION × 1 IOD IODIDE ION × 5 GOL GLYCEROL × 3 EDO 1,2-ETHANEDIOL × 4 PGE TRIETHYLENE GLYCOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
|
Resolution 1.56 Å R-free 0.217 |
| 6DPF Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 40 mM Mn2+ and 200 mM K+ for 40 s at 21 C Deposited 2018-06-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric |
Chain A
61–196（136 aa）
|
Not recorded | MN MANGANESE (II) ION × 5 IOD IODIDE ION × 5 GOL GLYCEROL × 3 EDO 1,2-ETHANEDIOL × 4 PGE TRIETHYLENE GLYCOL × 1 K POTASSIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
|
Resolution 1.56 Å R-free 0.207 |
| 6DPG Crystal Structure of Bacillus Halodurans Ribonuclease H1 E188A in Complex with an RNA/DNA Hybrid: Reaction in 4 mM Mn2+ and 200 mM K+ for 240 s at 21 C Deposited 2018-06-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric |
Chain A
59–196（138 aa）
Fragment：Catalytic Domain
|
Mutation：E188A | MN MANGANESE (II) ION × 4 IOD IODIDE ION × 3 GOL GLYCEROL × 2 EDO 1,2-ETHANEDIOL × 4 K POTASSIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
|
Resolution 1.38 Å R-free 0.175 |
| 6DPH Crystal Structure of Bacillus Halodurans Ribonuclease H1 E188A in Complex with an RNA/DNA Hybrid: Reaction in 2 mM Mn2+ and 200 mM K+ for 120 s at 21 C Deposited 2018-06-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric |
Chain A
59–196（138 aa）
Fragment：Catalytic Domain
|
Mutation：E188A | MG MAGNESIUM ION × 2 K POTASSIUM ION × 3 IOD IODIDE ION × 4 GOL GLYCEROL × 4 PGE TRIETHYLENE GLYCOL × 1 EDO 1,2-ETHANEDIOL × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
|
Resolution 1.34 Å R-free 0.192 |
| 6DPI Crystal Structure of Bacillus Halodurans Ribonuclease H1 K196A in Complex with an RNA/DNA Hybrid: Reaction in 10 mM Mg2+ and 200 mM Rb+ for 40 s at 21 C Deposited 2018-06-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric |
Chain A
59–196（138 aa）
Fragment：Catalytic Domain
|
Mutation：K196A | MG MAGNESIUM ION × 2 RB RUBIDIUM ION × 3 CL CHLORIDE ION × 3 GOL GLYCEROL × 4 EDO 1,2-ETHANEDIOL × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
|
Resolution 1.35 Å R-free 0.174 |
| 6DPJ Crystal Structure of Bacillus Halodurans Ribonuclease H1 K196A in Complex with an RNA/DNA Hybrid: Reaction in 4 mM Mn2+ and 200 mM K+ for 80 s at 21 C Deposited 2018-06-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric |
Chain A
59–196（138 aa）
Fragment：Catalytic Domain
|
Mutation：K196A | MN MANGANESE (II) ION × 2 K POTASSIUM ION × 2 IOD IODIDE ION × 4 EDO 1,2-ETHANEDIOL × 2 GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
|
Resolution 1.55 Å R-free 0.211 |
| 6DPK Crystal Structure of Bacillus Halodurans Ribonuclease H1 K196A in Complex with an RNA/DNA Hybrid: Reaction in 4 mM Mn2+ and 200 mM K+ for 240 s at 21 C Deposited 2018-06-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric |
Chain A
59–196（138 aa）
Fragment：Catalytic Domain
|
Mutation：K196A | MN MANGANESE (II) ION × 4 IOD IODIDE ION × 3 GOL GLYCEROL × 4 EDO 1,2-ETHANEDIOL × 5 K POTASSIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
|
Resolution 1.39 Å R-free 0.169 |
| 6DPL Crystal Structure of Bacillus Halodurans Ribonuclease H1 K196A in Complex with an RNA/DNA Hybrid: Reaction in 10 mM Mg2+ and 200 mM Rb+ for 720 s at 21 C Deposited 2018-06-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric |
Chain A
59–196（138 aa）
Fragment：Catalytic Domain
|
Mutation：K196A | MG MAGNESIUM ION × 2 RB RUBIDIUM ION × 6 CL CHLORIDE ION × 3 GOL GLYCEROL × 6 EDO 1,2-ETHANEDIOL × 5 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
|
Resolution 1.45 Å R-free 0.179 |
| 6DPM Crystal Structure of Bacillus Halodurans Ribonuclease H1 K196A in Complex with an RNA/DNA Hybrid: Reaction in 10 mM Mg2+ and 200 mM Rb+ for 1800 s at 21 C Deposited 2018-06-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric |
Chain A
59–196（138 aa）
Fragment：Catalytic Domain
|
Mutation：K196A | MG MAGNESIUM ION × 2 RB RUBIDIUM ION × 6 CL CHLORIDE ION × 3 GOL GLYCEROL × 4 EDO 1,2-ETHANEDIOL × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
|
Resolution 1.68 Å R-free 0.199 |
| 6DPN Crystal Structure of Bacillus Halodurans Ribonuclease H1 E188A in Complex with an RNA/DNA Hybrid: Reaction in 2 mM Mg2+ and 200 mM K+ for 200 s at 21 C Deposited 2018-06-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric |
Chain A
59–196（138 aa）
Fragment：Catalytic Domain
|
Mutation：E188A | MG MAGNESIUM ION × 2 K POTASSIUM ION × 2 IOD IODIDE ION × 4 GOL GLYCEROL × 4 EDO 1,2-ETHANEDIOL × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
|
Resolution 1.49 Å R-free 0.179 |
| 6DPO Crystal Structure of Bacillus Halodurans Ribonuclease H1 E188A in Complex with an RNA/DNA Hybrid: Reaction in 2 mM Mg2+ and 200 mM K+ for 360 s at 21 C Deposited 2018-06-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric |
Chain A
59–196（138 aa）
Fragment：Catalytic Domain
|
Mutation：E188A | MG MAGNESIUM ION × 3 IOD IODIDE ION × 4 GOL GLYCEROL × 5 EDO 1,2-ETHANEDIOL × 3 K POTASSIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
|
Resolution 1.45 Å R-free 0.186 |
| 6DPP Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 5 mM Mg2+ and 200 mM Rb+ for 240 s at 21 C Deposited 2018-06-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric |
Chain A
61–196（136 aa）
Fragment：Catalytic Domain
|
Not recorded | MG MAGNESIUM ION × 2 RB RUBIDIUM ION × 5 CL CHLORIDE ION × 3 GOL GLYCEROL × 3 EDO 1,2-ETHANEDIOL × 5 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
|
Resolution 1.45 Å R-free 0.171 |
| 8CTY 12-mer DNA structure of ExBIM bound to RNase-H Deposited 2022-05-16 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: tetrameric |
Chain A
59–196（138 aa）
Chain F
59–196（138 aa）
|
Mutation：D132N Mutation：D132N | EDO 1,2-ETHANEDIOL × 2 GOL GLYCEROL × 1 NA SODIUM ION × 1 ACT ACETATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；291 K；0.1 M HEPES sodium pH = 7.5, 10% v/v 2-propanol and 20% w/v PEG 4000
|
Resolution 2.30 Å R-free 0.261 |
| 8CTY 12-mer DNA structure of ExBIM bound to RNase-H Deposited 2022-05-16 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein–DNA Homooligomer；Protein × 3 PDB declaration: pentameric |
Chain B
59–196（138 aa）
Chain C
59–196（138 aa）
Chain G
59–196（138 aa）
|
Mutation：D132N Mutation：D132N Mutation：D132N | EDO 1,2-ETHANEDIOL × 5 CL CHLORIDE ION × 1 GOL GLYCEROL × 3 PEG DI(HYDROXYETHYL)ETHER × 1 ACT ACETATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；291 K；0.1 M HEPES sodium pH = 7.5, 10% v/v 2-propanol and 20% w/v PEG 4000
|
Resolution 2.30 Å R-free 0.261 |
| 8CTY 12-mer DNA structure of ExBIM bound to RNase-H Deposited 2022-05-16 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein–DNA Homooligomer；Protein × 3 PDB declaration: pentameric |
Chain D
59–196（138 aa）
Chain E
59–196（138 aa）
Chain H
59–196（138 aa）
|
Mutation：D132N Mutation：D132N Mutation：D132N | EDO 1,2-ETHANEDIOL × 2 GOL GLYCEROL × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；291 K；0.1 M HEPES sodium pH = 7.5, 10% v/v 2-propanol and 20% w/v PEG 4000
|
Resolution 2.30 Å R-free 0.261 |
| 8CTZ 12-mer DNA structure of ExBIM & O6Me-G bound to RNase-H Deposited 2022-05-16 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–DNA Homooligomer；Protein × 3 PDB declaration: pentameric |
Chain A
59–196（138 aa）
Chain B
59–196（138 aa）
Chain C
59–196（138 aa）
|
Mutation：D132N Mutation：D132N Mutation：D132N | GOL GLYCEROL × 4 EDO 1,2-ETHANEDIOL × 3 ACT ACETATE ION × 2 NA SODIUM ION × 1 K POTASSIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；291 K；0.1 M HEPES, 10% v/v 2-propanol and 20% w/v PEG 4000
|
Resolution 2.32 Å R-free 0.265 |
| 8CU0 12-mer DNA structure of ExBIM bound to RNaseH -modified DDD Deposited 2022-05-16 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–DNA Homooligomer；Protein × 3 PDB declaration: pentameric |
Chain A
59–196（138 aa）
Chain B
59–196（138 aa）
Chain C
59–196（138 aa）
|
Mutation：D132N Mutation：D132N Mutation：D132N | EDO 1,2-ETHANEDIOL × 5 GOL GLYCEROL × 3 NA SODIUM ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；291 K；0.2 M sodium acetate trihydrate, 0.1 M sodium cacodylate trihydrate, 30% PEG 8000 (W/V)
|
Resolution 1.74 Å R-free 0.246 |
| 8SV3 7-Deazapurines and 5-Halogenpyrimidine DNA duplex Deposited 2023-05-15 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–DNA Monomer；Protein × 1 PDB declaration: trimeric |
Chain A
59–196（138 aa）
|
Mutation：D132N | MG MAGNESIUM ION × 1 GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6；291 K；2 M Ammonium Sulphate
|
Resolution 1.51 Å R-free 0.210 |
| 8SV3 7-Deazapurines and 5-Halogenpyrimidine DNA duplex Deposited 2023-05-15 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
59–196（138 aa）
|
Mutation：D132N | NA SODIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6；291 K；2 M Ammonium Sulphate
|
Resolution 1.51 Å R-free 0.210 |
| 8SV3 7-Deazapurines and 5-Halogenpyrimidine DNA duplex Deposited 2023-05-15 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
59–196（138 aa）
|
Mutation：D132N | NA SODIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6；291 K；2 M Ammonium Sulphate
|
Resolution 1.51 Å R-free 0.210 |
| 8SV4 7-Deazapurines and 5-Halogenpyrimidine DNA duplex Deposited 2023-05-15 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–DNA Monomer；Protein × 1 PDB declaration: trimeric |
Chain A
59–196（138 aa）
|
Mutation：D132N | FWN 2-[2-(2-ethoxyethoxy)ethoxy]ethanol × 1 ACT ACETATE ION × 2 SO4 SULFATE ION × 2 EDO 1,2-ETHANEDIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 4.6；291 K；0.2 M Ammonium Sulfate, 0.1 M Sodium acetate trihydrate and 25% (w/v) PEG 4000
|
Resolution 2.30 Å R-free 0.254 |
| 9YJL Joint X-ray/neutron structure of wild-type Bacillus halodurans RNase H1 in the apo-form Deposited 2025-10-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
59–196（138 aa）
|
Not recorded | SO4 SULFATE ION × 1 |
Experimental method not declared
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5；291 K；0.1 M NaOAc pH 5.0, 0.2 M (NH4)2SO4, and 20% PEG 3350
|
Resolution not provided |
| 9YJM Joint X-ray/neutron structure of D132N Bacillus halodurans RNase H1 in the apo-form Deposited 2025-10-03 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
59–196（138 aa）
|
Mutation：D132N | SO4 SULFATE ION × 1 |
Experimental method not declared
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5；291 K；0.1 M NaOAc pH 5.0, 0.2 M (NH4)2SO4, and 20% PEG 3350
|
Resolution not provided |
| 9YK1 Room-temperature X-ray structure of D132N Bacillus halodurans RNase H1 in complex with RNA/DNA duplex Deposited 2025-10-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: trimeric |
Chain A
59–196（138 aa）
|
Not recorded | MG MAGNESIUM ION × 1 PO3 PHOSPHITE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；0.1 M HEPES pH 7.5, 0.2 M NaCl, and 12-16% PEG 10,000
|
Resolution 1.80 Å R-free 0.216 |
| 9YK3 Room-temperature X-ray structure of D132N Bacillus halodurans RNase H1 in complex with complementary RNA/DNA duplex Deposited 2025-10-06 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: trimeric |
Chain A
59–196（138 aa）
|
Mutation：D132N | MG MAGNESIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；0.1 M HEPES pH 7.5, 0.2 M NaCl, and 12-16% PEG 10,000
|
Resolution 1.90 Å R-free 0.231 |
| 9YK5 100K X-ray structure of mixed metal D132N Bacillus halodurans RNase H1 complex with RNA/DNA duplex Deposited 2025-10-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: trimeric |
Chain A
59–196（138 aa）
|
Not recorded | MN MANGANESE (II) ION × 1 MG MAGNESIUM ION × 1 PO3 PHOSPHITE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；0.1 M HEPES pH 7.5, 0.2 M NaCl, and 12-16% PEG 10,000
|
Resolution 2.00 Å R-free 0.278 |
95 other PDB entries and 107 assemblies. Open the comparison page and filter oligomeric states
View Construct and Data Evidence
| UniProt name | RNH1_BACHD |
| Isoform | — |
| PDB entities | 1 |
| Chains and sequence ranges | Author chain A； PDBConstruct 1–134； UniProt 61–194 Author chain C； PDBConstruct 1–134； UniProt 61–194 |
