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8ctz

12-mer DNA structure of ExBIM & O6Me-G bound to RNase-H

Method: X-RAY DIFFRACTION Dmax: 90.1 Å Quality: EXCELLENT
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Ribonuclease H

Alkalihalobacillus halodurans

UniProt Q9KEI9

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–DNA Homooligomer Protein × 3 DNA 2 PDB declaration: pentameric（5） Consistent with all polymer counts Chain A； UniProt 59–196 Chain B； UniProt 59–196 Chain C； UniProt 59–196 Mutation：D132N ;DNA (5'-D(*CP*GP*CP*(6OG)P*AP*AP*TP*TP*(OWR)P*GP*CP*G)-3') ; × 2 GOL GLYCEROL × 4 EDO 1,2-ETHANEDIOL × 3 ACT ACETATE ION × 2 NA SODIUM ION × 1 K POTASSIUM ION × 2 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；pH 7.5；291 K；0.1 M HEPES, 10% v/v 2-propanol and 20% w/v PEG 4000 Resolution 2.32 Å R-free 0.265

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8CTZ Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
1ZBF Crystal structure of B. halodurans RNase H catalytic domain mutant D132N Deposited 2005-04-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 59–196（138 aa） Fragment：catalytic domain (residues 59-196)
Mutation：D132N SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4.6；277 K；PEG 2000 monomethyl ether, ammonium sulfate, sodium acetate, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 277K
Resolution 1.50 Å R-free 0.214
1ZBI Bacillus halodurans RNase H catalytic domain mutant D132N in complex with 12-mer RNA/DNA hybrid Deposited 2005-04-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Homooligomer；Protein × 2 PDB declaration: tetrameric
Chain A 59–196（138 aa） Fragment：catalytic domain (residues 59-196)
Chain B 59–196（138 aa） Fragment：catalytic domain (residues 59-196)
Mutation：D132N Mutation：D132N MG MAGNESIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；294 K；MPD, NaCl, HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K
Resolution 1.85 Å R-free 0.248
2G8F B. halodurans RNase H catalytic domain E188A mutant in complex with Mg2+ and RNA/DNA hybrid (non-P nick at the active site) Deposited 2006-03-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: trimeric
Chain A 59–196（138 aa） Fragment：Bh-RNase HC
Mutation：E188A MG MAGNESIUM ION × 2 CL CHLORIDE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 5.6；298 K；30% MPD, 0.1M citrate pH 5.6, 0.25M ammonium acetate, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 1.65 Å
2G8H B. halodurans RNase H catalytic domain D192N mutant in complex with Mg2+ and RNA/DNA hybrid (non-P nick at the active site) Deposited 2006-03-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: trimeric
Chain A 59–196（138 aa） Fragment：Bh-RNase HC
Mutation：D192N MG MAGNESIUM ION × 2 VO4 VANADATE ION × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；278 K；32% MPD, 0.1M Tris pH 7.0, 0.2 M NaCl, VAPOR DIFFUSION, SITTING DROP, temperature 278K
Resolution 1.85 Å R-free 0.254
2G8I B. halodurans RNase H catalytic domain D192N mutant in complex with Mn2+ and RNA/DNA hybrid (non-P nick at the active site) Deposited 2006-03-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: trimeric
Chain A 59–196（138 aa） Fragment：Bh-RNase HC
Mutation：D192N MN MANGANESE (II) ION × 4 CL CHLORIDE ION × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；278 K；35% MPD, 0.1M Tris pH 7.0, 0.2 M NaCl, VAPOR DIFFUSION, SITTING DROP, temperature 278K
Resolution 1.65 Å R-free 0.202
2G8K B. halodurans RNase H catalytic domain D192N mutant in complex with Ca2+ and RNA/DNA hybrid (non-P nick at the active site) Deposited 2006-03-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: trimeric
Chain A 59–196（138 aa） Fragment：Bh-RNase HC
Mutation：D192N CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 5.6；278 K；27% MPD, 0.25M ammonium acetate, Na Citrate pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 278K
Resolution 1.65 Å R-free 0.210
2G8U B. halodurans RNase H catalytic domain D132N mutant in complex with Mg2+ and RNA/DNA hybrid (non-P nick at the active site) Deposited 2006-03-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: trimeric
Chain A 59–196（138 aa） Fragment：Bh-RNase HC
Mutation：D132N MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；278 K；25% MPD, 0.2M NaCl, 0.1M HEPES pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 278K
Resolution 2.70 Å R-free 0.288
2G8V B. halodurans RNase H catalytic domain E188A mutant in complex with Mg2+ and RNA/DNA hybrid (reaction product) Deposited 2006-03-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: trimeric
Chain A 59–196（138 aa） Fragment：Bh-RNase HC
Mutation：E188A MG MAGNESIUM ION × 2 EOH ETHANOL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；278 K；20% EtOH, 0.1 M Tris pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 278K
Resolution 1.85 Å R-free 0.240
2G8W B. halodurans RNase H catalytic domain E188A mutant in complex with Ca2+ and RNA/DNA hybrid Deposited 2006-03-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: trimeric
Chain A 59–196（138 aa） Fragment：Bh-RNase HC
Mutation：E188A CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；278 K；12% isopropanol, 0.1M MES 6.0, 0.3M CaAc2, VAPOR DIFFUSION, SITTING DROP, temperature 278K
Resolution 2.05 Å R-free 0.242
2R7Y Selenium Derivatized RNA/DNA Hybrid in complex with RNase H CATALYTIC DOMAIN MUTANT D132N Deposited 2007-09-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: trimeric
Chain A 62–193（132 aa）
Mutation：D132N PO4 PHOSPHATE ION × 1 MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；0.1 M MES, 12% PEG 20000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 1.80 Å R-free 0.231
3D0P Insights into RNA/DNA hybrid recognition and processing by RNase H from the crystal structure of a non-specific enzyme-dsDNA complex Deposited 2008-05-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: tetrameric
Chain A 61–194（134 aa）
Mutation：D132N No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4.6；291 K；0.1 M sodium acetate, 8 % (w/v) PEG 4000, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 291K
Resolution 1.80 Å R-free 0.241
3D0P Insights into RNA/DNA hybrid recognition and processing by RNase H from the crystal structure of a non-specific enzyme-dsDNA complex Deposited 2008-05-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–DNA Homooligomer；Protein × 2 PDB declaration: tetrameric
Chain C 61–194（134 aa）
Mutation：D132N NA SODIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4.6；291 K；0.1 M sodium acetate, 8 % (w/v) PEG 4000, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 291K
Resolution 1.80 Å R-free 0.241
3EY1 A Conformational Transition in the Structure of a 2'-Thiomethyl-Modified DNA Visualized at High Resolution Deposited 2008-10-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: tetrameric
Chain A 59–196（138 aa） Fragment：RNase H domain, UNP residues 59-196
Mutation：D132N GOL GLYCEROL × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4.6；291 K；0.1 M Sodium Acetate (pH 4.6), 0.2 M ammonium sulphate, 25% PEG 4000., VAPOR DIFFUSION, SITTING DROP, temperature 291K
Resolution 1.60 Å R-free 0.261
3I8D The Pairing Geometry of the Hydrophobic Thymine Analog 2,4-Difluorotoluene in Duplex DNA as Analyzed by X-ray Crystallography Deposited 2009-07-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: tetrameric
Chain A 62–193（132 aa） Fragment：RNase-H
Mutation：D132N GOL GLYCEROL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4.6；291 K；0.1 M NaOAc 3H2O (pH 4.6) and 8% (w/v) PEG 4000 , VAPOR DIFFUSION, SITTING DROP, temperature 291K
Resolution 1.61 Å R-free 0.238
3I8D The Pairing Geometry of the Hydrophobic Thymine Analog 2,4-Difluorotoluene in Duplex DNA as Analyzed by X-ray Crystallography Deposited 2009-07-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–DNA Homooligomer；Protein × 2 PDB declaration: tetrameric
Chain C 62–193（132 aa） Fragment：RNase-H
Mutation：D132N GOL GLYCEROL × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4.6；291 K；0.1 M NaOAc 3H2O (pH 4.6) and 8% (w/v) PEG 4000 , VAPOR DIFFUSION, SITTING DROP, temperature 291K
Resolution 1.61 Å R-free 0.238
3TWH Selenium Derivatized RNA/DNA Hybrid in complex with RNase H Catalytic Domain D132N Mutant Deposited 2011-09-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: trimeric
Chain A 59–196（138 aa） Fragment：catalytic domain
Mutation：D132N MG MAGNESIUM ION × 2 PO4 PHOSPHATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；0.1 M MES, 12% PEG 20000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 1.79 Å R-free 0.208
3ULD High resolution structure of DNA/RNA hybrid in complex with RNase H catalytic domain D132N mutant Deposited 2011-11-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: trimeric
Chain A 59–196（138 aa） Fragment：catalytic domain
Mutation：D132N MG MAGNESIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；298 K；0.04 M Magnesium chloride, 0.05 M Sodium cacodylate, 5% MPD, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 1.60 Å R-free 0.235
4HTU Structure of 5-chlorouracil modified A:U base pair Deposited 2012-11-01 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Monomer；Protein × 1 PDB declaration: trimeric
Chain A 61–194（134 aa）
Not recorded MG MAGNESIUM ION × 1 GOL GLYCEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.5；277 K；0.2 M magnesium acetate, 0.1 M sodium cacodylate (pH 6.5) and 20% (w/v) PEG 8000, VAPOR DIFFUSION, SITTING DROP, temperature 277K
Resolution 1.49 Å R-free 0.218
4HTU Structure of 5-chlorouracil modified A:U base pair Deposited 2012-11-01 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–DNA Monomer；Protein × 1 PDB declaration: trimeric
Chain B 61–194（134 aa）
Not recorded MG MAGNESIUM ION × 1 GOL GLYCEROL × 2 EDO 1,2-ETHANEDIOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.5；277 K；0.2 M magnesium acetate, 0.1 M sodium cacodylate (pH 6.5) and 20% (w/v) PEG 8000, VAPOR DIFFUSION, SITTING DROP, temperature 277K
Resolution 1.49 Å R-free 0.218
4HUE Structure of 5-chlorouracil modified G:U base pair Deposited 2012-11-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Monomer；Protein × 1 PDB declaration: trimeric
Chain A 61–194（134 aa）
Mutation：D132N GOL GLYCEROL × 3 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.5；277 K；0.2 M magnesium acetate, 0.1 M sodium cacodylate and 20% (w/v) PEG 8000, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K
Resolution 1.56 Å R-free 0.220
4HUE Structure of 5-chlorouracil modified G:U base pair Deposited 2012-11-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–DNA Monomer；Protein × 1 PDB declaration: trimeric
Chain B 61–194（134 aa）
Mutation：D132N GOL GLYCEROL × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.5；277 K；0.2 M magnesium acetate, 0.1 M sodium cacodylate and 20% (w/v) PEG 8000, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K
Resolution 1.56 Å R-free 0.220
4HUF Structure of 5-chlorouracil modified A:U base pair Deposited 2012-11-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Monomer；Protein × 1 PDB declaration: trimeric
Chain A 61–194（134 aa）
Mutation：D132N GOL GLYCEROL × 2 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；0.2 M magnesium acetate, 0.1 M sodium cacodylate (pH 6.5) and 20% (w/v) PEG 8000 , VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.69 Å R-free 0.228
4HUF Structure of 5-chlorouracil modified A:U base pair Deposited 2012-11-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–DNA Monomer；Protein × 1 PDB declaration: trimeric
Chain B 61–194（134 aa）
Mutation：D132N MG MAGNESIUM ION × 1 EDO 1,2-ETHANEDIOL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；0.2 M magnesium acetate, 0.1 M sodium cacodylate (pH 6.5) and 20% (w/v) PEG 8000 , VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.69 Å R-free 0.228
4HUG Structure of 5-chlorouracil modified A:U base pairs Deposited 2012-11-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Monomer；Protein × 1 PDB declaration: trimeric
Chain A 61–194（134 aa）
Mutation：D132N MG MAGNESIUM ION × 1 GOL GLYCEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.5；277 K；0.2 M magnesium acetate, 0.1 M sodium cacodylate and 20% (w/v) PEG 8000 , pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K
Resolution 1.64 Å R-free 0.222
4HUG Structure of 5-chlorouracil modified A:U base pairs Deposited 2012-11-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–DNA Monomer；Protein × 1 PDB declaration: trimeric
Chain B 61–194（134 aa）
Mutation：D132N MG MAGNESIUM ION × 1 GOL GLYCEROL × 1 EDO 1,2-ETHANEDIOL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.5；277 K；0.2 M magnesium acetate, 0.1 M sodium cacodylate and 20% (w/v) PEG 8000 , pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K
Resolution 1.64 Å R-free 0.222
4OPJ Bh-RNaseH:tcdA-DNA complex Deposited 2014-02-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Monomer；Protein × 1 PDB declaration: trimeric
Chain A 59–196（138 aa） Fragment：UNP residues 59-196
Mutation：D132N GOL GLYCEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4.6；291 K；0.1 M sodium acetate, 10% w/v PEG4000, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 291K
Resolution 1.54 Å R-free 0.220
4OPJ Bh-RNaseH:tcdA-DNA complex Deposited 2014-02-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–DNA Monomer；Protein × 1 PDB declaration: trimeric
Chain C 59–196（138 aa） Fragment：UNP residues 59-196
Mutation：D132N GOL GLYCEROL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4.6；291 K；0.1 M sodium acetate, 10% w/v PEG4000, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 291K
Resolution 1.54 Å R-free 0.220
4OPK Bh-RNaseH:2'-SMe-DNA complex Deposited 2014-02-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Monomer；Protein × 1 PDB declaration: trimeric
Chain A 59–196（138 aa） Fragment：UNP residues 59-196
Mutation：D132N GOL GLYCEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4.6；291 K；0.1 M sodium acetate, 8% w/v PEG4000, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 291K
Resolution 1.54 Å R-free 0.258
4OPK Bh-RNaseH:2'-SMe-DNA complex Deposited 2014-02-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–DNA Monomer；Protein × 1 PDB declaration: trimeric
Chain C 59–196（138 aa） Fragment：UNP residues 59-196
Mutation：D132N GOL GLYCEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4.6；291 K；0.1 M sodium acetate, 8% w/v PEG4000, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 291K
Resolution 1.54 Å R-free 0.258
5SWM BACILLUS HALODURANS RNASE H MUTANT D132N IN COMPLEX WITH 12-MER FRNA/DNA HYBRID Deposited 2016-08-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: trimeric
Chain A 59–196（138 aa）
Mutation：D132N NA SODIUM ION × 1 CL CHLORIDE ION × 1 PEG DI(HYDROXYETHYL)ETHER × 1 GOL GLYCEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；291 K；0.4 M ammonium phosphate monobasic
Resolution 1.50 Å R-free 0.185
5SWM BACILLUS HALODURANS RNASE H MUTANT D132N IN COMPLEX WITH 12-MER FRNA/DNA HYBRID Deposited 2016-08-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Other combination Monomer；Protein × 1 PDB declaration: trimeric
Chain B 59–196（138 aa）
Mutation：D132N GOL GLYCEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；291 K；0.4 M ammonium phosphate monobasic
Resolution 1.50 Å R-free 0.185
5US2 2-Se-T2-DNA and native RNA hybrid in complex with RNase H catalytic domain D132N mutant Deposited 2017-02-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: trimeric
Chain A 59–196（138 aa） Fragment：residues 59-196
Mutation：D132N MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；0.1M MES pH 6.5, 12%(w/v) PEG 20000
Resolution 1.90 Å R-free 0.227
5USA 5-Se-T2-DNA and native RNA hybrid in complex with RNase H catalytic domain D132N mutant Deposited 2017-02-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: trimeric
Chain A 59–196（138 aa） Fragment：residues 59-196
Mutation：D132N MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；0.1M MES pH 6.5, 12%(w/v) PEG 20000
Resolution 1.80 Å R-free 0.216
5USE 5-Se-T4-DNA and native RNA hybrid in complex with RNase H catalytic domain D132N mutant Deposited 2017-02-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: trimeric
Chain A 59–196（138 aa） Fragment：residues 59-196
Mutation：D132N MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；0.1M MES pH 6.5, 12%(w/v) PEG 20000
Resolution 1.73 Å R-free 0.234
5USG 5-Se-T2/4-DNA and native RNA hybrid in complex with RNase H catalytic domain D132N mutant Deposited 2017-02-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: trimeric
Chain A 59–196（138 aa） Fragment：residues 59-196
Mutation：D132N MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；0.1M MES pH 6.5, 12%(w/v) PEG 20000
Resolution 1.70 Å R-free 0.221
5VAJ BhRNase H - amide-RNA/DNA complex Deposited 2017-03-27 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Homooligomer；Protein × 2 PDB declaration: tetrameric
Chain A 59–196（138 aa）
Chain B 59–196（138 aa）
Not recorded GOL GLYCEROL × 9 MG MAGNESIUM ION × 4 ACT ACETATE ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.5；291 K；50 mM sodium cacodylate (pH 6.5) and 0.7 M sodium acetate.
Resolution 1.95 Å R-free 0.217
5W7N 2-Se-T2/4-DNA and native RNA hybrid in complex with RNase H catalytic domain D132N mutant Deposited 2017-06-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: trimeric
Chain A 62–193（132 aa） Fragment：residues 62-193
Mutation：D132N MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；0.1M MES pH 6.5, 12%(w/v) PEG 20000
Resolution 1.80 Å R-free 0.232
5W7O 2-Se-T4-DNA and native RNA hybrid in complex with RNase H catalytic domain D132N mutant Deposited 2017-06-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: trimeric
Chain A 62–193（132 aa） Fragment：residues 62-193
Mutation：D132N MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；0.1M MES pH 6.5, 12%(w/v) PEG 20000
Resolution 1.75 Å R-free 0.229
5WJR High resolution native hexamer DNA and RNA hybrid in complex with RNase H catalytic domain D132N mutant Deposited 2017-07-24 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: trimeric
Chain A 59–196（138 aa） Fragment：residues 59-196
Mutation：D132N MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；0.1M MES pH 6.5, 12%(w/v) PEG 20000
Resolution 1.70 Å R-free 0.234
6DMN Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Soaked in 2 mM Ca2+ and 200 mM K+ at 21 C Deposited 2018-06-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric
Chain A 59–196（138 aa）
Not recorded CA CALCIUM ION × 2 IOD IODIDE ION × 4 GOL GLYCEROL × 2 EDO 1,2-ETHANEDIOL × 4 K POTASSIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
Resolution 1.27 Å R-free 0.171
6DMV Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Soaked for 40 s in 2 mM Mg2+ and 200 mM K+ at 21 C Deposited 2018-06-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric
Chain A 59–196（138 aa）
Not recorded MG MAGNESIUM ION × 2 K POTASSIUM ION × 2 IOD IODIDE ION × 4 GOL GLYCEROL × 4 EDO 1,2-ETHANEDIOL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
Resolution 1.52 Å R-free 0.182
6DO8 Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 2 mM Mg2+ and 200 mM K+ for 80 s at 21 C Deposited 2018-06-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric
Chain A 59–196（138 aa） Fragment：Catalytic Domain
Not recorded MG MAGNESIUM ION × 2 K POTASSIUM ION × 2 IOD IODIDE ION × 4 GOL GLYCEROL × 5 EDO 1,2-ETHANEDIOL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
Resolution 1.41 Å R-free 0.168
6DO9 Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 2 mM Mg2+ and 200 mM K+ for 120 s at 21 C Deposited 2018-06-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric
Chain A 61–196（136 aa） Fragment：Catalytic Domain
Not recorded MG MAGNESIUM ION × 2 K POTASSIUM ION × 3 IOD IODIDE ION × 4 GOL GLYCEROL × 6 PGE TRIETHYLENE GLYCOL × 1 EDO 1,2-ETHANEDIOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
Resolution 1.36 Å R-free 0.175
6DOA Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 2 mM Mg2+ and 200 mM K+ for 480 s at 21 C Deposited 2018-06-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric
Chain A 61–196（136 aa） Fragment：Catalytic Domain
Not recorded MG MAGNESIUM ION × 2 K POTASSIUM ION × 3 IOD IODIDE ION × 4 EDO 1,2-ETHANEDIOL × 4 GOL GLYCEROL × 4 PGE TRIETHYLENE GLYCOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
Resolution 1.47 Å R-free 0.186
6DOB Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 2 mM Mg2+ and 200 mM K+ for 200 s at 21 C Deposited 2018-06-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric
Chain A 59–196（138 aa） Fragment：Catalytic Domain
Not recorded MG MAGNESIUM ION × 2 K POTASSIUM ION × 3 IOD IODIDE ION × 4 EDO 1,2-ETHANEDIOL × 4 GOL GLYCEROL × 5 PGE TRIETHYLENE GLYCOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
Resolution 1.34 Å R-free 0.196
6DOC Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 2 mM Mg2+ and 200 mM K+ for 240 s at 21 C Deposited 2018-06-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric
Chain A 59–196（138 aa） Fragment：Catalytic Domain
Not recorded MG MAGNESIUM ION × 2 K POTASSIUM ION × 3 IOD IODIDE ION × 4 EDO 1,2-ETHANEDIOL × 4 GOL GLYCEROL × 5 PGE TRIETHYLENE GLYCOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
Resolution 1.50 Å R-free 0.221
6DOD Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 2 mM Mg2+ and 200 mM K+ for 360 s at 21 C Deposited 2018-06-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric
Chain A 59–196（138 aa） Fragment：Catalytic Domain
Not recorded MG MAGNESIUM ION × 2 K POTASSIUM ION × 3 IOD IODIDE ION × 4 EDO 1,2-ETHANEDIOL × 4 GOL GLYCEROL × 5 PGE TRIETHYLENE GLYCOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
Resolution 1.53 Å R-free 0.195
6DOE Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 2 mM Mg2+ and 200 mM K+ for 420 s at 21 C Deposited 2018-06-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric
Chain A 61–196（136 aa） Fragment：Catalytic Domain
Not recorded MG MAGNESIUM ION × 2 K POTASSIUM ION × 3 IOD IODIDE ION × 4 EDO 1,2-ETHANEDIOL × 4 GOL GLYCEROL × 5 PGE TRIETHYLENE GLYCOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
Resolution 1.45 Å R-free 0.198
6DOF Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 2 mM Mg2+ and 200 mM K+ for 540 s at 21 C Deposited 2018-06-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric
Chain A 59–196（138 aa） Fragment：Catalytic Domain
Not recorded MG MAGNESIUM ION × 2 K POTASSIUM ION × 3 IOD IODIDE ION × 4 EDO 1,2-ETHANEDIOL × 4 GOL GLYCEROL × 5 PGE TRIETHYLENE GLYCOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
Resolution 1.43 Å R-free 0.187
6DOG Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 2 mM Mg2+ and 200 mM K+ for 600 s at 21 C Deposited 2018-06-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric
Chain A 59–196（138 aa） Fragment：Catalytic Domain
Not recorded MG MAGNESIUM ION × 2 K POTASSIUM ION × 3 IOD IODIDE ION × 4 EDO 1,2-ETHANEDIOL × 4 GOL GLYCEROL × 5 PGE TRIETHYLENE GLYCOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
Resolution 1.28 Å R-free 0.183
6DOH Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Soak in 0.5 mM EGTA and 200 mM K+ at 21 C Deposited 2018-06-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric
Chain A 61–193（133 aa） Fragment：Catalytic Domain
Not recorded CA CALCIUM ION × 1 K POTASSIUM ION × 3 IOD IODIDE ION × 4 GOL GLYCEROL × 4 EDO 1,2-ETHANEDIOL × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
Resolution 1.36 Å R-free 0.200
6DOI Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid (1.54 Angstrom wavelength): Soak in 0.5 mM EGTA and 200 mM K+ at 21 C Deposited 2018-06-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric
Chain A 61–193（133 aa） Fragment：Catalytic Domain
Not recorded K POTASSIUM ION × 3 CA CALCIUM ION × 1 IOD IODIDE ION × 4 GOL GLYCEROL × 4 EDO 1,2-ETHANEDIOL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
Resolution 1.95 Å R-free 0.195
6DOJ Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 2 mM Mg2+ and 5 mM K+ for 120 s at 21 C Deposited 2018-06-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric
Chain A 61–195（135 aa） Fragment：catalytic domain (UNP residues 61-196)
Not recorded MG MAGNESIUM ION × 2 K POTASSIUM ION × 1 IOD IODIDE ION × 1 GOL GLYCEROL × 4 EDO 1,2-ETHANEDIOL × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM potassium iodide, 5 mM calcium chloride
Resolution 1.40 Å R-free 0.185
6DOK Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 2 mM Mg2+ and 25 mM K+ for 120 s at 21 C (dataset 1) Deposited 2018-06-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric
Chain A 61–195（135 aa） Fragment：catalytic domain (UNP residues 61-196)
Not recorded MG MAGNESIUM ION × 2 K POTASSIUM ION × 1 IOD IODIDE ION × 2 GOL GLYCEROL × 4 EDO 1,2-ETHANEDIOL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM potassium iodide, 5 mM calcium chloride
Resolution 1.38 Å R-free 0.170
6DOL Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 2 mM Mg2+ and 25 mM K+ for 120 s at 21 C (dataset 2) Deposited 2018-06-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric
Chain A 61–195（135 aa） Fragment：catalytic domain (UNP residues 61-196)
Not recorded MG MAGNESIUM ION × 2 K POTASSIUM ION × 1 IOD IODIDE ION × 2 GOL GLYCEROL × 4 EDO 1,2-ETHANEDIOL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM potassium iodide, 5 mM calcium chloride
Resolution 1.43 Å R-free 0.185
6DOM Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 2 mM Mg2+ and 50 mM K+ for 120 s at 21 C Deposited 2018-06-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric
Chain A 61–196（136 aa） Fragment：catalytic domain (UNP residues 61-196)
Not recorded MG MAGNESIUM ION × 2 K POTASSIUM ION × 3 IOD IODIDE ION × 3 GOL GLYCEROL × 5 EDO 1,2-ETHANEDIOL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM potassium iodide, 5 mM calcium chloride
Resolution 1.43 Å R-free 0.184
6DON Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 2 mM Mg2+ and 100 mM K+ for 120 s at 21 C (dataset 1) Deposited 2018-06-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric
Chain A 61–196（136 aa） Fragment：catalytic domain (UNP residues 61-196)
Not recorded MG MAGNESIUM ION × 2 K POTASSIUM ION × 3 IOD IODIDE ION × 3 GOL GLYCEROL × 5 EDO 1,2-ETHANEDIOL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM potassium iodide, 5 mM calcium chloride
Resolution 1.42 Å R-free 0.195
6DOO Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 2 mM Mg2+ and 100 mM K+ for 120 s at 21 C (dataset 2) Deposited 2018-06-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric
Chain A 61–196（136 aa） Fragment：catalytic domain (UNP residues 61-196)
Not recorded MG MAGNESIUM ION × 2 K POTASSIUM ION × 3 IOD IODIDE ION × 4 GOL GLYCEROL × 5 PGE TRIETHYLENE GLYCOL × 1 EDO 1,2-ETHANEDIOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM potassium iodide, 5 mM calcium chloride
Resolution 1.44 Å R-free 0.170
6DOP Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 2 mM Mg2+ and 200 mM K+ for 120 s at 21 C (dataset 1) Deposited 2018-06-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric
Chain A 59–196（138 aa） Fragment：Catalytic Domain
Not recorded MG MAGNESIUM ION × 2 K POTASSIUM ION × 2 IOD IODIDE ION × 4 GOL GLYCEROL × 6 PGE TRIETHYLENE GLYCOL × 1 EDO 1,2-ETHANEDIOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
Resolution 1.25 Å R-free 0.178
6DOQ Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 2 mM Mg2+ and 200 mM K+ for 120 s at 21 C (dataset 2) Deposited 2018-06-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric
Chain A 59–196（138 aa） Fragment：Catalytic Domain
Not recorded MG MAGNESIUM ION × 2 K POTASSIUM ION × 3 IOD IODIDE ION × 4 GOL GLYCEROL × 5 PGE TRIETHYLENE GLYCOL × 1 EDO 1,2-ETHANEDIOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
Resolution 1.42 Å R-free 0.177
6DOR Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 2 mM Mg2+ and 300 mM K+ for 120 s at 21 C (dataset 1) Deposited 2018-06-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric
Chain A 61–196（136 aa） Fragment：catalytic domain (UNP residues 61-196)
Not recorded MG MAGNESIUM ION × 2 K POTASSIUM ION × 3 IOD IODIDE ION × 4 GOL GLYCEROL × 5 PGE TRIETHYLENE GLYCOL × 1 EDO 1,2-ETHANEDIOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM potassium iodide, 5 mM calcium chloride
Resolution 1.50 Å R-free 0.194
6DOS Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 2 mM Mg2+ and 300 mM K+ for 120 s at 21 C (dataset 2) Deposited 2018-06-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric
Chain A 61–195（135 aa） Fragment：catalytic domain (UNP residues 61-196)
Not recorded MG MAGNESIUM ION × 2 K POTASSIUM ION × 2 IOD IODIDE ION × 4 GOL GLYCEROL × 6 PGE TRIETHYLENE GLYCOL × 1 EDO 1,2-ETHANEDIOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM potassium iodide, 5 mM calcium chloride
Resolution 1.32 Å R-free 0.171
6DOT Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 5 mM Mg2+ and 200 mM Rb+ for 40 s at 21 C Deposited 2018-06-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric
Chain A 61–196（136 aa） Fragment：Catalytic Domain
Not recorded MG MAGNESIUM ION × 2 RB RUBIDIUM ION × 3 CL CHLORIDE ION × 3 GOL GLYCEROL × 5 EDO 1,2-ETHANEDIOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
Resolution 1.42 Å R-free 0.168
6DOU Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 5 mM Mg2+ and 200 mM Rb+ for 120 s at 21 C Deposited 2018-06-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric
Chain A 61–196（136 aa） Fragment：Catalytic Domain
Not recorded MG MAGNESIUM ION × 2 RB RUBIDIUM ION × 5 CL CHLORIDE ION × 3 GOL GLYCEROL × 4 EDO 1,2-ETHANEDIOL × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
Resolution 1.49 Å R-free 0.188
6DOV Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 5 mM Mg2+ and 200 mM Rb+ for 80 s at 21 C Deposited 2018-06-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric
Chain A 61–196（136 aa） Fragment：Catalytic Domain
Not recorded MG MAGNESIUM ION × 2 RB RUBIDIUM ION × 5 CL CHLORIDE ION × 3 GOL GLYCEROL × 4 EDO 1,2-ETHANEDIOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
Resolution 1.52 Å R-free 0.190
6DOW Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 5 mM Mg2+ and 200 mM Rb+ for 160 s at 21 C Deposited 2018-06-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric
Chain A 61–196（136 aa） Fragment：Catalytic Domain
Not recorded MG MAGNESIUM ION × 2 RB RUBIDIUM ION × 5 CL CHLORIDE ION × 3 GOL GLYCEROL × 3 EDO 1,2-ETHANEDIOL × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
Resolution 1.50 Å R-free 0.198
6DOX Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 5 mM Mg2+ and 200 mM Rb+ for 360 s at 21 C Deposited 2018-06-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric
Chain A 61–196（136 aa） Fragment：Catalytic Domain
Not recorded MG MAGNESIUM ION × 2 RB RUBIDIUM ION × 5 CL CHLORIDE ION × 3 GOL GLYCEROL × 5 EDO 1,2-ETHANEDIOL × 6 PEG DI(HYDROXYETHYL)ETHER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
Resolution 1.45 Å R-free 0.175
6DOY Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 2 mM Mg2+ and 200 mM Li+ for 120 s at 21 C Deposited 2018-06-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: trimeric
Chain A 59–196（138 aa） Fragment：Catalytic Domain
Not recorded IOD IODIDE ION × 4 GOL GLYCEROL × 2 EDO 1,2-ETHANEDIOL × 8 LI LITHIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
Resolution 1.45 Å R-free 0.187
6DOZ Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 1 mM Mg2+ and 75 mM K+ for 40 s at 21 C Deposited 2018-06-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric
Chain A 59–196（138 aa） Fragment：Catalytic Domain residues 59-196
Not recorded MG MAGNESIUM ION × 2 K POTASSIUM ION × 2 IOD IODIDE ION × 3 GOL GLYCEROL × 3 EDO 1,2-ETHANEDIOL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
Resolution 1.57 Å R-free 0.185
6DP0 Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 2.5 mM Mg2+ and 75 mM K+ for 40 s at 21 C Deposited 2018-06-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric
Chain A 61–195（135 aa） Fragment：Catalytic Domain residues 61-195
Not recorded MG MAGNESIUM ION × 2 K POTASSIUM ION × 2 IOD IODIDE ION × 4 GOL GLYCEROL × 4 EDO 1,2-ETHANEDIOL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
Resolution 1.45 Å R-free 0.236
6DP1 Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 5 mM Mg2+ and 75 mM K+ for 40 s at 21 C Deposited 2018-06-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric
Chain A 59–196（138 aa） Fragment：Catalytic Domain residues 59-196
Not recorded MG MAGNESIUM ION × 2 K POTASSIUM ION × 2 IOD IODIDE ION × 4 GOL GLYCEROL × 4 EDO 1,2-ETHANEDIOL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
Resolution 1.42 Å R-free 0.190
6DP2 Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 7.5 mM Mg2+ and 75 mM K+ for 40 s at 21 C Deposited 2018-06-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric
Chain A 59–196（138 aa） Fragment：Catalytic Domain residues 59-196
Not recorded MG MAGNESIUM ION × 2 K POTASSIUM ION × 2 IOD IODIDE ION × 4 GOL GLYCEROL × 4 EDO 1,2-ETHANEDIOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
Resolution 1.66 Å R-free 0.196
6DP3 Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 10 mM Mg2+ and 75 mM K+ for 40 s at 21 C Deposited 2018-06-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric
Chain A 59–196（138 aa） Fragment：Catalytic Domain residues 59-196
Not recorded MG MAGNESIUM ION × 2 K POTASSIUM ION × 3 IOD IODIDE ION × 3 GOL GLYCEROL × 9 EDO 1,2-ETHANEDIOL × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
Resolution 1.46 Å R-free 0.198
6DP4 Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 20 mM Mg2+ and 75 mM K+ for 40 s at 21 C Deposited 2018-06-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric
Chain A 59–196（138 aa） Fragment：Catalytic Domain residues 59-196
Not recorded MG MAGNESIUM ION × 2 K POTASSIUM ION × 3 IOD IODIDE ION × 3 GOL GLYCEROL × 7 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
Resolution 1.37 Å R-free 0.176
6DP5 Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 40 mM Mg2+ and 75 mM K+ for 40 s at 21 C Deposited 2018-06-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric
Chain A 59–196（138 aa） Fragment：Catalytic Domain residues 59-196
Not recorded MG MAGNESIUM ION × 2 K POTASSIUM ION × 3 IOD IODIDE ION × 4 GOL GLYCEROL × 2 EDO 1,2-ETHANEDIOL × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
Resolution 1.43 Å R-free 0.176
6DP6 Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 80 mM Mg2+ and 75 mM K+ for 40 s at 21 C Deposited 2018-06-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric
Chain A 59–196（138 aa） Fragment：Catalytic Domain residues 59-196
Not recorded MG MAGNESIUM ION × 2 K POTASSIUM ION × 3 IOD IODIDE ION × 4 EDO 1,2-ETHANEDIOL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
Resolution 1.40 Å R-free 0.186
6DP7 Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 500 mM Mn2+ and 200 mM K+ for 40 s at 21 C Deposited 2018-06-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric
Chain A 61–195（135 aa）
Not recorded MN MANGANESE (II) ION × 4 K POTASSIUM ION × 1 IOD IODIDE ION × 3 EDO 1,2-ETHANEDIOL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
Resolution 1.38 Å R-free 0.192
6DP8 Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 5 mM Mg2+ and 200 mM Li+ for 240 s at 21 C Deposited 2018-06-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric
Chain A 59–196（138 aa） Fragment：Catalytic Domain
Not recorded MG MAGNESIUM ION × 2 LI LITHIUM ION × 1 CL CHLORIDE ION × 3 GOL GLYCEROL × 3 EDO 1,2-ETHANEDIOL × 6 PEG DI(HYDROXYETHYL)ETHER × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
Resolution 1.32 Å R-free 0.170
6DP9 Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 2 mM Mn2+ and 200 mM K+ for 40 s at 21 C Deposited 2018-06-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric
Chain A 61–196（136 aa）
Not recorded MN MANGANESE (II) ION × 2 K POTASSIUM ION × 3 IOD IODIDE ION × 4 GOL GLYCEROL × 4 EDO 1,2-ETHANEDIOL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
Resolution 1.40 Å R-free 0.184
6DPA Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 4 mM Mn2+ and 200 mM K+ for 40 s at 21 C Deposited 2018-06-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric
Chain A 61–196（136 aa）
Not recorded MN MANGANESE (II) ION × 2 K POTASSIUM ION × 3 IOD IODIDE ION × 4 GOL GLYCEROL × 3 EDO 1,2-ETHANEDIOL × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
Resolution 1.49 Å R-free 0.186
6DPB Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 8 mM Mn2+ and 200 mM K+ for 40 s at 21 C Deposited 2018-06-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric
Chain A 61–196（136 aa）
Not recorded MN MANGANESE (II) ION × 4 K POTASSIUM ION × 3 IOD IODIDE ION × 4 GOL GLYCEROL × 4 EDO 1,2-ETHANEDIOL × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
Resolution 1.32 Å R-free 0.172
6DPC Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 12 mM Mn2+ and 200 mM K+ for 40 s at 21 C Deposited 2018-06-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric
Chain A 61–196（136 aa）
Not recorded MN MANGANESE (II) ION × 5 K POTASSIUM ION × 3 IOD IODIDE ION × 4 GOL GLYCEROL × 4 EDO 1,2-ETHANEDIOL × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
Resolution 1.34 Å R-free 0.180
6DPD Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 16 mM Mn2+ and 200 mM K+ for 40 s at 21 C Deposited 2018-06-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric
Chain A 61–196（136 aa）
Not recorded MN MANGANESE (II) ION × 6 K POTASSIUM ION × 1 IOD IODIDE ION × 5 GOL GLYCEROL × 3 EDO 1,2-ETHANEDIOL × 4 PGE TRIETHYLENE GLYCOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
Resolution 1.46 Å R-free 0.173
6DPE Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 20 mM Mn2+ and 200 mM K+ for 40 s at 21 C Deposited 2018-06-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric
Chain A 61–196（136 aa）
Not recorded MN MANGANESE (II) ION × 5 K POTASSIUM ION × 1 IOD IODIDE ION × 5 GOL GLYCEROL × 3 EDO 1,2-ETHANEDIOL × 4 PGE TRIETHYLENE GLYCOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
Resolution 1.56 Å R-free 0.217
6DPF Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 40 mM Mn2+ and 200 mM K+ for 40 s at 21 C Deposited 2018-06-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric
Chain A 61–196（136 aa）
Not recorded MN MANGANESE (II) ION × 5 IOD IODIDE ION × 5 GOL GLYCEROL × 3 EDO 1,2-ETHANEDIOL × 4 PGE TRIETHYLENE GLYCOL × 1 K POTASSIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
Resolution 1.56 Å R-free 0.207
6DPG Crystal Structure of Bacillus Halodurans Ribonuclease H1 E188A in Complex with an RNA/DNA Hybrid: Reaction in 4 mM Mn2+ and 200 mM K+ for 240 s at 21 C Deposited 2018-06-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric
Chain A 59–196（138 aa） Fragment：Catalytic Domain
Mutation：E188A MN MANGANESE (II) ION × 4 IOD IODIDE ION × 3 GOL GLYCEROL × 2 EDO 1,2-ETHANEDIOL × 4 K POTASSIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
Resolution 1.38 Å R-free 0.175
6DPH Crystal Structure of Bacillus Halodurans Ribonuclease H1 E188A in Complex with an RNA/DNA Hybrid: Reaction in 2 mM Mn2+ and 200 mM K+ for 120 s at 21 C Deposited 2018-06-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric
Chain A 59–196（138 aa） Fragment：Catalytic Domain
Mutation：E188A MG MAGNESIUM ION × 2 K POTASSIUM ION × 3 IOD IODIDE ION × 4 GOL GLYCEROL × 4 PGE TRIETHYLENE GLYCOL × 1 EDO 1,2-ETHANEDIOL × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
Resolution 1.34 Å R-free 0.192
6DPI Crystal Structure of Bacillus Halodurans Ribonuclease H1 K196A in Complex with an RNA/DNA Hybrid: Reaction in 10 mM Mg2+ and 200 mM Rb+ for 40 s at 21 C Deposited 2018-06-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric
Chain A 59–196（138 aa） Fragment：Catalytic Domain
Mutation：K196A MG MAGNESIUM ION × 2 RB RUBIDIUM ION × 3 CL CHLORIDE ION × 3 GOL GLYCEROL × 4 EDO 1,2-ETHANEDIOL × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
Resolution 1.35 Å R-free 0.174
6DPJ Crystal Structure of Bacillus Halodurans Ribonuclease H1 K196A in Complex with an RNA/DNA Hybrid: Reaction in 4 mM Mn2+ and 200 mM K+ for 80 s at 21 C Deposited 2018-06-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric
Chain A 59–196（138 aa） Fragment：Catalytic Domain
Mutation：K196A MN MANGANESE (II) ION × 2 K POTASSIUM ION × 2 IOD IODIDE ION × 4 EDO 1,2-ETHANEDIOL × 2 GOL GLYCEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
Resolution 1.55 Å R-free 0.211
6DPK Crystal Structure of Bacillus Halodurans Ribonuclease H1 K196A in Complex with an RNA/DNA Hybrid: Reaction in 4 mM Mn2+ and 200 mM K+ for 240 s at 21 C Deposited 2018-06-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric
Chain A 59–196（138 aa） Fragment：Catalytic Domain
Mutation：K196A MN MANGANESE (II) ION × 4 IOD IODIDE ION × 3 GOL GLYCEROL × 4 EDO 1,2-ETHANEDIOL × 5 K POTASSIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
Resolution 1.39 Å R-free 0.169
6DPL Crystal Structure of Bacillus Halodurans Ribonuclease H1 K196A in Complex with an RNA/DNA Hybrid: Reaction in 10 mM Mg2+ and 200 mM Rb+ for 720 s at 21 C Deposited 2018-06-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric
Chain A 59–196（138 aa） Fragment：Catalytic Domain
Mutation：K196A MG MAGNESIUM ION × 2 RB RUBIDIUM ION × 6 CL CHLORIDE ION × 3 GOL GLYCEROL × 6 EDO 1,2-ETHANEDIOL × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
Resolution 1.45 Å R-free 0.179
6DPM Crystal Structure of Bacillus Halodurans Ribonuclease H1 K196A in Complex with an RNA/DNA Hybrid: Reaction in 10 mM Mg2+ and 200 mM Rb+ for 1800 s at 21 C Deposited 2018-06-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric
Chain A 59–196（138 aa） Fragment：Catalytic Domain
Mutation：K196A MG MAGNESIUM ION × 2 RB RUBIDIUM ION × 6 CL CHLORIDE ION × 3 GOL GLYCEROL × 4 EDO 1,2-ETHANEDIOL × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
Resolution 1.68 Å R-free 0.199
6DPN Crystal Structure of Bacillus Halodurans Ribonuclease H1 E188A in Complex with an RNA/DNA Hybrid: Reaction in 2 mM Mg2+ and 200 mM K+ for 200 s at 21 C Deposited 2018-06-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric
Chain A 59–196（138 aa） Fragment：Catalytic Domain
Mutation：E188A MG MAGNESIUM ION × 2 K POTASSIUM ION × 2 IOD IODIDE ION × 4 GOL GLYCEROL × 4 EDO 1,2-ETHANEDIOL × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
Resolution 1.49 Å R-free 0.179
6DPO Crystal Structure of Bacillus Halodurans Ribonuclease H1 E188A in Complex with an RNA/DNA Hybrid: Reaction in 2 mM Mg2+ and 200 mM K+ for 360 s at 21 C Deposited 2018-06-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric
Chain A 59–196（138 aa） Fragment：Catalytic Domain
Mutation：E188A MG MAGNESIUM ION × 3 IOD IODIDE ION × 4 GOL GLYCEROL × 5 EDO 1,2-ETHANEDIOL × 3 K POTASSIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
Resolution 1.45 Å R-free 0.186
6DPP Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 5 mM Mg2+ and 200 mM Rb+ for 240 s at 21 C Deposited 2018-06-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: tetrameric
Chain A 61–196（136 aa） Fragment：Catalytic Domain
Not recorded MG MAGNESIUM ION × 2 RB RUBIDIUM ION × 5 CL CHLORIDE ION × 3 GOL GLYCEROL × 3 EDO 1,2-ETHANEDIOL × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2
Resolution 1.45 Å R-free 0.171
8CTY 12-mer DNA structure of ExBIM bound to RNase-H Deposited 2022-05-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: tetrameric
Chain A 59–196（138 aa）
Chain F 59–196（138 aa）
Mutation：D132N Mutation：D132N EDO 1,2-ETHANEDIOL × 2 GOL GLYCEROL × 1 NA SODIUM ION × 1 ACT ACETATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；291 K；0.1 M HEPES sodium pH = 7.5, 10% v/v 2-propanol and 20% w/v PEG 4000
Resolution 2.30 Å R-free 0.261
8CTY 12-mer DNA structure of ExBIM bound to RNase-H Deposited 2022-05-16 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–DNA Homooligomer；Protein × 3 PDB declaration: pentameric
Chain B 59–196（138 aa）
Chain C 59–196（138 aa）
Chain G 59–196（138 aa）
Mutation：D132N Mutation：D132N Mutation：D132N EDO 1,2-ETHANEDIOL × 5 CL CHLORIDE ION × 1 GOL GLYCEROL × 3 PEG DI(HYDROXYETHYL)ETHER × 1 ACT ACETATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；291 K；0.1 M HEPES sodium pH = 7.5, 10% v/v 2-propanol and 20% w/v PEG 4000
Resolution 2.30 Å R-free 0.261
8CTY 12-mer DNA structure of ExBIM bound to RNase-H Deposited 2022-05-16 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein–DNA Homooligomer；Protein × 3 PDB declaration: pentameric
Chain D 59–196（138 aa）
Chain E 59–196（138 aa）
Chain H 59–196（138 aa）
Mutation：D132N Mutation：D132N Mutation：D132N EDO 1,2-ETHANEDIOL × 2 GOL GLYCEROL × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；291 K；0.1 M HEPES sodium pH = 7.5, 10% v/v 2-propanol and 20% w/v PEG 4000
Resolution 2.30 Å R-free 0.261
8CU0 12-mer DNA structure of ExBIM bound to RNaseH -modified DDD Deposited 2022-05-16 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Homooligomer；Protein × 3 PDB declaration: pentameric
Chain A 59–196（138 aa）
Chain B 59–196（138 aa）
Chain C 59–196（138 aa）
Mutation：D132N Mutation：D132N Mutation：D132N EDO 1,2-ETHANEDIOL × 5 GOL GLYCEROL × 3 NA SODIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；291 K；0.2 M sodium acetate trihydrate, 0.1 M sodium cacodylate trihydrate, 30% PEG 8000 (W/V)
Resolution 1.74 Å R-free 0.246
8SV3 7-Deazapurines and 5-Halogenpyrimidine DNA duplex Deposited 2023-05-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Monomer；Protein × 1 PDB declaration: trimeric
Chain A 59–196（138 aa）
Mutation：D132N MG MAGNESIUM ION × 1 GOL GLYCEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；291 K；2 M Ammonium Sulphate
Resolution 1.51 Å R-free 0.210
8SV3 7-Deazapurines and 5-Halogenpyrimidine DNA duplex Deposited 2023-05-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 59–196（138 aa）
Mutation：D132N NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；291 K；2 M Ammonium Sulphate
Resolution 1.51 Å R-free 0.210
8SV3 7-Deazapurines and 5-Halogenpyrimidine DNA duplex Deposited 2023-05-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain C 59–196（138 aa）
Mutation：D132N NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；291 K；2 M Ammonium Sulphate
Resolution 1.51 Å R-free 0.210
8SV4 7-Deazapurines and 5-Halogenpyrimidine DNA duplex Deposited 2023-05-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–DNA Monomer；Protein × 1 PDB declaration: trimeric
Chain A 59–196（138 aa）
Mutation：D132N FWN 2-[2-(2-ethoxyethoxy)ethoxy]ethanol × 1 ACT ACETATE ION × 2 SO4 SULFATE ION × 2 EDO 1,2-ETHANEDIOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4.6；291 K；0.2 M Ammonium Sulfate, 0.1 M Sodium acetate trihydrate and 25% (w/v) PEG 4000
Resolution 2.30 Å R-free 0.254
9YJL Joint X-ray/neutron structure of wild-type Bacillus halodurans RNase H1 in the apo-form Deposited 2025-10-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 59–196（138 aa）
Not recorded SO4 SULFATE ION × 1 Experimental method not declared
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 5；291 K；0.1 M NaOAc pH 5.0, 0.2 M (NH4)2SO4, and 20% PEG 3350
Resolution not provided
9YJM Joint X-ray/neutron structure of D132N Bacillus halodurans RNase H1 in the apo-form Deposited 2025-10-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 59–196（138 aa）
Mutation：D132N SO4 SULFATE ION × 1 Experimental method not declared
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 5；291 K；0.1 M NaOAc pH 5.0, 0.2 M (NH4)2SO4, and 20% PEG 3350
Resolution not provided
9YK1 Room-temperature X-ray structure of D132N Bacillus halodurans RNase H1 in complex with RNA/DNA duplex Deposited 2025-10-06 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: trimeric
Chain A 59–196（138 aa）
Not recorded MG MAGNESIUM ION × 1 PO3 PHOSPHITE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；0.1 M HEPES pH 7.5, 0.2 M NaCl, and 12-16% PEG 10,000
Resolution 1.80 Å R-free 0.216
9YK3 Room-temperature X-ray structure of D132N Bacillus halodurans RNase H1 in complex with complementary RNA/DNA duplex Deposited 2025-10-06 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: trimeric
Chain A 59–196（138 aa）
Mutation：D132N MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；0.1 M HEPES pH 7.5, 0.2 M NaCl, and 12-16% PEG 10,000
Resolution 1.90 Å R-free 0.231
9YK5 100K X-ray structure of mixed metal D132N Bacillus halodurans RNase H1 complex with RNA/DNA duplex Deposited 2025-10-06 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: trimeric
Chain A 59–196（138 aa）
Not recorded MN MANGANESE (II) ION × 1 MG MAGNESIUM ION × 1 PO3 PHOSPHITE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；0.1 M HEPES pH 7.5, 0.2 M NaCl, and 12-16% PEG 10,000
Resolution 2.00 Å R-free 0.278

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View Construct and Data Evidence
UniProt name RNH1_BACHD
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 5–142； UniProt 59–196 Author chain B； PDBConstruct 5–142； UniProt 59–196 Author chain C； PDBConstruct 5–142； UniProt 59–196

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 8ctz

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 8ctz
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2. Structure Basics 2. Structure Basics

Entry ID entry_id8ctz
Deposition date deposition_date2022-05-16
Structure title title12-mer DNA structure of ExBIM & O6Me-G bound to RNase-H
Keywords keywords;alkylation, base stacking, DNA damage, H-bonding, O6-methyl-2'-deoxyguanosine, ExBIM, DNA ;; DNA
Experimental Method methodX-RAY DIFFRACTION
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier28.18 Å
Radius of gyration Rg (electron density) rg_electron26.66 Å
Forward intensity I(0) i052354100.00
Molecular weight molecular_weight53180.0 kDa
Excluded volume excluded_volume65199 ų
Envelope volume envelope_volume84994 ų
Hydration-shell volume shell_volume27785 ų
Envelope diameter envelope_diameter92.3 Å
Shell Rg shell_rg32.45 Å
Envelope Rg envelope_rg26.66 Å
Shape Rg shape_rg26.60 Å
Total Rg total_rg27.44 Å
Total atoms total_atoms3726
Residues n_residues412
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax90.1 Å
Rg (real space) rg_real28.15 Å
Rg uncertainty (real space) rg_real_error0.57 Å
I(0) (real space) i0_real5.2350e+07
I(0) uncertainty (real space) i0_real_error8.1870e+05
Rg (reciprocal space) rg_reciprocal28.16 Å
I(0) (reciprocal space) i0_reciprocal52350000.0000
Solution quality estimate total_estimate0.9071
Solution quality rating solution_quality EXCELLENT a EXCELLENT solution
P(r) peaks n_peaks1
Primary peak position r_peak_primary88.5 Å
Skewness Skewness skewness0.226
Kurtosis Kurtosis kurtosis-0.585
Angular range angular_range— – 0.2800 Å−1
Current regularization parameter α current_alpha0.0000
Highest regularization parameter α highest_alpha5703000.0000
Real-space data points n_real_points57
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.955; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.976; Smooth: 0.946
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (8)

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8. Citations (1)

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9. Files and Curves (10)