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8b3g

C(N)RL4CSA-UVSSA-E2-ubiquitin complex.

Method: ELECTRON MICROSCOPY
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1. Protein Identity and Related Structures Protein Identity & Related Structures

ubiquitin-conjugating enzyme E2 D2 isoform X3

Homo sapiens

UniProt A0A6P5C4T4

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 8 NEDD8 × 1 （Q15843） E3 ubiquitin-protein ligase RBX1 × 1 （P62877） Ubiquitin × 1 （P0CG48） DNA excision repair protein ERCC-8 × 1 （Q13216） UV-stimulated scaffold protein A × 1 （Q2YD98） DNA damage-binding protein 1 × 1 （Q16531） Cullin-4A × 1 （Q13619） ZINC ION × 3 Consistent with protein count

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
8b3g

1 assemblies

View Construct and Data Evidence
UniProt name A0A6P5C4T4_BOSIN
Isoform —
PDB entities 1
Chains and sequence ranges Author chain D； PDBConstruct 1–147； UniProt 1–147

NEDD8

Homo sapiens

UniProt Q15843

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 8 ubiquitin-conjugating enzyme E2 D2 isoform X3 × 1 （A0A6P5C4T4） E3 ubiquitin-protein ligase RBX1 × 1 （P62877） Ubiquitin × 1 （P0CG48） DNA excision repair protein ERCC-8 × 1 （Q13216） UV-stimulated scaffold protein A × 1 （Q2YD98） DNA damage-binding protein 1 × 1 （Q16531） Cullin-4A × 1 （Q13619） ZINC ION × 3 Consistent with protein count

Other States of the Same Protein in the Database

Monomer
1ndd 1ndd 1ndd 1ndd 2ko3

5 assemblies

Homodimer

None

Homotetramer
1ndd

1 assemblies

Other homooligomers

None

Heteromeric or nucleic-acid complex
1r4m 1r4m 1r4m 1r4m 1r4n 1r4n 1r4n 1r4n 1xt9 2bkr 2n7k 2nvu

66 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name NEDD8_HUMAN
Isoform —
PDB entities 2
Chains and sequence ranges Author chain N； PDBConstruct 1–76； UniProt 1–76

E3 ubiquitin-protein ligase RBX1

Homo sapiens

UniProt P62877

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 8 ubiquitin-conjugating enzyme E2 D2 isoform X3 × 1 （A0A6P5C4T4） NEDD8 × 1 （Q15843） Ubiquitin × 1 （P0CG48） DNA excision repair protein ERCC-8 × 1 （Q13216） UV-stimulated scaffold protein A × 1 （Q2YD98） DNA damage-binding protein 1 × 1 （Q16531） Cullin-4A × 1 （Q13619） ZINC ION × 3 Consistent with protein count

Other States of the Same Protein in the Database

Monomer
2lgv

1 assemblies

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
1ldj 1ldk 1ldk 1u6g 29hg 29hh 29hi 2hye 3dpl 3dqv 3dqv 3dqv

107 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name RBX1_HUMAN
Isoform —
PDB entities 3
Chains and sequence ranges Author chain R； PDBConstruct 1–108； UniProt 1–108

Ubiquitin

Homo sapiens

UniProt P0CG48

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 8 ubiquitin-conjugating enzyme E2 D2 isoform X3 × 1 （A0A6P5C4T4） NEDD8 × 1 （Q15843） E3 ubiquitin-protein ligase RBX1 × 1 （P62877） DNA excision repair protein ERCC-8 × 1 （Q13216） UV-stimulated scaffold protein A × 1 （Q2YD98） DNA damage-binding protein 1 × 1 （Q16531） Cullin-4A × 1 （Q13619） ZINC ION × 3 Consistent with protein count

Other States of the Same Protein in the Database

Monomer
1ogw 2l3z 2ld9 2mi8 2mor 2rsu 2ru6 4boz 4dhj 4dhj 4dhz 4hk2

52 assemblies; showing the first 12

Homodimer
2mbo 2mbq 2n2k 3aul 4zqs

5 assemblies

Homotetramer
3alb 8e7o

2 assemblies

Other homooligomers

None

Heteromeric or nucleic-acid complex
11sy 2lvo 2lvp 2lvq 2lz6 2mcn 2mj5 2mre 2mws 2rr9 3b08 3b08

290 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name UBC_HUMAN
Isoform —
PDB entities 4
Chains and sequence ranges Author chain U； PDBConstruct 1–76； UniProt 1–76

DNA excision repair protein ERCC-8

Homo sapiens

UniProt Q13216

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 8 ubiquitin-conjugating enzyme E2 D2 isoform X3 × 1 （A0A6P5C4T4） NEDD8 × 1 （Q15843） E3 ubiquitin-protein ligase RBX1 × 1 （P62877） Ubiquitin × 1 （P0CG48） UV-stimulated scaffold protein A × 1 （Q2YD98） DNA damage-binding protein 1 × 1 （Q16531） Cullin-4A × 1 （Q13619） ZINC ION × 3 Consistent with protein count

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
4a11 6fcv 7oo3 7oob 7oop 7opc 7opd 8b3d 8b3f 8b3g 8b3i 8qh5

16 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name ERCC8_HUMAN
Isoform —
PDB entities 5
Chains and sequence ranges Author chain a； PDBConstruct 1–396； UniProt 1–396

UV-stimulated scaffold protein A

Homo sapiens

UniProt Q2YD98

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 8 ubiquitin-conjugating enzyme E2 D2 isoform X3 × 1 （A0A6P5C4T4） NEDD8 × 1 （Q15843） E3 ubiquitin-protein ligase RBX1 × 1 （P62877） Ubiquitin × 1 （P0CG48） DNA excision repair protein ERCC-8 × 1 （Q13216） DNA damage-binding protein 1 × 1 （Q16531） Cullin-4A × 1 （Q13619） ZINC ION × 3 Consistent with protein count

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
5xv8 7oo3 7oop 7opc 7opd 8b3d 8b3g 8qh5 9bz0 9er2 9fd2 9hwg

12 assemblies

View Construct and Data Evidence
UniProt name UVSSA_HUMAN
Isoform —
PDB entities 6
Chains and sequence ranges Author chain c； PDBConstruct 1–709； UniProt 1–709

DNA damage-binding protein 1

Homo sapiens

UniProt Q16531

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 8 ubiquitin-conjugating enzyme E2 D2 isoform X3 × 1 （A0A6P5C4T4） NEDD8 × 1 （Q15843） E3 ubiquitin-protein ligase RBX1 × 1 （P62877） Ubiquitin × 1 （P0CG48） DNA excision repair protein ERCC-8 × 1 （Q13216） UV-stimulated scaffold protein A × 1 （Q2YD98） Cullin-4A × 1 （Q13619） ZINC ION × 3 Consistent with protein count

Other States of the Same Protein in the Database

Monomer
3e0c 8zsw 9bbe 9bbg 9bbh 9bbi 9ejq 9zxm 9zxn

9 assemblies

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
10ay 2hye 3ei1 3ei2 3ei3 3ei4 3ei4 3ei4 3i7h 3i7k 3i7l 3i7n

282 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name DDB1_HUMAN
Isoform —
PDB entities 7
Chains and sequence ranges Author chain d； PDBConstruct 1–1140； UniProt 1–1140

Cullin-4A

Homo sapiens

UniProt Q13619

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 8 ubiquitin-conjugating enzyme E2 D2 isoform X3 × 1 （A0A6P5C4T4） NEDD8 × 1 （Q15843） E3 ubiquitin-protein ligase RBX1 × 1 （P62877） Ubiquitin × 1 （P0CG48） DNA excision repair protein ERCC-8 × 1 （Q13216） UV-stimulated scaffold protein A × 1 （Q2YD98） DNA damage-binding protein 1 × 1 （Q16531） ZINC ION × 3 Consistent with protein count

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
2hye 4a0k 7okq 7opc 7opd 8b3g 8b3i 9eg8 9lwi 9lwj 9lwl 9pvh

16 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name CUL4A_HUMAN
Isoform —
PDB entities 8
Chains and sequence ranges Author chain e； PDBConstruct 1–759； UniProt 1–759

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id8b3g
Deposition date deposition_date2022-09-16
Structure title titleC(N)RL4CSA-UVSSA-E2-ubiquitin complex.
Keywords keywordstranscription, DNA repair, ubiquitin, cryo-EM; TRANSCRIPTION
Experimental Method methodELECTRON MICROSCOPY
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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

8b3g__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

8b3g__assembly_1__model_1 | I(q)

10-2 10-1 106 107 108 109 q (1/Angstrom) I(q)
100 plotted points; both axes use logarithmic scales.

8b3g__assembly_1__model_1 | P(r) · Pending

The new assembly/model P(r) has not been calculated yet This placeholder does not display legacy data r (Angstrom) P(r)
P(r) will be calculated and displayed separately for the same assembly/model.
Rg（Guinier）53.57 Å
Rg (electron density)53.46 Å
Total Rg53.61 Å
Atom count42828
Residues2685
Excluded volume382290 ų
Maximum q0.500 Å⁻¹
Assembly Model Structure unit Oligomeric description Status CRYSOL Actions
1 1 8b3g__assembly_1__model_1 octameric （8） Success 4.1.3-1-20251215 (887e7ef) View Download
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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (9)

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7. Citations (1)