← Back to search
8eoi

Structure of a human EMC:human Cav1.2 channel complex in GDN detergent

Method: ELECTRON MICROSCOPY Dmax: 230.8 Å Quality: GOOD
▼

1. Protein Identity and Related Structures Protein Identity & Related Structures

ER membrane protein complex subunit 1

Homo sapiens

UniProt Q8N766

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Other combination Heteromer Protein × 11 其他Polymer 3 PDB declaration: undecameric（11） Consistent with protein copy count Chain A； UniProt 21–993 Not recorded ER membrane protein complex subunit 2 × 1 （Q15006） ER membrane protein complex subunit 3 × 1 （Q9P0I2） ER membrane protein complex subunit 5 × 1 （Q8N4V1） ER membrane protein complex subunit 6 × 1 （Q9BV81） ER membrane protein complex subunit 7 × 1 （Q9NPA0） ER membrane protein complex subunit 8 × 1 （O43402） ER membrane protein complex subunit 10 × 1 （Q5UCC4） Voltage-dependent L-type calcium channel subunit alpha-1C × 1 （Q13936） Voltage-dependent L-type calcium channel subunit beta-3 × 1 （P54286） ER membrane protein complex subunit 4 × 1 （Q5J8M3） 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 3 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 9Z9 (3beta,14beta,17beta,25R)-3-[4-methoxy-3-(methoxymethyl)butoxy]spirost-5-en × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 8 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.40 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8EOI Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
6WW7 Structure of the human ER membrane protein complex in a lipid nanodisc Deposited 2020-05-08 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain A 1–993（993 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
6Z3W Human ER membrane protein complex Deposited 2020-05-22 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 9 PDB declaration: nonameric
Chain A 482–510（29 aa）
Chain A 960–993（34 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.40 Å
7ADO Cryo-EM structure of human ER membrane protein complex in lipid nanodiscs Deposited 2020-09-15 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric
Chain A 1–993（993 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 4 PCW 1,2-DIOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 6；10 mM ammonium citrate pH 6.0, 100 mM sodium chloride, 0.25 mM TCEP
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.39 Å
7ADP Cryo-EM structure of human ER membrane protein complex in GDN detergent Deposited 2020-09-15 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–993（993 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 6；10 mM ammonium citrate pH 6.0, 100 mM sodium chloride, 0.25 mM TCEP
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8J0N cryo-EM structure of human EMC Deposited 2023-04-11 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain A 1–993（993 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.47 Å
8J0O cryo-EM structure of human EMC and VDAC Deposited 2023-04-11 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 10 PDB declaration: decameric
Chain A 1–993（993 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.32 Å
8S9S Structure of the human ER membrane protein complex (EMC) in GDN Deposited 2023-03-30 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain 1 1–993（993 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 PCW 1,2-DIOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
9C7V Structure of the human BOS:human EMC complex in GDN Deposited 2024-06-11 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 12 PDB declaration: dodecameric
Chain 1 1–993（993 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.60 Å
9ZZ6 The ER membrane protein complex acts as a chaperone to promote voltage-gated calcium channel assembly Deposited 2026-01-06 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 11 PDB declaration: undecameric
Chain A 1–993（993 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；pH 7.5 adjusted with KOH ( potassium hydroxide).
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.16 Å

9 other PDB entries and 9 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name EMC1_HUMAN
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 1–973； UniProt 21–993

ER membrane protein complex subunit 2

Homo sapiens

UniProt Q15006

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Other combination Heteromer Protein × 11 其他Polymer 3 PDB declaration: undecameric（11） Consistent with protein copy count Chain B； UniProt 3–293 Not recorded ER membrane protein complex subunit 1 × 1 （Q8N766） ER membrane protein complex subunit 3 × 1 （Q9P0I2） ER membrane protein complex subunit 5 × 1 （Q8N4V1） ER membrane protein complex subunit 6 × 1 （Q9BV81） ER membrane protein complex subunit 7 × 1 （Q9NPA0） ER membrane protein complex subunit 8 × 1 （O43402） ER membrane protein complex subunit 10 × 1 （Q5UCC4） Voltage-dependent L-type calcium channel subunit alpha-1C × 1 （Q13936） Voltage-dependent L-type calcium channel subunit beta-3 × 1 （P54286） ER membrane protein complex subunit 4 × 1 （Q5J8M3） 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 3 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 9Z9 (3beta,14beta,17beta,25R)-3-[4-methoxy-3-(methoxymethyl)butoxy]spirost-5-en × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 8 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.40 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8EOI Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
6WW7 Structure of the human ER membrane protein complex in a lipid nanodisc Deposited 2020-05-08 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain B 1–297（297 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
6Y4L Crystal structure of human ER membrane protein complex subunits EMC2 and EMC9 Deposited 2020-02-21 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 11–274（264 aa）
Not recorded SO4 SULFATE ION × 3 EDO 1,2-ETHANEDIOL × 3 PE4 2-{2-[2-(2-{2-[2-(2-ETHOXY-ETHOXY)-ETHOXY]-ETHOXY}-ETHOXY)-ETHOXY]-ETHOXY}-ETHANOL × 1 PEG DI(HYDROXYETHYL)ETHER × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.3；293.15 K；34% PEG4000, 0.1 M Tris pH 8.3, and 0.2 M lithium sulfate (For I03 native dataset)
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.3；293.15 K；28% PEG4000, 0.1 M Tris pH 8.3, and 0.2 M lithium sulfate (For I23 S-SAD dataset)
Resolution 2.20 Å R-free 0.250
6Z3W Human ER membrane protein complex Deposited 2020-05-22 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 9 PDB declaration: nonameric
Chain B 1–297（297 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.40 Å
7ADO Cryo-EM structure of human ER membrane protein complex in lipid nanodiscs Deposited 2020-09-15 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric
Chain B 1–297（297 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 4 PCW 1,2-DIOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 6；10 mM ammonium citrate pH 6.0, 100 mM sodium chloride, 0.25 mM TCEP
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.39 Å
7ADP Cryo-EM structure of human ER membrane protein complex in GDN detergent Deposited 2020-09-15 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain B 1–297（297 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 6；10 mM ammonium citrate pH 6.0, 100 mM sodium chloride, 0.25 mM TCEP
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8J0N cryo-EM structure of human EMC Deposited 2023-04-11 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain B 1–297（297 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.47 Å
8J0O cryo-EM structure of human EMC and VDAC Deposited 2023-04-11 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 10 PDB declaration: decameric
Chain B 1–297（297 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.32 Å
8S9S Structure of the human ER membrane protein complex (EMC) in GDN Deposited 2023-03-30 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain 2 1–297（297 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 PCW 1,2-DIOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
9C7V Structure of the human BOS:human EMC complex in GDN Deposited 2024-06-11 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 12 PDB declaration: dodecameric
Chain 2 1–297（297 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.60 Å
9ZZ6 The ER membrane protein complex acts as a chaperone to promote voltage-gated calcium channel assembly Deposited 2026-01-06 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 11 PDB declaration: undecameric
Chain B 1–297（297 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；pH 7.5 adjusted with KOH ( potassium hydroxide).
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.16 Å

10 other PDB entries and 10 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name EMC2_HUMAN
Isoform —
PDB entities 2
Chains and sequence ranges Author chain B； PDBConstruct 1–291； UniProt 3–293

ER membrane protein complex subunit 3

Homo sapiens

UniProt Q9P0I2

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Other combination Heteromer Protein × 11 其他Polymer 3 PDB declaration: undecameric（11） Consistent with protein copy count Chain C； UniProt 4–261 Not recorded ER membrane protein complex subunit 1 × 1 （Q8N766） ER membrane protein complex subunit 2 × 1 （Q15006） ER membrane protein complex subunit 5 × 1 （Q8N4V1） ER membrane protein complex subunit 6 × 1 （Q9BV81） ER membrane protein complex subunit 7 × 1 （Q9NPA0） ER membrane protein complex subunit 8 × 1 （O43402） ER membrane protein complex subunit 10 × 1 （Q5UCC4） Voltage-dependent L-type calcium channel subunit alpha-1C × 1 （Q13936） Voltage-dependent L-type calcium channel subunit beta-3 × 1 （P54286） ER membrane protein complex subunit 4 × 1 （Q5J8M3） 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 3 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 9Z9 (3beta,14beta,17beta,25R)-3-[4-methoxy-3-(methoxymethyl)butoxy]spirost-5-en × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 8 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.40 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8EOI Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
6WW7 Structure of the human ER membrane protein complex in a lipid nanodisc Deposited 2020-05-08 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain C 1–261（261 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
6Z3W Human ER membrane protein complex Deposited 2020-05-22 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 9 PDB declaration: nonameric
Chain C 1–261（261 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.40 Å
7ADO Cryo-EM structure of human ER membrane protein complex in lipid nanodiscs Deposited 2020-09-15 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric
Chain C 1–261（261 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 4 PCW 1,2-DIOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 6；10 mM ammonium citrate pH 6.0, 100 mM sodium chloride, 0.25 mM TCEP
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.39 Å
7ADP Cryo-EM structure of human ER membrane protein complex in GDN detergent Deposited 2020-09-15 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain C 1–261（261 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 6；10 mM ammonium citrate pH 6.0, 100 mM sodium chloride, 0.25 mM TCEP
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8J0N cryo-EM structure of human EMC Deposited 2023-04-11 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain C 1–261（261 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.47 Å
8J0O cryo-EM structure of human EMC and VDAC Deposited 2023-04-11 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 10 PDB declaration: decameric
Chain C 1–261（261 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.32 Å
8S9S Structure of the human ER membrane protein complex (EMC) in GDN Deposited 2023-03-30 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain 3 1–261（261 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 PCW 1,2-DIOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
9C7V Structure of the human BOS:human EMC complex in GDN Deposited 2024-06-11 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 12 PDB declaration: dodecameric
Chain 3 1–261（261 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.60 Å
9ZZ6 The ER membrane protein complex acts as a chaperone to promote voltage-gated calcium channel assembly Deposited 2026-01-06 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 11 PDB declaration: undecameric
Chain C 1–261（261 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；pH 7.5 adjusted with KOH ( potassium hydroxide).
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.16 Å

9 other PDB entries and 9 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name EMC3_HUMAN
Isoform —
PDB entities 3
Chains and sequence ranges Author chain C； PDBConstruct 1–258； UniProt 4–261

ER membrane protein complex subunit 5

Homo sapiens

UniProt Q8N4V1

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Other combination Heteromer Protein × 11 其他Polymer 3 PDB declaration: undecameric（11） Consistent with protein copy count Chain E； UniProt 3–103 Not recorded ER membrane protein complex subunit 1 × 1 （Q8N766） ER membrane protein complex subunit 2 × 1 （Q15006） ER membrane protein complex subunit 3 × 1 （Q9P0I2） ER membrane protein complex subunit 6 × 1 （Q9BV81） ER membrane protein complex subunit 7 × 1 （Q9NPA0） ER membrane protein complex subunit 8 × 1 （O43402） ER membrane protein complex subunit 10 × 1 （Q5UCC4） Voltage-dependent L-type calcium channel subunit alpha-1C × 1 （Q13936） Voltage-dependent L-type calcium channel subunit beta-3 × 1 （P54286） ER membrane protein complex subunit 4 × 1 （Q5J8M3） 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 3 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 9Z9 (3beta,14beta,17beta,25R)-3-[4-methoxy-3-(methoxymethyl)butoxy]spirost-5-en × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 8 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.40 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8EOI Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
6WW7 Structure of the human ER membrane protein complex in a lipid nanodisc Deposited 2020-05-08 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain E 66–196（131 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
6Z3W Human ER membrane protein complex Deposited 2020-05-22 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 9 PDB declaration: nonameric
Chain E 1–131（131 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.40 Å
7ADO Cryo-EM structure of human ER membrane protein complex in lipid nanodiscs Deposited 2020-09-15 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric
Chain E 1–131（131 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 4 PCW 1,2-DIOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 6；10 mM ammonium citrate pH 6.0, 100 mM sodium chloride, 0.25 mM TCEP
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.39 Å
7ADP Cryo-EM structure of human ER membrane protein complex in GDN detergent Deposited 2020-09-15 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain E 1–131（131 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 6；10 mM ammonium citrate pH 6.0, 100 mM sodium chloride, 0.25 mM TCEP
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8J0N cryo-EM structure of human EMC Deposited 2023-04-11 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain E 1–131（131 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.47 Å
8J0O cryo-EM structure of human EMC and VDAC Deposited 2023-04-11 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 10 PDB declaration: decameric
Chain E 1–131（131 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.32 Å
8S9S Structure of the human ER membrane protein complex (EMC) in GDN Deposited 2023-03-30 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain 5 66–196（131 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 PCW 1,2-DIOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
9C7V Structure of the human BOS:human EMC complex in GDN Deposited 2024-06-11 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 12 PDB declaration: dodecameric
Chain 5 66–196（131 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.60 Å
9ZZ6 The ER membrane protein complex acts as a chaperone to promote voltage-gated calcium channel assembly Deposited 2026-01-06 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 11 PDB declaration: undecameric
Chain E 66–196（131 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；pH 7.5 adjusted with KOH ( potassium hydroxide).
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.16 Å

9 other PDB entries and 9 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name EMC5_HUMAN
Isoform —
PDB entities 4
Chains and sequence ranges Author chain E； PDBConstruct 1–101； UniProt 3–103

ER membrane protein complex subunit 6

Homo sapiens

UniProt Q9BV81

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Other combination Heteromer Protein × 11 其他Polymer 3 PDB declaration: undecameric（11） Consistent with protein copy count Chain F； UniProt 11–110 Not recorded ER membrane protein complex subunit 1 × 1 （Q8N766） ER membrane protein complex subunit 2 × 1 （Q15006） ER membrane protein complex subunit 3 × 1 （Q9P0I2） ER membrane protein complex subunit 5 × 1 （Q8N4V1） ER membrane protein complex subunit 7 × 1 （Q9NPA0） ER membrane protein complex subunit 8 × 1 （O43402） ER membrane protein complex subunit 10 × 1 （Q5UCC4） Voltage-dependent L-type calcium channel subunit alpha-1C × 1 （Q13936） Voltage-dependent L-type calcium channel subunit beta-3 × 1 （P54286） ER membrane protein complex subunit 4 × 1 （Q5J8M3） 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 3 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 9Z9 (3beta,14beta,17beta,25R)-3-[4-methoxy-3-(methoxymethyl)butoxy]spirost-5-en × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 8 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.40 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8EOI Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
6WW7 Structure of the human ER membrane protein complex in a lipid nanodisc Deposited 2020-05-08 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain F 1–110（110 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
6Z3W Human ER membrane protein complex Deposited 2020-05-22 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 9 PDB declaration: nonameric
Chain F 1–110（110 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.40 Å
7ADO Cryo-EM structure of human ER membrane protein complex in lipid nanodiscs Deposited 2020-09-15 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric
Chain F 1–110（110 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 4 PCW 1,2-DIOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 6；10 mM ammonium citrate pH 6.0, 100 mM sodium chloride, 0.25 mM TCEP
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.39 Å
7ADP Cryo-EM structure of human ER membrane protein complex in GDN detergent Deposited 2020-09-15 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain F 1–110（110 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 6；10 mM ammonium citrate pH 6.0, 100 mM sodium chloride, 0.25 mM TCEP
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8J0N cryo-EM structure of human EMC Deposited 2023-04-11 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain F 1–110（110 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.47 Å
8J0O cryo-EM structure of human EMC and VDAC Deposited 2023-04-11 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 10 PDB declaration: decameric
Chain F 1–110（110 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.32 Å
8S9S Structure of the human ER membrane protein complex (EMC) in GDN Deposited 2023-03-30 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain 6 1–110（110 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 PCW 1,2-DIOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
9C7V Structure of the human BOS:human EMC complex in GDN Deposited 2024-06-11 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 12 PDB declaration: dodecameric
Chain 6 1–110（110 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.60 Å
9ZZ6 The ER membrane protein complex acts as a chaperone to promote voltage-gated calcium channel assembly Deposited 2026-01-06 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 11 PDB declaration: undecameric
Chain F 1–110（110 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；pH 7.5 adjusted with KOH ( potassium hydroxide).
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.16 Å

9 other PDB entries and 9 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name EMC6_HUMAN
Isoform —
PDB entities 5
Chains and sequence ranges Author chain F； PDBConstruct 1–100； UniProt 11–110

ER membrane protein complex subunit 7

Homo sapiens

UniProt Q9NPA0

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Other combination Heteromer Protein × 11 其他Polymer 3 PDB declaration: undecameric（11） Consistent with protein copy count Chain G； UniProt 43–158 Not recorded ER membrane protein complex subunit 1 × 1 （Q8N766） ER membrane protein complex subunit 2 × 1 （Q15006） ER membrane protein complex subunit 3 × 1 （Q9P0I2） ER membrane protein complex subunit 5 × 1 （Q8N4V1） ER membrane protein complex subunit 6 × 1 （Q9BV81） ER membrane protein complex subunit 8 × 1 （O43402） ER membrane protein complex subunit 10 × 1 （Q5UCC4） Voltage-dependent L-type calcium channel subunit alpha-1C × 1 （Q13936） Voltage-dependent L-type calcium channel subunit beta-3 × 1 （P54286） ER membrane protein complex subunit 4 × 1 （Q5J8M3） 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 3 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 9Z9 (3beta,14beta,17beta,25R)-3-[4-methoxy-3-(methoxymethyl)butoxy]spirost-5-en × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 8 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.40 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8EOI Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
2UWE Large CDR3a loop alteration as a function of MHC mutation Deposited 2007-03-20 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain C 4–12（9 aa） Fragment：RESIDUES 4-12
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.8；14% PEG 8000, 1 M NACL, 25 MM HEPES, PH 7.8
Resolution 2.40 Å R-free 0.290
2UWE Large CDR3a loop alteration as a function of MHC mutation Deposited 2007-03-20 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain J 4–12（9 aa） Fragment：RESIDUES 4-12
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.8；14% PEG 8000, 1 M NACL, 25 MM HEPES, PH 7.8
Resolution 2.40 Å R-free 0.290
6WW7 Structure of the human ER membrane protein complex in a lipid nanodisc Deposited 2020-05-08 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain G 1–242（242 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
6Z3W Human ER membrane protein complex Deposited 2020-05-22 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 9 PDB declaration: nonameric
Chain G 1–242（242 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.40 Å
7ADO Cryo-EM structure of human ER membrane protein complex in lipid nanodiscs Deposited 2020-09-15 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric
Chain G 1–242（242 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 4 PCW 1,2-DIOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 6；10 mM ammonium citrate pH 6.0, 100 mM sodium chloride, 0.25 mM TCEP
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.39 Å
8J0N cryo-EM structure of human EMC Deposited 2023-04-11 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain G 1–242（242 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.47 Å
8J0O cryo-EM structure of human EMC and VDAC Deposited 2023-04-11 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 10 PDB declaration: decameric
Chain G 1–242（242 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.32 Å
8S9S Structure of the human ER membrane protein complex (EMC) in GDN Deposited 2023-03-30 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain 7 1–242（242 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 PCW 1,2-DIOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
9C7V Structure of the human BOS:human EMC complex in GDN Deposited 2024-06-11 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 12 PDB declaration: dodecameric
Chain 7 1–242（242 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.60 Å
9ZZ6 The ER membrane protein complex acts as a chaperone to promote voltage-gated calcium channel assembly Deposited 2026-01-06 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 11 PDB declaration: undecameric
Chain G 1–242（242 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；pH 7.5 adjusted with KOH ( potassium hydroxide).
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.16 Å

9 other PDB entries and 10 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name EMC7_HUMAN
Isoform —
PDB entities 6
Chains and sequence ranges Author chain G； PDBConstruct 1–116； UniProt 43–158

ER membrane protein complex subunit 8

Homo sapiens

UniProt O43402

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Other combination Heteromer Protein × 11 其他Polymer 3 PDB declaration: undecameric（11） Consistent with protein copy count Chain H； UniProt 3–210 Not recorded ER membrane protein complex subunit 1 × 1 （Q8N766） ER membrane protein complex subunit 2 × 1 （Q15006） ER membrane protein complex subunit 3 × 1 （Q9P0I2） ER membrane protein complex subunit 5 × 1 （Q8N4V1） ER membrane protein complex subunit 6 × 1 （Q9BV81） ER membrane protein complex subunit 7 × 1 （Q9NPA0） ER membrane protein complex subunit 10 × 1 （Q5UCC4） Voltage-dependent L-type calcium channel subunit alpha-1C × 1 （Q13936） Voltage-dependent L-type calcium channel subunit beta-3 × 1 （P54286） ER membrane protein complex subunit 4 × 1 （Q5J8M3） 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 3 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 9Z9 (3beta,14beta,17beta,25R)-3-[4-methoxy-3-(methoxymethyl)butoxy]spirost-5-en × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 8 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.40 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8EOI Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
6WW7 Structure of the human ER membrane protein complex in a lipid nanodisc Deposited 2020-05-08 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain H 1–210（210 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
7ADO Cryo-EM structure of human ER membrane protein complex in lipid nanodiscs Deposited 2020-09-15 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric
Chain H 1–210（210 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 4 PCW 1,2-DIOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 6；10 mM ammonium citrate pH 6.0, 100 mM sodium chloride, 0.25 mM TCEP
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.39 Å
7ADP Cryo-EM structure of human ER membrane protein complex in GDN detergent Deposited 2020-09-15 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain H 1–210（210 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 6；10 mM ammonium citrate pH 6.0, 100 mM sodium chloride, 0.25 mM TCEP
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8J0N cryo-EM structure of human EMC Deposited 2023-04-11 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain H 1–210（210 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.47 Å
8J0O cryo-EM structure of human EMC and VDAC Deposited 2023-04-11 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 10 PDB declaration: decameric
Chain H 1–210（210 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.32 Å
8S9S Structure of the human ER membrane protein complex (EMC) in GDN Deposited 2023-03-30 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain 8 1–210（210 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 PCW 1,2-DIOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
9C7V Structure of the human BOS:human EMC complex in GDN Deposited 2024-06-11 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 12 PDB declaration: dodecameric
Chain 8 1–210（210 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.60 Å
9ZZ6 The ER membrane protein complex acts as a chaperone to promote voltage-gated calcium channel assembly Deposited 2026-01-06 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 11 PDB declaration: undecameric
Chain H 1–210（210 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；pH 7.5 adjusted with KOH ( potassium hydroxide).
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.16 Å

8 other PDB entries and 8 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name EMC8_HUMAN
Isoform —
PDB entities 7
Chains and sequence ranges Author chain H； PDBConstruct 1–208； UniProt 3–210

ER membrane protein complex subunit 10

Homo sapiens

UniProt Q5UCC4

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Other combination Heteromer Protein × 11 其他Polymer 3 PDB declaration: undecameric（11） Consistent with protein copy count Chain I； UniProt 50–206 Not recorded ER membrane protein complex subunit 1 × 1 （Q8N766） ER membrane protein complex subunit 2 × 1 （Q15006） ER membrane protein complex subunit 3 × 1 （Q9P0I2） ER membrane protein complex subunit 5 × 1 （Q8N4V1） ER membrane protein complex subunit 6 × 1 （Q9BV81） ER membrane protein complex subunit 7 × 1 （Q9NPA0） ER membrane protein complex subunit 8 × 1 （O43402） Voltage-dependent L-type calcium channel subunit alpha-1C × 1 （Q13936） Voltage-dependent L-type calcium channel subunit beta-3 × 1 （P54286） ER membrane protein complex subunit 4 × 1 （Q5J8M3） 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 3 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 9Z9 (3beta,14beta,17beta,25R)-3-[4-methoxy-3-(methoxymethyl)butoxy]spirost-5-en × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 8 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.40 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8EOI Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
6WW7 Structure of the human ER membrane protein complex in a lipid nanodisc Deposited 2020-05-08 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain I 1–262（262 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
6Z3W Human ER membrane protein complex Deposited 2020-05-22 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 9 PDB declaration: nonameric
Chain I 1–262（262 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.40 Å
7ADO Cryo-EM structure of human ER membrane protein complex in lipid nanodiscs Deposited 2020-09-15 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric
Chain I 2–262（261 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 4 PCW 1,2-DIOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 6；10 mM ammonium citrate pH 6.0, 100 mM sodium chloride, 0.25 mM TCEP
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.39 Å
7ADP Cryo-EM structure of human ER membrane protein complex in GDN detergent Deposited 2020-09-15 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain I 2–262（261 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 6；10 mM ammonium citrate pH 6.0, 100 mM sodium chloride, 0.25 mM TCEP
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8J0N cryo-EM structure of human EMC Deposited 2023-04-11 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain J 1–207（207 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.47 Å
8J0O cryo-EM structure of human EMC and VDAC Deposited 2023-04-11 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 10 PDB declaration: decameric
Chain J 1–207（207 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.32 Å
8S9S Structure of the human ER membrane protein complex (EMC) in GDN Deposited 2023-03-30 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain 10 1–262（262 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 PCW 1,2-DIOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
9C7V Structure of the human BOS:human EMC complex in GDN Deposited 2024-06-11 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 12 PDB declaration: dodecameric
Chain 10 1–262（262 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.60 Å
9ZZ6 The ER membrane protein complex acts as a chaperone to promote voltage-gated calcium channel assembly Deposited 2026-01-06 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 11 PDB declaration: undecameric
Chain I 1–262（262 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；pH 7.5 adjusted with KOH ( potassium hydroxide).
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.16 Å

9 other PDB entries and 9 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name EMC10_HUMAN
Isoform —
PDB entities 8
Chains and sequence ranges Author chain I； PDBConstruct 1–157； UniProt 50–206

Voltage-dependent L-type calcium channel subunit alpha-1C

Homo sapiens

UniProt Q13936

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Other combination Heteromer Protein × 11 其他Polymer 3 PDB declaration: undecameric（11） Consistent with protein copy count Chain K； UniProt 109–1658 Not recorded ER membrane protein complex subunit 1 × 1 （Q8N766） ER membrane protein complex subunit 2 × 1 （Q15006） ER membrane protein complex subunit 3 × 1 （Q9P0I2） ER membrane protein complex subunit 5 × 1 （Q8N4V1） ER membrane protein complex subunit 6 × 1 （Q9BV81） ER membrane protein complex subunit 7 × 1 （Q9NPA0） ER membrane protein complex subunit 8 × 1 （O43402） ER membrane protein complex subunit 10 × 1 （Q5UCC4） Voltage-dependent L-type calcium channel subunit beta-3 × 1 （P54286） ER membrane protein complex subunit 4 × 1 （Q5J8M3） 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 3 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 9Z9 (3beta,14beta,17beta,25R)-3-[4-methoxy-3-(methoxymethyl)butoxy]spirost-5-en × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 8 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.40 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8EOI Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
1T0J Crystal structure of a complex between voltage-gated calcium channel beta2a subunit and a peptide of the alpha1c subunit Deposited 2004-04-09 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain C 428–445（18 aa） Fragment：Residues 428-443
Not recorded CL CHLORIDE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；Tris-Cl, PEG 4000, NaCl, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.00 Å R-free 0.242
1T0J Crystal structure of a complex between voltage-gated calcium channel beta2a subunit and a peptide of the alpha1c subunit Deposited 2004-04-09 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain C 428–445（18 aa） Fragment：Residues 428-443
Not recorded CL CHLORIDE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；Tris-Cl, PEG 4000, NaCl, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.00 Å R-free 0.242
2LQC NMR solution structure of a Ca2+-Calmodulin with a binding motif (NSCaTE) peptide from the N-terminal cytoplasmic domain of the L-type Voltage-Cated Calcium Channel alpha1C subunit Deposited 2012-02-29 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 47–68（22 aa） Fragment：UNP residues 47-68
Not recorded CA CALCIUM ION × 2 SOLUTION NMR
NMR measurement conditions pH 7；298 K；Ionic strength (raw mmCIF value) 100；Pressure ambient
NMR sample composition 1 mM [U-100% 13C; U-100% 15N] calmodulin, 2 mM NSCaTE peptide, 55 M H2O, 5 M [U-99% 2H] D2O, 10 mM DTT, 20 mM TRIS, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 1 mM [U-100% 13C; U-100% 15N] NSCaTE peptide, 0.5 mM calmodulin, 55 M H2O, 5 M [U-99% 2H] D2O, 10 mM DTT, 20 mM TRIS, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 1 mM [U-100% 13C; U-100% 15N] calmodulin, 2 mM NSCaTE peptide, 7.5 mg Pf1 phage, 90% H2O/10% D2O | 90% H2O/10% D2O
Resolution not provided
3G43 Crystal structure of the calmodulin-bound Cav1.2 C-terminal regulatory domain dimer Deposited 2009-02-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain E 1609–1682（74 aa） Fragment：C-terminal fragment: UNP residues 1609-1682
Chain F 1609–1682（74 aa） Fragment：C-terminal fragment: UNP residues 1609-1682
Not recorded CA CALCIUM ION × 14 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；298 K；20% PEG 6000, 1.0 M LiCl, 3% w/v MPD, 100 mM MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.10 Å R-free 0.269
3OXQ Crystal Structure of Ca2+/CaM-CaV1.2 pre-IQ/IQ domain complex Deposited 2010-09-21 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain E 1609–1685（77 aa） Fragment：preIQ/IQ domain (unp residues 1609-1685)
Not recorded CA CALCIUM ION × 7 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.5；293 K；0.2 M Li2SO4, 24.5% PEG3350, 0.1 M Bis-Tris pH5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.55 Å R-free 0.288
3OXQ Crystal Structure of Ca2+/CaM-CaV1.2 pre-IQ/IQ domain complex Deposited 2010-09-21 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain F 1609–1685（77 aa） Fragment：preIQ/IQ domain (unp residues 1609-1685)
Not recorded CA CALCIUM ION × 7 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.5；293 K；0.2 M Li2SO4, 24.5% PEG3350, 0.1 M Bis-Tris pH5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.55 Å R-free 0.288
5V2P CaV beta2a subunit: CaV1.2 AID-CAP complex Deposited 2017-03-06 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 427–445（19 aa） Fragment：AID-CAP (UNP residues 427-445)
Mutation：K427C, Q428S, Q429P, E432C NI NICKEL (II) ION × 1 1PE PENTAETHYLENE GLYCOL × 2 8VY 1,3-bis(bromomethyl)benzene × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.3；277 K；34-37% PEG400, 0.1 M magnesium chloride, 0.1 M MES, pH 6.3
Resolution 2.00 Å R-free 0.230
5V2Q CaV beta2a subunit: CaV1.2 AID-CEN complex Deposited 2017-03-06 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 427–445（19 aa） Fragment：AID-CEN (UNP residues 427-445)
Mutation：K427A, Q428S, Q429P, K435C, D439C CL CHLORIDE ION × 1 8VY 1,3-bis(bromomethyl)benzene × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；1.5-1.7 M ammonium sulfate, 5 mM BME, 0.1 M HEPES, pH 7.0
Resolution 1.70 Å R-free 0.196
6C0A Actinin-1 EF-Hand bound to the Cav1.2 IQ Motif Deposited 2017-12-28 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 1587–1609（23 aa） Fragment：IQ Motif (UNP residues 1587-1609)
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 7.5；303 K；Ionic strength (raw mmCIF value) 0；Pressure 1
NMR sample composition 300 uM [U-99% 15N] Actinin-1 EF hand, 300 uM IQ Motif, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 300 uM [U-99% 15N] Cav1.2 IQ Motif, 300 uM Actinin-1 EF 3,3, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 300 uM [U-99% 13C; U-99% 15N] Actinin-1 EF 3,4, 300 uM Cav1.2 IQ Motif, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 12 mg/mL Pf1 phage, 300 uM [U-99% 15N] Actinin-1 EF 3,4, 300 uM Cav1.2 IQ Motif, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 12 mg/mL Pf1 phage, 300 uM [U-99% 15N] Cav1.2 IQ Motif, 300 uM Actinin-1 EF 3,4, 90% H2O/10% D2O | 90% H2O/10% D2O
Resolution not provided
6DAD 1.65 Angstrom crystal structure of the N97I Ca/CaM:CaV1.2 IQ domain complex Deposited 2018-05-01 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain C 1611–1644（34 aa） Fragment：UNP residues 1611-1644
Not recorded CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；298 K；2.1 M sodium malonate, 0.1 M HEPES, pH 7.5
Resolution 1.65 Å R-free 0.208
6DAD 1.65 Angstrom crystal structure of the N97I Ca/CaM:CaV1.2 IQ domain complex Deposited 2018-05-01 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain D 1611–1644（34 aa） Fragment：UNP residues 1611-1644
Not recorded CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；298 K；2.1 M sodium malonate, 0.1 M HEPES, pH 7.5
Resolution 1.65 Å R-free 0.208
6DAE 2.0 Angstrom crystal structure of the D95V Ca/CaM:CaV1.2 IQ domain complex Deposited 2018-05-01 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain C 1611–1644（34 aa） Fragment：UNP residues 1611-1644
Not recorded CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 10.3；277 K；1 M lithium chloride, 30% PEG6000, 0.1M Bicine, pH 10.3
Resolution 2.00 Å R-free 0.226
6DAE 2.0 Angstrom crystal structure of the D95V Ca/CaM:CaV1.2 IQ domain complex Deposited 2018-05-01 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain D 1611–1644（34 aa） Fragment：UNP residues 1611-1644
Not recorded CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 10.3；277 K；1 M lithium chloride, 30% PEG6000, 0.1M Bicine, pH 10.3
Resolution 2.00 Å R-free 0.226
6DAF 2.4 Angstrom crystal structure of the F141L Ca/CaM:CaV1.2 IQ domain complex Deposited 2018-05-01 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain C 1611–1644（34 aa） Fragment：UNP residues 1611-1644
Not recorded CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；298 K；0.5 M potassium nitrate, 20% PEG3350
Resolution 2.40 Å R-free 0.243
6DAF 2.4 Angstrom crystal structure of the F141L Ca/CaM:CaV1.2 IQ domain complex Deposited 2018-05-01 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain D 1611–1644（34 aa） Fragment：UNP residues 1611-1644
Not recorded CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；298 K；0.5 M potassium nitrate, 20% PEG3350
Resolution 2.40 Å R-free 0.243
6U39 2.4 Angstrom crystal structure of the D129G Ca-CaM:CaV1.2 IQ domain complex Deposited 2019-08-21 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 1611–1644（34 aa）
Not recorded CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；298 K；ammonium sulfate
Resolution 2.40 Å R-free 0.283
6U39 2.4 Angstrom crystal structure of the D129G Ca-CaM:CaV1.2 IQ domain complex Deposited 2019-08-21 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 10 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain T 1611–1644（34 aa）
Not recorded CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；298 K；ammonium sulfate
Resolution 2.40 Å R-free 0.283
6U39 2.4 Angstrom crystal structure of the D129G Ca-CaM:CaV1.2 IQ domain complex Deposited 2019-08-21 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain D 1611–1644（34 aa）
Not recorded CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；298 K；ammonium sulfate
Resolution 2.40 Å R-free 0.283
6U39 2.4 Angstrom crystal structure of the D129G Ca-CaM:CaV1.2 IQ domain complex Deposited 2019-08-21 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain F 1611–1644（34 aa）
Not recorded CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；298 K；ammonium sulfate
Resolution 2.40 Å R-free 0.283
6U39 2.4 Angstrom crystal structure of the D129G Ca-CaM:CaV1.2 IQ domain complex Deposited 2019-08-21 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain H 1611–1644（34 aa）
Not recorded CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；298 K；ammonium sulfate
Resolution 2.40 Å R-free 0.283
6U39 2.4 Angstrom crystal structure of the D129G Ca-CaM:CaV1.2 IQ domain complex Deposited 2019-08-21 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 5 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain J 1611–1644（34 aa）
Not recorded CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；298 K；ammonium sulfate
Resolution 2.40 Å R-free 0.283
6U39 2.4 Angstrom crystal structure of the D129G Ca-CaM:CaV1.2 IQ domain complex Deposited 2019-08-21 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 6 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain L 1611–1644（34 aa）
Not recorded CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；298 K；ammonium sulfate
Resolution 2.40 Å R-free 0.283
6U39 2.4 Angstrom crystal structure of the D129G Ca-CaM:CaV1.2 IQ domain complex Deposited 2019-08-21 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 7 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain N 1611–1644（34 aa）
Not recorded CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；298 K；ammonium sulfate
Resolution 2.40 Å R-free 0.283
6U39 2.4 Angstrom crystal structure of the D129G Ca-CaM:CaV1.2 IQ domain complex Deposited 2019-08-21 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 8 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain P 1611–1644（34 aa）
Not recorded CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；298 K；ammonium sulfate
Resolution 2.40 Å R-free 0.283
6U39 2.4 Angstrom crystal structure of the D129G Ca-CaM:CaV1.2 IQ domain complex Deposited 2019-08-21 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 9 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain R 1611–1644（34 aa）
Not recorded CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；298 K；ammonium sulfate
Resolution 2.40 Å R-free 0.283
6U3A 1.65 Angstrom crystal structure of the N97S Ca-CaM:CaV1.2 IQ domain complex Deposited 2019-08-21 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain C 1611–1644（34 aa）
Not recorded CA CALCIUM ION × 3 NA SODIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；277 K；DL-malic acid
Resolution 1.65 Å R-free 0.209
6U3A 1.65 Angstrom crystal structure of the N97S Ca-CaM:CaV1.2 IQ domain complex Deposited 2019-08-21 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain D 1611–1644（34 aa）
Not recorded CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；277 K；DL-malic acid
Resolution 1.65 Å R-free 0.209
6U3B 1.7 Angstrom crystal structure of the Q135P Ca-CaM:CaV1.2 IQ domain complex Deposited 2019-08-21 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 1611–1644（34 aa）
Not recorded CA CALCIUM ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；298 K；PEG 550 MME, sodium acetate
Resolution 1.70 Å R-free 0.200
6U3D 1.75 Angstrom crystal structure of the N53I Ca-CaM:CaV1.2 IQ domain complex Deposited 2019-08-21 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain C 1611–1644（34 aa）
Not recorded CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；298 K；PEG 3350, citric acid
Resolution 1.75 Å R-free 0.201
6U3D 1.75 Angstrom crystal structure of the N53I Ca-CaM:CaV1.2 IQ domain complex Deposited 2019-08-21 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain D 1611–1644（34 aa）
Not recorded CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；298 K；PEG 3350, citric acid
Resolution 1.75 Å R-free 0.201
7L8V NMR Structure of half-calcified calmodulin mutant (CaMEF12) bound to the IQ-motif of CaV1.2 Deposited 2021-01-01 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain C 1662–1682（21 aa） Fragment：IQ-motif residues 1662-1682
Not recorded CA CALCIUM ION × 2 SOLUTION NMR
NMR measurement conditions pH 7.4；303 K；Ionic strength (raw mmCIF value) 0.1；Pressure 1
NMR sample composition 0.5 mM [U-95% 15N] calmodulin, 0.75 mM IQ-motif, 10 mM TRIS, 2 mM CALCIUM ION, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 0.5 mM [U-13C; U-15N] calmodulin, 0.75 mM IQ-motif, 10 mM TRIS, 2 mM CALCIUM ION, 90% H2O/10% D2O | 90% H2O/10% D2O
Resolution not provided
8FD7 Structure of the human L-type voltage-gated calcium channel Cav1.2 complexed with gabapentin Deposited 2022-12-02 Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 3 PDB declaration: trimeric
Chain K 1–1648（1648 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 8 GBN [1-(AMINOMETHYL)CYCLOHEXYL]ACETIC ACID × 1 CA CALCIUM ION × 3 NA SODIUM ION × 1 WO9 (2R)-3-{[(R)-(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-2-(dodecanoyloxy)propyl heptadecanoate × 1 YSW (2S)-3-{[(R)-(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-2-(dodecanoyloxy)propyl dodecanoate × 1 CLR CHOLESTEROL × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8FHS Human L-type voltage-gated calcium channel Cav1.2 in the presence of amiodarone and sofosbuvir at 3.3 Angstrom resolution Deposited 2022-12-15 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–2221（2221 aa）
Not recorded CA CALCIUM ION × 2 BBI (2-butyl-1-benzofuran-3-yl){4-[2-(diethylamino)ethoxy]-3,5-diiodophenyl}methanone × 1 WG6 Sofosbuvir × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 CLR CHOLESTEROL × 4 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8WE6 Human L-type voltage-gated calcium channel Cav1.2 at 2.9 Angstrom resolution Deposited 2023-09-17 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–2201（2201 aa）
Not recorded CA CALCIUM ION × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 CLR CHOLESTEROL × 4 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 PT5 [(2R)-1-octadecanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phospho ryl]oxy-propan-2-yl] (8Z)-icosa-5,8,11,14-tetraenoate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8WE7 Human L-type voltage-gated calcium channel Cav1.2 in the presence of calciseptine at 3.2 Angstrom resolution Deposited 2023-09-17 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 4 PDB declaration: tetrameric
Chain A 1–2201（2201 aa）
Not recorded CA CALCIUM ION × 2 CLR CHOLESTEROL × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8WE8 Human L-type voltage-gated calcium channel Cav1.2 in the presence of calciseptine, amlodipine and pinaverium at 2.9 Angstrom resolution Deposited 2023-09-17 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 4 PDB declaration: tetrameric
Chain A 1–2201（2201 aa）
Not recorded CA CALCIUM ION × 2 CLR CHOLESTEROL × 5 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 PT5 [(2R)-1-octadecanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phospho ryl]oxy-propan-2-yl] (8Z)-icosa-5,8,11,14-tetraenoate × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 6UB amlodipine × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8WE9 Human L-type voltage-gated calcium channel Cav1.2 (Class I) in the presence of pinaverium at 3.0 Angstrom resolution Deposited 2023-09-17 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–2201（2201 aa）
Not recorded CA CALCIUM ION × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 CLR CHOLESTEROL × 3 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 PT5 [(2R)-1-octadecanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phospho ryl]oxy-propan-2-yl] (8Z)-icosa-5,8,11,14-tetraenoate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8WEA Human L-type voltage-gated calcium channel Cav1.2 (Class II) in the presence of pinaverium at 3.2 Angstrom resolution Deposited 2023-09-17 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1–2201（2201 aa）
Not recorded CA CALCIUM ION × 2 9Z9 (3beta,14beta,17beta,25R)-3-[4-methoxy-3-(methoxymethyl)butoxy]spirost-5-en × 1 WB9 4-[(2-bromanyl-4,5-dimethoxy-phenyl)methyl]-4-[2-[2-[(1~{R},2~{S},5~{R})-6,6-dimethyl-2-bicyclo[3.1.1]heptanyl]ethoxy]ethyl]morpholin-4-ium × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
9ZZ4 NMR structure of CaBP1 bound to the IQ motif of Cav1.2 Deposited 2026-01-06 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 1587–1609（23 aa） Fragment：IQ Motif (UNP residues 1587-1609)
Not recorded CA CALCIUM ION × 2 SOLUTION NMR
NMR measurement conditions pH 7.5；300 K；Ionic strength (raw mmCIF value) 10；Pressure 1
NMR sample composition 0.5 mM [U-100% 15N] CaBP1 C-Lobe, 0.75 mM CaV1.2 IQ motif, 1 mM Ca2+, 10 mM [U-100% 2H] Tris, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 0.6 mM [U-100% 13C; U-100% 15N] CaBP1 C-Lobe, 0.9 mM CaV1.2 IQ motif, 1 mM Ca2+, 10 mM [U-100% 2H] Tris, 90% H2O/10% D2O | 90% H2O/10% D2O
Resolution not provided

23 other PDB entries and 39 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name CAC1C_HUMAN
Isoform —
PDB entities 9
Chains and sequence ranges Author chain K； PDBConstruct 1–1550； UniProt 109–1658

Voltage-dependent L-type calcium channel subunit beta-3

Oryctolagus cuniculus

UniProt P54286

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Other combination Heteromer Protein × 11 其他Polymer 3 PDB declaration: undecameric（11） Consistent with protein copy count Chain J； UniProt 38–361 Not recorded ER membrane protein complex subunit 1 × 1 （Q8N766） ER membrane protein complex subunit 2 × 1 （Q15006） ER membrane protein complex subunit 3 × 1 （Q9P0I2） ER membrane protein complex subunit 5 × 1 （Q8N4V1） ER membrane protein complex subunit 6 × 1 （Q9BV81） ER membrane protein complex subunit 7 × 1 （Q9NPA0） ER membrane protein complex subunit 8 × 1 （O43402） ER membrane protein complex subunit 10 × 1 （Q5UCC4） Voltage-dependent L-type calcium channel subunit alpha-1C × 1 （Q13936） ER membrane protein complex subunit 4 × 1 （Q5J8M3） 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 3 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 9Z9 (3beta,14beta,17beta,25R)-3-[4-methoxy-3-(methoxymethyl)butoxy]spirost-5-en × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 8 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.40 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8EOI Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
8EOG Structure of the human L-type voltage-gated calcium channel Cav1.2 complexed with L-leucine Deposited 2022-10-03 Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 3 PDB declaration: trimeric
Chain C 171–361（191 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 5 CA CALCIUM ION × 3 NA SODIUM ION × 1 LEU LEUCINE × 1 CLR CHOLESTEROL × 3 WNZ (2R)-3-{[(R)-(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-2-(dodecanoyloxy)propyl dodecanoate × 1 WO9 (2R)-3-{[(R)-(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-2-(dodecanoyloxy)propyl heptadecanoate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8FD7 Structure of the human L-type voltage-gated calcium channel Cav1.2 complexed with gabapentin Deposited 2022-12-02 Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 3 PDB declaration: trimeric
Chain C 1–477（477 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 8 GBN [1-(AMINOMETHYL)CYCLOHEXYL]ACETIC ACID × 1 CA CALCIUM ION × 3 NA SODIUM ION × 1 WO9 (2R)-3-{[(R)-(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-2-(dodecanoyloxy)propyl heptadecanoate × 1 YSW (2S)-3-{[(R)-(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-2-(dodecanoyloxy)propyl dodecanoate × 1 CLR CHOLESTEROL × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å

2 other PDB entries and 2 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name CACB3_RABIT
Isoform —
PDB entities 10
Chains and sequence ranges Author chain J； PDBConstruct 1–324； UniProt 38–361

ER membrane protein complex subunit 4

Homo sapiens

UniProt Q5J8M3

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Other combination Heteromer Protein × 11 其他Polymer 3 PDB declaration: undecameric（11） Consistent with protein copy count Chain D； UniProt 14–183 Not recorded ER membrane protein complex subunit 1 × 1 （Q8N766） ER membrane protein complex subunit 2 × 1 （Q15006） ER membrane protein complex subunit 3 × 1 （Q9P0I2） ER membrane protein complex subunit 5 × 1 （Q8N4V1） ER membrane protein complex subunit 6 × 1 （Q9BV81） ER membrane protein complex subunit 7 × 1 （Q9NPA0） ER membrane protein complex subunit 8 × 1 （O43402） ER membrane protein complex subunit 10 × 1 （Q5UCC4） Voltage-dependent L-type calcium channel subunit alpha-1C × 1 （Q13936） Voltage-dependent L-type calcium channel subunit beta-3 × 1 （P54286） 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 3 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 9Z9 (3beta,14beta,17beta,25R)-3-[4-methoxy-3-(methoxymethyl)butoxy]spirost-5-en × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 8 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.40 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8EOI Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
6Z3W Human ER membrane protein complex Deposited 2020-05-22 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 9 PDB declaration: nonameric
Chain D 1–183（183 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.40 Å
7ADO Cryo-EM structure of human ER membrane protein complex in lipid nanodiscs Deposited 2020-09-15 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric
Chain D 1–183（183 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 4 PCW 1,2-DIOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 6；10 mM ammonium citrate pH 6.0, 100 mM sodium chloride, 0.25 mM TCEP
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.39 Å
7ADP Cryo-EM structure of human ER membrane protein complex in GDN detergent Deposited 2020-09-15 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain D 1–183（183 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 6；10 mM ammonium citrate pH 6.0, 100 mM sodium chloride, 0.25 mM TCEP
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8J0N cryo-EM structure of human EMC Deposited 2023-04-11 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain D 1–183（183 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.47 Å
8J0O cryo-EM structure of human EMC and VDAC Deposited 2023-04-11 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 10 PDB declaration: decameric
Chain D 1–183（183 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.32 Å
8S9S Structure of the human ER membrane protein complex (EMC) in GDN Deposited 2023-03-30 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain 4 1–183（183 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 PCW 1,2-DIOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
9C7V Structure of the human BOS:human EMC complex in GDN Deposited 2024-06-11 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 12 PDB declaration: dodecameric
Chain 4 1–183（183 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.60 Å
9ZZ6 The ER membrane protein complex acts as a chaperone to promote voltage-gated calcium channel assembly Deposited 2026-01-06 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 11 PDB declaration: undecameric
Chain D 1–183（183 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；pH 7.5 adjusted with KOH ( potassium hydroxide).
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.16 Å

8 other PDB entries and 8 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name EMC4_HUMAN
Isoform —
PDB entities 11
Chains and sequence ranges Author chain D； PDBConstruct 1–170； UniProt 14–183

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 8eoi

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 8eoi
Download Download
▼

2. Structure Basics 2. Structure Basics

Entry ID entry_id8eoi
Deposition date deposition_date2022-10-03
Structure title titleStructure of a human EMC:human Cav1.2 channel complex in GDN detergent
Keywords keywordsendoplasmic reticulum membrane protein complex, voltage-gated calcium channel, holdase, biogenesis, MEMBRANE PROTEIN; MEMBRANE PROTEIN
Experimental Method methodELECTRON MICROSCOPY
▼

3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier64.63 Å
Radius of gyration Rg (electron density) rg_electron64.02 Å
Forward intensity I(0) i02156720000.00
Molecular weight molecular_weight407250.0 kDa
Excluded volume excluded_volume516870 ų
Envelope volume envelope_volume857670 ų
Hydration-shell volume shell_volume116840 ų
Envelope diameter envelope_diameter225.5 Å
Shell Rg shell_rg60.73 Å
Envelope Rg envelope_rg61.17 Å
Shape Rg shape_rg64.03 Å
Total Rg total_rg63.93 Å
Total atoms total_atoms28721
Residues n_residues3562
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
▼

4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax230.8 Å
Rg (real space) rg_real64.71 Å
Rg uncertainty (real space) rg_real_error2.53 Å
I(0) (real space) i0_real2.1570e+09
I(0) uncertainty (real space) i0_real_error4.6730e+07
Rg (reciprocal space) rg_reciprocal64.52 Å
I(0) (reciprocal space) i0_reciprocal2156000000.0000
Solution quality estimate total_estimate0.8717
Solution quality rating solution_quality GOOD a GOOD solution
P(r) peaks n_peaks1
Primary peak position r_peak_primary76.9 Å
Skewness Skewness skewness0.295
Kurtosis Kurtosis kurtosis-0.437
Angular range angular_range— – 0.1200 Å−1
Current regularization parameter α current_alpha0.0010
Highest regularization parameter α highest_alpha149400000.0000
Real-space data points n_real_points25
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.836; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.997; Smooth: 0.824
▶

5. Crystallography and Experiment 5. Crystallography & Experiment

▶

6. Entities and Polymers Entities & Polymers (14)

▼

7. Fold Classification (SCOP + CATH) 3 domains

CATH v4.4 (3 domains)

Domain ID domain_id8eoiJ01
Class class2 — Mainly Beta
Architecture architecture30 — Roll
Topology topology30 — SH3 type barrels.
Homologous superfamily homologous superfamily40 — SH3 Domains
Domain ID domain_id8eoiJ02
Class class3 — Alpha Beta
Architecture architecture40 — 3-Layer(aba) Sandwich
Topology topology50 — Rossmann fold
Homologous superfamily homologous superfamily300 — P-loop containing nucleotide triphosphate hydrolases
Domain ID domain_id8eoiK01
Class class1 — Mainly Alpha
Architecture architecture20 — Up-down Bundle
Topology topology120 — Four Helix Bundle (Hemerythrin (Met), subunit A)
Homologous superfamily homologous superfamily350 — Voltage-gated potassium channels. Chain C
▶

8. Citations (1)

▶

9. Files and Curves (10)