8iah

Structure of mammalian spectrin-actin junctional complex of membrane skeleton, State I, Global map

Method: ELECTRON MICROSCOPY Dmax: 329.8 Å Quality: GOOD

SAXS 散射曲线 SAXS Profile

SAXS profile for 8iah

P(r) 距离分布 P(r) Distribution

P(r) distribution for 8iah
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1. 结构基本信息 1. Structure Basics

条目编号 entry_id8iah
沉积日期 deposition_date2023-02-08
结构标题 titleStructure of mammalian spectrin-actin junctional complex of membrane skeleton, State I, Global map
关键词 keywordsMacrocomplex, membrane skeleton, spectrin-actin junction, MEMBRANE PROTEIN; MEMBRANE PROTEIN
实验方法 methodELECTRON MICROSCOPY

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier
回转半径 Rg (电子) rg_electron119.30
零角强度 I(0) i015839100000.00
分子量 molecular_weight1059200.0 kDa
排除体积 excluded_volume1321300 ų
包络体积 envelope_volume2276900 ų
水化壳体积 shell_volume185600 ų
包络直径 envelope_diameter425.4
壳层 Rg shell_rg75.91
包络 Rg envelope_rg118.40
形状 Rg shape_rg119.40
总 Rg total_rg118.90
总原子数 total_atoms74320
残基数 n_residues9387
球谐函数阶数 n_harmonics20
q 范围 q_range— – 0.5000 −1
数据点数 n_points101
壳层类型 shell_typedirectional
溶剂电子密度 solvent_density0.3340 e/ų
壳层衬度 contrast_shell0.0300 e/ų
CRYSOL 版本 crysol_version4.1.3

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax329.8
Rg (实空间) rg_real108.30
Rg 误差 (实空间) rg_real_error2.44
I(0) (实空间) i0_real1.5200e+10
I(0) 误差 (实空间) i0_real_error3.8570e+08
Rg (倒空间) rg_reciprocal96.30
I(0) (倒空间) i0_reciprocal14880000000.0000
解质量估计 total_estimate0.8649
解质量评级 solution_quality GOOD a GOOD solution
P(r) 峰数 n_peaks1
主峰位置 r_peak_primary76.1
偏度 Skewness skewness0.483
峰度 Kurtosis kurtosis-0.737
角度范围 angular_range— – 0.0650 −1
当前正则化参数 α current_alpha0.6453
最高正则化参数 α highest_alpha307800000.0000
实空间数据点数 n_real_points14
GNOM 版本 gnom_version4.1.3
质量判据 quality_criteria AN1: 0.006; Oscil: 0.793; Stabil: 0.972; Sysdev: 1.000; Positv: 1.000; Valcen: 0.990; Smooth: 0.013

4. 晶体学与实验 4. Crystallography & Experiment

5. 实体与聚合体信息 (11)

6. 折叠分类 (SCOP + CATH) 12 domains

CATH v4.4 (12 domains)

结构域编号 domain_id8iahA01
类 Class class3 — Alpha Beta
架构 Architecture architecture90 — Alpha-Beta Complex
拓扑 Topology topology640 — Actin; Chain A, domain 4
同源超家族 H-superfamily homologous superfamily10 — ATPase, substrate binding domain, subdomain 4
结构域编号 domain_id8iahB01
类 Class class3 — Alpha Beta
架构 Architecture architecture90 — Alpha-Beta Complex
拓扑 Topology topology640 — Actin; Chain A, domain 4
同源超家族 H-superfamily homologous superfamily10 — ATPase, substrate binding domain, subdomain 4
结构域编号 domain_id8iahC01
类 Class class3 — Alpha Beta
架构 Architecture architecture90 — Alpha-Beta Complex
拓扑 Topology topology640 — Actin; Chain A, domain 4
同源超家族 H-superfamily homologous superfamily10 — ATPase, substrate binding domain, subdomain 4
结构域编号 domain_id8iahD01
类 Class class3 — Alpha Beta
架构 Architecture architecture90 — Alpha-Beta Complex
拓扑 Topology topology640 — Actin; Chain A, domain 4
同源超家族 H-superfamily homologous superfamily10 — ATPase, substrate binding domain, subdomain 4
结构域编号 domain_id8iahE01
类 Class class3 — Alpha Beta
架构 Architecture architecture90 — Alpha-Beta Complex
拓扑 Topology topology640 — Actin; Chain A, domain 4
同源超家族 H-superfamily homologous superfamily10 — ATPase, substrate binding domain, subdomain 4
结构域编号 domain_id8iahF01
类 Class class3 — Alpha Beta
架构 Architecture architecture90 — Alpha-Beta Complex
拓扑 Topology topology640 — Actin; Chain A, domain 4
同源超家族 H-superfamily homologous superfamily10 — ATPase, substrate binding domain, subdomain 4
结构域编号 domain_id8iahH01
类 Class class3 — Alpha Beta
架构 Architecture architecture90 — Alpha-Beta Complex
拓扑 Topology topology640 — Actin; Chain A, domain 4
同源超家族 H-superfamily homologous superfamily10 — ATPase, substrate binding domain, subdomain 4
结构域编号 domain_id8iahI01
类 Class class3 — Alpha Beta
架构 Architecture architecture90 — Alpha-Beta Complex
拓扑 Topology topology640 — Actin; Chain A, domain 4
同源超家族 H-superfamily homologous superfamily10 — ATPase, substrate binding domain, subdomain 4
结构域编号 domain_id8iahJ01
类 Class class3 — Alpha Beta
架构 Architecture architecture90 — Alpha-Beta Complex
拓扑 Topology topology640 — Actin; Chain A, domain 4
同源超家族 H-superfamily homologous superfamily10 — ATPase, substrate binding domain, subdomain 4
结构域编号 domain_id8iahK01
类 Class class3 — Alpha Beta
架构 Architecture architecture90 — Alpha-Beta Complex
拓扑 Topology topology640 — Actin; Chain A, domain 4
同源超家族 H-superfamily homologous superfamily10 — ATPase, substrate binding domain, subdomain 4
结构域编号 domain_id8iahL01
类 Class class3 — Alpha Beta
架构 Architecture architecture90 — Alpha-Beta Complex
拓扑 Topology topology640 — Actin; Chain A, domain 4
同源超家族 H-superfamily homologous superfamily10 — ATPase, substrate binding domain, subdomain 4
结构域编号 domain_id8iahY01
类 Class class3 — Alpha Beta
架构 Architecture architecture80 — Alpha-Beta Horseshoe
拓扑 Topology topology10 — Leucine-rich repeat, LRR (right-handed beta-alpha superhelix)
同源超家族 H-superfamily homologous superfamily10 — Ribonuclease Inhibitor

7. 引用文献 (1)

8. 文件与曲线 (10)