8iai

Structure of mammalian spectrin-actin junctional complex of membrane skeleton, State II, Global map

Method: ELECTRON MICROSCOPY D_max: 310.4 Å Quality: GOOD

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

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1. 结构基本信息 1. Structure Basics

条目编号 entry_id	8iai
沉积日期 deposition_date	2023-02-08
结构标题 title	Structure of mammalian spectrin-actin junctional complex of membrane skeleton, State II, Global map
关键词 keywords	Macrocomplex, membrane skeleton, spectrin-actin junction, MEMBRANE PROTEIN; MEMBRANE PROTEIN
实验方法 method	ELECTRON MICROSCOPY

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2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	— Å
回转半径 Rg (电子) rg_electron	116.50 Å
零角强度 I(0) i0	10369100000.00
分子量 molecular_weight	859650.0 kDa
排除体积 excluded_volume	1074100 Å³
包络体积 envelope_volume	1807900 Å³
水化壳体积 shell_volume	156200 Å³
包络直径 envelope_diameter	426.1 Å
壳层 Rg shell_rg	70.71 Å
包络 Rg envelope_rg	117.50 Å
形状 Rg shape_rg	116.50 Å
总 Rg total_rg	115.90 Å
总原子数 total_atoms	60300
残基数 n_residues	7612
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

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3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	310.4 Å
Rg (实空间) rg_real	103.30 Å
Rg 误差 (实空间) rg_real_error	2.31 Å
I(0) (实空间) i0_real	9.9190e+09
I(0) 误差 (实空间) i0_real_error	2.4010e+08
Rg (倒空间) rg_reciprocal	93.33 Å
I(0) (倒空间) i0_reciprocal	9774000000.0000
解质量估计 total_estimate	0.8782
解质量评级 solution_quality	GOOD a GOOD solution
P(r) 峰数 n_peaks	1
主峰位置 r_peak_primary	71.6 Å
偏度 Skewness skewness	0.460
峰度 Kurtosis kurtosis	-0.771
角度范围 angular_range	— – 0.0650 Å⁻¹
当前正则化参数 α current_alpha	0.3741
最高正则化参数 α highest_alpha	127900000.0000
实空间数据点数 n_real_points	14
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.012; Oscil: 0.827; Stabil: 0.990; Sysdev: 1.000; Positv: 1.000; Valcen: 0.970; Smooth: 0.030

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4. 晶体学与实验 4. Crystallography & Experiment

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5. 实体与聚合体信息 (10)

Entity 1 polymer

描述 description	Adducin 1
分子量 formula_weight	81307.2 kDa
来源方法 src_method	nat
拷贝数 entity_count	3

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	1,2,9
原子数 atom_count	60300

MNGDTRAAVVTSPPPTTAPHKERYFDRVDENNPEYLRERNMAPDLRQDFNMMEQKKRVSMILQSPAFCEELESMIQEQFKKGKNPTGLLALQQIADFMTTNVPNVYPAAPQGGMAALNMSLGMVTPVNDLRGSDSIAYDKGEKLLRCKLAALYRLADLFGWSHLIYNHITTRVSSEQEHFLIVPFGLLYSEVTASSLVKINLQGDVVDRGSTNLGVNQAGFTLHSAVYAARPDVKCVVHIHTPAGAAVSAMKCGLLPISPEALSLGEVAYHDYHGILVDEEEKVLIQKNLGPKSKVLILRNHGLVSVGESVEEAFYYIHNLVVACEIQVRTLASAGGPDNLVLLDPGKYKAKSRPPTSPAGEGSGSPPAWQIGEQEFEALMRMLDNLGYRTGYPYRYPALREKSKKYSDVEVPASVTGYSVASDGDSGTGSPLRHSFQKQQREKTRWLNSGRGDDASEEGQAGSSPKSKTKWTKEDGHRASASAVPNLFVPLNTNPKEVQEMRNKIREQNLQDIKTAGPQSQVLCGVVMDRSLVQGELVTASKAIIEKEYQPHVVVSTTGPNPFNTLTDRELEEYRREVERKQKGPEETLDERSDQKEDSPPEPPAAPHTPPSTPVKLEEDLPQEPLSGDDSEAATFRPTLPDLPPDEPSEVLGFPTLEEEEEEEEVGALCEAGRPPSPARPAAEASPEPAAAQAAEEPASPAAEEGAAADPGSDGSPGKSPSKKKKKFRTPSFLKKSKKRSDS

Entity 2 polymer

描述 description	Beta-adducin
分子量 formula_weight	80705.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	2

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	3,4

MSEETVPEAASPPPAQGRQYFDRFSEEDPEYLRLRNRAADLRQDFNLMEQKKRVTMILQSPSFREELEGLIQEQMKKGNNSSNIWALRQIADFMASTSHAVFPTSSMNFSMMTPINDLHTADSLNLAKGERLMRCKISSVYRLLDLYGWAQLSDTYVTLRVSKEQDHFLISPKGVSCSEVTASSLIKVNILGEVVEKGSSCFPVDTTGFCLHSAIYAARPDVRCIIHLHTPATAAVSAMKWGLLPVSHNALLVGDMAYFDFNGEMEQEADRINLQKCLGPTCKILVLRNHGVVALGDTVEEAFYKVFHLQAACEIQVSALSSAGGVENLILLEQEKHRPHEVGSVQWAGSTFGPMQKSRLGEHEFEALMRMLDNLGYRTGYPYRYPLVQEKTKHKSEVEIPATVTAFVFEEDGAPVPALRQHAQKQQKEKTRWLNTPNTYLRVNVADEVQRSMGSPRPKTTWMKADEVEKSSSGMPIRIENPNQFVPLYTDPQEVLDMRNKIREQNRQDVKSAGPQSQLLASVIAEKSRSPSTESQLMSQGQADTKDESEETVPNPFSQLTDQELEEYKKEVERKKLELDGEKEPAVEEPGSPGKSAPASPAQSPAKSETKSPVGSPSKSVDEEAQKTEPSKPTTEPETTQPEGVVVNGREDEPTAEEILSKGLSQMTTHADTDVDTSKDKTESVTSGPMSPEGSPSKSPSKKKKKFRTPSFLKKSKKKEKVES

Entity 3 polymer

描述 description	Dematin actin binding protein
分子量 formula_weight	45569.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	3

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	5,6,7

MERLQKQPLTSPGSVSSSRGSSVPGSPSSIVAKMDNQVLGYKDLAAIPKDKAILDIERPDLMIYEPHFTYSLLEHVELPRSRERSLSPKSTSPPPSPEVWAESRSPGTFPQASAPRTTGTPRTSLPHFHHPETTRPDSNIYKKPPIYKQREPTGGSPQSKHLIEDLIIESSKFPAAQPPDPNQPAKIETDYWPCPPSLAVVETEWRKRKASRRGAEEEEEEEDDDSGEEMKALRERQREELSKVTSNLGKMILKEEMEKSLPIRRKTRSLPDRTPFHTSLQAGTSKSSSLPAYGRTTLSRLQSTDFSPSGSETESPGLQNGEGQRGRMDRGTSLPCVLEQKIYPYEMLVVTNKGRTKLPPGVDRMRLERHLSAEDFSRVFSMSPEEFGKLALWKRNELKKKASLF

Entity 4 polymer

描述 description	Actin, cytoplasmic 1
分子量 formula_weight	41782.7 kDa
来源方法 src_method	nat
拷贝数 entity_count	11

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	A,B,C,D,E,F,G,H,I,J,K

MDDDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF

Entity 5 polymer

描述 description	Spectrin beta chain
分子量 formula_weight	248510.7 kDa
来源方法 src_method	nat
拷贝数 entity_count	8

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	M,N,O,P,Q,R,S,T

MTSATEFENVGNQPPYSRINARWDAPDDELDNDNSSARLFERSRIKALADEREVVQKKTFTKWVNSHLARVSCRITDLYKDLRDGRMLIKLLEVLSGEMLPKPTKGKMRIHCLENVDKALQFLKEQRVHLENMGSHDIVDGNHRLVLGLIWTIILRFQIQDIVVQTQEGRETRSAKDALLLWCQMKTAGYPNVNVTNFTSSWKDGLAFNALIHKHRPDLIDFDKLKDSNARHNLEHAFDVAERQLGIIQLLDPEDVFTENPDEKSIITYVVAFYHYFSKMKVLAVEGKRVGKVIDHAIETEKMIEKYSGLASDLLTWIEQTITVLNSRKFANSLAGVQQQLQAFSTYRTVEKPPKFQEKGNLEVLLFTIQSRMRANNQKVYTPHDGKLVSDINRAWESLEEAEYRRELALRSELIRQEKLEQLARRFDRKAAMRETWLNENQRLVAQDNFGYDLAAVEAAKKKHEAIETDTAAYEERVRALEDLARELELENYHDQKRITARKDNILRLWNYLQELLQSRRQRLETTLALQQLFQDMLHSIDWMDEIKAHLLSAEFGKHLLEAEDLLQKHKLMEADIAIQGDKVKAITAATLQFTEETGYQPCDPQVIRDRVSHLEQCFAELSNTAAGRKAQLEQSKRLWKLFWEMDEAKSWIKEKEQIYSSLGYGKDLTSVLILQRKHKAFEDELRRLDPHLDQIFQEAEDMVALKQFGYPKNEAWVKEVSAQWDQLKEVPAQWNQLKELAASRKKNLQDTENFFQFQGDVDDLKAWLQDAHKLLSGEDVGQDEGATRALGKKHKDFLEELEESRGVMEHLEQQAQDFPERFRDSPDVTNRLQVLRDLYQQVVAQADLRRQRLQDALDLYTVFGETDACELWMGEKEKWLAQMDIPDTLEDLEVVQHRFDILDQEMKTLIAQIDGVNVAANSLVESNHPRSTEVKQYQDHLNTRWREFQTMVLARREAVDSALRVHNYCVDCEETSKWIIDKTKVVESTKDLGQDLAGVMAIQRKLSGLERDVAAIQVRVGALEQESHRLMESHREQEKDIGERQAYVEELWQGLQQALKGQEALLGKSSQLQAFLQDLDAFEAWLSTAQKEVASKDMPESLPEAEQLLQQHAALKDDIDRHQENYQHVKASGEKVIHGQTDPEYLLLGQRLEGLDKGWDALCRMWESRGHTLAQCLGFQEFQKDAKQAEAILSNQEYTLAHLEPPDSLEAAEAGIRKFQDFFTTMENNRDKVLSPVDSGNKLVAEGNLYSDKIKEKVQQIEDRHKRNNEKAQEASVLLQDNLALQNFLQNCQELTLWINDKLLTSQDVSYDDARNLHNKWLKHTAFEAELASQQGWLENIDAEGKQLMEEKPQFEALVSQRLEALHRLWDELQATTKEIGQRLSAARSSDLRSQTHADLNKWIRAMEDQLRSDDLGKDLTSVNRMLAKLKRVEDQVNVRKEELGELFAHMPSPGEEAEDEDLSIEKRFLDLLEPLGRRKKQLESSKAKLQISRDLEDETLWVEERLPLAQSTDYGANLQTVQLFMKKNQTLQNEILGHAPRVEDVLYRGHQLVEAAEIDCQDIEERLGHLQNSWDTLQEAAAGRLQRLWDASEAQQYYLDAGEAEAWISEQELYVISDEMPQDEEGAIVMLKRHLRQQRMVEEYERNIKQLAGRAQSLLAAGHPEGEQIIRLQGQVDKHYAGLKDMAEDRKRKLENKYRQFQMEREADDLEQWILEKDLVASSPEMGQDFDHVTLLRDKFRDFARETGAIGQERVDNMNALIEHLIDAGHEEAASIAERKDGLNEMWADVLELIDTRMQLLAASYDRHRYFYTGNEILGLIDEKHRELPEDVGLDASTAESFHRVHTAFERELHQLGVQVQQFQDVATRLQAAYAGEEADSIQNKEQEVSAAWQALLDACAGRRTQLVDTADKFRFFSMVRDLLSWMETIIRQIETQERPRDVSSVELLMKYHQGIWAEMDTRSKNFSACLELGESLLQRQHQASDEIREKLQQVVSKKKEIDEKWKARSDRLSMLLEVCQFSRDASVAEAWLIAQEPYLSSRDFGHTVDSVEKLIKRHEAFEKSTASWEPRFAALEKPTTLELKERQTPERPKEDAGPQEEEGETAGEAPRGPHRAATERTSPVSSLSHLSSSWESLLPEPAHPY

Entity 6 polymer

描述 description	Tropomyosin-1.9
分子量 formula_weight	28791.2 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	U

MAGSSSLEAVRRKIRSLQEQADAAEERAGTLQRELDYERKLRETAEADVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIESRAQKDEEKMEIQEIQLKEAKHIAEDADRKYEEVARKLVIIESDLERAEERAELSEGQVRQLEEQLRIMDQTLKALMAAEDKYSQKEDKYEEEIKVLSDKLKEAETRAEFAERSVTKLEKSIDDLEEKVAHAKEENLSMHQMLDQTLLELNNM

Entity 7 polymer

描述 description	Tropomyosin 3
分子量 formula_weight	29080.7 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	V

MAGITTIEAVKRKIQVLQQQADDAEERAERLQREVEGERRAREQAEAEVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIENRALKDEEKMELQEIQLKEAKHIAEEADRKYEEVARKLVIIEGDLERTEERAELAESRCREMDEQIRLMDQNLKCLSAAEEKYSQKEDKYEEEIKILTDKLKEAETRAEFAERSVAKLEKTIDDLEDKLKCTKEEHLCTQRMLDQTLLDLNEM

Entity 8 polymer

描述 description	Tropomodulin-1
分子量 formula_weight	40523.1 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	Y

MSYRRELEKYRDLDEDEILGALTEEELRTLENELDELDPDNALLPAGLRQKDQTTKAPTGPFKREELLDHLEKQAKEFKDREDLVPYTGEKRGKVWVPKQKPMDPVLETVTLEPELEEALANASDAELCDIAAILGMHTLMSNQQYYQALGSSSIVNKEGLNSVIKPTQYKPVPDEEPNATDVEETLERIKNNDPKLEEVNLNNIRNIPIPTLKAYAEALKENSYVKKFSIVGTRSNDPVAFALAEMLKVNKVLKTLNVESNFISGAGILRLVEALPYNTSLVELKIDNQSQPLGNKVEMEIVSMLEKNATLLKFGYHFTQQGPRLRASNAMMNNNDLVRKRRLADLTGPIIPKCRSGI

Entity 9 polymer

描述 description	SH3 domain-binding glutamic acid-rich-like protein
分子量 formula_weight	12285.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	Z

MVIRVFIASSSGFVAIKKKQQDVVRFLEANKIEFEEVDITMSEEQRQWMYKNIPPEKKPAQGNPLPPQIFNGDRYCGDYDSFFESKESNTVFSFLGLKSQLASKAEP

Entity 10 non-polymer

描述 description	ADENOSINE-5'-DIPHOSPHATE
分子量 formula_weight	427.2 kDa
来源方法 src_method	syn
拷贝数 entity_count	11

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6. 折叠分类 (SCOP + CATH) 11 domains

CATH v4.4 (11 domains)

结构域编号 domain_id	8iaiA01
类 Class class	3 — Alpha Beta
架构 Architecture architecture	90 — Alpha-Beta Complex
拓扑 Topology topology	640 — Actin; Chain A, domain 4
同源超家族 H-superfamily homologous superfamily	10 — ATPase, substrate binding domain, subdomain 4

结构域编号 domain_id	8iaiB01
类 Class class	3 — Alpha Beta
架构 Architecture architecture	90 — Alpha-Beta Complex
拓扑 Topology topology	640 — Actin; Chain A, domain 4
同源超家族 H-superfamily homologous superfamily	10 — ATPase, substrate binding domain, subdomain 4

结构域编号 domain_id	8iaiC01
类 Class class	3 — Alpha Beta
架构 Architecture architecture	90 — Alpha-Beta Complex
拓扑 Topology topology	640 — Actin; Chain A, domain 4
同源超家族 H-superfamily homologous superfamily	10 — ATPase, substrate binding domain, subdomain 4

结构域编号 domain_id	8iaiD01
类 Class class	3 — Alpha Beta
架构 Architecture architecture	90 — Alpha-Beta Complex
拓扑 Topology topology	640 — Actin; Chain A, domain 4
同源超家族 H-superfamily homologous superfamily	10 — ATPase, substrate binding domain, subdomain 4

结构域编号 domain_id	8iaiE01
类 Class class	3 — Alpha Beta
架构 Architecture architecture	90 — Alpha-Beta Complex
拓扑 Topology topology	640 — Actin; Chain A, domain 4
同源超家族 H-superfamily homologous superfamily	10 — ATPase, substrate binding domain, subdomain 4

结构域编号 domain_id	8iaiF01
类 Class class	3 — Alpha Beta
架构 Architecture architecture	90 — Alpha-Beta Complex
拓扑 Topology topology	640 — Actin; Chain A, domain 4
同源超家族 H-superfamily homologous superfamily	10 — ATPase, substrate binding domain, subdomain 4

结构域编号 domain_id	8iaiG01
类 Class class	3 — Alpha Beta
架构 Architecture architecture	90 — Alpha-Beta Complex
拓扑 Topology topology	640 — Actin; Chain A, domain 4
同源超家族 H-superfamily homologous superfamily	10 — ATPase, substrate binding domain, subdomain 4

结构域编号 domain_id	8iaiH01
类 Class class	3 — Alpha Beta
架构 Architecture architecture	90 — Alpha-Beta Complex
拓扑 Topology topology	640 — Actin; Chain A, domain 4
同源超家族 H-superfamily homologous superfamily	10 — ATPase, substrate binding domain, subdomain 4

结构域编号 domain_id	8iaiI01
类 Class class	3 — Alpha Beta
架构 Architecture architecture	90 — Alpha-Beta Complex
拓扑 Topology topology	640 — Actin; Chain A, domain 4
同源超家族 H-superfamily homologous superfamily	10 — ATPase, substrate binding domain, subdomain 4

结构域编号 domain_id	8iaiJ01
类 Class class	3 — Alpha Beta
架构 Architecture architecture	90 — Alpha-Beta Complex
拓扑 Topology topology	640 — Actin; Chain A, domain 4
同源超家族 H-superfamily homologous superfamily	10 — ATPase, substrate binding domain, subdomain 4

结构域编号 domain_id	8iaiK01
类 Class class	3 — Alpha Beta
架构 Architecture architecture	90 — Alpha-Beta Complex
拓扑 Topology topology	640 — Actin; Chain A, domain 4
同源超家族 H-superfamily homologous superfamily	10 — ATPase, substrate binding domain, subdomain 4

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7. 引用文献 (1)

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晶胞边长 a length_a	1.000 Å
晶胞边长 b length_b	1.000 Å
晶胞边长 c length_c	1.000 Å
晶胞夹角 α angle_alpha	90.00 °
晶胞夹角 β angle_beta	90.00 °
晶胞夹角 γ angle_gamma	90.00 °
晶胞分子数 Z z_pdb	—

空间群 space_group	P 1
空间群编号 int_tables_number	1
晶系 crystal_system	—

分辨率 resolution	3.50 Å
分辨率测定方法 resolution_method	—
颗粒数 num_particles	68000

fit	/app/data/saxs_computation/ia/8iai/8iai.fit
pddf	/app/data/pr_computation/ia/8iai/8iai.pr.out
profile	/app/data/saxs_computation/ia/8iai/8iai.dat

Structure of mammalian spectrin-actin junctional complex of membrane skeleton, State II, Global map

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

1. 结构基本信息 1. Structure Basics

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

4. 晶体学与实验 4. Crystallography & Experiment

晶胞参数 Unit Cell

对称性 Symmetry

冷冻电镜 EM 3D

5. 实体与聚合体信息 (10)

Entity 1 polymer

聚合体 Polymer

Entity 2 polymer

聚合体 Polymer

Entity 3 polymer

聚合体 Polymer

Entity 4 polymer

聚合体 Polymer

Entity 5 polymer

聚合体 Polymer

Entity 6 polymer

聚合体 Polymer

Entity 7 polymer

聚合体 Polymer

Entity 8 polymer

聚合体 Polymer

Entity 9 polymer

聚合体 Polymer

Entity 10 non-polymer

6. 折叠分类 (SCOP + CATH) 11 domains

CATH v4.4 (11 domains)

7. 引用文献 (1)

8. 文件与曲线 (10)

Files (7)

Curves (3)