8wki

Cryo-EM structure of the distal rod-hook within the flagellar motor-hook complex in the CW state.

Method: ELECTRON MICROSCOPY D_max: 249.3 Å Quality: EXCELLENT

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

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1. 结构基本信息 1. Structure Basics

条目编号 entry_id	8wki
沉积日期 deposition_date	2023-09-27
结构标题 title	Cryo-EM structure of the distal rod-hook within the flagellar motor-hook complex in the CW state.
关键词 keywords	Flagellum, Flagellar motor, Rod, Hook, MOTOR PROTEIN; MOTOR PROTEIN
实验方法 method	ELECTRON MICROSCOPY

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2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	90.66 Å
回转半径 Rg (电子) rg_electron	91.08 Å
零角强度 I(0) i0	52371800000.00
分子量 molecular_weight	1870700.0 kDa
排除体积 excluded_volume	2307400 Å³
包络体积 envelope_volume	3537600 Å³
水化壳体积 shell_volume	312850 Å³
包络直径 envelope_diameter	342.8 Å
壳层 Rg shell_rg	92.40 Å
包络 Rg envelope_rg	92.88 Å
形状 Rg shape_rg	91.07 Å
总 Rg total_rg	91.12 Å
总原子数 total_atoms	131528
残基数 n_residues	17770
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

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3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	249.3 Å
Rg (实空间) rg_real	86.54 Å
Rg 误差 (实空间) rg_real_error	0.81 Å
I(0) (实空间) i0_real	5.0220e+10
I(0) 误差 (实空间) i0_real_error	1.0620e+09
Rg (倒空间) rg_reciprocal	89.11 Å
I(0) (倒空间) i0_reciprocal	52120000000.0000
解质量估计 total_estimate	0.9151
解质量评级 solution_quality	EXCELLENT a EXCELLENT solution
P(r) 峰数 n_peaks	2
主峰位置 r_peak_primary	102.7 Å
偏度 Skewness skewness	0.350
峰度 Kurtosis kurtosis	-0.431
角度范围 angular_range	— – 0.0850 Å⁻¹
当前正则化参数 α current_alpha	0.5125
最高正则化参数 α highest_alpha	10170000000.0000
实空间数据点数 n_real_points	18
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.000; Oscil: 0.982; Stabil: 0.987; Sysdev: 1.000; Positv: 1.000; Valcen: 0.987; Smooth: 0.004

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4. 晶体学与实验 4. Crystallography & Experiment

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5. 实体与聚合体信息 (2)

Entity 1 polymer

描述 description	Flagellar hook protein FlgE
分子量 formula_weight	42233.2 kDa
来源方法 src_method	nat
拷贝数 entity_count	29

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	ZF,ZG,ZH,ZI,ZJ,ZK,ZL,ZM,ZN,ZO,ZP,ZQ,ZR,ZS,ZT,ZU,ZV,ZW,ZX,ZY,ZZ,Za,Zb,Zc,Zd,Ze,Zf,Zg,Zh
原子数 atom_count	131528

MSFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFAGSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKLDENRNLVNMQGMQLTGYPATGTPPTIQQGANPAPITIPNTLMAAKSTTTASMQINLNSTDPVPSKTPFSVSDADSYNKKGTVTVYDSQGNAHDMNVYFVKTKDNEWAVYTHDSSDPAATAPTTASTTLKFNENGILESGGTVNITTGTINGATAATFSLSFLNSMQQNTGANNIVATNQNGYKPGDLVSYQINNDGTVVGNYSNEQEQVLGQIVLANFANNEGLASQGDNVWAATQASGVALLGTAGSGNFGKLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQILNTLVNLR

Entity 2 polymer

描述 description	Flagellar basal-body rod protein FlgG
分子量 formula_weight	27784.8 kDa
来源方法 src_method	nat
拷贝数 entity_count	24

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	2,3,4,5,6,7,8,9,ZA,ZB,ZC,ZD,ZE,0,1,r,s,t,u,v,w,x,y,z

MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDLLYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGFFQVMLPDGTSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVTQQGQAAPVQVGQLNLTTFMNDTGLESIGENLYIETQSSGAPNESTPGLNGAGLLYQGYVETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL

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7. 引用文献 (1)

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晶胞边长 a length_a	1.000 Å
晶胞边长 b length_b	1.000 Å
晶胞边长 c length_c	1.000 Å
晶胞夹角 α angle_alpha	90.00 °
晶胞夹角 β angle_beta	90.00 °
晶胞夹角 γ angle_gamma	90.00 °
晶胞分子数 Z z_pdb	—

空间群 space_group	P 1
空间群编号 int_tables_number	1
晶系 crystal_system	—

分辨率 resolution	3.30 Å
分辨率测定方法 resolution_method	—
颗粒数 num_particles	24190

fit	/app/data/saxs_computation/wk/8wki/8wki.fit
pddf	/app/data/pr_computation/wk/8wki/8wki.pr.out
profile	/app/data/saxs_computation/wk/8wki/8wki.dat

Cryo-EM structure of the distal rod-hook within the flagellar motor-hook complex in the CW state.

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

1. 结构基本信息 1. Structure Basics

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

4. 晶体学与实验 4. Crystallography & Experiment

晶胞参数 Unit Cell

对称性 Symmetry

冷冻电镜 EM 3D

5. 实体与聚合体信息 (2)

Entity 1 polymer

聚合体 Polymer

Entity 2 polymer

聚合体 Polymer

7. 引用文献 (1)

8. 文件与曲线 (10)

Files (7)

Curves (3)