7e82

Cryo-EM structure of the flagellar rod with partial hook from Salmonella

Method: ELECTRON MICROSCOPY

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1. Protein Identity and Related Structures Protein Identity & Related Structures

Flagellar basal-body rod protein FlgG

OrganismNot specified

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 67	Flagellar basal-body rod protein FlgF × 5 （P16323） Flagellar MS ring L2 × 5 Flagellar MS ring L1 × 5 （P15928） Flagellar basal-body rod protein FlgC × 6 （P0A1I7） Flagellar basal body rod protein FlgB × 5 （P16437） Flagellar hook-basal body complex protein FliE × 6 （P26462） Flagellar hook protein FlgE × 11 （P0A1J1）	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	FLGG_SALTY
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 1–260； UniProt 1–260 Author chain B； PDBConstruct 1–260； UniProt 1–260 Author chain C； PDBConstruct 1–260； UniProt 1–260 Author chain D； PDBConstruct 1–260； UniProt 1–260 Author chain E； PDBConstruct 1–260； UniProt 1–260 Author chain F； PDBConstruct 1–260； UniProt 1–260 Author chain G； PDBConstruct 1–260； UniProt 1–260 Author chain H； PDBConstruct 1–260； UniProt 1–260 Author chain I； PDBConstruct 1–260； UniProt 1–260 Author chain J； PDBConstruct 1–260； UniProt 1–260 Author chain K； PDBConstruct 1–260； UniProt 1–260 Author chain L； PDBConstruct 1–260； UniProt 1–260 Author chain M； PDBConstruct 1–260； UniProt 1–260 Author chain N； PDBConstruct 1–260； UniProt 1–260 Author chain O； PDBConstruct 1–260； UniProt 1–260 Author chain P； PDBConstruct 1–260； UniProt 1–260 Author chain Q； PDBConstruct 1–260； UniProt 1–260 Author chain R； PDBConstruct 1–260； UniProt 1–260 Author chain S； PDBConstruct 1–260； UniProt 1–260 Author chain T； PDBConstruct 1–260； UniProt 1–260 Author chain U； PDBConstruct 1–260； UniProt 1–260 Author chain V； PDBConstruct 1–260； UniProt 1–260 Author chain W； PDBConstruct 1–260； UniProt 1–260 Author chain X； PDBConstruct 1–260； UniProt 1–260

Flagellar basal-body rod protein FlgF

OrganismNot specified

UniProt P16323

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 67	Flagellar basal-body rod protein FlgG × 24 （P0A1J3） Flagellar MS ring L2 × 5 Flagellar MS ring L1 × 5 （P15928） Flagellar basal-body rod protein FlgC × 6 （P0A1I7） Flagellar basal body rod protein FlgB × 5 （P16437） Flagellar hook-basal body complex protein FliE × 6 （P26462） Flagellar hook protein FlgE × 11 （P0A1J1）	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	FLGF_SALTY
Isoform	—
PDB entities	2
Chains and sequence ranges	Author chain a； PDBConstruct 1–251； UniProt 1–251 Author chain b； PDBConstruct 1–251； UniProt 1–251 Author chain c； PDBConstruct 1–251； UniProt 1–251 Author chain d； PDBConstruct 1–251； UniProt 1–251 Author chain e； PDBConstruct 1–251； UniProt 1–251

Flagellar MS ring L1

OrganismNot specified

UniProt P15928

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 67	Flagellar basal-body rod protein FlgG × 24 （P0A1J3） Flagellar basal-body rod protein FlgF × 5 （P16323） Flagellar MS ring L2 × 5 Flagellar basal-body rod protein FlgC × 6 （P0A1I7） Flagellar basal body rod protein FlgB × 5 （P16437） Flagellar hook-basal body complex protein FliE × 6 （P26462） Flagellar hook protein FlgE × 11 （P0A1J1）	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	FLIF_SALTY
Isoform	—
PDB entities	4
Chains and sequence ranges	Author chain 5； PDBConstruct 1–21； UniProt 311–331 Author chain 6； PDBConstruct 1–21； UniProt 311–331 Author chain 7； PDBConstruct 1–21； UniProt 311–331 Author chain 8； PDBConstruct 1–21； UniProt 311–331 Author chain 9； PDBConstruct 1–21； UniProt 311–331

Flagellar basal-body rod protein FlgC

OrganismNot specified

UniProt P0A1I7

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 67	Flagellar basal-body rod protein FlgG × 24 （P0A1J3） Flagellar basal-body rod protein FlgF × 5 （P16323） Flagellar MS ring L2 × 5 Flagellar MS ring L1 × 5 （P15928） Flagellar basal body rod protein FlgB × 5 （P16437） Flagellar hook-basal body complex protein FliE × 6 （P26462） Flagellar hook protein FlgE × 11 （P0A1J1）	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	FLGC_SALTY
Isoform	—
PDB entities	5
Chains and sequence ranges	Author chain f； PDBConstruct 1–134； UniProt 1–134 Author chain g； PDBConstruct 1–134； UniProt 1–134 Author chain h； PDBConstruct 1–134； UniProt 1–134 Author chain i； PDBConstruct 1–134； UniProt 1–134 Author chain j； PDBConstruct 1–134； UniProt 1–134 Author chain p； PDBConstruct 1–134； UniProt 1–134

Flagellar basal body rod protein FlgB

OrganismNot specified

UniProt P16437

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 67	Flagellar basal-body rod protein FlgG × 24 （P0A1J3） Flagellar basal-body rod protein FlgF × 5 （P16323） Flagellar MS ring L2 × 5 Flagellar MS ring L1 × 5 （P15928） Flagellar basal-body rod protein FlgC × 6 （P0A1I7） Flagellar hook-basal body complex protein FliE × 6 （P26462） Flagellar hook protein FlgE × 11 （P0A1J1）	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	FLGB_SALTY
Isoform	—
PDB entities	6
Chains and sequence ranges	Author chain k； PDBConstruct 1–138； UniProt 1–138 Author chain l； PDBConstruct 1–138； UniProt 1–138 Author chain m； PDBConstruct 1–138； UniProt 1–138 Author chain n； PDBConstruct 1–138； UniProt 1–138 Author chain o； PDBConstruct 1–138； UniProt 1–138

Flagellar hook-basal body complex protein FliE

OrganismNot specified

UniProt P26462

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 67	Flagellar basal-body rod protein FlgG × 24 （P0A1J3） Flagellar basal-body rod protein FlgF × 5 （P16323） Flagellar MS ring L2 × 5 Flagellar MS ring L1 × 5 （P15928） Flagellar basal-body rod protein FlgC × 6 （P0A1I7） Flagellar basal body rod protein FlgB × 5 （P16437） Flagellar hook protein FlgE × 11 （P0A1J1）	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	FLIE_SALTY
Isoform	—
PDB entities	7
Chains and sequence ranges	Author chain q； PDBConstruct 1–104； UniProt 1–104 Author chain r； PDBConstruct 1–104； UniProt 1–104 Author chain s； PDBConstruct 1–104； UniProt 1–104 Author chain t； PDBConstruct 1–104； UniProt 1–104 Author chain u； PDBConstruct 1–104； UniProt 1–104 Author chain v； PDBConstruct 1–104； UniProt 1–104

Flagellar hook protein FlgE

OrganismNot specified

UniProt P0A1J1

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 67	Flagellar basal-body rod protein FlgG × 24 （P0A1J3） Flagellar basal-body rod protein FlgF × 5 （P16323） Flagellar MS ring L2 × 5 Flagellar MS ring L1 × 5 （P15928） Flagellar basal-body rod protein FlgC × 6 （P0A1I7） Flagellar basal body rod protein FlgB × 5 （P16437） Flagellar hook-basal body complex protein FliE × 6 （P26462）	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	FLGE_SALTY
Isoform	—
PDB entities	8
Chains and sequence ranges	Author chain DA； PDBConstruct 1–403； UniProt 1–403 Author chain DB； PDBConstruct 1–403； UniProt 1–403 Author chain DC； PDBConstruct 1–403； UniProt 1–403 Author chain DD； PDBConstruct 1–403； UniProt 1–403 Author chain DE； PDBConstruct 1–403； UniProt 1–403 Author chain DF； PDBConstruct 1–403； UniProt 1–403 Author chain DG； PDBConstruct 1–403； UniProt 1–403 Author chain DH； PDBConstruct 1–403； UniProt 1–403 Author chain DI； PDBConstruct 1–403； UniProt 1–403 Author chain DJ； PDBConstruct 1–403； UniProt 1–403 Author chain DK； PDBConstruct 1–403； UniProt 1–403

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	7e82
Deposition date deposition_date	2021-02-28
Structure title title	Cryo-EM structure of the flagellar rod with partial hook from Salmonella
Keywords keywords	Flagella, Rod, MOTOR PROTEIN; MOTOR PROTEIN
Experimental Method method	ELECTRON MICROSCOPY

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

7e82__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

7e82__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

7e82__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	88.75 Å
Rg (electron density)	89.80 Å
Total Rg	89.88 Å
Atom count	101606
Residues	13700
Excluded volume	1788100 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`7e82__assembly_1__model_1`	67-meric （67）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (8)

Entity 1 Entity 1 polymer

Description description	Flagellar basal-body rod protein FlgG
Molecular weight formula_weight	27784.8 kDa
Source method src_method	nat
Copy count entity_count	24

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A,B,C,D,E,F,G,H,I,J,K,L,M,N,O,P,Q,R,S,T,U,V,W,X
Atom count atom_count	101606

MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDLLYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGFFQVMLPDGTSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVTQQGQAAPVQVGQLNLTTFMNDTGLESIGENLYIETQSSGAPNESTPGLNGAGLLYQGYVETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL

Entity 2 Entity 2 polymer

Description description	Flagellar basal-body rod protein FlgF
Molecular weight formula_weight	26121.2 kDa
Source method src_method	nat
Copy count entity_count	5

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	a,b,c,d,e

MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAVPVDGLSLATRTLVTASTPGADMTPGQLDYTSRPLDVALQQDGWLVVQAADGAEGYTRNGNIQVGPTGQLTIQGHPVIGEGGPITVPEGSEITIAADGTISALNPGDPPNTVAPVGRLKLVKAEGNEVQRSDDGLFRLTAEAQAERGAVLAADPSIRIMSGVLEGSNVKPVEAMTDMIANARRFEMQMKVITSVDENEGRANQLLSMS

Entity 3 Entity 3 polymer

Description description	Flagellar MS ring L2
Molecular weight formula_weight	1294.6 kDa
Source method src_method	nat
Copy count entity_count	5

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	0,1,2,3,4

XXXXXXXXXXXXXXX

Entity 4 Entity 4 polymer

Description description	Flagellar MS ring L1
Molecular weight formula_weight	1998.2 kDa
Source method src_method	nat
Copy count entity_count	5

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	5,6,7,9,8

GVPGALSNQPAPPNEAPIATP

Entity 5 Entity 5 polymer

Description description	Flagellar basal-body rod protein FlgC
Molecular weight formula_weight	13991.9 kDa
Source method src_method	nat
Copy count entity_count	6

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	f,g,h,j,p,i

MALLNIFDIAGSALAAQSKRLNVAASNLANADSVTGPDGQPYRAKQVVFQVDAAPGQATGGVKVASVIESQAPEKLVYEPGNPLADANGYVKMPNVDVVGEMVNTMSASRSYQANIEVLNTVKSMMLKTLTLGQ

Entity 6 Entity 6 polymer

Description description	Flagellar basal body rod protein FlgB
Molecular weight formula_weight	15145.1 kDa
Source method src_method	nat
Copy count entity_count	5

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	l,m,o,k,n

MLDRLDAALRFQQEALNLRAQRQEILAANIANADTPGYQARDIDFASELKKVMVRGREETGGVALTLTSSHHIPAQAVSSPAVDLLYRVPDQPSLDGNTVDMDRERTQFADNSLKYQMGLTVLGSQLKGMMNVLQGGN

Entity 7 Entity 7 polymer

Description description	Flagellar hook-basal body complex protein FliE
Molecular weight formula_weight	11087.7 kDa
Source method src_method	nat
Copy count entity_count	6

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	q,r,s,t,u,v

MAAIQGIEGVISQLQATAMAARGQDTHSQSTVSFAGQLHAALDRISDRQAAARVQAEKFTLGEPGIALNDVMADMQKASVSMQMGIQVRNKLVAAYQEVMSMQV

Entity 8 Entity 8 polymer

Description description	Flagellar hook protein FlgE
Molecular weight formula_weight	42233.2 kDa
Source method src_method	nat
Copy count entity_count	11

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	DA,DB,DC,DD,DE,DF,DG,DH,DI,DJ,DK

MSFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFAGSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKLDENRNLVNMQGMQLTGYPATGTPPTIQQGANPAPITIPNTLMAAKSTTTASMQINLNSTDPVPSKTPFSVSDADSYNKKGTVTVYDSQGNAHDMNVYFVKTKDNEWAVYTHDSSDPAATAPTTASTTLKFNENGILESGGTVNITTGTINGATAATFSLSFLNSMQQNTGANNIVATNQNGYKPGDLVSYQINNDGTVVGNYSNEQEQVLGQIVLANFANNEGLASQGDNVWAATQASGVALLGTAGSGNFGKLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQILNTLVNLR

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Unit-cell length a length_a	1.000 Å
Unit-cell length b length_b	1.000 Å
Unit-cell length c length_c	1.000 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	—

Space group space_group	P 1
Space-group number int_tables_number	1
Crystal system crystal_system	—

Resolution resolution	3.30 Å
Resolution测定Method resolution_method	—
颗粒数 num_particles	102044

Cryo-EM structure of the flagellar rod with partial hook from Salmonella

1. Protein Identity and Related Structures Protein Identity & Related Structures

Flagellar basal-body rod protein FlgG

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Flagellar basal-body rod protein FlgF

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Flagellar MS ring L1

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Flagellar basal-body rod protein FlgC

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Flagellar basal body rod protein FlgB

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Flagellar hook-basal body complex protein FliE

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Flagellar hook protein FlgE

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

7e82__assembly_1__model_1

7e82__assembly_1__model_1 | I(q)

7e82__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

冷冻电镜 EM 3D

5. Entities and Polymers Entities & Polymers (8)

Entity 1 Entity 1 polymer

Polymer Polymer

Entity 2 Entity 2 polymer

Polymer Polymer

Entity 3 Entity 3 polymer

Polymer Polymer

Entity 4 Entity 4 polymer

Polymer Polymer

Entity 5 Entity 5 polymer

Polymer Polymer

Entity 6 Entity 6 polymer

Polymer Polymer