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8wk3

Cryo-EM structure of the proximal rod-export apparatus and FlgF within the motor-hook complex in the CW state

Method: ELECTRON MICROSCOPY
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Flagellar hook-basal body complex protein FliE

OrganismNot specified

UniProt P26462

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 43 Flagellar basal body rod protein FlgB × 5 （P16437） Flagellar biosynthetic protein FliR × 1 （P54702） Flagellar biosynthetic protein FliP × 5 （P54700） Flagellar biosynthetic protein FliQ × 4 （P0A1L5） Flagellar M-ring protein × 11 （P15928） Flagellar basal-body rod protein FlgC × 6 （P0A1I7） Flagellar basal-body rod protein FlgF × 5 （P16323） Consistent with protein count

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
7bin 7cg4 7cgo 7e80 7e82 8wk3 8wk4 8wkk 8wkq 8wl2 8wlh 8wln

16 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name FLIE_SALTY
Isoform —
PDB entities 1
Chains and sequence ranges Author chain K； PDBConstruct 1–104； UniProt 1–104 Author chain L； PDBConstruct 1–104； UniProt 1–104 Author chain M； PDBConstruct 1–104； UniProt 1–104 Author chain N； PDBConstruct 1–104； UniProt 1–104 Author chain O； PDBConstruct 1–104； UniProt 1–104 Author chain P； PDBConstruct 1–104； UniProt 1–104

Flagellar basal body rod protein FlgB

OrganismNot specified

UniProt P16437

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 43 Flagellar hook-basal body complex protein FliE × 6 （P26462） Flagellar biosynthetic protein FliR × 1 （P54702） Flagellar biosynthetic protein FliP × 5 （P54700） Flagellar biosynthetic protein FliQ × 4 （P0A1L5） Flagellar M-ring protein × 11 （P15928） Flagellar basal-body rod protein FlgC × 6 （P0A1I7） Flagellar basal-body rod protein FlgF × 5 （P16323） Consistent with protein count

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
7bin 7cg0 7cgo 7e80 7e82 8wk3 8wk4 8wkk 8wkq 8wl2 8wlh 8wln

16 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name FLGB_SALTY
Isoform —
PDB entities 2
Chains and sequence ranges Author chain Q； PDBConstruct 1–138； UniProt 1–138 Author chain R； PDBConstruct 1–138； UniProt 1–138 Author chain S； PDBConstruct 1–138； UniProt 1–138 Author chain T； PDBConstruct 1–138； UniProt 1–138 Author chain U； PDBConstruct 1–138； UniProt 1–138

Flagellar biosynthetic protein FliR

OrganismNot specified

UniProt P54702

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 43 Flagellar hook-basal body complex protein FliE × 6 （P26462） Flagellar basal body rod protein FlgB × 5 （P16437） Flagellar biosynthetic protein FliP × 5 （P54700） Flagellar biosynthetic protein FliQ × 4 （P0A1L5） Flagellar M-ring protein × 11 （P15928） Flagellar basal-body rod protein FlgC × 6 （P0A1I7） Flagellar basal-body rod protein FlgF × 5 （P16323） Consistent with protein count

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
6f2d 6r69 7cgo 7e80 8wk3 8wkk 8wkq 8wl2 8wlh 8wln 8wlq 8wlt

15 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name FLIR_SALTY
Isoform —
PDB entities 3
Chains and sequence ranges Author chain E； PDBConstruct 1–264； UniProt 1–264

Flagellar biosynthetic protein FliP

OrganismNot specified

UniProt P54700

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 43 Flagellar hook-basal body complex protein FliE × 6 （P26462） Flagellar basal body rod protein FlgB × 5 （P16437） Flagellar biosynthetic protein FliR × 1 （P54702） Flagellar biosynthetic protein FliQ × 4 （P0A1L5） Flagellar M-ring protein × 11 （P15928） Flagellar basal-body rod protein FlgC × 6 （P0A1I7） Flagellar basal-body rod protein FlgF × 5 （P16323） Consistent with protein count

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
7cg4 7cgo 7e80 8wk3 8wkk 8wkq 8wl2 8wlh 8wln 8wlq 8wlt 8wo5

14 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name FLIP_SALTY
Isoform —
PDB entities 4
Chains and sequence ranges Author chain F； PDBConstruct 1–245； UniProt 1–245 Author chain G； PDBConstruct 1–245； UniProt 1–245 Author chain H； PDBConstruct 1–245； UniProt 1–245 Author chain I； PDBConstruct 1–245； UniProt 1–245 Author chain J； PDBConstruct 1–245； UniProt 1–245

Flagellar biosynthetic protein FliQ

OrganismNot specified

UniProt P0A1L5

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 43 Flagellar hook-basal body complex protein FliE × 6 （P26462） Flagellar basal body rod protein FlgB × 5 （P16437） Flagellar biosynthetic protein FliR × 1 （P54702） Flagellar biosynthetic protein FliP × 5 （P54700） Flagellar M-ring protein × 11 （P15928） Flagellar basal-body rod protein FlgC × 6 （P0A1I7） Flagellar basal-body rod protein FlgF × 5 （P16323） Consistent with protein count

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
7cgo 7e80 8wk3 8wkk 8wkq 8wl2 8wlh 8wln 8wlq 8wlt 8wo5 8woe

13 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name FLIQ_SALTY
Isoform —
PDB entities 5
Chains and sequence ranges Author chain A； PDBConstruct 1–89； UniProt 1–89 Author chain B； PDBConstruct 1–89； UniProt 1–89 Author chain C； PDBConstruct 1–89； UniProt 1–89 Author chain D； PDBConstruct 1–89； UniProt 1–89

Flagellar M-ring protein

OrganismNot specified

UniProt P15928

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 43 Flagellar hook-basal body complex protein FliE × 6 （P26462） Flagellar basal body rod protein FlgB × 5 （P16437） Flagellar biosynthetic protein FliR × 1 （P54702） Flagellar biosynthetic protein FliP × 5 （P54700） Flagellar biosynthetic protein FliQ × 4 （P0A1L5） Flagellar basal-body rod protein FlgC × 6 （P0A1I7） Flagellar basal-body rod protein FlgF × 5 （P16323） Consistent with protein count

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer

None

Other homooligomers
6sd1 6sd2 6sd3 6sd4 6sd5 6tre 7bk0 7cg7 7d84 8fte 8ftf 8t8p

17 assemblies; showing the first 12

Heteromeric or nucleic-acid complex
7cg0 7cgo 7e80 7e81 7e82 7nvg 8umd 8umx 8uox 8upl 8vib 8vid

31 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name FLIF_SALTY
Isoform —
PDB entities 6
Chains and sequence ranges Author chain b； PDBConstruct 1–560； UniProt 1–560 Author chain c； PDBConstruct 1–560； UniProt 1–560 Author chain d； PDBConstruct 1–560； UniProt 1–560 Author chain e； PDBConstruct 1–560； UniProt 1–560 Author chain f； PDBConstruct 1–560； UniProt 1–560 Author chain g； PDBConstruct 1–560； UniProt 1–560 Author chain h； PDBConstruct 1–560； UniProt 1–560 Author chain i； PDBConstruct 1–560； UniProt 1–560 Author chain j； PDBConstruct 1–560； UniProt 1–560 Author chain k； PDBConstruct 1–560； UniProt 1–560 Author chain l； PDBConstruct 1–560； UniProt 1–560

Flagellar basal-body rod protein FlgC

OrganismNot specified

UniProt P0A1I7

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 43 Flagellar hook-basal body complex protein FliE × 6 （P26462） Flagellar basal body rod protein FlgB × 5 （P16437） Flagellar biosynthetic protein FliR × 1 （P54702） Flagellar biosynthetic protein FliP × 5 （P54700） Flagellar biosynthetic protein FliQ × 4 （P0A1L5） Flagellar M-ring protein × 11 （P15928） Flagellar basal-body rod protein FlgF × 5 （P16323） Consistent with protein count

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
7bin 7cg0 7cgo 7e80 7e82 8wk3 8wkk 8wkq 8wl2 8wlh 8wln 8wlq

15 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name FLGC_SALTY
Isoform —
PDB entities 7
Chains and sequence ranges Author chain V； PDBConstruct 1–134； UniProt 1–134 Author chain W； PDBConstruct 1–134； UniProt 1–134 Author chain X； PDBConstruct 1–134； UniProt 1–134 Author chain Y； PDBConstruct 1–134； UniProt 1–134 Author chain Z； PDBConstruct 1–134； UniProt 1–134 Author chain a； PDBConstruct 1–134； UniProt 1–134

Flagellar basal-body rod protein FlgF

OrganismNot specified

UniProt P16323

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 43 Flagellar hook-basal body complex protein FliE × 6 （P26462） Flagellar basal body rod protein FlgB × 5 （P16437） Flagellar biosynthetic protein FliR × 1 （P54702） Flagellar biosynthetic protein FliP × 5 （P54700） Flagellar biosynthetic protein FliQ × 4 （P0A1L5） Flagellar M-ring protein × 11 （P15928） Flagellar basal-body rod protein FlgC × 6 （P0A1I7） Consistent with protein count

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
7bin 7cbm 7cgo 7e80 7e82 8wk3 8wkk 8wl2 8wlh 8wlq 8wlt 8wo5

13 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name FLGF_SALTY
Isoform —
PDB entities 8
Chains and sequence ranges Author chain m； PDBConstruct 1–251； UniProt 1–251 Author chain n； PDBConstruct 1–251； UniProt 1–251 Author chain o； PDBConstruct 1–251； UniProt 1–251 Author chain p； PDBConstruct 1–251； UniProt 1–251 Author chain q； PDBConstruct 1–251； UniProt 1–251

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id8wk3
Deposition date deposition_date2023-09-26
Structure title titleCryo-EM structure of the proximal rod-export apparatus and FlgF within the motor-hook complex in the CW state
Keywords keywordsFlagellum, Flagellar motor, Proximal rod, Export apparatus, FlgF, MOTOR PROTEIN; MOTOR PROTEIN
Experimental Method methodELECTRON MICROSCOPY
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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

8wk3__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

8wk3__assembly_1__model_1 | I(q)

10-2 10-1 106 107 108 109 q (1/Angstrom) I(q)
100 plotted points; both axes use logarithmic scales.

8wk3__assembly_1__model_1 | P(r) · Pending

The new assembly/model P(r) has not been calculated yet This placeholder does not display legacy data r (Angstrom) P(r)
P(r) will be calculated and displayed separately for the same assembly/model.
Rg（Guinier）63.09 Å
Rg (electron density)64.70 Å
Total Rg64.54 Å
Atom count36243
Residues4847
Excluded volume653030 ų
Maximum q0.500 Å⁻¹
Assembly Model Structure unit Oligomeric description Status CRYSOL Actions
1 1 8wk3__assembly_1__model_1 43-meric （43） Success 4.1.3-1-20251215 (887e7ef) View Download
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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (8)

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7. Citations (1)