Outer membrane protein assembly factor BamA
Escherichia coli
State in the Current Structure
| Assembly | Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Associated Components | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|
| 1 | Protein heterocomplex Heteromer Protein × 5 PDB declaration: pentameric(5) Consistent with protein copy count | Chain A; UniProt 92–810 | Not recorded | Outer membrane protein assembly factor BamB × 1 (P77774) Outer membrane protein assembly factor BamC × 1 (P0A903) Outer membrane protein assembly factor BamD × 1 (P0AC04) Outer membrane protein assembly factor BamE × 1 (P0A938) | ELECTRON MICROSCOPY cryo-EM buffer:pH 8 cryo-EM vitrification conditions:Cryogen ETHANE | Resolution 4.30 Å |
Other States of the Same Protein in the Database
Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.
| Other PDB | Difference from Current Entry 9H84 | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 2QCZ Structure of N-terminal domain of E. Coli YaeT Deposited 2007-06-20 | Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric |
Chain A
21–351(331 aa)
Fragment:residues 21-351
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 4.8;295 K;10% PEG 10K, 100mM sodium acetate, 100 mM magnesium formate, 10%glycerol, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 2.70 Å R-free 0.329 |
| 2QCZ Structure of N-terminal domain of E. Coli YaeT Deposited 2007-06-20 | Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric |
Chain B
21–351(331 aa)
Fragment:residues 21-351
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 4.8;295 K;10% PEG 10K, 100mM sodium acetate, 100 mM magnesium formate, 10%glycerol, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 2.70 Å R-free 0.329 |
| 2QDF Structure of N-terminal domain of E. Coli YaeT Deposited 2007-06-20 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric |
Chain A
21–351(331 aa)
Fragment:residues 21-351
|
Not recorded | MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 4.8;295 K;10% PEG 10K, 100mM sodium acetate, 100 mM magnesium formate, 10%glycerol, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 2.20 Å R-free 0.286 |
| 2V9H Solution Structure of an Escherichia coli YaeT tandem POTRA domain Deposited 2007-08-23 | Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric |
Chain A
21–174(154 aa)
Fragment:POTRA DOMAINS 1 AND 2, RESIDUES 21-174
|
Not recorded | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 6.5;303 K;Ionic strength (raw mmCIF value) 86;Pressure 1.0
NMR sample composition
50 MM MES, 50 MM NACL
|
Resolution not provided |
| 3EFC Crystal Structure of YaeT periplasmic domain Deposited 2008-09-08 | Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric |
Chain A
21–410(390 aa)
Fragment:periplasmic domain (UNP residues 21-410)
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 6.5;289.15 K;1.3-1.6M (NH4)2SO4, 3-6% polyethylene glycol 400, 10% Dioxane, 0.1M MES pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289.15K
|
Resolution 3.30 Å R-free 0.295 |
| 4C4V Structure of the outer membrane protein insertase BamA with one POTRA domain. Deposited 2013-09-09 | Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric |
Chain A
347–810(464 aa)
Fragment:BAMA TRUNCATED BY 4 POTRA DOMAINS, RESIDUES 347-808
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7.5;pH 7.5
|
Resolution 3.00 Å R-free 0.292 |
| 4C4V Structure of the outer membrane protein insertase BamA with one POTRA domain. Deposited 2013-09-09 | Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric |
Chain B
344–810(467 aa)
Fragment:BAMA TRUNCATED BY 4 POTRA DOMAINS, RESIDUES 344-808
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7.5;pH 7.5
|
Resolution 3.00 Å R-free 0.292 |
| 4N75 Structural Basis of BamA-mediate Outer Membrane Protein Biogenesis Deposited 2013-10-14 | Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric |
Chain A
427–810(384 aa)
Fragment:barrel domain, UNP residues 427-810
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 6;289 K;0.1M MES PH 6.0, 6% Tacsimate, 25% PEG 4000, 0.6% C8E4, VAPOR DIFFUSION, HANGING DROP, temperature 289K
|
Resolution 2.60 Å R-free 0.253 |
| 4N75 Structural Basis of BamA-mediate Outer Membrane Protein Biogenesis Deposited 2013-10-14 | Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric |
Chain B
427–810(384 aa)
Fragment:barrel domain, UNP residues 427-810
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 6;289 K;0.1M MES PH 6.0, 6% Tacsimate, 25% PEG 4000, 0.6% C8E4, VAPOR DIFFUSION, HANGING DROP, temperature 289K
|
Resolution 2.60 Å R-free 0.253 |
| 4PK1 Structure of BamB fused to a BamA POTRA domain fragment Deposited 2014-05-13 | Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Monomer;Protein × 1 PDB declaration: monomeric |
Chain A
175–424(250 aa)
Fragment:UNP P0A940 residues 175-424,UNP P77774 residues 21-392
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;289 K;The BamA-BamB chimera protein was crystallized at 16 degC by sitting drop vapor diffusion method. Initial crystallizing conditions were refined in a hanging drop in the following solution: 1.4M Ammonium sulfate, 9% 2-propanol, and 0.1M Sodium acetate pH 5.5. We used 1.5 uL of the 20mg/ml protein mixed with 1.5 uL mother liquor in a reservoir filled with 500 uL precipitant solution
|
Resolution 3.10 Å R-free 0.281 |
| 4XGA Crystal structure of BamB and BamA P3-5 complex from E.coli Deposited 2014-12-30 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric |
Chain B
175–420(246 aa)
Fragment:UNP RESIDUES 175-420
|
Not recorded | CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP;pH 7;293 K;1% w/v Tryptone, 20% w/v Polyethylene glycol 3350, 0.05M HEPES sodium pH 7.0
|
Resolution 2.15 Å R-free 0.231 |
| 5AYW Structure of a membrane complex Deposited 2015-09-14 | Different construct Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer;Protein × 5 PDB declaration: pentameric |
Chain A
22–810(789 aa)
Fragment:UNP residues 22-810
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION;289 K;Tris-HCl, NaCl, PEG 400
|
Resolution 3.56 Å R-free 0.314 |
| 5D0O BamABCDE complex, outer membrane beta barrel assembly machinery entire complex Deposited 2015-08-03 | Different construct Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer;Protein × 5 PDB declaration: pentameric |
Chain A
1–810(810 aa)
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP;pH 7.5;293 K;0.1 M HEPES, pH7.5, 30% PEG600, CYMAL-4
|
Resolution 2.90 Å R-free 0.304 |
| 5D0Q BamACDE complex, outer membrane beta-barrel assembly machinery (BAM) complex Deposited 2015-08-03 | Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer;Protein × 4 PDB declaration: tetrameric |
Chain A
2–810(809 aa)
|
Non-standard monomer:Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP;pH 8;297 K;0.1 M Tris, pH8.0, 0.1 M MgCl2, 24% PEG500 MME.
|
Resolution 3.50 Å R-free 0.340 |
| 5D0Q BamACDE complex, outer membrane beta-barrel assembly machinery (BAM) complex Deposited 2015-08-03 | Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer;Protein × 4 PDB declaration: tetrameric |
Chain F
2–810(809 aa)
|
Non-standard monomer:Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP;pH 8;297 K;0.1 M Tris, pH8.0, 0.1 M MgCl2, 24% PEG500 MME.
|
Resolution 3.50 Å R-free 0.340 |
| 5EKQ The structure of the BamACDE subcomplex from E. coli Deposited 2015-11-04 | Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer;Protein × 4 PDB declaration: tetrameric |
Chain A
21–810(790 aa)
Fragment:UNP residues 21-810
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 8.5;294 K;100 mM Tris-HCl, pH 8.5, 200 mM MgCl2, 10 mM MnCl2, and 8% PEG 4000
|
Resolution 3.39 Å R-free 0.281 |
| 6LYQ Structure of the BAM complex Deposited 2020-02-15 | Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer;Protein × 6 PDB declaration: hexameric |
Chain A
1–810(810 aa)
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;289 K;27-30% PEG 400, 100mM NaCl
|
Resolution 3.19 Å R-free 0.293 |
| 6LYR Structure of the BAM complex Deposited 2020-02-15 | Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer;Protein × 6 PDB declaration: hexameric |
Chain A
1–810(810 aa)
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;289 K;27-30% PEG 400, 100mM NaCl
|
Resolution 3.28 Å R-free 0.280 |
| 6LYS Structure of the BAM complex Deposited 2020-02-15 | Different construct Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer;Protein × 5 PDB declaration: pentameric |
Chain A
1–810(810 aa)
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;289 K;27-30% PEG 400, 100mM NaCl
|
Resolution 3.05 Å R-free 0.284 |
| 6LYU Structure of the BAM complex Deposited 2020-02-15 | Different construct Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer;Protein × 5 PDB declaration: pentameric |
Chain A
1–810(810 aa)
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.20 Å |
| 6T1W Structure of E. coli BamA in complex with lipoprotein RcsF Deposited 2019-10-07 | Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric |
Chain A
1–810(810 aa)
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP;pH 8.5;293 K;0.03 M sodium nitrate, 0.03 M sodium phosphate dibasic, 0.03 M ammonium sulfate, 0.10 M Tris-base [pH 8.5]; BICINE, 20 % (v/v) ethylene glycol; 10 % w/v PEG 8000
|
Resolution 3.79 Å R-free 0.317 |
| 6T1W Structure of E. coli BamA in complex with lipoprotein RcsF Deposited 2019-10-07 | Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric |
Chain B
1–810(810 aa)
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP;pH 8.5;293 K;0.03 M sodium nitrate, 0.03 M sodium phosphate dibasic, 0.03 M ammonium sulfate, 0.10 M Tris-base [pH 8.5]; BICINE, 20 % (v/v) ethylene glycol; 10 % w/v PEG 8000
|
Resolution 3.79 Å R-free 0.317 |
| 6V05 Cryo-EM structure of a substrate-engaged Bam complex Deposited 2019-11-18 | Different construct Different mutation/modification Different oligomeric state Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer;Protein × 6 PDB declaration: hexameric |
Chain A
1–810(810 aa)
Chain F
1–21(21 aa)
Chain F
22–171(150 aa)
Chain F
422–429(8 aa)
Chain F
440–810(371 aa)
|
Mutation:C690S, C700S, F804C Mutation:T467C,C690S,C700S,deletion of residues 172-421,deletion of residues 430-439 Mutation:T467C,C690S,C700S,deletion of residues 172-421,deletion of residues 430-439 Mutation:T467C,C690S,C700S,deletion of residues 172-421,deletion of residues 430-439 Mutation:T467C,C690S,C700S,deletion of residues 172-421,deletion of residues 430-439 | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.10 Å |
| 7BNQ Lateral-closed conformation of the lid-locked BAM complex (BamA E435C S665C, BamBDCE) by cryoEM Deposited 2021-01-22 | Different construct Different mutation/modification Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer;Protein × 5 PDB declaration: pentameric |
Chain A
1–810(810 aa)
|
Mutation:E435C S665C C690S C700S | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.10 Å |
| 7NBX Lateral-open conformation of the lid-locked BAM complex (BamA E435C S665C, BamBDCE) by cryoEM Deposited 2021-01-28 | Different construct Different mutation/modification Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer;Protein × 5 PDB declaration: pentameric |
Chain A
1–810(810 aa)
|
Mutation:E435C S665C C690S C700S | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.80 Å |
| 7NCS Lateral-open conformation of the lid-locked BAM complex (BamA E435C S665C, BamBDCE) bound by a bactericidal Fab fragment Deposited 2021-01-29 | Different construct Different mutation/modification Different oligomeric state Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer;Protein × 7 PDB declaration: heptameric |
Chain A
1–810(810 aa)
|
Mutation:E435C S665C C690S C700S | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 7.10 Å |
| 7ND0 lateral-open conformation of the wild-type BAM complex (BamABCDE) bound to a bactericidal Fab fragment Deposited 2021-01-29 | Different construct Different oligomeric state Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer;Protein × 7 PDB declaration: heptameric |
Chain A
1–810(810 aa)
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.20 Å |
| 7NRI Structure of the darobactin-bound E. coli BAM complex (BamABCDE) Deposited 2021-03-03 | Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer;Protein × 6 PDB declaration: hexameric |
Chain A
21–808(788 aa)
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE;4microL of sample applied
|
Resolution 3.03 Å |
| 7R1W E. coli BAM complex (BamABCDE) bound to dynobactin A Deposited 2022-02-03 | Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer;Protein × 6 PDB declaration: hexameric |
Chain A
1–809(809 aa)
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8;20 mM Tris pH 8.0, 150 mM NaCl, 0.05% (w/v) n-dodecyl Beta-D-maltopyranoside (DDM)
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 7RI4 Structure of a BAM/EspP(beta9-12) hybrid-barrel intermediate Deposited 2021-07-19 | Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer;Protein × 6 PDB declaration: hexameric |
Chain A
1–810(810 aa)
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 7RI5 Structure of a BAM in MSP1E3D1 nanodiscs at 4 Angstrom resolution Deposited 2021-07-19 | Different construct Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer;Protein × 5 PDB declaration: pentameric |
Chain A
1–810(810 aa)
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.00 Å |
| 7RI6 Structure of BAM in MSP1E3D1 nanodiscs prepared from E. coli outer membranes Deposited 2021-07-19 | Different construct Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer;Protein × 5 PDB declaration: pentameric |
Chain A
1–810(810 aa)
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.90 Å |
| 7RI7 The structure of BAM in MSP1D1 nanodiscs Deposited 2021-07-19 | Different construct Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer;Protein × 5 PDB declaration: pentameric |
Chain A
1–810(810 aa)
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 8.00 Å |
| 7RI8 The structure of BAM in MSP2N2 nanodiscs Deposited 2021-07-19 | Different construct Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer;Protein × 5 PDB declaration: pentameric |
Chain A
1–810(810 aa)
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 7.50 Å |
| 7RI9 The structure of BAM in MSP1E3D1 at 6.9 Angstrom resolution Deposited 2021-07-19 | Different construct Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer;Protein × 5 PDB declaration: pentameric |
Chain A
1–810(810 aa)
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.90 Å |
| 7RJ5 The structure of BAM in complex with EspP at 7 Angstrom resolution Deposited 2021-07-20 | Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer;Protein × 6 PDB declaration: hexameric |
Chain A
1–810(810 aa)
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 7.00 Å |
| 7YE4 BAM-EspP complex structure with BamA-G431C and G781C/EspP-N1293C and A1043C mutations in nanodisc Deposited 2022-07-05 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer;Protein × 6 PDB declaration: hexameric |
Chain A
1–810(810 aa)
|
Mutation:G431C,G781C | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.8;20 mM Tris-HCl, pH 7.8, 150 mM NaCl
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 7YE6 BAM-EspP complex structure with BamA-N427C/EspP-R1297C mutations in nanodisc Deposited 2022-07-05 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer;Protein × 6 PDB declaration: hexameric |
Chain A
1–810(810 aa)
|
Mutation:N427C | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.8;20 mM Tris-HCl, pH 7.8, 150 mM NaCl
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 8ADG Cryo-EM structure of Darobactin 22 bound BAM complex Deposited 2022-07-08 | Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer;Protein × 6 PDB declaration: hexameric |
Chain A
1–810(810 aa)
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 3.00 Å |
| 8ADI Cryo-EM structure of Darobactin 9 bound BAM complex Deposited 2022-07-08 | Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer;Protein × 6 PDB declaration: hexameric |
Chain A
1–810(810 aa)
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 3.40 Å |
| 8BNZ BAM-EspP complex structure with BamA-G431C/EspP-N1293C mutations in nanodisc Deposited 2022-11-14 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer;Protein × 6 PDB declaration: hexameric |
Chain A
1–810(810 aa)
|
Mutation:G431C | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 8BVQ E. coli BAM complex (BamABCDE) bound to darobactin B Deposited 2022-12-05 | Different construct Different oligomeric state Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer;Protein × 6 PDB declaration: hexameric |
Chain A
21–810(790 aa)
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 8PZ1 Wait Complex: Lateral open BAM bound Compact SurA Deposited 2023-07-26 | Different construct Different mutation/modification Different oligomeric state Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer;Protein × 6 PDB declaration: hexameric |
Chain A
21–810(790 aa)
|
Mutation:R76C | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.10 Å |
| 8PZ2 Wait Complex: Lateral open BAM bound Extended SurA Deposited 2023-07-26 | Different construct Different mutation/modification Different oligomeric state Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer;Protein × 6 PDB declaration: hexameric |
Chain A
21–810(790 aa)
|
Mutation:R76C | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.20 Å |
| 8PZU Wait Complex: BAM bound Darobactin-B and Compact SurA Deposited 2023-07-27 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer;Protein × 7 PDB declaration: heptameric |
Chain A
21–810(790 aa)
|
Mutation:R76C | MG MAGNESIUM ION × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 8Q0G Release Complex: BAM bound EspP and Compact SurA Deposited 2023-07-28 | Different construct Different mutation/modification Different oligomeric state | Assembly 1 Protein heterocomplex Heteromer;Protein × 7 PDB declaration: heptameric |
Chain A
21–810(790 aa)
|
Mutation:S425C | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.30 Å |
| 8QN4 Structure of the BAM-EspP complex in an open EspP intermediate state Deposited 2023-09-25 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer;Protein × 6 PDB declaration: hexameric |
Chain A
1–810(810 aa)
|
Mutation:G431C | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.36 Å |
| 8QP5 Release Complex: BAM bound EspP (SurA released) Deposited 2023-09-29 | Different construct Different mutation/modification Different oligomeric state Different structure-quality metrics | Assembly 1 Insufficient information Heteromer;Protein × 6 PDB declaration: hexameric |
Chain A
21–810(790 aa)
|
Mutation:S425C | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.40 Å |
| 8QPU Release Complex: BAM bound EspP and Extended SurA Deposited 2023-10-03 | Different construct Different mutation/modification Different oligomeric state Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer;Protein × 7 PDB declaration: heptameric |
Chain A
21–810(790 aa)
|
Mutation:S425C | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.20 Å |
| 8QPV Handover Complex: BAM bound OmpX and extended SurA Deposited 2023-10-03 | Different construct Different mutation/modification Different oligomeric state Different structure-quality metrics | Assembly 1 Insufficient information Heteromer;Protein × 6 PDB declaration: heptameric |
Chain A
21–810(790 aa)
|
Mutation:S425C | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.00 Å |
| 8QPW Arrival Complex: Lateral open BAM bound extended SurA plus OmpX Deposited 2023-10-03 | Different construct Different oligomeric state Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer;Protein × 7 PDB declaration: heptameric |
Chain A
21–810(790 aa)
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.30 Å |
| 9CNW CryoEM structure of the APO-BAM complex in DDM detergent Deposited 2024-07-15 | Different construct Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer;Protein × 5 PDB declaration: pentameric |
Chain A
1–810(810 aa)
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.60 Å |
| 9CNX CryoEM structure of BAM in complex with the PTB1 closed-state inhibitor (in DDM detergent) Deposited 2024-07-15 | Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer;Protein × 6 PDB declaration: hexameric |
Chain A
1–810(810 aa)
|
Not recorded | ZN ZINC ION × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.55 Å |
| 9CNY CryoEM structure of the APO-BAM complex in SMA nanodisc Deposited 2024-07-15 | Different construct Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer;Protein × 5 PDB declaration: pentameric |
Chain A
1–810(810 aa)
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.00 Å |
| 9CNZ Structure of BAM complexed with PTB2 ligand in detergent Deposited 2024-07-15 | Different construct Different oligomeric state Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer;Protein × 8 PDB declaration: octameric |
Chain A
1–810(810 aa)
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 9CO0 CryoEM structure of BAM in complex with the PTB2 open-state inhibitor (in SMA nanodisc) Deposited 2024-07-15 | Different construct Different oligomeric state Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer;Protein × 8 PDB declaration: octameric |
Chain A
1–810(810 aa)
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 9H85 BAM-hinge (GSGS) Deposited 2024-10-28 | Different construct Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer;Protein × 5 PDB declaration: pentameric |
Chain A
21–810(790 aa)
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.20 Å |
| 9H89 BAM-hinge (LVPR) suppressor (T434A) Deposited 2024-10-28 | Different construct Different mutation/modification Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer;Protein × 5 PDB declaration: pentameric |
Chain A
21–810(790 aa)
|
Mutation:T434A | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.00 Å |
| 9HE1 Structure of the Xenorceptide A2-bound E. coli BAM complex (BamABCDE) Deposited 2024-11-13 | Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer;Protein × 6 PDB declaration: hexameric |
Chain A
1–810(810 aa)
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7;25 mM NaPi, 100 mM NaCl, pH 7.0
cryo-EM vitrification conditions
Cryogen ETHANE;3.5uL sample,
blot time 3sec,
Blotforce 1,
Blot total 1,
|
Resolution 3.00 Å |
| 9HG5 BAM-SurA complex in the swing-in state with full length BamC resolved Deposited 2024-11-19 | Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer;Protein × 6 PDB declaration: hexameric |
Chain A
21–810(790 aa)
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8;20mM Tris-HCl, 150mM NaCl, 0.05% DDM
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.82 Å |
| 9HG6 BAM-SurA complex in the swing-in state Deposited 2024-11-19 | Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer;Protein × 6 PDB declaration: hexameric |
Chain A
21–810(790 aa)
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8;20mM Tris-HCl, 150mM NaCl, 0.05% DDM
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.73 Å |
| 9HG7 BAM-SurA complex in the swing-out state Deposited 2024-11-19 | Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer;Protein × 6 PDB declaration: hexameric |
Chain A
21–810(790 aa)
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8;20mM Tris-HCl, 150mM NaCl, 0.05% DDM
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.83 Å |
| 9HG8 BAM-SurA complex in the swing-out state with BamC resolved Deposited 2024-11-19 | Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer;Protein × 6 PDB declaration: hexameric |
Chain A
21–810(790 aa)
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8;20mM Tris-HCl, 150mM NaCl, 0.05% DDM
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 9HG9 BAM-SurA-darobactin complex in the swing-in state Deposited 2024-11-19 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer;Protein × 7 PDB declaration: heptameric |
Chain A
21–810(790 aa)
|
Not recorded | MG MAGNESIUM ION × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8;20mM Tris-HCl, 150mM NaCl, 0.05% DDM
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.88 Å |
| 9HGA BAM-SurA-darobactin complex in the swing-out state Deposited 2024-11-19 | Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer;Protein × 7 PDB declaration: heptameric |
Chain A
21–810(790 aa)
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8;20mM Tris-HCl, 150mM NaCl, 0.05% DDM
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.62 Å |
| 9MGE beta-barrel assembly machine from Escherichia coli in an early state of substrate assembly Deposited 2024-12-10 | Different construct Different mutation/modification Different oligomeric state Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer;Protein × 7 PDB declaration: heptameric |
Chain A
24–810(787 aa)
Chain F
22–171(150 aa)
Chain F
422–810(389 aa)
|
Mutation:D512C Mutation:Y637C Mutation:Y637C | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 9MGF beta-barrel assembly machine from Escherichia coli in a middle state of substrate assembly Deposited 2024-12-10 | Different construct Different mutation/modification Different oligomeric state Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer;Protein × 6 PDB declaration: heptameric |
Chain A
21–810(790 aa)
Chain F
22–171(150 aa)
Chain F
422–810(389 aa)
|
Mutation:D512C | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 9MGG beta-barrel assembly machine from Escherichia coli in a late state of substrate assembly Deposited 2024-12-10 | Different construct Different mutation/modification Different oligomeric state Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer;Protein × 6 PDB declaration: hexameric |
Chain A
24–810(787 aa)
Chain F
22–171(150 aa)
Chain F
422–810(389 aa)
|
Mutation:D512C Mutation:I458C Mutation:I458C | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.90 Å |
| 9P1U beta-barrel assembly machine from Escherichia coli in an late state of LptD assembly Deposited 2025-06-10 | Different construct Different oligomeric state Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer;Protein × 7 PDB declaration: heptameric |
Chain A
1–810(810 aa)
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.10 Å |
64 other PDB entries and 69 assemblies. Open the comparison page and filter oligomeric states
View Construct and Data Evidence
| UniProt name | BAMA_ECOLI |
| Isoform | — |
| PDB entities | 1 |
| Chains and sequence ranges | Author chain A; PDBConstruct 1–723; UniProt 92–810 |