Type 1 fimbrin D-mannose specific adhesin
Escherichia coli
State in the Current Structure
| Assembly | Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Associated Components | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|
| 1 | Protein heterocomplex Heteromer Protein × 2 PDB declaration: dimeric（2） Consistent with protein copy count | Chain B； UniProt 22–181 | Mutation：L34K | FimH minibinder F7 × 1 | X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；0.2 M sodium malonate, pH 7.0, 20% w/v PEG3350 | Resolution 1.75 Å R-free 0.263 |
| 2 | Protein heterocomplex Heteromer Protein × 2 PDB declaration: dimeric（2） Consistent with protein copy count | Chain D； UniProt 22–181 | Mutation：L34K | FimH minibinder F7 × 1 | X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；0.2 M sodium malonate, pH 7.0, 20% w/v PEG3350 | Resolution 1.75 Å R-free 0.263 |
Other States of the Same Protein in the Database
Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.
| Other PDB | Difference from Current Entry 9Q1V | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1KIU FimH adhesin Q133N mutant-FimC chaperone complex with methyl-alpha-D-mannose Deposited 2001-12-03 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
22–300（279 aa）
|
Mutation：Q133N | MMA methyl alpha-D-mannopyranoside × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.2；297 K；ammonium sulphate, pH 8.2, VAPOR DIFFUSION, SITTING DROP, temperature 297K
|
Resolution 3.00 Å R-free 0.278 |
| 1KIU FimH adhesin Q133N mutant-FimC chaperone complex with methyl-alpha-D-mannose Deposited 2001-12-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 10 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
22–300（279 aa）
Chain H
22–300（279 aa）
Chain L
22–300（279 aa）
Chain P
22–300（279 aa）
|
Mutation：Q133N Mutation：Q133N Mutation：Q133N Mutation：Q133N | MMA methyl alpha-D-mannopyranoside × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.2；297 K；ammonium sulphate, pH 8.2, VAPOR DIFFUSION, SITTING DROP, temperature 297K
|
Resolution 3.00 Å R-free 0.278 |
| 1KIU FimH adhesin Q133N mutant-FimC chaperone complex with methyl-alpha-D-mannose Deposited 2001-12-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 11 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain F
22–300（279 aa）
Chain N
22–300（279 aa）
|
Mutation：Q133N Mutation：Q133N | MMA methyl alpha-D-mannopyranoside × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.2；297 K；ammonium sulphate, pH 8.2, VAPOR DIFFUSION, SITTING DROP, temperature 297K
|
Resolution 3.00 Å R-free 0.278 |
| 1KIU FimH adhesin Q133N mutant-FimC chaperone complex with methyl-alpha-D-mannose Deposited 2001-12-03 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain D
22–300（279 aa）
|
Mutation：Q133N | MMA methyl alpha-D-mannopyranoside × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.2；297 K；ammonium sulphate, pH 8.2, VAPOR DIFFUSION, SITTING DROP, temperature 297K
|
Resolution 3.00 Å R-free 0.278 |
| 1KIU FimH adhesin Q133N mutant-FimC chaperone complex with methyl-alpha-D-mannose Deposited 2001-12-03 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain F
22–300（279 aa）
|
Mutation：Q133N | MMA methyl alpha-D-mannopyranoside × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.2；297 K；ammonium sulphate, pH 8.2, VAPOR DIFFUSION, SITTING DROP, temperature 297K
|
Resolution 3.00 Å R-free 0.278 |
| 1KIU FimH adhesin Q133N mutant-FimC chaperone complex with methyl-alpha-D-mannose Deposited 2001-12-03 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain H
22–300（279 aa）
|
Mutation：Q133N | MMA methyl alpha-D-mannopyranoside × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.2；297 K；ammonium sulphate, pH 8.2, VAPOR DIFFUSION, SITTING DROP, temperature 297K
|
Resolution 3.00 Å R-free 0.278 |
| 1KIU FimH adhesin Q133N mutant-FimC chaperone complex with methyl-alpha-D-mannose Deposited 2001-12-03 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 5 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain J
22–300（279 aa）
|
Mutation：Q133N | MMA methyl alpha-D-mannopyranoside × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.2；297 K；ammonium sulphate, pH 8.2, VAPOR DIFFUSION, SITTING DROP, temperature 297K
|
Resolution 3.00 Å R-free 0.278 |
| 1KIU FimH adhesin Q133N mutant-FimC chaperone complex with methyl-alpha-D-mannose Deposited 2001-12-03 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 6 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain L
22–300（279 aa）
|
Mutation：Q133N | MMA methyl alpha-D-mannopyranoside × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.2；297 K；ammonium sulphate, pH 8.2, VAPOR DIFFUSION, SITTING DROP, temperature 297K
|
Resolution 3.00 Å R-free 0.278 |
| 1KIU FimH adhesin Q133N mutant-FimC chaperone complex with methyl-alpha-D-mannose Deposited 2001-12-03 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 7 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain N
22–300（279 aa）
|
Mutation：Q133N | MMA methyl alpha-D-mannopyranoside × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.2；297 K；ammonium sulphate, pH 8.2, VAPOR DIFFUSION, SITTING DROP, temperature 297K
|
Resolution 3.00 Å R-free 0.278 |
| 1KIU FimH adhesin Q133N mutant-FimC chaperone complex with methyl-alpha-D-mannose Deposited 2001-12-03 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 8 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain P
22–300（279 aa）
|
Mutation：Q133N | MMA methyl alpha-D-mannopyranoside × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.2；297 K；ammonium sulphate, pH 8.2, VAPOR DIFFUSION, SITTING DROP, temperature 297K
|
Resolution 3.00 Å R-free 0.278 |
| 1KIU FimH adhesin Q133N mutant-FimC chaperone complex with methyl-alpha-D-mannose Deposited 2001-12-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 9 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain D
22–300（279 aa）
Chain J
22–300（279 aa）
|
Mutation：Q133N Mutation：Q133N | MMA methyl alpha-D-mannopyranoside × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.2；297 K；ammonium sulphate, pH 8.2, VAPOR DIFFUSION, SITTING DROP, temperature 297K
|
Resolution 3.00 Å R-free 0.278 |
| 1KLF FIMH ADHESIN-FIMC CHAPERONE COMPLEX WITH D-MANNOSE Deposited 2001-12-11 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
22–300（279 aa）
|
Not recorded | MAN alpha-D-mannopyranose × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.2；298 K；ammonium suplhate, pH 8.20, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 2.79 Å R-free 0.280 |
| 1KLF FIMH ADHESIN-FIMC CHAPERONE COMPLEX WITH D-MANNOSE Deposited 2001-12-11 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain D
22–300（279 aa）
|
Not recorded | MAN alpha-D-mannopyranose × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.2；298 K；ammonium suplhate, pH 8.20, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 2.79 Å R-free 0.280 |
| 1KLF FIMH ADHESIN-FIMC CHAPERONE COMPLEX WITH D-MANNOSE Deposited 2001-12-11 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain F
22–300（279 aa）
|
Not recorded | MAN alpha-D-mannopyranose × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.2；298 K；ammonium suplhate, pH 8.20, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 2.79 Å R-free 0.280 |
| 1KLF FIMH ADHESIN-FIMC CHAPERONE COMPLEX WITH D-MANNOSE Deposited 2001-12-11 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain H
22–300（279 aa）
|
Not recorded | MAN alpha-D-mannopyranose × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.2；298 K；ammonium suplhate, pH 8.20, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 2.79 Å R-free 0.280 |
| 1KLF FIMH ADHESIN-FIMC CHAPERONE COMPLEX WITH D-MANNOSE Deposited 2001-12-11 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 5 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain J
22–300（279 aa）
|
Not recorded | MAN alpha-D-mannopyranose × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.2；298 K；ammonium suplhate, pH 8.20, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 2.79 Å R-free 0.280 |
| 1KLF FIMH ADHESIN-FIMC CHAPERONE COMPLEX WITH D-MANNOSE Deposited 2001-12-11 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 6 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain L
22–300（279 aa）
|
Not recorded | MAN alpha-D-mannopyranose × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.2；298 K；ammonium suplhate, pH 8.20, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 2.79 Å R-free 0.280 |
| 1KLF FIMH ADHESIN-FIMC CHAPERONE COMPLEX WITH D-MANNOSE Deposited 2001-12-11 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 7 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain N
22–300（279 aa）
|
Not recorded | MAN alpha-D-mannopyranose × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.2；298 K；ammonium suplhate, pH 8.20, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 2.79 Å R-free 0.280 |
| 1KLF FIMH ADHESIN-FIMC CHAPERONE COMPLEX WITH D-MANNOSE Deposited 2001-12-11 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 8 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain P
22–300（279 aa）
|
Not recorded | MAN alpha-D-mannopyranose × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.2；298 K；ammonium suplhate, pH 8.20, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 2.79 Å R-free 0.280 |
| 1QUN X-RAY STRUCTURE OF THE FIMC-FIMH CHAPERONE ADHESIN COMPLEX FROM UROPATHOGENIC E.COLI Deposited 1999-07-01 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
22–300（279 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.4；289 K；Ammonium Sulphate, Tris, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 289K
|
Resolution 2.80 Å R-free 0.268 |
| 1QUN X-RAY STRUCTURE OF THE FIMC-FIMH CHAPERONE ADHESIN COMPLEX FROM UROPATHOGENIC E.COLI Deposited 1999-07-01 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 10 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
22–300（279 aa）
Chain H
22–300（279 aa）
Chain L
22–300（279 aa）
Chain P
22–300（279 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.4；289 K；Ammonium Sulphate, Tris, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 289K
|
Resolution 2.80 Å R-free 0.268 |
| 1QUN X-RAY STRUCTURE OF THE FIMC-FIMH CHAPERONE ADHESIN COMPLEX FROM UROPATHOGENIC E.COLI Deposited 1999-07-01 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 11 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain D
22–300（279 aa）
Chain J
22–300（279 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.4；289 K；Ammonium Sulphate, Tris, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 289K
|
Resolution 2.80 Å R-free 0.268 |
| 1QUN X-RAY STRUCTURE OF THE FIMC-FIMH CHAPERONE ADHESIN COMPLEX FROM UROPATHOGENIC E.COLI Deposited 1999-07-01 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain D
22–300（279 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.4；289 K；Ammonium Sulphate, Tris, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 289K
|
Resolution 2.80 Å R-free 0.268 |
| 1QUN X-RAY STRUCTURE OF THE FIMC-FIMH CHAPERONE ADHESIN COMPLEX FROM UROPATHOGENIC E.COLI Deposited 1999-07-01 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain F
22–300（279 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.4；289 K；Ammonium Sulphate, Tris, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 289K
|
Resolution 2.80 Å R-free 0.268 |
| 1QUN X-RAY STRUCTURE OF THE FIMC-FIMH CHAPERONE ADHESIN COMPLEX FROM UROPATHOGENIC E.COLI Deposited 1999-07-01 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain H
22–300（279 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.4；289 K；Ammonium Sulphate, Tris, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 289K
|
Resolution 2.80 Å R-free 0.268 |
| 1QUN X-RAY STRUCTURE OF THE FIMC-FIMH CHAPERONE ADHESIN COMPLEX FROM UROPATHOGENIC E.COLI Deposited 1999-07-01 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 5 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain J
22–300（279 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.4；289 K；Ammonium Sulphate, Tris, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 289K
|
Resolution 2.80 Å R-free 0.268 |
| 1QUN X-RAY STRUCTURE OF THE FIMC-FIMH CHAPERONE ADHESIN COMPLEX FROM UROPATHOGENIC E.COLI Deposited 1999-07-01 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 6 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain L
22–300（279 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.4；289 K；Ammonium Sulphate, Tris, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 289K
|
Resolution 2.80 Å R-free 0.268 |
| 1QUN X-RAY STRUCTURE OF THE FIMC-FIMH CHAPERONE ADHESIN COMPLEX FROM UROPATHOGENIC E.COLI Deposited 1999-07-01 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 7 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain N
22–300（279 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.4；289 K；Ammonium Sulphate, Tris, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 289K
|
Resolution 2.80 Å R-free 0.268 |
| 1QUN X-RAY STRUCTURE OF THE FIMC-FIMH CHAPERONE ADHESIN COMPLEX FROM UROPATHOGENIC E.COLI Deposited 1999-07-01 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 8 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain P
22–300（279 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.4；289 K；Ammonium Sulphate, Tris, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 289K
|
Resolution 2.80 Å R-free 0.268 |
| 1QUN X-RAY STRUCTURE OF THE FIMC-FIMH CHAPERONE ADHESIN COMPLEX FROM UROPATHOGENIC E.COLI Deposited 1999-07-01 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 9 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain F
22–300（279 aa）
Chain N
22–300（279 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.4；289 K；Ammonium Sulphate, Tris, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 289K
|
Resolution 2.80 Å R-free 0.268 |
| 1TR7 FimH adhesin receptor binding domain from uropathogenic E. coli Deposited 2004-06-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
22–179（158 aa）
Fragment：N-terminal domain
|
Not recorded | DEG butyl alpha-D-mannopyranoside × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；291 K；MPD, cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K
|
Resolution 2.10 Å R-free 0.200 |
| 1TR7 FimH adhesin receptor binding domain from uropathogenic E. coli Deposited 2004-06-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
22–179（158 aa）
Fragment：N-terminal domain
|
Not recorded | DEG butyl alpha-D-mannopyranoside × 1 CAC CACODYLATE ION × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；291 K；MPD, cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K
|
Resolution 2.10 Å R-free 0.200 |
| 1UWF 1.7 A resolution structure of the receptor binding domain of the FimH adhesin from uropathogenic E. coli Deposited 2004-02-05 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
22–179（158 aa）
Fragment：N-TERMINAL LECTIN DOMAIN, RESIDUES 22-179
|
Not recorded | DEG butyl alpha-D-mannopyranoside × 1 GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7.5；1.1 MM PROTEIN, 0.1 M HEPES PH 7.5, 10 % PEG6000, 5% V/V MPD
|
Resolution 1.69 Å R-free 0.218 |
| 1ZE3 Crystal Structure of the Ternary Complex of FIMD (N-Terminal Domain) with FIMC and the Pilin Domain of FIMH Deposited 2005-04-17 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain H
179–300（122 aa）
Fragment：FimH pilin domain
|
Not recorded | EDO 1,2-ETHANEDIOL × 12 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；PEG 6000, MES buffer, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 1.84 Å R-free 0.217 |
| 2VCO Crystal structure of the fimbrial adhesin FimH in complex with its high-mannose epitope Deposited 2007-09-26 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
22–179（158 aa）
Fragment：RECEPTOR-BINDING DOMAIN, RESIDUES 22-179
Chain B
22–179（158 aa）
Fragment：RECEPTOR-BINDING DOMAIN, RESIDUES 22-179
|
Not recorded | NI NICKEL (II) ION × 4 SO4 SULFATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8.5；1.0 M LI2SO4, 0.1 M TRIS-HCL PH 8.5, 0.01 M NICL2, 3% GLYCEROL
|
Resolution 2.10 Å R-free 0.241 |
| 3ZL1 A thiazolyl-mannoside bound to FimH, monoclinic space group Deposited 2013-01-27 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
22–179（158 aa）
Fragment：LECTIN DOMAIN OR RECEPTOR BINDING DOMAIN, RESIDUES 22-179
|
Not recorded | BWG N-{5-[(1R)-1-hydroxyethyl]-1,3-thiazol-2-yl}-alpha-D-mannopyranosylamine × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7.5；0.1 M NACL, 0.1 M TRIS PH 7.5, 11 % (W/V) PEG 4000
|
Resolution 1.55 Å R-free 0.262 |
| 3ZL1 A thiazolyl-mannoside bound to FimH, monoclinic space group Deposited 2013-01-27 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
22–179（158 aa）
Fragment：LECTIN DOMAIN OR RECEPTOR BINDING DOMAIN, RESIDUES 22-179
|
Not recorded | BWG N-{5-[(1R)-1-hydroxyethyl]-1,3-thiazol-2-yl}-alpha-D-mannopyranosylamine × 1 CL CHLORIDE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7.5；0.1 M NACL, 0.1 M TRIS PH 7.5, 11 % (W/V) PEG 4000
|
Resolution 1.55 Å R-free 0.262 |
| 3ZL2 A thiazolyl-mannoside bound to FimH, orthorhombic space group Deposited 2013-01-27 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
22–179（158 aa）
Fragment：LECTIN DOMAIN OR RECEPTOR BINDING DOMAIN, RESIDUES 22-179
|
Not recorded | BWG N-{5-[(1R)-1-hydroxyethyl]-1,3-thiazol-2-yl}-alpha-D-mannopyranosylamine × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 6.5；20 MM NA/K PHOSPHATE, 0.1 M BIS TRIS PROPANE PH 6.5, 20 % (W/V) PEG 3350
|
Resolution 1.25 Å R-free 0.188 |
| 4CSS Crystal structure of FimH in complex with a sulfonamide biphenyl alpha D-mannoside Deposited 2014-03-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
22–180（159 aa）
Fragment：LECTIN DOMAIN, RESIDUES 22-180
|
Not recorded | CWX 4'-(alpha-D-Mannopyranosyloxy)-biphenyl-4-methyl sulfonamide × 1 GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 4.7；0.2 M SODIUM PHOSPHATE MONOBASIC MONOHYDRATE, 20% W/V PEG 3,350, pH 4.7
|
Resolution 1.07 Å R-free 0.132 |
| 4CST Crystal structure of FimH in complex with 3'-Chloro-4'-(alpha-D-mannopyranosyloxy)-biphenyl-4-carbonitrile Deposited 2014-03-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
22–180（159 aa）
Fragment：LECTIN DOMAIN, RESIDUES 22-180
|
Not recorded | CWK 3'-chloro-4'-(alpha-D-mannopyranosyloxy)biphenyl-4-carbonitrile × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
2.0 M AMMONIUM SULFATE, 0.1 M HEPES 7.5
|
Resolution 1.10 Å R-free 0.130 |
| 4J3O Crystal structure of the FimD usher traversed by the pilus tip complex assembly composed of FimC:FimF:FimG:FimH Deposited 2013-02-06 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric |
Chain H
22–300（279 aa）
Fragment：UNP residues 22-300
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；298 K；1.6-2.0M sodium formate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 3.80 Å R-free 0.299 |
| 4LOV Crystal structure of FimH in complex with Heptylmannoside Deposited 2013-07-13 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
22–179（158 aa）
Fragment：UNP residues 22-179
|
Not recorded | KGM heptyl alpha-D-mannopyranoside × 1 GOL GLYCEROL × 1 NA SODIUM ION × 3 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.50 Å R-free 0.190 |
| 4X50 Crystal structure of FimH in complex with biphenyl alpha-D-mannopyranoside Deposited 2014-12-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
22–180（159 aa）
|
Not recorded | 3X8 biphenyl-4-yl alpha-D-mannopyranoside × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；19% PEG 8000, 0.2 M (NH4)2SO4, 0.1 M HEPES pH 7.0.
|
Resolution 2.00 Å R-free 0.202 |
| 4X50 Crystal structure of FimH in complex with biphenyl alpha-D-mannopyranoside Deposited 2014-12-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
22–180（159 aa）
|
Not recorded | 3X8 biphenyl-4-yl alpha-D-mannopyranoside × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；19% PEG 8000, 0.2 M (NH4)2SO4, 0.1 M HEPES pH 7.0.
|
Resolution 2.00 Å R-free 0.202 |
| 4X5P Crystal structure of FimH in complex with a benzoyl-amidophenyl alpha-D-mannopyranoside Deposited 2014-12-05 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
22–180（159 aa）
Fragment：UNP residues 22-180
|
Not recorded | 3XJ 4-{[3-chloro-4-(alpha-D-mannopyranosyloxy)phenyl]carbamoyl}benzoic acid × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 4.7；293 K；20% PEG4000, 0.2 M NaH2PO4, pH 4.7
|
Resolution 1.00 Å R-free 0.139 |
| 4X5Q Crystal structure of FimH in complex with 5-nitro-indolinylphenyl alpha-D-mannopyranoside Deposited 2014-12-05 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
22–180（159 aa）
Fragment：UNP residues 22-180
|
Not recorded | 3XN 4-(5-nitro-1H-indol-1-yl)phenyl alpha-D-mannopyranoside × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 4.7；293 K；20% PEG 3,350, 0.2 M sodium phosphate monobasic monohydrate
|
Resolution 1.12 Å R-free 0.129 |
| 4X5R Crystal structure of FimH in complex with a squaryl-phenyl alpha-D-mannopyranoside derivative Deposited 2014-12-05 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: Monomeric |
Chain A
22–180（159 aa）
Fragment：UNP residues 22-180
|
Not recorded | 3XO 2-chloro-4-{[2-(4-methylpiperazin-1-yl)-3,4-dioxocyclobut-1-en-1-yl]amino}phenyl alpha-D-mannopyranoside × 1 SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；277 K；1.5 M Ammonium sulfate, 0.1 M HEPES pH 7.0
|
Resolution 1.65 Å R-free 0.204 |
| 4X5R Crystal structure of FimH in complex with a squaryl-phenyl alpha-D-mannopyranoside derivative Deposited 2014-12-05 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: Monomeric |
Chain B
22–180（159 aa）
Fragment：UNP residues 22-180
|
Not recorded | 3XO 2-chloro-4-{[2-(4-methylpiperazin-1-yl)-3,4-dioxocyclobut-1-en-1-yl]amino}phenyl alpha-D-mannopyranoside × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；277 K；1.5 M Ammonium sulfate, 0.1 M HEPES pH 7.0
|
Resolution 1.65 Å R-free 0.204 |
| 4X5R Crystal structure of FimH in complex with a squaryl-phenyl alpha-D-mannopyranoside derivative Deposited 2014-12-05 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: Monomeric |
Chain C
22–180（159 aa）
Fragment：UNP residues 22-180
|
Not recorded | 3XO 2-chloro-4-{[2-(4-methylpiperazin-1-yl)-3,4-dioxocyclobut-1-en-1-yl]amino}phenyl alpha-D-mannopyranoside × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；277 K；1.5 M Ammonium sulfate, 0.1 M HEPES pH 7.0
|
Resolution 1.65 Å R-free 0.204 |
| 4XO8 Crystal structure of the FimH lectin domain from E.coli K12 in complex with heptyl alpha-D-mannopyrannoside Deposited 2015-01-16 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
22–179（158 aa）
Fragment：lectin domain, UNP residues 22-179
|
Not recorded | KGM heptyl alpha-D-mannopyranoside × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.5；293 K；1.5 M (NH4)2SO4, 0.2 M Na Acetate pH 5.5
|
Resolution 1.70 Å R-free 0.195 |
| 4XO8 Crystal structure of the FimH lectin domain from E.coli K12 in complex with heptyl alpha-D-mannopyrannoside Deposited 2015-01-16 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
22–179（158 aa）
Fragment：lectin domain, UNP residues 22-179
|
Not recorded | KGM heptyl alpha-D-mannopyranoside × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.5；293 K；1.5 M (NH4)2SO4, 0.2 M Na Acetate pH 5.5
|
Resolution 1.70 Å R-free 0.195 |
| 4XO9 Crystal structure of a FimH*DsG complex from E.coli K12 in space group C2 Deposited 2015-01-16 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
22–300（279 aa）
Fragment：UNP residues 22-300
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.5；278 K；25 % (w/v) polyethylene glycol (PEG) 3350, 0.2 M magnesium chloride, 0.1 M BisTris-HCl pH 5.5
|
Resolution 1.14 Å R-free 0.143 |
| 4XOA Crystal structure of a FimH*DsG complex from E.coli K12 in space group P1 Deposited 2015-01-16 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
22–300（279 aa）
Fragment：UNP residues 22-300
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5；293 K；0.2M Na Malonate, 20%PEG3350
|
Resolution 2.54 Å R-free 0.260 |
| 4XOA Crystal structure of a FimH*DsG complex from E.coli K12 in space group P1 Deposited 2015-01-16 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain G
22–300（279 aa）
Fragment：UNP residues 22-300
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5；293 K；0.2M Na Malonate, 20%PEG3350
|
Resolution 2.54 Å R-free 0.260 |
| 4XOA Crystal structure of a FimH*DsG complex from E.coli K12 in space group P1 Deposited 2015-01-16 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
22–300（279 aa）
Fragment：UNP residues 22-300
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5；293 K；0.2M Na Malonate, 20%PEG3350
|
Resolution 2.54 Å R-free 0.260 |
| 4XOA Crystal structure of a FimH*DsG complex from E.coli K12 in space group P1 Deposited 2015-01-16 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain E
22–300（279 aa）
Fragment：UNP residues 22-300
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5；293 K；0.2M Na Malonate, 20%PEG3350
|
Resolution 2.54 Å R-free 0.260 |
| 4XOB Crystal structure of a FimH*DsF complex from E.coli K12 with bound heptyl alpha-D-mannopyrannoside Deposited 2015-01-16 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
22–300（279 aa）
Fragment：UNP residues 22-300
|
Not recorded | KGM heptyl alpha-D-mannopyranoside × 1 SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；30 % w/v PEG 5,000, 0.1 M MES monohydrate, 0.2 M Ammonium sulfate pH 6.5. 2.5fold excess ligand to protein
|
Resolution 3.00 Å R-free 0.245 |
| 4XOB Crystal structure of a FimH*DsF complex from E.coli K12 with bound heptyl alpha-D-mannopyrannoside Deposited 2015-01-16 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
22–300（279 aa）
Fragment：UNP residues 22-300
|
Not recorded | KGM heptyl alpha-D-mannopyranoside × 1 SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；30 % w/v PEG 5,000, 0.1 M MES monohydrate, 0.2 M Ammonium sulfate pH 6.5. 2.5fold excess ligand to protein
|
Resolution 3.00 Å R-free 0.245 |
| 4XOB Crystal structure of a FimH*DsF complex from E.coli K12 with bound heptyl alpha-D-mannopyrannoside Deposited 2015-01-16 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain E
22–300（279 aa）
Fragment：UNP residues 22-300
|
Not recorded | KGM heptyl alpha-D-mannopyranoside × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；30 % w/v PEG 5,000, 0.1 M MES monohydrate, 0.2 M Ammonium sulfate pH 6.5. 2.5fold excess ligand to protein
|
Resolution 3.00 Å R-free 0.245 |
| 4XOB Crystal structure of a FimH*DsF complex from E.coli K12 with bound heptyl alpha-D-mannopyrannoside Deposited 2015-01-16 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain G
22–300（279 aa）
Fragment：UNP residues 22-300
|
Not recorded | KGM heptyl alpha-D-mannopyranoside × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；30 % w/v PEG 5,000, 0.1 M MES monohydrate, 0.2 M Ammonium sulfate pH 6.5. 2.5fold excess ligand to protein
|
Resolution 3.00 Å R-free 0.245 |
| 5ABZ Complex of the FimH lectin with a C-linked naphtyl alpha-D-mannoside in soaked trigonal crystals at 2.40 A resolution Deposited 2015-08-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
22–179（158 aa）
Fragment：LECTIN DOMAIN, UNP RESIDUES 22-179
|
Not recorded | Z47 (2R,3S,4R,5S,6R)-2-(HYDROXYMETHYL)-6-[3-(NAPHTHALEN-1-YL)PROPYL]OXANE-3,4,5-TRIOL × 1 NI NICKEL (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8.6；PH 8.6
|
Resolution 2.40 Å R-free 0.206 |
| 5ABZ Complex of the FimH lectin with a C-linked naphtyl alpha-D-mannoside in soaked trigonal crystals at 2.40 A resolution Deposited 2015-08-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
22–179（158 aa）
Fragment：LECTIN DOMAIN, UNP RESIDUES 22-179
|
Not recorded | Z47 (2R,3S,4R,5S,6R)-2-(HYDROXYMETHYL)-6-[3-(NAPHTHALEN-1-YL)PROPYL]OXANE-3,4,5-TRIOL × 1 NI NICKEL (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8.6；PH 8.6
|
Resolution 2.40 Å R-free 0.206 |
| 5CGB Crystal structure of FimH in complex with heptyl alpha-D-septanoside Deposited 2015-07-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
22–179（158 aa）
|
Not recorded | 51C (6R)-1,6-anhydro-2-O-heptyl-6-(hydroxymethyl)-D-galactitol × 1 SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.5；277 K；1.5 M NH4SO4, 0.1 M BisTris pH 5.5
|
Resolution 1.60 Å R-free 0.179 |
| 5CGB Crystal structure of FimH in complex with heptyl alpha-D-septanoside Deposited 2015-07-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
22–179（158 aa）
|
Not recorded | 51C (6R)-1,6-anhydro-2-O-heptyl-6-(hydroxymethyl)-D-galactitol × 1 SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.5；277 K；1.5 M NH4SO4, 0.1 M BisTris pH 5.5
|
Resolution 1.60 Å R-free 0.179 |
| 5F2F Crystal structure of para-biphenyl-2-methyl-3', 5' di-methyl amide mannoside bound to FimH lectin domain Deposited 2015-12-01 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
22–179（158 aa）
Fragment：lectin domain (UNP residues 22-179)
|
Not recorded | 5U7 5-[4-[(2~{R},3~{S},4~{S},5~{S},6~{R})-6-(hydroxymethyl)-3,4,5-tris(oxidanyl)oxan-2-yl]oxy-3-methyl-phenyl]-~{N}1,~{N}3-dimethyl-benzene-1,3-dicarboxamide × 1 GOL GLYCEROL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.8；291 K；0.2 M AmSO4, 20% PEG3350
|
Resolution 1.67 Å R-free 0.177 |
| 5F3F Crystal structure of para-biphenyl-2-methyl-3'-methyl amide mannoside bound to FimH lectin domain Deposited 2015-12-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
22–179（158 aa）
Fragment：lectin domain (UNP residues 22-179)
|
Not recorded | 5US 3-[4-[(2~{R},3~{S},4~{S},5~{S},6~{R})-6-(hydroxymethyl)-3,4,5-tris(oxidanyl)oxan-2-yl]oxy-3-methyl-phenyl]-~{N}-methyl-benzamide × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.8；291 K；0.2 M AmSO4, 20% PEG 3350
|
Resolution 1.76 Å R-free 0.172 |
| 5JCQ Crystal structure of the FimH lectin domain from E.coli K12 in complex with methyl alpha-D-mannopyrannoside in spacegroup P21 Deposited 2016-04-15 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
22–179（158 aa）
|
Not recorded | MMA methyl alpha-D-mannopyranoside × 1 SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；0.2 M Ammoniumsulfat, 30% PEG3350, 0.1 M Hepes 7.0, frozen by addition of 20 % EG
|
Resolution 1.60 Å R-free 0.214 |
| 5JCQ Crystal structure of the FimH lectin domain from E.coli K12 in complex with methyl alpha-D-mannopyrannoside in spacegroup P21 Deposited 2016-04-15 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
22–179（158 aa）
|
Not recorded | MMA methyl alpha-D-mannopyranoside × 1 SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；0.2 M Ammoniumsulfat, 30% PEG3350, 0.1 M Hepes 7.0, frozen by addition of 20 % EG
|
Resolution 1.60 Å R-free 0.214 |
| 5JCR Crystal structure of the FimH lectin domain from E.coli K12 in complex with methyl alpha-D-mannopyrannoside in spacegroup P212121 Deposited 2016-04-15 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
22–179（158 aa）
|
Not recorded | MMA methyl alpha-D-mannopyranoside × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；0.2 M Ammoniumsulfat, 30% PEG3350, 0.1 M Hepes 7.0, frozen by addition of 20 % EG
|
Resolution 1.70 Å R-free 0.230 |
| 5JCR Crystal structure of the FimH lectin domain from E.coli K12 in complex with methyl alpha-D-mannopyrannoside in spacegroup P212121 Deposited 2016-04-15 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
22–179（158 aa）
|
Not recorded | MMA methyl alpha-D-mannopyranoside × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；0.2 M Ammoniumsulfat, 30% PEG3350, 0.1 M Hepes 7.0, frozen by addition of 20 % EG
|
Resolution 1.70 Å R-free 0.230 |
| 5L4T Crystal structure of FimH lectin domain in complex with 2-Deoxy-Heptylmannoside Deposited 2016-05-26 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
22–179（158 aa）
|
Not recorded | 6LS heptyl 2-deoxy-alpha-D-mannopyranoside × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；285 K；0.2 M (NH4)2SO4, 0.1 M Hepes pH 7 and 25-30% PEG3350
|
Resolution 1.90 Å R-free 0.218 |
| 5L4T Crystal structure of FimH lectin domain in complex with 2-Deoxy-Heptylmannoside Deposited 2016-05-26 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
22–179（158 aa）
|
Not recorded | 6LS heptyl 2-deoxy-alpha-D-mannopyranoside × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；285 K；0.2 M (NH4)2SO4, 0.1 M Hepes pH 7 and 25-30% PEG3350
|
Resolution 1.90 Å R-free 0.218 |
| 5L4U Crystal structure of FimH lectin domain in complex with 2-Fluoro-Heptylmannoside Deposited 2016-05-26 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
22–179（158 aa）
|
Not recorded | 6KS heptyl 2-fluoro-alpha-D-mannopyranoside × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；285 K；0.2 M (NH4)2SO4, 0.1 M Hepes pH 7 and 25-30% PEG3350
|
Resolution 2.10 Å R-free 0.236 |
| 5L4U Crystal structure of FimH lectin domain in complex with 2-Fluoro-Heptylmannoside Deposited 2016-05-26 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
22–179（158 aa）
|
Not recorded | 6KS heptyl 2-fluoro-alpha-D-mannopyranoside × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；285 K；0.2 M (NH4)2SO4, 0.1 M Hepes pH 7 and 25-30% PEG3350
|
Resolution 2.10 Å R-free 0.236 |
| 5L4V Crystal structure of FimH lectin domain in complex with 3-Deoxy-Heptylmannoside Deposited 2016-05-26 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
22–179（158 aa）
|
Not recorded | 6KW heptyl 3-deoxy-alpha-D-mannopyranoside × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；285 K；0.2 M (NH4)2SO4, 0.1 M Hepes pH 7 and 25-30% PEG3350
|
Resolution 2.99 Å R-free 0.252 |
| 5L4V Crystal structure of FimH lectin domain in complex with 3-Deoxy-Heptylmannoside Deposited 2016-05-26 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
22–179（158 aa）
|
Not recorded | 6KW heptyl 3-deoxy-alpha-D-mannopyranoside × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；285 K；0.2 M (NH4)2SO4, 0.1 M Hepes pH 7 and 25-30% PEG3350
|
Resolution 2.99 Å R-free 0.252 |
| 5L4V Crystal structure of FimH lectin domain in complex with 3-Deoxy-Heptylmannoside Deposited 2016-05-26 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
22–179（158 aa）
|
Not recorded | 6KW heptyl 3-deoxy-alpha-D-mannopyranoside × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；285 K；0.2 M (NH4)2SO4, 0.1 M Hepes pH 7 and 25-30% PEG3350
|
Resolution 2.99 Å R-free 0.252 |
| 5L4W Crystal structure of FimH lectin domain in complex with 3-Fluoro-Heptylmannoside Deposited 2016-05-26 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
22–179（158 aa）
|
Not recorded | 6KH heptyl 3-fluoro-alpha-D-mannopyranoside × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；285 K；0.2 M (NH4)2SO4, 0.1 M Hepes pH 7 and 25-30% PEG3350
|
Resolution 1.90 Å R-free 0.202 |
| 5L4W Crystal structure of FimH lectin domain in complex with 3-Fluoro-Heptylmannoside Deposited 2016-05-26 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
22–179（158 aa）
|
Not recorded | 6KH heptyl 3-fluoro-alpha-D-mannopyranoside × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；285 K；0.2 M (NH4)2SO4, 0.1 M Hepes pH 7 and 25-30% PEG3350
|
Resolution 1.90 Å R-free 0.202 |
| 5L4W Crystal structure of FimH lectin domain in complex with 3-Fluoro-Heptylmannoside Deposited 2016-05-26 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
22–179（158 aa）
|
Not recorded | 6KH heptyl 3-fluoro-alpha-D-mannopyranoside × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；285 K；0.2 M (NH4)2SO4, 0.1 M Hepes pH 7 and 25-30% PEG3350
|
Resolution 1.90 Å R-free 0.202 |
| 5L4X Crystal structure of FimH lectin domain in complex with 4-Deoxy-Heptylmannoside Deposited 2016-05-26 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
22–179（158 aa）
|
Not recorded | 6KU heptyl 4-deoxy-4-deoxy-alpha-D-mannopyranoside × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；285 K；0.2 M (NH4)2SO4, 0.1 M Hepes pH 7 and 25-30% PEG3350
|
Resolution 1.90 Å R-free 0.204 |
| 5L4X Crystal structure of FimH lectin domain in complex with 4-Deoxy-Heptylmannoside Deposited 2016-05-26 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
22–179（158 aa）
|
Not recorded | 6KU heptyl 4-deoxy-4-deoxy-alpha-D-mannopyranoside × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；285 K；0.2 M (NH4)2SO4, 0.1 M Hepes pH 7 and 25-30% PEG3350
|
Resolution 1.90 Å R-free 0.204 |
| 5L4Y Crystal structure of FimH lectin domain in complex with 4-Fluoro-Heptylmannoside Deposited 2016-05-26 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
22–179（158 aa）
|
Not recorded | 6K3 heptyl 4-deoxy-4-fluoro-alpha-D-mannopyranoside × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；285 K；0.2 M (NH4)2SO4, 0.1 M Hepes pH 7 and 25-30% PEG3350
|
Resolution 1.90 Å R-free 0.229 |
| 5L4Y Crystal structure of FimH lectin domain in complex with 4-Fluoro-Heptylmannoside Deposited 2016-05-26 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
22–179（158 aa）
|
Not recorded | 6K3 heptyl 4-deoxy-4-fluoro-alpha-D-mannopyranoside × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；285 K；0.2 M (NH4)2SO4, 0.1 M Hepes pH 7 and 25-30% PEG3350
|
Resolution 1.90 Å R-free 0.229 |
| 5MCA Crystal structure of FimH-LD R60P variant in the apo state Deposited 2016-11-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
22–180（159 aa）
|
Mutation：R60P | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；1.5 M (NH4)2SO4, 0.1M BisTris 6.5
|
Resolution 1.60 Å R-free 0.175 |
| 5MTS Complex of FimH lectin with a TazMan (thiazolylaminomannosides) family member known as potent anti-adhesive agent at 2.6 A resolution Deposited 2017-01-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–300（300 aa）
|
Not recorded | XKA [2-[[(2~{S},3~{S},4~{S},5~{S},6~{R})-6-(hydroxymethyl)-3,4,5-tris(oxidanyl)oxan-2-yl]amino]-1,3-thiazol-5-yl]-(4-methyl-2-pyrazin-2-yl-1,3-thiazol-5-yl)methanone × 1 NI NICKEL (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；300 K；1.0 M LI2SO4
0.1 M TRIS-HCL
0.01 M NICL2
3% GLYCEROL
|
Resolution 2.60 Å R-free 0.250 |
| 5MTS Complex of FimH lectin with a TazMan (thiazolylaminomannosides) family member known as potent anti-adhesive agent at 2.6 A resolution Deposited 2017-01-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
1–300（300 aa）
|
Not recorded | XKA [2-[[(2~{S},3~{S},4~{S},5~{S},6~{R})-6-(hydroxymethyl)-3,4,5-tris(oxidanyl)oxan-2-yl]amino]-1,3-thiazol-5-yl]-(4-methyl-2-pyrazin-2-yl-1,3-thiazol-5-yl)methanone × 1 NI NICKEL (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；300 K；1.0 M LI2SO4
0.1 M TRIS-HCL
0.01 M NICL2
3% GLYCEROL
|
Resolution 2.60 Å R-free 0.250 |
| 5MUC Crystal structure of the FimH lectin domain in complex with 1,5-Anhydromannitol Deposited 2017-01-13 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
22–179（158 aa）
|
Not recorded | AH2 1-deoxy-alpha-D-mannopyranose × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；1.6 M Ammoniumsulfat, 0.1 M Hepes 7.5, 1% PEG3350
|
Resolution 2.60 Å R-free 0.260 |
| 5MUC Crystal structure of the FimH lectin domain in complex with 1,5-Anhydromannitol Deposited 2017-01-13 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
22–179（158 aa）
|
Not recorded | AH2 1-deoxy-alpha-D-mannopyranose × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；1.6 M Ammoniumsulfat, 0.1 M Hepes 7.5, 1% PEG3350
|
Resolution 2.60 Å R-free 0.260 |
| 6E14 Handover mechanism of the growing pilus by the bacterial outer membrane usher FimD Deposited 2018-07-09 | Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric |
Chain H
1–300（300 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.00 Å |
| 6E15 Handover mechanism of the growing pilus by the bacterial outer membrane usher FimD Deposited 2018-07-09 | Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric |
Chain H
1–300（300 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.10 Å |
| 6G2R Crystal structure of FimH in complex with a tetraflourinated biphenyl alpha D-mannoside Deposited 2018-03-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
22–179（158 aa）
|
Not recorded | EJK 4-[3-chloranyl-4-[(2~{R},3~{S},4~{S},5~{S},6~{R})-6-(hydroxymethyl)-3,4,5-tris(oxidanyl)oxan-2-yl]oxy-phenyl]-2,3,5,6-tetrakis(fluoranyl)benzenecarbonitrile × 1 SO4 SULFATE ION × 1 EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；0.1 M NaAcetate pH 5.0, 1.5 M NH4sulfate
|
Resolution 2.10 Å R-free 0.222 |
| 6G2R Crystal structure of FimH in complex with a tetraflourinated biphenyl alpha D-mannoside Deposited 2018-03-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
22–179（158 aa）
|
Not recorded | EJK 4-[3-chloranyl-4-[(2~{R},3~{S},4~{S},5~{S},6~{R})-6-(hydroxymethyl)-3,4,5-tris(oxidanyl)oxan-2-yl]oxy-phenyl]-2,3,5,6-tetrakis(fluoranyl)benzenecarbonitrile × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；0.1 M NaAcetate pH 5.0, 1.5 M NH4sulfate
|
Resolution 2.10 Å R-free 0.222 |
| 6G2S Crystal structure of FimH in complex with a pentaflourinated biphenyl alpha D-mannoside Deposited 2018-03-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
22–179（158 aa）
|
Not recorded | EJN (2~{R},3~{S},4~{S},5~{S},6~{R})-2-(hydroxymethyl)-6-[4-[2,3,4,5,6-pentakis(fluoranyl)phenyl]phenoxy]oxane-3,4,5-triol × 1 SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；0.1 M BisTrisPropane, propionic acid, cacodylate (PCPT buffer) pH 8.0, 0.1 M (NH4)2SO4 and 25 % PEG1500
|
Resolution 2.20 Å R-free 0.222 |
| 6G2S Crystal structure of FimH in complex with a pentaflourinated biphenyl alpha D-mannoside Deposited 2018-03-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
22–179（158 aa）
|
Not recorded | EJN (2~{R},3~{S},4~{S},5~{S},6~{R})-2-(hydroxymethyl)-6-[4-[2,3,4,5,6-pentakis(fluoranyl)phenyl]phenoxy]oxane-3,4,5-triol × 1 SO4 SULFATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；0.1 M BisTrisPropane, propionic acid, cacodylate (PCPT buffer) pH 8.0, 0.1 M (NH4)2SO4 and 25 % PEG1500
|
Resolution 2.20 Å R-free 0.222 |
| 6G2S Crystal structure of FimH in complex with a pentaflourinated biphenyl alpha D-mannoside Deposited 2018-03-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
22–179（158 aa）
|
Not recorded | EJN (2~{R},3~{S},4~{S},5~{S},6~{R})-2-(hydroxymethyl)-6-[4-[2,3,4,5,6-pentakis(fluoranyl)phenyl]phenoxy]oxane-3,4,5-triol × 1 SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；0.1 M BisTrisPropane, propionic acid, cacodylate (PCPT buffer) pH 8.0, 0.1 M (NH4)2SO4 and 25 % PEG1500
|
Resolution 2.20 Å R-free 0.222 |
| 6G2S Crystal structure of FimH in complex with a pentaflourinated biphenyl alpha D-mannoside Deposited 2018-03-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
22–179（158 aa）
|
Not recorded | EJN (2~{R},3~{S},4~{S},5~{S},6~{R})-2-(hydroxymethyl)-6-[4-[2,3,4,5,6-pentakis(fluoranyl)phenyl]phenoxy]oxane-3,4,5-triol × 1 SO4 SULFATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；0.1 M BisTrisPropane, propionic acid, cacodylate (PCPT buffer) pH 8.0, 0.1 M (NH4)2SO4 and 25 % PEG1500
|
Resolution 2.20 Å R-free 0.222 |
| 6G2S Crystal structure of FimH in complex with a pentaflourinated biphenyl alpha D-mannoside Deposited 2018-03-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 5 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain E
22–179（158 aa）
|
Not recorded | EJN (2~{R},3~{S},4~{S},5~{S},6~{R})-2-(hydroxymethyl)-6-[4-[2,3,4,5,6-pentakis(fluoranyl)phenyl]phenoxy]oxane-3,4,5-triol × 1 SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；0.1 M BisTrisPropane, propionic acid, cacodylate (PCPT buffer) pH 8.0, 0.1 M (NH4)2SO4 and 25 % PEG1500
|
Resolution 2.20 Å R-free 0.222 |
| 6G2S Crystal structure of FimH in complex with a pentaflourinated biphenyl alpha D-mannoside Deposited 2018-03-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 6 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain F
22–179（158 aa）
|
Not recorded | EJN (2~{R},3~{S},4~{S},5~{S},6~{R})-2-(hydroxymethyl)-6-[4-[2,3,4,5,6-pentakis(fluoranyl)phenyl]phenoxy]oxane-3,4,5-triol × 1 SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；0.1 M BisTrisPropane, propionic acid, cacodylate (PCPT buffer) pH 8.0, 0.1 M (NH4)2SO4 and 25 % PEG1500
|
Resolution 2.20 Å R-free 0.222 |
| 6G2S Crystal structure of FimH in complex with a pentaflourinated biphenyl alpha D-mannoside Deposited 2018-03-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 7 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain G
22–179（158 aa）
|
Not recorded | EJN (2~{R},3~{S},4~{S},5~{S},6~{R})-2-(hydroxymethyl)-6-[4-[2,3,4,5,6-pentakis(fluoranyl)phenyl]phenoxy]oxane-3,4,5-triol × 1 SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；0.1 M BisTrisPropane, propionic acid, cacodylate (PCPT buffer) pH 8.0, 0.1 M (NH4)2SO4 and 25 % PEG1500
|
Resolution 2.20 Å R-free 0.222 |
| 6G2S Crystal structure of FimH in complex with a pentaflourinated biphenyl alpha D-mannoside Deposited 2018-03-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 8 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain H
22–179（158 aa）
|
Not recorded | EJN (2~{R},3~{S},4~{S},5~{S},6~{R})-2-(hydroxymethyl)-6-[4-[2,3,4,5,6-pentakis(fluoranyl)phenyl]phenoxy]oxane-3,4,5-triol × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；0.1 M BisTrisPropane, propionic acid, cacodylate (PCPT buffer) pH 8.0, 0.1 M (NH4)2SO4 and 25 % PEG1500
|
Resolution 2.20 Å R-free 0.222 |
| 6G2S Crystal structure of FimH in complex with a pentaflourinated biphenyl alpha D-mannoside Deposited 2018-03-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 9 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain I
22–179（158 aa）
|
Not recorded | EJN (2~{R},3~{S},4~{S},5~{S},6~{R})-2-(hydroxymethyl)-6-[4-[2,3,4,5,6-pentakis(fluoranyl)phenyl]phenoxy]oxane-3,4,5-triol × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；0.1 M BisTrisPropane, propionic acid, cacodylate (PCPT buffer) pH 8.0, 0.1 M (NH4)2SO4 and 25 % PEG1500
|
Resolution 2.20 Å R-free 0.222 |
| 6GTX Crystal structure of the FimH lectin domain from E.coli K12 in complex with the dimannoside Man(alpha1-2)Man Deposited 2018-06-19 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
22–179（158 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；1.5 M (NH4)2SO4, 0.1 M BisTris-HCl pH 5.5
|
Resolution 2.50 Å R-free 0.240 |
| 6GTX Crystal structure of the FimH lectin domain from E.coli K12 in complex with the dimannoside Man(alpha1-2)Man Deposited 2018-06-19 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 2 Other combination Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
22–179（158 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；1.5 M (NH4)2SO4, 0.1 M BisTris-HCl pH 5.5
|
Resolution 2.50 Å R-free 0.240 |
| 6GTX Crystal structure of the FimH lectin domain from E.coli K12 in complex with the dimannoside Man(alpha1-2)Man Deposited 2018-06-19 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Other combination Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
22–179（158 aa）
|
Not recorded | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；1.5 M (NH4)2SO4, 0.1 M BisTris-HCl pH 5.5
|
Resolution 2.50 Å R-free 0.240 |
| 6GTX Crystal structure of the FimH lectin domain from E.coli K12 in complex with the dimannoside Man(alpha1-2)Man Deposited 2018-06-19 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Other combination Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
22–179（158 aa）
|
Not recorded | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；1.5 M (NH4)2SO4, 0.1 M BisTris-HCl pH 5.5
|
Resolution 2.50 Å R-free 0.240 |
| 6GTY Crystal structure of the FimH lectin domain from E.coli K12 in complex with the dimannoside Man(alpha1-6)Man Deposited 2018-06-19 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
22–179（158 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；0.1 M ammonium sulfate, 0.3 M sodium formate, 0.1 M Tris pH 7.8, 3% w/v PGA-LM, 30% w/v PGA-LM PEG20K
|
Resolution 1.90 Å R-free 0.216 |
| 6GTY Crystal structure of the FimH lectin domain from E.coli K12 in complex with the dimannoside Man(alpha1-6)Man Deposited 2018-06-19 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 2 Other combination Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
22–179（158 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；0.1 M ammonium sulfate, 0.3 M sodium formate, 0.1 M Tris pH 7.8, 3% w/v PGA-LM, 30% w/v PGA-LM PEG20K
|
Resolution 1.90 Å R-free 0.216 |
| 6GTY Crystal structure of the FimH lectin domain from E.coli K12 in complex with the dimannoside Man(alpha1-6)Man Deposited 2018-06-19 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 3 Other combination Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
22–179（158 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；0.1 M ammonium sulfate, 0.3 M sodium formate, 0.1 M Tris pH 7.8, 3% w/v PGA-LM, 30% w/v PGA-LM PEG20K
|
Resolution 1.90 Å R-free 0.216 |
| 6GTY Crystal structure of the FimH lectin domain from E.coli K12 in complex with the dimannoside Man(alpha1-6)Man Deposited 2018-06-19 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 4 Other combination Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
22–179（158 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；0.1 M ammonium sulfate, 0.3 M sodium formate, 0.1 M Tris pH 7.8, 3% w/v PGA-LM, 30% w/v PGA-LM PEG20K
|
Resolution 1.90 Å R-free 0.216 |
| 6GTY Crystal structure of the FimH lectin domain from E.coli K12 in complex with the dimannoside Man(alpha1-6)Man Deposited 2018-06-19 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 5 Other combination Monomer；Protein × 1 PDB declaration: monomeric |
Chain E
22–179（158 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；0.1 M ammonium sulfate, 0.3 M sodium formate, 0.1 M Tris pH 7.8, 3% w/v PGA-LM, 30% w/v PGA-LM PEG20K
|
Resolution 1.90 Å R-free 0.216 |
| 7AYN Crystal structure of the lectin domain of the FimH variant Arg98Ala, in complex with Methyl 3-chloro-4-D-mannopyranosyloxy-3-biphenylcarboxylate Deposited 2020-11-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
22–179（158 aa）
|
Not recorded | SBQ methyl 3-[3-chloranyl-4-[(2~{R},3~{S},4~{S},5~{S},6~{R})-6-(hydroxymethyl)-3,4,5-tris(oxidanyl)oxan-2-yl]oxy-phenyl]benzoate × 1 EDO 1,2-ETHANEDIOL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；6% Tacsimate pH 6.0, 12% PEG3350
|
Resolution 1.42 Å R-free 0.184 |
| 7AYN Crystal structure of the lectin domain of the FimH variant Arg98Ala, in complex with Methyl 3-chloro-4-D-mannopyranosyloxy-3-biphenylcarboxylate Deposited 2020-11-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
22–179（158 aa）
|
Not recorded | SBQ methyl 3-[3-chloranyl-4-[(2~{R},3~{S},4~{S},5~{S},6~{R})-6-(hydroxymethyl)-3,4,5-tris(oxidanyl)oxan-2-yl]oxy-phenyl]benzoate × 1 EDO 1,2-ETHANEDIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；6% Tacsimate pH 6.0, 12% PEG3350
|
Resolution 1.42 Å R-free 0.184 |
| 7AYN Crystal structure of the lectin domain of the FimH variant Arg98Ala, in complex with Methyl 3-chloro-4-D-mannopyranosyloxy-3-biphenylcarboxylate Deposited 2020-11-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
22–179（158 aa）
|
Not recorded | SBQ methyl 3-[3-chloranyl-4-[(2~{R},3~{S},4~{S},5~{S},6~{R})-6-(hydroxymethyl)-3,4,5-tris(oxidanyl)oxan-2-yl]oxy-phenyl]benzoate × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；6% Tacsimate pH 6.0, 12% PEG3350
|
Resolution 1.42 Å R-free 0.184 |
| 7AYN Crystal structure of the lectin domain of the FimH variant Arg98Ala, in complex with Methyl 3-chloro-4-D-mannopyranosyloxy-3-biphenylcarboxylate Deposited 2020-11-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
22–179（158 aa）
|
Not recorded | SBQ methyl 3-[3-chloranyl-4-[(2~{R},3~{S},4~{S},5~{S},6~{R})-6-(hydroxymethyl)-3,4,5-tris(oxidanyl)oxan-2-yl]oxy-phenyl]benzoate × 1 EDO 1,2-ETHANEDIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；6% Tacsimate pH 6.0, 12% PEG3350
|
Resolution 1.42 Å R-free 0.184 |
| 7BHD FimH in complex with alpha1,6 core-fucosylated oligomannose-3, crystallized in the trigonal space group Deposited 2021-01-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
22–179（158 aa）
Chain B
22–179（158 aa）
|
Not recorded | NI NICKEL (II) ION × 2 SO4 SULFATE ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 9；291 K；1,1M Lithium sulfate, 0,1M Tris-HCl pH=9, 0,01M Nickel Chloride
|
Resolution 1.40 Å R-free 0.198 |
| 8BVD FimH lectin domain in complex with mannose C-linked to quinoline Deposited 2022-12-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
22–179（158 aa）
|
Not recorded | RLO (2R,3S,4R,5S,6R)-2-(hydroxymethyl)-6-[(E)-3-quinolin-6-ylprop-2-enyl]oxane-3,4,5-triol × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.5；293.15 K；3.0M NaCl and 0.1M BIS-TRIS at pH=5.5
|
Resolution 3.00 Å R-free 0.303 |
| 8BVD FimH lectin domain in complex with mannose C-linked to quinoline Deposited 2022-12-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
22–179（158 aa）
|
Not recorded | RLO (2R,3S,4R,5S,6R)-2-(hydroxymethyl)-6-[(E)-3-quinolin-6-ylprop-2-enyl]oxane-3,4,5-triol × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.5；293.15 K；3.0M NaCl and 0.1M BIS-TRIS at pH=5.5
|
Resolution 3.00 Å R-free 0.303 |
| 8BVD FimH lectin domain in complex with mannose C-linked to quinoline Deposited 2022-12-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
22–179（158 aa）
|
Not recorded | RLO (2R,3S,4R,5S,6R)-2-(hydroxymethyl)-6-[(E)-3-quinolin-6-ylprop-2-enyl]oxane-3,4,5-triol × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.5；293.15 K；3.0M NaCl and 0.1M BIS-TRIS at pH=5.5
|
Resolution 3.00 Å R-free 0.303 |
| 8BVD FimH lectin domain in complex with mannose C-linked to quinoline Deposited 2022-12-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
22–179（158 aa）
|
Not recorded | RLO (2R,3S,4R,5S,6R)-2-(hydroxymethyl)-6-[(E)-3-quinolin-6-ylprop-2-enyl]oxane-3,4,5-triol × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.5；293.15 K；3.0M NaCl and 0.1M BIS-TRIS at pH=5.5
|
Resolution 3.00 Å R-free 0.303 |
| 8BXY FimH in complex with alpha1,6 core-fucosylated oligomannose-3, crystallized in the trigonal space group Deposited 2022-12-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
22–179（158 aa）
|
Not recorded | NI NICKEL (II) ION × 1 SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 9；291 K；1.1 M Lithium sulphate; 0.1 M Tris-HCl pH 9.0; 10 mM nickel chloride
|
Resolution 1.45 Å R-free 0.205 |
| 8BXY FimH in complex with alpha1,6 core-fucosylated oligomannose-3, crystallized in the trigonal space group Deposited 2022-12-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Other combination Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
22–179（158 aa）
|
Not recorded | NI NICKEL (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 9；291 K；1.1 M Lithium sulphate; 0.1 M Tris-HCl pH 9.0; 10 mM nickel chloride
|
Resolution 1.45 Å R-free 0.205 |
| 8BY3 FimH lectin domain in complex with oligomannose-6 Deposited 2022-12-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
22–179（158 aa）
Chain C
22–179（158 aa）
|
Not recorded | NI NICKEL (II) ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；291 K；1 M Lithium sulphate
100 mM Tris-HCl, pH 8.5
10 mM Nickel chloride
3% glycerol
|
Resolution 3.19 Å R-free 0.238 |
| 8BY3 FimH lectin domain in complex with oligomannose-6 Deposited 2022-12-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Other combination Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain B
22–179（158 aa）
Chain D
22–179（158 aa）
|
Not recorded | NI NICKEL (II) ION × 2 SO4 SULFATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；291 K；1 M Lithium sulphate
100 mM Tris-HCl, pH 8.5
10 mM Nickel chloride
3% glycerol
|
Resolution 3.19 Å R-free 0.238 |
| 8V3J Structure-Based Engineering of a Highly Immunogenic, Conformationally Stabilized FimH Antigen for a Urinary Tract Infection Vaccine Deposited 2023-11-28 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
22–300（279 aa）
|
Mutation：N7S,G15A,G16A,V27A,N70S,N228Q | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 4.5；293 K；0.1 M Sodium Acetate (pH 4.5), 25% (w/v) PEG 3350
|
Resolution 1.90 Å R-free 0.238 |
| 8V3J Structure-Based Engineering of a Highly Immunogenic, Conformationally Stabilized FimH Antigen for a Urinary Tract Infection Vaccine Deposited 2023-11-28 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
22–300（279 aa）
|
Mutation：N7S,G15A,G16A,V27A,N70S,N228Q | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 4.5；293 K；0.1 M Sodium Acetate (pH 4.5), 25% (w/v) PEG 3350
|
Resolution 1.90 Å R-free 0.238 |
| 8V3J Structure-Based Engineering of a Highly Immunogenic, Conformationally Stabilized FimH Antigen for a Urinary Tract Infection Vaccine Deposited 2023-11-28 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
22–300（279 aa）
|
Mutation：N7S,G15A,G16A,V27A,N70S,N228Q | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 4.5；293 K；0.1 M Sodium Acetate (pH 4.5), 25% (w/v) PEG 3350
|
Resolution 1.90 Å R-free 0.238 |
| 8V3J Structure-Based Engineering of a Highly Immunogenic, Conformationally Stabilized FimH Antigen for a Urinary Tract Infection Vaccine Deposited 2023-11-28 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
22–300（279 aa）
|
Mutation：N7S,G15A,G16A,V27A,N70S,N228Q | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 4.5；293 K；0.1 M Sodium Acetate (pH 4.5), 25% (w/v) PEG 3350
|
Resolution 1.90 Å R-free 0.238 |
| 8V93 Cryo-EM structure of E. coli FimH lectin domain bound to Fabs 329-2 and 454-3 Deposited 2023-12-07 | Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 5 PDB declaration: pentameric |
Chain D
22–300（279 aa）
|
Mutation：N7S, G15A, G16A, V27A, N70S, N228Q within FimH | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.12 Å |
| 9BOG Structural basis for adhesin secretion by the outer-membrane usher in type 1 pili Deposited 2024-05-03 | Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain H
22–300（279 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.99 Å |
| 9D6F Cryo-EM structure of E. coli FimH lectin domain bound to Fabs 440-2 and 454-3 Deposited 2024-08-15 | Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 5 PDB declaration: pentameric |
Chain D
22–300（279 aa）
|
Mutation：N7S, G15A, G16A, V27A, N70S, N228Q within FimH | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.24 Å |
| 9FY9 Cryo-EM structure of the type 1 chaperone-usher pilus FimD-tip complex (FimDHGFC) - Conformer 1 Deposited 2024-07-03 | Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric |
Chain H
22–300（279 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 3.30 Å |
| 9ME4 Antibody fragments from mAb475 and mAb824 bound to the adhesin protein FimH Deposited 2024-12-06 | Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain A
1–300（300 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 9ME5 Antibody fragments from mAb824 and mAb926 bound to the adhesin protein FimH Deposited 2024-12-06 | Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric |
Chain A
1–300（300 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 9ME6 Antibody fragments from mAb21, mAb475, and mAb824 bound to the adhesin protein FimH Deposited 2024-12-06 | Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric |
Chain A
1–300（300 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 9ME7 Antibody fragments from mAb21 and mAb824 bound to the adhesin protein FimH containing alpha-methyl mannose Deposited 2024-12-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric |
Chain A
1–300（300 aa）
|
Not recorded | MMA methyl alpha-D-mannopyranoside × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 9PTM Antibody fragment from mAb824 bound to the adhesin protein FimH. Deposited 2025-07-29 | Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–300（300 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
57 other PDB entries and 137 assemblies. Open the comparison page and filter oligomeric states
View Construct and Data Evidence
| UniProt name | FIMH_ECOLI |
| Isoform | — |
| PDB entities | 2 |
| Chains and sequence ranges | Author chain B； PDBConstruct 1–160； UniProt 22–181 Author chain D； PDBConstruct 1–160； UniProt 22–181 |
