Current Protein Identity：P02550
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1FFX TUBULIN:STATHMIN-LIKE DOMAIN COMPLEX Deposited 2000-07-26 | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric（5） Consistent with protein count |
Chain A
1–451（451 aa）
Chain C
1–451（451 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；PEG, pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 293.K
|
Resolution 3.95 Å R-free 0.367 |
| 1IA0 KIF1A HEAD-MICROTUBULE COMPLEX STRUCTURE IN ATP-FORM Deposited 2001-03-22 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
1–451（451 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TXL TAXOTERE × 1 MG MAGNESIUM ION × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
buf1；pH 7.4；IMIDAZOLE 50 MM, MG-ACETATE 5 MM, EGTA 1 MM, K-ACETATE 50 MM, DTT 10 MM, PACLITAXEL 10 MICRO M
cryo-EM vitrification conditions
PLUNGED INTO ETHANE
X-ray crystallization conditions
polymerization of the microtubule；pH 6.8；296 K；pH 6.8, polymerization of the microtubule, temperature 296K
|
Resolution 15.00 Å |
| 1SA0 TUBULIN-COLCHICINE: STATHMIN-LIKE DOMAIN COMPLEX Deposited 2004-02-06 | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric（5） Consistent with protein count |
Chain A
1–451（451 aa）
Chain C
1–451（451 aa）
|
Not recorded | MG MAGNESIUM ION × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 CN2 2-MERCAPTO-N-[1,2,3,10-TETRAMETHOXY-9-OXO-5,6,7,9-TETRAHYDRO-BENZO[A]HEPTALEN-7-YL]ACETAMIDE × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；291 K；PEG, PIPES BUFFER, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K
|
Resolution 3.58 Å R-free 0.249 |
| 1SA1 Tubulin-podophyllotoxin: stathmin-like domain complex Deposited 2004-02-06 | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric（5） Consistent with protein count |
Chain A
1–451（451 aa）
Chain C
1–451（451 aa）
|
Not recorded | MG MAGNESIUM ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 POD 9-HYDROXY-5-(3,4,5-TRIMETHOXYPHENYL)-5,8,8A,9-TETRAHYDROFURO[3',4':6,7]NAPHTHO[2,3-D][1,3]DIOXOL-6(5AH)-ONE × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；291 K；PEG, PIPES BUFFER, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K
|
Resolution 4.20 Å R-free 0.259 |
| 1TUB TUBULIN ALPHA-BETA DIMER, ELECTRON DIFFRACTION Deposited 1997-09-23 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–440（440 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TXL TAXOTERE × 1 | ELECTRON CRYSTALLOGRAPHY |
X-ray crystallization conditions
TWO-DIMENSIONAL SHEETS ARE FORMED UNDER NORMAL POLYMERIZING CONDITIONS WITH THE ADDITION OF 1 MM ZNCL2
|
Resolution 3.70 Å |
| 1TVK The binding mode of epothilone A on a,b-tubulin by electron crystallography Deposited 2004-06-29 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–440（440 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 EP EPOTHILONE A × 1 | ELECTRON CRYSTALLOGRAPHY | mmCIF provides none of the parsed conditions | Resolution 2.89 Å R-free 0.321 |
| 2HXF KIF1A head-microtubule complex structure in amppnp-form Deposited 2006-08-03 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
1–451（451 aa）
|
Not recorded | MG MAGNESIUM ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
IMIDAZOLE；pH 7.4；IMIDAZOLE
cryo-EM vitrification conditions
Ethan slash
|
Resolution 10.00 Å |
| 2HXH KIF1A head-microtubule complex structure in adp-form Deposited 2006-08-03 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
1–451（451 aa）
|
Not recorded | MG MAGNESIUM ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
imidazole；pH 7.4；imidazole
cryo-EM vitrification conditions
Ethan slash
|
Resolution 11.00 Å |
| 2WBE Kinesin-5-Tubulin Complex with AMPPNP Deposited 2009-02-26 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1–451（451 aa）
|
Not recorded | MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
80MM PIPES, 150MM NACL, 7MM MGCL2, 1MM EGTA, 1MM BETA-MERCAPTOETHANOL；pH 6.8；80MM PIPES, 150MM NACL, 7MM MGCL2, 1MM EGTA, 1MM BETA-MERCAPTOETHANOL
cryo-EM vitrification conditions
ETHANE
|
Resolution 9.40 Å |
| 3EDL Kinesin13-Microtubule Ring complex Deposited 2008-09-03 | Assembly 1 Protein heterocomplex Heteromer；Protein × 165 PDB declaration: 165-meric（165） Consistent with protein count |
Chain A
1–451（451 aa）
Chain F
1–451（451 aa）
|
Not recorded | ZN ZINC ION × 33 MG MAGNESIUM ION × 99 GTP GUANOSINE-5'-TRIPHOSPHATE × 66 GDP GUANOSINE-5'-DIPHOSPHATE × 66 TA1 TAXOL × 33 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 33 CN2 2-MERCAPTO-N-[1,2,3,10-TETRAMETHOXY-9-OXO-5,6,7,9-TETRAHYDRO-BENZO[A]HEPTALEN-7-YL]ACETAMIDE × 33 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8；80 mM Pipes, pH 6.8, 2 mM MgCl2, 1 mM EGTA, 2 mM AMP-PNP, 0.02 mM taxol
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 28.00 Å |
| 3EDL Kinesin13-Microtubule Ring complex Deposited 2008-09-03 | Assembly 2 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric（5） Consistent with protein count |
Chain A
1–451（451 aa）
Chain F
1–451（451 aa）
|
Not recorded | ZN ZINC ION × 1 MG MAGNESIUM ION × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 TA1 TAXOL × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 CN2 2-MERCAPTO-N-[1,2,3,10-TETRAMETHOXY-9-OXO-5,6,7,9-TETRAHYDRO-BENZO[A]HEPTALEN-7-YL]ACETAMIDE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8；80 mM Pipes, pH 6.8, 2 mM MgCl2, 1 mM EGTA, 2 mM AMP-PNP, 0.02 mM taxol
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 28.00 Å |
| 3EDL Kinesin13-Microtubule Ring complex Deposited 2008-09-03 | Assembly 3 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric（5） Consistent with protein count |
Chain A
1–451（451 aa）
Chain F
1–451（451 aa）
|
Not recorded | ZN ZINC ION × 1 MG MAGNESIUM ION × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 TA1 TAXOL × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 CN2 2-MERCAPTO-N-[1,2,3,10-TETRAMETHOXY-9-OXO-5,6,7,9-TETRAHYDRO-BENZO[A]HEPTALEN-7-YL]ACETAMIDE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8；80 mM Pipes, pH 6.8, 2 mM MgCl2, 1 mM EGTA, 2 mM AMP-PNP, 0.02 mM taxol
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 28.00 Å |
| 3J6E Energy minimized average structure of Microtubules stabilized by GmpCpp Deposited 2014-02-18 | Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric（18） Consistent with protein count |
Chain A
1–439（439 aa）
Chain C
1–439（439 aa）
Chain E
1–439（439 aa）
Chain G
1–439（439 aa）
Chain I
1–439（439 aa）
Chain K
1–439（439 aa）
Chain M
1–439（439 aa）
Chain O
1–439（439 aa）
Chain Q
1–439（439 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 9 MG MAGNESIUM ION × 18 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 9 | ELECTRON MICROSCOPY |
cryo-EM buffer
80 mM PIPES, 1 mM EGTA, 1 mM MgCl2, 1 mM DTT, 0.05% Nonidet P-40；pH 6.8；80 mM PIPES, 1 mM EGTA, 1 mM MgCl2, 1 mM DTT, 0.05% Nonidet P-40
cryo-EM vitrification conditions
The grid was blotted for 2 seconds before plunging.；90.4 K；Cryogen ETHANE；The grid was blotted for 2 seconds before plunging into liquid ethane (FEI VITROBOT MARK II).
|
Resolution 4.70 Å |
| 3J6F Minimized average structure of GDP-bound dynamic microtubules Deposited 2014-02-19 | Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric（18） Consistent with protein count |
Chain A
1–439（439 aa）
Chain C
1–439（439 aa）
Chain E
1–439（439 aa）
Chain G
1–439（439 aa）
Chain I
1–439（439 aa）
Chain K
1–439（439 aa）
Chain M
1–439（439 aa）
Chain O
1–439（439 aa）
Chain Q
1–439（439 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 9 MG MAGNESIUM ION × 9 GDP GUANOSINE-5'-DIPHOSPHATE × 9 | ELECTRON MICROSCOPY |
cryo-EM buffer
80 mM PIPES, 1 mM EGTA, 1 mM MgCl2, 1 mM DTT, 0.05% Nonidet P-40；pH 6.8；80 mM PIPES, 1 mM EGTA, 1 mM MgCl2, 1 mM DTT, 0.05% Nonidet P-40
cryo-EM vitrification conditions
The grid was blotted for 2 seconds before plunging.；90.4 K；Cryogen ETHANE；The grid was blotted for 2 seconds before plunging into liquid ethane (FEI VITROBOT MARK II).
|
Resolution 4.90 Å |
| 3J6G Minimized average structure of microtubules stabilized by taxol Deposited 2014-02-19 | Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric（18） Consistent with protein count |
Chain A
1–439（439 aa）
Chain C
1–439（439 aa）
Chain E
1–439（439 aa）
Chain G
1–439（439 aa）
Chain I
1–439（439 aa）
Chain K
1–439（439 aa）
Chain M
1–439（439 aa）
Chain O
1–439（439 aa）
Chain Q
1–439（439 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 9 MG MAGNESIUM ION × 9 GDP GUANOSINE-5'-DIPHOSPHATE × 9 TA1 TAXOL × 9 | ELECTRON MICROSCOPY |
cryo-EM buffer
80 mM PIPES, 1 mM EGTA, 1 mM MgCl2, 1 mM DTT, 0.05% Nonidet P-40；pH 6.8；80 mM PIPES, 1 mM EGTA, 1 mM MgCl2, 1 mM DTT, 0.05% Nonidet P-40
cryo-EM vitrification conditions
The grid was blotted for 2 seconds before plunging.；90.4 K；Cryogen ETHANE；The grid was blotted for 2 seconds before plunging into liquid ethane (FEI VITROBOT MARK II).
|
Resolution 5.50 Å |
| 3J6H Nucleotide-free Kinesin motor domain complexed with GMPCPP-microtubule Deposited 2014-02-21 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
2–437（436 aa）
Fragment：UNP residues 2-437
|
Not recorded | MG MAGNESIUM ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 SO4 SULFATE ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 8.10 Å |
| 3J6P Pseudo-atomic model of dynein microtubule binding domain-tubulin complex based on a cryoEM map Deposited 2014-03-20 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
1–451（451 aa）
|
Not recorded | MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Blot for 3.5 seconds before plunging；Cryogen ETHANE
|
Resolution 8.20 Å |
| 3J7I Structure of alpha- and beta- tubulin in GMPCPP-microtubules Deposited 2014-07-01 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–451（451 aa）
|
Not recorded | MG MAGNESIUM ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 8.90 Å |
| 5MM4 Ustilago maydis kinesin-5 motor domain in the AMPPNP state bound to microtubules Deposited 2016-12-08 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
1–439（439 aa）
|
Not recorded | MG MAGNESIUM ION × 2 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 TA1 TAXOL × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.50 Å |
| 5MM7 Ustilago maydis kinesin-5 motor domain with N-terminal extension in the AMPPNP state bound to microtubules Deposited 2016-12-08 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
1–439（439 aa）
|
Not recorded | MG MAGNESIUM ION × 2 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 TA1 TAXOL × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.10 Å |
| 5XXT GDP-microtubule complexed with nucleotide-free KIF5C Deposited 2017-07-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric（18） Consistent with protein count |
Chain A
2–439（438 aa）
Fragment：UNP residues 2-439
Chain C
2–439（438 aa）
Fragment：UNP residues 2-439
Chain E
2–439（438 aa）
Fragment：UNP residues 2-439
Chain G
2–439（438 aa）
Fragment：UNP residues 2-439
Chain I
2–439（438 aa）
Fragment：UNP residues 2-439
Chain K
2–439（438 aa）
Fragment：UNP residues 2-439
Chain M
2–439（438 aa）
Fragment：UNP residues 2-439
Chain O
2–439（438 aa）
Fragment：UNP residues 2-439
Chain Q
2–439（438 aa）
Fragment：UNP residues 2-439
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 9 MG MAGNESIUM ION × 9 GDP GUANOSINE-5'-DIPHOSPHATE × 9 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.35 Å |
| 5XXV GDP-microtubule complexed with KIF5C in AMPPNP state Deposited 2017-07-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric（18） Consistent with protein count |
Chain A
2–439（438 aa）
Fragment：UNP residues 2-439
Chain C
2–439（438 aa）
Fragment：UNP residues 2-439
Chain E
2–439（438 aa）
Fragment：UNP residues 2-439
Chain G
2–439（438 aa）
Fragment：UNP residues 2-439
Chain I
2–439（438 aa）
Fragment：UNP residues 2-439
Chain K
2–439（438 aa）
Fragment：UNP residues 2-439
Chain M
2–439（438 aa）
Fragment：UNP residues 2-439
Chain O
2–439（438 aa）
Fragment：UNP residues 2-439
Chain Q
2–439（438 aa）
Fragment：UNP residues 2-439
|
Not recorded | MG MAGNESIUM ION × 9 GTP GUANOSINE-5'-TRIPHOSPHATE × 9 GDP GUANOSINE-5'-DIPHOSPHATE × 9 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.46 Å |
| 5XXW GDP-microtubule complexed with KIF5C in ATP state Deposited 2017-07-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric（18） Consistent with protein count |
Chain A
2–439（438 aa）
Fragment：UNP residues 2-439
Chain C
2–439（438 aa）
Fragment：UNP residues 2-439
Chain E
2–439（438 aa）
Fragment：UNP residues 2-439
Chain G
2–439（438 aa）
Fragment：UNP residues 2-439
Chain I
2–439（438 aa）
Fragment：UNP residues 2-439
Chain K
2–439（438 aa）
Fragment：UNP residues 2-439
Chain M
2–439（438 aa）
Fragment：UNP residues 2-439
Chain O
2–439（438 aa）
Fragment：UNP residues 2-439
Chain Q
2–439（438 aa）
Fragment：UNP residues 2-439
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 9 MG MAGNESIUM ION × 9 GDP GUANOSINE-5'-DIPHOSPHATE × 9 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.00 Å |
| 5XXX GMPCPP-microtubule complexed with nucleotide-free KIF5C Deposited 2017-07-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric（18） Consistent with protein count |
Chain A
2–439（438 aa）
Fragment：UNP residues 2-439
Chain C
2–439（438 aa）
Fragment：UNP residues 2-439
Chain E
2–439（438 aa）
Fragment：UNP residues 2-439
Chain G
2–439（438 aa）
Fragment：UNP residues 2-439
Chain I
2–439（438 aa）
Fragment：UNP residues 2-439
Chain K
2–439（438 aa）
Fragment：UNP residues 2-439
Chain M
2–439（438 aa）
Fragment：UNP residues 2-439
Chain O
2–439（438 aa）
Fragment：UNP residues 2-439
Chain Q
2–439（438 aa）
Fragment：UNP residues 2-439
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 9 MG MAGNESIUM ION × 18 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 9 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.43 Å |
| 6MLQ Cryo-EM structure of microtubule-bound Kif7 in the ADP state Deposited 2018-09-27 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
1–451（451 aa）
|
Not recorded | MG MAGNESIUM ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted from behind the grid for 2 seconds
|
Resolution 4.20 Å |
| 6MLR Cryo-EM structure of microtubule-bound Kif7 in the AMPPNP state Deposited 2018-09-27 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
1–451（451 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted from behind the grid for 2 seconds
|
Resolution 4.20 Å |
| 6MZE Structural Basis of Tubulin Recruitment and Assembly by Microtubule Polymerases with Tumor Overexpressed Gene (TOG) Domain Arrays Deposited 2018-11-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 14 PDB declaration: tetradecameric（14） Consistent with protein count |
Chain A
1–451（451 aa）
Chain C
1–451（451 aa）
Chain H
1–451（451 aa）
Chain J
1–451（451 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；50 mM PIPES, 100 mM MgCl2 [pH 7.0], and 10-15% PEG-8000
|
Resolution 3.60 Å R-free 0.245 |
| 6MZE Structural Basis of Tubulin Recruitment and Assembly by Microtubule Polymerases with Tumor Overexpressed Gene (TOG) Domain Arrays Deposited 2018-11-05 | Assembly 2 Protein heterocomplex Heteromer；Protein × 14 PDB declaration: tetradecameric（14） Consistent with protein count |
Chain O
1–451（451 aa）
Chain Q
1–451（451 aa）
Chain V
1–451（451 aa）
Chain X
1–451（451 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；50 mM PIPES, 100 mM MgCl2 [pH 7.0], and 10-15% PEG-8000
|
Resolution 3.60 Å R-free 0.245 |
| 6MZF Structural Basis of Tubulin Recruitment and Assembly by Microtubule Polymerases with Tumor Overexpressed Gene (TOG) Domain Arrays Deposited 2018-11-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 14 PDB declaration: tetradecameric（14） Consistent with protein count |
Chain A
1–451（451 aa）
Chain C
1–451（451 aa）
Chain H
1–451（451 aa）
Chain J
1–451（451 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；50 mM PIPES, 100 mM MgCl2 [pH 7.0], and 10-15% PEG-8000
|
Resolution 4.40 Å R-free 0.252 |
| 6MZF Structural Basis of Tubulin Recruitment and Assembly by Microtubule Polymerases with Tumor Overexpressed Gene (TOG) Domain Arrays Deposited 2018-11-05 | Assembly 2 Protein heterocomplex Heteromer；Protein × 14 PDB declaration: tetradecameric（14） Consistent with protein count |
Chain O
1–451（451 aa）
Chain Q
1–451（451 aa）
Chain V
1–451（451 aa）
Chain X
1–451（451 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；50 mM PIPES, 100 mM MgCl2 [pH 7.0], and 10-15% PEG-8000
|
Resolution 4.40 Å R-free 0.252 |
| 6MZG Structural Basis of Tubulin Recruitment and Assembly by Microtubule Polymerases with Tumor Overexpressed Gene (TOG) Domain Arrays Deposited 2018-11-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain A
1–451（451 aa）
Chain C
1–451（451 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 4 GDP GUANOSINE-5'-DIPHOSPHATE × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；50 mM PIPES, 100 mM MgCl2 [pH 7.0], and 10-15% PEG-8000
|
Resolution 3.21 Å R-free 0.234 |
| 6MZG Structural Basis of Tubulin Recruitment and Assembly by Microtubule Polymerases with Tumor Overexpressed Gene (TOG) Domain Arrays Deposited 2018-11-05 | Assembly 2 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain G
1–451（451 aa）
Chain I
1–451（451 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 4 GDP GUANOSINE-5'-DIPHOSPHATE × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；50 mM PIPES, 100 mM MgCl2 [pH 7.0], and 10-15% PEG-8000
|
Resolution 3.21 Å R-free 0.234 |
| 6VPO Cryo-EM structure of microtubule-bound KLP61F motor domain in the AMPPNP state Deposited 2020-02-04 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
1–451（451 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.40 Å |
| 6VPP Cryo-EM structure of microtubule-bound KLP61F motor with tail domain in the nucleotide-free state Deposited 2020-02-04 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
1–451（451 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.40 Å |
| 7RS5 Cryo-EM structure of Kip3 (AMPPNP) bound to Taxol-Stabilized Microtubules Deposited 2021-08-10 | Assembly 1 Protein heterocomplex Heteromer；Protein × 27 PDB declaration: 27-meric（27） Consistent with protein count |
Chain A
1–451（451 aa）
Chain C
1–451（451 aa）
Chain E
1–451（451 aa）
Chain G
1–451（451 aa）
Chain I
1–451（451 aa）
Chain L
1–451（451 aa）
Chain N
1–451（451 aa）
Chain P
1–451（451 aa）
Chain R
1–451（451 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 9 MG MAGNESIUM ION × 18 GDP GUANOSINE-5'-DIPHOSPHATE × 9 TA1 TAXOL × 9 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 9 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8；cryoEM buffer (50 mM Tris-HCl, pH 8.0, 1 mM MgCl2, 1 mM EGTA, 1 mM DTT supplemented with 2mM AMPPNP)
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.90 Å |
| 7RX0 Complex of AMPPNP-Kif7 and Gli2 Zinc-Finger domain bound to microtubules Deposited 2021-08-20 | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–451（451 aa）
|
Not recorded | ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted from behind the grid for 2 seconds
|
Resolution 3.89 Å |
| 7U0F HIV-1 Rev in complex with tubulin Deposited 2022-02-18 | Assembly 1 Protein heterocomplex Heteromer；Protein × 150 PDB declaration: 150-meric（150） Consistent with protein count |
Chain A
1–451（451 aa）
Chain C
1–451（451 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.53 Å |
| 7U0F HIV-1 Rev in complex with tubulin Deposited 2022-02-18 | Assembly 2 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric（10） Consistent with protein count |
Chain A
1–451（451 aa）
Chain C
1–451（451 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.53 Å |
| 7U0F HIV-1 Rev in complex with tubulin Deposited 2022-02-18 | Assembly 3 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric（10） Consistent with protein count |
Chain A
1–451（451 aa）
Chain C
1–451（451 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.53 Å |
| 7X4N Crystal Structure of C. elegans kinesin-4 KLP-12 complexed with tubulin and DARPin Deposited 2022-03-03 | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–451（451 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；ammonium acetate, HEPES, polyethylene glycol (PEG) 3350
|
Resolution 2.88 Å R-free 0.296 |
| 8QAU Outer kinetochore Ndc80-Dam1 alpha/beta-tubulin complex Deposited 2023-08-23 | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric（5） Consistent with protein count |
Chain C
1–451（451 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.54 Å |
| 9EDR Tubulin Cofactors D,E,G,C and Tubulin complex -- TBCC N Terminus Bound to Tubulin Deposited 2024-11-17 | Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric（7） Consistent with protein count |
Chain A
1–451（451 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 9EDS Tubulin cofactors D,E,G,C bound to tubulin dimer -- TBCC N terminus unbound Deposited 2024-11-18 | Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric（7） Consistent with protein count |
Chain A
1–451（451 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 9EDT Tubulin cofactors D,E,G bound to tubulin dimer Deposited 2024-11-18 | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain A
1–451（451 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 9EEB Tubulin cofactors D,E,G bound to tubulin dimer Deposited 2024-11-18 | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain A
1–451（451 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 9RPD D. melanogaster Augmin TII N-clamp (GST-fusion) bound to a microtubule, well-defined subset of particles Deposited 2025-06-24 | Assembly 1 Insufficient information Heteromer；Protein × 9 PDB declaration: nonameric（9） Consistent with protein count |
Chain A
1–451（451 aa）
Chain C
1–451（451 aa）
Chain E
1–451（451 aa）
Chain G
1–451（451 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.90 Å |
| 9T1D Cryo-EM reconstruction of undecorated GDP microtubule Deposited 2025-10-21 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–439（439 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 2.90 Å |