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6mlr

Cryo-EM structure of microtubule-bound Kif7 in the AMPPNP state

Method: ELECTRON MICROSCOPY Dmax: 110.1 Å Quality: GOOD
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Tubulin alpha-1A chain

OrganismNot specified

UniProt P02550

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 3 PDB declaration: trimeric（3） Consistent with protein copy count Chain A； UniProt 1–451 Not recorded Tubulin beta chain × 1 （P02554） Kinesin-like protein KIF7 × 1 （Q2M1P5） GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 6.8 cryo-EM vitrification conditions：Cryogen ETHANE；Blotted from behind the grid for 2 seconds Resolution 4.20 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 6MLR Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
1FFX TUBULIN:STATHMIN-LIKE DOMAIN COMPLEX Deposited 2000-07-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；PEG, pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 293.K
Resolution 3.95 Å R-free 0.367
1IA0 KIF1A HEAD-MICROTUBULE COMPLEX STRUCTURE IN ATP-FORM Deposited 2001-03-22 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TXL TAXOTERE × 1 MG MAGNESIUM ION × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer buf1；pH 7.4；IMIDAZOLE 50 MM, MG-ACETATE 5 MM, EGTA 1 MM, K-ACETATE 50 MM, DTT 10 MM, PACLITAXEL 10 MICRO M
cryo-EM vitrification conditions PLUNGED INTO ETHANE
X-ray crystallization conditions polymerization of the microtubule；pH 6.8；296 K；pH 6.8, polymerization of the microtubule, temperature 296K
Resolution 15.00 Å
1SA0 TUBULIN-COLCHICINE: STATHMIN-LIKE DOMAIN COMPLEX Deposited 2004-02-06 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Not recorded MG MAGNESIUM ION × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 CN2 2-MERCAPTO-N-[1,2,3,10-TETRAMETHOXY-9-OXO-5,6,7,9-TETRAHYDRO-BENZO[A]HEPTALEN-7-YL]ACETAMIDE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；291 K；PEG, PIPES BUFFER, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 3.58 Å R-free 0.249
1SA1 Tubulin-podophyllotoxin: stathmin-like domain complex Deposited 2004-02-06 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Not recorded MG MAGNESIUM ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 POD 9-HYDROXY-5-(3,4,5-TRIMETHOXYPHENYL)-5,8,8A,9-TETRAHYDROFURO[3',4':6,7]NAPHTHO[2,3-D][1,3]DIOXOL-6(5AH)-ONE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；291 K；PEG, PIPES BUFFER, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 4.20 Å R-free 0.259
1TUB TUBULIN ALPHA-BETA DIMER, ELECTRON DIFFRACTION Deposited 1997-09-23 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1–440（440 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TXL TAXOTERE × 1 ELECTRON CRYSTALLOGRAPHY
X-ray crystallization conditions TWO-DIMENSIONAL SHEETS ARE FORMED UNDER NORMAL POLYMERIZING CONDITIONS WITH THE ADDITION OF 1 MM ZNCL2
Resolution 3.70 Å
1TVK The binding mode of epothilone A on a,b-tubulin by electron crystallography Deposited 2004-06-29 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1–440（440 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 EP EPOTHILONE A × 1 ELECTRON CRYSTALLOGRAPHY mmCIF provides none of the parsed conditions Resolution 2.89 Å R-free 0.321
2HXF KIF1A head-microtubule complex structure in amppnp-form Deposited 2006-08-03 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–451（451 aa）
Not recorded MG MAGNESIUM ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer IMIDAZOLE；pH 7.4；IMIDAZOLE
cryo-EM vitrification conditions Ethan slash
Resolution 10.00 Å
2HXH KIF1A head-microtubule complex structure in adp-form Deposited 2006-08-03 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–451（451 aa）
Not recorded MG MAGNESIUM ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer imidazole；pH 7.4；imidazole
cryo-EM vitrification conditions Ethan slash
Resolution 11.00 Å
2WBE Kinesin-5-Tubulin Complex with AMPPNP Deposited 2009-02-26 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–451（451 aa）
Not recorded MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer 80MM PIPES, 150MM NACL, 7MM MGCL2, 1MM EGTA, 1MM BETA-MERCAPTOETHANOL；pH 6.8；80MM PIPES, 150MM NACL, 7MM MGCL2, 1MM EGTA, 1MM BETA-MERCAPTOETHANOL
cryo-EM vitrification conditions ETHANE
Resolution 9.40 Å
3EDL Kinesin13-Microtubule Ring complex Deposited 2008-09-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 165 PDB declaration: 165-meric
Chain A 1–451（451 aa）
Chain F 1–451（451 aa）
Not recorded ZN ZINC ION × 33 MG MAGNESIUM ION × 99 GTP GUANOSINE-5'-TRIPHOSPHATE × 66 GDP GUANOSINE-5'-DIPHOSPHATE × 66 TA1 TAXOL × 33 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 33 CN2 2-MERCAPTO-N-[1,2,3,10-TETRAMETHOXY-9-OXO-5,6,7,9-TETRAHYDRO-BENZO[A]HEPTALEN-7-YL]ACETAMIDE × 33 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8；80 mM Pipes, pH 6.8, 2 mM MgCl2, 1 mM EGTA, 2 mM AMP-PNP, 0.02 mM taxol
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 28.00 Å
3EDL Kinesin13-Microtubule Ring complex Deposited 2008-09-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain A 1–451（451 aa）
Chain F 1–451（451 aa）
Not recorded ZN ZINC ION × 1 MG MAGNESIUM ION × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 TA1 TAXOL × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 CN2 2-MERCAPTO-N-[1,2,3,10-TETRAMETHOXY-9-OXO-5,6,7,9-TETRAHYDRO-BENZO[A]HEPTALEN-7-YL]ACETAMIDE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8；80 mM Pipes, pH 6.8, 2 mM MgCl2, 1 mM EGTA, 2 mM AMP-PNP, 0.02 mM taxol
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 28.00 Å
3EDL Kinesin13-Microtubule Ring complex Deposited 2008-09-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain A 1–451（451 aa）
Chain F 1–451（451 aa）
Not recorded ZN ZINC ION × 1 MG MAGNESIUM ION × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 TA1 TAXOL × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 CN2 2-MERCAPTO-N-[1,2,3,10-TETRAMETHOXY-9-OXO-5,6,7,9-TETRAHYDRO-BENZO[A]HEPTALEN-7-YL]ACETAMIDE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8；80 mM Pipes, pH 6.8, 2 mM MgCl2, 1 mM EGTA, 2 mM AMP-PNP, 0.02 mM taxol
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 28.00 Å
3J6E Energy minimized average structure of Microtubules stabilized by GmpCpp Deposited 2014-02-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain A 1–439（439 aa）
Chain C 1–439（439 aa）
Chain E 1–439（439 aa）
Chain G 1–439（439 aa）
Chain I 1–439（439 aa）
Chain K 1–439（439 aa）
Chain M 1–439（439 aa）
Chain O 1–439（439 aa）
Chain Q 1–439（439 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 9 MG MAGNESIUM ION × 18 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 9 ELECTRON MICROSCOPY
cryo-EM buffer 80 mM PIPES, 1 mM EGTA, 1 mM MgCl2, 1 mM DTT, 0.05% Nonidet P-40；pH 6.8；80 mM PIPES, 1 mM EGTA, 1 mM MgCl2, 1 mM DTT, 0.05% Nonidet P-40
cryo-EM vitrification conditions The grid was blotted for 2 seconds before plunging.；90.4 K；Cryogen ETHANE；The grid was blotted for 2 seconds before plunging into liquid ethane (FEI VITROBOT MARK II).
Resolution 4.70 Å
3J6F Minimized average structure of GDP-bound dynamic microtubules Deposited 2014-02-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain A 1–439（439 aa）
Chain C 1–439（439 aa）
Chain E 1–439（439 aa）
Chain G 1–439（439 aa）
Chain I 1–439（439 aa）
Chain K 1–439（439 aa）
Chain M 1–439（439 aa）
Chain O 1–439（439 aa）
Chain Q 1–439（439 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 9 MG MAGNESIUM ION × 9 GDP GUANOSINE-5'-DIPHOSPHATE × 9 ELECTRON MICROSCOPY
cryo-EM buffer 80 mM PIPES, 1 mM EGTA, 1 mM MgCl2, 1 mM DTT, 0.05% Nonidet P-40；pH 6.8；80 mM PIPES, 1 mM EGTA, 1 mM MgCl2, 1 mM DTT, 0.05% Nonidet P-40
cryo-EM vitrification conditions The grid was blotted for 2 seconds before plunging.；90.4 K；Cryogen ETHANE；The grid was blotted for 2 seconds before plunging into liquid ethane (FEI VITROBOT MARK II).
Resolution 4.90 Å
3J6G Minimized average structure of microtubules stabilized by taxol Deposited 2014-02-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain A 1–439（439 aa）
Chain C 1–439（439 aa）
Chain E 1–439（439 aa）
Chain G 1–439（439 aa）
Chain I 1–439（439 aa）
Chain K 1–439（439 aa）
Chain M 1–439（439 aa）
Chain O 1–439（439 aa）
Chain Q 1–439（439 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 9 MG MAGNESIUM ION × 9 GDP GUANOSINE-5'-DIPHOSPHATE × 9 TA1 TAXOL × 9 ELECTRON MICROSCOPY
cryo-EM buffer 80 mM PIPES, 1 mM EGTA, 1 mM MgCl2, 1 mM DTT, 0.05% Nonidet P-40；pH 6.8；80 mM PIPES, 1 mM EGTA, 1 mM MgCl2, 1 mM DTT, 0.05% Nonidet P-40
cryo-EM vitrification conditions The grid was blotted for 2 seconds before plunging.；90.4 K；Cryogen ETHANE；The grid was blotted for 2 seconds before plunging into liquid ethane (FEI VITROBOT MARK II).
Resolution 5.50 Å
3J6H Nucleotide-free Kinesin motor domain complexed with GMPCPP-microtubule Deposited 2014-02-21 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 2–437（436 aa） Fragment：UNP residues 2-437
Not recorded MG MAGNESIUM ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 SO4 SULFATE ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 8.10 Å
3J6P Pseudo-atomic model of dynein microtubule binding domain-tubulin complex based on a cryoEM map Deposited 2014-03-20 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–451（451 aa）
Not recorded MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Blot for 3.5 seconds before plunging；Cryogen ETHANE
Resolution 8.20 Å
3J7I Structure of alpha- and beta- tubulin in GMPCPP-microtubules Deposited 2014-07-01 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1–451（451 aa）
Not recorded MG MAGNESIUM ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 8.90 Å
5MM4 Ustilago maydis kinesin-5 motor domain in the AMPPNP state bound to microtubules Deposited 2016-12-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–439（439 aa）
Not recorded MG MAGNESIUM ION × 2 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 TA1 TAXOL × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.50 Å
5MM7 Ustilago maydis kinesin-5 motor domain with N-terminal extension in the AMPPNP state bound to microtubules Deposited 2016-12-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–439（439 aa）
Not recorded MG MAGNESIUM ION × 2 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 TA1 TAXOL × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.10 Å
5XXT GDP-microtubule complexed with nucleotide-free KIF5C Deposited 2017-07-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain A 2–439（438 aa） Fragment：UNP residues 2-439
Chain C 2–439（438 aa） Fragment：UNP residues 2-439
Chain E 2–439（438 aa） Fragment：UNP residues 2-439
Chain G 2–439（438 aa） Fragment：UNP residues 2-439
Chain I 2–439（438 aa） Fragment：UNP residues 2-439
Chain K 2–439（438 aa） Fragment：UNP residues 2-439
Chain M 2–439（438 aa） Fragment：UNP residues 2-439
Chain O 2–439（438 aa） Fragment：UNP residues 2-439
Chain Q 2–439（438 aa） Fragment：UNP residues 2-439
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 9 MG MAGNESIUM ION × 9 GDP GUANOSINE-5'-DIPHOSPHATE × 9 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.35 Å
5XXV GDP-microtubule complexed with KIF5C in AMPPNP state Deposited 2017-07-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain A 2–439（438 aa） Fragment：UNP residues 2-439
Chain C 2–439（438 aa） Fragment：UNP residues 2-439
Chain E 2–439（438 aa） Fragment：UNP residues 2-439
Chain G 2–439（438 aa） Fragment：UNP residues 2-439
Chain I 2–439（438 aa） Fragment：UNP residues 2-439
Chain K 2–439（438 aa） Fragment：UNP residues 2-439
Chain M 2–439（438 aa） Fragment：UNP residues 2-439
Chain O 2–439（438 aa） Fragment：UNP residues 2-439
Chain Q 2–439（438 aa） Fragment：UNP residues 2-439
Not recorded MG MAGNESIUM ION × 9 GTP GUANOSINE-5'-TRIPHOSPHATE × 9 GDP GUANOSINE-5'-DIPHOSPHATE × 9 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.46 Å
5XXW GDP-microtubule complexed with KIF5C in ATP state Deposited 2017-07-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain A 2–439（438 aa） Fragment：UNP residues 2-439
Chain C 2–439（438 aa） Fragment：UNP residues 2-439
Chain E 2–439（438 aa） Fragment：UNP residues 2-439
Chain G 2–439（438 aa） Fragment：UNP residues 2-439
Chain I 2–439（438 aa） Fragment：UNP residues 2-439
Chain K 2–439（438 aa） Fragment：UNP residues 2-439
Chain M 2–439（438 aa） Fragment：UNP residues 2-439
Chain O 2–439（438 aa） Fragment：UNP residues 2-439
Chain Q 2–439（438 aa） Fragment：UNP residues 2-439
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 9 MG MAGNESIUM ION × 9 GDP GUANOSINE-5'-DIPHOSPHATE × 9 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.00 Å
5XXX GMPCPP-microtubule complexed with nucleotide-free KIF5C Deposited 2017-07-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain A 2–439（438 aa） Fragment：UNP residues 2-439
Chain C 2–439（438 aa） Fragment：UNP residues 2-439
Chain E 2–439（438 aa） Fragment：UNP residues 2-439
Chain G 2–439（438 aa） Fragment：UNP residues 2-439
Chain I 2–439（438 aa） Fragment：UNP residues 2-439
Chain K 2–439（438 aa） Fragment：UNP residues 2-439
Chain M 2–439（438 aa） Fragment：UNP residues 2-439
Chain O 2–439（438 aa） Fragment：UNP residues 2-439
Chain Q 2–439（438 aa） Fragment：UNP residues 2-439
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 9 MG MAGNESIUM ION × 18 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 9 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.43 Å
6MLQ Cryo-EM structure of microtubule-bound Kif7 in the ADP state Deposited 2018-09-27 Different ligand/ion Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–451（451 aa）
Not recorded MG MAGNESIUM ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE；Blotted from behind the grid for 2 seconds
Resolution 4.20 Å
6MZE Structural Basis of Tubulin Recruitment and Assembly by Microtubule Polymerases with Tumor Overexpressed Gene (TOG) Domain Arrays Deposited 2018-11-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 14 PDB declaration: tetradecameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Chain H 1–451（451 aa）
Chain J 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；50 mM PIPES, 100 mM MgCl2 [pH 7.0], and 10-15% PEG-8000
Resolution 3.60 Å R-free 0.245
6MZE Structural Basis of Tubulin Recruitment and Assembly by Microtubule Polymerases with Tumor Overexpressed Gene (TOG) Domain Arrays Deposited 2018-11-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 14 PDB declaration: tetradecameric
Chain O 1–451（451 aa）
Chain Q 1–451（451 aa）
Chain V 1–451（451 aa）
Chain X 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；50 mM PIPES, 100 mM MgCl2 [pH 7.0], and 10-15% PEG-8000
Resolution 3.60 Å R-free 0.245
6MZF Structural Basis of Tubulin Recruitment and Assembly by Microtubule Polymerases with Tumor Overexpressed Gene (TOG) Domain Arrays Deposited 2018-11-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 14 PDB declaration: tetradecameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Chain H 1–451（451 aa）
Chain J 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；50 mM PIPES, 100 mM MgCl2 [pH 7.0], and 10-15% PEG-8000
Resolution 4.40 Å R-free 0.252
6MZF Structural Basis of Tubulin Recruitment and Assembly by Microtubule Polymerases with Tumor Overexpressed Gene (TOG) Domain Arrays Deposited 2018-11-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 14 PDB declaration: tetradecameric
Chain O 1–451（451 aa）
Chain Q 1–451（451 aa）
Chain V 1–451（451 aa）
Chain X 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；50 mM PIPES, 100 mM MgCl2 [pH 7.0], and 10-15% PEG-8000
Resolution 4.40 Å R-free 0.252
6MZG Structural Basis of Tubulin Recruitment and Assembly by Microtubule Polymerases with Tumor Overexpressed Gene (TOG) Domain Arrays Deposited 2018-11-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 4 GDP GUANOSINE-5'-DIPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；50 mM PIPES, 100 mM MgCl2 [pH 7.0], and 10-15% PEG-8000
Resolution 3.21 Å R-free 0.234
6MZG Structural Basis of Tubulin Recruitment and Assembly by Microtubule Polymerases with Tumor Overexpressed Gene (TOG) Domain Arrays Deposited 2018-11-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain G 1–451（451 aa）
Chain I 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 4 GDP GUANOSINE-5'-DIPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；50 mM PIPES, 100 mM MgCl2 [pH 7.0], and 10-15% PEG-8000
Resolution 3.21 Å R-free 0.234
6VPO Cryo-EM structure of microtubule-bound KLP61F motor domain in the AMPPNP state Deposited 2020-02-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.40 Å
6VPP Cryo-EM structure of microtubule-bound KLP61F motor with tail domain in the nucleotide-free state Deposited 2020-02-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.40 Å
7RS5 Cryo-EM structure of Kip3 (AMPPNP) bound to Taxol-Stabilized Microtubules Deposited 2021-08-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 27 PDB declaration: 27-meric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Chain E 1–451（451 aa）
Chain G 1–451（451 aa）
Chain I 1–451（451 aa）
Chain L 1–451（451 aa）
Chain N 1–451（451 aa）
Chain P 1–451（451 aa）
Chain R 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 9 MG MAGNESIUM ION × 18 GDP GUANOSINE-5'-DIPHOSPHATE × 9 TA1 TAXOL × 9 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 9 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；cryoEM buffer (50 mM Tris-HCl, pH 8.0, 1 mM MgCl2, 1 mM EGTA, 1 mM DTT supplemented with 2mM AMPPNP)
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
7RX0 Complex of AMPPNP-Kif7 and Gli2 Zinc-Finger domain bound to microtubules Deposited 2021-08-20 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain A 1–451（451 aa）
Not recorded ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE；Blotted from behind the grid for 2 seconds
Resolution 3.89 Å
7U0F HIV-1 Rev in complex with tubulin Deposited 2022-02-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 150 PDB declaration: 150-meric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.53 Å
7U0F HIV-1 Rev in complex with tubulin Deposited 2022-02-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.53 Å
7U0F HIV-1 Rev in complex with tubulin Deposited 2022-02-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.53 Å
7X4N Crystal Structure of C. elegans kinesin-4 KLP-12 complexed with tubulin and DARPin Deposited 2022-03-03 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain A 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；ammonium acetate, HEPES, polyethylene glycol (PEG) 3350
Resolution 2.88 Å R-free 0.296
8QAU Outer kinetochore Ndc80-Dam1 alpha/beta-tubulin complex Deposited 2023-08-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain C 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.54 Å
9EDR Tubulin Cofactors D,E,G,C and Tubulin complex -- TBCC N Terminus Bound to Tubulin Deposited 2024-11-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain A 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
9EDS Tubulin cofactors D,E,G,C bound to tubulin dimer -- TBCC N terminus unbound Deposited 2024-11-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain A 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
9EDT Tubulin cofactors D,E,G bound to tubulin dimer Deposited 2024-11-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
9EEB Tubulin cofactors D,E,G bound to tubulin dimer Deposited 2024-11-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–451（451 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
9RPD D. melanogaster Augmin TII N-clamp (GST-fusion) bound to a microtubule, well-defined subset of particles Deposited 2025-06-24 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 9 PDB declaration: nonameric
Chain A 1–451（451 aa）
Chain C 1–451（451 aa）
Chain E 1–451（451 aa）
Chain G 1–451（451 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.90 Å
9T1D Cryo-EM reconstruction of undecorated GDP microtubule Deposited 2025-10-21 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1–439（439 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.90 Å

39 other PDB entries and 46 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name TBA1A_PIG
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 1–451； UniProt 1–451

Tubulin beta chain

OrganismNot specified

UniProt P02554

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 3 PDB declaration: trimeric（3） Consistent with protein copy count Chain B； UniProt 1–445 Not recorded Tubulin alpha-1A chain × 1 （P02550） Kinesin-like protein KIF7 × 1 （Q2M1P5） GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 6.8 cryo-EM vitrification conditions：Cryogen ETHANE；Blotted from behind the grid for 2 seconds Resolution 4.20 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 6MLR Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
1FFX TUBULIN:STATHMIN-LIKE DOMAIN COMPLEX Deposited 2000-07-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；PEG, pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 293.K
Resolution 3.95 Å R-free 0.367
1IA0 KIF1A HEAD-MICROTUBULE COMPLEX STRUCTURE IN ATP-FORM Deposited 2001-03-22 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TXL TAXOTERE × 1 MG MAGNESIUM ION × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer buf1；pH 7.4；IMIDAZOLE 50 MM, MG-ACETATE 5 MM, EGTA 1 MM, K-ACETATE 50 MM, DTT 10 MM, PACLITAXEL 10 MICRO M
cryo-EM vitrification conditions PLUNGED INTO ETHANE
X-ray crystallization conditions polymerization of the microtubule；pH 6.8；296 K；pH 6.8, polymerization of the microtubule, temperature 296K
Resolution 15.00 Å
1SA0 TUBULIN-COLCHICINE: STATHMIN-LIKE DOMAIN COMPLEX Deposited 2004-02-06 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Not recorded MG MAGNESIUM ION × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 CN2 2-MERCAPTO-N-[1,2,3,10-TETRAMETHOXY-9-OXO-5,6,7,9-TETRAHYDRO-BENZO[A]HEPTALEN-7-YL]ACETAMIDE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；291 K；PEG, PIPES BUFFER, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 3.58 Å R-free 0.249
1SA1 Tubulin-podophyllotoxin: stathmin-like domain complex Deposited 2004-02-06 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Chain E 49–189（141 aa）
Not recorded MG MAGNESIUM ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 POD 9-HYDROXY-5-(3,4,5-TRIMETHOXYPHENYL)-5,8,8A,9-TETRAHYDROFURO[3',4':6,7]NAPHTHO[2,3-D][1,3]DIOXOL-6(5AH)-ONE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；291 K；PEG, PIPES BUFFER, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 4.20 Å R-free 0.259
1TUB TUBULIN ALPHA-BETA DIMER, ELECTRON DIFFRACTION Deposited 1997-09-23 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 1–427（427 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TXL TAXOTERE × 1 ELECTRON CRYSTALLOGRAPHY
X-ray crystallization conditions TWO-DIMENSIONAL SHEETS ARE FORMED UNDER NORMAL POLYMERIZING CONDITIONS WITH THE ADDITION OF 1 MM ZNCL2
Resolution 3.70 Å
1TVK The binding mode of epothilone A on a,b-tubulin by electron crystallography Deposited 2004-06-29 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 1–427（427 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 EP EPOTHILONE A × 1 ELECTRON CRYSTALLOGRAPHY mmCIF provides none of the parsed conditions Resolution 2.89 Å R-free 0.321
2HXF KIF1A head-microtubule complex structure in amppnp-form Deposited 2006-08-03 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 1–445（445 aa）
Not recorded MG MAGNESIUM ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer IMIDAZOLE；pH 7.4；IMIDAZOLE
cryo-EM vitrification conditions Ethan slash
Resolution 10.00 Å
2HXH KIF1A head-microtubule complex structure in adp-form Deposited 2006-08-03 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 1–445（445 aa）
Not recorded MG MAGNESIUM ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer imidazole；pH 7.4；imidazole
cryo-EM vitrification conditions Ethan slash
Resolution 11.00 Å
2WBE Kinesin-5-Tubulin Complex with AMPPNP Deposited 2009-02-26 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 1–445（445 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ELECTRON MICROSCOPY
cryo-EM buffer 80MM PIPES, 150MM NACL, 7MM MGCL2, 1MM EGTA, 1MM BETA-MERCAPTOETHANOL；pH 6.8；80MM PIPES, 150MM NACL, 7MM MGCL2, 1MM EGTA, 1MM BETA-MERCAPTOETHANOL
cryo-EM vitrification conditions ETHANE
Resolution 9.40 Å
3EDL Kinesin13-Microtubule Ring complex Deposited 2008-09-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 165 PDB declaration: 165-meric
Chain B 1–445（445 aa）
Chain G 1–445（445 aa）
Not recorded ZN ZINC ION × 33 MG MAGNESIUM ION × 99 GTP GUANOSINE-5'-TRIPHOSPHATE × 66 GDP GUANOSINE-5'-DIPHOSPHATE × 66 TA1 TAXOL × 33 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 33 CN2 2-MERCAPTO-N-[1,2,3,10-TETRAMETHOXY-9-OXO-5,6,7,9-TETRAHYDRO-BENZO[A]HEPTALEN-7-YL]ACETAMIDE × 33 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8；80 mM Pipes, pH 6.8, 2 mM MgCl2, 1 mM EGTA, 2 mM AMP-PNP, 0.02 mM taxol
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 28.00 Å
3EDL Kinesin13-Microtubule Ring complex Deposited 2008-09-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain B 1–445（445 aa）
Chain G 1–445（445 aa）
Not recorded ZN ZINC ION × 1 MG MAGNESIUM ION × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 TA1 TAXOL × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 CN2 2-MERCAPTO-N-[1,2,3,10-TETRAMETHOXY-9-OXO-5,6,7,9-TETRAHYDRO-BENZO[A]HEPTALEN-7-YL]ACETAMIDE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8；80 mM Pipes, pH 6.8, 2 mM MgCl2, 1 mM EGTA, 2 mM AMP-PNP, 0.02 mM taxol
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 28.00 Å
3EDL Kinesin13-Microtubule Ring complex Deposited 2008-09-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain B 1–445（445 aa）
Chain G 1–445（445 aa）
Not recorded ZN ZINC ION × 1 MG MAGNESIUM ION × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 TA1 TAXOL × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 CN2 2-MERCAPTO-N-[1,2,3,10-TETRAMETHOXY-9-OXO-5,6,7,9-TETRAHYDRO-BENZO[A]HEPTALEN-7-YL]ACETAMIDE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8；80 mM Pipes, pH 6.8, 2 mM MgCl2, 1 mM EGTA, 2 mM AMP-PNP, 0.02 mM taxol
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 28.00 Å
3J6E Energy minimized average structure of Microtubules stabilized by GmpCpp Deposited 2014-02-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain B 1–427（427 aa）
Chain D 1–427（427 aa）
Chain F 1–427（427 aa）
Chain H 1–427（427 aa）
Chain J 1–427（427 aa）
Chain L 1–427（427 aa）
Chain N 1–427（427 aa）
Chain P 1–427（427 aa）
Chain R 1–427（427 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 9 MG MAGNESIUM ION × 18 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 9 ELECTRON MICROSCOPY
cryo-EM buffer 80 mM PIPES, 1 mM EGTA, 1 mM MgCl2, 1 mM DTT, 0.05% Nonidet P-40；pH 6.8；80 mM PIPES, 1 mM EGTA, 1 mM MgCl2, 1 mM DTT, 0.05% Nonidet P-40
cryo-EM vitrification conditions The grid was blotted for 2 seconds before plunging.；90.4 K；Cryogen ETHANE；The grid was blotted for 2 seconds before plunging into liquid ethane (FEI VITROBOT MARK II).
Resolution 4.70 Å
3J6F Minimized average structure of GDP-bound dynamic microtubules Deposited 2014-02-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain B 1–427（427 aa）
Chain D 1–427（427 aa）
Chain F 1–427（427 aa）
Chain H 1–427（427 aa）
Chain J 1–427（427 aa）
Chain L 1–427（427 aa）
Chain N 1–427（427 aa）
Chain P 1–427（427 aa）
Chain R 1–427（427 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 9 MG MAGNESIUM ION × 9 GDP GUANOSINE-5'-DIPHOSPHATE × 9 ELECTRON MICROSCOPY
cryo-EM buffer 80 mM PIPES, 1 mM EGTA, 1 mM MgCl2, 1 mM DTT, 0.05% Nonidet P-40；pH 6.8；80 mM PIPES, 1 mM EGTA, 1 mM MgCl2, 1 mM DTT, 0.05% Nonidet P-40
cryo-EM vitrification conditions The grid was blotted for 2 seconds before plunging.；90.4 K；Cryogen ETHANE；The grid was blotted for 2 seconds before plunging into liquid ethane (FEI VITROBOT MARK II).
Resolution 4.90 Å
3J6G Minimized average structure of microtubules stabilized by taxol Deposited 2014-02-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain B 1–427（427 aa）
Chain D 1–427（427 aa）
Chain F 1–427（427 aa）
Chain H 1–427（427 aa）
Chain J 1–427（427 aa）
Chain L 1–427（427 aa）
Chain N 1–427（427 aa）
Chain P 1–427（427 aa）
Chain R 1–427（427 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 9 MG MAGNESIUM ION × 9 GDP GUANOSINE-5'-DIPHOSPHATE × 9 TA1 TAXOL × 9 ELECTRON MICROSCOPY
cryo-EM buffer 80 mM PIPES, 1 mM EGTA, 1 mM MgCl2, 1 mM DTT, 0.05% Nonidet P-40；pH 6.8；80 mM PIPES, 1 mM EGTA, 1 mM MgCl2, 1 mM DTT, 0.05% Nonidet P-40
cryo-EM vitrification conditions The grid was blotted for 2 seconds before plunging.；90.4 K；Cryogen ETHANE；The grid was blotted for 2 seconds before plunging into liquid ethane (FEI VITROBOT MARK II).
Resolution 5.50 Å
3J6H Nucleotide-free Kinesin motor domain complexed with GMPCPP-microtubule Deposited 2014-02-21 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 2–427（426 aa） Fragment：UNP residues 2-427
Not recorded MG MAGNESIUM ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 SO4 SULFATE ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 8.10 Å
3J6P Pseudo-atomic model of dynein microtubule binding domain-tubulin complex based on a cryoEM map Deposited 2014-03-20 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 1–445（445 aa）
Not recorded MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Blot for 3.5 seconds before plunging；Cryogen ETHANE
Resolution 8.20 Å
3J7I Structure of alpha- and beta- tubulin in GMPCPP-microtubules Deposited 2014-07-01 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 1–445（445 aa）
Not recorded MG MAGNESIUM ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 8.90 Å
3JAK Cryo-EM structure of GTPgammaS-microtubule co-polymerized with EB3 (merged dataset with and without kinesin bound) Deposited 2015-06-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 14 PDB declaration: tetradecameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Chain F 1–445（445 aa）
Chain G 1–445（445 aa）
Chain H 1–445（445 aa）
Chain I 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 6 GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 6 ELECTRON MICROSCOPY
cryo-EM buffer BRB80；pH 6.8；80 mM PIPES, 1 mM EGTA, 1 mM MgCl2, 1 mM DTT, 0.05% Nonidet P-40
cryo-EM vitrification conditions Blot once for 4 seconds before plunging.；90.4 K；Cryogen ETHANE；Blot once for 4 seconds before plunging into liquid ethane (FEI VITROBOT MARK II).
Resolution 3.30 Å
3JAL Cryo-EM structure of GMPCPP-microtubule co-polymerized with EB3 Deposited 2015-06-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 14 PDB declaration: tetradecameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Chain F 1–445（445 aa）
Chain G 1–445（445 aa）
Chain H 1–445（445 aa）
Chain I 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 6 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 6 ELECTRON MICROSCOPY
cryo-EM buffer BRB80；pH 6.8；80 mM PIPES, 1 mM EGTA, 1 mM MgCl2, 1 mM DTT, 0.05% Nonidet P-40
cryo-EM vitrification conditions Blot once for 4 seconds before plunging；90.4 K；Cryogen ETHANE；Blot once for 4 seconds before plunging into liquid ethane (FEI VITROBOT MARK II).
Resolution 3.50 Å
3JAR Cryo-EM structure of GDP-microtubule co-polymerized with EB3 Deposited 2015-06-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 14 PDB declaration: tetradecameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Chain F 1–445（445 aa）
Chain G 1–445（445 aa）
Chain H 1–445（445 aa）
Chain I 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 6 ELECTRON MICROSCOPY
cryo-EM buffer BRB80；pH 6.8；80 mM PIPES, 1 mM EGTA, 1 mM MgCl2, 1 mM DTT, 0.05% Nonidet P-40
cryo-EM vitrification conditions Blot once for 4 seconds before plunging/；90.4 K；Cryogen ETHANE；Blot once for 4 seconds before plunging into liquid ethane (FEI VITROBOT MARK II).
Resolution 3.40 Å
3JAS Cryo-EM structure of dynamic GDP-microtubule (14 protofilaments) decorated with kinesin Deposited 2015-06-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Chain F 1–445（445 aa）
Chain G 1–445（445 aa）
Chain H 1–445（445 aa）
Chain I 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 6 ELECTRON MICROSCOPY
cryo-EM buffer BRB80；pH 6.8；80 mM PIPES, 1 mM EGTA, 1 mM MgCl2, 1 mM DTT, 0.05% Nonidet P-40
cryo-EM vitrification conditions Blot once for 4 seconds before plunging.；90.4 K；Cryogen ETHANE；Blot once for 4 seconds before plunging into liquid ethane (FEI VITROBOT MARK II).
Resolution 3.50 Å
3JAT Cryo-EM structure of GMPCPP-microtubule (14 protofilaments) decorated with kinesin Deposited 2015-06-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Chain F 1–445（445 aa）
Chain G 1–445（445 aa）
Chain H 1–445（445 aa）
Chain I 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 12 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 6 ELECTRON MICROSCOPY
cryo-EM buffer BRB80；pH 6.8；80 mM PIPES, 1 mM EGTA, 1 mM MgCl2, 1 mM DTT, 0.05% Nonidet P-40
cryo-EM vitrification conditions Blot once for 4 seconds before plunging.；90.4 K；Cryogen ETHANE；Blot once for 4 seconds before plunging into liquid ethane (FEI VITROBOT MARK II).
Resolution 3.50 Å
3JAW Atomic model of a microtubule seam based on a cryo-EM reconstruction of the EB3-bound microtubule (merged dataset containing tubulin bound to GTPgammaS, GMPCPP, and GDP) Deposited 2015-06-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer BRB80；pH 6.8；80 mM PIPES, 1 mM EGTA, 1 mM MgCl2, 1 mM DTT, 0.05% Nonidet P-40
cryo-EM vitrification conditions Blot once for 4 seconds before plunging.；90.4 K；Cryogen ETHANE；Blot once for 4 seconds before plunging into liquid ethane (FEI VITROBOT MARK II).
Resolution 3.90 Å
4ZHQ Crystal structure of Tubulin-Stathmin-TTL-MMAE Complex Deposited 2015-04-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 4 CA CALCIUM ION × 3 GOL GLYCEROL × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 4Q5 N-methyl-L-valyl-N-[(3R,4S,5S)-1-{(2S)-2-[(1R,2R)-3-{[(1S,2R)-1-hydroxy-1-phenylpropan-2-yl]amino}-1-methoxy-2-methyl-3-oxopropyl]pyrrolidin-1-yl}-3-methoxy-5-methyl-1-oxoheptan-4-yl]-N-methyl-L-valinamide × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 6.7；293 K；6% PEG, 5% glycerol, 0.1M MES, 30 mM CaCl2, 30 mM MgCl2
Resolution 2.55 Å R-free 0.245
4ZI7 CRYSTAL STRUCTURE OF TUBULIN-STATHMIN-TTL-HTI286 COMPLEX Deposited 2015-04-27 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 4 CA CALCIUM ION × 4 GOL GLYCEROL × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 4SL N,beta,beta-trimethyl-L-phenylalanyl-N-[(3S,4Z)-5-carboxy-2-methylhex-4-en-3-yl]-N,3-dimethyl-L-valinamide × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；293 K；PEG, glycerol, MES, CaCl2, MgCl2
Resolution 2.51 Å R-free 0.239
4ZOL Crystal Structure of Tubulin-Stathmin-TTL-Tubulysin M Complex Deposited 2015-05-06 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 4 CA CALCIUM ION × 2 GOL GLYCEROL × 7 GDP GUANOSINE-5'-DIPHOSPHATE × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 55Q (2R,4R)-4-{[(2-{(1R,3R)-1-(acetyloxy)-4-methyl-3-[methyl(N-{[(2S)-1-methylpiperidin-2-yl]carbonyl}-D-isoleucyl)amino]pentyl}-1,3-thiazol-4-yl)carbonyl]amino}-2-methyl-5-phenylpentanoic acid × 2 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 6.7；293 K；6% PEG, 5% glycerol, 0.1M MES, 30 mM CaCl2, 30 mM MgCl2
Resolution 2.50 Å R-free 0.232
5BMV CRYSTAL STRUCTURE OF TUBULIN-STATHMIN-TTL-Vinblastine COMPLEX Deposited 2015-05-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 4 CA CALCIUM ION × 3 GOL GLYCEROL × 7 GDP GUANOSINE-5'-DIPHOSPHATE × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 VLB (2ALPHA,2'BETA,3BETA,4ALPHA,5BETA)-VINCALEUKOBLASTINE × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；293 K；6% PEG, 5% glycerol, 0.1M MES, 30 mM CaCl2, 30 mM MgCl2
Resolution 2.50 Å R-free 0.235
5FNV a new complex structure of tubulin with an alpha-beta unsaturated lactone Deposited 2015-11-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 4 CA CALCIUM ION × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X3H PIRONETIN × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.7；0.1M MES PH6.7;6% PEG4000 ;5% GLYCEROL;30MM CACL2;30MM MGCL2
Resolution 2.61 Å R-free 0.226
5JQG An apo tubulin-RB-TTL complex structure used for side-by-side comparison Deposited 2016-05-04 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 4 CA CALCIUM ION × 3 CL CHLORIDE ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 6.7；293 K；0.1M MES, 6% PEG4000, 5% Glycerol, 30mM CaCl2, 30mM MgCl2
Resolution 2.24 Å R-free 0.212
5KMG Near-atomic cryo-EM structure of PRC1 bound to the microtubule Deposited 2016-06-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 42 PDB declaration: 42-meric
Chain B 1–431（431 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 14 MG MAGNESIUM ION × 14 GDP GUANOSINE-5'-DIPHOSPHATE × 14 POU Peloruside A × 14 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE；blot for 4 seconds, blot force 10
Resolution 3.50 Å
5KMG Near-atomic cryo-EM structure of PRC1 bound to the microtubule Deposited 2016-06-27 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 1–431（431 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 POU Peloruside A × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE；blot for 4 seconds, blot force 10
Resolution 3.50 Å
5KMG Near-atomic cryo-EM structure of PRC1 bound to the microtubule Deposited 2016-06-27 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 1–431（431 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 POU Peloruside A × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE；blot for 4 seconds, blot force 10
Resolution 3.50 Å
5MM4 Ustilago maydis kinesin-5 motor domain in the AMPPNP state bound to microtubules Deposited 2016-12-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 1–427（427 aa）
Not recorded MG MAGNESIUM ION × 2 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 TA1 TAXOL × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.50 Å
5MM7 Ustilago maydis kinesin-5 motor domain with N-terminal extension in the AMPPNP state bound to microtubules Deposited 2016-12-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 1–427（427 aa）
Not recorded MG MAGNESIUM ION × 2 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 TA1 TAXOL × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.10 Å
5OAM Molecular basis of human kinesin-8 function and inhibition Deposited 2017-06-23 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 1–445（445 aa）
Not recorded ZN ZINC ION × 1 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.50 Å
5OCU Molecular basis of human kinesin-8 function and inhibition Deposited 2017-07-03 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 1–445（445 aa）
Not recorded MG MAGNESIUM ION × 2 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 ZN ZINC ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.20 Å
5OGC Molecular basis of human kinesin-8 function and inhibition Deposited 2017-07-12 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 1–445（445 aa）
Not recorded 9V5 4-chloranyl-2-nitro-1-(phenylsulfonyl)benzene × 1 ZN ZINC ION × 1 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.80 Å
5SYC Near-atomic resolution cryo-EM reconstruction of peloruside-stabilized microtubule Deposited 2016-08-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 52 PDB declaration: 52-meric
Chain B 1–426（426 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 26 MG MAGNESIUM ION × 26 GDP GUANOSINE-5'-DIPHOSPHATE × 26 POU Peloruside A × 26 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE；Blot for 4 seconds, blot force 10, before plunging into liquid ethane (FEI VITROBOT MARK IV).
Resolution 3.50 Å
5SYC Near-atomic resolution cryo-EM reconstruction of peloruside-stabilized microtubule Deposited 2016-08-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 1–426（426 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 POU Peloruside A × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE；Blot for 4 seconds, blot force 10, before plunging into liquid ethane (FEI VITROBOT MARK IV).
Resolution 3.50 Å
5SYC Near-atomic resolution cryo-EM reconstruction of peloruside-stabilized microtubule Deposited 2016-08-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 1–426（426 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 POU Peloruside A × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE；Blot for 4 seconds, blot force 10, before plunging into liquid ethane (FEI VITROBOT MARK IV).
Resolution 3.50 Å
5SYE Near-atomic resolution cryo-EM reconstruction of doubly bound Taxol- and peloruside-stabilized microtubule Deposited 2016-08-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 52 PDB declaration: 156-meric
Chain B 1–426（426 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 26 MG MAGNESIUM ION × 26 GDP GUANOSINE-5'-DIPHOSPHATE × 26 TA1 TAXOL × 26 POU Peloruside A × 26 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE；Blot for 4 seconds, blot force 10, before plunging into liquid ethane (FEI VITROBOT MARK IV).
Resolution 3.50 Å
5SYE Near-atomic resolution cryo-EM reconstruction of doubly bound Taxol- and peloruside-stabilized microtubule Deposited 2016-08-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: hexameric
Chain B 1–426（426 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 POU Peloruside A × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE；Blot for 4 seconds, blot force 10, before plunging into liquid ethane (FEI VITROBOT MARK IV).
Resolution 3.50 Å
5SYE Near-atomic resolution cryo-EM reconstruction of doubly bound Taxol- and peloruside-stabilized microtubule Deposited 2016-08-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: hexameric
Chain B 1–426（426 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 POU Peloruside A × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE；Blot for 4 seconds, blot force 10, before plunging into liquid ethane (FEI VITROBOT MARK IV).
Resolution 3.50 Å
5SYF High-resolution cryo-EM reconstruction of Taxol-stabilized microtubule Deposited 2016-08-11 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 52 PDB declaration: 130-meric
Chain B 1–426（426 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 26 MG MAGNESIUM ION × 26 GDP GUANOSINE-5'-DIPHOSPHATE × 26 TA1 TAXOL × 26 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE；Blot for 4 seconds, blot force 10, before plunging into liquid ethane (FEI VITROBOT MARK IV).
Resolution 3.50 Å
5SYF High-resolution cryo-EM reconstruction of Taxol-stabilized microtubule Deposited 2016-08-11 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: pentameric
Chain B 1–426（426 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE；Blot for 4 seconds, blot force 10, before plunging into liquid ethane (FEI VITROBOT MARK IV).
Resolution 3.50 Å
5SYF High-resolution cryo-EM reconstruction of Taxol-stabilized microtubule Deposited 2016-08-11 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: pentameric
Chain B 1–426（426 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE；Blot for 4 seconds, blot force 10, before plunging into liquid ethane (FEI VITROBOT MARK IV).
Resolution 3.50 Å
5SYG Cryo-EM reconstruction of zampanolide-bound microtubule Deposited 2016-08-11 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 52 PDB declaration: 130-meric
Chain B 1–426（426 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 26 MG MAGNESIUM ION × 26 GDP GUANOSINE-5'-DIPHOSPHATE × 26 ZPN (2Z,4E)-N-[(S)-[(1S,2E,5S,8E,10Z,17S)-3,11-dimethyl-19-methylidene-7,13-dioxo-6,21-dioxabicyclo[15.3.1]henicosa-2,8,10-trien-5-yl](hydroxy)methyl]hexa-2,4-dienamide × 26 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE；Blot for 4 seconds, blot force 10, before plunging into liquid ethane (FEI VITROBOT MARK IV).
Resolution 3.50 Å
5SYG Cryo-EM reconstruction of zampanolide-bound microtubule Deposited 2016-08-11 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: pentameric
Chain B 1–426（426 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ZPN (2Z,4E)-N-[(S)-[(1S,2E,5S,8E,10Z,17S)-3,11-dimethyl-19-methylidene-7,13-dioxo-6,21-dioxabicyclo[15.3.1]henicosa-2,8,10-trien-5-yl](hydroxy)methyl]hexa-2,4-dienamide × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE；Blot for 4 seconds, blot force 10, before plunging into liquid ethane (FEI VITROBOT MARK IV).
Resolution 3.50 Å
5SYG Cryo-EM reconstruction of zampanolide-bound microtubule Deposited 2016-08-11 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: pentameric
Chain B 1–426（426 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ZPN (2Z,4E)-N-[(S)-[(1S,2E,5S,8E,10Z,17S)-3,11-dimethyl-19-methylidene-7,13-dioxo-6,21-dioxabicyclo[15.3.1]henicosa-2,8,10-trien-5-yl](hydroxy)methyl]hexa-2,4-dienamide × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE；Blot for 4 seconds, blot force 10, before plunging into liquid ethane (FEI VITROBOT MARK IV).
Resolution 3.50 Å
5XXT GDP-microtubule complexed with nucleotide-free KIF5C Deposited 2017-07-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain B 2–427（426 aa） Fragment：UNP residues 2-427
Chain D 2–427（426 aa） Fragment：UNP residues 2-427
Chain F 2–427（426 aa） Fragment：UNP residues 2-427
Chain H 2–427（426 aa） Fragment：UNP residues 2-427
Chain J 2–427（426 aa） Fragment：UNP residues 2-427
Chain L 2–427（426 aa） Fragment：UNP residues 2-427
Chain N 2–427（426 aa） Fragment：UNP residues 2-427
Chain P 2–427（426 aa） Fragment：UNP residues 2-427
Chain R 2–427（426 aa） Fragment：UNP residues 2-427
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 9 MG MAGNESIUM ION × 9 GDP GUANOSINE-5'-DIPHOSPHATE × 9 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.35 Å
5XXV GDP-microtubule complexed with KIF5C in AMPPNP state Deposited 2017-07-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain B 2–427（426 aa） Fragment：UNP residues 2-427
Chain D 2–427（426 aa） Fragment：UNP residues 2-427
Chain F 2–427（426 aa） Fragment：UNP residues 2-427
Chain H 2–427（426 aa） Fragment：UNP residues 2-427
Chain J 2–427（426 aa） Fragment：UNP residues 2-427
Chain L 2–427（426 aa） Fragment：UNP residues 2-427
Chain N 2–427（426 aa） Fragment：UNP residues 2-427
Chain P 2–427（426 aa） Fragment：UNP residues 2-427
Chain R 2–427（426 aa） Fragment：UNP residues 2-427
Not recorded MG MAGNESIUM ION × 9 GTP GUANOSINE-5'-TRIPHOSPHATE × 9 GDP GUANOSINE-5'-DIPHOSPHATE × 9 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.46 Å
5XXW GDP-microtubule complexed with KIF5C in ATP state Deposited 2017-07-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain B 2–427（426 aa） Fragment：UNP residues 2-427
Chain D 2–427（426 aa） Fragment：UNP residues 2-427
Chain F 2–427（426 aa） Fragment：UNP residues 2-427
Chain H 2–427（426 aa） Fragment：UNP residues 2-427
Chain J 2–427（426 aa） Fragment：UNP residues 2-427
Chain L 2–427（426 aa） Fragment：UNP residues 2-427
Chain N 2–427（426 aa） Fragment：UNP residues 2-427
Chain P 2–427（426 aa） Fragment：UNP residues 2-427
Chain R 2–427（426 aa） Fragment：UNP residues 2-427
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 9 MG MAGNESIUM ION × 9 GDP GUANOSINE-5'-DIPHOSPHATE × 9 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.00 Å
5XXX GMPCPP-microtubule complexed with nucleotide-free KIF5C Deposited 2017-07-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric
Chain B 2–427（426 aa） Fragment：UNP residues 2-427
Chain D 2–427（426 aa） Fragment：UNP residues 2-427
Chain F 2–427（426 aa） Fragment：UNP residues 2-427
Chain H 2–427（426 aa） Fragment：UNP residues 2-427
Chain J 2–427（426 aa） Fragment：UNP residues 2-427
Chain L 2–427（426 aa） Fragment：UNP residues 2-427
Chain N 2–427（426 aa） Fragment：UNP residues 2-427
Chain P 2–427（426 aa） Fragment：UNP residues 2-427
Chain R 2–427（426 aa） Fragment：UNP residues 2-427
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 9 MG MAGNESIUM ION × 18 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 9 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.43 Å
6BJC TPX2_mini decorated GMPCPP-microtubule Deposited 2017-11-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 14 PDB declaration: tetradecameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Chain F 1–445（445 aa）
Chain G 1–445（445 aa）
Chain H 1–445（445 aa）
Chain I 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 12 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8；BRB80
cryo-EM vitrification conditions Cryogen ETHANE；blot for 4 seconds before plunging
Resolution 3.30 Å
6CVJ Model of synthetic tau (four tandem repeats of first repeat sequence) bound to the microtubule Deposited 2018-03-28 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 120 PDB declaration: 120-meric
Chain B 1–445（445 aa）
Chain C 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 30 MG MAGNESIUM ION × 30 GDP GUANOSINE-5'-DIPHOSPHATE × 60 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE；blot force 10 pN, 6 second blot time. used C-flat 1.2/1.3 holey grids. First 2 uL of microtubules were adhered to grid for 30 seconds, followed by 2 4 uL washes of tau with 30 second incubation for each wash
Resolution 3.20 Å
6CVJ Model of synthetic tau (four tandem repeats of first repeat sequence) bound to the microtubule Deposited 2018-03-28 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain B 1–445（445 aa）
Chain C 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE；blot force 10 pN, 6 second blot time. used C-flat 1.2/1.3 holey grids. First 2 uL of microtubules were adhered to grid for 30 seconds, followed by 2 4 uL washes of tau with 30 second incubation for each wash
Resolution 3.20 Å
6CVJ Model of synthetic tau (four tandem repeats of first repeat sequence) bound to the microtubule Deposited 2018-03-28 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain B 1–445（445 aa）
Chain C 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE；blot force 10 pN, 6 second blot time. used C-flat 1.2/1.3 holey grids. First 2 uL of microtubules were adhered to grid for 30 seconds, followed by 2 4 uL washes of tau with 30 second incubation for each wash
Resolution 3.20 Å
6CVN Model of synthetic tau (R2x4) bound to the microtubule Deposited 2018-03-28 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 120 PDB declaration: 120-meric
Chain A 1–445（445 aa）
Chain C 1–445（445 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 60 GTP GUANOSINE-5'-TRIPHOSPHATE × 30 MG MAGNESIUM ION × 30 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE；blot force 10 pN, 6 second blot time. used C-flat 1.2/1.3 holey grids. First 2 uL of microtubules were adhered to grid for 30 seconds, followed by 2 4 uL washes of tau with 30 second incubation for each wash.
Resolution 3.90 Å
6CVN Model of synthetic tau (R2x4) bound to the microtubule Deposited 2018-03-28 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain A 1–445（445 aa）
Chain C 1–445（445 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE；blot force 10 pN, 6 second blot time. used C-flat 1.2/1.3 holey grids. First 2 uL of microtubules were adhered to grid for 30 seconds, followed by 2 4 uL washes of tau with 30 second incubation for each wash.
Resolution 3.90 Å
6CVN Model of synthetic tau (R2x4) bound to the microtubule Deposited 2018-03-28 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain A 1–445（445 aa）
Chain C 1–445（445 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE；blot force 10 pN, 6 second blot time. used C-flat 1.2/1.3 holey grids. First 2 uL of microtubules were adhered to grid for 30 seconds, followed by 2 4 uL washes of tau with 30 second incubation for each wash.
Resolution 3.90 Å
6DPU Undecorated GMPCPP microtubule Deposited 2018-06-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Chain F 1–445（445 aa）
Chain G 1–445（445 aa）
Chain H 1–445（445 aa）
Chain I 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 12 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8；BRB80 buffer (80 mM PIPES, pH 6.8, 1 mM EGTA, 1 mM MgCl2) supplemented with 1 mM GMPCPP, 1 mM DTT, and 0.05% Nonident P-40
cryo-EM vitrification conditions Cryogen ETHANE；Blot for 4 seconds before plunging.
Resolution 3.10 Å
6DPV Undecorated GDP microtubule Deposited 2018-06-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Chain F 1–445（445 aa）
Chain G 1–445（445 aa）
Chain H 1–445（445 aa）
Chain I 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8；BRB80 buffer (80 mM PIPES pH 6.8, 1 mM EGTA, 1 mM MgCl2) supplemented with 1 mM GTP, 1 mM DTT, and 0.05% Nonident P-40
cryo-EM vitrification conditions Cryogen ETHANE；Blot for 4 seconds before plunging.
Resolution 3.30 Å
6DPW Undecorated GTPgammaS microtubule Deposited 2018-06-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Chain F 1–445（445 aa）
Chain G 1–445（445 aa）
Chain H 1–445（445 aa）
Chain I 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 6 GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8；Modified BRB80 buffer (80 mM PIPES, pH 6.8, 1 mM EGTA, 0.2 mM MgCl2) supplemented with 1 mM GTPgammaS, 1 mM DTT, and 0.05% Nonident P-40
cryo-EM vitrification conditions Cryogen ETHANE；Blot for 4 seconds before plunging.
Resolution 3.50 Å
6EVW Cryo-EM structure of GMPCPP-microtubule co-polymerised with doublecortin Deposited 2017-11-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain B 1–429（429 aa）
Chain D 1–429（429 aa）
Chain F 1–429（429 aa）
Chain G 1–429（429 aa）
Chain H 1–429（429 aa）
Chain I 1–429（429 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 12 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.40 Å
6EVX Cryo-EM structure of GDP.Pi-microtubule rapidly co-polymerised with doublecortin Deposited 2017-11-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Chain F 1–445（445 aa）
Chain G 1–445（445 aa）
Chain H 1–445（445 aa）
Chain I 1–445（445 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 6 PO4 PHOSPHATE ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
6EVY Cryo-EM structure of GTPgammaS-microtubule co-polymerised with doublecortin Deposited 2017-11-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Chain F 1–445（445 aa）
Chain G 1–445（445 aa）
Chain H 1–445（445 aa）
Chain I 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 6 GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.40 Å
6EVZ Cryo-EM structure of GDP-microtubule co-polymerised with doublecortin Deposited 2017-11-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Chain F 1–445（445 aa）
Chain G 1–445（445 aa）
Chain H 1–445（445 aa）
Chain I 1–445（445 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
6EW0 Cryo-EM structure of GDP-microtubule co-polymerised with doublecortin and supplemented with Taxol Deposited 2017-11-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Chain F 1–445（445 aa）
Chain G 1–445（445 aa）
Chain H 1–445（445 aa）
Chain I 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 6 TA1 TAXOL × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
6KIO Complex of yeast cytoplasmic dynein MTBD-High and MT without DTT Deposited 2019-07-19 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain b 2–427（426 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.94 Å
6KIQ Complex of yeast cytoplasmic dynein MTBD-High and MT with DTT Deposited 2019-07-19 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain b 2–427（426 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8；DTT was added to the final concentration of 1 mM.
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.62 Å
6LS4 A novel anti-tumor agent S-40 in complex with tubulin Deposited 2020-01-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 GOL GLYCEROL × 1 S40 3-[(4-cyclopropylphenyl)sulfonylamino]-4-methyl-N-(pyridin-3-ylmethyl)benzamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；291 K；6% PEG 4000, 6% GLYCEROL, 30mM MAGNESIUM CHLORIDE, 30mM CALCIUM CHLORIDE, 3.3% Jeffamin M-600, 100mM MES
Resolution 2.40 Å R-free 0.240
6MLQ Cryo-EM structure of microtubule-bound Kif7 in the ADP state Deposited 2018-09-27 Different ligand/ion Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 1–445（445 aa）
Not recorded MG MAGNESIUM ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE；Blotted from behind the grid for 2 seconds
Resolution 4.20 Å
6MZE Structural Basis of Tubulin Recruitment and Assembly by Microtubule Polymerases with Tumor Overexpressed Gene (TOG) Domain Arrays Deposited 2018-11-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 14 PDB declaration: tetradecameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Chain I 1–445（445 aa）
Chain K 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；50 mM PIPES, 100 mM MgCl2 [pH 7.0], and 10-15% PEG-8000
Resolution 3.60 Å R-free 0.245
6MZE Structural Basis of Tubulin Recruitment and Assembly by Microtubule Polymerases with Tumor Overexpressed Gene (TOG) Domain Arrays Deposited 2018-11-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 14 PDB declaration: tetradecameric
Chain P 1–445（445 aa）
Chain R 1–445（445 aa）
Chain W 1–445（445 aa）
Chain Y 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；50 mM PIPES, 100 mM MgCl2 [pH 7.0], and 10-15% PEG-8000
Resolution 3.60 Å R-free 0.245
6MZF Structural Basis of Tubulin Recruitment and Assembly by Microtubule Polymerases with Tumor Overexpressed Gene (TOG) Domain Arrays Deposited 2018-11-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 14 PDB declaration: tetradecameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Chain I 1–445（445 aa）
Chain K 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；50 mM PIPES, 100 mM MgCl2 [pH 7.0], and 10-15% PEG-8000
Resolution 4.40 Å R-free 0.252
6MZF Structural Basis of Tubulin Recruitment and Assembly by Microtubule Polymerases with Tumor Overexpressed Gene (TOG) Domain Arrays Deposited 2018-11-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 14 PDB declaration: tetradecameric
Chain P 1–445（445 aa）
Chain R 1–445（445 aa）
Chain W 1–445（445 aa）
Chain Y 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；50 mM PIPES, 100 mM MgCl2 [pH 7.0], and 10-15% PEG-8000
Resolution 4.40 Å R-free 0.252
6MZG Structural Basis of Tubulin Recruitment and Assembly by Microtubule Polymerases with Tumor Overexpressed Gene (TOG) Domain Arrays Deposited 2018-11-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 4 GDP GUANOSINE-5'-DIPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；50 mM PIPES, 100 mM MgCl2 [pH 7.0], and 10-15% PEG-8000
Resolution 3.21 Å R-free 0.234
6MZG Structural Basis of Tubulin Recruitment and Assembly by Microtubule Polymerases with Tumor Overexpressed Gene (TOG) Domain Arrays Deposited 2018-11-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain H 1–445（445 aa）
Chain J 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 4 GDP GUANOSINE-5'-DIPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；50 mM PIPES, 100 mM MgCl2 [pH 7.0], and 10-15% PEG-8000
Resolution 3.21 Å R-free 0.234
6O2Q Acetylated Microtubules Deposited 2019-02-24 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Chain F 1–445（445 aa）
Chain G 1–445（445 aa）
Chain H 1–445（445 aa）
Chain I 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8；Contains 80 mM PIPES, 1 mM MgCl2, 1 mM EGTA, pH 6.8 with KOH (stored at 4 degrees Celsius).
cryo-EM vitrification conditions Cryogen ETHANE；Blotted for 4 seconds at blot force 10.
Resolution 3.70 Å
6O2R Deacetylated Microtubules Deposited 2019-02-24 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Chain F 1–445（445 aa）
Chain G 1–445（445 aa）
Chain H 1–445（445 aa）
Chain I 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8；Contains 80 mM PIPES, 1 mM MgCl2, 1 mM EGTA, pH 6.8 with KOH (stored at 4 degrees Celsius).
cryo-EM vitrification conditions Cryogen ETHANE；Blotted for 4 seconds at blot force 10.
Resolution 3.30 Å
6O2S Deacetylated Microtubules Deposited 2019-02-24 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 104 PDB declaration: 104-meric
Chain 1H 1–445（445 aa）
Chain 1O 1–445（445 aa）
Chain 1P 1–445（445 aa）
Chain 1Q 1–445（445 aa）
Chain 1R 1–445（445 aa）
Chain 1S 1–445（445 aa）
Chain 1T 1–445（445 aa）
Chain 1U 1–445（445 aa）
Chain 1V 1–445（445 aa）
Chain 1W 1–445（445 aa）
Chain 1X 1–445（445 aa）
Chain 1Y 1–445（445 aa）
Chain 1Z 1–445（445 aa）
Chain 2H 1–445（445 aa）
Chain 2O 1–445（445 aa）
Chain 2P 1–445（445 aa）
Chain 2Q 1–445（445 aa）
Chain 2R 1–445（445 aa）
Chain 2S 1–445（445 aa）
Chain 2T 1–445（445 aa）
Chain 2U 1–445（445 aa）
Chain 2V 1–445（445 aa）
Chain 2W 1–445（445 aa）
Chain 2X 1–445（445 aa）
Chain 2Y 1–445（445 aa）
Chain 2Z 1–445（445 aa）
Chain 3H 1–445（445 aa）
Chain 3O 1–445（445 aa）
Chain 3P 1–445（445 aa）
Chain 3Q 1–445（445 aa）
Chain 3R 1–445（445 aa）
Chain 3S 1–445（445 aa）
Chain 3T 1–445（445 aa）
Chain 3U 1–445（445 aa）
Chain 3V 1–445（445 aa）
Chain 3W 1–445（445 aa）
Chain 3X 1–445（445 aa）
Chain 3Y 1–445（445 aa）
Chain 3Z 1–445（445 aa）
Chain 4H 1–445（445 aa）
Chain 4O 1–445（445 aa）
Chain 4P 1–445（445 aa）
Chain 4Q 1–445（445 aa）
Chain 4R 1–445（445 aa）
Chain 4S 1–445（445 aa）
Chain 4T 1–445（445 aa）
Chain 4U 1–445（445 aa）
Chain 4V 1–445（445 aa）
Chain 4W 1–445（445 aa）
Chain 4X 1–445（445 aa）
Chain 4Y 1–445（445 aa）
Chain 4Z 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 52 MG MAGNESIUM ION × 52 GDP GUANOSINE-5'-DIPHOSPHATE × 52 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8；Contains 80 mM PIPES, 1 mM MgCl2, 1 mM EGTA, pH 6.8 with KOH (stored at 4 degrees Celsius).
cryo-EM vitrification conditions Cryogen ETHANE；Blotted for 4 seconds at blot force 10.
Resolution 4.00 Å
6O2T Acetylated Microtubules Deposited 2019-02-24 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 104 PDB declaration: 104-meric
Chain 1H 1–445（445 aa）
Chain 1O 1–445（445 aa）
Chain 1P 1–445（445 aa）
Chain 1Q 1–445（445 aa）
Chain 1R 1–445（445 aa）
Chain 1S 1–445（445 aa）
Chain 1T 1–445（445 aa）
Chain 1U 1–445（445 aa）
Chain 1V 1–445（445 aa）
Chain 1W 1–445（445 aa）
Chain 1X 1–445（445 aa）
Chain 1Y 1–445（445 aa）
Chain 1Z 1–445（445 aa）
Chain 2H 1–445（445 aa）
Chain 2O 1–445（445 aa）
Chain 2P 1–445（445 aa）
Chain 2Q 1–445（445 aa）
Chain 2R 1–445（445 aa）
Chain 2S 1–445（445 aa）
Chain 2T 1–445（445 aa）
Chain 2U 1–445（445 aa）
Chain 2V 1–445（445 aa）
Chain 2W 1–445（445 aa）
Chain 2X 1–445（445 aa）
Chain 2Y 1–445（445 aa）
Chain 2Z 1–445（445 aa）
Chain 3H 1–445（445 aa）
Chain 3O 1–445（445 aa）
Chain 3P 1–445（445 aa）
Chain 3Q 1–445（445 aa）
Chain 3R 1–445（445 aa）
Chain 3S 1–445（445 aa）
Chain 3T 1–445（445 aa）
Chain 3U 1–445（445 aa）
Chain 3V 1–445（445 aa）
Chain 3W 1–445（445 aa）
Chain 3X 1–445（445 aa）
Chain 3Y 1–445（445 aa）
Chain 3Z 1–445（445 aa）
Chain 4H 1–445（445 aa）
Chain 4O 1–445（445 aa）
Chain 4P 1–445（445 aa）
Chain 4Q 1–445（445 aa）
Chain 4R 1–445（445 aa）
Chain 4S 1–445（445 aa）
Chain 4T 1–445（445 aa）
Chain 4U 1–445（445 aa）
Chain 4V 1–445（445 aa）
Chain 4W 1–445（445 aa）
Chain 4X 1–445（445 aa）
Chain 4Y 1–445（445 aa）
Chain 4Z 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 52 MG MAGNESIUM ION × 52 GDP GUANOSINE-5'-DIPHOSPHATE × 52 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8；Contains 80 mM PIPES, 1 mM MgCl2, 1 mM EGTA, pH 6.8 with KOH (stored at 4 degrees Celsius).
cryo-EM vitrification conditions Cryogen ETHANE；Blotted for 4 seconds at blot force 10.
Resolution 4.10 Å
6RZA Cryo-EM structure of the human inner arm dynein DNAH7 microtubule binding domain bound to microtubules Deposited 2019-06-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 5 PDB declaration: pentameric
Chain B 1–426（426 aa）
Chain D 1–426（426 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 TA1 TAXOL × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.50 Å
6RZB Cryo-EM structure of mouse cytoplasmic dynein-1 microtubule binding domain bound to microtubules Deposited 2019-06-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 1–426（426 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
6TA3 Human kinesin-5 motor domain in the GSK-1 state bound to microtubules (Conformation 1) Deposited 2019-10-29 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 1–429（429 aa）
Not recorded G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 2 MG MAGNESIUM ION × 2 MZK 6-[4-(trifluoromethyl)phenyl]-3,4-dihydro-1~{H}-quinolin-2-one × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
6TA4 Human kinesin-5 motor domain in the AMPPNP state bound to microtubules Deposited 2019-10-29 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 1–429（429 aa）
Not recorded G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.2
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.10 Å
6TIW Human kinesin-5 motor domain in the GSK state bound to microtubules (Conformation 2) Deposited 2019-11-22 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 1–429（429 aa）
Not recorded MZK 6-[4-(trifluoromethyl)phenyl]-3,4-dihydro-1~{H}-quinolin-2-one × 1 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 2 MG MAGNESIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
6VPO Cryo-EM structure of microtubule-bound KLP61F motor domain in the AMPPNP state Deposited 2020-02-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.40 Å
6VPP Cryo-EM structure of microtubule-bound KLP61F motor with tail domain in the nucleotide-free state Deposited 2020-02-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.40 Å
6Y4M Structure of Tubulin Tyrosine Ligase in Complex with Tb111 Deposited 2020-02-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 4 CA CALCIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 O9B (2~{R})-1-methylpiperidine-2-carboxylic acid × 1 O9H [(1~{R},3~{R})-1-acetyloxy-1-[4-methanoyl-5-(2-phenylmethoxyethyl)-2,3-dihydro-1,3-thiazol-2-yl]-4-methyl-pentan-3-yl]-methyl-azanium × 1 OH5 (2~{S},4~{R})-4-azanyl-2-methyl-5-phenyl-pentanoic acid × 1 PGE TRIETHYLENE GLYCOL × 1 VAL VALINE × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；0.03 M calcium chloride, 0.03 M magnesium chloride, 0.10 M MES pH 6.50, 3.20% w/v PEG 4000, 5.00% w/v glycerol
Resolution 3.34 Å R-free 0.234
6Y4N Structure of Tubulin Tyrosine Ligase in Complex with Tb116 Deposited 2020-02-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 5 CA CALCIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 O9B (2~{R})-1-methylpiperidine-2-carboxylic acid × 1 O9K [(1~{R},3~{R})-1-(4-methanoyl-1,3-thiazol-2-yl)-4-methyl-3-(methylamino)pentyl] ethanoate × 1 O9N methyl (2~{S},4~{S})-2,4-bis(azanyl)-5-phenyl-pentanoate × 1 PGE TRIETHYLENE GLYCOL × 1 VAL VALINE × 1 P6S benzyl hydrogen carbonate × 1 PEG DI(HYDROXYETHYL)ETHER × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；293 K；30 mM calcium chloride, 30 mM magnesium chloride, 0.10 M MES pH 6.0, 2.0% w/v PEG 4000, 5.0% w/v glycerol
Resolution 2.85 Å R-free 0.220
6ZPI Microtubule complexed with Kif15 motor domain. Symmetrised asymmetric unit Deposited 2020-07-08 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 1–431（431 aa）
Not recorded ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 MG MAGNESIUM ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.50 Å
7CBZ Crystal structure of T2R-TTL-A31 complex Deposited 2020-06-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 5 CA CALCIUM ION × 6 FUO 2-[5-[4-[2-[4-(2-cyclopropylethanoyl)piperazin-1-yl]ethoxy]phenyl]pyridin-2-yl]-N-(phenylmethyl)ethanamide × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；6% PEG4000, 8% glycerol, 0.1 M MES (pH 6.7), 30 mM CaCl2, and 30 mM MgCl2.
Resolution 2.61 Å R-free 0.255
7DMZ GMPCPP microtubule complex Deposited 2020-12-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain C 1–445（445 aa）
Chain E 1–445（445 aa）
Chain F 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 2 TAJ taccalonolide AJ × 3 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
7DN0 AJ-GMPCPP-MT-non-seam Deposited 2020-12-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain E 1–445（445 aa）
Chain F 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 4 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 2 TAJ taccalonolide AJ × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
7EMJ Crystal structure of T2R-TTL-Barbigerone complex Deposited 2021-04-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 5 CA CALCIUM ION × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 J6L 8,8-dimethyl-3-(2,4,5-trimethoxyphenyl)pyrano[2,3-f]chromen-4-one × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；6% PEG 4000, 5% glycerol, 0.1 M MES, 30 mM CaCl2 and 30 mM MgCl2, pH 6.7
Resolution 2.33 Å R-free 0.241
7EXC Crystal structure of T2R-TTL-1129A2 complex Deposited 2021-05-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 4 CA CALCIUM ION × 2 GOL GLYCEROL × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 JEL N-[[3-(1,3-benzodioxol-5-yloxy)phenyl]methyl]-9H-pyrido[3,4-b]indol-3-amine × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；6% poly(ethylene glycol) 4000, 8% glycerol, 0.1 M MES (pH 6.7), 30 mM CaCl2, 30 mM MgCl2
Resolution 2.39 Å R-free 0.264
7L05 Complex of novel maytansinoid M24 bound to T2R-TTL (two tubulin alpha/beta heterodimers, RB3 stathmin-like domain, and tubulin tyrosine ligase) Deposited 2020-12-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 5 CA CALCIUM ION × 4 CL CHLORIDE ION × 1 EDO 1,2-ETHANEDIOL × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 XQ4 (1S,2R,3S,5S,6S,16E,18E,20R,21S)-11-chloro-21-hydroxy-12,20-dimethoxy-2,5,9,16-tetramethyl-8,23-dioxo-4,24-dioxa-9,22-diazatetracyclo[19.3.1.1~10,14~.0~3,5~]hexacosa-10(26),11,13,16,18-pentaen-6-yl (2S)-2-{methyl[3-(methylamino)propanoyl]amino}propanoate (non-preferred name) × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.5；298 K；PEG 3350, MES, CaCl2, MgCl2, glycerol
Resolution 2.21 Å R-free 0.217
7NB8 Plasmodium falciparum kinesin-5 motor domain without nucleotide, complexed with 14 protofilament microtubule. Deposited 2021-01-25 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 2 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；3.5 uM microtubules were incubated to the grid and incubated for 30 seconds. The grid was blotted, then 40 uM kinesin motor domain added and incubated for 30 seconds. The grid was again blotted and 40 uM kinesin motor domain added for 30 seconds. Then the grid was blotted and plunge frozen.
Resolution 4.40 Å
7NBA Plasmodium falciparum kinesin-5 motor domain bound to AMPPNP, complexed with 14 protofilament microtubule. Deposited 2021-01-25 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 3 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；5 uM microtubules were applied to the grid and incubated for 30 seconds. The grid was blotted, then 40 uM kinesin motor domain added, incubated for 30 seconds, then blotted. 40 uM Kinesin motor domain was again added, incubated for 40 seconds, blotted, then plunge frozen.
Resolution 4.00 Å
7PQC tau-microtubule structural ensemble based on CryoEM data Deposited 2021-09-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric
Chain A 1–445（445 aa）
Chain C 1–445（445 aa）
Chain E 1–445（445 aa）
Chain G 1–445（445 aa）
Chain I 1–445（445 aa）
Chain K 1–445（445 aa）
Chain M 1–445（445 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 7 MG MAGNESIUM ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
7PQP tau-microtubule structural ensemble based on CryoEM data Deposited 2021-09-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric
Chain A 1–445（445 aa）
Chain C 1–445（445 aa）
Chain E 1–445（445 aa）
Chain G 1–445（445 aa）
Chain I 1–445（445 aa）
Chain K 1–445（445 aa）
Chain M 1–445（445 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 7 MG MAGNESIUM ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
7RS5 Cryo-EM structure of Kip3 (AMPPNP) bound to Taxol-Stabilized Microtubules Deposited 2021-08-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 27 PDB declaration: 27-meric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Chain F 1–445（445 aa）
Chain H 1–445（445 aa）
Chain J 1–445（445 aa）
Chain M 1–445（445 aa）
Chain O 1–445（445 aa）
Chain Q 1–445（445 aa）
Chain S 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 9 MG MAGNESIUM ION × 18 GDP GUANOSINE-5'-DIPHOSPHATE × 9 TA1 TAXOL × 9 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 9 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；cryoEM buffer (50 mM Tris-HCl, pH 8.0, 1 mM MgCl2, 1 mM EGTA, 1 mM DTT supplemented with 2mM AMPPNP)
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
7RS6 Cryo-EM structure of Kip3 (AMPPNP) bound to GMPCPP-Stabilized Microtubules Deposited 2021-08-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 27 PDB declaration: 27-meric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Chain F 1–445（445 aa）
Chain H 1–445（445 aa）
Chain J 1–445（445 aa）
Chain M 1–445（445 aa）
Chain O 1–445（445 aa）
Chain Q 1–445（445 aa）
Chain S 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 9 MG MAGNESIUM ION × 27 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 9 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 9 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8；BRB80 buffer (80 mM PIPES-KOH, pH 6.8; 1 mM MgCl2, 1 mM EGTA, 1 mM DTT)
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
7RX0 Complex of AMPPNP-Kif7 and Gli2 Zinc-Finger domain bound to microtubules Deposited 2021-08-20 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain B 1–445（445 aa）
Not recorded ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE；Blotted from behind the grid for 2 seconds
Resolution 3.89 Å
7SGS Cryo-EM structure of full-length MAP7 bound to the microtubule Deposited 2021-10-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain C 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
7U0F HIV-1 Rev in complex with tubulin Deposited 2022-02-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 150 PDB declaration: 150-meric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.53 Å
7U0F HIV-1 Rev in complex with tubulin Deposited 2022-02-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.53 Å
7U0F HIV-1 Rev in complex with tubulin Deposited 2022-02-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.53 Å
7X4N Crystal Structure of C. elegans kinesin-4 KLP-12 complexed with tubulin and DARPin Deposited 2022-03-03 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain B 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；ammonium acetate, HEPES, polyethylene glycol (PEG) 3350
Resolution 2.88 Å R-free 0.296
7YHN ANTI-TUMOR AGENT Y48 IN COMPLEX WITH TUBULIN Deposited 2022-07-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 IUK 4-methyl-3-[(4-methylphenyl)sulfonylamino]-~{N}-[(6-methylpyridin-3-yl)methyl]benzamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；291 K；6% PEG 4000, 8% GLYCEROL, 30MM MAGNESIUM CHLORIDE, 30MM CALCIUM CHLORIDE, 100MM MES
Resolution 2.60 Å R-free 0.272
7YSN Tubulin heterodimer structure of GMPCPP state in solution Deposited 2022-08-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
7YSO Tubulin heterodimer structure of GDP-1 state in solution Deposited 2022-08-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
7YSP Tubulin heterodimer structure of GDP-2 state in solution Deposited 2022-08-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
7Z2A P. berghei kinesin-8B motor domain in no nucleotide state bound to tubulin dimer Deposited 2022-02-26 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain H 1–426（426 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 2 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
7Z2B P. berghei kinesin-8B motor domain in AMPPNP state bound to tubulin dimer Deposited 2022-02-26 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain H 1–426（426 aa）
Not recorded ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 MG MAGNESIUM ION × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
7Z2C P. falciparum kinesin-8B motor domain in no nucleotide bound to tubulin dimer Deposited 2022-02-26 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain H 1–426（426 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 2 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
8JJB Crystal structure of T2R-TTL-Y61 complex Deposited 2023-05-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain B 1–431（431 aa）
Chain D 1–431（431 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 5 CA CALCIUM ION × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 USI ~{N}4-(1~{H}-indol-5-ylmethyl)-6-(3-methoxyphenyl)pyrimidine-2,4-diamine × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 CL CHLORIDE ION × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；6% poly(ethylene glycol) 4000, 8% glycerol, 0.1 M MES (pH 6.7), 30 mM CaCl2, 30 mM MgCl2
Resolution 2.68 Å R-free 0.260
8JJC Tubulin-Y62 Deposited 2023-05-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain B 1–431（431 aa）
Chain D 1–431（431 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 5 CA CALCIUM ION × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 UPO 4-(6,7-dimethoxy-3,4-dihydro-1~{H}-isoquinolin-2-yl)-6-(3-methoxyphenyl)pyrimidin-2-amine × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 CL CHLORIDE ION × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；6% poly(ethylene glycol) 4000, 8% glycerol, 0.1 M MES (pH 6.7), 30 mM CaCl2, 30 mM MgCl2
Resolution 2.76 Å R-free 0.264
8QAU Outer kinetochore Ndc80-Dam1 alpha/beta-tubulin complex Deposited 2023-08-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain D 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.54 Å
8RC1 MAP7 MTBD (microtubule binding domain) decorated microtubule protofilament Deposited 2023-12-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain B 1–430（430 aa）
Chain D 1–430（430 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.5；80mM PIPES, 2mM MgCl2, 1mM EGTA
cryo-EM vitrification conditions Cryogen ETHANE；Microtubules stabilised by MAP7 MTBD were applied to a glow discharged grid, incubated for 30 seconds at room temperature following which excess sample was wicked off and MAP7 MTBD was immediately added to the grid. After incubation for 60 seconds in a Vitrobot, excess liquid was blotted off and the grid was plunge frozen in liquid ethane.
Resolution 3.70 Å
8RHB Microtubule-associated kinesin-1 tail complex bound to AMPPNP, single-headed state Deposited 2023-12-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain B 1–445（445 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 MG MAGNESIUM ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8；BRB80 + 5mM AMPPNP
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RHH Microtubule-associated kinesin-1 tail complex bound to AMPPNP, two-headed state Deposited 2023-12-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: pentameric
Chain B 1–445（445 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 2 MG MAGNESIUM ION × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8；BRB80 + 5mM AMPPNP
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8RIK Microtubule-associated kinesin-1 tail complex bound to ADP, single-headed state Deposited 2023-12-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: tetrameric
Chain B 1–445（445 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8；BRB80 + 5 mM ADP
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8RIZ Microtubule-associated kinesin-1 tail complex bound to ADP, two-headed state Deposited 2023-12-19 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain B 1–445（445 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8；BRB80 + 5mM ADP
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8V4K CCP5 in complex with microtubules class1 Deposited 2023-11-29 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Not recorded MG MAGNESIUM ION × 4 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 2 ZN ZINC ION × 1 GLU GLUTAMIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8V4L CCP5 in complex with microtubules class2 Deposited 2023-11-29 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: hexameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Not recorded MG MAGNESIUM ION × 4 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 2 GLU GLUTAMIC ACID × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8V4M CCP5 in complex with microtubules class3 Deposited 2023-11-29 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: hexameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Not recorded MG MAGNESIUM ION × 4 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 2 GLU GLUTAMIC ACID × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8X9P HURP (428-534)-alpha-tubulin-beta-tubulin complex Deposited 2023-11-30 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 1–427（427 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.54 Å
9DA8 Tau-Microtubule structure in the presence of ATP Deposited 2024-08-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–445（445 aa）
Chain C 1–445（445 aa）
Chain K 1–445（445 aa）
Chain M 1–445（445 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 4 TA1 TAXOL × 4 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.94 Å
9DHZ Cryo-EM structure of HURP bound to a microtubule Deposited 2024-09-04 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain A 1–445（445 aa）
Chain K 1–445（445 aa）
Not recorded TA1 TAXOL × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9DI0 Cryo-EM structure of Kif18A bound to a microtubule Deposited 2024-09-04 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 3 PDB declaration: trimeric
Chain K 1–445（445 aa）
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 2 TA1 TAXOL × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9DUQ HURP(65-174) bound to GMPCPP-stabilized microtubule Deposited 2024-10-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 27 PDB declaration: 27-meric
Chain B 1–427（427 aa）
Chain D 1–427（427 aa）
Chain F 1–427（427 aa）
Chain H 1–427（427 aa）
Chain J 1–427（427 aa）
Chain L 1–427（427 aa）
Chain N 1–427（427 aa）
Chain P 1–427（427 aa）
Chain R 1–427（427 aa）
Not recorded G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 9 MG MAGNESIUM ION × 18 GTP GUANOSINE-5'-TRIPHOSPHATE × 9 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8；BRB80 (1X): 80 mM PIPES, 1 mM MgCl2, 1 mM EGTA, pH 6.8 with KOH
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
9DXC Model of tubulin dimers used for determining the dimer rise in a taxol-stabilized microtubule-HURP complex Deposited 2024-10-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9DXE Model of tubulin dimers used for determining the dimer rise in a taxol-stabilized microtubule Deposited 2024-10-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
9EDR Tubulin Cofactors D,E,G,C and Tubulin complex -- TBCC N Terminus Bound to Tubulin Deposited 2024-11-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain B 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
9EDS Tubulin cofactors D,E,G,C bound to tubulin dimer -- TBCC N terminus unbound Deposited 2024-11-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain B 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
9EDT Tubulin cofactors D,E,G bound to tubulin dimer Deposited 2024-11-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain B 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
9EEB Tubulin cofactors D,E,G bound to tubulin dimer Deposited 2024-11-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain B 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
9GNQ Microtubule-associated Kif5B IAK tail mutant bound to ADP Deposited 2024-09-03 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 1–445（445 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8；BRB80 + 5mM AMPPNP
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9IM5 Tubulin-RB3(MUT)-TTL-Y12 Deposited 2024-07-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 4 CA CALCIUM ION × 3 CL CHLORIDE ION × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 A1L2T ~{N}4-(1,3-benzodioxol-5-ylmethyl)-6-(1~{H}-indol-4-yl)pyrimidine-2,4-diamine × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；6% PEG 4000, 5% glycerol, 0.1M MES, 30mM CaCl2, 30mM MgCl2, pH 6.7
Resolution 2.86 Å R-free 0.246
9IMO Crystal structure of Tubulin-RB3-TTL-Y12 Deposited 2024-07-04 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain B 1–431（431 aa）
Chain D 1–431（431 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 5 CA CALCIUM ION × 3 A1L2T ~{N}4-(1,3-benzodioxol-5-ylmethyl)-6-(1~{H}-indol-4-yl)pyrimidine-2,4-diamine × 4 GDP GUANOSINE-5'-DIPHOSPHATE × 2 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；6% PEG 4000, 5% glycerol, 0.1M MES, 30mM CaCl2, 30mM MgCl2, pH 6.7
Resolution 2.75 Å R-free 0.255
9KBW CryoEM structure of microtubule bound with GAS2-GAR domain. Deposited 2024-10-31 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 1–426（426 aa）
Not recorded ZN ZINC ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8；80 mM PIPES, pH 6.8, 2 mM MgCl2, 1 mM EGTA, 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
9L7M Nucleotide-free kinesin-1 motor domain bound to the microtubule Deposited 2024-12-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.48 Å
9N9E Cryo-EM structure of the SAH domain of Caenorhabditis elegans ICP-1 bound to the paclitaxel-stabilized microtubule Deposited 2025-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain B 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 ZN ZINC ION × 2 MG MAGNESIUM ION × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å
9N9F Cryo-EM structure of the SAH domain of human INCENP bound to the paclitaxel-stabilized microtubule Deposited 2025-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain B 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 ZN ZINC ION × 2 MG MAGNESIUM ION × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.91 Å
9N9G Cryo-EM structure of the human Hec1-Nuf2 dimer bound to the paclitaxel-stabilized microtubule Deposited 2025-02-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 9 PDB declaration: nonameric
Chain B 1–445（445 aa）
Chain b 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 4 GDP GUANOSINE-5'-DIPHOSPHATE × 2 TA1 TAXOL × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9N9H Cryo-EM structure of the Xenopus laevis mitotic centromere-associated kinesin (MCAK) bound to the paclitaxel-stabilized microtubule Deposited 2025-02-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain B 1–445（445 aa）
Chain b 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 4 GDP GUANOSINE-5'-DIPHOSPHATE × 2 TA1 TAXOL × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.31 Å
9N9I Cryo-EM structure of the Xenopus laevis mitotic centromere-associated kinesin (MCAK) bound to the paclitaxel-stabilized microtubule Deposited 2025-02-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 9 PDB declaration: nonameric
Chain 3 1–445（445 aa）
Chain B 1–445（445 aa）
Chain C 1–445（445 aa）
Chain b 1–445（445 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 4 TA1 TAXOL × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
9NNL Bordetella filamentous hemagglutinin (FhaB) C-terminal domain bound to microtubules Deposited 2025-03-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Chain F 1–445（445 aa）
Chain H 1–445（445 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 4 TA1 TAXOL × 4 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.42 Å
9RPD D. melanogaster Augmin TII N-clamp (GST-fusion) bound to a microtubule, well-defined subset of particles Deposited 2025-06-24 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 9 PDB declaration: nonameric
Chain B 1–445（445 aa）
Chain D 1–445（445 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.90 Å
9T1D Cryo-EM reconstruction of undecorated GDP microtubule Deposited 2025-10-21 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 1–429（429 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.90 Å
9U6A Tubulin-DARPin D1 in complex with a flavone Deposited 2025-03-22 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 1–431（431 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 A1L8X 3-methoxy-2-(4-methoxy-3-oxidanyl-phenyl)-5,7-bis(oxidanyl)chromen-4-one × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；PEG 3000, ammonium sulfate, bis-tris methane
Resolution 1.92 Å R-free 0.224
9U6D Crystal structure of tubulin-RB3-TTL in complex with X8 Deposited 2025-03-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain B 1–431（431 aa）
Chain D 1–431（431 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 5 CA CALCIUM ION × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 2 A1L8Z 2-[2-fluoranyl-4-[4-[2-[4-[(1~{R},2~{S})-2-fluoranylcyclopropyl]carbonylpiperazin-1-yl]ethoxy]phenyl]phenyl]-~{N}-(phenylmethyl)ethanamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；6% PEG 4000, 5% glycerol, 0.1M MES, 30mM CaCl2, 30mM MgCl2, pH 6.7
Resolution 2.54 Å R-free 0.238
9U9Q Tubulin-RB3-TTL in complex with C38 Deposited 2025-03-29 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain B 1–431（431 aa）
Chain D 1–431（431 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 5 CA CALCIUM ION × 7 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 A1L80 2-(1-benzofuran-5-yl)-6-[2,6-bis(fluoranyl)-4-[3-(methylamino)propoxy]phenyl]-5-chloranyl-~{N}-[(2~{S})-1,1,1-tris(fluoranyl)propan-2-yl]-[1,2,4]triazolo[1,5-a]pyrimidin-7-amine × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；6% PEG 4000, 5% glycerol, 0.1M MES, 30mM CaCl2, 30mM MgCl2, pH 6.7
Resolution 2.93 Å R-free 0.269
9YMG Human KIF18A-DARPin fusion protein bound to AMP-PNP and tubulin Deposited 2025-10-09 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 1–444（444 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 3 EDO 1,2-ETHANEDIOL × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；291 K；0.2 M ammonium nitrate, 0.1 M sodium acetate pH 5.6, 20% (w/v) PEG Smear Low (5% PEG 400, 5% PEG 500 MME, 5% PEG 600, 5% PEG 1000), 5% (v/v) ethylene glycol
Resolution 2.41 Å R-free 0.238
9YMG Human KIF18A-DARPin fusion protein bound to AMP-PNP and tubulin Deposited 2025-10-09 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain E 1–444（444 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；291 K；0.2 M ammonium nitrate, 0.1 M sodium acetate pH 5.6, 20% (w/v) PEG Smear Low (5% PEG 400, 5% PEG 500 MME, 5% PEG 600, 5% PEG 1000), 5% (v/v) ethylene glycol
Resolution 2.41 Å R-free 0.238
9YXQ Cryo-EM structure of the Xenopus laevis mitotic centromere-associated kinesin (MCAK) bound to the paclitaxel-stabilized microtubule Deposited 2025-10-27 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 14 PDB declaration: 14-meric
Chain 2 1–445（445 aa）
Chain 4 1–445（445 aa）
Chain 5 1–445（445 aa）
Chain 7 1–445（445 aa）
Chain B 1–445（445 aa）
Chain b 1–445（445 aa）
Not recorded MG MAGNESIUM ION × 11 GTP GUANOSINE-5'-TRIPHOSPHATE × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 6 TA1 TAXOL × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.39 Å

137 other PDB entries and 159 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name TBB_PIG
Isoform —
PDB entities 2
Chains and sequence ranges Author chain B； PDBConstruct 1–445； UniProt 1–445

Kinesin-like protein KIF7

Homo sapiens

UniProt Q2M1P5

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 3 PDB declaration: trimeric（3） Consistent with protein copy count Chain C； UniProt 1–398 Not recorded Tubulin alpha-1A chain × 1 （P02550） Tubulin beta chain × 1 （P02554） GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 6.8 cryo-EM vitrification conditions：Cryogen ETHANE；Blotted from behind the grid for 2 seconds Resolution 4.20 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 6MLR Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
2XT3 HUMAN KIF7, A KINESIN INVOLVED IN HEDGEHOG SIGNALLING Deposited 2010-10-04 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 7–347（341 aa） Fragment：RESIDUES 7-347
Mutation：YES ADP ADENOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6；pH 6.0
Resolution 1.88 Å R-free 0.229
4A14 HUMAN KIF7, A KINESIN INVOLVED IN HEDGEHOG SIGNALLING Deposited 2011-09-14 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 8–347（340 aa） Fragment：MOTOR DOMAIN, RESIDUES 8-347
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 20% POLYETHYLENE GLYCOL 3350, 0.20 M LITHIUM ACETATE.
Resolution 1.60 Å R-free 0.207
6MLQ Cryo-EM structure of microtubule-bound Kif7 in the ADP state Deposited 2018-09-27 Different ligand/ion Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain C 1–398（398 aa）
Not recorded MG MAGNESIUM ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE；Blotted from behind the grid for 2 seconds
Resolution 4.20 Å
7RX0 Complex of AMPPNP-Kif7 and Gli2 Zinc-Finger domain bound to microtubules Deposited 2021-08-20 Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain C 1–543（543 aa）
Not recorded ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8
cryo-EM vitrification conditions Cryogen ETHANE；Blotted from behind the grid for 2 seconds
Resolution 3.89 Å

4 other PDB entries and 4 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name KIF7_HUMAN
Isoform —
PDB entities 3
Chains and sequence ranges Author chain C； PDBConstruct 2–399； UniProt 1–398

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 6mlr

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 6mlr
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2. Structure Basics 2. Structure Basics

Entry ID entry_id6mlr
Deposition date deposition_date2018-09-27
Structure title titleCryo-EM structure of microtubule-bound Kif7 in the AMPPNP state
Keywords keywordsMicrotubule tip-tracking, Primary cilium, Hedgehog signaling, MOTOR PROTEIN, motor protein-inhibitor complex; motor protein/inhibitor
Experimental Method methodELECTRON MICROSCOPY
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier33.33 Å
Radius of gyration Rg (electron density) rg_electron32.78 Å
Forward intensity I(0) i0278334000.00
Molecular weight molecular_weight130690.0 kDa
Excluded volume excluded_volume161990 ų
Envelope volume envelope_volume207030 ų
Hydration-shell volume shell_volume51162 ų
Envelope diameter envelope_diameter119.9 Å
Shell Rg shell_rg40.89 Å
Envelope Rg envelope_rg33.02 Å
Shape Rg shape_rg32.78 Å
Total Rg total_rg33.35 Å
Total atoms total_atoms9177
Residues n_residues1160
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax110.1 Å
Rg (real space) rg_real33.26 Å
Rg uncertainty (real space) rg_real_error0.73 Å
I(0) (real space) i0_real2.7830e+08
I(0) uncertainty (real space) i0_real_error4.3950e+06
Rg (reciprocal space) rg_reciprocal33.31 Å
I(0) (reciprocal space) i0_reciprocal278300000.0000
Solution quality estimate total_estimate0.8886
Solution quality rating solution_quality GOOD a GOOD solution
P(r) peaks n_peaks2
Primary peak position r_peak_primary39.0 Å
Skewness Skewness skewness0.284
Kurtosis Kurtosis kurtosis-0.398
Angular range angular_range— – 0.2400 Å−1
Current regularization parameter α current_alpha0.0000
Highest regularization parameter α highest_alpha103100000.0000
Real-space data points n_real_points49
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.865; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.999; Smooth: 0.952
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (7)

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8. Citations (1)

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9. Files and Curves (10)