Current Protein Identity:P13393 New Search
Main Difference Dimensions in This Set
Different construct Different mutation/modification Different assembly state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics

Difference tags compare only the current result set; every original PDB and assembly record remains separate.

Related-Structure Differences

Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.

PDB Entry Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Experimental Method Experimental Conditions Structure Quality
1NGM Crystal structure of a yeast Brf1-TBP-DNA ternary complex Deposited 2002-12-17 Assembly 1 Protein–DNA Heteromer;Protein × 2 PDB declaration: tetrameric(4) Consistent with all polymers
Chain A 60–239(180 aa) Fragment:C-terminal core domain
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP;pH 6.5;277 K;MES, NaCl, BME, glycerol, MgCl2, PEG2000MME, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.95 Å R-free 0.308
1NGM Crystal structure of a yeast Brf1-TBP-DNA ternary complex Deposited 2002-12-17 Assembly 2 Protein–DNA Heteromer;Protein × 2 PDB declaration: tetrameric(4) Consistent with all polymers
Chain E 60–239(180 aa) Fragment:C-terminal core domain
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP;pH 6.5;277 K;MES, NaCl, BME, glycerol, MgCl2, PEG2000MME, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.95 Å R-free 0.308
1NGM Crystal structure of a yeast Brf1-TBP-DNA ternary complex Deposited 2002-12-17 Assembly 3 Protein–DNA Heteromer;Protein × 2 PDB declaration: tetrameric(4) Consistent with all polymers
Chain I 60–239(180 aa) Fragment:C-terminal core domain
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP;pH 6.5;277 K;MES, NaCl, BME, glycerol, MgCl2, PEG2000MME, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.95 Å R-free 0.308
1NGM Crystal structure of a yeast Brf1-TBP-DNA ternary complex Deposited 2002-12-17 Assembly 4 Protein–DNA Heteromer;Protein × 2 PDB declaration: tetrameric(4) Consistent with all polymers
Chain M 60–239(180 aa) Fragment:C-terminal core domain
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP;pH 6.5;277 K;MES, NaCl, BME, glycerol, MgCl2, PEG2000MME, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.95 Å R-free 0.308
1NH2 Crystal structure of a yeast TFIIA/TBP/DNA complex Deposited 2002-12-18 Assembly 1 Protein–DNA Heteromer;Protein × 4 PDB declaration: hexameric(6) Consistent with all polymers
Chain A 60–239(180 aa) Fragment:C-TERMINAL 180 RESIDUES
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.90 Å R-free 0.226
1RM1 Structure of a Yeast TFIIA/TBP/TATA-box DNA Complex Deposited 2003-11-26 Assembly 1 Protein–DNA Heteromer;Protein × 3 PDB declaration: pentameric(5) Consistent with all polymers
Chain A 1–239(239 aa)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP;pH 5.6;291 K;PEG 4000, ammonium citrate, MES, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 2.50 Å R-free 0.252
1TBA SOLUTION STRUCTURE OF A TBP-TAFII230 COMPLEX: PROTEIN MIMICRY OF THE MINOR GROOVE SURFACE OF THE TATA BOX UNWOUND BY TBP, NMR, 25 STRUCTURES Deposited 1998-08-16 Assembly 1 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count
Chain B 60–239(180 aa) Fragment:CORE DOMAIN RESIDUES 60-249
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 7;298 K
Resolution not provided
1TBP CRYSTAL STRUCTURE OF YEAST TATA-BINDING PROTEIN AND MODEL FOR INTERACTION WITH DNA Deposited 1993-08-02 Assembly 1 Protein homooligomer Homooligomer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count
Chain A 61–239(179 aa)
Chain B 61–239(179 aa)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.60 Å
1YTB CRYSTAL STRUCTURE OF A YEAST TBP/TATA-BOX COMPLEX Deposited 1994-09-28 Assembly 1 Protein–DNA Monomer;Protein × 1 PDB declaration: dimeric(2) Consistent with all polymers
Chain A 60–239(180 aa)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP;pH 7.5;295 K;pH 7.50, VAPOR DIFFUSION, HANGING DROP, temperature 295.00K
Resolution 1.80 Å R-free 0.261
1YTB CRYSTAL STRUCTURE OF A YEAST TBP/TATA-BOX COMPLEX Deposited 1994-09-28 Assembly 2 Protein–DNA Monomer;Protein × 1 PDB declaration: dimeric(2) Consistent with all polymers
Chain B 60–239(180 aa)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP;pH 7.5;295 K;pH 7.50, VAPOR DIFFUSION, HANGING DROP, temperature 295.00K
Resolution 1.80 Å R-free 0.261
1YTF YEAST TFIIA/TBP/DNA COMPLEX Deposited 1996-04-05 Assembly 1 Protein–DNA Heteromer;Protein × 4 PDB declaration: hexameric(6) Consistent with all polymers
Chain A 60–239(180 aa)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.50 Å R-free 0.297
4B0A The high-resolution structure of yTBP-yTAF1 identifies conserved and competing interaction surfaces in transcriptional activation Deposited 2012-06-29 Assembly 1 Insufficient information Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count
Chain A 61–240(180 aa) Fragment:TAF1 RESIDUES 8-71, LINKER, TBP RESIDUE 61-240
Mutation:YES CA CALCIUM ION × 3 GOL GLYCEROL × 6 CL CHLORIDE ION × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.97 Å R-free 0.223
4V1N Architecture of the RNA polymerase II-Mediator core transcription initiation complex Deposited 2014-09-29 Assembly 1 Other combination Heteromer;Protein × 16 PDB declaration: nonadecameric(19) Consistent with all polymers
Chain O 61–240(180 aa) Fragment:RESIDUES 61-240
Not recorded ZN ZINC ION × 9 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer 25 MM HEPES-KOH PH 7.5, 180 MM POTASSIUM ACETATE, 5 % GLYCEROL, 5 MM DTT;pH 7.5;25 MM HEPES-KOH PH 7.5, 180 MM POTASSIUM ACETATE, 5 % GLYCEROL, 5 MM DTT
cryo-EM vitrification conditions Cryogen ETHANE;GRIDS WERE GLOW- DISCHARGED FOR 20 S BEFORE DEPOSITION OF 4 MICROLITERS SAMPLE AND INCUBATED FOR 30 S. GRIDS WERE WASHED TWICE WITH 4 MICROLITERS DISTILLED WATER, BLOTTED, AND VITRIFIED BY PLUNGING INTO LIQUID ETHANE WITH A MANUAL PLUNGER.
Resolution 7.80 Å
4V1O Architecture of the RNA polymerase II-Mediator core transcription initiation complex Deposited 2014-09-29 Assembly 1 Other combination Heteromer;Protein × 23 PDB declaration: 26-meric(26) Consistent with all polymers
Chain O 61–240(180 aa) Fragment:RESIDUES 61-240
Not recorded ZN ZINC ION × 9 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer 25 MM HEPES-KOH PH 7.5, 180 MM POTASSIUM ACETATE, 5 % GLYCEROL, 5 MM DTT;pH 7.5;25 MM HEPES-KOH PH 7.5, 180 MM POTASSIUM ACETATE, 5 % GLYCEROL, 5 MM DTT
cryo-EM vitrification conditions Cryogen ETHANE;GRIDS WERE GLOW- DISCHARGED FOR 20 S BEFORE DEPOSITION OF 4 MICROLITERS SAMPLE AND INCUBATED FOR 30 S. GRIDS WERE WASHED TWICE WITH 4 MICROLITERS DISTILLED WATER, BLOTTED, AND VITRIFIED BY PLUNGING INTO LIQUID ETHANE WITH A MANUAL PLUNGER.
Resolution 9.70 Å
5FMF the P-lobe of RNA polymerase II pre-initiation complex Deposited 2015-11-03 Assembly 1 Protein–DNA Heteromer;Protein × 25 PDB declaration: 27-meric(27) Consistent with all polymers
Chain Q 61–240(180 aa)
Not recorded MG MAGNESIUM ION × 2 ZN ZINC ION × 9 ELECTRON MICROSCOPY
cryo-EM buffer 20 MM HEPES (PH7.6), 5 MM DTT, 2 MM MG(OAC)2,AND 40 MM KOAC;pH 7.6;20 MM HEPES (PH7.6), 5 MM DTT, 2 MM MG(OAC)2,AND 40 MM KOAC
cryo-EM vitrification conditions Cryogen ETHANE;VITRIFICATION 1 -- CRYOGEN- ETHANE, HUMIDITY- 100, TEMPERATURE- 120, INSTRUMENT- FEI VITROBOT MARK III,
Resolution 6.00 Å
5FYW Transcription initiation complex structures elucidate DNA opening (OC) Deposited 2016-03-10 Assembly 1 Protein–DNA Heteromer;Protein × 20 PDB declaration: 22-meric(22) Consistent with all polymers
Chain O 1–240(240 aa)
Not recorded ZN ZINC ION × 10 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer 25 MM HEPES-KOH PH 7.5, 150 MM POTASSIUM ACETATE, 2 MM MGCL2, 5 MM DTT;pH 7.5;25 MM HEPES-KOH PH 7.5, 150 MM POTASSIUM ACETATE, 2 MM MGCL2, 5 MM DTT
cryo-EM vitrification conditions Cryogen ETHANE;CRYOGEN - ETHANE. INSTRUMENT - FEI VITROBOT MARK IV. QUANTIFOIL R 3.5- 1 HOLEY CARBON GRIDS WERE GLOW-DISCHARGED BEFORE DEPOSITION OF 4.5 MICROLITERS OF SAMPLE. GRIDS WERE THEN BLOTTED FOR 8.5 S AND PLUNGE- FROZEN IN LIQUID ETHANE.
Resolution 4.35 Å
5FZ5 Transcription initiation complex structures elucidate DNA opening (CC) Deposited 2016-03-10 Assembly 1 Protein–DNA Heteromer;Protein × 20 PDB declaration: 22-meric(22) Consistent with all polymers
Chain O 1–240(240 aa)
Not recorded ZN ZINC ION × 10 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer 25 MM HEPES-KOH PH 7.5, 150 MM POTASSIUM ACETATE, 2 MM MGCL2, 5 MM DTT;pH 7.5;25 MM HEPES-KOH PH 7.5, 150 MM POTASSIUM ACETATE, 2 MM MGCL2, 5 MM DTT
cryo-EM vitrification conditions Cryogen ETHANE;LIQUID ETHANE
Resolution 8.80 Å
5OQJ STRUCTURE OF YEAST TRANSCRIPTION PRE-INITIATION COMPLEX WITH TFIIH Deposited 2017-08-11 Assembly 1 Protein–DNA Heteromer;Protein × 29 PDB declaration: 31-meric(31) Consistent with all polymers
Chain O 1–240(240 aa)
Not recorded ZN ZINC ION × 16 MG MAGNESIUM ION × 1 SF4 IRON/SULFUR CLUSTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE;GRIDS WERE BLOTTED FOR 8 S AND PLUNGE-FROZEN IN LIQUID ETHANE.
Resolution 4.70 Å
5OQM STRUCTURE OF YEAST TRANSCRIPTION PRE-INITIATION COMPLEX WITH TFIIH AND CORE MEDIATOR Deposited 2017-08-13 Assembly 1 Insufficient information Heteromer;Protein × 44 PDB declaration: 46-meric(46) Consistent with all polymers
Chain O 1–240(240 aa)
Not recorded ZN ZINC ION × 16 MG MAGNESIUM ION × 1 SF4 IRON/SULFUR CLUSTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE;Grids were blotted for 8 s and plunge-frozen in liquid ethane.
Resolution 5.80 Å
5SVA Mediator-RNA Polymerase II Pre-Initiation Complex Deposited 2016-08-05 Assembly 1 Protein–DNA Heteromer;Protein × 38 PDB declaration: 40-meric(40) Consistent with all polymers
Chain j 1–240(240 aa)
Not recorded ZN ZINC ION × 8 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 15.30 Å
6CNB Yeast RNA polymerase III initial transcribing complex Deposited 2018-03-08 Assembly 1 Insufficient information Heteromer;Protein × 19 PDB declaration: 21-meric(21) Consistent with all polymers
Chain R 61–240(180 aa)
Not recorded ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
6CNC Yeast RNA polymerase III open complex Deposited 2018-03-08 Assembly 1 Insufficient information Heteromer;Protein × 19 PDB declaration: 21-meric(21) Consistent with all polymers
Chain R 61–240(180 aa)
Mutation:C438S ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
6CND Yeast RNA polymerase III natural open complex (nOC) Deposited 2018-03-08 Assembly 1 Insufficient information Heteromer;Protein × 19 PDB declaration: 21-meric(21) Consistent with all polymers
Chain R 61–240(180 aa)
Mutation:C438S ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.80 Å
6CNF Yeast RNA polymerase III elongation complex Deposited 2018-03-08 Assembly 1 Insufficient information Heteromer;Protein × 19 PDB declaration: 21-meric(21) Consistent with all polymers
Chain R 61–240(180 aa)
Mutation:C438S ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.50 Å
6E16 Ternary structure of c-Myc-TBP-TAF1 Deposited 2018-07-09 Assembly 1 Insufficient information Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count
Chain A 61–240(180 aa)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions BATCH MODE;291 K;20% (w/w) PEG8000, 0.2M ammonium sulfare, and 0.1M HEPES pH7.5
Resolution 2.40 Å R-free 0.220
6E24 Ternary structure of c-Myc-TBP-TAF1 Deposited 2018-07-10 Assembly 1 Insufficient information Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count
Chain A 61–240(180 aa)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions EVAPORATION;293 K;16% W/V PEG3350 0.05M CITRIC ACID 0.05M BIS-TRIS PROPANE pH5.0
Resolution 3.00 Å R-free 0.224
6EU0 RNA Polymerase III open pre-initiation complex (OC-PIC) Deposited 2017-10-27 Assembly 1 Protein–DNA Heteromer;Protein × 20 PDB declaration: 22-meric(22) Consistent with all polymers
Chain Y 1–240(240 aa)
Not recorded ZN ZINC ION × 7 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.00 Å
6F40 RNA Polymerase III open complex Deposited 2017-11-29 Assembly 1 Protein–DNA Heteromer;Protein × 20 PDB declaration: 22-meric(22) Consistent with all polymers
Chain U 1–240(240 aa)
Not recorded ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
6F41 RNA Polymerase III initially transcribing complex Deposited 2017-11-29 Assembly 1 Other combination Heteromer;Protein × 20 PDB declaration: 23-meric(23) Consistent with all polymers
Chain U 1–240(240 aa)
Not recorded ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
6F42 RNA Polymerase III closed complex CC1. Deposited 2017-11-29 Assembly 1 Protein–DNA Heteromer;Protein × 20 PDB declaration: 22-meric(22) Consistent with all polymers
Chain U 1–240(240 aa)
Not recorded ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.50 Å
6F44 RNA Polymerase III closed complex CC2. Deposited 2017-11-29 Assembly 1 Protein–DNA Heteromer;Protein × 20 PDB declaration: 22-meric(22) Consistent with all polymers
Chain U 1–240(240 aa)
Not recorded ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
6GYK Structure of a yeast closed complex (core CC1) Deposited 2018-06-30 Assembly 1 Protein–DNA Heteromer;Protein × 18 PDB declaration: eicosameric(20) Consistent with all polymers
Chain O 1–240(240 aa)
Not recorded ZN ZINC ION × 9 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.10 Å
6GYL Structure of a yeast closed complex with distorted DNA (core CCdist) Deposited 2018-06-30 Assembly 1 Protein–DNA Heteromer;Protein × 20 PDB declaration: 22-meric(22) Consistent with all polymers
Chain O 1–240(240 aa)
Not recorded ZN ZINC ION × 10 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.80 Å
6GYM Structure of a yeast closed complex with distorted DNA (CCdist) Deposited 2018-06-30 Assembly 1 Protein–DNA Heteromer;Protein × 29 PDB declaration: 31-meric(31) Consistent with all polymers
Chain O 1–240(240 aa)
Not recorded SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 16 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.70 Å
7ML0 RNA polymerase II pre-initiation complex (PIC1) Deposited 2021-04-27 Assembly 1 Protein–DNA Heteromer;Protein × 26 PDB declaration: 28-meric(28) Consistent with all polymers
Chain O 1–240(240 aa)
Not recorded ZN ZINC ION × 17 MG MAGNESIUM ION × 1 SF4 IRON/SULFUR CLUSTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
7ML1 RNA polymerase II pre-initiation complex (PIC2) Deposited 2021-04-27 Assembly 1 Protein–DNA Heteromer;Protein × 28 PDB declaration: 30-meric(30) Consistent with all polymers
Chain O 1–240(240 aa)
Not recorded ZN ZINC ION × 19 SF4 IRON/SULFUR CLUSTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.00 Å
7ML4 RNA polymerase II initially transcribing complex (ITC) Deposited 2021-04-27 Assembly 1 Other combination Heteromer;Protein × 28 PDB declaration: 31-meric(31) Consistent with all polymers
Chain O 1–240(240 aa)
Not recorded ZN ZINC ION × 15 MG MAGNESIUM ION × 1 SF4 IRON/SULFUR CLUSTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7O4I Yeast RNA polymerase II transcription pre-initiation complex with initial transcription bubble Deposited 2021-04-06 Assembly 1 Protein–DNA Heteromer;Protein × 28 PDB declaration: 30-meric(30) Consistent with all polymers
Chain O 1–240(240 aa)
Not recorded SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 17 BEF BERYLLIUM TRIFLUORIDE ION × 1 MG MAGNESIUM ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
7O4J Yeast RNA polymerase II transcription pre-initiation complex (consensus) Deposited 2021-04-06 Assembly 1 Protein–DNA Heteromer;Protein × 28 PDB declaration: 30-meric(30) Consistent with all polymers
Chain O 1–240(240 aa)
Not recorded SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 17 BEF BERYLLIUM TRIFLUORIDE ION × 1 MG MAGNESIUM ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
7O72 Yeast RNA polymerase II transcription pre-initiation complex with closed promoter DNA Deposited 2021-04-12 Assembly 1 Protein–DNA Heteromer;Protein × 28 PDB declaration: 30-meric(30) Consistent with all polymers
Chain O 1–240(240 aa)
Not recorded SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 17 BEF BERYLLIUM TRIFLUORIDE ION × 1 MG MAGNESIUM ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
7O73 Yeast RNA polymerase II transcription pre-initiation complex with closed distorted promoter DNA Deposited 2021-04-12 Assembly 1 Protein–DNA Heteromer;Protein × 28 PDB declaration: 30-meric(30) Consistent with all polymers
Chain O 1–240(240 aa)
Not recorded SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 17 BEF BERYLLIUM TRIFLUORIDE ION × 1 MG MAGNESIUM ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
7O75 Yeast RNA polymerase II transcription pre-initiation complex with open promoter DNA Deposited 2021-04-13 Assembly 1 Protein–DNA Heteromer;Protein × 28 PDB declaration: 30-meric(30) Consistent with all polymers
Chain O 1–240(240 aa)
Not recorded SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 17 BEF BERYLLIUM TRIFLUORIDE ION × 1 MG MAGNESIUM ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
7Q5B Cryo-EM structure of Ty3 retrotransposon targeting a TFIIIB-bound tRNA gene Deposited 2021-11-03 Assembly 1 Protein–DNA Heteromer;Protein × 7 PDB declaration: tridecameric(13) Consistent with all polymers
Chain Y 1–240(240 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.98 Å
7Z0O Structure of transcription factor UAF in complex with TBP and 35S rRNA promoter DNA Deposited 2022-02-23 Assembly 1 Protein–DNA Heteromer;Protein × 8 PDB declaration: decameric(10) Consistent with all polymers
Chain H 1–240(240 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.2
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
7ZS9 Yeast RNA polymerase II transcription pre-initiation complex with the +1 nucleosome (complex A) Deposited 2022-05-06 Assembly 1 Protein–DNA Heteromer;Protein × 36 PDB declaration: 38-meric(38) Consistent with all polymers
Chain O 1–240(240 aa)
Not recorded ZN ZINC ION × 17 MG MAGNESIUM ION × 1 SF4 IRON/SULFUR CLUSTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7ZSA Yeast RNA polymerase II transcription pre-initiation complex with the +1 nucleosome and NTP (complex B) Deposited 2022-05-06 Assembly 1 Protein–DNA Heteromer;Protein × 36 PDB declaration: 38-meric(38) Consistent with all polymers
Chain O 1–240(240 aa)
Not recorded ZN ZINC ION × 17 MG MAGNESIUM ION × 1 SF4 IRON/SULFUR CLUSTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.00 Å
7ZSB Yeast RNA polymerase II transcription pre-initiation complex with the +1 nucleosome and NTP, complex C Deposited 2022-05-06 Assembly 1 Protein–DNA Heteromer;Protein × 36 PDB declaration: 38-meric(38) Consistent with all polymers
Chain O 1–240(240 aa)
Not recorded ZN ZINC ION × 17 MG MAGNESIUM ION × 1 SF4 IRON/SULFUR CLUSTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.60 Å
8FFZ TFIIIA-TFIIIC-Brf1-TBP complex bound to 5S rRNA gene Deposited 2022-12-11 Assembly 1 Insufficient information Heteromer;Protein × 8 PDB declaration: decameric(10) Consistent with all polymers
Chain H 61–240(180 aa)
Not recorded ZN ZINC ION × 9 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
8H5B The cryo-EM structure of nuclear transport receptor Kap114p complex with yeast TATA-box binding protein Deposited 2022-10-12 Assembly 1 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count
Chain B 61–240(180 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.03 Å
8UMH Consensus map of PICdeltaTFIIK form2 Deposited 2023-10-17 Assembly 1 Protein–DNA Heteromer;Protein × 28 PDB declaration: 30-meric(30) Consistent with all polymers
Chain O 1–240(240 aa)
Not recorded ZN ZINC ION × 15 MG MAGNESIUM ION × 2 SF4 IRON/SULFUR CLUSTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6;20 mM HEPES PH 7.6 50 mM KOAc 5 mM DTT 2 mM MgOAc
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
8UMI consensus map of PICdeltaTFIIK form1 Deposited 2023-10-17 Assembly 1 Protein–DNA Heteromer;Protein × 28 PDB declaration: 30-meric(30) Consistent with all polymers
Chain O 1–240(240 aa)
Not recorded SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 15 MG MAGNESIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6;20 mM HEPES PH 7.6 50 mM KOAc 5 mM DTT 2 mM MgOAc
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
8UOQ Composite map of PIC_delta_TFIIK form2 Deposited 2023-10-20 Assembly 1 Protein–DNA Heteromer;Protein × 28 PDB declaration: 30-meric(30) Consistent with all polymers
Chain O 1–240(240 aa)
Not recorded ZN ZINC ION × 15 MG MAGNESIUM ION × 2 SF4 IRON/SULFUR CLUSTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
8UOT Composite map of PICdeltaTFIIK form1 Deposited 2023-10-20 Assembly 1 Protein–DNA Heteromer;Protein × 28 PDB declaration: 30-meric(30) Consistent with all polymers
Chain O 1–240(240 aa)
Not recorded SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 15 MG MAGNESIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6;20 mM HEPES PH 7.6 50 mM KOAc 5 mM DTT 2 mM MgOAc
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å