Current Protein Identity:P28000
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 4C2M Structure of RNA polymerase I at 2.8 A resolution Deposited 2013-08-19 | Assembly 1 Protein heterocomplex Heteromer;Protein × 15 PDB declaration: pentadecameric(15) Consistent with protein count |
Chain K
1–142(142 aa)
|
Not recorded | ZN ZINC ION × 7 SO4 SULFATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7;10% PEG4000, 300MM AMMONIUM ACETATE, 50MM HEPES PH 7.5, 5MM TCEP
|
Resolution 2.80 Å R-free 0.210 |
| 4C2M Structure of RNA polymerase I at 2.8 A resolution Deposited 2013-08-19 | Assembly 2 Protein heterocomplex Heteromer;Protein × 15 PDB declaration: pentadecameric(15) Consistent with protein count |
Chain Z
1–142(142 aa)
|
Not recorded | ZN ZINC ION × 7 SO4 SULFATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7;10% PEG4000, 300MM AMMONIUM ACETATE, 50MM HEPES PH 7.5, 5MM TCEP
|
Resolution 2.80 Å R-free 0.210 |
| 4C3H Structure of 14-subunit RNA polymerase I at 3.27 A resolution, crystal form C2-93 Deposited 2013-08-24 | Assembly 1 Protein heterocomplex Heteromer;Protein × 14 PDB declaration: tetradecameric(14) Consistent with protein count |
Chain K
1–142(142 aa)
|
Not recorded | ZN ZINC ION × 7 | X-RAY DIFFRACTION |
X-ray crystallization conditions
24% ETHYLENGLYCOL, 0.1M MES PH 6.8
|
Resolution 3.27 Å R-free 0.226 |
| 4C3I Structure of 14-subunit RNA polymerase I at 3.0 A resolution, crystal form C2-100 Deposited 2013-08-24 | Assembly 1 Protein heterocomplex Heteromer;Protein × 14 PDB declaration: tetradecameric(14) Consistent with protein count |
Chain K
1–142(142 aa)
|
Not recorded | SO4 SULFATE ION × 3 ZN ZINC ION × 7 MG MAGNESIUM ION × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
10% MPD, 0.1M TRIS HCL PH 7.4
|
Resolution 3.00 Å R-free 0.231 |
| 4C3J Structure of 14-subunit RNA polymerase I at 3.35 A resolution, crystal form C2-90 Deposited 2013-08-24 | Assembly 1 Protein heterocomplex Heteromer;Protein × 14 PDB declaration: tetradecameric(14) Consistent with protein count |
Chain K
1–142(142 aa)
|
Not recorded | ZN ZINC ION × 7 | X-RAY DIFFRACTION |
X-ray crystallization conditions
10% MPD, 0.1M TRIS HCL, PH 7.5
|
Resolution 3.35 Å R-free 0.220 |
| 4YM7 RNA polymerase I structure with an alternative dimer hinge Deposited 2015-03-06 | Assembly 1 Protein heterocomplex Heteromer;Protein × 15 PDB declaration: pentadecameric(15) Consistent with protein count |
Chain AK
1–142(142 aa)
|
Not recorded | ZN ZINC ION × 7 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP;pH 7.5;293 K;10% PEG 6000, 3% MPD, 100 mM Hepes, 300 mM di-ammonium-tartrate, 3 mM TCEP
|
Resolution 5.50 Å R-free 0.235 |
| 4YM7 RNA polymerase I structure with an alternative dimer hinge Deposited 2015-03-06 | Assembly 2 Protein heterocomplex Heteromer;Protein × 15 PDB declaration: pentadecameric(15) Consistent with protein count |
Chain BK
1–142(142 aa)
|
Not recorded | ZN ZINC ION × 7 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP;pH 7.5;293 K;10% PEG 6000, 3% MPD, 100 mM Hepes, 300 mM di-ammonium-tartrate, 3 mM TCEP
|
Resolution 5.50 Å R-free 0.235 |
| 4YM7 RNA polymerase I structure with an alternative dimer hinge Deposited 2015-03-06 | Assembly 3 Protein heterocomplex Heteromer;Protein × 15 PDB declaration: pentadecameric(15) Consistent with protein count |
Chain CK
1–142(142 aa)
|
Not recorded | ZN ZINC ION × 7 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP;pH 7.5;293 K;10% PEG 6000, 3% MPD, 100 mM Hepes, 300 mM di-ammonium-tartrate, 3 mM TCEP
|
Resolution 5.50 Å R-free 0.235 |
| 4YM7 RNA polymerase I structure with an alternative dimer hinge Deposited 2015-03-06 | Assembly 4 Protein heterocomplex Heteromer;Protein × 15 PDB declaration: pentadecameric(15) Consistent with protein count |
Chain DK
1–142(142 aa)
|
Not recorded | ZN ZINC ION × 7 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP;pH 7.5;293 K;10% PEG 6000, 3% MPD, 100 mM Hepes, 300 mM di-ammonium-tartrate, 3 mM TCEP
|
Resolution 5.50 Å R-free 0.235 |
| 4YM7 RNA polymerase I structure with an alternative dimer hinge Deposited 2015-03-06 | Assembly 5 Protein heterocomplex Heteromer;Protein × 15 PDB declaration: pentadecameric(15) Consistent with protein count |
Chain EK
1–142(142 aa)
|
Not recorded | ZN ZINC ION × 7 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP;pH 7.5;293 K;10% PEG 6000, 3% MPD, 100 mM Hepes, 300 mM di-ammonium-tartrate, 3 mM TCEP
|
Resolution 5.50 Å R-free 0.235 |
| 4YM7 RNA polymerase I structure with an alternative dimer hinge Deposited 2015-03-06 | Assembly 6 Protein heterocomplex Heteromer;Protein × 15 PDB declaration: pentadecameric(15) Consistent with protein count |
Chain FK
1–142(142 aa)
|
Not recorded | ZN ZINC ION × 7 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP;pH 7.5;293 K;10% PEG 6000, 3% MPD, 100 mM Hepes, 300 mM di-ammonium-tartrate, 3 mM TCEP
|
Resolution 5.50 Å R-free 0.235 |
| 5FJ8 Cryo-EM structure of yeast RNA polymerase III elongation complex at 3. 9 A Deposited 2015-10-06 | Assembly 1 Other combination Heteromer;Protein × 17 PDB declaration: eicosameric(20) Consistent with all polymers |
Chain K
1–142(142 aa)
|
Not recorded | ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
150MM (NH4)2SO4, 15MM TRIS, 10MM DTT, 5MM MGCL2;pH 7.5;150MM (NH4)2SO4, 15MM TRIS, 10MM DTT, 5MM MGCL2
cryo-EM vitrification conditions
Cryogen ETHANE;VITRIFICATION 1 -- CRYOGEN- ETHANE, HUMIDITY- 95, INSTRUMENT- FEI VITROBOT MARK III, METHOD- 2.5 UL OF SAMPLE WAS APPLIED 15 SECONDS WAIT TIME BLOT FOR 9 SECONDS BEFORE PLUNGING,
|
Resolution 3.90 Å |
| 5FJ9 Cryo-EM structure of yeast apo RNA polymerase III at 4.6 A Deposited 2015-10-06 | Assembly 1 Protein heterocomplex Heteromer;Protein × 17 PDB declaration: heptadecameric(17) Consistent with protein count |
Chain K
1–142(142 aa)
|
Not recorded | ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
150MM (NH4)2SO4, 15MM TRIS, 10MM DTT;pH 7.5;150MM (NH4)2SO4, 15MM TRIS, 10MM DTT
cryo-EM vitrification conditions
Cryogen ETHANE;VITRIFICATION 1 -- CRYOGEN- ETHANE, HUMIDITY- 95, INSTRUMENT- FEI VITROBOT MARK III, METHOD- 2.5 UL OF SAMPLE WAS APPLIED 15 SECONDS WAIT TIME BLOT FOR 9 SECONDS BEFORE PLUNGING,
|
Resolution 4.60 Å |
| 5FJA Cryo-EM structure of yeast RNA polymerase III at 4.7 A Deposited 2015-10-06 | Assembly 1 Protein heterocomplex Heteromer;Protein × 17 PDB declaration: heptadecameric(17) Consistent with protein count |
Chain K
1–142(142 aa)
|
Not recorded | ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
150MM (NH4)2SO4, 15MM TRIS, 10MM DTT;pH 7.5;150MM (NH4)2SO4, 15MM TRIS, 10MM DTT
cryo-EM vitrification conditions
Cryogen ETHANE;VITRIFICATION 1 -- CRYOGEN- ETHANE, HUMIDITY- 95, INSTRUMENT - FEI VITROBOT MARK III, METHOD- 2.5 UL OF SAMPLE WAS APPLIED 15 SECONDS WAIT TIME BLOT FOR 9 SECONDS BEFORE PLUNGING,
|
Resolution 4.65 Å |
| 5G5L RNA polymerase I-Rrn3 complex at 4.8 A resolution Deposited 2016-05-26 | Assembly 1 Protein heterocomplex Heteromer;Protein × 15 PDB declaration: pentadecameric(15) Consistent with protein count |
Chain K
1–142(142 aa)
|
Not recorded | ZN ZINC ION × 7 | ELECTRON MICROSCOPY |
cryo-EM buffer
150MM NACL, 5MM HEPES, 1MM MGCL2, 0.01 MM ZNCL2, 5 MM DTT;pH 7.8;150MM NACL, 5MM HEPES, 1MM MGCL2, 0.01 MM ZNCL2, 5 MM DTT
cryo-EM vitrification conditions
Cryogen ETHANE;VITROBOT MARK IV, METHOD- 4.5 MICROLITERS OF SAMPLE WAS APPLIED TO GLOW- DISCHARGED QUANTIFOIL R 2- 1 HOLEY CARBON GRIDS, WHICH WERE THEN BLOTTED FOR 8.5S AND PLUNGE- FROZEN IN LIQUID ETHANE.
|
Resolution 4.80 Å |
| 5LMX Monomeric RNA polymerase I at 4.9 A resolution Deposited 2016-08-02 | Assembly 1 Protein heterocomplex Heteromer;Protein × 14 PDB declaration: tetradecameric(14) Consistent with protein count |
Chain K
1–142(142 aa)
|
Not recorded | ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.90 Å |
| 5M3F Yeast RNA polymerase I elongation complex at 3.8A Deposited 2016-10-14 | Assembly 1 Other combination Heteromer;Protein × 14 PDB declaration: heptadecameric(17) Consistent with all polymers |
Chain K
1–142(142 aa)
|
Not recorded | ZN ZINC ION × 6 MG MAGNESIUM ION × 1 SO4 SULFATE ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.8
cryo-EM vitrification conditions
Cryogen ETHANE;blot force 13, blotting time 8.5s
|
Resolution 3.80 Å |
| 5M3M Free monomeric RNA polymerase I at 4.0A resolution Deposited 2016-10-15 | Assembly 1 Protein heterocomplex Heteromer;Protein × 14 PDB declaration: tetradecameric(14) Consistent with protein count |
Chain K
1–142(142 aa)
|
Not recorded | ZN ZINC ION × 7 SO4 SULFATE ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.8
cryo-EM vitrification conditions
Cryogen ETHANE;blot force 13, blotting time 8.5s
|
Resolution 4.00 Å |
| 5M5W RNA Polymerase I open complex Deposited 2016-10-23 | Assembly 1 Protein–DNA Heteromer;Protein × 14 PDB declaration: hexadecameric(16) Consistent with all polymers |
Chain K
1–142(142 aa)
|
Not recorded | ZN ZINC ION × 7 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE;2.5 ul of sample
15 seconds wait time
Blot for 8 seconds
|
Resolution 3.80 Å |
| 5M5X RNA Polymerase I elongation complex 1 Deposited 2016-10-23 | Assembly 1 Other combination Heteromer;Protein × 14 PDB declaration: heptadecameric(17) Consistent with all polymers |
Chain K
1–142(142 aa)
|
Not recorded | ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE;2.5 ul of sample
15 seconds wait time
Blot time for 8 seconds
|
Resolution 4.00 Å |
| 5M5Y RNA Polymerase I elongation complex 2 Deposited 2016-10-23 | Assembly 1 Other combination Heteromer;Protein × 14 PDB declaration: heptadecameric(17) Consistent with all polymers |
Chain K
1–142(142 aa)
|
Not recorded | ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE;2.5 ul of sample
15 seconds wait time
Blot for 8 seconds
|
Resolution 4.00 Å |
| 5M64 RNA Polymerase I elongation complex with A49 tandem winged helix domain Deposited 2016-10-24 | Assembly 1 Other combination Heteromer;Protein × 14 PDB declaration: heptadecameric(17) Consistent with all polymers |
Chain K
1–142(142 aa)
|
Not recorded | ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE;2.5 ul of sample
15 seconds wait time
Blot for 8 seconds
|
Resolution 4.60 Å |
| 5N5Y Cryo-EM structure of RNA polymerase I in complex with Rrn3 and Core Factor (Orientation III) Deposited 2017-02-14 | Assembly 1 Protein heterocomplex Heteromer;Protein × 18 PDB declaration: octadecameric(18) Consistent with protein count |
Chain K
1–142(142 aa)
|
Not recorded | ZN ZINC ION × 8 SO4 SULFATE ION × 5 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 7.70 Å |
| 5N5Z Cryo-EM structure of RNA polymerase I in complex with Rrn3 and Core Factor (Orientation II) Deposited 2017-02-14 | Assembly 1 Protein heterocomplex Heteromer;Protein × 18 PDB declaration: octadecameric(18) Consistent with protein count |
Chain K
1–142(142 aa)
|
Not recorded | ZN ZINC ION × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 7.70 Å |
| 5N60 Cryo-EM structure of RNA polymerase I in complex with Rrn3 and Core Factor (Orientation I) Deposited 2017-02-14 | Assembly 1 Protein heterocomplex Heteromer;Protein × 18 PDB declaration: octadecameric(18) Consistent with protein count |
Chain K
1–142(142 aa)
|
Not recorded | ZN ZINC ION × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 7.70 Å |
| 5N61 RNA polymerase I initially transcribing complex Deposited 2017-02-14 | Assembly 1 Other combination Heteromer;Protein × 18 PDB declaration: 21-meric(21) Consistent with all polymers |
Chain K
1–142(142 aa)
|
Not recorded | ZN ZINC ION × 7 MG MAGNESIUM ION × 1 SO4 SULFATE ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 5OA1 RNA polymerase I pre-initiation complex Deposited 2017-06-20 | Assembly 1 Protein–DNA Heteromer;Protein × 32 PDB declaration: 34-meric(34) Consistent with all polymers |
Chain K
1–142(142 aa)
|
Not recorded | ZN ZINC ION × 7 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.40 Å |
| 5W5Y RNA polymerase I Initial Transcribing Complex Deposited 2017-06-16 | Assembly 1 Other combination Heteromer;Protein × 17 PDB declaration: eicosameric(20) Consistent with all polymers |
Chain K
1–142(142 aa)
|
Not recorded | ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 5W64 RNA Polymerase I Initial Transcribing Complex State 1 Deposited 2017-06-16 | Assembly 1 Other combination Heteromer;Protein × 17 PDB declaration: eicosameric(20) Consistent with all polymers |
Chain K
1–142(142 aa)
|
Not recorded | ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.20 Å |
| 5W65 RNA polymerase I Initial Transcribing Complex State 2 Deposited 2017-06-16 | Assembly 1 Other combination Heteromer;Protein × 17 PDB declaration: eicosameric(20) Consistent with all polymers |
Chain K
1–142(142 aa)
|
Not recorded | ZN ZINC ION × 7 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.30 Å |
| 5W66 RNA polymerase I Initial Transcribing Complex State 3 Deposited 2017-06-16 | Assembly 1 Other combination Heteromer;Protein × 17 PDB declaration: eicosameric(20) Consistent with all polymers |
Chain K
1–142(142 aa)
|
Not recorded | ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.90 Å |
| 6CNB Yeast RNA polymerase III initial transcribing complex Deposited 2018-03-08 | Assembly 1 Insufficient information Heteromer;Protein × 19 PDB declaration: 21-meric(21) Consistent with all polymers |
Chain K
1–142(142 aa)
|
Not recorded | ZN ZINC ION × 7 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.10 Å |
| 6CNC Yeast RNA polymerase III open complex Deposited 2018-03-08 | Assembly 1 Insufficient information Heteromer;Protein × 19 PDB declaration: 21-meric(21) Consistent with all polymers |
Chain K
1–142(142 aa)
|
Not recorded | ZN ZINC ION × 7 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.10 Å |
| 6CND Yeast RNA polymerase III natural open complex (nOC) Deposited 2018-03-08 | Assembly 1 Insufficient information Heteromer;Protein × 19 PDB declaration: 21-meric(21) Consistent with all polymers |
Chain K
1–142(142 aa)
|
Not recorded | ZN ZINC ION × 7 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.80 Å |
| 6CNF Yeast RNA polymerase III elongation complex Deposited 2018-03-08 | Assembly 1 Insufficient information Heteromer;Protein × 19 PDB declaration: 21-meric(21) Consistent with all polymers |
Chain K
1–142(142 aa)
|
Not recorded | ZN ZINC ION × 7 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.50 Å |
| 6EU0 RNA Polymerase III open pre-initiation complex (OC-PIC) Deposited 2017-10-27 | Assembly 1 Protein–DNA Heteromer;Protein × 20 PDB declaration: 22-meric(22) Consistent with all polymers |
Chain K
1–142(142 aa)
|
Not recorded | ZN ZINC ION × 7 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.00 Å |
| 6EU1 RNA Polymerase III - open DNA complex (OC-POL3). Deposited 2017-10-27 | Assembly 1 Protein–DNA Heteromer;Protein × 17 PDB declaration: nonadecameric(19) Consistent with all polymers |
Chain K
1–142(142 aa)
|
Not recorded | ZN ZINC ION × 6 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 6EU2 Apo RNA Polymerase III - open conformation (oPOL3) Deposited 2017-10-27 | Assembly 1 Protein heterocomplex Heteromer;Protein × 17 PDB declaration: heptadecameric(17) Consistent with protein count |
Chain K
1–142(142 aa)
|
Not recorded | ZN ZINC ION × 6 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 6EU3 Apo RNA Polymerase III - closed conformation (cPOL3) Deposited 2017-10-27 | Assembly 1 Protein heterocomplex Heteromer;Protein × 17 PDB declaration: heptadecameric(17) Consistent with protein count |
Chain K
1–142(142 aa)
|
Not recorded | ZN ZINC ION × 6 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 6F40 RNA Polymerase III open complex Deposited 2017-11-29 | Assembly 1 Protein–DNA Heteromer;Protein × 20 PDB declaration: 22-meric(22) Consistent with all polymers |
Chain K
1–142(142 aa)
|
Not recorded | ZN ZINC ION × 7 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 6F41 RNA Polymerase III initially transcribing complex Deposited 2017-11-29 | Assembly 1 Other combination Heteromer;Protein × 20 PDB declaration: 23-meric(23) Consistent with all polymers |
Chain K
1–142(142 aa)
|
Not recorded | ZN ZINC ION × 7 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.30 Å |
| 6F42 RNA Polymerase III closed complex CC1. Deposited 2017-11-29 | Assembly 1 Protein–DNA Heteromer;Protein × 20 PDB declaration: 22-meric(22) Consistent with all polymers |
Chain K
1–142(142 aa)
|
Not recorded | ZN ZINC ION × 7 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.50 Å |
| 6F44 RNA Polymerase III closed complex CC2. Deposited 2017-11-29 | Assembly 1 Protein–DNA Heteromer;Protein × 20 PDB declaration: 22-meric(22) Consistent with all polymers |
Chain K
1–142(142 aa)
|
Not recorded | ZN ZINC ION × 7 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.20 Å |
| 6H67 Yeast RNA polymerase I elongation complex stalled by cyclobutane pyrimidine dimer (CPD) Deposited 2018-07-26 | Assembly 1 Other combination Heteromer;Protein × 14 PDB declaration: heptadecameric(17) Consistent with all polymers |
Chain K
1–142(142 aa)
|
Not recorded | ZN ZINC ION × 6 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 6H68 Yeast RNA polymerase I elongation complex stalled by cyclobutane pyrimidine dimer (CPD) with fully-ordered A49 Deposited 2018-07-26 | Assembly 1 Other combination Heteromer;Protein × 14 PDB declaration: heptadecameric(17) Consistent with all polymers |
Chain K
1–142(142 aa)
|
Not recorded | ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.60 Å |
| 6HKO Yeast RNA polymerase I elongation complex bound to nucleotide analog GMPCPP Deposited 2018-09-07 | Assembly 1 Other combination Heteromer;Protein × 14 PDB declaration: heptadecameric(17) Consistent with all polymers |
Chain K
1–142(142 aa)
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 6 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE;30 seconds incubation
3 seconds blotting
blotting force 3
|
Resolution 3.42 Å |
| 6HLQ Yeast RNA polymerase I* elongation complex bound to nucleotide analog GMPCPP Deposited 2018-09-11 | Assembly 1 Other combination Heteromer;Protein × 12 PDB declaration: pentadecameric(15) Consistent with all polymers |
Chain K
1–142(142 aa)
|
Not recorded | ZN ZINC ION × 7 MG MAGNESIUM ION × 1 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE;30 seconds incubation
3 seconds blotting
blotting force 3
|
Resolution 3.18 Å |
| 6HLR Yeast RNA polymerase I elongation complex bound to nucleotide analog GMPCPP (core focused) Deposited 2018-09-11 | Assembly 1 Other combination Heteromer;Protein × 12 PDB declaration: pentadecameric(15) Consistent with all polymers |
Chain K
1–142(142 aa)
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 6 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE;30 seconds incubation
3 seconds blotting
blotting force 3
|
Resolution 3.18 Å |
| 6HLS Yeast apo RNA polymerase I* Deposited 2018-09-11 | Assembly 1 Protein heterocomplex Heteromer;Protein × 12 PDB declaration: dodecameric(12) Consistent with protein count |
Chain K
1–142(142 aa)
|
Not recorded | ZN ZINC ION × 7 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE;30 seconds incubation
3 seconds blotting
blotting force 3
|
Resolution 3.21 Å |
| 6RQH RNA Polymerase I Closed Conformation 1 (CC1) Deposited 2019-05-15 | Assembly 1 Protein–DNA Heteromer;Protein × 18 PDB declaration: eicosameric(20) Consistent with all polymers |
Chain K
1–142(142 aa)
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 6RQL RNA Polymerase I Closed Conformation 2 (CC2) Deposited 2019-05-16 | Assembly 1 Protein–DNA Heteromer;Protein × 18 PDB declaration: eicosameric(20) Consistent with all polymers |
Chain K
1–142(142 aa)
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 6RQT RNA Polymerase I-tWH-Rrn3-DNA Deposited 2019-05-16 | Assembly 1 Protein–DNA Heteromer;Protein × 15 PDB declaration: heptadecameric(17) Consistent with all polymers |
Chain K
1–142(142 aa)
|
Not recorded | ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.00 Å |
| 6RRD RNA Polymerase I Pre-initiation complex DNA opening intermediate 1 Deposited 2019-05-17 | Assembly 1 Protein–DNA Heteromer;Protein × 18 PDB declaration: eicosameric(20) Consistent with all polymers |
Chain K
1–142(142 aa)
|
Not recorded | ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 6RUI RNA Polymerase I Pre-initiation complex DNA opening intermediate 2 Deposited 2019-05-28 | Assembly 1 Protein–DNA Heteromer;Protein × 18 PDB declaration: eicosameric(20) Consistent with all polymers |
Chain K
1–142(142 aa)
|
Not recorded | ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.70 Å |
| 6RUO RNA Polymerase I Open Complex conformation 1 Deposited 2019-05-28 | Assembly 1 Protein–DNA Heteromer;Protein × 18 PDB declaration: eicosameric(20) Consistent with all polymers |
Chain K
1–142(142 aa)
|
Not recorded | ZN ZINC ION × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 6RWE RNA Polymerase I Open Complex conformation 2 Deposited 2019-06-04 | Assembly 1 Protein–DNA Heteromer;Protein × 18 PDB declaration: eicosameric(20) Consistent with all polymers |
Chain K
1–142(142 aa)
|
Not recorded | ZN ZINC ION × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 6TPS early intermediate RNA Polymerase I Pre-initiation complex - eiPIC Deposited 2019-12-14 | Assembly 1 Protein–DNA Heteromer;Protein × 18 PDB declaration: 22-meric(22) Consistent with all polymers |
Chain K
1–142(142 aa)
|
Not recorded | ZN ZINC ION × 7 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.54 Å |
| 6TUT Cryo-EM structure of the RNA Polymerase III-Maf1 complex Deposited 2020-01-08 | Assembly 1 Protein heterocomplex Heteromer;Protein × 18 PDB declaration: octadecameric(18) Consistent with protein count |
Chain K
1–142(142 aa)
|
Not recorded | ZN ZINC ION × 7 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5;15 mM HEPES, 150 mM (NH4)2SO4, 5 mM DTT
cryo-EM vitrification conditions
Cryogen ETHANE;Blot time 6, blot force 2
|
Resolution 3.25 Å |
| 7Z0H Structure of yeast RNA Polymerase III-Ty1 integrase complex at 2.6 A (focus subunit AC40). Deposited 2022-02-22 | Assembly 1 Protein heterocomplex Heteromer;Protein × 19 PDB declaration: nonadecameric(19) Consistent with protein count |
Chain K
1–142(142 aa)
|
Not recorded | ZN ZINC ION × 7 MG MAGNESIUM ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.60 Å |
| 7Z1L Structure of yeast RNA Polymerase III Pre-Termination Complex (PTC) Deposited 2022-02-24 | Assembly 1 Other combination Heteromer;Protein × 17 PDB declaration: eicosameric(20) Consistent with all polymers |
Chain K
1–142(142 aa)
|
Not recorded | ZN ZINC ION × 7 MG MAGNESIUM ION × 1 4QM (3R,5S,7R,8R,9S,10S,12S,13R,14S,17R)-10,13-dimethyl-17-[(2R)-pentan-2-yl]-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1H-cyclopenta[a]phenanthrene-3,7,12-triol × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.80 Å |
| 7Z1M Structure of yeast RNA Polymerase III Elongation Complex (EC) Deposited 2022-02-24 | Assembly 1 Other combination Heteromer;Protein × 17 PDB declaration: eicosameric(20) Consistent with all polymers |
Chain K
1–142(142 aa)
|
Not recorded | ZN ZINC ION × 7 MG MAGNESIUM ION × 1 4QM (3R,5S,7R,8R,9S,10S,12S,13R,14S,17R)-10,13-dimethyl-17-[(2R)-pentan-2-yl]-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1H-cyclopenta[a]phenanthrene-3,7,12-triol × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 7Z1N Structure of yeast RNA Polymerase III Delta C53-C37-C11 Deposited 2022-02-24 | Assembly 1 Other combination Heteromer;Protein × 14 PDB declaration: heptadecameric(17) Consistent with all polymers |
Chain K
1–142(142 aa)
|
Not recorded | ZN ZINC ION × 5 MG MAGNESIUM ION × 1 1N7 CHAPSO × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.90 Å |
| 7Z1O Structure of yeast RNA Polymerase III PTC + NTPs Deposited 2022-02-24 | Assembly 1 Other combination Heteromer;Protein × 17 PDB declaration: eicosameric(20) Consistent with all polymers |
Chain K
1–142(142 aa)
|
Not recorded | ZN ZINC ION × 7 MG MAGNESIUM ION × 1 1N7 CHAPSO × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.70 Å |
| 7Z2Z Structure of yeast RNA Polymerase III-DNA-Ty1 integrase complex (Pol III-DNA-IN1) at 3.1 A Deposited 2022-03-01 | Assembly 1 Other combination Heteromer;Protein × 19 PDB declaration: 22-meric(22) Consistent with all polymers |
Chain K
1–142(142 aa)
|
Not recorded | ZN ZINC ION × 7 MG MAGNESIUM ION × 2 4QM (3R,5S,7R,8R,9S,10S,12S,13R,14S,17R)-10,13-dimethyl-17-[(2R)-pentan-2-yl]-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1H-cyclopenta[a]phenanthrene-3,7,12-triol × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.07 Å |
| 7Z30 Structure of yeast RNA Polymerase III-Ty1 integrase complex at 2.9 A (focus subunit C11 terminal Zn-ribbon in the funnel pore). Deposited 2022-03-01 | Assembly 1 Protein heterocomplex Heteromer;Protein × 19 PDB declaration: nonadecameric(19) Consistent with protein count |
Chain K
1–142(142 aa)
|
Not recorded | ZN ZINC ION × 7 MG MAGNESIUM ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 7Z31 Structure of yeast RNA Polymerase III-Ty1 integrase complex at 2.7 A (focus subunit C11, no C11 C-terminal Zn-ribbon in the funnel pore). Deposited 2022-03-01 | Assembly 1 Protein heterocomplex Heteromer;Protein × 19 PDB declaration: nonadecameric(19) Consistent with protein count |
Chain K
1–142(142 aa)
|
Not recorded | ZN ZINC ION × 6 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.76 Å |
| 8BWS Structure of yeast RNA Polymerase III elongation complex at 3.3 A Deposited 2022-12-07 | Assembly 1 Other combination Heteromer;Protein × 17 PDB declaration: eicosameric(20) Consistent with all polymers |
Chain K
1–142(142 aa)
|
Not recorded | ZN ZINC ION × 6 MG MAGNESIUM ION × 1 4QM (3R,5S,7R,8R,9S,10S,12S,13R,14S,17R)-10,13-dimethyl-17-[(2R)-pentan-2-yl]-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1H-cyclopenta[a]phenanthrene-3,7,12-triol × 3 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 9G1V Yeast RNA polymerase I elongation complex stalled by an apurinic site Deposited 2024-07-10 | Assembly 1 Other combination Heteromer;Protein × 14 PDB declaration: 17-meric(17) Consistent with all polymers |
Chain K
1–142(142 aa)
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen METHANE
|
Resolution 2.70 Å |
| 9G1X Yeast RNA polymerase I elongation complex stalled by an apurinic site, 11-subunit Deposited 2024-07-10 | Assembly 1 Other combination Heteromer;Protein × 11 PDB declaration: 14-meric(14) Consistent with all polymers |
Chain K
1–142(142 aa)
|
Not recorded | ZN ZINC ION × 5 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen METHANE
|
Resolution 3.50 Å |
| 9G23 Yeast RNA polymerase I elongation complex stalled by an apurinic site bound to nucleotide analog AMPCPP at A-site Deposited 2024-07-10 | Assembly 1 Other combination Heteromer;Protein × 14 PDB declaration: 17-meric(17) Consistent with all polymers |
Chain K
1–142(142 aa)
|
Not recorded | ZN ZINC ION × 6 MG MAGNESIUM ION × 1 APC DIPHOSPHOMETHYLPHOSPHONIC ACID ADENOSYL ESTER × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen METHANE
|
Resolution 3.40 Å |
| 9G24 Yeast RNA polymerase I elongation complex stalled by an apurinic site bound to nucleotide analog AMPCPP at E-site Deposited 2024-07-10 | Assembly 1 Other combination Heteromer;Protein × 14 PDB declaration: heptadecameric(17) Consistent with all polymers |
Chain K
1–142(142 aa)
|
Not recorded | ZN ZINC ION × 6 APC DIPHOSPHOMETHYLPHOSPHONIC ACID ADENOSYL ESTER × 1 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen METHANE
|
Resolution 3.50 Å |
| 9G26 Yeast RNA polymerase I elongation complex stalled by an apurinic site, closed state Deposited 2024-07-10 | Assembly 1 Other combination Heteromer;Protein × 14 PDB declaration: 17-meric(17) Consistent with all polymers |
Chain K
1–142(142 aa)
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen METHANE
|
Resolution 3.40 Å |
| 9G27 Yeast RNA polymerase I elongation complex stalled by an apurinic site, pre-translocation state Deposited 2024-07-10 | Assembly 1 Other combination Heteromer;Protein × 12 PDB declaration: 15-meric(15) Consistent with all polymers |
Chain K
1–142(142 aa)
|
Not recorded | ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen METHANE
|
Resolution 2.80 Å |
| 9G29 Yeast RNA polymerase I elongation complex stalled by an apurinic site with the C-terminal of A12 in the funnel Deposited 2024-07-10 | Assembly 1 Other combination Heteromer;Protein × 14 PDB declaration: 17-meric(17) Consistent with all polymers |
Chain K
1–142(142 aa)
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 7 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen METHANE
|
Resolution 3.30 Å |
| 9G2B Yeast RNA polymerase I elongation complex stalled by an apurinic site, 12-subunit Deposited 2024-07-10 | Assembly 1 Other combination Heteromer;Protein × 12 PDB declaration: 15-meric(15) Consistent with all polymers |
Chain K
1–142(142 aa)
|
Not recorded | ZN ZINC ION × 6 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen METHANE
|
Resolution 3.20 Å |
| 9G2C Yeast RNA polymerase I elongation complex stalled by an apurinic site, open state Deposited 2024-07-10 | Assembly 1 Other combination Heteromer;Protein × 13 PDB declaration: 16-meric(16) Consistent with all polymers |
Chain K
1–142(142 aa)
|
Not recorded | ZN ZINC ION × 4 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen METHANE
|
Resolution 3.50 Å |